##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211348_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 10495020 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.10716501731297 33.0 31.0 34.0 30.0 34.0 2 32.241183532761255 34.0 31.0 34.0 30.0 34.0 3 32.30819912682396 34.0 31.0 34.0 30.0 34.0 4 35.947927874363266 37.0 35.0 37.0 35.0 37.0 5 35.852652496136265 37.0 35.0 37.0 35.0 37.0 6 35.866740701780465 37.0 35.0 37.0 35.0 37.0 7 35.777724101526246 37.0 35.0 37.0 33.0 37.0 8 35.75951651354642 37.0 35.0 37.0 33.0 37.0 9 37.37445073949359 39.0 37.0 39.0 34.0 39.0 10-11 37.32385116941178 39.0 37.0 39.0 34.0 39.0 12-13 37.255945010109556 39.0 37.0 39.0 33.5 39.0 14-15 38.495245888049766 40.0 38.0 41.0 34.0 41.0 16-17 38.47969598914533 40.0 38.0 41.0 34.0 41.0 18-19 38.48100032205751 40.0 38.0 41.0 34.0 41.0 20-21 38.446786190021555 40.0 38.0 41.0 34.0 41.0 22-23 38.33872107914039 40.0 38.0 41.0 34.0 41.0 24-25 38.250436159245055 40.0 38.0 41.0 34.0 41.0 26-27 38.155468307826 40.0 38.0 41.0 33.0 41.0 28-29 38.05335025564506 40.0 37.0 41.0 33.0 41.0 30-31 38.06621845408584 40.0 37.0 41.0 33.0 41.0 32-33 38.00853347587713 40.0 37.0 41.0 33.0 41.0 34-35 37.88416453708521 40.0 37.0 41.0 33.0 41.0 36-37 37.740609403317 40.0 37.0 41.0 33.0 41.0 38-39 37.628933675209765 40.0 37.0 41.0 33.0 41.0 40-41 37.51966323075135 40.0 36.5 41.0 32.0 41.0 42-43 37.41479473121538 40.0 36.0 41.0 32.0 41.0 44-45 37.26046258130046 40.0 36.0 41.0 31.0 41.0 46-47 37.026100903094985 39.0 36.0 41.0 31.0 41.0 48-49 35.47804892225074 39.0 35.0 41.0 24.0 41.0 50-51 36.21132222711343 39.0 35.0 40.0 29.0 41.0 52-53 36.3077414335561 39.0 35.0 40.0 29.5 41.0 54-55 36.141160760055726 38.5 35.0 40.0 29.0 41.0 56-57 35.92958574638257 38.0 35.0 40.0 28.5 41.0 58-59 35.691320978902375 38.0 34.0 40.0 28.0 41.0 60-61 35.437508837524845 37.5 34.0 40.0 28.0 41.0 62-63 35.11645246983808 37.0 34.0 40.0 27.5 41.0 64-65 34.776970696578 36.5 34.0 39.5 27.0 41.0 66-67 34.43804966546038 36.0 33.0 39.0 26.0 40.5 68-69 34.07348918820546 35.5 33.0 39.0 26.0 40.0 70-71 33.71388339421935 35.0 33.0 38.0 26.0 40.0 72-73 33.33299812673059 35.0 32.0 37.5 26.0 39.5 74-75 32.96839920266946 35.0 32.0 37.0 25.0 39.0 76-77 32.036001789420126 34.0 30.5 36.0 24.5 38.0 78-79 32.47049443450322 35.0 32.0 36.0 25.0 38.0 80-81 32.344936122084576 35.0 32.0 35.5 25.0 37.0 82-83 32.0761935184497 34.0 31.5 35.0 25.0 37.0 84-85 31.797660985877112 34.0 31.0 35.0 24.5 36.5 86-87 31.540563286206222 34.0 31.0 35.0 24.0 36.0 88-89 31.31573465319742 34.0 31.0 35.0 23.5 36.0 90-91 31.09789790776959 34.0 31.0 35.0 23.0 35.0 92-93 30.9109265156236 34.0 30.0 35.0 22.0 35.0 94-95 30.71521569277619 34.0 30.0 35.0 20.0 35.0 96-97 30.526276986608885 34.0 30.0 35.0 20.0 35.0 98-99 30.31116567667332 34.0 29.0 35.0 19.5 35.0 100 27.854763973770417 31.0 25.0 34.0 15.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.2791651646767015 1101 2 -0.2864621796100906 1101 3 -0.3123427198686528 1101 4 -0.2118211051894221 1101 5 -0.21024909159557126 1101 6 -0.23797565579418745 1101 7 -0.2501855769247996 1101 8 -0.26875632409088723 1101 9 -0.392205642974055 1101 10-11 -0.3441993500958347 1101 12-13 -0.4082179815497753 1101 14-15 -0.48663933786694 1101 16-17 -0.5260691623897671 1101 18-19 -0.4740559966269231 1101 20-21 -0.4979693029664105 1101 22-23 -0.6191112327082138 1101 24-25 -0.5457880454969342 1101 26-27 -0.6534590083964034 1101 28-29 -0.6341636955024086 1101 30-31 -0.5676387295748668 1101 32-33 -0.5754054507672564 1101 34-35 -0.6036657067252733 1101 36-37 -0.634032908228022 1101 38-39 -0.6278970134186892 1101 40-41 -0.5500505664321125 1101 42-43 -0.582657160885482 1101 44-45 -0.6382883410383471 1101 46-47 -0.5555906697013029 1101 48-49 0.6377276896244481 1101 50-51 -0.27608926832529335 1101 52-53 -0.6252637810172104 1101 54-55 -0.6518751404766334 1101 56-57 -0.6787867089179471 1101 58-59 -0.8097921732471889 1101 60-61 -0.8273227873112603 1101 62-63 -0.8755974274519929 1101 64-65 -0.8768745985293478 1101 66-67 -0.8376092628339364 1101 68-69 -0.8857040542269488 1101 70-71 -0.8327457238374265 1101 72-73 -0.8223618453167347 1101 74-75 -0.8632883262607507 1101 76-77 -0.8329613035316932 1101 78-79 -0.7847994393223416 1101 80-81 -0.6200143493196784 1101 82-83 -0.641460636579751 1101 84-85 -0.7087838434937801 1101 86-87 -0.7727465183833147 1101 88-89 -0.8082358405880825 1101 90-91 -0.858844745864257 1101 92-93 -0.8114557769353858 1101 94-95 -0.8292918621671816 1101 96-97 -1.0242734700404235 1101 98-99 -1.1930577211153341 1101 100 -1.0390554673835268 1102 1 -0.30330075017207747 1102 2 -0.2783518411642767 1102 3 -0.2992552865944802 1102 4 -0.18190391685858742 1102 5 -0.19140245568205927 1102 6 -0.26926065879814587 1102 7 -0.2516582063884414 1102 8 -0.21744362512190207 1102 9 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1.1205947206178273 2310 100 1.3117331028554524 2311 1 0.3297738163141375 2311 2 0.33009061507802073 2311 3 0.3333333788999724 2311 4 0.23419558411492147 2311 5 0.2793687602493833 2311 6 0.35137762533664585 2311 7 0.3598144213223762 2311 8 0.14946603204680997 2311 9 0.41201767265754796 2311 10-11 0.33092123080287195 2311 12-13 0.40565168328115675 2311 14-15 0.41344612965671246 2311 16-17 0.39918667489661175 2311 18-19 0.5582939431023419 2311 20-21 0.5927770672492443 2311 22-23 0.6508182390565693 2311 24-25 0.5956075343456959 2311 26-27 0.6465437610598528 2311 28-29 0.7337941863262998 2311 30-31 0.5809763056629578 2311 32-33 0.5784990268300376 2311 34-35 0.7128508045801567 2311 36-37 0.729496586744105 2311 38-39 0.7500169760338835 2311 40-41 0.7042700920814866 2311 42-43 0.7329890926098628 2311 44-45 0.7379159386129359 2311 46-47 0.8148820624601996 2311 48-49 -6.050579587156939 2311 50-51 -1.1388841913160093 2311 52-53 0.2971728900192403 2311 54-55 0.7501396915391894 2311 56-57 0.7842062915442725 2311 58-59 0.8678974551916951 2311 60-61 0.668148275288786 2311 62-63 0.6290128583436214 2311 64-65 0.7241489697187333 2311 66-67 0.8155046839900493 2311 68-69 0.8028239201734522 2311 70-71 0.8402517182717304 2311 72-73 0.3300810342096341 2311 74-75 0.6506662977759206 2311 76-77 0.7618478327292344 2311 78-79 0.6891750511809676 2311 80-81 0.7283710839713962 2311 82-83 0.6975234041656151 2311 84-85 0.7595984136866889 2311 86-87 0.7933743901175347 2311 88-89 0.8239445575861062 2311 90-91 0.8645233125944074 2311 92-93 0.8607877771113444 2311 94-95 0.8936084736907368 2311 96-97 0.9290186947997157 2311 98-99 0.9667193621949934 2311 100 1.1430519871689064 2312 1 0.058843829668759895 2312 2 0.21572479073357442 2312 3 0.17943848923258088 2312 4 -0.018031031681864818 2312 5 0.15944234059033846 2312 6 0.09240762702719962 2312 7 0.2741443893444284 2312 8 0.30576198826582157 2312 9 0.24405386615894287 2312 10-11 0.22592767154577587 2312 