##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211346_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5269249 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.12708452380975 33.0 31.0 34.0 30.0 34.0 2 32.254236419649175 34.0 31.0 34.0 30.0 34.0 3 32.324039535804815 34.0 31.0 34.0 30.0 34.0 4 35.95757744604592 37.0 35.0 37.0 35.0 37.0 5 35.87028644878995 37.0 35.0 37.0 35.0 37.0 6 35.8888243846514 37.0 35.0 37.0 35.0 37.0 7 35.805246819802974 37.0 35.0 37.0 35.0 37.0 8 35.788920394538195 37.0 35.0 37.0 33.0 37.0 9 37.40957278731751 39.0 37.0 39.0 34.0 39.0 10-11 37.35892505744177 39.0 37.0 39.0 34.0 39.0 12-13 37.29154553144101 39.0 37.0 39.0 34.0 39.0 14-15 38.53219633386086 40.0 38.0 41.0 34.0 41.0 16-17 38.52454875448095 40.0 38.0 41.0 34.0 41.0 18-19 38.539442432878005 40.0 38.0 41.0 34.0 41.0 20-21 38.50731375571737 40.0 38.0 41.0 34.0 41.0 22-23 38.393754214310235 40.0 38.0 41.0 34.0 41.0 24-25 38.30534370267945 40.0 38.0 41.0 34.0 41.0 26-27 38.20510693269573 40.0 38.0 41.0 33.5 41.0 28-29 38.09579600432623 40.0 37.5 41.0 33.0 41.0 30-31 38.07766286998394 40.0 37.0 41.0 33.0 41.0 32-33 37.99190634187149 40.0 37.0 41.0 33.0 41.0 34-35 37.848106153267764 40.0 37.0 41.0 33.0 41.0 36-37 37.68834116588531 40.0 37.0 41.0 33.0 41.0 38-39 37.56700698714371 40.0 37.0 41.0 32.5 41.0 40-41 37.45045508382694 40.0 36.0 41.0 32.0 41.0 42-43 37.33407787333641 40.0 36.0 41.0 32.0 41.0 44-45 37.16231221944531 40.0 36.0 41.0 31.0 41.0 46-47 36.90728479523363 39.0 36.0 41.0 31.0 41.0 48-49 35.32262301515833 39.0 35.0 41.0 24.0 41.0 50-51 36.07053063918596 39.0 35.0 40.0 28.0 41.0 52-53 36.17097284641511 39.0 35.0 40.0 29.0 41.0 54-55 36.00850510196045 38.5 35.0 40.0 28.5 41.0 56-57 35.800067428963786 38.0 35.0 40.0 28.0 41.0 58-59 35.57101799516401 38.0 34.0 40.0 28.0 41.0 60-61 35.31893406441791 37.5 34.0 40.0 28.0 41.0 62-63 35.00763922904383 37.0 34.0 40.0 27.0 41.0 64-65 34.67915057724545 36.5 34.0 39.5 26.0 41.0 66-67 34.34868175711567 36.0 33.0 39.0 26.0 40.5 68-69 33.99279100304426 35.5 33.0 39.0 26.0 40.0 70-71 33.6369590808861 35.0 33.0 38.0 26.0 40.0 72-73 33.25776168482453 35.0 32.0 37.5 25.0 40.0 74-75 32.89627705959616 35.0 32.0 37.0 25.0 39.0 76-77 31.956646004013095 34.0 30.5 36.0 23.5 38.5 78-79 32.379418679967486 35.0 31.5 36.0 25.0 38.5 80-81 32.24769649337126 35.0 32.0 36.0 25.0 37.5 82-83 31.974840627193743 34.5 31.0 35.0 24.0 37.0 84-85 31.68731749059496 34.0 31.0 35.0 24.0 36.5 86-87 31.42334068858769 34.0 31.0 35.0 23.0 36.0 88-89 31.189204286986627 34.0 31.0 35.0 22.5 36.0 90-91 30.968251168240485 34.0 30.5 35.0 20.5 35.0 92-93 30.78033672350652 34.0 30.0 35.0 20.0 35.0 94-95 30.58767539738585 34.0 30.0 35.0 20.0 35.0 96-97 30.396057199042975 34.0 29.5 35.0 19.5 35.0 98-99 30.176789235050386 34.0 29.0 35.0 19.0 35.0 100 27.8212504286664 31.0 25.0 34.0 11.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.31032310460767576 1101 2 -0.2865114904907884 1101 3 -0.30902958774283107 1101 4 -0.138154735074572 1101 5 -0.1669877807331872 1101 6 -0.22851158681380213 1101 7 -0.2416300292775233 1101 8 -0.17863699201123495 1101 9 -0.3216292911787022 1101 10-11 -0.3352195429781659 1101 12-13 -0.355458593852255 1101 14-15 -0.48778877920174324 1101 16-17 -0.46368858721080386 1101 18-19 -0.48003175011428567 1101 20-21 -0.46375231558946695 1101 22-23 -0.6017435816550858 1101 24-25 -0.4826521720487946 1101 26-27 -0.6252735740960134 1101 28-29 -0.6458321291743587 1101 30-31 -0.5223189120955354 1101 32-33 -0.5031277701274277 1101 34-35 -0.5747939519235601 1101 36-37 -0.5718450765921901 1101 38-39 -0.6172332526275 1101 40-41 -0.563099331621352 1101 42-43 -0.5676005353327938 1101 44-45 -0.5852630745729712 1101 46-47 -0.5859663730259612 1101 48-49 0.5497095014207432 1101 50-51 -0.4570292582685056 1101 52-53 -0.7420488687767559 1101 54-55 -0.7243892173465483 1101 56-57 -0.7071386709224399 1101 58-59 -0.8233884137570513 1101 60-61 -0.8110419705004901 1101 62-63 -0.7954350370299608 1101 64-65 -0.8555951666716695 1101 66-67 -0.8509728271960526 1101 68-69 -1.0160510400640135 1101 70-71 -0.9596797174136498 1101 72-73 -0.9480945686958506 1101 74-75 -0.9000346525243259 1101 76-77 -0.8816196722883625 1101 78-79 -0.8155416231243144 1101 80-81 -0.7105279075688742 1101 82-83 -0.6307162119807295 1101 84-85 -0.6228129492783694 1101 86-87 -0.6548719646253041 1101 88-89 -0.7471966955310592 1101 90-91 -0.8054591398611777 1101 92-93 -0.861784926721306 1101 94-95 -0.7882139932865542 1101 96-97 -0.9879671529981451 1101 98-99 -1.022006152420122 1101 100 -0.9265530179529975 1102 1 -0.31388550301836915 1102 2 -0.2944231426818327 1102 3 -0.2962219465849998 1102 4 -0.18307403827452617 1102 5 -0.21421552138696853 1102 6 -0.28249840277912597 1102 7 -0.270166979053414 1102 8 -0.2039596150266476 1102 9 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0.3524626323269757 2311 2 0.4035774374732384 2311 3 0.4203497901938107 2311 4 0.2891607069709181 2311 5 0.3276145656967273 2311 6 0.39173213634205695 2311 7 0.4291974187637564 2311 8 0.18574353896222107 2311 9 0.49643663370095226 2311 10-11 0.40053072366160336 2311 12-13 0.49036633192100254 2311 14-15 0.4918633640054324 2311 16-17 0.48693261137096044 2311 18-19 0.6445355007576978 2311 20-21 0.69439929433819 2311 22-23 0.7541970801880495 2311 24-25 0.6754489925183762 2311 26-27 0.7435447073026182 2311 28-29 0.801251048018564 2311 30-31 0.6843276244701428 2311 32-33 0.7155914221702844 2311 34-35 0.8509061576149364 2311 36-37 0.8645624210296745 2311 38-39 0.8835100957913937 2311 40-41 0.8354921618495794 2311 42-43 0.9191525130082638 2311 44-45 1.0103607904064233 2311 46-47 1.069065191356728 2311 48-49 -5.967862779574325 2311 50-51 -0.9490704216826487 2311 52-53 0.551470704023103 2311 54-55 0.9471956317497643 2311 56-57 1.0666776647265763 2311 58-59 1.086070811978729 2311 60-61 0.9330717765808814 2311 62-63 0.8536803675418341 2311 64-65 0.9271049572797878 2311 66-67 1.0239032213467993 2311 68-69 1.04260466314431 2311 70-71 1.1108493823528676 2311 72-73 0.6140234878606066 2311 74-75 0.831084009376454 2311 76-77 0.9728187987848322 2311 78-79 0.8823299429879299 2311 80-81 0.9248757273796713 2311 82-83 0.9266916892040165 2311 84-85 0.9684895499289183 2311 86-87 0.970245861323793 2311 88-89 0.9204519741527193 2311 90-91 1.0797877541459115 2311 92-93 1.073992003894702 2311 94-95 1.0924297615778187 2311 96-97 1.1411081114796744 2311 98-99 1.196175076140598 2311 100 1.3340527587614233 2312 1 0.07528719814335005 2312 2 0.27081856961429907 2312 3 0.2681963192191148 2312 4 -0.003003245429965773 2312 5 0.2109498857399359 2312 6 0.13038772539453447 2312 7 0.29460661185672876 2312 8 0.332309716618191 2312 9 0.3262399599258927 2312 10-11 0.27109571769359064 2312 12-13 0.2954596565233203 2312 14-15 0.3354222099142916 2312 16-17 