##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211343_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6572475 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.120209814415425 33.0 31.0 34.0 30.0 34.0 2 32.24498396722696 33.0 31.0 34.0 30.0 34.0 3 32.30639812247289 34.0 31.0 34.0 30.0 34.0 4 35.952092628728145 37.0 35.0 37.0 35.0 37.0 5 35.86618283066881 37.0 35.0 37.0 35.0 37.0 6 35.89000794982103 37.0 35.0 37.0 35.0 37.0 7 35.81512063568138 37.0 35.0 37.0 35.0 37.0 8 35.79860159224645 37.0 35.0 37.0 33.0 37.0 9 37.41634894008726 39.0 37.0 39.0 34.0 39.0 10-11 37.36482649230312 39.0 37.0 39.0 34.0 39.0 12-13 37.31017539054922 39.0 37.0 39.0 34.0 39.0 14-15 38.53491926557347 40.0 38.0 41.0 34.0 41.0 16-17 38.524906142663156 40.0 38.0 41.0 34.0 41.0 18-19 38.567051072845466 40.0 38.0 41.0 34.0 41.0 20-21 38.54886553391226 40.0 38.0 41.0 34.0 41.0 22-23 38.428568005203516 40.0 38.0 41.0 34.0 41.0 24-25 38.347161913890886 40.0 38.0 41.0 34.0 41.0 26-27 38.248561767066434 40.0 38.0 41.0 34.0 41.0 28-29 38.15649241724008 40.0 38.0 41.0 33.5 41.0 30-31 38.137997634072406 40.0 37.5 41.0 34.0 41.0 32-33 38.04762018874169 40.0 37.0 41.0 33.0 41.0 34-35 37.91079167284775 40.0 37.0 41.0 33.0 41.0 36-37 37.75368867892232 40.0 37.0 41.0 33.0 41.0 38-39 37.64606719690832 40.0 37.0 41.0 33.0 41.0 40-41 37.54523387308434 40.0 37.0 41.0 32.5 41.0 42-43 37.438365760234916 40.0 36.5 41.0 32.0 41.0 44-45 37.28231366418282 40.0 36.0 41.0 31.5 41.0 46-47 37.03348563516788 40.0 36.0 41.0 31.0 41.0 48-49 35.46095785834103 39.0 35.0 41.0 24.0 41.0 50-51 36.2234304428697 39.0 35.0 40.5 29.0 41.0 52-53 36.34135184994998 39.0 35.0 40.0 29.5 41.0 54-55 36.19339137843811 39.0 35.0 40.0 29.0 41.0 56-57 36.00784034933568 38.5 35.0 40.0 28.5 41.0 58-59 35.80286520679044 38.0 35.0 40.0 28.0 41.0 60-61 35.57735153043564 38.0 34.0 40.0 28.0 41.0 62-63 35.280876838633844 38.0 34.0 40.0 27.5 41.0 64-65 34.96985709340849 37.0 34.0 40.0 27.0 41.0 66-67 34.64837264500816 37.0 34.0 39.0 26.0 41.0 68-69 34.29727941148502 36.0 33.0 39.0 26.0 40.0 70-71 33.94364565859893 36.0 33.0 39.0 26.0 40.0 72-73 33.55960616054074 35.0 33.0 38.0 26.0 40.0 74-75 33.178147349362305 35.0 32.0 37.0 25.0 39.5 76-77 32.20235672254364 34.0 31.0 36.0 24.5 39.0 78-79 32.62383599785469 35.0 32.0 36.0 25.0 39.0 80-81 32.4714262891833 35.0 32.0 36.0 25.0 38.0 82-83 32.177121404037294 35.0 32.0 35.5 25.0 37.0 84-85 31.857029886001847 34.0 31.0 35.0 24.0 37.0 86-87 31.590060213237784 34.0 31.0 35.0 24.0 36.0 88-89 31.3401140361888 34.0 31.0 35.0 23.0 36.0 90-91 31.106385570123887 34.0 31.0 35.0 22.5 36.0 92-93 30.909468959562417 34.0 30.5 35.0 20.5 35.0 94-95 30.704172705107283 34.0 30.0 35.0 20.0 35.0 96-97 30.516906644757114 34.0 30.0 35.0 20.0 35.0 98-99 30.304108497940277 34.0 29.0 35.0 19.0 35.0 100 27.902312751284715 31.0 25.0 34.0 12.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.3258747028327349 1101 2 -0.33740473022384165 1101 3 -0.3069762645433727 1101 4 -0.18593017162421432 1101 5 -0.24810686679303728 1101 6 -0.2367357761026696 1101 7 -0.24415306925207858 1101 8 -0.25692987195385086 1101 9 -0.38701078570655767 1101 10-11 -0.3345702498136589 1101 12-13 -0.43358392277792746 1101 14-15 -0.4608266752815737 1101 16-17 -0.5373562615244296 1101 18-19 -0.5450351514465908 1101 20-21 -0.5837720918485729 1101 22-23 -0.5968097905253771 1101 24-25 -0.5486105760954345 1101 26-27 -0.5904477698363095 1101 28-29 -0.5874157890055969 1101 30-31 -0.5375995139978187 1101 32-33 -0.5108614710240786 1101 34-35 -0.5272666959602219 1101 36-37 -0.5948521458604077 1101 38-39 -0.5858964818998373 1101 40-41 -0.4498849746572873 1101 42-43 -0.544300320351006 1101 44-45 -0.5300074444383469 1101 46-47 -0.521776106424241 1101 48-49 0.782069452069301 1101 50-51 -0.08339915887601279 1101 52-53 -0.5372968763956081 1101 54-55 -0.5137618687605467 1101 56-57 -0.5130763643897396 1101 58-59 -0.5983709788281644 