FastQCFastQC Report
Mon 6 Jun 2016
ERR1211347_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211347_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5523115
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT175810.3183167469806441No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153330.27761507772335No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99640.18040544149451893No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA88550.051.5148961
GTATCAA159600.041.2553181
ATCAACG200550.032.1929243
CAACGCA204450.031.7345895
TCAACGC207650.031.160254
TATCAAC209700.031.1414362
AACGCAG218550.030.0735826
TACCTGG46600.030.0004792
ACGCAGA251250.025.8981197
CGCAGAG257050.025.2225068
GTGGTAT43950.024.9868851
GTACCTG57650.024.9102021
GTACATG193700.024.325361
ACCTGGG58050.024.2484343
TACATGG199250.023.2154142
TGGTATC44600.022.5100632
GCAGAGT282950.022.466299
ACATGGG201150.022.3465333
ATAACGC5800.021.8424053
CATGGGG141250.020.7283524
Produced by FastQC (version 0.11.5)