0.46736739626279444 2312 18-19 0.3243036307105811 2312 20-21 0.4039738881172781 2312 22-23 0.6357445057533795 2312 24-25 0.7807858553687126 2312 26-27 0.6962574721148513 2312 28-29 0.7788006313083642 2312 30-31 0.822768071222562 2312 32-33 0.8527237911353964 2312 34-35 0.44336827403333245 2312 36-37 0.724733067154979 2312 38-39 0.8486183942347765 2312 40-41 0.6527516732449783 2312 42-43 0.7215347531873135 2312 44-45 0.8156418722198282 2312 46-47 0.8869456064758197 2312 48-49 -4.535502977839222 2312 50-51 -0.6639164481666242 2312 52-53 0.5517035297914461 2312 54-55 0.8012290247544129 2312 56-57 0.9108969034998751 2312 58-59 0.936221088639023 2312 60-61 1.007597775611508 2312 62-63 0.7552974268506318 2312 64-65 0.9585535680969741 2312 66-67 0.9567731392860495 2312 68-69 0.8394532905553405 2312 70-71 0.8456560536020916 2312 72-73 0.955063141889525 2312 74-75 0.6903842534004383 2312 76-77 0.8990663431851722 2312 78-79 0.5196641686778278 2312 80-81 0.721568341177452 2312 82-83 0.7468583522823486 2312 84-85 0.860597590012496 2312 86-87 0.8682689755991682 2312 88-89 0.8887073917004926 2312 90-91 0.7946499281176287 2312 92-93 0.9749923998363528 2312 94-95 1.0725295057911097 2312 96-97 1.1249626454579271 2312 98-99 1.2057540021725046 2312 100 0.659723640928874 2313 1 0.1081530826408823 2313 2 0.23672989471137384 2313 3 0.3406741561269442 2313 4 0.10696621735076661 2313 5 0.16700958713418146 2313 6 0.2770848369313086 2313 7 0.18427356604055944 2313 8 0.25377449476243896 2313 9 0.49973507561971786 2313 10-11 0.2263646118375533 2313 12-13 0.04106835726548752 2313 14-15 0.12194416312433276 2313 16-17 0.15005943410043443 2313 18-19 0.4719760806215092 2313 20-21 0.43396198999491986 2313 22-23 0.5604657752657687 2313 24-25 0.31624793470508195 2313 26-27 0.43948670272978063 2313 28-29 0.42589484425437263 2313 30-31 0.5846451191906894 2313 32-33 0.5148758441126375 2313 34-35 0.47663502850630834 2313 36-37 -0.32256391780426696 2313 38-39 0.17252202893584467 2313 40-41 0.3826743409803228 2313 42-43 0.5740365833856984 2313 44-45 0.5547521371484976 2313 46-47 0.480139200345441 2313 48-49 -5.282137255930234 2313 50-51 -1.2213675324988813 2313 52-53 0.18649750279168842 2313 54-55 0.3509349981324874 2313 56-57 0.39332031073595175 2313 58-59 0.5491103541958822 2313 60-61 0.7729274733202729 2313 62-63 0.8973464557168498 2313 64-65 0.9568009918832487 2313 66-67 1.0404783194902691 2313 68-69 1.031950996167346 2313 70-71 0.9593952658884106 2313 72-73 0.8115865852909678 2313 74-75 0.9189754982725873 2313 76-77 0.9165964012306027 2313 78-79 0.8346280569388327 2313 80-81 -0.07805141177902186 2313 82-83 0.31980581942128694 2313 84-85 0.6979283688495954 2313 86-87 0.8259236165267119 2313 88-89 0.8063020710097888 2313 90-91 0.8730848135060185 2313 92-93 0.9396412295319152 2313 94-95 0.8836388325139914 2313 96-97 0.8701230540281273 2313 98-99 1.0698053945085135 2313 100 1.1566466259183272 2314 1 0.006543277858959584 2314 2 0.15867759145986327 2314 3 0.18072842596007632 2314 4 0.05635445223065716 2314 5 0.21484025743514223 2314 6 0.2426681744354653 2314 7 0.10485384681732057 2314 8 0.1953330193983973 2314 9 0.29159409912708867 2314 10-11 0.30833692616759123 2314 12-13 0.21296632398139081 2314 14-15 0.24995048240798212 2314 16-17 0.46063662693259033 2314 18-19 0.45165586731689444 2314 20-21 0.583634751795465 2314 22-23 0.25735949951221926 2314 24-25 0.4157430296632114 2314 