##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211346_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5269249 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.334057282166775 31.0 31.0 34.0 28.0 34.0 2 31.333036453581904 31.0 31.0 34.0 28.0 34.0 3 31.385956518661388 31.0 31.0 34.0 28.0 34.0 4 34.99031797510423 37.0 35.0 37.0 32.0 37.0 5 35.04125179888064 37.0 35.0 37.0 32.0 37.0 6 35.10907569560672 37.0 35.0 37.0 32.0 37.0 7 35.043412258559044 37.0 35.0 37.0 32.0 37.0 8 35.04415012461928 37.0 35.0 37.0 32.0 37.0 9 36.59553306363013 39.0 37.0 39.0 32.0 39.0 10-11 36.52153039266127 39.0 37.0 39.0 32.0 39.0 12-13 36.436313694797875 39.0 37.0 39.0 32.0 39.0 14-15 37.553834142208885 40.0 37.0 41.0 32.0 41.0 16-17 37.556953562073076 40.0 37.0 41.0 32.0 41.0 18-19 37.568197479375144 40.0 37.0 41.0 32.0 41.0 20-21 37.53260455142659 40.0 37.0 41.0 31.5 41.0 22-23 37.40223018498462 40.0 37.0 41.0 31.0 41.0 24-25 37.28759743561179 40.0 37.0 41.0 31.0 41.0 26-27 37.14015839828408 40.0 36.0 41.0 30.5 41.0 28-29 36.99435811441061 39.5 36.0 41.0 30.0 41.0 30-31 36.79525526313142 39.0 36.0 41.0 30.0 41.0 32-33 36.6478731599133 39.0 35.0 41.0 30.0 41.0 34-35 36.48448640403974 39.0 35.0 41.0 29.5 41.0 36-37 36.371163044297205 39.0 35.0 41.0 29.0 41.0 38-39 36.212367075459895 39.0 35.0 40.5 28.5 41.0 40-41 36.04277203449675 39.0 35.0 40.0 28.0 41.0 42-43 35.88454341406147 39.0 35.0 40.0 27.0 41.0 44-45 35.73042277941316 38.0 35.0 40.0 26.5 41.0 46-47 35.53939176152048 38.0 34.0 40.0 26.0 41.0 48-49 35.28506054657884 38.0 34.0 40.0 24.5 41.0 50-51 34.61195656155175 37.5 33.5 39.5 24.0 40.5 52-53 34.854211956960086 38.0 33.5 40.0 24.5 40.5 54-55 35.216006683305345 38.0 34.0 40.0 25.0 41.0 56-57 35.11761752006785 38.0 34.0 40.0 25.0 41.0 58-59 34.90019270298291 38.0 34.0 40.0 25.0 41.0 60-61 34.637135197065085 37.5 33.0 40.0 24.0 41.0 62-63 34.36772412918805 37.0 33.0 40.0 24.0 41.0 64-65 34.1000964274036 36.5 33.0 40.0 23.0 41.0 66-67 33.8820585248486 36.0 33.0 39.0 23.0 41.0 68-69 33.54737677038986 35.5 32.0 39.0 22.5 41.0 70-71 33.2068362113842 35.0 32.0 39.0 22.0 40.5 72-73 32.84350236627648 35.0 32.0 38.0 22.0 40.0 74-75 32.47562147850671 35.0 31.0 37.0 20.5 39.5 76-77 32.12247399961551 35.0 31.0 37.0 20.0 39.0 78-79 31.75497798642653 35.0 31.0 36.0 20.0 39.0 80-81 31.416136720811636 35.0 30.0 36.0 19.5 37.5 82-83 31.104395142457683 34.0 30.0 35.0 18.5 37.0 84-85 30.794416718587414 34.0 30.0 35.0 18.0 37.0 86-87 30.535614847580746 34.0 29.0 35.0 18.0 36.0 88-89 30.270179109015345 34.0 29.0 35.0 17.5 36.0 90-91 30.00579987774349 34.0 29.0 35.0 16.5 36.0 92-93 29.768772267167485 34.0 29.0 35.0 15.0 35.0 94-95 29.54130038265415 34.0 28.5 35.0 13.5 35.0 96-97 29.336813557301998 34.0 27.5 35.0 11.5 35.0 98-99 29.094408614965815 34.0 27.0 35.0 10.0 35.0 100 27.456142232033447 32.0 24.0 34.0 8.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.3312772568603286 1101 2 -0.40864210451966443 1101 3 -0.4187732300494069 1101 4 -0.27242890269203457 1101 5 -0.23516357772005847 1101 6 -0.30879961693401725 1101 7 -0.35405387832997093 1101 8 -0.43188887243454843 1101 9 -0.4549278853044072 1101 10-11 -0.5565426772623212 1101 12-13 -0.47800499579885525 1101 14-15 -0.4890479422287939 1101 16-17 -0.5098271719815273 1101 18-19 -0.6104175871993718 1101 20-21 -0.6450746442119737 1101 22-23 -0.6588276793824335 1101 24-25 -0.6023844328681065 1101 26-27 -0.6922298875776463 1101 28-29 -0.6577018668471979 1101 30-31 -0.785573148489398 1101 32-33 -0.7461690925197288 1101 34-35 -0.817213564056118 1101 36-37 -0.885331013557817 1101 38-39 -0.8761239930026363 1101 40-41 -0.8701880600473544 1101 42-43 -0.89565281696418 1101 44-45 -1.0149436088058934 1101 46-47 -0.8911166412677645 1101 48-49 -0.8666636658538422 1101 50-51 -0.807844629517966 1101 52-53 -0.6604741316082183 1101 54-55 -0.6201463737062483 1101 56-57 -0.6889708006021777 1101 58-59 -0.7346731469787997 1101 60-61 -0.6439012590059718 1101 62-63 -0.6271774910123256 1101 64-65 -0.6316398481756309 1101 66-67 -0.6999972977382996 1101 68-69 -0.6826608362892799 1101 70-71 -0.6076338081950112 1101 72-73 -0.6970986642950763 1101 74-75 -0.8107035900761375 1101 76-77 -0.8294796519127985 1101 78-79 -0.8953309675199641 1101 80-81 -0.8267878427838973 1101 82-83 -0.9058834550707644 1101 84-85 -0.7866208112091435 1101 86-87 -0.8119000270571668 1101 88-89 -0.8847931877973743 1101 90-91 -0.9650882115084194 1101 92-93 -0.9111911972479554 1101 94-95 -0.9921456921615395 1101 96-97 -1.0870294800448868 1101 98-99 -0.9875851474261346 1101 100 -1.1199015848915082 1102 1 -0.4037282887614211 1102 2 -0.46514792486872025 1102 3 -0.3963772000364365 1102 4 -0.29014484667219875 1102 5 -0.3081293287831528 1102 6 -0.2622758183558602 1102 7 -0.28070229312629635 1102 8 -0.28863152927202407 1102 9 -0.3924785773430912 1102 10-11 -0.4233868002695189 1102 12-13 -0.46437930417063455 1102 14-15 -0.6162880377843152 1102 16-17 -0.6229030499594188 1102 18-19 -0.5926343274755368 1102 20-21 -0.6038780247757884 1102 22-23 -0.7120424166047528 1102 24-25 -0.6444688812894697 1102 26-27 -0.6437096025529385 1102 28-29 -0.7826398871075995 1102 30-31 -0.755301997390184 1102 32-33 -0.7084187107902054 1102 34-35 -0.6322245644337485 1102 36-37 -0.7386805874984148 1102 38-39 -0.8633500097166404 1102 40-41 -0.9657366012659381 1102 42-43 -0.8823768388335935 1102 44-45 -0.9585956110388096 1102 46-47 -0.9654572343030523 1102 48-49 -0.9643808822657505 1102 50-51 -1.0048232198277773 1102 52-53 -0.8545142255218892 1102 54-55 -0.7282632568231264 1102 56-57 -0.6976329822890079 1102 58-59 -0.8356348590972758 1102 60-61 -0.8248102678226914 1102 62-63 -0.8444314601784342 1102 64-65 -0.9062944445274468 1102 66-67 -0.7839626055025946 1102 68-69 -0.8098323847155697 1102 70-71 -0.8800862767125821 1102 72-73 -0.8445412817282154 1102 74-75 -0.7735496094171026 1102 76-77 -0.7796473255792051 1102 78-79 -0.7817110223576655 1102 80-81 -0.849542000762451 1102 82-83 -0.8380932174954481 1102 84-85 -0.8468948765875091 1102 86-87 -0.8972032352269999 1102 88-89 -0.9785391857943182 1102 90-91 -0.99130317858938 1102 92-93 -0.9815624189466128 1102 94-95 -1.0283139486837811 1102 96-97 -1.015620610785195 1102 98-99 -0.8647031305807786 1102 100 -1.0993041987125807 1103 1 -0.512324454500444 1103 2 -0.46603640147148084 1103 3 -0.5024575958311175 1103 4 -0.4036039272741405 1103 5 -0.33818127683510113 1103 6 -0.4878763130992141 1103 7 -0.5273852450949619 1103 8 -0.45135199927820935 1103 9 -0.5627381114597654 1103 10-11 -0.6011459221000734 1103 12-13 -0.6311726457496931 1103 14-15 -0.7402308330842544 1103 16-17 -0.7286708297986308 1103 18-19 -0.6774500355769604 1103 20-21 -0.8163066992758914 1103 22-23 -0.7716025073076977 1103 24-25 -0.8877811880303454 1103 26-27 -0.7235371638805006 1103 28-29 -0.7584098314489722 1103 30-31 -0.8075062851658927 1103 32-33 -0.8320220915364516 1103 34-35 -1.002237654518261 1103 36-37 -1.0176289486610628 1103 38-39 -1.0153231080468856 1103 40-41 -1.0659830452980898 1103 42-43 -0.9469851670133522 1103 44-45 -1.0499121437124828 1103 46-47 -0.9671520394978543 1103 48-49 -1.0254768418617104 1103 50-51 -1.1569380415140316 1103 52-53 -0.9785788513722267 1103 54-55 -0.8834241514840215 1103 56-57 -0.9487667691370802 1103 58-59 -0.9649455166936463 1103 60-61 -1.0618260377670339 1103 62-63 -1.0811283268038707 1103 64-65 -1.0325075964548844 1103 66-67 -1.018686579940855 1103 68-69 -1.0044228815203482 1103 70-71 -1.0375807108498734 1103 72-73 -1.1772967960551597 1103 74-75 -1.0531500010889232 1103 76-77 -0.9415356991104353 1103 78-79 -0.9096529930853485 1103 80-81 -0.9773394652027747 1103 82-83 -1.1151887648052785 1103 84-85 -1.0715524729593895 1103 86-87 -1.150757450852641 1103 88-89 -1.2528900412880333 1103 90-91 -1.1181958811249402 1103 92-93 -1.165786084170275 1103 94-95 -1.3634524768223066 1103 96-97 -1.374971466278911 1103 98-99 -1.382682000916791 1103 100 -1.4141277402504073 1104 1 -0.3225908894845162 1104 2 -0.3939695996566357 1104 3 -0.48423054688817047 1104 4 -0.31925601724421426 1104 5 -0.2272933532903565 1104 6 -0.3641103125200047 1104 7 -0.2827326724528163 1104 8 -0.262893846946902 1104 9 -0.3532308231114669 1104 10-11 -0.40250242369288713 1104 12-13 -0.4640239827513355 1104 14-15 -0.5476368568317156 1104 16-17 -0.5660293586141592 1104 18-19 -0.6775302556754212 1104 20-21 -0.7188396657784466 1104 22-23 -0.5062985204363812 1104 24-25 -0.6266679531621122 1104 26-27 -0.615012788940831 1104 28-29 -0.6073802919181261 1104 30-31 -0.7878458932361738 1104 32-33 -0.8168637750966425 1104 34-35 -0.758882697476075 1104 36-37 -0.7853677265353767 1104 38-39 -0.7321050368044837 1104 40-41 -0.8050435723760003 1104 42-43 -0.8111868274052796 1104 44-45 -0.8042778160746806 1104 46-47 -0.7993770612181024 