12-13 0.2975524721978857 2312 14-15 0.3430348309756681 2312 16-17 0.4263798095718343 2312 18-19 0.2759911413364833 2312 20-21 0.30056680212178577 2312 22-23 0.5734096926160959 2312 24-25 0.6596934731554143 2312 26-27 0.626405135150975 2312 28-29 0.7074612582768154 2312 30-31 0.7239314247348716 2312 32-33 0.7330997403628103 2312 34-35 0.3066423331015997 2312 36-37 0.6218752599969406 2312 38-39 0.7384173188250145 2312 40-41 0.5004808750316556 2312 42-43 0.5226803039405539 2312 44-45 0.6867419175326717 2312 46-47 0.752984403120216 2312 48-49 -4.641648880053975 2312 50-51 -0.7917432838169489 2312 52-53 0.5303577067842298 2312 54-55 0.7092341177661154 2312 56-57 0.847166594771636 2312 58-59 0.8918309540289897 2312 60-61 0.9058726403719533 2312 62-63 0.6800286397410744 2312 64-65 0.8574240991634738 2312 66-67 0.8640298003748939 2312 68-69 0.7547981605612222 2312 70-71 0.7448307158843406 2312 72-73 0.8112676263869574 2312 74-75 0.5862183340397138 2312 76-77 0.7835941954114141 2312 78-79 0.41244245351271047 2312 80-81 0.6450583373586127 2312 82-83 0.6345931729355883 2312 84-85 0.6935522372702252 2312 86-87 0.7376948272713548 2312 88-89 0.8127907119154294 2312 90-91 0.7025119089768381 2312 92-93 0.8726095337702056 2312 94-95 0.9202707927977265 2312 96-97 0.9670030997343098 2312 98-99 0.9994936400891277 2312 100 0.5457736560762569 2313 1 0.08399451748231712 2313 2 0.21358352893428645 2313 3 0.31981870904024845 2313 4 0.06931980570924878 2313 5 0.15543635074566708 2313 6 0.2862505989373929 2313 7 0.1452523258817422 2313 8 0.2661459514684097 2313 9 0.4475674974037247 2313 10-11 0.24856253102862524 2313 12-13 0.0421305781398118 2313 14-15 0.163882657981695 2313 16-17 0.06603940693705823 2313 18-19 0.39774999067976324 2313 20-21 0.32955678828236756 2313 22-23 0.4563821098636609 2313 24-25 0.2800306821053411 2313 26-27 0.3956623409539972 2313 28-29 0.42973590973809905 2313 30-31 0.5715347317879562 2313 32-33 0.461452723308831 2313 34-35 0.3671745701061795 2313 36-37 -0.28403252376362786 2313 38-39 0.1917951032243863 2313 40-41 0.37278734466177355 2313 42-43 0.5012980507215872 2313 44-45 0.49960120148747933 2313 46-47 0.35977244196552505 2313 48-49 -5.286981003841667 2313 50-51 -1.353766112333993 2313 52-53 0.08729899881412706 2313 54-55 0.27998696154398317 2313 56-57 0.3167662341783313 2313 58-59 0.4124014182440163 2313 60-61 0.7075341855271802 2313 62-63 0.7840306288797265 2313 64-65 0.8498595174310921 2313 66-67 0.9211981969713747 2313 68-69 0.8787786126231154 2313 70-71 0.8385035803888101 2313 72-73 0.6416932330520595 2313 74-75 0.7597399587845644 2313 76-77 0.7815198895382061 2313 78-79 0.6745140610593481 2313 80-81 -0.17169334803769942 2313 82-83 0.25061721831398387 2313 84-85 0.6388507179754583 2313 86-87 0.7465754658459574 2313 88-89 0.7194630674048916 2313 90-91 0.7648406315452121 2313 92-93 0.8149204881126586 2313 94-95 0.7409372017175606 2313 96-97 0.7831237743751238 2313 98-99 0.9716229021783391 2313 100 0.9979683254089622 2314 1 0.03844993194115176 2314 2 0.1577611431958914 2314 3 0.20188783213153272 2314 4 0.09179648660846595 2314 5 0.21137620136048696 2314 6 0.2863507923355968 2314 7 0.1076035354415481 2314 8 0.2295435275519111 2314 9 0.38300612689679525 2314 10-11 0.2758257274817453 2314 12-13 0.18364788725022407 2314 14-15 0.2262523458046033 2314 16-17 0.42104781459719476 2314 18-19 0.5194385671028883 2314 20-21 