##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211345_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 10777017 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.12594032281846 33.0 31.0 34.0 30.0 34.0 2 32.26109330624606 33.0 31.0 34.0 30.0 34.0 3 32.3190537789817 34.0 31.0 34.0 30.0 34.0 4 35.95821339058851 37.0 35.0 37.0 35.0 37.0 5 35.87988939796606 37.0 35.0 37.0 35.0 37.0 6 35.90255856513913 37.0 35.0 37.0 35.0 37.0 7 35.82140957929268 37.0 35.0 37.0 35.0 37.0 8 35.81143659697298 37.0 35.0 37.0 35.0 37.0 9 37.4081467070155 39.0 37.0 39.0 34.0 39.0 10-11 37.36369901801213 39.0 37.0 39.0 34.0 39.0 12-13 37.28868025354326 39.0 37.0 39.0 34.0 39.0 14-15 38.47399475198007 40.0 38.0 41.0 34.0 41.0 16-17 38.48582019495747 40.0 38.0 41.0 34.0 41.0 18-19 38.54591149851578 40.0 38.0 41.0 34.0 41.0 20-21 38.53464163599259 40.0 38.0 41.0 34.0 41.0 22-23 38.41580921696607 40.0 38.0 41.0 34.0 41.0 24-25 38.32026144154732 40.0 38.0 41.0 34.0 41.0 26-27 38.19596006947006 40.0 38.0 41.0 33.5 41.0 28-29 38.11479702593027 40.0 37.0 41.0 33.0 41.0 30-31 38.07858895462446 40.0 37.0 41.0 33.0 41.0 32-33 37.969970261715275 40.0 37.0 41.0 33.0 41.0 34-35 37.83059458846543 40.0 37.0 41.0 33.0 41.0 36-37 37.683127251260714 40.0 36.0 41.0 33.0 41.0 38-39 37.55022702478803 40.0 36.0 41.0 33.0 41.0 40-41 37.41441755172141 40.0 36.0 41.0 32.0 41.0 42-43 37.276947600620844 40.0 36.0 41.0 32.0 41.0 44-45 37.04096504626466 39.0 35.5 41.0 31.0 41.0 46-47 36.70629553613955 39.0 35.0 41.0 30.5 41.0 48-49 35.068030884613066 39.0 35.0 40.5 22.5 41.0 50-51 35.749346178075065 38.5 34.5 40.0 27.5 41.0 52-53 35.82679938242651 38.0 35.0 40.0 28.0 41.0 54-55 35.6509666357583 38.0 35.0 40.0 28.0 41.0 56-57 35.408313543534355 38.0 34.0 40.0 27.5 41.0 58-59 35.149524771093894 37.5 34.0 40.0 27.0 41.0 60-61 34.90789705537256 37.0 34.0 40.0 26.5 41.0 62-63 34.58898626586559 36.5 34.0 40.0 26.0 41.0 64-65 34.26972649296183 36.0 33.0 39.0 26.0 41.0 66-67 33.96209883495591 35.5 33.0 39.0 25.5 40.5 68-69 33.64303582336373 35.0 33.0 39.0 25.0 40.0 70-71 33.36369150201767 35.0 32.5 38.0 25.0 40.0 72-73 33.038146826714666 35.0 32.0 37.5 24.0 40.0 74-75 32.69518986561866 35.0 32.0 37.0 24.0 39.0 76-77 31.756436498151572 34.0 30.5 36.0 22.5 39.0 78-79 32.20333019795737 35.0 31.5 36.0 23.5 39.0 80-81 32.09493461873541 35.0 32.0 36.0 23.5 37.5 82-83 31.836775519608068 35.0 31.0 35.0 23.0 37.0 84-85 31.553851125965565 34.0 31.0 35.0 22.5 37.0 86-87 31.312213760078507 34.0 31.0 35.0 21.0 36.0 88-89 31.09368756679144 34.0 31.0 35.0 20.0 36.0 90-91 30.87758356509969 34.0 31.0 35.0 20.0 36.0 92-93 30.678742410817392 34.0 30.0 35.0 20.0 35.0 94-95 30.49852955599866 34.0 30.0 35.0 19.0 35.0 96-97 30.328225565571625 34.0 29.5 35.0 19.0 35.0 98-99 30.13241103730281 34.0 29.0 35.0 18.0 35.0 100 27.657836950614442 31.0 25.0 34.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.2659196911380093 1101 2 -0.2382048888760515 1101 3 -0.24976582984145068 1101 4 -0.15662760284687494 1101 5 -0.12920723298085335 1101 6 -0.20333066430231383 1101 7 -0.16764770636599735 1101 8 -0.2098127062910038 1101 9 -0.3126899331459967 1101 10-11 -0.34298050300412797 1101 12-13 -0.3371387479944161 1101 14-15 -0.4357833153948718 1101 16-17 -0.446068086690353 1101 18-19 -0.48220525510627255 1101 20-21 -0.5409474395958398 1101 22-23 -0.5882338135108753 1101 24-25 -0.47596180561919965 1101 26-27 -0.5850188713363664 1101 28-29 -0.557587260485839 1101 30-31 -0.4742469703735068 1101 32-33 -0.4665342972174997 1101 34-35 -0.4938814815878274 1101 36-37 -0.4946408468974468 1101 38-39 -0.4255757059452492 1101 40-41 -0.3720478278786601 1101 42-43 -0.479232328777357 1101 44-45 -0.4506100318871802 1101 46-47 -0.46774796707848765 1101 48-49 0.7361774640936858 1101 50-51 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1105 68-69 -0.5901534622816698 1105 70-71 -0.6170941708154558 1105 72-73 -0.5128746934856352 1105 74-75 -0.6327829836613681 1105 76-77 -0.617593394736538 1105 78-79 -0.5348818028857281 1105 80-81 -0.49691608104609486 1105 82-83 -0.47163535474755136 1105 84-85 -0.5297568513347208 1105 86-87 -0.4365565466922767 1105 88-89 -0.4875696798598952 1105 90-91 -0.5456504132985067 1105 92-93 -0.5689319007204752 1105 94-95 -0.5316007799322975 1105 96-97 -0.4945521592324802 1105 98-99 -0.5841525412119779 1105 100 -0.6530127485510704 1106 1 -0.31875592023570576 1106 2 -0.2928267244474654 1106 3 -0.3377888763047139 1106 4 -0.21038154254161867 1106 5 -0.27467350434096005 1106 6 -0.344045127653132 