26-27 0.4257026303009468 2314 28-29 0.5217616171004806 2314 30-31 0.4110860388884774 2314 32-33 0.46819326332379063 2314 34-35 0.2572324065885283 2314 36-37 0.3718926126458584 2314 38-39 0.43875488646778393 2314 40-41 0.24245895965908915 2314 42-43 0.24567947738241003 2314 44-45 0.5027137457168678 2314 46-47 0.5266233855553821 2314 48-49 -6.305215087179846 2314 50-51 -1.6194832082137296 2314 52-53 -0.8024175104633358 2314 54-55 -0.4511770096118326 2314 56-57 -0.1669951565298433 2314 58-59 0.2865727310657533 2314 60-61 0.5619823473342862 2314 62-63 0.4453476436082937 2314 64-65 0.5551588337937972 2314 66-67 0.6731056040651922 2314 68-69 0.7330026987179323 2314 70-71 0.4466811503992858 2314 72-73 0.6340264443202059 2314 74-75 0.6565915175302237 2314 76-77 0.6480450776364286 2314 78-79 0.4110746699385217 2314 80-81 0.5312190569590953 2314 82-83 0.4454102049986126 2314 84-85 0.5000755599202904 2314 86-87 0.5486843909918093 2314 88-89 0.49498714139640043 2314 90-91 0.7408210942348745 2314 92-93 0.684066949623297 2314 94-95 0.740990185135054 2314 96-97 0.8082153045563345 2314 98-99 0.8544792279292039 2314 100 -0.4516129043108279 2315 1 0.14389786445822494 2315 2 0.24175761701953036 2315 3 0.31981721483445114 2315 4 0.12312001726151323 2315 5 0.1977823781540451 2315 6 0.26928698736168144 2315 7 0.339003551813434 2315 8 0.2541679354044035 2315 9 0.39390775439596126 2315 10-11 0.2661405002013808 2315 12-13 0.3967897784813559 2315 14-15 0.21795148572275025 2315 16-17 0.3348566332410101 2315 18-19 0.47048915935484814 2315 20-21 0.36493495319194835 2315 22-23 0.41020627272640553 2315 24-25 0.4770920397558598 2315 26-27 0.5350428509975913 2315 28-29 0.5737028192688101 2315 30-31 0.5484348183246794 2315 32-33 0.596436920475405 2315 34-35 0.6054835947137178 2315 36-37 0.551560991033277 2315 38-39 0.6091709258929185 2315 40-41 0.6804727917102227 2315 42-43 0.6628764636889102 2315 44-45 0.6430356907619483 2315 46-47 0.6114597213618183 2315 48-49 -4.973431332484193 2315 50-51 -0.9577371104235439 2315 52-53 0.2506644426669453 2315 54-55 0.23333418619102986 2315 56-57 0.5476102355331349 2315 58-59 0.6883736545880694 2315 60-61 0.541275837687877 2315 62-63 0.35364627319932396 2315 64-65 0.5160120344168106 2315 66-67 -0.2330945001784741 2315 68-69 0.5130757548668825 2315 70-71 0.4829548813202109 2315 72-73 0.588394113540943 2315 74-75 0.755812858467344 2315 76-77 0.7560689570984742 2315 78-79 0.7105200145498358 2315 80-81 0.49045389292813724 2315 82-83 0.45389095537374047 2315 84-85 0.5328183828780197 2315 86-87 0.5775531739652884 2315 88-89 0.5615021833750546 2315 90-91 0.6082909866413928 2315 92-93 0.716256054488845 2315 94-95 0.24162163770825984 2315 96-97 0.38869677746480136 2315 98-99 0.4112302893156041 2315 100 0.47302305788107546 2316 1 0.23372166945991069 2316 2 0.2836811015977929 2316 3 0.17765115471463133 2316 4 -0.014599123666371838 2316 5 0.14583741238656955 2316 6 0.1181378912803126 2316 7 0.2930626870908952 2316 8 0.2781097715057612 2316 9 0.44945499137948985 2316 10-11 0.4205836137296828 2316 12-13 0.34749817273355177 2316 14-15 0.4742736724575636 2316 16-17 0.46696597177629684 2316 18-19 0.13840646544943525 2316 20-21 0.48275356374939093 2316 22-23 0.6189691284924024 2316 24-25 0.6062726372614975 2316 26-27 0.6410881788190039 2316 28-29 0.6598532741241243 2316 30-31 0.601294638711316 2316 32-33 0.5774074559160738 2316 34-35 