1101 60-61 -0.5627332094071562 1101 62-63 -0.5905135222538931 1101 64-65 -0.6981997314316573 1101 66-67 -0.6197088025997104 1101 68-69 -0.6341244989382062 1101 70-71 -0.5747840549815137 1101 72-73 -0.6929822153934708 1101 74-75 -0.7190401716006036 1101 76-77 -0.6849978489025048 1101 78-79 -0.5794610835030554 1101 80-81 -0.40596251186286736 1101 82-83 -0.5368405634630378 1101 84-85 -0.5264548986971569 1101 86-87 -0.45649499438796326 1101 88-89 -0.5369212787799142 1101 90-91 -0.5653168884400124 1101 92-93 -0.5757372526748235 1101 94-95 -0.5861074873596408 1101 96-97 -0.7188136376446188 1101 98-99 -0.8425407544041441 1101 100 -0.6781315505521199 1102 1 -0.2736808286055421 1102 2 -0.2831333477236413 1102 3 -0.32215906349119905 1102 4 -0.15272792066812002 1102 5 -0.17150606979264893 1102 6 -0.19017213160365287 1102 7 -0.2020720154854132 1102 8 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1105 68-69 -0.822835642336301 1105 70-71 -0.8581319498825408 1105 72-73 -0.7775119069960041 1105 74-75 -0.8228447275698727 1105 76-77 -0.7894107051861887 1105 78-79 -0.5793857490997709 1105 80-81 -0.5897638783922403 1105 82-83 -0.6416033390229714 1105 84-85 -0.5938741464536683 1105 86-87 -0.5399523382852038 1105 88-89 -0.6395045490336777 1105 90-91 -0.6277630062130619 1105 92-93 -0.5939577188880953 1105 94-95 -0.6835575140467292 1105 96-97 -0.6730979793150418 1105 98-99 -0.6835060392248877 1105 100 -0.7405086155189906 1106 1 -0.3241922987700292 1106 2 -0.27376233593073707 1106 3 -0.3370867765564789 1106 4 -0.24339721603343634 1106 5 -0.25840397724997644 1106 6 -0.3215966753974939 1106 7 -0.31794788085981196 1106 8 -0.3297052559881095 1106 9 -0.4330277713359081 1106 10-11 -0.43008369082632925 1106 12-13 -0.4834789007629894 1106 14-15 -0.6257104624560768 1106 16-17 -0.6153490616935215 1106 18-19 -0.5518787291487754 1106 20-21 -0.6222265699947798 1106 22-23 -0.6326323786500581 1106 24-25 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0.9355276735815359 2202 90-91 0.9657269562934125 2202 92-93 1.0008353700041965 2202 94-95 0.9049167598964765 2202 96-97 0.9005746092061564 2202 98-99 0.23860496596572744 2202 100 0.7405313065847601 2203 1 0.5701277020781745 2203 2 0.5467696515592309 2203 3 0.5130993208461234 2203 4 0.3273709336917534 2203 5 0.36418030719283934 2203 6 0.4122504720197284 2203 7 0.4459384923595948 2203 8 0.5184240238297946 2203 9 0.5877620552850473 2203 10-11 0.5459992655643688 2203 12-13 0.5983514797705212 2203 14-15 0.7810426974131488 2203 16-17 0.7837252107731203 2203 18-19 0.7534566184289773 2203 20-21 0.7295439389642837 2203 22-23 0.7341040199279902 2203 24-25 0.7337510508728329 2203 26-27 0.767118596954937 2203 28-29 0.6686797480356788 2203 30-31 0.787636159721913 2203 32-33 0.8094496426667419 2203 34-35 0.893330427874254 2203 36-37 0.8763944907976793 2203 38-39 0.9007261887913529 2203 40-41 0.8838169458432716 2203 42-43 0.975422189167098 2203 44-45 0.9559682443281687 2203 46-47 0.9759613441845545 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1.0814362632216756 2204 74-75 1.09785785577958 2204 76-77 1.1596521528814705 2204 78-79 0.9667267619393769 2204 80-81 0.9303432906467606 2204 82-83 0.89115422836052 2204 84-85 0.9052559847082158 2204 86-87 0.9144451790321853 2204 88-89 0.7253404439633009 2204 90-91 0.11177130313306805 2204 92-93 0.6139341030899494 2204 94-95 0.837883748118692 2204 96-97 0.936301480675759 2204 98-99 0.8985137751816126 2204 100 1.1412723664684918 2205 1 0.4830985471306093 2205 2 0.47121210154351445 2205 3 0.4584014967385528 2205 4 0.3095879527230281 2205 5 0.07039126428776399 2205 6 0.31427024393881453 2205 7 0.37822720601766235 2205 8 0.41447921168644797 2205 9 0.5829611451768173 2205 10-11 0.5817510666653547 2205 12-13 0.6130745916834641 2205 14-15 0.7914473208848705 2205 16-17 0.7613958425909644 2205 18-19 0.7279267612179083 2205 20-21 0.7451951408413038 2205 22-23 0.7086906583541932 2205 24-25 