1106 7 -0.3245328523504796 1106 8 -0.37678651769706306 1106 9 -0.45090672317572 1106 10-11 -0.47025803780928044 1106 12-13 -0.4562092571562104 1106 14-15 -0.611294927924348 1106 16-17 -0.5815924171123683 1106 18-19 -0.6246394046194723 1106 20-21 -0.6615177925408346 1106 22-23 -0.6712549797313798 1106 24-25 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0.8647505888703151 2205 38-39 0.8768013963059005 2205 40-41 0.930678400283071 2205 42-43 0.9411227923081 2205 44-45 0.9024541284771459 2205 46-47 0.9659217388372454 2205 48-49 2.209120541796274 2205 50-51 1.3050850248225885 2205 52-53 1.0684712742278677 2205 54-55 1.0038039508690346 2205 56-57 0.9874863463466994 2205 58-59 0.9549121057097025 2205 60-61 0.9265216518823678 2205 62-63 1.0265627821445733 2205 64-65 1.022527501344591 2205 66-67 0.9940232967908074 2205 68-69 0.9949178062000996 2205 70-71 1.0032796609477685 2205 72-73 1.0517090461783098 2205 74-75 0.964803718499617 2205 76-77 1.0051962866490989 2205 78-79 0.9019677536359296 2205 80-81 0.782408756450657 2205 82-83 0.8099463215516813 2205 84-85 0.8871545524726798 2205 86-87 0.9714481274940034 2205 88-89 0.9504828823221558 2205 90-91 1.0289506784091706 2205 92-93 1.032940679387977 2205 94-95 1.0245038415852719 2205 96-97 1.0488972187461094 2205 98-99 1.0643749967325107 2205 100 1.2989929119020012 2206 1 0.5763909951661361 2206 2 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0.9842963709120625 2206 64-65 1.0284308115727114 2206 66-67 1.0398358460879535 2206 68-69 1.0781052606596262 2206 70-71 0.9852305139177702 2206 72-73 1.0487692364756782 2206 74-75 1.045357619521873 2206 76-77 1.021222591388554 2206 78-79 0.9747804115827634 2206 80-81 0.8960494852235925 2206 82-83 0.9196085346304734 2206 84-85 0.9506998907927766 2206 86-87 0.9289723464721469 2206 88-89 0.9259009095671544 2206 90-91 0.9587513249444264 2206 92-93 0.9251587054465134 2206 94-95 0.9512812900793861 2206 96-97 1.0383920999718406 2206 98-99 1.0998418270026207 2206 100 1.3971432669107138 2207 1 0.5282644358321065 2207 2 0.5222172157895457 2207 3 0.5003869595669599 2207 4 0.3230459172848157 2207 5 0.3986402620412903 2207 6 0.40107585955437486 2207 7 0.4468125988969973 2207 8 0.4272939790173922 2207 9 0.5661734400191918 2207 10-11 0.6022025455726876 2207 12-13 0.6075339840013996 2207 14-15 0.7298843720572421 2207 16-17 0.7015512244841418 2207 18-19 0.681016918299143 2207 20-21 0.6948600700655376 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0.9714963980644065 2207 90-91 1.0319940640596066 2207 92-93 0.9651789498108947 2207 94-95 0.9814403929544042 2207 96-97 1.0327885322470358 2207 98-99 1.0573783926792686 2207 100 1.2702511797049532 2208 1 0.5735485508915943 2208 2 0.54710508281741 2208 3 0.4990685663935821 2208 4 0.3226761456034666 2208 5 0.3801965051612797 2208 6 0.4159768972755913 2208 7 0.45753736259414524 2208 8 0.4724853507471778 2208 9 0.5749687609216707 2208 10-11 0.6173679876632931 2208 12-13 0.6291903322117136 2208 14-15 0.7969971752579497 2208 16-17 0.7665005473251156 2208 18-19 0.7421247792939667 2208 20-21 0.7303013961687483 2208 22-23 0.7647641896144322 2208 24-25 0.7555096328694901 2208 26-27 0.8016719451381746 2208 28-29 0.8055438098444725 2208 30-31 0.8344115658781206 2208 32-33 0.8707423086975936 2208 34-35 0.8735124464170383 2208 36-37 0.8780932841518947 2208 38-39 0.8593798638208412 2208 40-41 0.9158419344211808 2208 42-43 0.9139864161198474 2208 44-45 0.9170767224206031 2208 46-47 0.9194144730677252 2208 48-49 2.1462994020747175 2208 50-51 1.3202137337093518 2208 52-53 1.0098536297444198 2208 54-55 0.9598433103813662 2208 56-57 0.9846909542544751 2208 58-59 0.9905797438932993 2208 60-61 0.9892624540150337 2208 62-63 0.9298643576679311 2208 64-65 0.9415633877573342 2208 66-67 0.9137567284319843 2208 68-69 0.9016674289965536 2208 70-71 0.9421975019960556 2208 72-73 1.0041631996574267 2208 74-75 1.027220654535391 2208 76-77 0.9246827687809223 2208 78-79 0.8096306413946479 2208 80-81 0.8027553569832904 2208 82-83 0.7902617542868917 2208 84-85 0.8435784305034133 2208 86-87 0.9223092335436824 2208 88-89 0.8845901071915456 2208 90-91 0.8830305676807519 2208 92-93 0.8983688811779089 2208 94-95 0.9346834369687613 2208 96-97 0.9895046309975477 2208 98-99 1.0942599491688298 2208 100 1.3414806299137894 2209 1 0.310822395428616 2209 2 0.365449594324339 2209 3 0.39333219633179795 2209 4 0.2372529058630235 2209 5 0.3387321173208022 2209 6 0.38010547785410864 2209 7 0.40753185634916633 2209 8 0.44036687457683144 2209 9 