0.5391019050449302 2316 36-37 0.38998409001083445 2316 38-39 0.5006898612003283 2316 40-41 0.07364183235999633 2316 42-43 0.09582356162491124 2316 44-45 0.3669978985034348 2316 46-47 0.379091483056591 2316 48-49 -5.118957330026639 2316 50-51 -1.0421571970700612 2316 52-53 0.23201537001271078 2316 54-55 0.4683703018640628 2316 56-57 0.37818584178257453 2316 58-59 0.2711867019481744 2316 60-61 0.5551999808179104 2316 62-63 0.43382062742281136 2316 64-65 0.4232896196711593 2316 66-67 0.5656765745249785 2316 68-69 0.142141708936542 2316 70-71 -0.2823973078011903 2316 72-73 -0.18234145718697903 2316 74-75 0.16649152816883372 2316 76-77 0.14889557525068753 2316 78-79 0.2045711417340712 2316 80-81 0.1500731451299444 2316 82-83 0.028286109066716136 2316 84-85 0.2741337936873194 2316 86-87 0.3303200060640954 2316 88-89 0.12010399502047875 2316 90-91 0.19636110844854215 2316 92-93 0.2531978580912799 2316 94-95 0.4498482861861639 2316 96-97 0.5252584433714986 2316 98-99 0.48878081666544304 2316 100 -0.22659172522052273 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 2.0 10 9.0 11 12.0 12 29.0 13 123.0 14 489.0 15 1495.0 16 3751.0 17 7858.0 18 13322.0 19 19125.0 20 26144.0 21 35328.0 22 43149.0 23 45932.0 24 46636.0 25 49162.0 26 56384.0 27 68866.0 28 84568.0 29 103133.0 30 127419.0 31 156941.0 32 198074.0 33 256481.0 34 355410.0 35 503990.0 36 776274.0 37 1160551.0 38 985102.0 39 143466.0 40 24.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.22107950851182 19.45700902200686 13.649228549597309 23.672682919884007 2 15.64784765635379 20.521033792990888 40.22886087672751 23.602257673927813 3 18.693342215061172 26.114784945471314 30.008777348557526 25.18309549090999 4 12.501302673817499 16.737531489588765 38.06094937918695 32.70021645740679 5 12.985456285923263 37.63222550291144 34.74652215945516 14.63579605171014 6 30.63126477084866 37.1432009165636 18.062504946152647 14.16302936643509 7 27.3727778129926 32.33724521497003 22.345663876767187 17.94431309527018 8 26.056756362819243 35.39259843523915 20.22583586256948 18.324809339372123 9 26.507762705797255 15.822296016278726 20.715745408227466 36.95419586969655 10-11 25.07397234380406 26.311475425391983 28.029924472391343 20.584627758412616 12-13 25.403172201094904 24.04838569331632 28.43296196123789 22.11548014435089 14-15 22.75649462361102 25.422304494212927 26.360579688581453 25.4606211935946 16-17 21.847440532350976 28.173810198661837 27.26316489665806 22.71558437232913 18-19 21.598637680625835 27.201428514765574 29.582346554509005 21.617587250099586 20-21 22.548325234450097 26.474713170482676 29.469743767845298 21.50721782722193 22-23 22.438672555060407 26.36636756888167 29.179327817099843 22.01563205895808 24-25 22.024930696928802 26.478090702523527 29.575829335766574 21.921149264781096 26-27 21.967756104740232 26.642533546785778 29.327443559044646 22.06226678942934 28-29 21.77330111339461 26.795409778446484 29.504805377513595 21.92648373064531 30-31 22.290412183931082 26.584282640843682 29.41386239482083 21.71144278040441 32-33 22.144108391852278 26.828961142573966 29.12091525566239 21.906015209911374 34-35 22.265283034626233 26.798088932588747 29.30907927854738 21.62754875423764 36-37 22.322779100464444 26.66810614627739 29.064279724739862 21.944835028518305 38-39 22.10610885936227 26.860131991869977 29.22390355966138 21.809855589106377 40-41 22.143128950617342 26.500890594055175 