FastQCFastQC Report
Mon 6 Jun 2016
ERR1211342_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211342_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12994641
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT377360.2903966335045347No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT289330.22265332301215557No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161630.1243820433361722No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA204050.051.403331
GTATCAA376500.042.612771
CAACGCA464100.033.583195
ATCAACG471550.033.1824073
TATCAAC486050.032.784262
AACGCAG499750.031.6390676
TCAACGC500050.031.4600984
GTACTAG56150.029.6535381
ACGCAGA560600.027.7538097
CGCAGAG577400.026.8225548
GTGGTAT94650.026.4371131
GTACATG568550.026.0097921
TACATGG586150.025.0270422
ACATGGG591350.024.0286063
GCAGAGT629400.023.9284449
GCATCGT61050.022.0895028
CATGGGG401050.021.6165264
GAGTACT316050.021.499212-13
TAGGCAT81350.021.3136025
TGGTATC112300.021.1083222
Produced by FastQC (version 0.11.5)