0.5986771142703162 2209 10-11 0.576868555040214 2209 12-13 0.6156971134056519 2209 14-15 0.7135844528036657 2209 16-17 0.7312725365570003 2209 18-19 0.7226297896153611 2209 20-21 0.7248829888295774 2209 22-23 0.7304004683493943 2209 24-25 0.7604447639265643 2209 26-27 0.817517855260256 2209 28-29 0.8291404015061161 2209 30-31 0.7935863073792575 2209 32-33 0.7739315443262029 2209 34-35 0.8065808916682116 2209 36-37 0.8489888172978439 2209 38-39 0.8586702441574587 2209 40-41 0.8407991316079304 2209 42-43 0.8893255445089778 2209 44-45 0.8576621271396618 2209 46-47 0.8339150241096362 2209 48-49 2.0352384967341948 2209 50-51 1.2849679352030279 2209 52-53 1.0265067422991834 2209 54-55 0.9857593021542286 2209 56-57 0.9681041808918778 2209 58-59 0.8772963014187738 2209 60-61 0.8376636877005055 2209 62-63 0.8994543852071786 2209 64-65 0.893978022932508 2209 66-67 0.9399305635275752 2209 68-69 0.9408541745258745 2209 70-71 0.9443483853087713 2209 72-73 0.9581924237543831 2209 74-75 1.0299851918093097 2209 76-77 0.9169019742965254 2209 78-79 0.8761296405517172 2209 80-81 0.8443755945965776 2209 82-83 0.8413436191836077 2209 84-85 0.9203992380447659 2209 86-87 0.9021723273833935 2209 88-89 0.8893042620839751 2209 90-91 0.9331132625864562 2209 92-93 0.8866600663472326 2209 94-95 0.8327287722602996 2209 96-97 0.9602308848255312 2209 98-99 0.9566634326942349 2209 100 1.237824110533868 2210 1 0.5441406811776943 2210 2 0.4660754694849345 2210 3 0.46231895649746946 2210 4 0.3108174306574867 2210 5 0.3491875993801301 2210 6 0.41768876129034993 2210 7 0.4374321829711789 2210 8 0.4722322132902619 2210 9 0.6186210671185037 2210 10-11 0.5979502166638113 2210 12-13 0.6055773972416816 2210 14-15 0.7730764952362819 2210 16-17 0.7531774316663942 2210 18-19 0.7283310431309147 2210 20-21 0.7545966818645056 2210 22-23 0.7571961617747078 2210 24-25 0.762368352011265 2210 26-27 0.7826050276889376 2210 28-29 0.8066139305459146 2210 30-31 0.8020225358270068 2210 32-33 0.783279944308255 2210 34-35 0.7969062482127995 2210 36-37 0.8195964545195764 2210 38-39 0.8638074878542668 2210 40-41 0.869739527483361 2210 42-43 0.8545922013531353 2210 44-45 0.8699794535464065 2210 46-47 0.9075092929884718 2210 48-49 2.154643215713108 2210 50-51 1.3038940578406795 2210 52-53 1.009664186397174 2210 54-55 1.02094901547499 2210 56-57 1.010499072574376 2210 58-59 0.934519565517526 2210 60-61 0.9073860378036684 2210 62-63 0.9586734253909768 2210 64-65 0.9898676552567807 2210 66-67 0.9590863128501965 2210 68-69 0.9321484056366884 2210 70-71 0.9744067390246016 2210 72-73 0.9712908535491565 2210 74-75 0.9908791740465617 2210 76-77 1.0134601767519662 2210 78-79 0.8806996970201695 2210 80-81 0.9015888949664443 2210 82-83 0.9037065492636494 2210 84-85 0.9087812163423621 2210 86-87 0.9201500619013281 2210 88-89 0.9127206725180521 2210 90-91 0.9998624889460608 2210 92-93 0.9809178928955049 2210 94-95 1.0419097482538326 2210 96-97 1.112536905538164 2210 98-99 1.1646936764556877 2210 100 1.3757503423802682 2211 1 0.5380214400455543 2211 2 0.5032808437418481 2211 3 0.4739569281848972 2211 4 0.3007147778159762 2211 5 0.3420857747793562 2211 6 0.3861501022990126 2211 7 0.4310349910820932 2211 8 0.4613220669086999 2211 9 0.6197072064210971 2211 10-11 0.6148314650215454 2211 12-13 0.547394437378621 2211 14-15 0.7184171493982063 2211 16-17 0.666504840977943 2211 18-19 0.6997456688492889 2211 20-21 0.7076738037011481 2211 22-23 0.721803323503643 2211 24-25 0.7415944287860086 2211 26-27 0.8062254364304806 2211 28-29 0.7890611909471019 2211 30-31 0.7706077036662222 2211 32-33 0.7601295266665247 2211 34-35 0.771015101739799 2211 36-37 0.8068318658887463 2211 38-39 0.8491131647011727 2211 40-41 0.8121959470286839 2211 42-43 0.7446637580997688 2211 44-45 0.721670252866673 2211 46-47 0.858328212935362 2211 48-49 2.094203546935262 2211 50-51 1.224543183801643 2211 52-53 0.9833792420305727 2211 54-55 0.955979145769497 2211 56-57 0.8962542970601532 2211 58-59 0.9051001330374788 2211 60-61 0.8715601724341795 2211 62-63 0.854438429377737 2211 64-65 0.9214795678380554 2211 66-67 0.926357920110874 2211 68-69 0.9527007765221427 2211 70-71 0.9202972327071564 2211 72-73 0.9704014394419715 2211 74-75 0.9722809143611713 2211 76-77 0.7242635857183792 2211 78-79 0.553855244832846 2211 80-81 0.6862284887454564 2211 82-83 0.785274313544928 2211 84-85 0.8171282026745317 2211 86-87 0.8248277404426041 2211 88-89 0.9011673113268408 2211 90-91 0.9205605355787725 2211 92-93 0.9343361852574859 2211 94-95 0.935434865035365 