##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211343_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6572475 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.416628439058346 31.0 31.0 34.0 28.0 34.0 2 31.43317760813088 31.0 31.0 34.0 28.0 34.0 3 31.475150380944772 33.0 31.0 34.0 28.0 34.0 4 35.09311834582863 37.0 35.0 37.0 32.0 37.0 5 35.15155295379594 37.0 35.0 37.0 32.0 37.0 6 35.220041765088496 37.0 35.0 37.0 32.0 37.0 7 35.164112301682394 37.0 35.0 37.0 32.0 37.0 8 35.16392804841403 37.0 35.0 37.0 32.0 37.0 9 36.72420633018764 39.0 37.0 39.0 32.0 39.0 10-11 36.65693053225763 39.0 37.0 39.0 32.0 39.0 12-13 36.59260894868372 39.0 37.0 39.0 32.0 39.0 14-15 37.716572067600104 40.0 37.0 41.0 32.0 41.0 16-17 37.71992742460032 40.0 37.0 41.0 32.0 41.0 18-19 37.759772840520505 40.0 37.5 41.0 32.0 41.0 20-21 37.73839383185178 40.0 37.0 41.0 32.0 41.0 22-23 37.61045603064295 40.0 37.0 41.0 32.0 41.0 24-25 37.50863419031643 40.0 37.0 41.0 32.0 41.0 26-27 37.36465874727557 40.0 37.0 41.0 31.0 41.0 28-29 37.23906313831547 40.0 36.0 41.0 31.0 41.0 30-31 37.051973647674586 39.5 36.0 41.0 30.5 41.0 32-33 36.907983141814924 39.0 36.0 41.0 30.0 41.0 34-35 36.76133161404189 39.0 35.5 41.0 30.0 41.0 36-37 36.660340739219244 39.0 35.0 41.0 30.0 41.0 38-39 36.51354177840159 39.0 35.0 41.0 30.0 41.0 40-41 36.36902666042853 39.0 35.0 41.0 29.0 41.0 42-43 36.22463097995808 39.0 35.0 40.5 28.5 41.0 44-45 36.08419476681159 39.0 35.0 40.5 28.0 41.0 46-47 35.90372028801936 39.0 35.0 41.0 27.0 41.0 48-49 35.659910992434355 39.0 35.0 40.0 26.0 41.0 50-51 35.00131685552246 38.0 33.5 39.5 25.0 40.5 52-53 35.24634814130141 38.0 34.5 40.0 26.0 40.5 54-55 35.61942677606229 39.0 35.0 40.0 26.0 41.0 56-57 35.54847884548819 39.0 34.5 40.5 26.0 41.0 58-59 35.36015983324394 38.0 34.0 40.0 26.0 41.0 60-61 35.126486369290106 38.0 34.0 40.0 26.0 41.0 62-63 34.87497441983423 38.0 34.0 40.0 25.5 41.0 64-65 34.631445460043594 37.0 34.0 40.0 25.0 41.0 66-67 34.42751490115976 37.0 33.5 40.0 25.0 41.0 68-69 34.09743871220506 36.0 33.0 39.5 24.0 41.0 70-71 33.76708568689877 36.0 33.0 39.0 23.5 41.0 72-73 33.40439956028741 35.5 32.5 39.0 23.0 40.5 74-75 33.022119977634 35.0 32.0 38.0 22.5 40.0 76-77 32.65877960737774 35.0 32.0 37.0 22.0 39.0 78-79 32.27664083925766 35.0 31.5 37.0 21.0 39.0 80-81 31.92429533775328 35.0 31.0 36.0 20.0 39.0 82-83 31.587417981810503 35.0 31.0 36.0 20.0 37.0 84-85 31.25214671794111 34.5 31.0 35.0 20.0 37.0 86-87 30.994274455209037 34.0 30.0 35.0 19.0 36.5 88-89 30.720231876119726 34.0 30.0 35.0 18.0 36.0 90-91 30.449979802129334 34.0 29.5 35.0 18.0 36.0 92-93 30.21184721128646 34.0 29.0 35.0 17.5 36.0 94-95 29.987345558560513 34.0 29.0 35.0 16.5 35.0 96-97 29.790026512082587 34.0 29.0 35.0 15.0 35.0 98-99 29.56082967223154 34.0 29.0 35.0 13.0 35.0 100 27.927282492516138 32.0 25.0 35.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.44963880555224023 1101 2 -0.41932636539488755 1101 3 -0.4056626961140637 1101 4 -0.31981689309122174 1101 5 -0.33912097724482493 1101 6 -0.41707032064149274 1101 7 -0.5011773363713061 1101 8 -0.5367161784964765 1101 9 -0.5234555088039912 1101 10-11 -0.5651068346547774 1101 12-13 -0.465760834299104 1101 14-15 -0.5898248879305825 1101 16-17 -0.5692302095820736 1101 18-19 -0.6106272774737747 1101 20-21 -0.7029608487745946 1101 22-23 -0.6813738081074803 1101 24-25 -0.6425505568378611 1101 26-27 -0.7680146146423041 1101 28-29 -0.7988779173587162 1101 30-31 -0.7496278486244634 1101 32-33 -0.7821917712053903 1101 34-35 -0.6918937182267513 1101 36-37 -0.840845012436418 1101 38-39 -0.8672594923462142 1101 40-41 -0.8182409658510181 1101 42-43 -0.8536245483671152 1101 44-45 -1.0268866835365316 1101 46-47 -0.8865863406012053 1101 48-49 -0.8683093905274575 1101 50-51 -0.8396357217034236 1101 52-53 -0.67625883230167 1101 54-55 -0.592632338404961 1101 56-57 -0.5494719393151755 1101 58-59 -0.5804865670777204 1101 60-61 -0.62822960608095 1101 62-63 -0.603472915954427 1101 64-65 -0.6225862891729506 1101 66-67 -0.6951270689854425 1101 68-69 -0.6398013473873192 1101 70-71 -0.5247447546869353 1101 72-73 -0.6031997644156206 1101 74-75 -0.653886705800538 1101 76-77 -0.6923884108503344 1101 78-79 -0.6412623676749405 1101 80-81 -0.6901925878168207 1101 82-83 -0.7128196531410893 1101 84-85 -0.7481816241376222 1101 86-87 -0.797820276498058 1101 88-89 -0.8191323346938653 1101 90-91 -0.8862037949620003 1101 92-93 -0.9885697374030009 1101 94-95 -0.8463134349710906 