1104 48-49 -0.8302238251612621 1104 50-51 -0.6933212831396673 1104 52-53 -0.5710129666343207 1104 54-55 -0.6068210044621054 1104 56-57 -0.6546867421074438 1104 58-59 -0.7753013425455606 1104 60-61 -0.6484889395915374 1104 62-63 -0.6969275352216684 1104 64-65 -0.7146110813472433 1104 66-67 -0.7940617126756777 1104 68-69 -0.679076419506643 1104 70-71 -0.6530516057238955 1104 72-73 -0.6589264900410612 1104 74-75 -0.7745095917830014 1104 76-77 -0.7125032635471555 1104 78-79 -0.7458769529270342 1104 80-81 -0.7334286630017921 1104 82-83 -0.7504815536845157 1104 84-85 -0.8286476558921088 1104 86-87 -0.8078154680357699 1104 88-89 -0.7111549317214738 1104 90-91 -0.6410145894944748 1104 92-93 -0.7111383382488548 1104 94-95 -0.7092181877982746 1104 96-97 -0.7630061221337208 1104 98-99 -0.7789338592042725 1104 100 -0.9042034232313902 1105 1 -0.4984490756324682 1105 2 -0.5524850281484888 1105 3 -0.5372956870413823 1105 4 -0.46259478745129456 1105 5 -0.4259317526227946 1105 6 -0.41397372622390094 1105 7 -0.4749975192426561 1105 8 -0.4763584136011829 1105 9 -0.6829621663456962 1105 10-11 -0.7321786280985236 1105 12-13 -0.7304190920267999 1105 14-15 -0.7407606843565446 1105 16-17 -0.8564970791818709 1105 18-19 -0.8490672203235547 1105 20-21 -0.908441658510867 1105 22-23 -0.9140008062198532 1105 24-25 -0.8561680210464857 1105 26-27 -0.8999059991992127 1105 28-29 -0.9161144201569442 1105 30-31 -1.0068607881146079 1105 32-33 -1.0305025078800512 1105 34-35 -1.0712349525140823 1105 36-37 -0.9915404873937064 1105 38-39 -0.9204962947671191 1105 40-41 -1.055658734309155 1105 42-43 -1.0216088835613633 1105 44-45 -1.1303178202708324 1105 46-47 -1.0790021135092687 1105 48-49 -1.0933469811911536 1105 50-51 -1.1100982351185067 1105 52-53 -0.9513232652722792 1105 54-55 -0.8539880950003891 1105 56-57 -0.9080487408952678 1105 58-59 -0.9143658545616447 1105 60-61 -0.9022777188835533 1105 62-63 -0.9705665683865377 1105 64-65 -0.9354237638381733 1105 66-67 -0.9054448562971444 1105 68-69 -0.8407153558513087 1105 70-71 -0.8672493511543777 1105 72-73 -0.838631304172921 1105 74-75 -0.8617307675182815 1105 76-77 -0.8193537015069978 1105 78-79 -0.8017026629709285 1105 80-81 -0.8223281167852114 1105 82-83 -0.9697326430036313 1105 84-85 -0.9814904678373289 1105 86-87 -0.9385292607255344 1105 88-89 -0.9900153202993351 1105 90-91 -1.055685977692221 1105 92-93 -1.017630284259564 1105 94-95 -1.0819159292787823 1105 96-97 -1.1197167495194371 1105 98-99 -1.176080699255646 1105 100 -1.0793147894268813 1106 1 -0.3688233619509269 1106 2 -0.4511951148475788 1106 3 -0.48844815056432367 1106 4 -0.31393756605142187 1106 5 -0.3677799234834396 1106 6 -0.3029565978669524 1106 7 -0.44370126210584004 1106 8 -0.365764207637973 1106 9 -0.4093313775954286 1106 10-11 -0.5961033479124396 1106 12-13 -0.6292349093243317 1106 14-15 -0.6816572798312706 1106 16-17 -0.7331094708080101 1106 18-19 -0.7367813247853903 1106 20-21 -0.7494228384643478 1106 22-23 -0.6975702712230216 1106 24-25 -0.6810781500378198 1106 26-27 -0.7891003320391405 1106 28-29 -0.7606904404572177 1106 30-31 -0.7842978784085375 1106 32-33 -0.8132261280957422 1106 34-35 -0.7628187968786406 1106 36-37 -0.8793603224539694 1106 38-39 -0.8564235521154018 1106 40-41 -0.8012709144740953 1106 42-43 -0.8592066843649135 1106 44-45 -1.0040754857392216 1106 46-47 -0.9964265161313932 1106 48-49 -0.9231207177104963 1106 50-51 -0.7498740862498252 1106 52-53 -0.7887096048789601 1106 54-55 -0.9202811802192485 1106 56-57 -0.855923555866724 1106 58-59 -0.7057593838490206 1106 60-61 -0.6953629377458839 1106 62-63 -0.6069590403679328 1106 64-65 -0.7552588241046152 1106 66-67 -0.8508878675045892 1106 68-69 -0.8122738248722428 1106 70-71 -0.7966213113996687 1106 72-73 -0.7131290838303528 1106 74-75 -0.7402463099162517 1106 76-77 -0.7213414367575766 1106 78-79 -0.7669172960857473 1106 80-81 -0.8417825468972815 1106 82-83 -0.826044759730209 1106 84-85 -0.6884052489749912 1106 86-87 -0.7445136125969647 1106 88-89 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0.7447325297841516 2311 14-15 0.9124445197930555 2311 16-17 0.8295072375195005 2311 18-19 0.790775805899699 2311 20-21 0.8160953998916796 2311 22-23 0.952316421392986 2311 24-25 0.8976346643040785 2311 26-27 0.949301417371764 2311 28-29 0.958593425880018 2311 30-31 1.0126562193581705 2311 32-33 0.9709999859155971 2311 34-35 1.0171155847144533 