2211 96-97 0.9532312876895155 2211 98-99 1.0008140594562818 2211 100 1.2802441404592102 2212 1 0.4955479221924932 2212 2 0.4711883717635388 2212 3 0.4844387738571143 2212 4 0.2672292964080185 2212 5 0.32149607659412993 2212 6 0.2743329649549011 2212 7 0.21014293878513257 2212 8 0.3336920147936695 2212 9 0.5172053841257807 2212 10-11 0.494041397366189 2212 12-13 0.5328479222593003 2212 14-15 0.6454649877192509 2212 16-17 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2310 100 1.1797019713262422 2311 1 0.3550117690355492 2311 2 0.35381778157022836 2311 3 0.39415137621868723 2311 4 0.2658300368400859 2311 5 0.30536622943572667 2311 6 0.33196343842097775 2311 7 0.36586040519852503 2311 8 0.1573590305908894 2311 9 0.40671643554683357 2311 10-11 0.3762866775340754 2311 12-13 0.4630078903745485 2311 14-15 0.4833219236544153 2311 16-17 0.47538320791017696 2311 18-19 0.5721886822598847 2311 20-21 0.604651253702464 2311 22-23 0.5927877160165096 2311 24-25 0.6031666390045487 2311 26-27 0.6625053690610301 2311 28-29 0.697780167296294 2311 30-31 0.5600223639245101 2311 32-33 0.5720708855912306 2311 34-35 0.6731730287128457 2311 36-37 0.6892003729359928 2311 38-39 0.7241296721747048 2311 40-41 0.6936745643479583 2311 42-43 0.7131434218928234 2311 44-45 0.7131629276028946 2311 46-47 0.7811084237444916 2311 48-49 -5.982370831633386 2311 50-51 -1.1120938820952517 2311 52-53 0.28003435135953936 2311 54-55 0.6837242675346218 2311 56-57 0.7476039449375804 2311 58-59 0.7316083632372781 2311 60-61 0.6305784527103313 2311 62-63 0.5450654306127163 2311 64-65 0.6370233893092276 2311 66-67 0.7202432886340304 2311 68-69 0.7947508588804837 2311 70-71 0.7743206437272363 2311 72-73 0.321069805193666 2311 74-75 0.6341680224087227 2311 76-77 0.6594206763127666 2311 78-79 0.5758042214168739 2311 80-81 0.6580120299044623 2311 82-83 0.6991986465508511 2311 84-85 0.7108427017335259 2311 86-87 0.7121324033220837 2311 88-89 0.7484199859324683 2311 90-91 0.8329885069161129 2311 92-93 0.7966827197253536 2311 94-95 0.8008732466647714 2311 96-97 0.8570400673275351 2311 98-99 0.8725081811844433 2311 100 1.0447484965892855 2312 1 0.06574036594624744 2312 2 0.20368665283393028 2312 3 0.20712366943720184 2312 4 -0.02080176790706645 2312 5 0.18067368409969475 2312 6 0.11946297868139766 2312 7 0.25425991778894996 2312 8 0.3081590141503554 2312 9 0.2647747467535453 2312 10-11 0.19507378952712173 2312 12-13 0.2764751426862304 2312 14-15 0.3496005211889752 2312 16-17 0.465196561389682 2312 18-19 0.29803290479520683 2312 20-21 0.32392468657766926 2312 22-23 0.6038039733049558 2312 24-25 0.6476799906981157 2312 26-27 0.569362069845873 2312 28-29 0.6705216981593551 2312 30-31 0.692430582983512 2312 32-33 0.7112619555607793 2312 34-35 0.33343555367027733 2312 36-37 0.6308920612603757 2312 38-39 0.708648212095639 2312 40-41 0.5229856555010031 2312 42-43 0.5465563788586678 2312 44-45 0.6618893450014127 2312 46-47 0.7324299130446477 2312 48-49 -4.673207832485669 2312 50-51 -0.8331334896688816 2312 52-53 0.4304785413400012 2312 54-55 0.7200029902488225 2312 56-57 0.7799111395377736 2312 58-59 0.7683176611164768 2312 60-61 0.7400623133843212 2312 62-63 0.494845518111255 2312 64-65 0.7515454350757196 2312 66-67 0.8275839168009895 2312 68-69 0.6848987274381102 2312 70-71 0.6696461788987591 2312 72-73 0.7728665184247134 2312 74-75 0.42825998353502115 2312 76-77 0.6764131505288198 2312 78-79 0.410518394288907 2312 80-81 0.6036797112194279 2312 82-83 0.5760333948219234 2312 84-85 0.6187260285142955 2312 86-87 0.6962299604270363 2312 88-89 0.8203144685437884 2312 90-91 0.6019000217879835 2312 92-93 0.7936596924607358 2312 94-95 0.874777382461609 2312 96-97 0.9415567662514093 2312 98-99 0.9666215309044901 2312 100 0.6831075194769376 2313 1 0.03177278650247217 2313 2 0.17573392702902169 2313 3 0.22891946743939684 2313 4 0.01457366180738262 2313 5 0.11261950979338309 2313 6 0.24170118689993814 2313 7 0.15732117657142197 2313 8 0.2432249840845344 2313 9 0.43223862374920685 2313 10-11 0.22980731576362956 2313 12-13 -0.02330903257944783 2313 14-15 0.048888229792183324 2313 16-17 0.04958607451658992 2313 18-19 0.392463818773237 2313 20-21 0.3521655285473031 2313 22-23 0.46426875910267285 2313 24-25 0.2323173279012707 2313 26-27 0.32748023154626793 2313 28-29 0.30595131690936483 2313 30-31 0.43186741977054766 2313 32-33 0.34967959187623165 2313 34-35 0.24270426207757367 2313 36-37 -0.4137480250073011 2313 38-39 0.08640619685399997 2313 40-41 0.2733923046427549 2313 42-43 