FastQCFastQC Report
Mon 6 Jun 2016
ERR1211344_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211344_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9439338
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT291180.3084750222949957No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT225350.23873496213399711No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156120.16539295446354396No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA144800.049.745461
GTATCAA258300.040.458471
TACCTGG85250.038.9078642
GTACCTG109400.031.3126031
ATCAACG327750.031.2782023
CAACGCA331250.030.9134925
TATCAAC340100.030.4720342
TCAACGC338150.030.3302614
ACCTGGG108750.029.8545073
AACGCAG355300.029.2170546
GTACATG356050.027.8221631
TACATGG366550.026.4685212
ACGCAGA404250.025.505157
ACATGGG364550.025.4824493
CGCAGAG417100.024.6856488
CATGGGG257800.023.7135684
ATGGGGG166050.023.5601145
TGGGGGG152700.022.7937156
GTGGTAT78550.021.8649541
CCTGGGG110250.021.703364
Produced by FastQC (version 0.11.5)