0.5752407826878638 2314 22-23 0.285499220335268 2314 24-25 0.4222494828253929 2314 26-27 0.47583929259322133 2314 28-29 0.6125766087226836 2314 30-31 0.508930655416556 2314 32-33 0.47306201396572334 2314 34-35 0.3114722678665345 2314 36-37 0.4089506407133854 2314 38-39 0.4513264800849157 2314 40-41 0.27089061835316386 2314 42-43 0.29358295736904694 2314 44-45 0.592304896101389 2314 46-47 0.6659958718989074 2314 48-49 -6.433120420034108 2314 50-51 -1.5448480090919006 2314 52-53 -0.7654093172143916 2314 54-55 -0.33222269282241257 2314 56-57 -0.06545438579512108 2314 58-59 0.4355212785043818 2314 60-61 0.6563263266482693 2314 62-63 0.5172698374749132 2314 64-65 0.7213340945916613 2314 66-67 0.7914585065194331 2314 68-69 0.7570090437912143 2314 70-71 0.5519075114216392 2314 72-73 0.668962073493482 2314 74-75 0.712870276390511 2314 76-77 0.6656982708981651 2314 78-79 0.48940287869152854 2314 80-81 0.505305049896549 2314 82-83 0.5722666868395407 2314 84-85 0.6702282844228264 2314 86-87 0.6428346411506851 2314 88-89 0.5226943297082549 2314 90-91 0.6963574589501356 2314 92-93 0.8011187685148045 2314 94-95 0.8272615491200632 2314 96-97 0.8413131997327383 2314 98-99 0.9368049601311057 2314 100 -0.5598611721580902 2315 1 0.09942889612842265 2315 2 0.20338401969488729 2315 3 0.2969818032378484 2315 4 0.12376515959272183 2315 5 0.2186449694236714 2315 6 0.279655261078922 2315 7 0.313955158312325 2315 8 0.21102292063057604 2315 9 0.4060603896189363 2315 10-11 0.20961065904698728 2315 12-13 0.4023183188829549 2315 14-15 0.23414951431266928 2315 16-17 0.37314241366588163 2315 18-19 0.4350420756720368 2315 20-21 0.3206383543083433 2315 22-23 0.4077099983397048 2315 24-25 0.4935858531354924 2315 26-27 0.5997850664334194 2315 28-29 0.6245585789407286 2315 30-31 0.5894463158073648 2315 32-33 0.6717993934300566 2315 34-35 0.6850877860685998 2315 36-37 0.6129538999846744 2315 38-39 0.6736580587068275 2315 40-41 0.7238187747065226 2315 42-43 0.7480298997719146 2315 44-45 0.7783937991958538 2315 46-47 0.6429271224033215 2315 48-49 -4.903189226310829 2315 50-51 -0.9107976079929472 2315 52-53 0.2647177192193908 2315 54-55 0.2646140583594203 2315 56-57 0.6125879048673752 2315 58-59 0.744661751850181 2315 60-61 0.5893726282161111 2315 62-63 0.2967186829727524 2315 64-65 0.5233243595742536 2315 66-67 -0.20151742349924007 2315 68-69 0.5141097919038344 2315 70-71 0.5539934787456886 2315 72-73 0.6679116257227875 2315 74-75 0.8160574373148464 2315 76-77 0.6902648496523405 2315 78-79 0.6781759392911653 2315 80-81 0.5157371072155144 2315 82-83 0.4892403631354725 2315 84-85 0.545790951006822 2315 86-87 0.5531477546123824 2315 88-89 0.5601310199977867 2315 90-91 0.6569550156087018 2315 92-93 0.7261043954721309 2315 94-95 0.37344025740467046 2315 96-97 0.4063208348587999 2315 98-99 0.3797644135089868 2315 100 0.5178768693036027 2316 1 0.24295665835023073 2316 2 0.2848843619262311 2316 3 0.2503417683924738 2316 4 0.041580354664596086 2316 5 0.19506991705190302 2316 6 0.1736868293281546 2316 7 0.2580801038664262 2316 8 0.3137173806309903 2316 9 0.4348788410739388 2316 10-11 0.4344901728710582 2316 12-13 0.3732320558418607 2316 14-15 0.44716932224829264 2316 16-17 0.43918028726844227 2316 18-19 0.1588939828340017 2316 20-21 0.48590407102375366 2316 22-23 0.6308443396999124 2316 24-25 0.625900540045663 2316 26-27 0.6238966545942972 2316 28-29 0.7473945775303221 2316 30-31 