FastQCFastQC Report
Mon 6 Jun 2016
ERR1211343_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211343_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6572475
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT324270.49337578309540925No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT255790.3891836789033051No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149540.227524638739592No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85520.13011841049224226No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA131650.052.56271
TACCTGG52800.051.6713872
ACCTGGG63750.042.67873
GTATCAA246600.041.6052781
GTACCTG69400.039.8569151
ATCAACG295750.033.8905753
CAACGCA296650.033.8033375
TCAACGC308000.032.6797144
AACGCAG315750.032.0261766
TATCAAC319300.031.5114142
TATCACG5000.031.0455952
ACGCAGA353600.028.2529537
CGCAGAG356650.027.864078
GTACATG296450.027.5158841
CCTGGGG53450.026.9019184
GCAGAGT385300.025.427489
TACATGG314650.025.3693982
ACATGGG302000.025.2847843
CATGGGG151850.022.9304664
GAGTACT229900.022.3808412-13
Produced by FastQC (version 0.11.5)