##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211342_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 12994641 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.33479370457406 31.0 31.0 34.0 28.0 34.0 2 31.353295562378367 31.0 31.0 34.0 28.0 34.0 3 31.404416559103094 31.0 31.0 34.0 28.0 34.0 4 35.00785154434047 37.0 35.0 37.0 32.0 37.0 5 35.04967601644401 37.0 35.0 37.0 32.0 37.0 6 35.115916014917225 37.0 35.0 37.0 32.0 37.0 7 35.04403038144724 37.0 35.0 37.0 32.0 37.0 8 35.045660130202904 37.0 35.0 37.0 32.0 37.0 9 36.5914351923997 39.0 37.0 39.0 32.0 39.0 10-11 36.523731898403355 39.0 37.0 39.0 32.0 39.0 12-13 36.43720365187465 39.0 37.0 39.0 32.0 39.0 14-15 37.55777954927727 40.0 37.0 41.0 32.0 41.0 16-17 37.557979939576626 40.0 37.0 41.0 32.0 41.0 18-19 37.55741262879059 40.0 37.0 41.0 32.0 41.0 20-21 37.52358172111103 40.0 37.0 41.0 31.5 41.0 22-23 37.399374557558 40.0 37.0 41.0 31.0 41.0 24-25 37.28485750395105 40.0 37.0 41.0 31.0 41.0 26-27 37.13597185947653 40.0 36.0 41.0 30.5 41.0 28-29 37.0064670120552 39.5 36.0 41.0 30.0 41.0 30-31 36.82955308269001 39.0 36.0 41.0 30.0 41.0 32-33 36.716486203812785 39.0 35.5 41.0 30.0 41.0 34-35 36.57855457492054 39.0 35.0 41.0 30.0 41.0 36-37 36.48482278194527 39.0 35.0 41.0 29.5 41.0 38-39 36.33564474770792 39.0 35.0 40.5 29.0 41.0 40-41 36.17117233173275 39.0 35.0 40.0 28.0 41.0 42-43 36.015650682462095 39.0 35.0 40.0 28.0 41.0 44-45 35.87072705586864 38.5 35.0 40.0 27.0 41.0 46-47 35.70183739589266 38.0 34.5 40.0 26.5 41.0 48-49 35.46136111032233 38.0 34.0 40.0 26.0 41.0 50-51 34.78513869679047 37.5 33.5 39.5 25.0 40.5 52-53 35.02701282782648 38.0 33.5 40.0 26.0 40.5 54-55 35.37989483510933 38.0 34.0 40.0 26.0 41.0 56-57 35.267925754932364 38.0 34.0 40.0 26.0 41.0 58-59 35.03367091865023 38.0 34.0 40.0 26.0 41.0 60-61 34.76223752545376 37.0 33.5 40.0 26.0 41.0 62-63 34.48731746417619 37.0 33.0 40.0 25.0 41.0 64-65 34.20475879248992 36.0 33.0 39.5 25.0 41.0 66-67 33.98062520542122 36.0 33.0 39.0 24.5 41.0 68-69 33.64644833204703 35.5 32.5 39.0 24.0 41.0 70-71 33.3164679963071 35.0 32.0 38.5 23.5 40.0 72-73 32.96395356362673 35.0 32.0 37.5 23.0 40.0 74-75 32.604534938672025 35.0 31.5 37.0 22.5 39.5 76-77 32.263824602772786 35.0 31.0 36.5 22.0 39.0 78-79 31.909589191421293 35.0 31.0 36.0 21.0 39.0 80-81 31.58694791953083 35.0 31.0 36.0 20.0 37.5 82-83 31.286580791266182 34.0 30.0 35.0 20.0 37.0 84-85 30.99493006386248 34.0 30.0 35.0 20.0 36.5 86-87 30.751740582906443 34.0 30.0 35.0 19.0 36.0 88-89 30.502524617648152 34.0 29.0 35.0 18.5 36.0 90-91 30.248671740912272 34.0 29.0 35.0 18.0 35.5 92-93 30.017840392820393 34.0 29.0 35.0 17.5 35.0 94-95 29.806144625311312 34.0 29.0 35.0 17.0 35.0 96-97 29.61328073626659 34.0 29.0 35.0 16.0 35.0 98-99 29.376438641129063 34.0 27.5 35.0 15.0 35.0 100 27.663200853336388 32.0 25.0 34.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.31938623355223683 1101 2 -0.2985448157159496 1101 3 -0.3317824623857746 1101 4 -0.23224455830093405 1101 5 -0.2909960925333195 1101 6 -0.19184966484510113 1101 7 -0.3533099457207598 1101 8 -0.4449797955868675 1101 9 -0.38554472107539794 1101 10-11 -0.44605957894999193 1101 12-13 -0.43730324240370066 1101 14-15 -0.5218527836462812 1101 16-17 -0.5391944351575546 1101 18-19 -0.5797856170365208 1101 20-21 -0.602967812028524 1101 22-23 -0.6253648901704096 1101 24-25 -0.5824315911555047 1101 26-27 -0.7018030141996903 1101 28-29 -0.6882155285532221 1101 30-31 -0.6845341983650783 1101 32-33 -0.752823154731125 1101 34-35 -0.7354873980273595 1101 36-37 -0.8232924477743424 1101 38-39 -0.8576562968748078 1101 40-41 -0.860482708084092 1101 42-43 -0.9192511560892953 1101 44-45 -0.9424375806671748 1101 46-47 -0.8888743068961631 1101 48-49 -0.8547023306193395 1101 50-51 -0.8902820236145246 1101 52-53 -0.7323517662142152 1101 54-55 -0.6900634403221915 1101 56-57 -0.6693570705951899 1101 58-59 -0.7481309456893683 1101 60-61 -0.6384714979145301 1101 62-63 -0.7440399000073228 1101 64-65 -0.8045915697710342 1101 66-67 -0.7364134546213776 