##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211347_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5523115 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.7498303040947 31.0 31.0 34.0 30.0 34.0 2 31.904962688627705 31.0 31.0 34.0 30.0 34.0 3 31.959860332439213 31.0 31.0 34.0 30.0 34.0 4 35.68748034397256 37.0 35.0 37.0 33.0 37.0 5 35.56897964282837 37.0 35.0 37.0 33.0 37.0 6 35.591317037577525 37.0 35.0 37.0 33.0 37.0 7 35.496390714298 37.0 35.0 37.0 33.0 37.0 8 35.470350155663965 37.0 35.0 37.0 33.0 37.0 9 37.02099539843005 39.0 37.0 39.0 33.0 39.0 10-11 36.94501662558176 39.0 37.0 39.0 33.0 39.0 12-13 36.866486575057735 39.0 37.0 39.0 33.0 39.0 14-15 37.99142965518553 40.0 37.0 41.0 33.0 41.0 16-17 37.96380050026117 40.0 37.0 41.0 33.0 41.0 18-19 37.96183358485203 40.0 37.0 41.0 33.0 41.0 20-21 37.9205040090601 40.0 37.0 41.0 33.0 41.0 22-23 37.799387845445914 40.0 37.0 41.0 32.5 41.0 24-25 37.695796484411424 39.0 37.0 41.0 32.0 41.0 26-27 37.579393965180884 39.0 37.0 41.0 32.0 41.0 28-29 37.45192558909238 39.0 36.0 41.0 32.0 41.0 30-31 37.45061717889271 39.0 36.0 41.0 32.0 41.0 32-33 37.369192928266024 39.0 36.0 41.0 31.5 41.0 34-35 37.214471905799535 39.0 36.0 41.0 31.0 41.0 36-37 37.045664357884995 39.0 36.0 41.0 31.0 41.0 38-39 36.91259434214207 39.0 35.0 40.0 31.0 41.0 40-41 36.79262064976014 39.0 35.0 40.0 30.0 41.0 42-43 36.66770065443142 39.0 35.0 40.0 30.0 41.0 44-45 36.49430058943187 39.0 35.0 40.0 30.0 41.0 46-47 36.228895650371214 38.0 35.0 40.0 29.5 41.0 48-49 34.71737162814824 38.0 34.0 40.0 23.0 41.0 50-51 35.391739534664765 38.0 33.5 40.0 26.5 41.0 52-53 35.45733829550896 38.0 34.0 40.0 27.0 41.0 54-55 35.270289863600524 38.0 34.0 40.0 27.0 41.0 56-57 35.04105110974514 38.0 34.0 40.0 26.0 41.0 58-59 34.793164998375005 37.0 33.0 40.0 26.0 41.0 60-61 34.52947304193376 37.0 33.0 40.0 26.0 41.0 62-63 34.194643602387416 36.0 33.0 39.0 26.0 40.5 64-65 33.84455674017289 36.0 32.0 39.0 25.0 40.0 66-67 33.49492378847806 35.0 32.0 39.0 24.5 40.0 68-69 33.11909411627315 35.0 31.0 38.0 23.5 40.0 70-71 32.73988084622536 35.0 31.0 37.5 23.0 40.0 72-73 32.34310275994615 35.0 31.0 37.0 22.5 39.0 74-75 31.962239243615244 34.0 30.5 36.0 22.0 39.0 76-77 31.142701265499632 33.5 29.5 35.0 21.5 38.0 78-79 31.619349044877755 34.0 30.0 35.5 22.5 37.5 80-81 31.499243814405457 34.0 30.0 35.0 22.5 37.0 82-83 31.218732997592845 34.0 30.0 35.0 21.5 37.0 84-85 30.919135397325604 34.0 30.0 35.0 20.5 36.0 86-87 30.630933268635545 34.0 29.0 35.0 20.0 36.0 88-89 30.376477404508144 34.0 29.0 35.0 20.0 35.0 90-91 30.130908192206753 33.5 29.0 35.0 19.0 35.0 92-93 29.904944401845697 33.0 29.0 35.0 18.5 35.0 94-95 29.664248526420327 33.0 29.0 35.0 18.0 35.0 96-97 29.423984744116318 33.0 28.0 34.5 18.0 35.0 98-99 29.158010923183745 33.0 27.0 34.0 17.5 35.0 100 26.6167432689705 30.0 23.0 34.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.45375708383925684 1101 2 -0.40678193833868903 1101 3 -0.43599711623468806 1101 4 -0.28045707634939987 1101 5 -0.31097408116529124 1101 6 -0.3841461771719068 1101 7 -0.39239510885807505 1101 8 -0.40796654446585023 1101 9 -0.5872771494858142 1101 10-11 -0.5635561580602655 1101 12-13 -0.6561616048464032 1101 14-15 -0.7147941226168157 1101 16-17 -0.7368030656790978 1101 18-19 -0.7311246162499714 1101 20-21 -0.7732894052352037 1101 22-23 -0.9221883128044439 1101 24-25 -0.7999996356936663 1101 26-27 -0.9262244477370629 1101 28-29 -0.9616572028695032 1101 30-31 -0.9028820341961321 1101 32-33 -0.8950877706987441 1101 34-35 -0.9106473276129776 1101 36-37 -0.9802479758570612 1101 38-39 -0.879837695567609 1101 40-41 -0.9617463245730207 1101 42-43 -1.0476907361059702 1101 44-45 -1.0068034947947808 1101 46-47 -1.0059342933599922 1101 48-49 0.01702383163914334 1101 50-51 -0.8107047179332838 1101 52-53 -1.0945544284094666 1101 54-55 -1.0979618267536466 1101 56-57 -1.0175467255494723 1101 58-59 -1.2334356615823339 1101 60-61 -1.330524663328454 1101 62-63 -1.2301368364415808 1101 64-65 -1.4550424520305398 1101 66-67 -1.3747761283699553 1101 68-69 -1.4042699286110256 1101 70-71 -1.2228563006064128 1101 72-73 -1.3044502684173835 1101 74-75 -1.5011885878394047 1101 76-77 -1.2738860553000926 1101 78-79 -1.1611835386035168 1101 80-81 -1.1117827523198933 1101 82-83 -1.106456135195419 1101 84-85 -1.0813463772551195 1101 86-87 -1.0097464203600737 1101 88-89 -1.1322874585141918 1101 90-91 -1.2142665256822092 1101 92-93 -1.1800033056735302 1101 94-95 -1.302105382161571 1101 96-97 -1.4796022652436918 1101 98-99 -1.6414439722030423 1101 100 -1.6111469432721641 1102 1 -0.362667530359019 1102 2 -0.3829592472307439 1102 3 -0.3868450316786145 1102 4 -0.3708499703642758 1102 5 -0.21135207049168514 1102 6 -0.38128812031418136 1102 7 -0.367187759470994 1102 8 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-1.0934724628717092 1102 72-73 -1.2477517230219277 1102 74-75 -1.4141842888091105 1102 76-77 -1.2202601584624304 1102 78-79 -1.096593654344968 1102 80-81 -1.0209353623725086 1102 82-83 -1.0636870345258451 1102 84-85 -0.8944837196249864 1102 86-87 -1.0106948453850215 1102 88-89 -0.9573372133298932 1102 90-91 -0.978244440769668 1102 92-93 -1.2441125446892727 1102 94-95 -1.3659557011664205 1102 96-97 -1.4071363760776094 1102 98-99 -1.5256504121798748 1102 100 -1.3019197595704561 1103 1 -0.4850992823808653 1103 2 -0.41123170381782614 1103 3 -0.37192610024660766 1103 4 -0.2988466543410979 1103 5 -0.3665856476123466 1103 6 -0.3885295679979137 1103 7 -0.40171105342965063 1103 8 -0.41946342040306916 1103 9 -0.5889282189823462 1103 10-11 -0.4911489004810221 1103 12-13 -0.524877650062912 1103 14-15 -0.656271176113215 1103 16-17 -0.6914282174456119 1103 18-19 -0.7599412155725602 1103 20-21 -0.8182996077180746 1103 22-23 -0.8052600601364048 1103 24-25 -0.9191406533603299 1103 26-27 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1104 48-49 0.3005888448212417 1104 50-51 -0.5520490915120106 1104 52-53 -0.9725782501753741 1104 54-55 -1.0898347775608528 1104 56-57 -1.0710016067144679 1104 58-59 -1.0966159242914273 1104 60-61 -1.1418306213559788 1104 62-63 -1.1669298765470302 1104 64-65 -1.1732640121332665 1104 66-67 -1.13836143783589 1104 68-69 -1.1527159309969548 1104 70-71 -1.2064683782474717 1104 72-73 -1.2112162006114353 1104 74-75 -1.2120920006507383 1104 76-77 -1.058972909467375 1104 78-79 -0.863844332734498 1104 80-81 -0.8688793526923497 1104 82-83 -0.9516902101003346 1104 84-85 -1.04850643055822 1104 86-87 -0.9444679143747976 1104 88-89 -1.0705397588329362 1104 90-91 -1.235996969803704 1104 92-93 -1.163404822891735 1104 94-95 -1.240395036566973 1104 96-97 -1.3218146311704118 1104 98-99 -1.2302073812215006 1104 100 -1.068436517222608 1105 1 -0.37514422253574864 1105 2 -0.33169628496267123 1105 3 -0.4143339240260815 1105 4 -0.27419953518671036 1105 5 -0.34286023941754706 1105 6 -0.35436401468707857 1105 7 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1105 70-71 -1.0367985544349771 1105 72-73 -0.9386437225019932 1105 74-75 -1.0898083889570849 1105 76-77 -0.9605031983208363 1105 78-79 -0.8516145385474196 1105 80-81 -0.8556667876691719 1105 82-83 -0.8323626427337274 1105 84-85 -0.8137303411955585 1105 86-87 -0.8339402699652751 1105 88-89 -0.910514285931022 1105 90-91 -0.8907464884271477 1105 92-93 -0.9122637521749013 1105 94-95 -0.9282849519841108 1105 96-97 -0.9410204729926193 1105 98-99 -1.0539889295720464 1105 100 -1.0995126803330564 1106 1 -0.4646463254632529 1106 2 -0.4443284194702706 1106 3 -0.44484414244944404 1106 4 -0.32841086581219514 1106 5 -0.35801906294884844 1106 6 -0.44987727887376394 1106 7 -0.3994711854947042 1106 8 -0.4341693801338167 1106 9 -0.6152588242703132 1106 10-11 -0.4690472096450904 1106 12-13 -0.5541283029369382 1106 14-15 -0.6695846335500377 1106 16-17 -0.6964818171042495 1106 18-19 -0.7583107066244565 1106 20-21 -0.7674465305962741 1106 22-23 -0.8251972652483204 1106 24-25 -0.7661677003194001 1106 26-27 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2208 80-81 1.1623912977717517 2208 82-83 1.2213474423083674 2208 84-85 1.2795656062560141 2208 86-87 1.3430862754031452 2208 88-89 1.3332357082799113 2208 90-91 1.2923966153545905 2208 92-93 1.3540160284016096 2208 94-95 1.369997145176093 2208 96-97 1.4331894834350365 2208 98-99 1.5202746956328461 2208 100 1.7809781394884396 2209 1 0.36345402397754256 2209 2 0.4194056563069104 2209 3 0.4451230776480912 2209 4 0.3275634859860759 2209 5 0.43204879752371284 2209 6 0.4210250488706677 2209 7 0.4997709467999556 2209 8 0.5350242684495896 2209 9 0.7138959465962245 2209 10-11 0.6674880377879617 2209 12-13 0.6994708862099159 2209 14-15 0.8605919700497182 2209 16-17 0.807491169049193 2209 18-19 0.7968895000298346 2209 20-21 0.7781446997899479 2209 22-23 0.8343325925234524 2209 24-25 0.8601310671326559 2209 26-27 0.9216098376359199 2209 28-29 1.0192239118198003 2209 30-31 0.9784907219431531 2209 32-33 0.9714621735151567 2209 34-35 0.9995555200403032 2209 36-37 1.0949809198591893 2209 38-39 1.0695438582861172 2209 40-41 1.0234557132106872 2209 42-43 1.0747581430914792 