1101 96-97 -0.9440060817089666 1101 98-99 -0.8906745682157506 1101 100 -0.9868074147402588 1102 1 -0.342519207126184 1102 2 -0.36110915305573243 1102 3 -0.3310627733547129 1102 4 -0.20528805831861519 1102 5 -0.2514315746214635 1102 6 -0.24088345389471755 1102 7 -0.32199574072298276 1102 8 -0.25876005943358393 1102 9 -0.362996300905607 1102 10-11 -0.3275191842790406 1102 12-13 -0.3591432129826728 1102 14-15 -0.4824831314792135 1102 16-17 -0.6265522880273764 1102 18-19 -0.5031366735320972 1102 20-21 -0.48891343060417114 1102 22-23 -0.4975287008774316 1102 24-25 -0.5208338370700005 1102 26-27 -0.6233507132756912 1102 28-29 -0.5950304727100288 1102 30-31 -0.517037882610289 1102 32-33 -0.5213207336325496 1102 34-35 -0.5603620665807298 1102 36-37 -0.5524822177007138 1102 38-39 -0.7780547687083796 1102 40-41 -0.7211173814872893 1102 42-43 -0.6931274590567185 1102 44-45 -0.7591216323141836 1102 46-47 -0.7320518468490036 1102 48-49 -0.758055863530835 1102 50-51 -0.6547372111366556 1102 52-53 -0.480164089664072 1102 54-55 -0.54061672300287 1102 56-57 -0.4327076817632616 1102 58-59 -0.426824297677193 1102 60-61 -0.46650951828770815 1102 62-63 -0.5322441323281737 1102 64-65 -0.5457658348367147 1102 66-67 -0.46113449045096644 1102 68-69 -0.47600891403480716 1102 70-71 -0.5594903827799484 1102 72-73 -0.6268639985514 1102 74-75 -0.5063222143906003 1102 76-77 -0.5507916293118953 1102 78-79 -0.49858128718478056 1102 80-81 -0.6316033212858159 1102 82-83 -0.5965652942376849 1102 84-85 -0.6410802242774736 1102 86-87 -0.6491614833785491 1102 88-89 -0.6836605644609577 1102 90-91 -0.6607156095581068 1102 92-93 -0.6973966033134182 1102 94-95 -0.6385672379792986 1102 96-97 -0.7435071086533931 1102 98-99 -0.7848980981677016 1102 100 -0.612544097689586 1103 1 -0.4988372553710789 1103 2 -0.5132963376660165 1103 3 -0.46345211254809016 1103 4 -0.3472241046789364 1103 5 -0.36288127461390474 1103 6 -0.3646406294860256 1103 7 -0.4425098065845816 1103 8 -0.4379594397737776 1103 9 -0.5448588473968954 1103 10-11 -0.5633394188436966 1103 12-13 -0.6559649845602706 1103 14-15 -0.7750204050225733 1103 16-17 -0.7841664174738057 1103 18-19 -0.7431905981551736 1103 20-21 -0.8645412555236405 1103 22-23 -0.705088934051318 1103 24-25 -0.7590136004029375 1103 26-27 -0.8014299290579245 1103 28-29 -0.7136212416685126 1103 30-31 -0.7203639956896026 1103 32-33 -0.7805042593359204 1103 34-35 -0.9404015528194023 1103 36-37 -1.0451277870907276 1103 38-39 -0.9215240851028383 1103 40-41 -0.8950883981332254 1103 42-43 -0.9266404062827789 1103 44-45 -0.8814885328578725 1103 46-47 -0.933129141580082 1103 48-49 -0.9067156685830184 1103 50-51 -0.9461266863170223 1103 52-53 -0.8887537720645327 1103 54-55 -0.8546067485212134 1103 56-57 -0.7625265737868787 1103 58-59 -0.7229223980354575 1103 60-61 -0.6706410572383561 1103 62-63 -0.8417565895041434 1103 64-65 -0.7163689357032297 1103 66-67 -0.777721806951746 1103 68-69 -0.8085584241774342 1103 70-71 -0.7219441710146555 1103 72-73 -0.7741183976540711 1103 74-75 -0.7657699893500691 1103 76-77 -0.7220860531222115 1103 78-79 -0.754351313275869 1103 80-81 -0.7980200519986731 1103 82-83 -0.8301970270072303 1103 84-85 -0.7539056417971928 1103 86-87 -0.9444823172702641 1103 88-89 -0.9691580664446171 1103 90-91 -0.8603962424082354 1103 92-93 -0.8262677211261575 1103 94-95 -1.0320728287201284 1103 96-97 -1.0084859927630738 1103 98-99 -1.0199212208934298 1103 100 -1.016699693181522 1104 1 -0.3541279168468847 1104 2 -0.3449283159220293 1104 3 -0.39295455379469857 1104 4 -0.2394674963636234 1104 5 -0.221966159083145 1104 6 -0.29520335547640286 1104 7 -0.2719216539585716 1104 8 -0.2917338964995224 1104 9 -0.35439321521154454 1104 10-11 -0.4111858660319925 1104 12-13 -0.4156051770419822 1104 14-15 -0.5207665661140624 1104 16-17 -0.5628743204644451 1104 18-19 -0.6086365827069429 1104 20-21 -0.5914372323915771 1104 22-23 -0.4845864078748576 1104 24-25 -0.494789507628397 1104 26-27 -0.5835735214715285 1104 28-29 -0.574352022304069 1104 30-31 -0.6799944358391841 1104 32-33 -0.6857654174214076 1104 34-35 -0.6604349419330688 1104 36-37 -0.6224932569237751 1104 38-39 -0.6203019682250002 1104 40-41 -0.7165991834338357 1104 42-43 -0.63701176207109 1104 44-45 -0.6567983899408887 1104 46-47 -0.7021249263581026 1104 48-49 -0.5942518291719949 1104 50-51 -0.5875461793247325 1104 52-53 -0.5555868509530555 1104 54-55 -0.5467485741991638 1104 56-57 -0.4733641312042707 1104 58-59 -0.5580403635347153 1104 60-61 -0.4488164299654329 1104 62-63 -0.49685089936207305 1104 64-65 -0.5295915467073371 1104 66-67 -0.538667506964174 1104 68-69 -0.5109218559373261 1104 70-71 -0.5408485497326936 1104 72-73 -0.5578806764364401 1104 74-75 -0.5830383732870317 1104 76-77 -0.534122368665578 1104 78-79 -0.5835536497716021 1104 80-81 -0.6960906750662268 1104 82-83 -0.7582842393818723 1104 84-85 -0.7703582306793884 1104 86-87 -0.6802633044690936 1104 88-89 -0.5741921892646253 1104 90-91 -0.49066952854993673 1104 92-93 -0.6553911051014509 1104 94-95 -0.713643686074839 1104 96-97 -0.7362408653701173 1104 98-99 -0.7572088156470969 1104 100 -0.8566582953529256 1105 1 -0.3510860377790941 1105 2 -0.27524649164991644 1105 3 -0.3030850211071261 1105 4 -0.1481140467130686 1105 5 -0.14158441372427433 1105 6 -0.2471458973562619 1105 7 -0.19758664416436744 1105 8 -0.2000639635814636 1105 9 -0.26876455830852564 1105 10-11 -0.31625700851326854 1105 12-13 -0.4033271901963076 1105 14-15 -0.46916361390810124 1105 16-17 -0.4406279523729637 1105 18-19 -0.43820792616788395 1105 20-21 -0.45694896166524757 1105 22-23 -0.5766127185819201 1105 24-25 -0.4657861904970275 1105 26-27 -0.495638745118562 1105 28-29 -0.5186347876205488 1105 30-31 -0.5552326655620163 1105 32-33 -0.5056063363819021 1105 34-35 -0.48288219870274673 1105 36-37 -0.5875215999481895 1105 38-39 -0.5283788771280911 1105 40-41 -0.640187156227995 1105 42-43 -0.6217214118478083 1105 44-45 -0.6376787627917224 1105 46-47 -0.6457253332765305 1105 48-49 -0.6632956823219303 1105 50-51 -0.5675810044269198 1105 52-53 -0.5680369557877825 1105 54-55 -0.44131434891948373 1105 56-57 -0.4717787291755471 1105 58-59 -0.3665480820867444 1105 60-61 -0.37156110138812437 1105 62-63 -0.41147193154907313 1105 64-65 -0.41448137868287205 1105 66-67 -0.4630369915394681 1105 68-69 -0.3840819308423562 1105 70-71 -0.35113434691907486 1105 72-73 -0.40839891849785914 1105 74-75 -0.4496707604273311 1105 76-77 -0.418432891242297 1105 78-79 -0.34969011644662373 1105 80-81 -0.4009963022012819 1105 82-83 -0.4434967591755843 1105 84-85 -0.4169587681720479 1105 86-87 -0.4483473517333252 1105 88-89 -0.4841029950099802 1105 90-91 -0.539930277095138 1105 92-93 -0.46731928836578973 1105 94-95 -0.5765332675935362 1105 96-97 -0.6966396524856755 1105 98-99 -0.7053193792614287 1105 100 -0.6561458142537724 1106 1 -0.44639092702068695 1106 2 -0.5062034401835867 1106 3 -0.44025586438761977 1106 4 -0.3681897010456794 1106 5 -0.3159875180196323 1106 6 -0.3156125121176103 1106 7 -0.3498266047302607 1106 8 -0.3043490684014998 1106 9 -0.45874765789655925 1106 10-11 -0.49878315740444634 1106 12-13 -0.46527720972400743 1106 14-15 -0.6046575636909708 1106 16-17 -0.5520578764573614 1106 18-19 -0.6795339845534514 1106 20-21 -0.5493909815766003 1106 22-23 -0.5157680469032826 1106 24-25 -0.5727006806154975 1106 26-27 -0.4979631908882638 1106 28-29 -0.5304750912852043 1106 30-31 -0.5657857589206188 1106 32-33 -0.6881837416340488 1106 34-35 -0.6129625131642413 1106 36-37 -0.609708707325737 1106 38-39 -0.6328522637410714 1106 40-41 -0.6533895390405533 1106 42-43 -0.6235331816342935 1106 44-45 -0.6146060258436421 1106 46-47 -0.7036201725855236 1106 48-49 -0.6984650609262815 1106 50-51 -0.6432721955415914 1106 52-53 -0.5984238307656966 1106 54-55 -0.6567860751578749 1106 56-57 -0.5253534677484382 1106 58-59 -0.5897340662350032 1106 60-61 -0.5516953663651663 1106 62-63 -0.5472335409533002 1106 64-65 -0.5228084036490586 1106 66-67 -0.5452193112441961 1106 68-69 -0.5831867382872744 1106 70-71 -0.5738607094210195 1106 72-73 -0.5394723899191121 1106 74-75 -0.5506982418715225 1106 76-77 -0.5573349886992247 1106 78-79 -0.5532603516516588 1106 80-81 -0.5205037736622096 1106 82-83 -0.49260920239957784 1106 84-85 -0.49225460123357934 1106 86-87 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0.4996308668181726 2311 9 0.5717545565390267 2311 10-11 0.6169752515682845 2311 12-13 0.6527127087347253 2311 14-15 0.8861806450937806 2311 16-17 0.8659454476682882 2311 18-19 0.7467075337428781 2311 20-21 0.7731522155486914 2311 22-23 0.825043143488486 2311 24-25 0.8483416611291261 2311 26-27 0.9112939765327823 2311 28-29 0.9178296677695457 2311 30-31 0.8697172784337326 2311 32-33 0.8920478736218627 2311 34-35 0.9365841777394408 2311 36-37 0.9156496821132833 2311 38-39 0.9696029556401626 2311 40-41 1.0343399331529497 2311 42-43 1.0022830303383543 2311 44-45 0.9649246884650111 2311 46-47 0.9662164115654619 2311 48-49 0.9847002274843959 