0.773212182348793 2205 26-27 0.7522988297194573 2205 28-29 0.7639329597040572 2205 30-31 0.792558741605724 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0.9908468917613504 2206 58-59 0.9621100606548865 2206 60-61 0.9488093227874685 2206 62-63 0.9217020058556145 2206 64-65 0.9585827590089977 2206 66-67 0.9802749696964455 2206 68-69 1.0127877598879849 2206 70-71 0.9659426412007761 2206 72-73 1.0256430621172044 2206 74-75 1.047572205067027 2206 76-77 1.023387045610157 2206 78-79 0.92235962614496 2206 80-81 0.95253423400451 2206 82-83 0.8659908501768996 2206 84-85 0.8913719540020075 2206 86-87 0.9318157827193829 2206 88-89 0.97097920485648 2206 90-91 0.9174538401221781 2206 92-93 0.9177240959841093 2206 94-95 0.9755590820940832 2206 96-97 1.0064133263371424 2206 98-99 1.043176095034955 2206 100 1.2583727237113251 2207 1 0.5468329687544511 2207 2 0.5153446779794706 2207 3 0.498353416813643 2207 4 0.32683449312421686 2207 5 0.35469375464389685 2207 6 0.38558023432128863 2207 7 0.40866977412083116 2207 8 0.4299869916565484 2207 9 0.5752433562469719 2207 10-11 0.5644986164828794 2207 12-13 0.5757094856529932 2207 14-15 0.7602998828906848 2207 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1.2373952555689272 2311 1 0.3880985321498187 2311 2 0.4401258603094007 2311 3 0.4244502605471894 2311 4 0.27908561938369303 2311 5 0.3080218151083258 2311 6 0.3657064812003199 2311 7 0.38408299847386473 2311 8 0.17962900533237303 2311 9 0.39658554600386253 2311 10-11 0.3753314586227532 2311 12-13 0.47479402616909283 2311 14-15 0.46850505789821284 2311 16-17 0.4568055713617696 2311 18-19 0.6284652592684026 2311 20-21 0.6561103090288327 2311 22-23 0.6665947905363154 2311 24-25 0.6669177342983303 2311 26-27 0.6910994217891826 2311 28-29 0.7495471953969428 2311 30-31 0.5916471099676954 2311 32-33 0.6284601517858306 2311 34-35 0.7516644335539766 2311 36-37 0.7650013519691683 2311 38-39 0.7754595095333343 2311 40-41 0.6918544858632245 2311 42-43 0.7251686678122837 2311 44-45 0.7737302314976802 2311 46-47 0.7769459260935037 2311 48-49 -6.190429974097924 2311 50-51 -1.113409525484549 2311 52-53 0.2556719732281394 2311 54-55 0.7108066064663063 2311 56-57 0.8464309348184784 2311 58-59 0.8721280550967947 2311 60-61 0.7271735161149167 2311 62-63 0.5853218211868025 2311 64-65 0.7007526490651514 2311 66-67 0.8251849462129925 2311 68-69 0.921191166058442 2311 70-71 1.0038100389673588 2311 72-73 0.361835719226292 2311 74-75 0.7410313189855913 2311 76-77 0.7653449135728394 2311 78-79 0.7179228591931306 2311 80-81 0.7390062889168831 2311 82-83 0.7711700003002377 2311 84-85 0.8178761009814473 2311 86-87 0.7751866155677121 2311 88-89 0.7589773690707879 2311 90-91 0.8290889865307633 2311 92-93 0.768379862238433 2311 94-95 0.7490774146739021 2311 96-97 0.7892919171071533 2311 98-99 0.8248917036930834 2311 100 1.015120702395052 2312 1 0.0878128283188957 2312 2 0.23577840266028005 2312 3 0.22308396517851037 2312 4 0.061761312588778594 2312 5 0.2175083785506473 2312 6 0.13221115550530982 2312 7 0.26713277405324476 2312 8 0.3392226503699618 2312 9 0.23833066538566072 2312 10-11 0.2231606439822471 2312 12-13 0.26716803144819323 2312 14-15 0.26444407574314965 2312 16-17 0.4442110244028825 2312 18-19 0.3058747836326745 2312 20-21 0.3424884170665763 2312 22-23 0.6151366121356929 2312 24-25 0.669527440348368 2312 26-27 0.6179842596718217 2312 28-29 0.7172451365036849 2312 30-31 0.7270364739391084 2312 32-33 0.7189412193864015 2312 34-35 0.33528994984373384 2312 36-37 0.6764684404119734 2312 38-39 0.7901308971426033 2312 40-41 0.5621734554873967 2312 42-43 0.6233978573821446 2312 44-45 0.7552991641833771 2312 46-47 0.7424996838280435 2312 48-49 -4.926025521480721 2312 50-51 -0.8189436285678582 2312 52-53 0.5047499424625386 2312 54-55 0.7942613522817155 2312 56-57 0.8697047750576772 2312 58-59 0.917746797179916 2312 60-61 0.9620910223030705 2312 62-63 0.7251058778369739 2312 