FastQCFastQC Report
Mon 6 Jun 2016
ERR1211344_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211344_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9439338
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT336220.35619023283200585No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT245960.26056912041924973No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163170.17286169856403066No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA145950.049.758421
GTATCAA267800.041.6959151
TACCTGG88350.037.689682
ATCAACG327150.033.4094473
CAACGCA329250.033.1667565
TATCAAC340000.032.396922
TCAACGC340850.032.231524
ACCTGGG102650.031.7692553
AACGCAG354050.031.1366736
GTACCTG108850.031.0255431
GTACATG351500.028.3683381
ACGCAGA395600.027.5695697
TACATGG359400.027.2064672
CGCAGAG408700.026.6728328
ACATGGG367050.025.7450263
CATGGGG257050.023.9471194
GCAGAGT448300.023.688979
GTGGTAT74350.023.1538621
ATGGGGG173400.022.7626555
TGGTATC77100.020.6185462
Produced by FastQC (version 0.11.5)