2311 50-51 1.008419872759326 2311 52-53 0.8925501658385855 2311 54-55 0.8822752864763501 2311 56-57 0.8233863916418684 2311 58-59 0.841191334147851 2311 60-61 0.8297396337899556 2311 62-63 0.8324022055335902 2311 64-65 0.8664877280081598 2311 66-67 0.9149521219811305 2311 68-69 0.842144511691373 2311 70-71 0.7913250678000097 2311 72-73 0.7396121197988279 2311 74-75 0.8364629400240275 2311 76-77 0.8664296832816021 2311 78-79 0.8708170136197495 2311 80-81 0.9218081046525377 2311 82-83 0.9057055102044416 2311 84-85 0.9684573582574068 2311 86-87 0.911787081983988 2311 88-89 0.9399196745669762 2311 90-91 0.9788283078240312 2311 92-93 0.9968279641073714 2311 94-95 1.0144176081575047 2311 96-97 1.0412130459733895 2311 98-99 0.9867833668789956 2311 100 1.0586563665380737 2312 1 0.5550722280737546 2312 2 0.5046553472115143 2312 3 0.5297555022717901 2312 4 0.42960468334088375 2312 5 0.43584444889613394 2312 6 0.5149879860806479 2312 7 0.5287194302403577 2312 8 0.5177506196199815 2312 9 0.5962355339581364 2312 10-11 0.6066607469221381 2312 12-13 0.6819210296529974 2312 14-15 0.8776636532671205 2312 16-17 0.8330179415793495 2312 18-19 0.7636448105806508 2312 20-21 0.8169238023359569 2312 22-23 0.8233937224260899 2312 24-25 0.8411605977709229 2312 26-27 0.9100273288340972 2312 28-29 0.8425750429381154 2312 30-31 0.8903377265324721 2312 32-33 0.9137957500102516 2312 34-35 0.9110223474128034 2312 36-37 0.8867724651330917 2312 38-39 0.933273881994019 2312 40-41 0.8990361544465912 2312 42-43 0.9680833024402844 2312 44-45 0.9165192734380838 2312 46-47 0.9788086648712451 2312 48-49 0.9932203486362212 2312 50-51 0.9786715032177469 2312 52-53 0.9203432494539001 2312 54-55 0.8606750484484991 2312 56-57 0.8347519761558857 2312 58-59 0.8180811167198456 2312 60-61 0.8364458220553459 2312 62-63 0.7497385744545682 2312 64-65 0.7937046921936428 2312 66-67 0.7955567655653937 2312 68-69 0.8435708616546833 2312 70-71 0.8341842251602216 2312 72-73 0.8338437725031937 2312 74-75 0.7826834602252646 2312 76-77 0.8320819258573593 2312 78-79 0.7916693927194274 2312 80-81 0.8002657733643197 2312 82-83 0.8519638223410482 2312 84-85 0.9342309244139635 2312 86-87 0.8099706207450694 2312 88-89 0.8033185772193221 2312 90-91 0.8535879409803542 2312 92-93 0.9319190216020026 2312 94-95 0.8872429272002051 2312 96-97 0.847269083154611 2312 98-99 0.9128178017596795 2312 100 1.011140231044127 2313 1 0.4571433066161106 2313 2 0.469049730098849 2313 3 0.5259422731300667 2313 4 0.40346401239151675 2313 5 0.44579889983486254 2313 6 0.4331743765691414 2313 7 0.4740590981857409 2313 8 0.45120137440164143 2313 9 0.5127739945703453 2313 10-11 0.5626331478278246 2313 12-13 0.5691191883023379 2313 14-15 0.6963328223558705 2313 16-17 0.7015477418085467 2313 18-19 0.6452590736267396 2313 20-21 0.6889751521212446 2313 22-23 0.603189516697654 2313 24-25 0.5680921743209169 2313 26-27 0.6384264445336996 2313 28-29 0.7383145706344365 2313 30-31 0.7916923894050782 2313 32-33 0.7276137373358011 2313 34-35 0.7850187252504455 2313 36-37 0.8024318082059168 2313 38-39 0.8336281831863062 2313 40-41 0.8279141700872046 2313 42-43 0.8056845601231117 2313 44-45 0.8989608526381829 2313 46-47 0.8967949575842837 2313 48-49 0.9356209866968683 2313 50-51 0.8361017661284293 2313 52-53 0.7970055822657969 2313 54-55 0.7839249124240766 2313 56-57 0.7755045486634984 2313 58-59 0.764078927168363 2313 60-61 0.8022404993001828 2313 62-63 0.8239271163693758 2313 64-65 0.7276595200424012 2313 66-67 0.6793973569776668 2313 68-69 0.6863637819194537 2313 70-71 0.7071869742765742 2313 72-73 0.719211592357837 2313 74-75 0.6829753585896654 2313 76-77 0.7510350935314065 2313 78-79 0.7541317386037747 2313 80-81 0.804017066067015 2313 82-83 0.7775731305398494 2313 84-85 0.7264588561401943 2313 86-87 0.7536373518921167 2313 88-89 0.7422375546900959 2313 90-91 0.8696548248044493 2313 92-93 0.7946379390154554 2313 94-95 0.9218357258104142 2313 96-97 0.9787056011707698 2313 98-99 0.9072169126427596 2313 100 1.0206506289206239 2314 1 0.2854718341002709 2314 2 0.19327062610902246 2314 3 0.26619873976526875 2314 4 0.1965744028892331 2314 5 0.1417223930469973 2314 6 0.21674347442647246 2314 7 0.26300855667101786 2314 8 0.27710121304694013 2314 9 0.3317162041434685 2314 10-11 0.36286229668021264 2314 12-13 0.379350548252944 2314 14-15 0.411586072243729 2314 16-17 0.4692075792835908 2314 18-19 0.4315254550767733 2314 20-21 0.45152473195258125 2314 22-23 0.46678366609997113 