2311 36-37 1.0781213227830193 2311 38-39 1.0843935043309614 2311 40-41 1.0558334585021285 2311 42-43 1.134663403381147 2311 44-45 1.1942633901275457 2311 46-47 1.148932651055894 2311 48-49 1.1575061476424438 2311 50-51 1.1776681836036005 2311 52-53 1.048104101939387 2311 54-55 1.019633756586913 2311 56-57 1.0110504585140205 2311 58-59 0.93051883365019 2311 60-61 1.0282619101157024 2311 62-63 1.0375748943325718 2311 64-65 0.9845318534184457 2311 66-67 1.0931205137978353 2311 68-69 1.0356234449363981 2311 70-71 1.0470316670002902 2311 72-73 0.9923506885630076 2311 74-75 0.9993480083211352 2311 76-77 1.0291407395432017 2311 78-79 1.0183299964188102 2311 80-81 1.0832392535811515 2311 82-83 1.0000262189080757 2311 84-85 1.1190942878078936 2311 86-87 1.202274398586372 2311 88-89 1.1282040593272384 2311 90-91 1.1154078105145828 2311 92-93 1.0720883277008753 2311 94-95 1.1258222245892 2311 96-97 1.1934027646295213 2311 98-99 1.2563567251056469 2311 100 1.2557390711864365 2312 1 0.5363589782471507 2312 2 0.5585327138311484 2312 3 0.5620307700133651 2312 4 0.4288611881717941 2312 5 0.4526289762682367 2312 6 0.5207534896843882 2312 7 0.5092872968650184 2312 8 0.5302825861374529 2312 9 0.6313186711006438 2312 10-11 0.6854827521973093 2312 12-13 0.7165194029006017 2312 14-15 0.8965084328623334 2312 16-17 0.877396288262517 2312 18-19 0.889354682570179 2312 20-21 0.9138885109270589 2312 22-23 0.8810494711256709 2312 24-25 0.8882880257909278 2312 26-27 1.0225867904785062 2312 28-29 0.9588245712593988 2312 30-31 1.036352618232101 2312 32-33 0.9642830117290018 2312 34-35 1.0175957952461872 2312 36-37 1.0427514586687252 2312 38-39 1.077285629660679 2312 40-41 1.1217861524019455 2312 42-43 1.1749465147893616 2312 44-45 1.0590060858180266 2312 46-47 1.19524147976702 2312 48-49 1.1295243761869003 2312 50-51 1.1047174835903135 2312 52-53 1.0422027596761865 2312 54-55 0.9846234957117801 2312 56-57 0.9053808655199873 2312 58-59 0.8797254668806076 2312 60-61 0.9477061240901321 2312 62-63 0.949617244335542 2312 64-65 0.9696489765295127 2312 66-67 0.9680163953220173 2312 68-69 0.8795094809565924 2312 70-71 0.8705732469125067 2312 72-73 0.9181927341005007 2312 74-75 0.9249799002133159 2312 76-77 0.9929407370226144 2312 78-79 0.9624504335720054 2312 80-81 0.9828100323521731 2312 82-83 1.0122228102390523 2312 84-85 1.0173203484352769 2312 86-87 0.9632305477860079 2312 88-89 1.0683143401044823 2312 90-91 1.0358422724566587 2312 92-93 1.0971908445559642 2312 94-95 1.0831439550117494 2312 96-97 1.1190936276720471 2312 98-99 1.1365525271789316 2312 100 1.2002240608494184 2313 1 0.38983766453577573 2313 2 0.38211316504093773 2313 3 0.4806973427359793 2313 4 0.3628388151331805 2313 5 0.3659265897677386 2313 6 0.3475307397171292 2313 7 0.395854688951097 2313 8 0.39607005274029916 2313 9 0.49609407831737684 2313 10-11 0.5181773585764873 2313 12-13 0.5695853990351551 2313 14-15 0.7282872776545375 2313 16-17 0.7567909541490252 2313 18-19 0.6400937652846963 2313 20-21 0.6813319369668633 2313 22-23 0.6299253901445567 2313 24-25 0.6241281784927111 2313 26-27 0.742768139028918 2313 28-29 0.7259439263298688 2313 30-31 0.7956846621241382 2313 32-33 0.7361378467647057 2313 34-35 0.7149021784693446 2313 36-37 0.7977367765663175 2313 38-39 0.8448217778634373 2313 40-41 0.82497131883148 2313 42-43 0.7660375445445453 2313 44-45 0.843163437373633 2313 46-47 0.8268328017339925 2313 48-49 0.845633152287526 2313 50-51 0.7033996532894449 2313 52-53 0.6319485141934322 2313 54-55 0.5968613652438108 2313 56-57 0.6121342467663311 2313 58-59 0.6409191181662024 2313 60-61 0.6395609097357564 2313 62-63 0.6770270372725093 2313 64-65 0.7045481452609863 2313 66-67 0.6713948518978796 2313 68-69 0.7024063296918186 2313 70-71 0.6731151563912263 2313 72-73 0.6282572702179081 2313 74-75 0.597717186826614 2313 76-77 0.6211794756143121 2313 78-79 0.6270011210575142 2313 80-81 0.7530396635669696 2313 82-83 0.7064684874685341 2313 84-85 0.7790665898564164 2313 86-87 0.7988518252496917 2313 88-89 0.7171089481274251 2313 90-91 0.8864072782814745 2313 92-93 0.8257419728479682 2313 94-95 0.9228438372035406 2313 96-97 0.9880696485554061 2313 98-99 1.0270503537451923 2313 100 0.9868155074491831 2314 1 0.34321328336570645 2314 2 0.26937875069158324 2314 3 0.35825268853920633 2314 4 0.28986695862138134 2314 5 0.2516763241912017 2314 6 0.29330307539768796 2314 