FastQCFastQC Report
Mon 6 Jun 2016
ERR1211346_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211346_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5269249
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT209320.3972482606155071No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT172480.32733317404434675No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117180.22238463204149206No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54930.1042463546512985No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA94550.054.055161
TACCTGG47750.041.4658282
GTATCAA167950.040.3234021
GTACCTG61700.032.708011
ACCTGGG59300.032.441513
ATCAACG216000.030.672763
CAACGCA216650.030.5936785
TATCAAC221150.030.381372
TCAACGC222400.029.811474
AACGCAG231400.029.008456
GTACATG212150.027.3208851
TACATGG213950.026.5133912
ACATGGG215650.025.5440943
ACGCAGA260850.025.4276857
CGCAGAG269300.024.5949868
GTGGTAT44500.024.468021
GTACTAG18050.024.4411431
TAACGCA6400.023.4609744
TGGTATC46050.023.230392
CCTGGGG60600.022.918974
Produced by FastQC (version 0.11.5)