FastQCFastQC Report
Mon 6 Jun 2016
ERR1211346_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211346_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5269249
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT239350.4542393043107281No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT184560.35025864217082925No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121710.230981682588923No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69400.13170757350810333No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA93800.054.0096131
GTATCAA169550.042.0035741
TACCTGG46700.038.5758322
ATCAACG213650.032.6162723
CAACGCA214500.032.4657335
TATCAAC219100.032.073152
GTACCTG57350.031.6600841
TCAACGC221000.031.5740474
AACGCAG226150.031.1886566
ACCTGGG58750.030.0729483
GTACATG208700.028.128741
ACGCAGA253700.027.3580387
TACATGG211800.027.1158432
CGCAGAG259950.026.537328
ACATGGG214200.025.9953063
GTGGTAT42700.025.6676561
TGGTATC42750.024.205832
GCAGAGT282400.024.1140359
CATGGGG138050.023.6221354
TAACGCA5000.022.5547124
Produced by FastQC (version 0.11.5)