7 0.29810146290873973 2314 8 0.27826399387979706 2314 9 0.445798272261996 2314 10-11 0.4676896562621238 2314 12-13 0.45801643557024363 2314 14-15 0.5680101333472223 2314 16-17 0.5973627387282008 2314 18-19 0.6127428230931287 2314 20-21 0.6382153590235902 2314 22-23 0.6723238699996941 2314 24-25 0.6667764343771836 2314 26-27 0.6709739899930653 2314 28-29 0.6663913231372973 2314 30-31 0.6566789475331305 2314 32-33 0.7430776875241989 2314 34-35 0.7410291119274675 2314 36-37 0.7505626271353876 2314 38-39 0.7159347495541368 2314 40-41 0.7520126784564525 2314 42-43 0.718077194899557 2314 44-45 0.8021428773123915 2314 46-47 0.6315689354861078 2314 48-49 0.6259705959545485 2314 50-51 0.6904020625412812 2314 52-53 0.6203200154227275 2314 54-55 0.6366929463707294 2314 56-57 0.7118507919775965 2314 58-59 0.6318858566938488 2314 60-61 0.5228975029641987 2314 62-63 0.6525238718989996 2314 64-65 0.6473751631488511 2314 66-67 0.7185774069453217 2314 68-69 0.7888955562246451 2314 70-71 0.7009131955003554 2314 72-73 0.7681833550388504 2314 74-75 0.7831320885696869 2314 76-77 0.6755139053368637 2314 78-79 0.7205821291439953 2314 80-81 0.7920513836319536 2314 82-83 0.7803224603390433 2314 84-85 0.8110883936930229 2314 86-87 0.830651500754513 2314 88-89 0.681482490961983 2314 90-91 0.6773221860121126 2314 92-93 0.9198520741933471 2314 94-95 0.9524741855681107 2314 96-97 0.8819732276131873 2314 98-99 0.8352331868816556 2314 100 0.9587575851006029 2315 1 0.32934221216622106 2315 2 0.2930670857514457 2315 3 0.34492952826883894 2315 4 0.2647915266295726 2315 5 0.2317475668751925 2315 6 0.2589017470447956 2315 7 0.19864849766940296 2315 8 0.31259420995516507 2315 9 0.17966016610623825 2315 10-11 0.30340913931458147 2315 12-13 0.31294051251657606 2315 14-15 0.4542943681016851 2315 16-17 0.3198783019252147 2315 18-19 0.47297923168267886 2315 20-21 0.36520636016220465 2315 22-23 0.36515917559501787 2315 24-25 0.5387120351801613 2315 26-27 0.5788291357100732 2315 28-29 0.514566661562462 2315 30-31 0.5968889569631415 2315 32-33 0.6123058768385334 2315 34-35 0.637523816609793 2315 36-37 0.6411865955998408 2315 38-39 0.7671838805172797 2315 40-41 0.7653386404282969 2315 42-43 0.7385128564230499 2315 44-45 0.7743553982894937 2315 46-47 0.7565874776558914 2315 48-49 0.67400423960094 2315 50-51 0.5631571864967029 2315 52-53 0.2840852739983788 2315 54-55 0.5647170316304795 2315 56-57 0.5772726915434205 2315 58-59 0.6596310045544129 2315 60-61 0.6548823485099646 2315 62-63 0.6621417564941723 2315 64-65 0.6533271487095931 2315 66-67 0.5791540949144363 2315 68-69 0.6067154779318642 2315 70-71 0.5515755788578787 2315 72-73 0.594860270159721 2315 74-75 0.2606410479016219 2315 76-77 0.4928514249895244 2315 78-79 0.6757747211600282 2315 80-81 0.67615502865576 2315 82-83 0.6521377917385607 2315 84-85 0.7209859288477531 2315 86-87 0.5800991071030275 2315 88-89 0.5534378564953926 2315 90-91 0.7539436783837417 2315 92-93 0.836889618215718 2315 94-95 0.8324717078452046 2315 96-97 0.8969155166379359 2315 98-99 0.9654648295222863 2315 100 1.0369554166040338 2316 1 0.12784762562926844 2316 2 0.09201963353732268 2316 3 0.17425269236387209 2316 4 0.019356304258195678 2316 5 0.014217279574751274 2316 6 -0.14892176526721101 2316 7 -0.14304962782070163 2316 8 -0.05650613913908131 2316 9 -0.027620723869759445 2316 10-11 0.03169800279139423 2316 12-13 0.047657558061523275 2316 14-15 -0.11346316741136064 2316 16-17 0.04061485459908454 2316 18-19 0.14069312044584592 2316 20-21 0.17328006432750698 2316 22-23 0.06445729358843977 2316 24-25 0.06352128594841844 2316 26-27 -0.05567549050148557 2316 28-29 -0.011437447956105018 2316 30-31 -0.5976556827982691 2316 32-33 -0.41401600683885675 2316 34-35 -0.5625071595481614 2316 36-37 -0.2667774919949224 2316 38-39 -0.1345398034807488 2316 40-41 0.0634968961214426 2316 42-43 0.029247550876355888 2316 44-45 -0.17777800141075772 2316 46-47 -0.29998477133529633 2316 48-49 -0.0743039597374775 2316 50-51 0.05316208575901271 2316 52-53 -0.3324951126829703 2316 54-55 -0.24175119090379127 2316 56-57 -0.2640988123053063 2316 58-59 -0.6131789145352329 2316 60-61 -0.14835545876185563 2316 62-63 -0.19280949911064482 2316 64-65 -0.5567165857674325 2316 66-67 -0.8843211668265027 2316 68-69 -0.8101454262838459 2316 70-71 -0.5035168476771119 2316 72-73 -0.8249064339371941 2316 74-75 -0.5738418434098236 2316 