2209 44-45 1.0684031927791793 2209 46-47 1.1229021948582414 2209 48-49 2.2764610899674196 2209 50-51 1.5206560293586833 2209 52-53 1.1979165399806888 2209 54-55 1.2525757621406086 2209 56-57 1.1967816198730574 2209 58-59 1.2438543616976148 2209 60-61 1.1858404439723031 2209 62-63 1.221342184709549 2209 64-65 1.2836539515284855 2209 66-67 1.2760909319186382 2209 68-69 1.3502660686364294 2209 70-71 1.268096522972236 2209 72-73 1.3136418288120169 2209 74-75 1.2861612525340398 2209 76-77 1.1743809471840514 2209 78-79 1.1335327457351063 2209 80-81 1.090447565223645 2209 82-83 1.009556764255347 2209 84-85 1.0715064294554608 2209 86-87 1.0413946447474913 2209 88-89 1.1025596128012758 2209 90-91 1.2244433672669537 2209 92-93 1.1449201648150122 2209 94-95 1.1883989445033691 2209 96-97 1.2508537786421243 2209 98-99 1.3399393565304827 2209 100 1.5522888499514274 2210 1 0.6281294828049049 2210 2 0.5810275355538046 2210 3 0.6002878527480604 2210 4 0.43187185802776185 2210 5 0.5138935146342263 2210 6 0.525422974852134 2210 7 0.5869929342584399 2210 8 0.5907187280181887 2210 9 0.740973116228858 2210 10-11 0.7457422066679271 2210 12-13 0.7627272597073258 2210 14-15 0.9168579958252181 2210 16-17 0.938603738328446 2210 18-19 0.9093952732046731 2210 20-21 0.9461009816081045 2210 22-23 0.9421417513950416 2210 24-25 0.9512356039882661 2210 26-27 0.9345416916288372 2210 28-29 0.9830566484803214 2210 30-31 1.0662280998167049 2210 32-33 1.0657320906110286 2210 34-35 1.1291968460231914 2210 36-37 1.12634426552615 2210 38-39 1.2096241308878533 2210 40-41 1.2316329745041372 2210 42-43 1.17120736741375 2210 44-45 1.1713982222934831 2210 46-47 1.2523253296398522 2210 48-49 2.423623235109062 2210 50-51 1.6877146510679992 2210 52-53 1.4109652244068087 2210 54-55 1.4722759645947576 2210 56-57 1.5160323214826477 2210 58-59 1.4324872305614207 2210 60-61 1.3914650952651684 2210 62-63 1.4169945149699146 2210 64-65 1.400172755458506 2210 66-67 1.4329060466689256 2210 68-69 1.5228375248429629 2210 70-71 1.485713230542494 2210 72-73 1.4661262556469765 2210 74-75 1.4925722150796084 2210 76-77 1.410834167663591 2210 78-79 1.2948595003768624 2210 80-81 1.3513317459085918 2210 82-83 1.310003535836973 2210 84-85 1.3361025806046172 2210 86-87 1.364590929280162 2210 88-89 1.4554837252767854 2210 90-91 1.51044916842595 2210 92-93 1.4435072572085836 2210 94-95 1.5064236561350768 2210 96-97 1.659096201087955 2210 98-99 1.6866877855215634 2210 100 1.8245343713093405 2211 1 0.5943030647227907 2211 2 0.5742410749557756 2211 3 0.5478863102449267 2211 4 0.41936811281829023 2211 5 0.45469830089592733 2211 6 0.4983317012880235 2211 7 0.5657261495101977 2211 8 0.5986477220310391 2211 9 0.6708905228160802 2211 10-11 0.7039097067204665 2211 12-13 0.7195424241003039 2211 14-15 0.8823096610365653 2211 16-17 0.8381477576531395 2211 18-19 0.8857646050411034 2211 20-21 0.9013474684810632 2211 22-23 0.8594812364709341 2211 24-25 0.9092011493348906 2211 26-27 1.0100210602445259 2211 28-29 1.0126436099924447 2211 30-31 0.9850455949059622 2211 32-33 0.9919718447960904 2211 34-35 1.0596547319444483 2211 36-37 1.0459042060286166 2211 38-39 1.0558050681105442 2211 40-41 1.0813137434482982 2211 42-43 0.9595889747116217 2211 44-45 0.9522065679258418 2211 46-47 1.0984038735406187 2211 48-49 2.2646907728934806 2211 50-51 1.5653799595872755 2211 52-53 1.2421049090512923 2211 54-55 1.292279240999079 2211 56-57 1.2252143490810568 2211 58-59 1.225696757482666 2211 60-61 1.196517399907627 2211 62-63 1.2012633977633485 2211 64-65 1.24222875331197 2211 66-67 1.2976403967885304 2211 68-69 1.3377217947993927 2211 70-71 1.3525441928272741 2211 72-73 1.3492826349408702 2211 74-75 1.4174586782525473 2211 76-77 1.0836118834192767 2211 78-79 0.9672324651006043 2211 80-81 1.007859369654728 2211 82-83 1.1075470309915652 2211 84-85 1.1838010384927244 2211 86-87 1.2367450477549795 2211 88-89 1.3264745200454904 2211 90-91 1.2756068830297522 2211 92-93 1.269407754328558 2211 94-95 1.2781141624873982 2211 96-97 1.4091185495137637 2211 98-99 1.4683221244226026 2211 100 1.6912061195417323 2212 1 0.6240135761570365 2212 2 0.6018499151134513 2212 3 0.6031751947237538 2212 4 0.4521096247077736 2212 5 0.47133710606250645 2212 6 0.37141005021506857 2212 7 0.34528540951978215 2212 8 0.46139365776630115 2212 9 0.6168548316142974 2212 10-11 0.670065908442325 2212 12-13 0.6140750468620197 2212 14-15 0.8357717682479873 2212 16-17 0.8574817552524081 2212 18-19 0.8442275414364957 2212 20-21 0.8926431040333398 2212 22-23 