64-65 0.9247807971315893 2312 66-67 0.8566772122103288 2312 68-69 0.8166921086076258 2312 70-71 0.8552297071102473 2312 72-73 0.9825778945176467 2312 74-75 0.656332230803379 2312 76-77 0.8387912240789248 2312 78-79 0.5465166873318097 2312 80-81 0.7135406942290032 2312 82-83 0.7397090364446228 2312 84-85 0.741006353202561 2312 86-87 0.8573986547999226 2312 88-89 0.8702816774865489 2312 90-91 0.6645078194994412 2312 92-93 0.8160544020382723 2312 94-95 0.9418185609151273 2312 96-97 0.9757115776045318 2312 98-99 0.9677395563661584 2312 100 0.5383005917584711 2313 1 0.021952278313143836 2313 2 0.16847072437686705 2313 3 0.22910100882473472 2313 4 0.05649919137156445 2313 5 0.11452247913060631 2313 6 0.2183956934633784 2313 7 0.09988429153840173 2313 8 0.18649571886972893 2313 9 0.38365892291587755 2313 10-11 0.24093443467242537 2313 12-13 0.026446328794449414 2313 14-15 0.07660031960272562 2313 16-17 0.0763431825855605 2313 18-19 0.37464401432884387 2313 20-21 0.35291690563056477 2313 22-23 0.40938055629697345 2313 24-25 0.23880954614116234 2313 26-27 0.33399984411453687 2313 28-29 0.3198403123063329 2313 30-31 0.4863813376937003 2313 32-33 0.340405640005784 2313 34-35 0.2747791428000568 2313 36-37 -0.5051387810104941 2313 38-39 -0.03050133652722309 2313 40-41 0.22825293011442938 2313 42-43 0.284604043414582 2313 44-45 0.35753212544694435 2313 46-47 0.2941931892225682 2313 48-49 -5.516918568613434 2313 50-51 -1.4872421050162572 2313 52-53 -0.09317523940452332 2313 54-55 0.12344837003776377 2313 56-57 0.09238318210538665 2313 58-59 0.28230148045830106 2313 60-61 0.5840548521010547 2313 62-63 0.6752575763217052 2313 64-65 0.6946004576228972 2313 66-67 0.7396661182272766 2313 68-69 0.7867233264723765 2313 70-71 0.7763021202709339 2313 72-73 0.48311019682974177 2313 74-75 0.634067547975107 2313 76-77 0.6499926427693481 2313 78-79 0.5940384547169231 2313 80-81 -0.3442941008009015 2313 82-83 0.07709948636806985 2313 84-85 0.4567751416264336 2313 86-87 0.6057581362664166 2313 88-89 0.5947698513408461 2313 90-91 0.6654113418952825 2313 92-93 0.6794540266953142 2313 94-95 0.6477514374858764 2313 96-97 0.6072018066564091 2313 98-99 0.770416260331384 2313 100 0.8329594354390757 2314 1 0.08706874792004271 2314 2 0.2082431982506705 2314 3 0.18794431999962313 2314 4 0.07318719328186063 2314 5 0.22427348715559248 2314 6 0.2543250016335463 2314 7 0.056154918413774624 2314 8 0.18087459277170836 2314 9 0.38117569451916467 2314 10-11 0.2739657024296136 2314 12-13 0.1366513385001582 2314 14-15 0.1455911898785942 2314 16-17 0.4434474706857259 2314 18-19 0.4907257059912169 2314 20-21 0.49410670903289855 2314 22-23 0.2887607858223973 2314 24-25 0.3969074638977119 2314 26-27 0.48135463663668787 2314 28-29 0.6043184838821674 2314 30-31 0.4967280253748285 2314 32-33 0.46153051360229114 2314 34-35 0.3561970694580836 2314 36-37 0.487656499610047 2314 38-39 0.44527259082660464 2314 40-41 0.29178643081485234 2314 42-43 0.20065790092793634 2314 44-45 0.4285231281876136 2314 46-47 0.526394626984569 2314 48-49 -6.58115818062781 2314 50-51 -1.6060778254134291 2314 52-53 -0.7626114090338021 2314 54-55 -0.43122014962241195 2314 56-57 -0.18083978757564978 2314 58-59 0.27309682322442086 2314 60-61 0.5215585436166279 2314 62-63 0.4098784670384745 2314 64-65 0.5934414571508668 2314 66-67 0.6085360089629503 2314 68-69 0.6074971359247456 2314 70-71 0.42643903029215124 2314 72-73 0.5609076001629631 2314 74-75 0.5630265790204234 2314 76-77 0.5046521081817801 2314 78-79 0.3254873341552482 2314 80-81 0.35594551808971175 2314 82-83 0.36814875026568217 2314 84-85 0.42517987139808255 2314 86-87 0.485604198161667 2314 88-89 0.38744301719400553 2314 90-91 0.5964737299922866 2314 92-93 0.6739329141129851 2314 94-95 0.6860687041649811 2314 96-97 0.6470894168938592 2314 98-99 0.7709850902426645 2314 100 -0.6186204488929512 2315 1 0.10932680378494553 2315 2 0.19116599104250298 2315 3 