76-77 -0.26630414934318836 2316 78-79 -0.2168615639659741 2316 80-81 -0.233576611497039 2316 82-83 0.01380762621935716 2316 84-85 0.01919796726586398 2316 86-87 0.12736427796859928 2316 88-89 0.18037716236627332 2316 90-91 -0.4352921727719945 2316 92-93 -0.5043971865640593 2316 94-95 -0.12744206832813987 2316 96-97 0.15098442402218737 2316 98-99 0.20152996972323578 2316 100 0.23368225678570198 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 147.0 1 355.0 2 233.0 3 221.0 4 215.0 5 187.0 6 236.0 7 623.0 8 1681.0 9 2804.0 10 4162.0 11 6764.0 12 10357.0 13 13483.0 14 15737.0 15 18485.0 16 22065.0 17 27041.0 18 32919.0 19 38647.0 20 46380.0 21 55058.0 22 61358.0 23 62766.0 24 62096.0 25 63489.0 26 68643.0 27 77544.0 28 90162.0 29 105219.0 30 127754.0 31 155748.0 32 194450.0 33 249610.0 34 339793.0 35 474773.0 36 688937.0 37 987034.0 38 929271.0 39 232535.0 40 267.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.362150222394845 18.615143882276808 12.757003145286255 23.265702750042095 2 15.922564028710276 20.085185420214 40.55448040427546 23.437770146800265 3 18.977504623384238 26.2653020588889 29.3769350307982 25.380258286928658 4 12.500721291460973 16.421984356327094 38.180576456135604 32.896717896076325 5 13.097204063007286 37.45637992064107 34.68677062595763 14.759645390394017 6 30.41033894031406 36.874037360293826 18.418620299963848 14.297003399428265 7 27.324058012400847 32.28684710986084 22.551405652381284 17.837689225357035 8 25.93788184511356 35.03949355899201 20.514665510325433 18.507959085569 9 26.733202570008118 15.818584490801385 20.877101458519853 36.571111480670645 10-11 25.032175105476824 26.173705510880303 28.14048299394506 20.653636389697816 12-13 25.45727910342806 24.039479419640077 28.428943043867207 22.07429843306466 14-15 22.857522537594296 25.316969105876268 26.46865381922939 25.356854537300045 16-17 21.88754269059384 28.09975621105542 27.281199849409397 22.73150124894134 18-19 21.651161773013218 27.142469441976658 29.625423045192566 21.580945739817558 20-21 22.667393112040905 26.433972504631186 29.43582858585332 21.462805797474587 22-23 22.529264039813583 26.320628175407375 29.139029026559193 22.011078758219853 24-25 22.071567752115055 26.411202701677468 29.573069670497098 21.94415987571038 26-27 21.99973548321738 26.631836408238073 29.304842109780544 22.063585998764008 28-29 21.794946893172 26.75490066981525 29.478279304025566 21.97187313298718 30-31 22.357443052267996 26.578934202265614 29.358790292239334 21.70483245322706 32-33 22.155232663117083 26.805143891851518 29.110434264360467 21.92918918067093 34-35 22.32425157440245 26.785200118591046 29.232189437327644 21.658358869678864 36-37 22.3640485635028 26.642507816945354 29.007298726828495 21.986144892723352 38-39 22.19255106344823 26.86276339614212 29.10916304010087 21.835522500308773 40-41 22.212304965855406 26.503357588889543 29.302258194403397 21.98207925085166 42-43 22.065075439981506 26.6627771350609 28.97425076275487 22.297896662202728 44-45 21.995226474702765 27.046716857866564 28.932874602957888 22.025182064472784 46-47 22.069509538075728 26.823037602077697 28.57947611533594 22.527976744510635 48-49 22.226678466853087 26.860520903980873 28.56897483364329 22.343825795522747 50-51 22.170590783642634 27.05960718986349 28.353046249074197 22.416755777419677 52-53 22.024141513638686 27.35521045252377 28.14307691402005 22.477571119817497 54-55 22.048539297254422 27.21461789793428 28.194508462121604 22.5423343426897 56-57 22.200338815145795 27.180273944093695 28.02372294669866 22.595664294061848 58-59 22.038457277039612 27.121284895467756 28.325155213073394 22.515102614419234 60-61 22.19521899528439 27.306376372325165 28.11008392519388 22.38832070719657 62-63 22.12056860745519 27.173020473844613 28.15771476943652 22.54869614926368 64-65 21.929938658111777 27.0299266005049 28.21778126741588 22.822353473967436 66-67 22.0547339799078 26.95489248258678 28.051214546359066 22.93915899114635 68-69 21.813800492864573 27.10444674741189 28.113048378298956 22.96870438142458 70-71 21.914369114334022 26.817615943537103 28.177243711955374 23.090771230173498 72-73 22.04585583132328 26.995309176217514 28.000165589763515 22.958669402695694 74-75 22.040559139544026 27.120178051825704 27.921688488483888 22.91757432014638 76-77 21.93259312354921 26.993007723309013 27.940634479092 23.133764674049775 78-79 21.832125967146272 