2314 24-25 0.4886190945633899 2314 26-27 0.54745995978125 2314 28-29 0.5638976971104555 2314 30-31 0.5999243142447312 2314 32-33 0.6584531749209006 2314 34-35 0.6049728925608164 2314 36-37 0.5957541618380731 2314 38-39 0.6082171149871414 2314 40-41 0.5353868403845041 2314 42-43 0.5558566200217214 2314 44-45 0.6975886905561595 2314 46-47 0.4554005181281724 2314 48-49 0.3298179582804792 2314 50-51 0.5708361471294268 2314 52-53 0.5443024927968878 2314 54-55 0.5243156358923642 2314 56-57 0.5506224395748447 2314 58-59 0.5240347885620551 2314 60-61 0.35935497136132 2314 62-63 0.5795051605954313 2314 64-65 0.5829543072445418 2314 66-67 0.593831561528674 2314 68-69 0.5488523741024807 2314 70-71 0.5546442502444293 2314 72-73 0.5942831968130307 2314 74-75 0.6008541505769003 2314 76-77 0.5950756032380369 2314 78-79 0.6061617745745806 2314 80-81 0.5945883993164252 2314 82-83 0.6268429913314861 2314 84-85 0.6444019992917269 2314 86-87 0.6208605063881336 2314 88-89 0.5313466342274538 2314 90-91 0.560302155027415 2314 92-93 0.6881486900872851 2314 94-95 0.755561066608049 2314 96-97 0.7376765637782334 2314 98-99 0.7443123699727678 2314 100 0.6339373182552919 2315 1 0.24978451598978424 2315 2 0.2839148752823917 2315 3 0.3017516708666861 2315 4 0.20095286769161902 2315 5 0.23714146570437578 2315 6 0.21560214498907015 2315 7 0.20154484031723996 2315 8 0.2177459634457719 2315 9 0.11639989963064323 2315 10-11 0.2970757059845752 2315 12-13 0.27693479123455234 2315 14-15 0.38395963128562016 2315 16-17 0.20218257763670522 2315 18-19 0.40243120464057114 2315 20-21 0.2706298583304019 2315 22-23 0.2337106846781296 2315 24-25 0.4139789418992308 2315 26-27 0.5136339782320718 2315 28-29 0.38897157253627057 2315 30-31 0.39882671835639627 2315 32-33 0.4312681118042434 2315 34-35 0.31160756520330324 2315 36-37 0.4449619131531719 2315 38-39 0.40973663205576116 2315 40-41 0.4567318880295588 2315 42-43 0.5065914458439167 2315 44-45 0.5024568819289854 2315 46-47 0.5183442979462072 2315 48-49 0.424037002791124 2315 50-51 0.35972398510836 2315 52-53 0.13760782607015898 2315 54-55 0.30654584900822357 2315 56-57 0.23099817055034322 2315 58-59 0.25278717393960193 2315 60-61 0.37624242892965754 2315 62-63 0.32683899914667336 2315 64-65 0.3324523494709055 2315 66-67 0.27190256669012314 2315 68-69 0.3500468920238262 2315 70-71 0.2596525927191564 2315 72-73 0.323060985208393 2315 74-75 0.03939132926858946 2315 76-77 0.24866239543933233 2315 78-79 0.36244313859523913 2315 80-81 0.35574470172540273 2315 82-83 0.1952897548080017 2315 84-85 0.24665384907406462 2315 86-87 0.19834303054394908 2315 88-89 0.21206772256629236 2315 90-91 0.3714563158366886 2315 92-93 0.4785784310357357 2315 94-95 0.4144641197090486 2315 96-97 0.3731441205605712 2315 98-99 0.5525355497887716 2315 100 0.5147934217623842 2316 1 0.03800459836962844 2316 2 0.0702748283258039 2316 3 0.05231880248984666 2316 4 -0.043864963282928215 2316 5 -0.0041064637691548 2316 6 -0.15982279772695307 2316 7 -0.07095486993446798 2316 8 -0.00659086028783662 2316 9 0.029192482691925647 2316 10-11 0.020206279619294776 2316 12-13 -0.004371610794059677 2316 14-15 -0.16028068729470846 2316 16-17 0.01756506660086643 2316 18-19 0.09238207799528908 2316 20-21 0.14800352711228015 2316 22-23 0.0626545431657064 2316 24-25 0.03373197345098333 2316 26-27 -0.056316677087870914 2316 28-29 -0.051253452222574936 2316 30-31 -0.5955243557832759 2316 32-33 -0.4003794825002984 2316 34-35 -0.5629292475578112 2316 36-37 -0.35879703865393253 2316 38-39 -0.1510508301467226 2316 40-41 0.01339875011285585 2316 42-43 0.03166374945793393 2316 44-45 -0.17105644404819742 2316 46-47 -0.2933468669756891 2316 48-49 -0.2956144608911657 2316 50-51 -0.20167751996194028 2316 52-53 -0.4777223474096317 2316 54-55 -0.412919223877779 2316 56-57 -0.5350570763119791 2316 58-59 -0.7824036801380174 2316 60-61 -0.36990932147801914 2316 62-63 -0.28281820193761575 2316 64-65 -0.6341017339603994 2316 66-67 -0.9768562188574208 2316 68-69 -0.965224521403691 2316 70-71 -0.7239736865307833 2316 72-73 -0.9696940598527704 2316 74-75 -0.8195785718336595 2316 76-77 -0.47284590133083526 2316 78-79 -0.41321011676696884 2316 80-81 -0.4041228193117341 2316 82-83 -0.30018759922478466 2316 84-85 -0.2032616479764222 2316 86-87 -0.08617028366321122 2316 88-89 -0.10420538863826323 2316 90-91 -0.6750585268007292 2316 92-93 -0.7515240379155337 2316 94-95 -0.45990465673249403 2316 96-97 -0.20915687864331645 2316 98-99 -0.11785950897706954 2316 100 -0.2714243102153695 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 182.0 1 489.0 2 307.0 3 259.0 4 288.0 5 222.0 6 284.0 7 686.0 8 1752.0 9 2842.0 10 4555.0 11 7222.0 12 10987.0 13 14462.0 14 17148.0 15 20079.0 16 24584.0 17 30737.0 18 38307.0 19 45819.0 20 54719.0 21 66985.0 22 73709.0 23 72782.0 24 69694.0 25 69776.0 26 75601.0 27 85642.0 28 98838.0 29 116974.0 30 142540.0 31 174764.0 32 220122.0 33 287140.0 34 394871.0 35 556388.0 36 821960.0 37 1264571.0 38 1360858.0 39 343069.0 40 261.