0.28099519998229283 2315 4 0.10137051525842367 2315 5 0.22785083744084744 2315 6 0.28353967636946464 2315 7 0.32517525306574413 2315 8 0.29458855759011016 2315 9 0.4104805898385493 2315 10-11 0.18474955526252046 2315 12-13 0.4206265648132188 2315 14-15 0.2567505647345669 2315 16-17 0.35419296675182466 2315 18-19 0.4357324360822119 2315 20-21 0.3697014748850975 2315 22-23 0.4118817761814242 2315 24-25 0.4280059489730448 2315 26-27 0.4990722244689678 2315 28-29 0.5334755046709176 2315 30-31 0.5225431058560162 2315 32-33 0.5862262144157313 2315 34-35 0.6101040568535083 2315 36-37 0.44805692147264153 2315 38-39 0.6082361072109421 2315 40-41 0.612616244720904 2315 42-43 0.6516384899628918 2315 44-45 0.6369519113397715 2315 46-47 0.5518709083446822 2315 48-49 -4.876966786838267 2315 50-51 -0.920151429266177 2315 52-53 0.16381375396862552 2315 54-55 0.1558596541940389 2315 56-57 0.4994101488273017 2315 58-59 0.6200089456421622 2315 60-61 0.4909923176725144 2315 62-63 0.22727021600810104 2315 64-65 0.46322571799432666 2315 66-67 -0.30243367158327317 2315 68-69 0.4977786540885063 2315 70-71 0.4468789233345376 2315 72-73 0.5758156728091421 2315 74-75 0.6972621463959285 2315 76-77 0.7088261869098531 2315 78-79 0.5887350323586702 2315 80-81 0.46055853037142214 2315 82-83 0.514389448627675 2315 84-85 0.5538792896120235 2315 86-87 0.5001115345401956 2315 88-89 0.4348354145020181 2315 90-91 0.545934173799445 2315 92-93 0.5823555556258562 2315 94-95 0.21031029470830376 2315 96-97 0.2950580086742285 2315 98-99 0.24336144833583973 2315 100 0.3143449090067669 2316 1 0.22600834269630354 2316 2 0.2699093491820648 2316 3 0.25933309060704346 2316 4 -0.018251791899459135 2316 5 0.20817266771356913 2316 6 0.21498452405319313 2316 7 0.26441841381629416 2316 8 0.32534707487126013 2316 9 0.45489948469420227 2316 10-11 0.4176531681953364 2316 12-13 0.3987085781142383 2316 14-15 0.5623911116918663 2316 16-17 0.48605079578914 2316 18-19 0.16826039727433795 2316 20-21 0.5332242332785029 2316 22-23 0.633475590502492 2316 24-25 0.647606200208287 2316 26-27 0.6398965731399002 2316 28-29 0.7008764200825581 2316 30-31 0.6872829276514949 2316 32-33 0.6194307169561952 2316 34-35 0.608214663261613 2316 36-37 0.4889719362739271 2316 38-39 0.6012955342952893 2316 40-41 0.1630276310117793 2316 42-43 0.1537246374349337 2316 44-45 0.447928956071884 2316 46-47 0.5335304045184017 2316 48-49 -5.043726533668369 2316 50-51 -0.9510121945695502 2316 52-53 0.35622239868114036 2316 54-55 0.6368550045219052 2316 56-57 0.5157244004296686 2316 58-59 0.379109059817786 2316 60-61 0.5063150175116888 2316 62-63 0.4205179328494779 2316 64-65 0.334116309040958 2316 66-67 0.41892092352870947 2316 68-69 0.18241795252332338 2316 70-71 -0.22423095506893986 2316 72-73 -0.11443170212282894 2316 74-75 0.07485621361161066 2316 76-77 0.18197659431330493 2316 78-79 0.298249406189143 2316 80-81 0.16371184394056115 2316 82-83 0.09888858735813244 2316 84-85 0.24785066164508507 2316 86-87 0.27895135377585234 2316 88-89 0.15044140841325415 2316 90-91 0.20274638918847998 2316 92-93 0.2545524403268793 2316 94-95 0.4410783325200356 2316 96-97 0.5247685215139057 2316 98-99 0.5127604660087464 2316 100 -0.20599070955405097 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 5.0 11 16.0 12 33.0 13 192.0 14 579.0 15 1903.0 16 4707.0 17 10296.0 18 17362.0 19 24541.0 20 33410.0 21 45744.0 22 56235.0 23 55358.0 24 52811.0 25 55389.0 26 64924.0 27 79417.0 28 97626.0 29 120915.0 30 149680.0 31 186291.0 32 234193.0 33 305618.0 34 423782.0 35 600523.0 36 928585.0 37 1475850.0 38 1357077.0 39 189392.0 40 21.