0.6548411263356471 2316 32-33 0.6119038465858395 2316 34-35 0.594040890003221 2316 36-37 0.46932060605980297 2316 38-39 0.5530588012451503 2316 40-41 0.2135218838264734 2316 42-43 0.22698628083096395 2316 44-45 0.433807318371187 2316 46-47 0.4221444289388998 2316 48-49 -5.048457183085972 2316 50-51 -1.0214134170071105 2316 52-53 0.22684809899247682 2316 54-55 0.5408841100052442 2316 56-57 0.5000473037846831 2316 58-59 0.28596148428749046 2316 60-61 0.4928268003273715 2316 62-63 0.3465731205725646 2316 64-65 0.39394340423026364 2316 66-67 0.49609558544025845 2316 68-69 0.1790164353402517 2316 70-71 -0.17043976540008288 2316 72-73 -0.07601860056472987 2316 74-75 0.1746541643386692 2316 76-77 0.15652357655173077 2316 78-79 0.26577554935484216 2316 80-81 0.19213376371894952 2316 82-83 0.02793639947422122 2316 84-85 0.2381111965837448 2316 86-87 0.31023069904645695 2316 88-89 0.2070119027472508 2316 90-91 0.23094501830507141 2316 92-93 0.2905625291705114 2316 94-95 0.5517299303144654 2316 96-97 0.5553470311129232 2316 98-99 0.5510009679652548 2316 100 -0.22940058698412003 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 3.0 10 3.0 11 19.0 12 41.0 13 226.0 14 802.0 15 2390.0 16 5674.0 17 11839.0 18 19905.0 19 30089.0 20 43192.0 21 60402.0 22 75675.0 23 81139.0 24 84849.0 25 94778.0 26 112448.0 27 138135.0 28 170558.0 29 210426.0 30 259055.0 31 322312.0 32 404781.0 33 524743.0 34 723961.0 35 1023231.0 36 1591700.0 37 2357119.0 38 1902084.0 39 243401.0 40 40.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.39355121569973 19.636567256645606 13.938886188392951 24.030995339261715 2 15.460353078779304 20.157429971438305 40.24465391276177 24.137563037020627 3 18.837451654419105 25.22223650413773 29.65659975838048 26.28371208306269 4 12.780999199544754 16.47691236039566 37.68524048776782 33.05684795229176 5 13.442048342619216 37.67897037845679 34.55467160770362 14.324309671220375 6 31.689978997653935 36.84917929630993 17.40468668086134 14.056155025174801 7 28.25326947955025 32.03230980334576 21.63302419938449 18.0813965177195 8 26.32155835587429 35.714094459445285 19.588494996330972 18.375852188349455 9 26.514307451935267 15.927229460875253 20.272543132381145 37.285919954808335 10-11 25.381237480826407 26.23350842151742 27.609752811412626 20.77550128624355 12-13 25.574527854144762 23.642075962012036 28.134711461419425 22.648684722423774 14-15 22.933783236097426 25.214091060526535 25.62247091081441 26.229654792561625 16-17 22.401507920786134 28.158597905036704 26.516470110808203 22.92342406336896 18-19 22.15318047542363 27.36824011466773 28.59956708987378 21.879012320034867 20-21 22.92271398156992 26.56019295254052 28.50018523078002 22.016907835109542 22-23 22.968557482601465 26.289784090375285 28.283320436707292 22.458337990315954 24-25 22.620510785358572 26.41544580927231 28.574204038774596 22.389839366594522 26-27 22.4878488733865 26.643302874903124 28.346621150451963 22.522227101258412 28-29 22.32601965886882 26.67723496739895 28.55187282745812 22.444872546274112 30-31 22.768854433700582 26.56382708020071 28.41675769254206 22.25056079355665 32-33 22.66119675542694 26.74428106190619 28.18012323309414 22.414398949572735 34-35 22.768840358308317 26.67922954219569 28.372325357859452 22.179604741636542 36-37 22.759706296979942 26.53136310162344 28.227296570516707 22.481634030879913 38-39 22.65269020343509 26.735249047659305 