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.42159390114672 20.553892222403665 13.72823346409856 22.296280412351052 2 15.393617669459253 21.17850723533892 41.426306169078444 22.001568926123383 3 17.837734933701316 28.584324432739717 30.086046612842893 23.491894020716074 4 11.839232547929612 17.88361761006672 40.260144216831996 30.017005625171674 5 12.056762412022067 38.49179379874453 35.54737683089214 13.90406695834126 6 27.796819509097283 39.148706999889946 19.567914642980604 13.486558848032168 7 26.34238163998389 32.97786740536359 23.985958547850444 16.69379240680208 8 25.07549899504639 35.42552422352969 21.568092932143372 17.93088384928055 9 26.492016198946043 16.616506633483016 22.191834654834842 34.6996425127361 10-11 23.567581208430425 27.285113304098296 29.37501793165674 19.77228755581454 12-13 24.3725045442836 25.5434438078452 29.257286930461525 20.826764717409677 14-15 22.28330372184013 26.772779992224176 27.155414717796138 23.78850156813955 16-17 20.637693719889928 29.5499901711678 28.297006228275634 21.51530988066664 18-19 20.313581849771424 28.623162210970836 30.69007793694269 20.373178002315047 20-21 21.22589660628783 27.992169703113927 30.625472363725205 20.156461326873035 22-23 21.440548507255656 27.602266480155286 30.264787761609956 20.692397250979106 24-25 20.90103268592507 27.96184960986431 30.662041223547497 20.475076480663127 26-27 20.67254420158925 28.083275743153095 30.517766611833252 20.726413443424395 28-29 20.523819838953706 27.614833052653402 31.043791396991434 20.81755571140146 30-31 21.036359628719442 27.83473917321636 30.672439104289307 20.45646209377489 32-33 20.852576413112 28.233136128630647 30.348769428499082 20.565518029758266 34-35 21.066445727875703 28.04944393383892 30.51884743259119 20.365262905694188 36-37 21.19230670000409 27.80021505999921 30.07064376214507 20.93683447785163 38-39 20.810304497417683 28.314588321360546 30.33920792220877 20.535899259013 40-41 20.793418627128457 27.726707183832673 30.932714336016225 20.54715985302264 42-43 20.768891518837364 27.764025014686894 30.22856143324005 21.238522033235697 44-45 20.545181650082256 28.269949630196184 30.203141665415767 20.98172705430579 46-47 20.75724806912011 27.993183535743583 29.873915050419363 21.375653344716945 48-49 20.804714190695023 28.141270131974988 29.77486959536083 21.279146081969152 50-51 20.915616683846057 28.004578228039723 29.575210038427947 21.504595049686273 52-53 20.804930530440494 28.416624158725273 29.432331150730384 21.34611416010385 54-55 20.962826004290957 28.324092174922765 29.362299179103818 21.350782641682457 56-57 21.01783902163816 28.427681036795132 29.051122737011514 21.50335720455519 58-59 20.695118858384692 28.108655430792574 29.656467772189643 21.53975793863309 60-61 21.088266065448675 28.819136986703818 28.9895044107231 21.103092537124404 62-63 20.935184016657914 28.47541646692462 29.448297878073447 21.14110163834402 64-65 20.800603561588805 28.00679219909434 29.40586221067067 21.786742028646188 66-67 20.910789214256532 28.152128840471075 28.989798627395224 21.94728331787717 68-69 20.585821895285537 28.25673038278386 29.314973084558332 21.842474637372273 70-71 20.969815049681547 27.871446215611922 29.51318495473331 21.645553779973227 72-73 20.995822164337337 28.173014234023498 29.055927887138683 21.775235714500482 74-75 20.867617642131602 28.450166950835875 28.93556464196773 21.746650765064793 76-77 20.814171049933545 28.1110259820545 29.340012674469957 21.734790293542 78-79 20.74457598267615 28.014938531787315 29.095144307307113 22.145341178229426 80-81 20.72583515040148 28.507860048770628 28.77613276389862 21.99017203692927 82-83 21.411413703973963 28.00422493541538 29.04745090818834 21.536910452422322 84-85 20.9475757420062 28.218252676969218 28.734468630887477 22.099702950137107 86-87 20.70728786870941 28.396882313571226 29.22418096945924 21.671648848260126 88-89 21.179983317045707 27.841678185233164 