FastQCFastQC Report
Mon 6 Jun 2016
ERR1211345_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211345_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10777017
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT592620.5498924238497536No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT449130.4167479739523469No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT295960.27462144673243066No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC137110.127224444389389No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA279850.051.799911
GTATCAA534950.042.564151
TACCCCG5700.035.3890385
TACCTGG35800.034.2057532
ACCTGGG35750.033.468643
ATCAACG663500.033.3649833
CAACGCA666250.033.1704255
TATCAAC705850.032.1185652
TAGGCAT100600.031.5693655
AACGCAG727700.030.8396856
CTGTGCG108100.029.4220629
TCAACGC756350.029.3996284
TATTCTC65500.028.361495
CTAGGCA113200.028.0621014
TAGTACT114200.027.7752844
ACTGTGC116900.027.7291568
ACGCAGA798900.027.621557
GGGCACC106900.027.5688394
CGCAGAG803350.027.421838
CATGGGT45300.026.9310384
Produced by FastQC (version 0.11.5)