0.3287884283640423 2313 44-45 0.3456760107690968 2313 46-47 0.21475375709722044 2313 48-49 -5.447121428210586 2313 50-51 -1.4112403086721343 2313 52-53 -0.04321239210583627 2313 54-55 0.11329633684319873 2313 56-57 0.12447297244558797 2313 58-59 0.22995769594994186 2313 60-61 0.5430351100775823 2313 62-63 0.5907656870159954 2313 64-65 0.6686161155052233 2313 66-67 0.6902329361564696 2313 68-69 0.708598791831939 2313 70-71 0.6793283477372469 2313 72-73 0.48857649725189134 2313 74-75 0.579271245707055 2313 76-77 0.6220413647393492 2313 78-79 0.5155624550676947 2313 80-81 -0.31003676525099166 2313 82-83 0.07514775981756117 2313 84-85 0.4422723587514419 2313 86-87 0.5905442620055474 2313 88-89 0.5860538244616471 2313 90-91 0.5898646006936836 2313 92-93 0.6351298090884967 2313 94-95 0.5529768414337397 2313 96-97 0.5693437565875037 2313 98-99 0.7535903671647404 2313 100 0.8108199774900662 2314 1 0.001451215204220091 2314 2 0.11129541010179622 2314 3 0.1704985055353916 2314 4 0.04909284154493321 2314 5 0.1598202463400753 2314 6 0.23902936183294798 2314 7 0.09036835818557165 2314 8 0.20898001541922184 2314 9 0.3881947803079626 2314 10-11 0.26667484826378285 2314 12-13 0.16879429489105036 2314 14-15 0.191047924928597 2314 16-17 0.42050092823204466 2314 18-19 0.48703654453826317 2314 20-21 0.4960226937473422 2314 22-23 0.21675141542149845 2314 24-25 0.3643214862181665 2314 26-27 0.43770317672611725 2314 28-29 0.574118339778515 2314 30-31 0.4934272884944093 2314 32-33 0.4156142767161626 2314 34-35 0.30257858989599384 2314 36-37 0.41585947288157854 2314 38-39 0.42636734239223273 2314 40-41 0.185541528671763 2314 42-43 0.2283634388596596 2314 44-45 0.4546729269827736 2314 46-47 0.5340465350712407 2314 48-49 -6.395098037461047 2314 50-51 -1.6127780748168306 2314 52-53 -0.7888552239953057 2314 54-55 -0.4024687701303904 2314 56-57 -0.18421547322581944 2314 58-59 0.3158573237449005 2314 60-61 0.5641409247871323 2314 62-63 0.4014587865526451 2314 64-65 0.5504347878584639 2314 66-67 0.5836554881042844 2314 68-69 0.6063111941634745 2314 70-71 0.3953979348252332 2314 72-73 0.577319387882099 2314 74-75 0.6286361957902002 2314 76-77 0.6173272218357688 2314 78-79 0.4208544938806895 2314 80-81 0.48289554744169294 2314 82-83 0.47858975150623806 2314 84-85 0.5143087984299086 2314 86-87 0.6030930343273049 2314 88-89 0.46164276361852075 2314 90-91 0.5819916179787974 2314 92-93 0.6505470684473131 2314 94-95 0.7061402499403044 2314 96-97 0.7419794574317358 2314 98-99 0.7462412589526082 2314 100 -0.6321489447614894 2315 1 0.09958784099024598 2315 2 0.14685301733235434 2315 3 0.28458743851910384 2315 4 0.10343392870855439 2315 5 0.19131522168118664 2315 6 0.2460317845461617 2315 7 0.3138812917172942 2315 8 0.21800258950013784 2315 9 0.40080392745223037 2315 10-11 0.1691528072125834 2315 12-13 0.3928575947628161 2315 14-15 0.251378596361846 2315 16-17 0.33198264232640184 2315 18-19 0.39118688774177457 2315 20-21 0.28234393912374145 2315 22-23 0.386332307922828 2315 24-25 0.4102732484331142 2315 26-27 0.4865784433592353 2315 28-29 0.5262038606908774 2315 30-31 0.5297287340926786 2315 32-33 0.5775539939294205 2315 34-35 0.5764706396637607 2315 36-37 0.4932230311849253 2315 38-39 0.5634598550421046 2315 40-41 0.5838561569232041 2315 42-43 0.6172074402617298 2315 44-45 0.5885638489390317 2315 46-47 0.49670270274628336 2315 48-49 -4.883090273096954 2315 50-51 -1.0048786067237643 2315 52-53 0.13482335842888205 2315 54-55 0.05312749618274637 2315 56-57 0.4362486729335018 2315 58-59 0.5546844584356734 2315 60-61 0.35817464420667733 2315 62-63 0.11080425029353336 2315 64-65 0.33292540062533504 2315 66-67 -0.24774138718921535 2315 68-69 0.4200641963781564 2315 70-71 0.370239251178063 2315 72-73 0.48786349512057114 2315 74-75 0.6345945476286587 2315 76-77 0.5890987809651627 2315 78-79 0.4881790032874136 2315 80-81 0.3822725545819168 2315 82-83 0.3612109015169658 2315 84-85 0.40940531304449834 2315 86-87 0.43222455386471736 2315 88-89 0.40051356000546434 2315 90-91 0.4568979474929016 2315 92-93 0.49571514358529356 2315 94-95 0.125309049703489 2315 96-97 0.2581960118754836 2315 98-99 0.21629087130524738 2315 100 0.20246907185077134 2316 1 0.22768162721476415 2316 2 0.25274761671549584 2316 3 0.18919968426932599 2316 4 -0.06492550538797559 2316 5 0.14158350330681913 2316 6 0.12662523482618582 2316 7 0.1991916717681974 2316 8 0.3027016082091336 2316 9 0.43762051097930765 2316 10-11 0.3764698545288141 2316 12-13 0.2972096804817639 2316 14-15 0.413023037956755 2316 16-17 