0.91344204845565 2212 24-25 0.9676140590631803 2212 26-27 1.000460178617537 2212 28-29 0.9320829897536456 2212 30-31 1.0151357853150884 2212 32-33 1.0259086678684426 2212 34-35 0.927397132737525 2212 36-37 0.9863508812305923 2212 38-39 1.1101120242060531 2212 40-41 1.205919191990894 2212 42-43 1.1575487227683183 2212 44-45 1.1951039526198883 2212 46-47 1.2054239609242074 2212 48-49 2.3930036614442685 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0.7735730790954207 2311 34-35 0.9683454724283536 2311 36-37 0.9977210980047104 2311 38-39 1.0903294454184973 2311 40-41 0.9666380738578582 2311 42-43 1.0082573796624246 2311 44-45 1.0889922709711612 2311 46-47 1.024132934324193 2311 48-49 -5.525270630466025 2311 50-51 -0.8380777824579582 2311 52-53 0.5184622273162773 2311 54-55 1.06193610684673 2311 56-57 1.1968061425999252 2311 58-59 1.2079394437599547 2311 60-61 1.0399374041939708 2311 62-63 0.9168174553679052 2311 64-65 1.072179465036605 2311 66-67 1.181736019247701 2311 68-69 1.2445090576659297 2311 70-71 1.2146567694939776 2311 72-73 0.8721622263115094 2311 74-75 1.0373795320246622 2311 76-77 1.0900327324070211 2311 78-79 1.0073000873278168 2311 80-81 0.9903928651725771 2311 82-83 1.0070148241419723 2311 84-85 1.0676264994556348 2311 86-87 1.0893588319861678 2311 88-89 1.2032442513515882 2311 90-91 1.2073147858126525 2311 92-93 1.1295054754562734 2311 94-95 1.2197433121680916 2311 96-97 1.2798625288978371 2311 98-99 1.3414170990890533 2311 100 1.5234538150009946 2312 1 0.20063261259495846 2312 2 0.34615283081305037 2312 3 0.34200084292230315 2312 4 0.11386912633691537 2312 5 0.31833262431891995 2312 6 0.2391835266290343 2312 7 0.3678289071118641 2312 8 0.42258466000831163 2312 9 0.383796553859888 2312 10-11 0.31247233761389737 2312 12-13 0.4271796104163954 2312 14-15 0.5465542217550592 2312 16-17 0.6471524402523414 2312 18-19 0.49632998960358066 2312 20-21 0.5159438598905979 2312 22-23 0.7732685430516 2312 24-25 0.8988416437780131 2312 26-27 0.8085867858195002 2312 28-29 0.8974184159773131 2312 30-31 1.015536823839163 2312 32-33 1.0475233241358595 2312 34-35 0.6188803987079723 2312 36-37 0.94474633377434 2312 38-39 1.0118989088048949 2312 40-41 0.843819932464001 2312 42-43 0.8518322533676113 2312 44-45 1.0496446732525726 2312 46-47 1.125750255674454 2312 48-49 -4.0198178446204125 2312 50-51 -0.3643784468087503 2312 52-53 0.8487213437697108 2312 54-55 1.139370566899899 2312 56-57 1.252856129477614 2312 58-59 1.3216121182895932 2312 60-61 1.3098858544720215 2312 62-63 1.0842499725150603 2312 64-65 1.3234839808850083 2312 66-67 1.2524889651404862 2312 68-69 1.2528851716125189 2312 70-71 1.353972999101451 2312 72-73 1.4726256011132293 2312 74-75 1.1521761694010202 2312 76-77 1.1554673458101412 2312 78-79 0.87511524573382 2312 80-81 1.0992997403383775 2312 82-83 1.0701686765033678 2312 84-85 1.11129711038717 2312 86-87 1.2716324059321984 2312 88-89 1.2497447605065553 2312 90-91 1.0481100260005647 2312 92-93 1.2511791836781754 2312 94-95 1.4133801293141346 2312 96-97 1.5449939685012701 2312 98-99 1.695880020467147 2312 100 1.3025532342827155 2313 1 0.11940731763501944 2313 2 0.29000667423144577 2313 3 0.39466858764826895 2313 4 0.18114806022229857 2313 5 0.2623435818078974 2313 6 0.39412165006118016 2313 7 0.28664748665806883 2313 8 0.41162651861721145 2313 9 0.5868303629531795 2313 10-11 0.3858409620807919 2313 12-13 0.26329680795992516 2313 14-15 0.26806786565465757 2313 16-17 0.3227684227073908 2313 18-19 0.6336646033362001 2313 20-21 0.5460835485824589 2313 22-23 0.7171480284742415 2313 24-25 0.4531896795678705 2313 26-27 0.5875710814491342 2313 28-29 0.5974132805863661 2313 30-31 0.7536609410190991 2313 32-33 0.723027663098506 2313 34-35 0.572610910726489 2313 36-37 -0.20745251061357095 2313 38-39 0.35415849060792226 2313 40-41 0.6122142201384477 2313 42-43 0.7080431126131188 2313 44-45 0.7697011499708211 2313 46-47 0.6357958166578968 2313 48-49 -4.797544295672424 2313 50-51 -1.0400847180111725 2313 52-53 0.2820795338864457 2313 54-55 0.46820954774194945 2313 56-57 0.5319741689549815 2313 58-59 0.641985195121805 2313 60-61 0.9263567308637661 2313 62-63 1.0760485621092855 2313 64-65 1.2087215423460194 2313 66-67 1.2785052191654458 2313 68-69 1.2180990142778398 2313 70-71 1.102593188988827 2313 72-73 0.8312969743517229 2313 74-75 1.054317403743024 2313 76-77 1.0474558376879521 2313 78-79 0.9148829070399636 2313 80-81 -0.053090947249923914 2313 82-83 0.4007039091298168 2313 84-85 0.9014179696228872 2313 86-87 1.073401538793476 