29.387686932228497 21.968293523098982 42-43 22.00052591178366 26.637659591973307 29.084553841855804 22.27726065438723 44-45 21.931342454698278 27.014076829535167 29.01032677900116 22.044253936765397 46-47 21.981064578644975 26.79823440477403 28.664511188616544 22.556189827964456 48-49 22.30603906891951 26.93246641180239 28.495373063479377 22.26612145579871 50-51 22.132207704744957 27.022403491382207 28.363968850590116 22.481419953282717 52-53 21.946231531069905 27.29969315374382 28.223838416893127 22.53023689829315 54-55 22.011607482714375 27.163828884481568 28.234736222863972 22.58982740994009 56-57 22.178256438287526 27.150574980973154 28.035394686147125 22.635773894592198 58-59 21.937251669471063 27.120974861588753 28.390037635636666 22.551735833303518 60-61 22.1037690111457 27.335719436479327 28.11074588134348 22.44976567103149 62-63 22.060899952788894 27.187836141880044 28.127133099729306 22.62413080560175 64-65 21.843673471363395 27.049416898234774 28.1546608896676 22.95224874073423 66-67 21.908897147773875 27.01291021602769 28.009820173879536 23.068372462318905 68-69 21.754047391335217 27.12779205552921 28.020826491339506 23.097334061796065 70-71 21.849675167294986 26.89518173916931 28.083556803035307 23.171586290500393 72-73 21.966951002997877 27.117028156680917 27.852118912153003 23.063901928168203 74-75 21.954045426174897 27.28818894589064 27.701541533645624 23.05622409428884 76-77 21.846115767876604 27.21362125971541 27.67871573300899 23.261547239398997 78-79 21.7582973622332 27.174915868147153 27.743070403143932 23.323716366475715 80-81 21.640278351484938 27.491531512963284 27.550222822958066 23.317967312593712 82-83 21.920137132180194 27.215066318293836 27.760506869753183 23.104289679772787 84-85 22.005824043498947 27.302199444399044 27.371039748655534 23.320936763446472 86-87 21.575120170347905 27.49593421085726 27.65381068964421 23.275134929150628 88-89 21.725696208395124 27.164068485962684 27.639756151457647 23.470479154184538 90-91 21.86388459976468 27.16531804832779 27.587314720986107 23.383482630921424 92-93 21.79729121708272 27.244846450009714 27.482360587021592 23.47550174588597 94-95 21.409435543097118 27.085700013276025 27.67868945613185 23.826174987495012 96-97 21.521787299175806 27.115756250987484 27.62358804475365 23.738868405083064 98-99 21.351416297216947 27.259078194396384 27.486546728137327 23.90295878024934 100 21.40682850001348 27.11338851811731 27.439868747240098 24.039914234629116 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2978.0 1 2079.5 2 2942.5 3 4068.0 4 2562.5 5 1889.5 6 2718.0 7 4883.0 8 7275.5 9 8016.5 10 6772.0 11 5255.0 12 5016.0 13 5493.0 14 6045.5 15 6589.0 16 7170.0 17 7803.5 18 8424.5 19 8994.0 20 9927.5 21 11146.0 22 12644.0 23 14742.0 24 17454.5 25 20836.0 26 25257.0 27 30595.5 28 36338.5 29 44238.5 30 54693.0 31 65140.5 32 77646.0 33 92153.5 34 105830.5 35 118497.0 36 133511.0 37 150035.0 38 158608.0 39 160704.5 40 160731.0 41 159992.0 42 161721.5 43 167920.0 44 176093.5 45 186695.5 46 196015.5 47 200884.0 48 205978.0 49 208460.5 50 205627.0 51 200298.5 52 193747.0 53 186946.0 54 179997.0 55 167036.5 56 150095.0 57 131351.0 58 114056.5 59 100347.5 60 84205.0 61 67499.5 62 54049.5 63 42498.5 64 31597.0 65 22692.5 66 16625.0 67 12585.5 68 9497.5 69 7069.0 70 5474.0 71 4214.0 72 3272.5 73 2627.0 74 2024.0 75 1429.5 76 926.0 77 557.0 78 330.5 79 207.0 80 142.5 81 93.0 82 54.0 83 31.5 84 18.0 85 14.5 86 9.5 87 6.5 88 7.0 89 5.0 90 1.5 91 0.5 92 1.0 93 0.5 94 0.0 95 0.0 96 1.0 97 1.0 98 0.