0.37771924403354973 2316 18-19 0.10946248302966666 2316 20-21 0.44284322630651474 2316 22-23 0.5283871724952434 2316 24-25 0.5476185810591616 2316 26-27 0.6081024458090383 2316 28-29 0.6244278127586043 2316 30-31 0.5211793661359039 2316 32-33 0.46783933730824856 2316 34-35 0.4321222093027828 2316 36-37 0.3380075040543815 2316 38-39 0.45157571112284245 2316 40-41 0.08812629972619845 2316 42-43 0.05728460053217077 2316 44-45 0.2853055812136205 2316 46-47 0.31957394191326927 2316 48-49 -5.153318208717046 2316 50-51 -1.121868681085033 2316 52-53 0.13132736433484382 2316 54-55 0.4325851049829694 2316 56-57 0.3206925723799685 2316 58-59 0.21610014712702252 2316 60-61 0.3764510048971843 2316 62-63 0.2596196151971455 2316 64-65 0.3096478511588643 2316 66-67 0.40635012217065736 2316 68-69 0.10757974942350046 2316 70-71 -0.30774297200975553 2316 72-73 -0.2236714810891769 2316 74-75 -0.036728195564087684 2316 76-77 0.03892435563521701 2316 78-79 0.11570377831348821 2316 80-81 0.024575540907534332 2316 82-83 -0.037518142623902406 2316 84-85 0.10926687765043397 2316 86-87 0.17110221933932124 2316 88-89 -0.020611485134075735 2316 90-91 0.02105738361342091 2316 92-93 0.09447409287131592 2316 94-95 0.30845439075856973 2316 96-97 0.375779077915702 2316 98-99 0.331174464775124 2316 100 -0.4159718279860165 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 1.0 9 2.0 10 10.0 11 15.0 12 46.0 13 254.0 14 1023.0 15 3258.0 16 8287.0 17 18094.0 18 31978.0 19 48191.0 20 70842.0 21 102645.0 22 121486.0 23 110342.0 24 98043.0 25 98013.0 26 111310.0 27 135263.0 28 164531.0 29 201860.0 30 248546.0 31 309128.0 32 392086.0 33 518109.0 34 745621.0 35 1037462.0 36 1624598.0 37 2316443.0 38 1934978.0 39 324524.0 40 27.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.69942813905584 22.995314640469964 14.42195784151012 21.883299378964082 2 14.533411352666686 21.357068729762112 42.11334776431183 21.996172153259366 3 17.482383099058374 29.760112476476543 30.036209490035688 22.721294934429395 4 11.267926947728611 18.10985313179677 41.640526053276915 28.9816938671977 5 11.515429493183513 40.286934402082665 35.187981617818316 13.009654486915508 6 26.887993340639625 40.83689809725535 18.708907321334273 13.566201240770749 7 25.508591806337417 33.74427902650567 24.27990244420253 16.46722672295438 8 25.88852633407469 35.35844488358489 20.503752201063637 18.24927658127678 9 26.83978839419793 15.355396298735899 22.69170256626694 35.113112740799224 10-11 24.410625695063374 27.6351166082624 27.69954611230786 20.25471158436637 12-13 25.014268811889316 25.73020082303045 28.61910748639626 20.63642287868397 14-15 22.810026387672565 26.521805414901102 26.67823574682458 23.989932450601753 16-17 20.047538346535543 29.4336291118183 28.1432912955981 22.37554124604806 18-19 19.53293461124457 28.594828242536767 31.652693021123152 20.21954412509551 20-21 21.26322903057931 27.606134856439983 31.05095444621761 20.07968166676309 22-23 21.632606363085568 27.552242446950743 30.49048442428977 20.324666765673918 24-25 20.86790072502444 27.801990829372436 31.355687882434246 19.974420563168877 26-27 20.592401843591297 28.048849175128275 30.67910122372543 20.679647757554996 28-29 19.932168887324227 27.925529323656967 31.40868818371272 20.733613605306083 30-31 20.78545185211167 27.703601817599843 31.15627412168588 20.35467220860261 32-33 21.068952507760212 28.395603343200822 30.25726569803221 20.278178451006756 34-35 20.91309775871052 28.007937478735418 31.119100417839217 19.95986434471485 36-37 20.859666802417678 27.539906714180756 30.67671570656546 20.9237107768361 38-39 20.897397910801754 27.71472450644252 31.307935737466657 20.07994184528907 40-41 20.38039680750076 26.935798324318355 32.074196562709076 20.60960830547181 42-43 20.494409441753344 26.99471339092113 31.111881410550556 21.398995756774966 44-45 20.368631170565298 28.05760953583224 30.746990878875273 20.826768414727187 46-47 20.62629773407493 27.74668611341193 30.09047116751511 21.53654498499803 48-49 21.481224141564752 27.66848814489477 29.88011340988914 20.970174303651334 50-51 21.241697497109993 27.497632496660806 29.378795337085702 21.881874669143496 52-53 20.42031148999279 28.30879114912921 29.410639550774313 21.860257810103683 54-55 20.78191845699831 28.33217164503693 28.98643678261185 21.89947311535291 56-57 21.386474668143503 27.78714358705866 28.760975349924102 22.065406394873737 58-59 20.271147081924667 27.984151845038447 29.815145380388387 21.929555692648503 60-61 20.60812202732026 28.960929885031018 