2313 88-89 1.0215782455948883 2313 90-91 1.0730780567938965 2313 92-93 1.1089423569695178 2313 94-95 1.0402255788847583 2313 96-97 0.9635141641251792 2313 98-99 1.2685992067117944 2313 100 1.4040155811256199 2314 1 0.15243073118346828 2314 2 0.2979615945376999 2314 3 0.28597196654533263 2314 4 0.17527041257955034 2314 5 0.3126548893895773 2314 6 0.3667147249851439 2314 7 0.17871312877828416 2314 8 0.3691329640407375 2314 9 0.5886557130689667 2314 10-11 0.5190771934033194 2314 12-13 0.493929654583134 2314 14-15 0.4501421402164212 2314 16-17 0.6023245382648597 2314 18-19 0.6885614794390236 2314 20-21 0.7845855024506108 2314 22-23 0.5885979550269695 2314 24-25 0.7461426294330238 2314 26-27 0.7102430350572462 2314 28-29 0.8594825346636696 2314 30-31 0.7560449225318493 2314 32-33 0.746544507392791 2314 34-35 0.5847963999831336 2314 36-37 0.7083325941323793 2314 38-39 0.7464148885641961 2314 40-41 0.6259600306333084 2314 42-43 0.613917655276488 2314 44-45 0.8173746762233378 2314 46-47 1.0008598970518037 2314 48-49 -5.721019130609257 2314 50-51 -1.1190985424711641 2314 52-53 -0.5769121581515648 2314 54-55 -0.17843954953251284 2314 56-57 0.1860116890374357 2314 58-59 0.6871193181380022 2314 60-61 0.934367186075626 2314 62-63 0.8092329508718734 2314 64-65 0.9634875071744844 2314 66-67 1.061413917776683 2314 68-69 1.0455246521524302 2314 70-71 0.8138120023444841 2314 72-73 1.0362622601985905 2314 74-75 1.0699241633568342 2314 76-77 0.9063543112427759 2314 78-79 0.7056331873000623 2314 80-81 0.8140109025461406 2314 82-83 0.8392900199167244 2314 84-85 0.8836533414788335 2314 86-87 0.9925253255913091 2314 88-89 0.9646704116328735 2314 90-91 1.0629990818277477 2314 92-93 1.1185284855616864 2314 94-95 1.2239362904439908 2314 96-97 1.297221868599415 2314 98-99 1.3466090148993892 2314 100 -0.13632253500461644 2315 1 0.19224292023053735 2315 2 0.3078845014370266 2315 3 0.4343503474195174 2315 4 0.2437501788703642 2315 5 0.3593106313699792 2315 6 0.4295376874220196 2315 7 0.49060648548328345 2315 8 0.38252992956734033 2315 9 0.6121299834366738 2315 10-11 0.3679379086904362 2315 12-13 0.5566111730212455 2315 14-15 0.4178761651480656 2315 16-17 0.5598293206134315 2315 18-19 0.714453286707851 2315 20-21 0.5765882720526037 2315 22-23 0.634970529176691 2315 24-25 0.7026096738557897 2315 26-27 0.7537384230337878 2315 28-29 0.7818638140496503 2315 30-31 0.8346640816444122 2315 32-33 0.8589296871865244 2315 34-35 0.8891144750407278 2315 36-37 0.8389109047870988 2315 38-39 0.9192489030114146 2315 40-41 0.9788282131748147 2315 42-43 1.010623799871503 2315 44-45 0.9694046232210738 2315 46-47 0.8910087580282706 2315 48-49 -4.304913890864071 2315 50-51 -0.6255951489345222 2315 52-53 0.45089072299191457 2315 54-55 0.4802873178267646 2315 56-57 0.8243762871960953 2315 58-59 0.9673784114562025 2315 60-61 0.8902328573944303 2315 62-63 0.6827672431187963 2315 64-65 0.91080172221357 2315 66-67 0.194105306862852 2315 68-69 0.8368312784895409 2315 70-71 0.9061969587380858 2315 72-73 1.067231896529286 2315 74-75 1.0934087875511729 2315 76-77 1.008009102256846 2315 78-79 0.8949059652989 2315 80-81 0.8804064248799115 2315 82-83 0.8003507587284808 2315 84-85 0.9087705122835779 2315 86-87 0.9069882245657581 2315 88-89 0.8069792767284838 2315 90-91 0.977163177294134 2315 92-93 1.0987596375396294 2315 94-95 0.7182872053211682 2315 96-97 0.8928996450153406 2315 98-99 0.8488719556435989 2315 100 0.9236299455950174 2316 1 0.2870763724118319 2316 2 0.33801653895872263 2316 3 0.30830134692399724 2316 4 0.10092429757303734 2316 5 0.27071782249062437 2316 6 0.22813906385771077 2316 7 0.3728211537849404 2316 8 0.4085732940062101 2316 9 0.5503166023690937 2316 10-11 0.5278904157697397 2316 12-13 0.37104595343635793 2316 14-15 0.584306011153366 2316 16-17 0.6050753443332653 2316 18-19 0.24421891021326303 2316 20-21 0.6223179791539621 2316 22-23 0.6979953810918857 2316 24-25 0.7724000678771077 2316 26-27 0.7937858174876098 2316 28-29 0.8831965492044986 2316 30-31 0.7180885562345196 2316 32-33 0.6714708557201945 2316 34-35 0.696630925574155 2316 36-37 0.5410545944104896 2316 38-39 0.6769390022322739 2316 40-41 0.3270456906896655 2316 42-43 0.28253854180763227 2316 44-45 0.4528381802086443 2316 46-47 0.5174884807905471 2316 48-49 -4.374753518325413 2316 50-51 -0.7261544033020471 2316 52-53 0.44130841855933056 2316 54-55 0.7012745673973484 2316 56-57 0.5771841406143636 2316 58-59 0.5202916760140113 2316 60-61 0.7160486508067834 2316 62-63 0.5312660780211615 2316 64-65 