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.545813356840675 21.362358116233164 14.506080346936145 22.58574817999002 2 15.057195536584542 21.606617769004345 41.109716044419926 22.22647064999119 3 17.515637195757456 28.43319178330217 30.62454450153443 23.426626519405946 4 11.83120883402121 18.27498569463214 40.131000645263406 29.76280482608325 5 11.920130885683017 38.69448743110069 35.584339667396634 13.801042015819664 6 27.835827980704973 39.45343548059238 19.263239158721408 13.447497379981229 7 26.29035885117233 33.11355815177055 23.776024066432214 16.820058930624906 8 25.103355848888885 35.88624930141321 21.248130571862042 17.76226427783586 9 26.24629002229981 16.607416075187867 22.128304172141444 35.01798973037088 10-11 23.559560112117307 27.499923163555728 29.20306000758931 19.737456716737654 12-13 24.31001011111577 25.562499757511237 29.251782758368066 20.875707373004925 14-15 22.15119696692254 26.923279106861937 27.0588979038539 23.86662602236162 16-17 20.575958999745453 29.640185763384626 28.235937087054484 21.547918149815434 18-19 20.229329151448212 28.702165371657205 30.689529491517998 20.378975985376588 20-21 21.122013489601322 28.05234561740107 30.629709987265063 20.19593090573255 22-23 21.29286883558533 27.670921135933707 30.300240655669164 20.735969372811798 24-25 20.846865468056354 28.00754967023445 30.651166665208564 20.494418196500632 26-27 20.61975939030532 28.151227772816 30.516084516171244 20.712928320707434 28-29 20.499389454895926 27.684635404799657 31.052762949577595 20.763212190726822 30-31 20.96522526360635 27.881639608660592 30.708808179784853 20.44432694794821 32-33 20.82649352751991 28.278777703115605 30.35105626687507 20.543672502489414 34-35 20.99849258784037 28.0970182670718 30.55014875147148 20.35434039361635 36-37 21.139950733484863 27.801567936177698 30.149028430932333 20.909452899405107 38-39 20.75850704597878 28.27487466620647 30.419705943628824 20.546912344185934 40-41 20.696958432761413 27.742103788839334 31.011343131516206 20.549594646883047 42-43 20.65707636856738 27.752936339645238 30.36103374736463 21.228953544422748 44-45 20.448261448514195 28.257679424712475 30.26056931358596 21.03348981318737 46-47 20.685079891774873 28.020534806099178 29.907680260646018 21.386705041479935 48-49 20.88717026575585 28.18622031753557 29.716292410218 21.210317006490584 50-51 20.85291879977274 27.982225145368755 29.590094388398164 21.57476166646034 52-53 20.7302488150613 28.384642378758546 29.45207637052812 21.433032435652038 54-55 20.90213743173735 28.31446170710601 29.381404865807376 21.40199599534926 56-57 20.964958369490606 28.337626558072348 29.103635843245485 21.593779229191554 58-59 20.576433977054055 28.133483294502742 29.666443926196035 21.623638802247168 60-61 20.984551904502293 28.8554696896915 28.9835026263147 21.17647577949151 62-63 20.87526827891421 28.487907382624883 29.388373527937144 21.24845081052376 64-65 20.67933321397399 27.98302926249242 29.36458955287194 21.973047970661646 66-67 20.79196001436103 28.18608736334306 28.979109692189052 22.04284293010686 68-69 20.463059500488505 28.274099032205708 29.26227210939951 22.00056935790628 70-71 20.862680664912624 27.901991986276105 29.414761956361605 21.820565392449666 72-73 20.870748883211892 28.248128566209722 28.902773710008628 21.978348840569755 74-75 20.703142980374356 28.52470211708738 28.781038417653637 21.991116484884625 76-77 20.63304868384962 28.21239946694321 29.19299265430096 21.96155919490621 78-79 20.559447103478657 28.11084558809544 28.92223523208918 22.407472076336727 80-81 20.500160750251016 28.67455065626119 28.567137840639518 22.258150752848277 82-83 21.154971488831205 28.17505313626661 28.79707557466487 21.87289980023731 84-85 20.728661378928937 28.353157530612883 28.513188247744687 22.404992842713494 86-87 20.427271486273447 28.55763952584641 28.983026962155094 22.032062025725043 88-89 20.84960316587585 28.0463134603374 28.849570290737226 22.254513083049527 90-91 20.91994051851583 28.063227572831757 28.909163969828285 22.107667938824132 92-93 21.003068585561692 28.259100588144932 28.621919687832232 22.115911138461144 94-95 20.49833940627631 27.720701023592802 29.003718323986423 22.777241246144467 96-97 20.862933835972562 27.843131765385387 