26.948079076218644 28.025660044572664 23.19413491206242 80-81 21.786809433674726 27.18618627600387 27.85389454554259 23.17310974477881 82-83 22.068707083469334 26.912660522516685 28.10690380450066 22.911728589513324 84-85 22.111297394182767 26.960429943608073 27.771701265756445 23.156571396452723 86-87 21.736005579596117 27.126864984369575 28.090410572370022 23.046718863664285 88-89 21.932115378334014 26.71247860754792 28.108808486945 23.246597527173066 90-91 22.073450134514943 26.754144742864327 28.034059267097717 23.138345855523013 92-93 22.044343863215605 26.7619472495516 28.009042434795028 23.184666452437767 94-95 21.70355521668523 26.585310566641596 28.199691693003874 23.5114425236693 96-97 21.878047599287047 26.520084361821432 28.09990285665992 23.5019651822316 98-99 21.755014019158438 26.615887974369308 27.97083270496253 23.658265301509722 100 21.99947516646173 26.41326441710319 27.73454520095595 23.85271521547913 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2935.0 1 2069.5 2 3390.0 3 4683.0 4 2783.5 5 1995.5 6 2940.5 7 5225.0 8 8006.5 9 8907.5 10 7248.0 11 5679.5 12 5641.5 13 5948.0 14 6360.5 15 6811.5 16 7240.0 17 7827.5 18 8363.5 19 8851.5 20 9660.5 21 10844.0 22 12306.0 23 14329.0 24 17103.0 25 20659.5 26 25005.5 27 30367.5 28 36183.5 29 43753.0 30 54641.0 31 64874.5 32 76950.5 33 91726.5 34 105580.0 35 118727.5 36 133612.0 37 150093.0 38 158776.5 39 160942.0 40 160618.0 41 159877.0 42 161788.0 43 167653.5 44 174891.5 45 185026.0 46 194474.5 47 199085.5 48 203932.5 49 205941.5 50 203685.0 51 199129.0 52 192206.0 53 185241.5 54 178196.0 55 165731.0 56 149318.5 57 130551.5 58 113659.5 59 100367.5 60 85292.5 61 69478.0 62 56052.0 63 44288.0 64 33360.0 65 24356.5 66 18056.0 67 13885.5 68 10745.5 69 8207.0 70 6341.0 71 4996.0 72 3928.0 73 3020.0 74 2380.5 75 1762.0 76 1242.0 77 836.5 78 560.0 79 397.5 80 280.0 81 195.5 82 130.0 83 104.5 84 89.5 85 66.0 86 49.5 87 43.0 88 34.0 89 25.5 90 20.0 91 18.5 92 17.0 93 13.5 94 8.5 95 7.0 96 5.5 97 4.0 98 3.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02592399789799267 2 0.1121032617741162 3 0.01749774967931863 4 0.017478771642790082 5 0.01960431173398714 6 0.04708450862732052 7 0.047691805796233964 8 0.039113733285331555 9 0.03258528871951202 10-11 0.03753855625346231 12-13 0.03600133529465015 14-15 0.03153200769217777 16-17 0.037728336618747756 18-19 0.036694033627942046 20-21 0.047587426595326957 22-23 0.04751151444921278 24-25 0.06610999024718703 26-27 0.05774067613809862 28-29 0.05024435170932328 30-31 0.04849837234869713 32-33 0.04444656154985274 34-35 0.04572757901552953 36-37 0.04110642712082879 38-39 0.04637283225750007 40-41 0.05495090476840248 42-43 0.056488125727214636 44-45 0.059211473969060874 46-47 0.06117570074976529 48-49 0.06130854700546511 50-51 0.0543246295629605 52-53 0.06418371953953969 54-55 0.051079385316579275 56-57 0.056089586960115186 58-59 0.054267695453374856 60-61 0.04923851577331039 62-63 0.05588082855830119 64-65 0.05435309661775331 66-67 0.05269251842150561 68-69 0.05876549011064006 70-71 0.058167681959990884 72-73 0.06116621173150102 74-75 0.05109836335310782 76-77 0.05169617150375699 78-79 0.061507816389014834 80-81 0.06313043851220544 82-83 0.052683029403241335 84-85 0.05826257214263361 86-87 0.055377910590294746 88-89 0.0640129172107828 90-91 0.06951654780406089 92-93 0.07312237474448446 94-95 0.07671871266664376 96-97 0.0727617920504421 98-99 0.08181431547455814 100 0.12578642611119725 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 5269249.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.24921059734439 #Duplication Level Percentage of deduplicated Percentage of total 1 77.19278860425538 48.82382943026835 2 14.278566228185078 18.062160847892148 3 4.224615904151196 8.016108630436484 4 1.6854865935170031 4.264227860494301 5 0.8135409128133301 2.572791026204305 6 0.4587093866018819 1.7407803957696655 7 0.2808148321839811 1.243292152176378 8 0.19911241073475722 1.0074962239286076 9 0.13755358253009864 0.7830139958878861 >10 0.672341391692445 7.280271827627469 >50 0.035329666356161936 1.5250138512234348 >100 0.0194242804553729 2.2340493493897577 >500 0.0012415112572473842 0.5349834759287224 >1k 3.2863518411891675E-4 0.4915638172291097 >5k 3.6515020457655195E-5 0.1602825075911445 >10k+ 1.0954506137296558E-4 1.2601346079521654 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 