FastQCFastQC Report
Mon 6 Jun 2016
ERR1211347_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211347_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5523115
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT201250.36437771076647874No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161610.29260661782345654No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96710.17510046414025418No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA87200.055.1196061
GTATCAA159850.041.7485541
ATCAACG197000.033.4890333
CAACGCA198150.033.295285
TATCAAC201450.032.9621472
TCAACGC202550.032.5952034
AACGCAG209600.031.6776286
TACCTGG44300.031.209852
ACGCAGA238050.027.5764187
CGCAGAG245200.026.7123818
GTACATG195150.026.6055491
GTGGTAT41550.026.3728221
GTACCTG52600.026.2865311
TACATGG197700.025.8327872
ACATGGG202150.024.290943
TGGTATC42300.024.0138322
GCAGAGT267150.023.9751959
ACCTGGG56500.023.5363853
CATGGGG142050.022.626874
ATAACGC5650.021.6235333
Produced by FastQC (version 0.11.5)