28.790494933083714 22.50343946555833 98-99 20.474356124017774 27.99009132434683 28.733112650201715 22.80243990143368 100 20.566349775337027 27.665644996275656 28.761883424861566 23.006121803525755 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3865.0 1 2638.5 2 3266.0 3 4387.5 4 2749.5 5 1954.5 6 2790.5 7 4926.0 8 7472.5 9 8780.5 10 7633.0 11 6037.0 12 5992.5 13 6470.0 14 7289.5 15 8164.0 16 8765.5 17 9512.5 18 10389.5 19 11094.5 20 12046.5 21 13576.0 22 15347.0 23 17681.5 24 20171.5 25 23685.5 26 30969.5 27 39835.5 28 46084.0 29 57693.0 30 79657.5 31 100097.0 32 127228.5 33 163962.5 34 199634.5 35 229560.5 36 263175.0 37 294355.0 38 298972.5 39 292873.5 40 275614.5 41 253845.0 42 238331.0 43 232759.5 44 237664.5 45 245587.0 46 241650.0 47 227607.5 48 221251.5 49 215102.0 50 206015.5 51 192758.5 52 180042.5 53 169056.5 54 158302.5 55 144621.5 56 127998.0 57 109663.0 58 92846.5 59 80173.5 60 66030.0 61 51539.5 62 40513.0 63 31624.0 64 23175.0 65 15857.0 66 11156.5 67 8429.0 68 6470.5 69 4728.5 70 3568.5 71 2742.0 72 2081.0 73 1572.0 74 1153.5 75 813.5 76 531.0 77 294.5 78 158.5 79 102.0 80 68.0 81 43.5 82 29.5 83 21.0 84 10.5 85 6.0 86 2.5 87 1.5 88 2.0 89 2.0 90 1.5 91 0.5 92 0.0 93 0.0 94 0.5 95 1.0 96 0.5 97 0.0 98 0.0 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.031099395585376895 2 0.001658431564973621 3 7.607484242998262E-5 4 0.02305067725628473 5 3.347293066919235E-4 6 4.564490545798957E-5 7 1.5214968485996522E-5 8 0.004229761239107033 9 0.01019402888561767 10-11 0.0011106926994777463 12-13 8.368232667298088E-5 14-15 1.2932723213097044E-4 16-17 4.260191176079027E-4 18-19 7.607484242998261E-6 20-21 7.607484242998262E-5 22-23 2.1300955880395136E-4 24-25 3.803742121499131E-5 26-27 7.607484242998262E-5 28-29 0.0010574403097767583 30-31 0.0026626194850493915 32-33 0.009904944484383737 34-35 0.019467552177832553 36-37 0.02252576084351785 38-39 0.021635685187087056 40-41 0.004625350419742943 42-43 0.005279594064640794 44-45 0.009068121217653928 46-47 0.012225227178498208 48-49 1.2182397042210127 50-51 0.017793905644372932 52-53 0.019003495639009656 54-55 0.009372420587373859 56-57 0.007485764495110289 58-59 0.008391055120027082 60-61 0.005142659348266825 62-63 0.007455334558138297 64-65 0.019497982114804545 66-67 0.02915187961916934 68-69 0.02914427213492634 70-71 0.026580549945035926 72-73 0.02298220989809775 74-75 0.009729972346794777 76-77 0.014340107798051723 78-79 0.008216082982438122 80-81 0.002556114705647416 82-83 0.014111883270761776 84-85 0.023567986184808615 86-87 0.0231876119726587 88-89 0.032871939413995487 90-91 0.02060867481428229 92-93 0.013396779751919939 94-95 0.006512006512006512 96-97 0.00354508765723719 98-99 0.016158296532128306 100 0.03330556601584639 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 6572475.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.99167661265949 #Duplication Level Percentage of deduplicated Percentage of total 1 75.84193176391099 38.673072581847364 2 14.902228845795495 15.197792682252992 3 4.5740670647170925 6.997180457060118 4 1.8244686903150966 3.7213086978587917 5 0.8774382184760393 2.2371022942059127 6 0.49346465005378204 1.509755391319298 7 0.306172914551714 1.0928589172463499 8 0.2085480587702685 0.8507372136809148 9 0.14024542165074816 0.6436214268498921 >10 0.687052725482478 6.168644650207966 >50 0.05510125119519535 1.970800421526693 >100 0.0682360188883262 7.75708090464811 >500 0.01350941962938555 4.786086287525354 >1k 0.007222857771003118 6.3198517519748725 >5k 1.7834216718526133E-4 0.550776057785966 >10k+ 1.33756625388946E-4 1.5233302640093644 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 28739 0.43726297931905406 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 23740 0.3612033518575575 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14525 0.2209974172590995 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6872 0.1045572634357681 