29.116550943071058 21.86178755465007 90-91 21.276760470098157 27.93168077296098 29.168444227229333 21.623114529711525 92-93 21.42857741019937 28.07156567577882 28.88336068042997 21.616496233591835 94-95 20.990300239808445 27.53580929583382 29.23109566171227 22.24279480264547 96-97 21.38688960947705 27.590139625502243 28.97847721217342 22.044493552847285 98-99 21.082355886297236 27.712410766670022 28.972705690239348 22.232527656793398 100 21.463879004697187 27.391088292922657 28.68853507900397 22.456497623376194 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3970.0 1 2660.5 2 3681.5 3 5002.0 4 2996.0 5 2156.0 6 3018.5 7 5257.5 8 8272.0 9 9688.0 10 8319.5 11 6687.0 12 6621.0 13 7185.0 14 7866.0 15 8458.5 16 9046.0 17 9691.0 18 10356.0 19 11140.0 20 12173.5 21 13475.5 22 15045.0 23 17336.0 24 19931.5 25 23382.0 26 30609.0 27 39816.5 28 46203.5 29 57653.0 30 79414.0 31 99118.5 32 125945.0 33 162568.0 34 198774.5 35 228720.5 36 261398.0 37 292921.0 38 297964.0 39 293225.0 40 276405.0 41 254705.0 42 239138.0 43 232487.5 44 236592.5 45 242730.5 46 238956.0 47 225434.5 48 218623.5 49 213654.5 50 205666.0 51 192358.5 52 178398.0 53 167541.0 54 157935.0 55 143878.0 56 128001.0 57 110028.5 58 92820.0 59 80795.0 60 67079.0 61 53220.5 62 42442.0 63 33498.0 64 25057.0 65 17546.0 66 12527.0 67 9474.0 68 7265.5 69 5417.5 70 4281.5 71 3324.5 72 2520.5 73 1945.5 74 1464.5 75 1052.5 76 728.0 77 484.0 78 336.5 79 242.5 80 170.5 81 111.0 82 81.0 83 66.0 84 44.5 85 29.0 86 26.5 87 26.5 88 23.5 89 18.5 90 15.0 91 13.5 92 10.0 93 6.0 94 5.0 95 4.0 96 4.5 97 3.0 98 0.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02534813749767021 2 0.11373188943282402 3 0.01754285866435399 4 0.01704076470431611 5 0.01863833639534574 6 0.04543189589918562 7 0.04669473828352333 8 0.03834172058471124 9 0.03303169658309845 10-11 0.036371382165774686 12-13 0.03663003663003663 14-15 0.032095976021209664 16-17 0.03831889813198225 18-19 0.03682022373611159 20-21 0.04747070167630915 22-23 0.046884925389598285 24-25 0.06559933662737401 26-27 0.05768755301465582 28-29 0.0503311157516765 30-31 0.04885526380853484 32-33 0.0442070909360629 34-35 0.0446255025694278 36-37 0.04078372302671368 38-39 0.045553615647073595 40-41 0.05434786743197958 42-43 0.05620409358727116 44-45 0.05868413345048859 46-47 0.06035777998394821 48-49 0.0590645076626385 50-51 0.05413485787317563 52-53 0.062297688465912764 54-55 0.050407190594106484 56-57 0.055032541013849426 58-59 0.05168524794693019 60-61 0.04783586091997307 62-63 0.05456087699078353 64-65 0.05277311819367894 66-67 0.051107079144462324 68-69 0.057132206664916946 70-71 0.05687355220065501 72-73 0.0590721151468815 74-75 0.05059737770018144 76-77 0.051183153986892306 78-79 0.06022084526757424 80-81 0.06044146231062119 82-83 0.05143420096691125 84-85 0.05648557050426209 86-87 0.05424897013682061 88-89 0.062290080981669764 90-91 0.0682391336596944 92-93 0.0722026329502965 94-95 0.07518476677355182 96-97 0.07138863213629569 98-99 0.08036546354303364 100 0.12356075907477776 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 6572475.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.724434630323444 #Duplication Level Percentage of deduplicated Percentage of total 1 76.04484666377243 38.57331853569494 2 14.653984412683648 14.866301488299008 3 4.599473203411318 6.999170335210851 4 1.8138838700919764 3.6803293518191422 5 0.8900819288708337 2.2574451308320405 6 0.4952876291706071 1.5073910981443401 7 0.30425610623999355 1.0803253280293106 8 0.226062570097957 0.9173516847437384 9 0.14456506898709445 0.6599683252497665 >10 0.681942052280118 6.126620180388595 >50 0.05692569752215265 2.0377043232319156 >100 0.0667236329124835 7.548216034036172 >500 0.014287586477889974 5.052702512856187 >1k 0.007367036537423819 6.452948155219376 >5k 1.785948251496675E-4 0.587823748512661 >10k+ 1.3394611886225063E-4 1.6523837677319877 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 32427 0.49337578309540925 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 25579 0.3891836789033051 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14954 0.227524638739592 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8552 0.13011841049224226 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 1.5214968485996524E-5 0.0 0.0 0.0 6 3.0429936971993048E-5 1.5214968485996524E-5 0.0 0.0 0.0 7 3.0429936971993048E-5 1.5214968485996524E-5 0.0 0.0 0.0 8 3.0429936971993048E-5 1.5214968485996524E-5 0.0 0.0 0.0 9 