0.599375339429109 2316 66-67 0.6606905476715781 2316 68-69 0.33769016757307213 2316 70-71 0.012549254104214924 2316 72-73 0.15958199055108935 2316 74-75 0.4253936247769765 2316 76-77 0.4385458527222674 2316 78-79 0.4882905007846787 2316 80-81 0.42570958283466354 2316 82-83 0.3639437410970956 2316 84-85 0.4989662681922482 2316 86-87 0.5013211133289417 2316 88-89 0.34092892207891623 2316 90-91 0.3934568107820233 2316 92-93 0.5345118035296608 2316 94-95 0.703787441454125 2316 96-97 0.8403707998461627 2316 98-99 0.8699474784277079 2316 100 0.17198104735500408 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 4.0 10 8.0 11 13.0 12 35.0 13 161.0 14 651.0 15 1868.0 16 4238.0 17 8522.0 18 14263.0 19 20976.0 20 29181.0 21 40044.0 22 48735.0 23 53314.0 24 57628.0 25 65465.0 26 78774.0 27 97070.0 28 118734.0 29 145158.0 30 177395.0 31 217130.0 32 270593.0 33 344018.0 34 455438.0 35 614860.0 36 865764.0 37 1078515.0 38 662456.0 39 52102.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.40375736587039 20.072584283378255 14.624688110162651 23.898970240588703 2 15.779741266148234 20.798689130100218 39.41651804709355 24.005051556658007 3 18.86061698549658 25.297060787469093 30.482633703607675 25.359688523426648 4 13.190178605382247 17.017219492849808 37.15363177556893 32.63897012619902 5 13.412175729293368 37.28495265147057 34.51700733973724 14.785864279498817 6 31.51416502658828 36.70279969075394 17.505065081086563 14.277970201571216 7 27.691642794264247 32.30525388395025 21.714996286515177 18.288107035270322 8 26.192108780078954 35.75476246907661 19.630927318740458 18.42220143210398 9 26.376243138339905 16.023022005839156 20.23683636819275 37.36389848762819 10-11 25.29889675308674 26.24217802410656 27.738721335867915 20.720203886938783 12-13 25.49335197983093 23.778521179324585 28.135712656844365 22.59241418400012 14-15 22.870596206084727 25.33334045492872 25.889005952627752 25.9070573863588 16-17 22.337086455490816 28.095791658673708 26.75456793619442 22.812553949641057 18-19 22.08983717429722 27.19551622549198 28.893036709631858 21.821609890578948 20-21 22.915405300490015 26.442538192236555 28.81228915872066 21.82976734855277 22-23 22.78289778980967 26.238050413363943 28.655950219251906 22.323101577574477 24-25 22.435666703843776 26.277259994003384 28.90824049919683 22.378832802956016 26-27 22.38393862011127 26.463269258345655 28.681556702091136 22.471235419451933 28-29 22.22848184048831 26.746080430833945 28.790075639283756 22.235362089393988 30-31 22.71037310439916 26.501257924835937 28.79819517887519 21.99017379188971 32-33 22.661574176053254 26.586534722559847 28.50653079690217 22.24536030448473 34-35 22.69631642237058 26.671126220556147 28.633957698040817 21.998599659032458 36-37 22.684703283171256 26.58230564459173 28.522744970210606 22.210246102026403 38-39 22.61814129980873 26.69342171075578 28.545651507024356 22.14278548241114 40-41 22.67689083333145 26.392762810951748 28.72600938372085 22.204336971995954 42-43 22.46427215045192 26.548249062640465 28.463445581090486 22.52403320581713 44-45 22.36942623721115 26.800406613959193 28.473859535301955 22.3563076135277 46-47 22.421931678125894 26.59595417008862 28.147999733094338 22.834114418691147 48-49 22.69690405267926 26.752551798242656 28.020709035630624 22.52983511344746 50-51 22.569610504236454 26.873988965611655 27.917641747632416 22.638758782519474 52-53 22.453981730773148 27.072554916560694 27.725210980731614 22.748252371934548 54-55 22.4604609103334 26.908907268195843 27.871934706542312 22.75869711492845 56-57 22.62076154679222 26.92577210235391 27.59461392030429 22.858852430549582 58-59 22.342014146035673 26.912957807788178 27.977667718451084 22.767360327725065 60-61 22.56432054052023 26.990323344337657 27.82993855955124 22.615417555590863 62-63 22.582170837263345 26.973116812498883 27.70500904363124 22.739703306606533 64-65 22.330583304540063 26.787373843832558 27.78731517075676 23.094727680870623 66-67 22.37836458514814 26.75087192255437 27.667354980507834 23.203408511789654 68-69 22.171056658733157 26.812250372437894 27.776459751896414 23.24023321693253 70-71 22.207573456267067 26.565011877271754 27.875341979767438 23.35207268669374 72-73 22.23830444569601 26.876440835426486 27.546183698933536 23.33907101994397 74-75 22.31897076753608 26.901421112961764 27.420634751778504 23.35897336772365 76-77 22.213222563285758 26.78840833064204 27.484967445952023 23.513401660120184 78-79 22.080860821580075 26.77263068007744 27.538566113388974 23.60794238495351 80-81 22.02621731594348 