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 1.5214968485996524E-5 0.0 0.0 0.0 0.0 7 1.5214968485996524E-5 0.0 0.0 0.0 0.0 8 1.5214968485996524E-5 0.0 0.0 0.0 0.0 9 1.5214968485996524E-5 0.0 0.0 0.0 0.0 10-11 1.5214968485996524E-5 0.0 0.0 0.0 0.0 12-13 1.5214968485996524E-5 0.0 0.0 4.564490545798957E-5 7.607484242998262E-6 14-15 1.5214968485996524E-5 0.0 0.0 7.607484242998262E-5 1.5214968485996524E-5 16-17 1.5214968485996524E-5 0.0 0.0 1.0650477940197567E-4 1.5214968485996524E-5 18-19 1.5214968485996524E-5 0.0 0.0 1.2171974788797219E-4 1.5214968485996524E-5 20-21 1.5214968485996524E-5 0.0 0.0 1.5214968485996523E-4 1.5214968485996524E-5 22-23 1.5214968485996524E-5 0.0 0.0 8.140008140008141E-4 3.0429936971993048E-5 24-25 1.5214968485996524E-5 0.0 0.0 0.0019323009977215587 6.0859873943986096E-5 26-27 1.5214968485996524E-5 0.0 0.0 0.0038037421214991308 6.0859873943986096E-5 28-29 1.5214968485996524E-5 0.0 0.0 0.008512774867915055 6.0859873943986096E-5 30-31 1.5214968485996524E-5 0.0 0.0 0.02354516373207962 6.0859873943986096E-5 32-33 1.5214968485996524E-5 0.0 0.0 0.05736803867644989 6.0859873943986096E-5 34-35 1.5214968485996524E-5 0.0 0.0 0.10621569500074174 7.607484242998262E-5 36-37 1.5214968485996524E-5 0.0 0.0 0.18672570074439232 7.607484242998262E-5 38-39 1.5214968485996524E-5 0.0 0.0 0.3000163560911224 7.607484242998262E-5 40-41 1.5214968485996524E-5 0.0 0.0 0.4197961954971301 7.607484242998262E-5 42-43 1.5214968485996524E-5 0.0 0.0 0.5389598286794548 7.607484242998262E-5 44-45 1.5214968485996524E-5 0.0 0.0 0.6835248517491508 9.128981091597914E-5 46-47 1.5214968485996524E-5 0.0 0.0 0.8606194774419074 1.0650477940197567E-4 48-49 1.5214968485996524E-5 0.0 0.0 1.0482352538427304 1.0650477940197567E-4 50-51 1.5214968485996524E-5 0.0 0.0 1.2482284071069119 1.0650477940197567E-4 52-53 1.5214968485996524E-5 0.0 0.0 1.4426757043579475 1.2932723213097044E-4 54-55 1.5214968485996524E-5 0.0 0.0 1.6619766526308584 1.5214968485996523E-4 56-57 1.5214968485996524E-5 0.0 0.0 1.9135561565468109 1.5214968485996523E-4 58-59 1.5214968485996524E-5 0.0 0.0 2.1945385870619516 1.5214968485996523E-4 60-61 1.5214968485996524E-5 0.0 0.0 2.4767686449929442 1.5214968485996523E-4 62-63 1.5214968485996524E-5 0.0 0.0 2.7670017763475707 1.6736465334596176E-4 64-65 1.5214968485996524E-5 0.0 0.0 3.0606963130327616 1.6736465334596176E-4 66-67 1.5214968485996524E-5 0.0 0.0 3.3928847199875234 1.8257962183195828E-4 68-69 1.5214968485996524E-5 0.0 0.0 3.744814548552866 1.977945903179548E-4 70-71 1.5214968485996524E-5 0.0 0.0 4.123446646811132 2.0540207456095307E-4 72-73 1.5214968485996524E-5 0.0 0.0 4.491040285432809 2.586544642619409E-4 74-75 1.5214968485996524E-5 0.0 0.0 4.868462793696438 2.586544642619409E-4 76-77 1.5214968485996524E-5 0.0 0.0 5.271377981658356 2.586544642619409E-4 78-79 1.5214968485996524E-5 0.0 0.0 5.714034667305695 2.586544642619409E-4 80-81 1.5214968485996524E-5 0.0 0.0 6.182883921201678 2.6626194850493915E-4 82-83 1.5214968485996524E-5 0.0 0.0 6.642954138281241 2.738694327479374E-4 84-85 1.5214968485996524E-5 0.0 0.0 7.095241898980217 2.738694327479374E-4 86-87 1.5214968485996524E-5 0.0 0.0 7.569325102035382 2.8147691699093567E-4 88 1.5214968485996524E-5 0.0 0.0 7.93809029323048 2.8908440123393393E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 13075 0.0 51.901028 1 TACCTGG 5450 0.0 50.363148 2 ACCTGGG 6145 0.0 43.601772 3 GTACCTG 6955 0.0 40.14849 1 GTATCAA 24160 0.0 39.859314 1 ATCAACG 29535 0.0 31.727144 3 CAACGCA 29670 0.0 31.651743 5 TCAACGC 30620 0.0 30.710184 4 AACGCAG 31490 0.0 30.150112 6 TATCAAC 32035 0.0 29.527002 2 CCTGGGG 4895 0.0 28.086967 4 ACGCAGA 35390 0.0 26.509438 7 GTACATG 29825 0.0 26.403463 1 CGCAGAG 35990 0.0 26.02839 8 TACATGG 31765 0.0 24.490023 2 ACATGGG 30240 0.0 24.454811 3 GCAGAGT 38985 0.0 23.812191 9 TAACGCA 510 0.0 23.001688 4 GTACTAG 2150 0.0 22.264406 1 CATGGGG 15370 0.0 21.67576 4 >>END_MODULE