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.029871429495930062 2 0.0020686059816114213 3 7.59121461141806E-5 4 0.023494809222338896 5 5.883191323848996E-4 6 3.79560730570903E-5 7 5.6934109585635455E-5 8 0.003814585342237575 9 0.01009631543318602 10-11 9.489018264272575E-4 12-13 1.0437920090699831E-4 14-15 1.1386821917127091E-4 16-17 3.5109367577808526E-4 18-19 0.0 20-21 7.59121461141806E-5 22-23 2.562034931353595E-4 24-25 3.79560730570903E-5 26-27 6.642312784990803E-5 28-29 9.109457533701673E-4 30-31 0.0027518152966390466 32-33 0.009745221757407935 34-35 0.018769278126731154 36-37 0.021663428697334286 38-39 0.021283867966763386 40-41 0.005579542739392274 42-43 0.005778812122941998 44-45 0.009868578994843478 46-47 0.012478059017518436 48-49 1.2293877173008905 50-51 0.01827584917698898 52-53 0.018513074633595795 54-55 0.009346682990308485 56-57 0.007192675844318612 58-59 0.008340847054295593 60-61 0.005076624771385828 62-63 0.006860560205069071 64-65 0.018987525546809422 66-67 0.028875082578181447 68-69 0.029131286071316806 70-71 0.02625611353724221 72-73 0.022621819542025818 74-75 0.009536463355593938 76-77 0.01422403837814459 78-79 0.008274423926445685 80-81 0.002951084680188771 82-83 0.014337906597315861 84-85 0.023902837007702616 86-87 0.022897001071689724 88-89 0.03258528871951202 90-91 0.02048679043256449 92-93 0.01367367531881678 94-95 0.006689757876312165 96-97 0.0034445136299309444 98-99 0.015713814245635383 100 0.03520425776045125 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 5269249.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.08086292268654 #Duplication Level Percentage of deduplicated Percentage of total 1 76.82450295659126 48.4615594010826 2 14.56007394888625 18.369240578277456 3 4.274636262427666 8.089432323436343 4 1.6892609789649218 4.26240161018918 5 0.815812017603432 2.573106302656121 6 0.47164018097205607 1.7850881762837607 7 0.2998982104470615 1.324248653277906 8 0.19959070065915335 1.0072282903138414 9 0.13870567956597654 0.7874706563369505 >10 0.6676419369012425 7.2734698168574035 >50 0.03667105592536206 1.567194701041628 >100 0.020058444806475593 2.3149890632629933 >500 0.0010295985516983954 0.43343485136332915 >1k 3.309422521786415E-4 0.4663029972808558 >5k 3.677136135311991E-5 0.1274139364304704 >10k+ 1.1031408405935973E-4 1.1574186419092685 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20932 0.3972482606155071 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17248 0.32733317404434675 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11718 0.22238463204149206 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5493 0.1042463546512985 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.897803652854515E-5 2 0.0 0.0 0.0 0.0 1.897803652854515E-5 3 0.0 0.0 0.0 0.0 1.897803652854515E-5 4 0.0 0.0 0.0 0.0 1.897803652854515E-5 5 0.0 0.0 0.0 0.0 1.897803652854515E-5 6 0.0 0.0 0.0 0.0 1.897803652854515E-5 7 0.0 0.0 0.0 0.0 1.897803652854515E-5 8 0.0 0.0 0.0 0.0 1.897803652854515E-5 9 0.0 0.0 0.0 0.0 1.897803652854515E-5 10-11 0.0 0.0 0.0 9.489018264272575E-6 1.897803652854515E-5 12-13 0.0 0.0 0.0 1.897803652854515E-5 3.79560730570903E-5 14-15 0.0 4.744509132136287E-5 0.0 4.744509132136287E-5 6.642312784990803E-5 16-17 0.0 7.59121461141806E-5 0.0 