26.93630136279418 27.419762226663053 23.617719094599284 82-83 22.26734027152738 26.751564733364948 27.613903055115358 23.36719193999232 84-85 22.3082236086754 26.84275491289032 27.287222175137867 23.561799303296414 86-87 21.9793673404416 26.90208405544201 27.511380794933448 23.60716780918294 88-89 21.955518601619932 26.652680958325583 27.484832717538378 23.90696772251611 90-91 22.113908134325325 26.594781154948322 27.507250106185793 23.78406060454056 92-93 22.088278728576693 26.632680971067646 27.43401811437696 23.8450221859787 94-95 21.752861603957616 26.5175918717619 27.563751166636685 24.1657953576438 96-97 21.77342687882761 26.471544939199564 27.584356934175798 24.17067124779703 98-99 21.707527261019486 26.471845158785545 27.422753050601244 24.39787452959372 100 21.639175268934437 26.492980484301125 27.49067934220587 24.377164904558565 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1862.0 1 1396.0 2 1642.5 3 2211.5 4 1583.5 5 1200.5 6 1802.0 7 3409.0 8 5130.0 9 5884.0 10 5187.0 11 4137.5 12 4167.5 13 4623.5 14 5095.5 15 5661.5 16 6189.0 17 6848.0 18 7614.5 19 8283.0 20 9061.5 21 10067.5 22 11385.0 23 13312.5 24 15823.5 25 18955.0 26 23290.0 27 28859.5 28 34921.0 29 42778.5 30 52783.0 31 62580.5 32 74503.5 33 87988.5 34 100260.0 35 112838.0 36 127947.0 37 143568.5 38 152398.5 39 157330.5 40 161833.0 41 165154.5 42 170926.0 43 179956.5 44 189628.0 45 201186.0 46 211841.5 47 218619.5 48 225714.5 49 230881.0 50 229587.0 51 225025.5 52 218642.0 53 209866.0 54 199349.5 55 183522.5 56 165261.5 57 145465.0 58 125781.0 59 108756.5 60 90006.5 61 72434.0 62 57906.0 63 44599.5 64 33193.0 65 23996.5 66 17592.5 67 13514.0 68 10337.5 69 7703.5 70 5768.5 71 4336.0 72 3246.0 73 2489.0 74 1881.0 75 1278.0 76 858.5 77 539.0 78 301.5 79 181.5 80 109.0 81 64.0 82 42.5 83 33.0 84 18.5 85 11.0 86 10.0 87 7.0 88 3.0 89 2.0 90 2.5 91 2.0 92 1.5 93 1.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.027774181779666007 2 0.0011768721093078814 3 5.431717427574838E-5 4 0.016023566411345773 5 5.25066017998901E-4 6 9.052862379291397E-5 7 1.8105724758582793E-5 8 0.0034762991536478965 9 0.007224184178674534 10-11 9.777091369634707E-4 12-13 8.147576141362257E-5 14-15 1.4484579806866234E-4 16-17 4.435902565852784E-4 18-19 9.052862379291396E-6 20-21 5.431717427574838E-5 22-23 2.896915961373247E-4 24-25 5.431717427574838E-5 26-27 5.431717427574838E-5 28-29 0.0010682377607563848 30-31 0.0023537442186157627 32-33 0.008573060673188953 34-35 0.01608693644800081 36-37 0.018377310629961535 38-39 0.018848059473684686 40-41 0.004571695501542155 42-43 0.0052053958680925525 44-45 0.007821673095707766 46-47 0.010220681626219986 48-49 1.1911213146928863 50-51 0.0167296896769305 52-53 0.01787940319910051 54-55 0.008672642159361157 56-57 0.007857884545224932 58-59 0.00791220171950068 60-61 0.0052053958680925525 62-63 0.00803894179281076 64-65 0.01794277323575555 66-67 0.025510966184843154 68-69 0.0251488516896715 70-71 0.02216140710450534 72-73 0.020731054848577297 74-75 0.00790314885712139 76-77 0.01237526287249134 78-79 0.0070793383806058725 80-81 0.002245109870064266 82-83 0.012465791496284252 84-85 0.020115460206785483 86-87 0.019789557161130993 88-89 0.02762933598159734 90-91 0.017281914282067275 92-93 0.010781959093736053 94-95 0.005332135941402633 96-97 0.0030689203465797837 98-99 0.013778456541281504 100 0.027321538660701434 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 5523115.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.58700277437529 #Duplication Level Percentage of deduplicated Percentage of total 1 78.29427133517771 51.350865912779916 2 13.814255000242785 18.120711620537026 3 3.8575855967902704 7.590224317172208 4 1.5342329197195506 4.025029550487362 5 0.7872992642927058 2.581829951571466 6 0.4292902162395974 1.6893515162111183 7 0.27697404755346416 1.2716128337723307 8 0.20172995945834113 1.0584690728535069 9 0.1369095901938216 0.8081540704692682 >10 0.6245532984189841 7.037656200820557 >50 0.028365080358482692 1.2788410598483035 >100 0.013592883189772207 1.5936618624717112 >500 5.712052445895298E-4 0.2638762087101338 >1k 2.688022690149372E-4 0.3847953723647825 >5k 3.360028362676025E-5 0.2195808424624508 >10k+ 6.72005672535205E-5 0.7253396074677947 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17581 0.3183167469806441 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15333 0.27761507772335 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9964 0.18040544149451893 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 