23935 0.4542393043107281 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18456 0.35025864217082925 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12171 0.230981682588923 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6940 0.13170757350810333 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 1.897803652854515E-5 0.0 0.0 0.0 5 0.0 1.897803652854515E-5 0.0 0.0 0.0 6 0.0 1.897803652854515E-5 0.0 0.0 0.0 7 0.0 1.897803652854515E-5 0.0 0.0 0.0 8 0.0 1.897803652854515E-5 0.0 0.0 0.0 9 0.0 1.897803652854515E-5 0.0 0.0 0.0 10-11 0.0 1.897803652854515E-5 0.0 2.8467054792817724E-5 0.0 12-13 0.0 1.897803652854515E-5 0.0 3.79560730570903E-5 1.897803652854515E-5 14-15 0.0 3.79560730570903E-5 0.0 6.642312784990803E-5 5.693410958563545E-5 16-17 0.0 5.693410958563545E-5 0.0 8.540116437845318E-5 7.59121461141806E-5 18-19 0.0 5.693410958563545E-5 0.0 1.518242922283612E-4 9.489018264272575E-5 20-21 0.0 5.693410958563545E-5 0.0 2.4671447487108693E-4 1.0437920090699832E-4 22-23 0.0 5.693410958563545E-5 0.0 5.218960045349916E-4 1.138682191712709E-4 24-25 0.0 5.693410958563545E-5 0.0 0.0011481712099769815 1.138682191712709E-4 26-27 0.0 5.693410958563545E-5 0.0 0.002524078858296505 1.2335723743554347E-4 28-29 0.0 5.693410958563545E-5 0.0 0.00663282376672653 1.3284625569981605E-4 30-31 0.0 5.693410958563545E-5 0.0 0.02149262636857738 1.3284625569981605E-4 32-33 0.0 5.693410958563545E-5 0.0 0.055036305932780936 1.3284625569981605E-4 34-35 0.0 6.642312784990803E-5 0.0 0.10592591088407474 1.518242922283612E-4 36-37 0.0 9.489018264272574E-5 0.0 0.18305265133608223 1.518242922283612E-4 38-39 0.0 1.423352739640886E-4 0.0 0.3005931205756266 1.518242922283612E-4 40-41 0.0 1.8029134702117892E-4 0.0 0.427812388444729 1.518242922283612E-4 42-43 0.0 1.897803652854515E-4 0.0 0.5522513739623995 1.7080232875690636E-4 44-45 0.0 1.897803652854515E-4 0.0 0.7045596061222387 1.897803652854515E-4 46-47 0.0 1.897803652854515E-4 0.0 0.8819378245362859 2.0875840181399665E-4 48-49 0.0 1.897803652854515E-4 0.0 1.0711203816710881 2.0875840181399665E-4 50-51 0.0 1.897803652854515E-4 0.0 1.264421172732585 2.0875840181399665E-4 52-53 0.0 1.897803652854515E-4 0.0 1.4509657827899194 2.562034931353595E-4 54-55 0.0 1.897803652854515E-4 0.0 1.660805932686043 2.846705479281772E-4 56-57 0.0 1.897803652854515E-4 0.0 1.90654303867591 2.846705479281772E-4 58-59 0.0 1.897803652854515E-4 0.0 2.174180798819718 2.846705479281772E-4 60-61 0.0 2.0875840181399665E-4 0.0 2.4422645428219467 2.846705479281772E-4 62-63 0.0 2.277364383425418E-4 0.0 2.7153110433763903 3.036485844567224E-4 64-65 0.0 2.277364383425418E-4 0.0 2.9933772345926335 3.036485844567224E-4 66-67 0.0 2.846705479281772E-4 0.0 3.3062776118570216 3.036485844567224E-4 68-69 0.0 2.846705479281772E-4 0.0 3.651260359872915 3.036485844567224E-4 70-71 0.0 2.941595661924498E-4 0.0 4.016208002316839 3.036485844567224E-4 72-73 9.489018264272575E-6 3.036485844567224E-4 0.0 4.377170257089768 3.7007171230663043E-4 74-75 1.897803652854515E-5 3.036485844567224E-4 0.0 4.734128146155173 3.8904974883517554E-4 76-77 1.897803652854515E-5 3.2262662098526756E-4 0.0 5.119486666885546 4.080277853637207E-4 78-79 1.897803652854515E-5 3.2262662098526756E-4 0.0 5.543427535878452 4.175168036279933E-4 80-81 1.897803652854515E-5 3.3211563924954014E-4 0.0 5.9853785615369475 4.175168036279933E-4 82-83 1.897803652854515E-5 3.416046575138127E-4 0.0 6.4379857547062205 4.175168036279933E-4 84-85 1.897803652854515E-5 3.416046575138127E-4 0.0 6.873987165913017 4.3649484015653847E-4 86-87 1.897803652854515E-5 3.416046575138127E-4 0.0 7.329962960566108 4.554728766850836E-4 88 1.897803652854515E-5 3.416046575138127E-4 0.0 7.688951499540067 4.554728766850836E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9380 0.0 54.009613 1 GTATCAA 16955 0.0 42.003574 1 TACCTGG 4670 0.0 38.575832 2 ATCAACG 21365 0.0 32.616272 3 CAACGCA 21450 0.0 32.465733 5 TATCAAC 21910 0.0 32.07315 2 GTACCTG 5735 0.0 31.660084 1 TCAACGC 22100 0.0 31.574047 4 AACGCAG 22615 0.0 31.188656 6 ACCTGGG 5875 0.0 30.072948 3 GTACATG 20870 0.0 28.12874 1 ACGCAGA 25370 0.0 27.358038 7 TACATGG 21180 0.0 27.115843 2 CGCAGAG 25995 0.0 26.53732 8 ACATGGG 21420 0.0 25.995306 3 GTGGTAT 4270 0.0 25.667656 1 TGGTATC 4275 0.0 24.20583 2 GCAGAGT 28240 0.0 24.114035 9 CATGGGG 13805 0.0 23.622135 4 TAACGCA 500 0.0 22.554712 4 >>END_MODULE