28.66758712870038 21.763360958948347 62-63 20.990570896446894 28.096645295516698 28.72264460325105 22.190139204785357 64-65 20.083636846913702 28.071499582920183 28.968564580921097 22.87629898924502 66-67 20.7540381858609 27.642185265195778 28.790832430642304 22.812944118301015 68-69 20.18519646941704 27.934680251905515 28.966024666409528 22.914098612267917 70-71 20.35791511120306 27.38324590623141 29.281582095018628 22.977256887546908 72-73 20.929826344368198 27.65647731654924 28.945364906072076 22.468331433010487 74-75 20.877038919961578 28.102395632066003 28.67945182190736 22.341113626065066 76-77 20.421967997959804 27.97558205986615 28.804671327513553 22.79777861466049 78-79 20.298470343549216 27.809671623231473 28.734068060258277 23.15778997296103 80-81 19.965482082585037 28.48972535433316 28.79136624290763 22.75342632017417 82-83 20.956242683023593 27.36979088122033 29.63685060166833 22.037115834087746 84-85 20.92429730736118 27.663704525128612 28.477767510653944 22.934230656856265 86-87 19.751109984168142 28.363039954232583 29.16970548364385 22.716144577955426 88-89 20.63450205520211 27.26158323566215 28.985496955323175 23.118417753812565 90-91 20.394784590137583 27.289187454906195 29.222942464993658 23.09308548996256 92-93 20.694710664980942 27.69310457966553 28.74776247532673 22.864422280026798 94-95 19.447654555052225 27.19339690597355 29.476021018938674 23.88292752003555 96-97 20.03394483715739 27.19293150104272 29.099172447864362 23.673951213935524 98-99 19.445259965657623 27.77450873257331 28.98027366711004 23.799957634659023 100 19.868159657935788 27.198380200018903 28.387091204808385 24.546368937236927 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4425.0 1 2988.0 2 2102.0 3 2548.0 4 2036.5 5 1933.5 6 3181.0 7 6058.0 8 9344.0 9 10794.0 10 9637.5 11 8459.5 12 9173.0 13 10588.5 14 12261.0 15 14128.0 16 16049.0 17 17784.0 18 19466.0 19 21182.5 20 22890.0 21 24759.0 22 26691.5 23 29173.5 24 31788.0 25 35103.5 26 41673.0 27 49392.0 28 56018.5 29 73716.5 30 106552.5 31 140044.5 32 192089.0 33 260989.0 34 325554.0 35 382919.0 36 446647.5 37 522238.0 38 533469.0 39 490721.0 40 432123.0 41 370583.5 42 340995.5 43 339455.5 44 352342.0 45 395614.5 46 421820.5 47 417912.0 48 421594.0 49 405180.5 50 377574.0 51 360412.0 52 343333.5 53 317878.0 54 298657.5 55 257727.0 56 219888.5 57 175411.5 58 129194.0 59 122402.5 60 99338.0 61 63312.5 62 43994.0 63 35908.0 64 24947.5 65 10257.0 66 4978.5 67 3677.5 68 3025.5 69 2505.5 70 2029.5 71 1722.0 72 1541.0 73 1350.5 74 1115.0 75 865.0 76 629.5 77 432.0 78 304.0 79 226.0 80 142.0 81 88.0 82 62.5 83 36.5 84 23.5 85 16.5 86 7.5 87 7.0 88 8.0 89 4.0 90 2.0 91 1.5 92 1.5 93 2.0 94 1.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.030101093837005172 2 0.001865080105190518 3 7.423204398768232E-5 4 0.024079019268504448 5 5.47461324409157E-4 6 5.567403299076173E-5 7 4.6395027492301444E-5 8 0.0037951132488702583 9 0.010494555218758587 10-11 0.0011320386708121553 12-13 1.4846408797536463E-4 14-15 1.3918508247690432E-4 16-17 3.062071814491895E-4 18-19 3.2476519244611004E-5 20-21 7.887154673691245E-5 22-23 2.644516567061182E-4 24-25 9.27900549846029E-6 26-27 7.423204398768232E-5 28-29 9.650165718398699E-4 30-31 0.00257492402582273 32-33 0.009803269309123295 34-35 0.019105472321329735 36-37 0.02216754413582163 38-39 0.021429863198694034 40-41 0.004704455787719366 42-43 0.005047778991162397 44-45 0.009172296935227996 46-47 0.011724023447304574 48-49 1.202753971715921 50-51 0.018121897738492942 52-53 0.018854939172871305 54-55 0.009594491685407939 56-57 0.007636621525232818 58-59 0.008100571800155831 60-61 0.005057057996660857 62-63 0.007488157437257453 64-65 0.019495190552265066 66-67 0.029808805163803673 68-69 0.02983664218029906 70-71 0.02689055793453791 72-73 0.02349444192210145 74-75 0.009557375663414096 76-77 0.014651549682068794 78-79 0.008392860473357331 80-81 0.002737306622045785 82-83 0.01449380658859497 84-85 0.02417180932348905 86-87 0.02355939496059067 88-89 0.033627115926420086 90-91 0.02092415739902795 92-93 0.013338570404036664 94-95 0.0068247085441175415 96-97 0.0038507872818610194 98-99 0.01576966984463326 100 0.03558498608659521 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1.0777017E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.457924182786506 #Duplication Level Percentage of deduplicated Percentage of total 1 71.21655807778517 33.08573455736755 2 15.778553204941554 14.660776570184755 3 5.933617690920759 8.269906824433122 4 2.7281957272652946 