28.35417343517684 22.257887313728773 40-41 22.63603560406505 26.341608182241956 28.620880854906154 22.401475358786843 42-43 22.506002901136206 26.41575704018168 28.304049289013903 22.774190769668216 44-45 22.41794477690883 26.780583940138207 28.22241702997773 22.57905425297523 46-47 22.540475462383743 26.638122583877166 27.910280015098397 22.91112193864069 48-49 22.786736078495288 26.759185767617605 27.790010251682173 22.66406790220493 50-51 22.62574321909293 26.752647127229697 27.713929331126998 22.90768032255038 52-53 22.48736606335564 26.983548900262004 27.610672474945957 22.918412561436398 54-55 22.463442360763793 26.844970280904523 27.66703921251079 23.024548145820894 56-57 22.65492382379561 26.810720222995094 27.45439823933589 23.079957713873405 58-59 22.344029925663726 26.81617968281621 27.80079142290554 23.03899896861452 60-61 22.54215422704311 27.065860737410308 27.520047583157968 22.871937452388615 62-63 22.526571378462275 26.903289315984825 27.53959114784324 23.03054815770966 64-65 22.292813529210033 26.73563912118993 27.584602711733535 23.3869446378665 66-67 22.432994234165662 26.732610796722682 27.407168284865985 23.42722668424567 68-69 22.184207226827795 26.794313051151974 27.571243526711182 23.450236195309053 70-71 22.301301703616367 26.49733467132696 27.615270663981402 23.58609296107527 72-73 22.407834647629798 26.75846291048782 27.34448418400643 23.48921825787595 74-75 22.35125528907512 26.870902970896708 27.25112366194249 23.52671807808568 76-77 22.2343333754386 26.727649573787836 27.29956802477028 23.73844902600328 78-79 22.134180573661546 26.65947345232694 27.325211061649156 23.88113491236236 80-81 22.103103923145987 26.965266523753296 27.160017161039107 23.771612392061613 82-83 22.413343730824543 26.599493384369094 27.459926124192208 23.527236760614155 84-85 22.364975766726218 26.680999310180376 27.098140994251374 23.85588392884203 86-87 21.93931130733923 26.88981338674462 27.391973801121793 23.77890150479435 88-89 22.097032792729834 26.508424444636024 27.373427215159257 24.021115547474885 90-91 22.201053398614338 26.437105911867594 27.372686696348975 23.98915399316909 92-93 22.232077928352595 26.513390635984337 27.258113224592535 23.996418211070537 94-95 21.858589196609156 26.295482258483403 27.482433244518667 24.363495300388767 96-97 21.927022158498424 26.270838521519146 27.39709906903043 24.405040250952002 98-99 21.704088972052215 26.344416720688677 27.356171005462237 24.595323301796867 100 21.76898655632439 26.21082105129917 27.322799956841155 24.69739243553529 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1732.0 1 1261.0 2 1324.5 3 1822.5 4 1400.5 5 1175.5 6 1919.0 7 3642.0 8 5757.0 9 6892.0 10 6440.5 11 5810.0 12 6145.5 13 6933.0 14 7915.0 15 8906.0 16 9896.5 17 10886.0 18 11878.5 19 12996.0 20 14172.5 21 15685.0 22 17691.0 23 20710.5 24 24446.0 25 29194.0 26 36527.0 27 45254.0 28 54629.5 29 69019.5 30 89248.5 31 109353.0 32 135151.0 33 165855.0 34 195926.5 35 226172.0 36 261965.5 37 298532.0 38 316009.0 39 322406.0 40 321796.0 41 319016.0 42 324511.5 43 339318.5 44 359219.0 45 384801.0 46 405569.5 47 415609.5 48 427285.0 49 433012.5 50 427827.5 51 420747.5 52 411559.5 53 397083.5 54 380919.0 55 352897.5 56 318483.0 57 280294.5 58 242589.0 59 211404.0 60 176058.0 61 141302.5 62 112901.0 63 88328.0 64 64987.0 65 45912.0 66 33370.5 67 25582.0 68 19459.0 69 14007.5 70 10300.5 71 7788.0 72 5891.0 73 