FastQCFastQC Report
Mon 6 Jun 2016
ERR1211343_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211343_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6572475
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT287390.43726297931905406No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT237400.3612033518575575No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145250.2209974172590995No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68720.1045572634357681No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA130750.051.9010281
TACCTGG54500.050.3631482
ACCTGGG61450.043.6017723
GTACCTG69550.040.148491
GTATCAA241600.039.8593141
ATCAACG295350.031.7271443
CAACGCA296700.031.6517435
TCAACGC306200.030.7101844
AACGCAG314900.030.1501126
TATCAAC320350.029.5270022
CCTGGGG48950.028.0869674
ACGCAGA353900.026.5094387
GTACATG298250.026.4034631
CGCAGAG359900.026.028398
TACATGG317650.024.4900232
ACATGGG302400.024.4548113
GCAGAGT389850.023.8121919
TAACGCA5100.023.0016884
GTACTAG21500.022.2644061
CATGGGG153700.021.675764
Produced by FastQC (version 0.11.5)