5.069852410123726 5 1.4574150248337696 3.385423836329059 6 0.8706769899669524 2.4269907352548867 7 0.5179020340596667 1.6842457402718436 8 0.34185181507665763 1.2705380565263449 9 0.22170377193538415 0.9269907324850681 >10 0.8123314316525458 6.03218367213084 >50 0.04154040805853908 1.3734612036255835 >100 0.056920488123307265 6.009474937844792 >500 0.012211860796840932 3.9777226695152232 >1k 0.01010751492783059 8.561314588322555 >5k 2.414764658392272E-4 0.7348114448935364 >10k+ 1.7248318988516228E-4 2.530572020691154 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 59262 0.5498924238497536 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 44913 0.4167479739523469 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 29596 0.27462144673243066 No Hit TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC 13711 0.127224444389389 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 1.8558010996920575E-5 0.0 0.0 0.0 0.0 7 1.8558010996920575E-5 0.0 0.0 0.0 0.0 8 1.8558010996920575E-5 0.0 0.0 0.0 0.0 9 1.8558010996920575E-5 0.0 0.0 0.0 0.0 10-11 1.8558010996920575E-5 0.0 0.0 9.279005498460288E-6 0.0 12-13 1.8558010996920575E-5 0.0 0.0 9.279005498460288E-6 0.0 14-15 1.8558010996920575E-5 0.0 0.0 1.3918508247690432E-5 0.0 16-17 1.8558010996920575E-5 0.0 0.0 4.639502749230144E-5 0.0 18-19 1.8558010996920575E-5 0.0 0.0 6.959254123845216E-5 0.0 20-21 1.8558010996920575E-5 0.0 0.0 1.670220989722852E-4 0.0 22-23 1.8558010996920575E-5 0.0 0.0 7.979944728675847E-4 0.0 24-25 1.8558010996920575E-5 0.0 0.0 0.0021712872866397077 0.0 26-27 1.8558010996920575E-5 0.0 0.0 0.004750850815211668 0.0 28-29 1.8558010996920575E-5 0.0 0.0 0.011222957150387718 0.0 30-31 1.8558010996920575E-5 0.0 0.0 0.03306573609376324 0.0 32-33 1.8558010996920575E-5 0.0 0.0 0.08074126634485221 0.0 34-35 1.8558010996920575E-5 0.0 0.0 0.14908114184101223 0.0 36-37 1.8558010996920575E-5 0.0 0.0 0.2569727782743592 0.0 38-39 1.8558010996920575E-5 0.0 0.0 0.41773618803793294 0.0 40-41 1.8558010996920575E-5 0.0 0.0 0.5941068850499169 0.0 42-43 1.8558010996920575E-5 0.0 0.0 0.7652952574910108 0.0 44-45 1.8558010996920575E-5 0.0 0.0 0.9601172569366829 0.0 46-47 1.8558010996920575E-5 0.0 0.0 1.191201609870338 0.0 48-49 1.8558010996920575E-5 0.0 0.0 1.4545212279056439 0.0 50-51 1.8558010996920575E-5 4.639502749230144E-6 0.0 1.7451860751449124 0.0 52-53 1.8558010996920575E-5 9.279005498460288E-6 0.0 2.0192739790611816 0.0 54-55 1.8558010996920575E-5 9.279005498460288E-6 0.0 2.304733304215814 0.0 56-57 1.8558010996920575E-5 1.3918508247690432E-5 0.0 2.632741509083636 0.0 58-59 1.8558010996920575E-5 2.7837016495380865E-5 0.0 3.020409079803809 0.0 60-61 2.7837016495380865E-5 2.7837016495380865E-5 0.0 3.4196058148558177 0.0 62-63 3.711602199384115E-5 2.7837016495380865E-5 0.0 3.8227414877419235 0.0 64-65 3.711602199384115E-5 2.7837016495380865E-5 0.0 4.218829755951948 0.0 66-67 3.711602199384115E-5 2.7837016495380865E-5 0.0 4.648109026829966 0.0 68-69 3.711602199384115E-5 2.7837016495380865E-5 0.0 5.114481122188078 0.0 70-71 3.711602199384115E-5 2.7837016495380865E-5 0.0 5.62635746051064 0.0 72-73 3.711602199384115E-5 2.7837016495380865E-5 0.0 6.149813997695281 0.0 74-75 3.711602199384115E-5 2.7837016495380865E-5 0.0 6.636896833325957 0.0 76-77 3.711602199384115E-5 2.7837016495380865E-5 0.0 7.163424721330587 0.0 78-79 3.711602199384115E-5 2.7837016495380865E-5 0.0 7.721515146538231 0.0 80-81 3.711602199384115E-5 2.7837016495380865E-5 0.0 8.330417405855442 9.279005498460288E-6 82-83 3.711602199384115E-5 2.7837016495380865E-5 0.0 8.956592533908038 9.279005498460288E-6 84-85 3.711602199384115E-5 2.7837016495380865E-5 0.0 9.537523231150141 9.279005498460288E-6 86-87 3.711602199384115E-5 2.7837016495380865E-5 0.0 10.127872118973182 9.279005498460288E-6 88 5.567403299076173E-5 2.7837016495380865E-5 0.0 10.585971980929417 9.279005498460288E-6 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 27985 0.0 51.79991 1 GTATCAA 53495 0.0 42.56415 1 TACCCCG 570 0.0 35.389038 5 TACCTGG 3580 0.0 34.205753 2 ACCTGGG 3575 0.0 33.46864 3 ATCAACG 66350 0.0 33.364983 3 CAACGCA 66625 0.0 33.170425 5 TATCAAC 70585 0.0 32.118565 2 TAGGCAT 10060 0.0 31.569365 5 AACGCAG 72770 0.0 30.839685 6 CTGTGCG 10810 0.0 29.422062 9 TCAACGC 75635 0.0 29.399628 4 TATTCTC 6550 0.0 28.36149 5 CTAGGCA 11320 0.0 28.062101 4 TAGTACT 11420 0.0 27.775284 4 ACTGTGC 11690 0.0 27.729156 8 ACGCAGA 79890 0.0 27.62155 7 GGGCACC 10690 0.0 27.56883 94 CGCAGAG 80335 0.0 27.42183 8 CATGGGT 4530 0.0 26.931038 4 >>END_MODULE