4369.0 74 3297.0 75 2280.5 76 1433.5 77 804.5 78 474.0 79 273.5 80 164.5 81 101.5 82 58.0 83 40.5 84 25.5 85 16.5 86 13.0 87 8.5 88 5.5 89 3.5 90 2.0 91 1.5 92 1.0 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02972838546281951 2 0.0018580240914262195 3 6.669830071786428E-5 4 0.02135298455839055 5 5.431147344168948E-4 6 1.9056657347961225E-5 7 9.528328673980613E-6 8 0.0040114263717458375 9 0.009985688450331681 10-11 0.0011243427835297122 12-13 1.0481161541378673E-4 14-15 1.0957577975077703E-4 16-17 3.7160481828524386E-4 18-19 9.528328673980613E-6 20-21 3.811331469592245E-5 22-23 2.5726487419747656E-4 24-25 4.7641643369903055E-5 26-27 5.2405807706893364E-5 28-29 0.0010767011401598091 30-31 0.0027965644658133094 32-33 0.009571206153013524 34-35 0.018427787655478502 36-37 0.021381569544412494 38-39 0.02085751146734356 40-41 0.004649824392902538 42-43 0.0048689759524040924 44-45 0.008704128243681289 46-47 0.01136729610805887 48-49 1.2004741296348174 50-51 0.01809906031622617 52-53 0.01860406173594714 54-55 0.009618847796383428 56-57 0.007603606281836529 58-59 0.00841827838346187 60-61 0.005245344935026327 62-63 0.007494030502085751 64-65 0.01896613822555841 66-67 0.02817050372462368 68-69 0.02821814536799358 70-71 0.02565978911902979 72-73 0.022844167995868515 74-75 0.009366347086522942 76-77 0.014087633944480333 78-79 0.008294410110700123 80-81 0.003025244353988844 82-83 0.013939944850033635 84-85 0.023077612048381043 86-87 0.0224582706845723 88-89 0.031629287033278636 90-91 0.02025722676088278 92-93 0.013158621898767226 94-95 0.0066174242640795345 96-97 0.004035247193430789 98-99 0.015364429986793738 100 0.033768396820587285 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1.049502E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.88549918149399 #Duplication Level Percentage of deduplicated Percentage of total 1 72.64321591728392 39.14415951851893 2 15.385704794326497 16.58132766202777 3 5.3574674072603985 8.660694167664328 4 2.3564131419894543 5.079059937357377 5 1.3254337405459298 3.57108293706561 6 0.7232168106530308 2.338253931509195 7 0.4822570448721246 1.8190663117708605 8 0.33492606847980233 1.4438126711143509 9 0.22241765384646617 1.0786577673864208 >10 1.0619260398016772 9.915895784902357 >50 0.06161782010207818 2.277062481420029 >100 0.039648118776473035 4.227644450611366 >500 0.004056292367584954 1.4508955303877693 >1k 0.0015895271343490756 1.3312164865434697 >5k 2.7405640115084293E-5 0.08662712863807542 >10k+ 8.221692034525289E-5 0.994543233082139 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 28937 0.2757212468389769 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 23473 0.22365845896434688 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14936 0.14231511707457442 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 9.528328673980611E-6 2 0.0 0.0 0.0 0.0 9.528328673980611E-6 3 0.0 0.0 0.0 0.0 9.528328673980611E-6 4 0.0 0.0 0.0 9.528328673980611E-6 9.528328673980611E-6 5 0.0 0.0 0.0 9.528328673980611E-6 9.528328673980611E-6 6 0.0 0.0 0.0 9.528328673980611E-6 9.528328673980611E-6 7 0.0 0.0 0.0 9.528328673980611E-6 9.528328673980611E-6 8 0.0 0.0 0.0 2.8584986021941836E-5 9.528328673980611E-6 9 0.0 0.0 0.0 3.8113314695922444E-5 1.9056657347961222E-5 10-11 0.0 0.0 0.0 3.8113314695922444E-5 1.9056657347961222E-5 12-13 0.0 0.0 0.0 3.8113314695922444E-5 2.8584986021941836E-5 14-15 0.0 0.0 