1.8105724758582793E-5 0.0 0.0 0.0 4 0.0 1.8105724758582793E-5 0.0 0.0 0.0 5 0.0 1.8105724758582793E-5 0.0 0.0 0.0 6 0.0 1.8105724758582793E-5 0.0 0.0 5.4317174275748375E-5 7 0.0 1.8105724758582793E-5 0.0 0.0 5.4317174275748375E-5 8 0.0 1.8105724758582793E-5 0.0 0.0 7.242289903433117E-5 9 0.0 1.8105724758582793E-5 0.0 0.0 9.052862379291397E-5 10-11 0.0 1.8105724758582793E-5 0.0 1.8105724758582793E-5 9.052862379291397E-5 12-13 0.0 4.526431189645698E-5 0.0 1.8105724758582793E-5 9.052862379291397E-5 14-15 0.0 5.4317174275748375E-5 0.0 3.6211449517165586E-5 1.1768721093078816E-4 16-17 0.0 5.4317174275748375E-5 0.0 7.242289903433117E-5 1.4484579806866234E-4 18-19 0.0 5.4317174275748375E-5 0.0 7.242289903433117E-5 1.4484579806866234E-4 20-21 0.0 6.337003665503977E-5 0.0 8.147576141362257E-5 1.4484579806866234E-4 22-23 0.0 7.242289903433117E-5 0.0 2.172686971029935E-4 1.7200438520653652E-4 24-25 0.0 7.242289903433117E-5 0.0 8.328633388948084E-4 1.991629723444107E-4 26-27 0.0 7.242289903433117E-5 0.0 0.0018286782006168621 1.991629723444107E-4 28-29 0.0 7.242289903433117E-5 0.0 0.004924757134334519 1.991629723444107E-4 30-31 0.0 7.242289903433117E-5 0.0 0.016105042172759394 1.991629723444107E-4 32-33 0.0 7.242289903433117E-5 0.0 0.04138063393574097 2.172686971029935E-4 34-35 0.0 9.052862379291397E-5 0.0 0.08014499064386674 2.534801466201591E-4 36-37 0.0 9.958148617220537E-5 0.0 0.14050947698898175 2.534801466201591E-4 38-39 9.052862379291396E-6 1.0863434855149675E-4 0.0 0.22766138311442002 2.7158587137874186E-4 40-41 1.8105724758582793E-5 1.0863434855149675E-4 0.0 0.32463564492138947 2.896915961373247E-4 42-43 1.8105724758582793E-5 1.0863434855149675E-4 0.0 0.41910226384929516 3.0779732089590746E-4 44-45 1.8105724758582793E-5 1.0863434855149675E-4 0.0 0.5372330650366686 3.4400877041307305E-4 46-47 1.8105724758582793E-5 1.0863434855149675E-4 0.0 0.6759953395864471 3.9832594468882146E-4 48-49 1.8105724758582793E-5 1.0863434855149675E-4 0.0 0.8249511371753078 4.1643166944740423E-4 50-51 1.8105724758582793E-5 1.0863434855149675E-4 0.0 0.980198674117776 4.1643166944740423E-4 52-53 1.8105724758582793E-5 1.0863434855149675E-4 0.0 1.1438563202106058 4.526431189645698E-4 54-55 2.7158587137874188E-5 1.0863434855149675E-4 0.0 1.3267150874099127 4.888545684817354E-4 56-57 3.6211449517165586E-5 1.0863434855149675E-4 0.0 1.5372303491779546 4.888545684817354E-4 58-59 3.6211449517165586E-5 1.0863434855149675E-4 0.0 1.772351290892911 5.160131556196096E-4 60-61 3.6211449517165586E-5 1.0863434855149675E-4 0.0 2.0094910209184493 5.25066017998901E-4 62-63 3.6211449517165586E-5 1.1768721093078815E-4 0.0 2.256136256442243 5.431717427574837E-4 64-65 3.6211449517165586E-5 1.2674007331007955E-4 0.0 2.507670037650855 5.431717427574837E-4 66-67 3.6211449517165586E-5 1.2674007331007955E-4 0.0 2.79160401331495 5.431717427574837E-4 68-69 3.6211449517165586E-5 1.2674007331007955E-4 0.0 3.10690615712329 5.431717427574837E-4 70-71 3.6211449517165586E-5 1.6295152282724514E-4 0.0 3.4350271540607067 5.431717427574837E-4 72-73 3.6211449517165586E-5 1.991629723444107E-4 0.0 3.750483196529495 6.518060913089806E-4 74-75 3.6211449517165586E-5 1.991629723444107E-4 0.0 4.0680304502079 6.699118160675633E-4 76-77 3.6211449517165586E-5 1.991629723444107E-4 0.0 4.420467435496093 6.789646784468547E-4 78-79 4.526431189645698E-5 1.991629723444107E-4 0.0 4.811876993327135 6.880175408261461E-4 80-81 5.4317174275748375E-5 1.991629723444107E-4 0.0 5.210474523887335 7.423347151018945E-4 82-83 5.4317174275748375E-5 1.991629723444107E-4 0.0 5.6184779784596195 8.328633388948085E-4 84-85 5.4317174275748375E-5 1.991629723444107E-4 0.0 6.026888811838971 8.419162012740999E-4 86-87 5.4317174275748375E-5 1.991629723444107E-4 0.0 6.446262661559645 8.69074788411974E-4 88 9.052862379291397E-5 1.991629723444107E-4 0.0 6.785609207847383 9.052862379291397E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8855 0.0 51.514896 1 GTATCAA 15960 0.0 41.255318 1 ATCAACG 20055 0.0 32.192924 3 CAACGCA 20445 0.0 31.734589 5 TCAACGC 20765 0.0 31.16025 4 TATCAAC 20970 0.0 31.141436 2 AACGCAG 21855 0.0 30.073582 6 TACCTGG 4660 0.0 30.000479 2 ACGCAGA 25125 0.0 25.898119 7 CGCAGAG 25705 0.0 25.222506 8 GTGGTAT 4395 0.0 24.986885 1 GTACCTG 5765 0.0 24.910202 1 GTACATG 19370 0.0 24.32536 1 ACCTGGG 5805 0.0 24.248434 3 TACATGG 19925 0.0 23.215414 2 TGGTATC 4460 0.0 22.510063 2 GCAGAGT 28295 0.0 22.46629 9 ACATGGG 20115 0.0 22.346533 3 ATAACGC 580 0.0 21.842405 3 CATGGGG 14125 0.0 20.728352 4 >>END_MODULE