FastQCFastQC Report
Mon 6 Jun 2016
ERR1211345_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211345_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10777017
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT681060.6319559484781364No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT488570.4533443716382743No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT311510.2890503002825365No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC137560.12764199963681971No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108570.10074216269678335No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA275700.051.8474661
GTATCAA544950.044.1578061
ATCAACG665400.035.0713163
CAACGCA666250.035.0055735
GTACTAG83350.034.4237251
TATCAAC704150.033.812752
GCATCGT80350.032.924448
TATTCTC64350.032.56995
AACGCAG728950.032.510036
TAGGCAT98350.032.347785
TCAACGC759350.030.9485954
CTGTGCG105050.029.6586699
TACCTGG32500.029.5236682
ACGCAGA792350.029.398697
TACCCCG5300.029.2595885
CGCAGAG795050.029.219988
CTAGGCA110350.028.9150224
ACCTGGG32850.028.6103233
ACTGTGC113750.028.2140338
GGGCACC102400.027.82807794
Produced by FastQC (version 0.11.5)