3.0429936971993048E-5 1.5214968485996524E-5 0.0 0.0 0.0 10-11 3.0429936971993048E-5 1.5214968485996524E-5 0.0 0.0 0.0 12-13 3.0429936971993048E-5 1.5214968485996524E-5 0.0 3.803742121499131E-5 0.0 14-15 3.0429936971993048E-5 1.5214968485996524E-5 0.0 6.0859873943986096E-5 2.2822452728994785E-5 16-17 3.0429936971993048E-5 1.5214968485996524E-5 0.0 9.128981091597914E-5 6.0859873943986096E-5 18-19 3.0429936971993048E-5 1.5214968485996524E-5 0.0 9.128981091597914E-5 6.0859873943986096E-5 20-21 3.0429936971993048E-5 1.5214968485996524E-5 0.0 1.2171974788797218E-4 6.0859873943986096E-5 22-23 3.0429936971993048E-5 1.5214968485996524E-5 0.0 7.83570877028821E-4 6.846735818698435E-5 24-25 3.0429936971993048E-5 1.5214968485996524E-5 0.0 0.001886656092263569 9.128981091597914E-5 26-27 3.0429936971993048E-5 1.5214968485996524E-5 0.0 0.0037428822475551447 9.128981091597914E-5 28-29 3.0429936971993048E-5 1.5214968485996524E-5 0.0 0.008474737446700064 9.128981091597914E-5 30-31 3.0429936971993048E-5 3.0429936971993048E-5 0.0 0.023522341279350625 9.128981091597914E-5 32-33 3.0429936971993048E-5 3.0429936971993048E-5 0.0 0.05729957131826291 9.128981091597914E-5 34-35 3.0429936971993048E-5 3.0429936971993048E-5 0.0 0.10599507795769478 1.0650477940197567E-4 36-37 3.0429936971993048E-5 3.0429936971993048E-5 0.0 0.18735712193656118 1.0650477940197567E-4 38-39 3.0429936971993048E-5 3.0429936971993048E-5 0.0 0.30591215637944613 1.0650477940197567E-4 40-41 3.0429936971993048E-5 3.0429936971993048E-5 0.0 0.4314584688416464 1.0650477940197567E-4 42-43 3.0429936971993048E-5 3.0429936971993048E-5 0.0 0.5565179023122948 1.1411226364497393E-4 44-45 3.0429936971993048E-5 3.0429936971993048E-5 0.0 0.7077927264843153 1.2171974788797219E-4 46-47 3.0429936971993048E-5 3.0429936971993048E-5 0.0 0.893362089623772 1.2171974788797219E-4 48-49 3.0429936971993048E-5 3.0429936971993048E-5 0.0 1.0879234991384523 1.2171974788797219E-4 50-51 3.0429936971993048E-5 3.0429936971993048E-5 0.0 1.2878634000129328 1.2171974788797219E-4 52-53 3.0429936971993048E-5 3.0429936971993048E-5 0.0 1.4825693517282303 1.2932723213097044E-4 54-55 3.0429936971993048E-5 3.0429936971993048E-5 0.0 1.702722338236357 1.369347163739687E-4 56-57 3.0429936971993048E-5 3.0429936971993048E-5 0.0 1.9545224591953563 1.369347163739687E-4 58-59 3.0429936971993048E-5 3.0429936971993048E-5 0.0 2.235778759143245 1.369347163739687E-4 60-61 3.0429936971993048E-5 3.0429936971993048E-5 0.0 2.5179023122948356 1.369347163739687E-4 62-63 3.0429936971993048E-5 3.0429936971993048E-5 0.0 2.80818108855492 1.6736465334596176E-4 64-65 3.0429936971993048E-5 3.0429936971993048E-5 0.0 3.102370111715906 1.6736465334596176E-4 66-67 3.0429936971993048E-5 3.0429936971993048E-5 0.0 3.435167117410108 1.6736465334596176E-4 68-69 3.0429936971993048E-5 3.0429936971993048E-5 0.0 3.7871806283021234 1.6736465334596176E-4 70-71 3.0429936971993048E-5 3.0429936971993048E-5 0.0 4.166489792658017 1.8257962183195828E-4 72-73 3.0429936971993048E-5 3.0429936971993048E-5 0.0 4.533687842099058 2.2822452728994786E-4 74-75 3.0429936971993048E-5 3.0429936971993048E-5 0.0 4.910996238099042 2.2822452728994786E-4 76-77 3.0429936971993048E-5 3.0429936971993048E-5 0.0 5.3141168281355196 2.2822452728994786E-4 78-79 3.0429936971993048E-5 3.0429936971993048E-5 0.0 5.756590934161028 2.2822452728994786E-4 80-81 3.0429936971993048E-5 3.0429936971993048E-5 0.0 6.225531477867927 2.3583201153294612E-4 82-83 3.0429936971993048E-5 3.0429936971993048E-5 0.0 6.6851832833141245 2.4343949577594438E-4 84-85 3.0429936971993048E-5 4.564490545798957E-5 0.0 7.1368776602421455 2.4343949577594438E-4 86-87 3.0429936971993048E-5 4.564490545798957E-5 0.0 7.610618526506377 2.4343949577594438E-4 88 3.0429936971993048E-5 4.564490545798957E-5 0.0 7.978470819592315 2.4343949577594438E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 13165 0.0 52.5627 1 TACCTGG 5280 0.0 51.671387 2 ACCTGGG 6375 0.0 42.6787 3 GTATCAA 24660 0.0 41.605278 1 GTACCTG 6940 0.0 39.856915 1 ATCAACG 29575 0.0 33.890575 3 CAACGCA 29665 0.0 33.803337 5 TCAACGC 30800 0.0 32.679714 4 AACGCAG 31575 0.0 32.026176 6 TATCAAC 31930 0.0 31.511414 2 TATCACG 500 0.0 31.045595 2 ACGCAGA 35360 0.0 28.252953 7 CGCAGAG 35665 0.0 27.86407 8 GTACATG 29645 0.0 27.515884 1 CCTGGGG 5345 0.0 26.901918 4 GCAGAGT 38530 0.0 25.42748 9 TACATGG 31465 0.0 25.369398 2 ACATGGG 30200 0.0 25.284784 3 CATGGGG 15185 0.0 22.930466 4 GAGTACT 22990 0.0 22.38084 12-13 >>END_MODULE