0.0 5.2405807706893364E-5 3.334915035893214E-5 16-17 0.0 0.0 0.0 7.146246505485459E-5 6.669830071786428E-5 18-19 0.0 0.0 0.0 8.09907937288352E-5 9.051912240281581E-5 20-21 0.0 0.0 0.0 1.1433994408776733E-4 9.528328673980612E-5 22-23 0.0 0.0 0.0 3.191990105783505E-4 1.429249301097092E-4 24-25 0.0 0.0 0.0 8.718420736692259E-4 1.619815874576704E-4 26-27 0.0 0.0 0.0 0.0018484957627522387 1.71509916131651E-4 28-29 0.0 0.0 0.0 0.005959969585574872 1.71509916131651E-4 30-31 0.0 0.0 0.0 0.019418733837572488 1.7627408046864133E-4 32-33 0.0 9.528328673980611E-6 0.0 0.05148155982551725 1.8103824480563161E-4 34-35 0.0 9.528328673980611E-6 0.0 0.09921372231782312 1.8103824480563161E-4 36-37 0.0 9.528328673980611E-6 0.0 0.17469714207309753 1.8103824480563161E-4 38-39 0.0 1.9056657347961222E-5 0.0 0.28117621500483087 1.8103824480563161E-4 40-41 0.0 1.9056657347961222E-5 0.0 0.3955161590925982 1.8103824480563161E-4 42-43 0.0 1.9056657347961222E-5 0.0 0.5068022738403548 1.8580240914262193E-4 44-45 0.0 1.9056657347961222E-5 0.0 0.6437672343644891 1.9533073781660253E-4 46-47 0.0 1.9056657347961222E-5 0.0 0.8050913671436548 2.0009490215359285E-4 48-49 0.0 1.9056657347961222E-5 0.0 0.9764440658521851 2.0009490215359285E-4 50-51 0.0 1.9056657347961222E-5 0.0 1.1582826902664312 2.0962323082757345E-4 52-53 0.0 1.9056657347961222E-5 0.0 1.3398211723274467 2.286798881755347E-4 54-55 0.0 1.9056657347961222E-5 0.0 1.5396064037991353 2.477365455234959E-4 56-57 0.0 1.9056657347961222E-5 0.0 1.7718165377483797 2.477365455234959E-4 58-59 0.0 1.9056657347961222E-5 0.0 2.0289337228514097 2.525007098604862E-4 60-61 0.0 1.9056657347961222E-5 0.0 2.2933019660753384 2.572648741974765E-4 62-63 0.0 1.9056657347961222E-5 0.0 2.5574701143971144 2.6679320287145713E-4 64-65 0.0 1.9056657347961222E-5 0.0 2.830685410794834 2.763215315454377E-4 66-67 0.0 1.9056657347961222E-5 0.0 3.133995933309322 2.8584986021941834E-4 68-69 0.0 1.9056657347961222E-5 0.0 3.4669824354789225 2.8584986021941834E-4 70-71 0.0 1.9056657347961222E-5 0.0 3.819754512140044 2.9537818889339897E-4 72-73 0.0 1.9056657347961222E-5 0.0 4.167390819645889 3.287273392523311E-4 74-75 0.0 1.9056657347961222E-5 0.0 4.512902309857437 3.334915035893214E-4 76-77 0.0 1.9056657347961222E-5 0.0 4.889595255654587 3.382556679263117E-4 78-79 0.0 1.9056657347961222E-5 0.0 5.29798895095007 3.4778399660029236E-4 80-81 0.0 1.9056657347961222E-5 0.0 5.732523615962618 3.5731232527427294E-4 82-83 0.0 1.9056657347961222E-5 0.0 6.167339366671049 3.6207648961126323E-4 84-85 0.0 1.9056657347961222E-5 0.0 6.595671089716838 3.6207648961126323E-4 86-87 0.0 1.9056657347961222E-5 0.0 7.037566388630037 3.668406539482535E-4 88 0.0 1.9056657347961222E-5 0.0 7.387313220937168 3.7160481828524386E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 15910 0.0 52.001995 1 GTATCAA 28545 0.0 40.57448 1 CAACGCA 35890 0.0 31.410236 5 ATCAACG 36365 0.0 31.043982 3 TATCAAC 37610 0.0 30.375088 2 TCAACGC 37830 0.0 29.990755 4 AACGCAG 38465 0.0 29.612131 6 GTACATG 41010 0.0 27.566706 1 TACATGG 42405 0.0 26.34294 2 ACGCAGA 43860 0.0 25.723686 7 ACATGGG 42465 0.0 25.634346 3 CGCAGAG 44930 0.0 25.037992 8 GTGGTAT 7905 0.0 24.814882 1 TACCTGG 6765 0.0 24.619684 2 CATGGGG 28810 0.0 23.5338 4 TGGTATC 8345 0.0 23.10667 2 GCAGAGT 50090 0.0 22.22394 9 GTACTAG 3595 0.0 22.061022 1 ATGGGGG 19175 0.0 21.554174 5 GAGTACT 28325 0.0 20.409645 12-13 >>END_MODULE