6.642312784990803E-5 9.489018264272575E-5 18-19 0.0 7.59121461141806E-5 0.0 1.1386821917127091E-4 1.3284625569981605E-4 20-21 0.0 7.59121461141806E-5 0.0 2.0875840181399665E-4 1.3284625569981605E-4 22-23 0.0 7.59121461141806E-5 0.0 4.6496189494935617E-4 1.423352739640886E-4 24-25 0.0 7.59121461141806E-5 0.0 0.001091237100391346 1.518242922283612E-4 26-27 0.0 7.59121461141806E-5 0.0 0.0024671447487108695 1.518242922283612E-4 28-29 0.0 7.59121461141806E-5 0.0 0.006547422602348077 1.518242922283612E-4 30-31 0.0 7.59121461141806E-5 0.0 0.021511604405105925 1.518242922283612E-4 32-33 0.0 7.59121461141806E-5 0.0 0.0550837510241023 1.518242922283612E-4 34-35 0.0 7.59121461141806E-5 0.0 0.10613466928588874 1.897803652854515E-4 36-37 0.0 7.59121461141806E-5 0.0 0.18228404085667615 1.897803652854515E-4 38-39 0.0 8.540116437845318E-5 0.0 0.29504204489102714 1.897803652854515E-4 40-41 0.0 9.489018264272575E-5 0.0 0.4164825006371876 1.897803652854515E-4 42-43 0.0 1.138682191712709E-4 0.0 0.5348485144657236 1.897803652854515E-4 44-45 0.0 1.138682191712709E-4 0.0 0.6808180824250287 2.0875840181399665E-4 46-47 0.0 1.138682191712709E-4 0.0 0.8503583717527867 2.3722545660681435E-4 48-49 0.0 1.2335723743554347E-4 0.0 1.0335628473810974 2.4671447487108693E-4 50-51 0.0 1.3284625569981605E-4 0.0 1.2263607204745877 2.4671447487108693E-4 52-53 0.0 1.3284625569981605E-4 0.0 1.4128199293675436 2.562034931353595E-4 54-55 0.0 1.3284625569981605E-4 0.0 1.6223754087157392 2.656925113996321E-4 56-57 0.0 1.3284625569981605E-4 0.0 1.8687862350023694 2.7518152966390463E-4 58-59 0.0 1.3284625569981605E-4 0.0 2.1368130448950122 2.846705479281772E-4 60-61 0.0 1.3284625569981605E-4 0.0 2.4048303657693912 2.846705479281772E-4 62-63 0.0 1.3284625569981605E-4 0.0 2.677497305593264 3.2262662098526756E-4 64-65 0.0 1.3284625569981605E-4 0.0 2.95579123324785 3.2262662098526756E-4 66-67 0.0 1.7080232875690634E-4 0.0 3.2679894231606816 3.3211563924954014E-4 68-69 0.0 1.7080232875690634E-4 0.0 3.6127349457199687 3.416046575138127E-4 70-71 0.0 1.8978036528545148E-4 0.0 3.9777774783465345 3.5109367577808526E-4 72-73 0.0 2.0875840181399665E-4 0.0 4.340295932114804 3.79560730570903E-4 74-75 0.0 2.0875840181399665E-4 0.0 4.697842140312595 3.79560730570903E-4 76-77 0.0 2.277364383425418E-4 0.0 5.0830298587142115 3.79560730570903E-4 78-79 0.0 2.277364383425418E-4 0.0 5.508223278118002 3.79560730570903E-4 80-81 0.0 2.277364383425418E-4 0.0 5.950857513091524 3.9853876709944813E-4 82-83 0.0 2.277364383425418E-4 0.0 6.4048406139091165 4.364948401565384E-4 84-85 0.0 2.277364383425418E-4 0.0 6.841667569704905 4.364948401565384E-4 86-87 0.0 2.277364383425418E-4 0.0 7.299104673170692 4.364948401565384E-4 88 0.0 2.277364383425418E-4 0.0 7.659706345249579 4.364948401565384E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9455 0.0 54.05516 1 TACCTGG 4775 0.0 41.465828 2 GTATCAA 16795 0.0 40.323402 1 GTACCTG 6170 0.0 32.70801 1 ACCTGGG 5930 0.0 32.44151 3 ATCAACG 21600 0.0 30.67276 3 CAACGCA 21665 0.0 30.593678 5 TATCAAC 22115 0.0 30.38137 2 TCAACGC 22240 0.0 29.81147 4 AACGCAG 23140 0.0 29.00845 6 GTACATG 21215 0.0 27.320885 1 TACATGG 21395 0.0 26.513391 2 ACATGGG 21565 0.0 25.544094 3 ACGCAGA 26085 0.0 25.427685 7 CGCAGAG 26930 0.0 24.594986 8 GTGGTAT 4450 0.0 24.46802 1 GTACTAG 1805 0.0 24.441143 1 TAACGCA 640 0.0 23.460974 4 TGGTATC 4605 0.0 23.23039 2 CCTGGGG 6060 0.0 22.91897 4 >>END_MODULE