1101 68-69 -0.741775638126434 1101 70-71 -0.7332547144473622 1101 72-73 -0.8138105247966578 1101 74-75 -0.8507403538243423 1101 76-77 -0.8531655077737454 1101 78-79 -0.846216760697363 1101 80-81 -0.9342195957092265 1101 82-83 -1.1252201358566296 1101 84-85 -1.0173553850681394 1101 86-87 -1.0015206921771629 1101 88-89 -0.9868336297426445 1101 90-91 -1.0072152609137284 1101 92-93 -1.04089101937846 1101 94-95 -1.110306555615388 1101 96-97 -1.1289603674261066 1101 98-99 -1.254310656447288 1101 100 -1.2457689552093818 1102 1 -0.4420885125328624 1102 2 -0.46660017568864376 1102 3 -0.4145173561557982 1102 4 -0.33934395881215096 1102 5 -0.39281719736627707 1102 6 -0.3271729590256385 1102 7 -0.32901315060934877 1102 8 -0.35805155394519517 1102 9 -0.4968909275516822 1102 10-11 -0.4290297154597127 1102 12-13 -0.43791883166944956 1102 14-15 -0.6085136285058823 1102 16-17 -0.6158706777957406 1102 18-19 -0.6236795618981859 1102 20-21 -0.686671359705862 1102 22-23 -0.6959646317620098 1102 24-25 -0.7359123839213666 1102 26-27 -0.6730631227174939 1102 28-29 -0.8071577994286869 1102 30-31 -0.7680773094395832 1102 32-33 -0.7689833105451456 1102 34-35 -0.7968312934364334 1102 36-37 -0.8278845830513077 1102 38-39 -0.9685228615876298 1102 40-41 -0.9187371128264203 1102 42-43 -0.8728252032033552 1102 44-45 -0.98342743714975 1102 46-47 -1.0088412083370457 1102 48-49 -0.9527911315586266 1102 50-51 -0.9381203157194236 1102 52-53 -0.8399197224050567 1102 54-55 -0.7071189770416737 1102 56-57 -0.6917145305757018 1102 58-59 -0.7299227715296865 1102 60-61 -0.828355888536926 1102 62-63 -0.7840057308199277 1102 64-65 -0.8710000384656738 1102 66-67 -0.8179007900243747 1102 68-69 -0.7469676867119901 1102 70-71 -0.8164139295426551 1102 72-73 -0.9404854747915969 1102 74-75 -0.9024672768027351 1102 76-77 -0.9422583072845718 1102 78-79 -0.9009975514996889 1102 80-81 -0.9180939922563596 1102 82-83 -1.0197670774517213 1102 84-85 -1.0550580600536499 1102 86-87 -1.0305036526463418 1102 88-89 -1.1349160019264382 1102 90-91 -1.2043577553497506 1102 92-93 -1.1849886153069207 1102 94-95 -1.2466526180165793 1102 96-97 -1.295689844236417 1102 98-99 -1.210352596334225 1102 100 -1.2654666092547338 1103 1 -0.44746381357117215 1103 2 -0.4775447405604929 1103 3 -0.47493042682384257 1103 4 -0.3837622029576693 1103 5 -0.3487007041430843 1103 6 -0.43674742160867197 1103 7 -0.4112349801029893 1103 8 -0.4352114995950629 1103 9 -0.6427685518237851 1103 10-11 -0.5989463841883023 1103 12-13 -0.6172472728480187 1103 14-15 -0.7531217742909107 1103 16-17 -0.736179749083476 1103 18-19 -0.6673243057488705 1103 20-21 -0.8428295484210082 1103 22-23 -0.7936497083547565 1103 24-25 -0.808116059284913 1103 26-27 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-0.9138569469681848 1103 92-93 -1.0130661264896474 1103 94-95 -1.2639613474267115 1103 96-97 -1.245592248669812 1103 98-99 -1.239119573919293 1103 100 -1.2290950955526796 1104 1 -0.35497525332031543 1104 2 -0.36392061002053566 1104 3 -0.40689078821176494 1104 4 -0.2689729618247867 1104 5 -0.21876178531595514 1104 6 -0.27992319087593387 1104 7 -0.26543938106359377 1104 8 -0.26125833481548 1104 9 -0.36601298368736224 1104 10-11 -0.3167750527929556 1104 12-13 -0.4170716944585209 1104 14-15 -0.5372092292114417 1104 16-17 -0.48459499533008454 1104 18-19 -0.5501588846945467 1104 20-21 -0.5267757796300572 1104 22-23 -0.5295538776729458 1104 24-25 -0.6570285813597607 1104 26-27 -0.6413113101324157 1104 28-29 -0.6390668244758473 1104 30-31 -0.7298766529044016 1104 32-33 -0.6481434064542171 1104 34-35 -0.6391292389142436 1104 36-37 -0.7364915245571879 1104 38-39 -0.691399841632574 1104 40-41 -0.7905093655070914 1104 42-43 -0.7847476987997979 1104 44-45 -0.7144813431493091 1104 46-47 -0.8436514383908289 1104 48-49 -0.7767094535394605 1104 50-51 -0.6797838778894274 1104 52-53 -0.6605958463436394 1104 54-55 -0.6427908974419552 1104 56-57 -0.6025509214575564 1104 58-59 -0.6636651045271478 1104 60-61 -0.5875947378365893 1104 62-63 -0.6107725863748144 1104 64-65 -0.6277450852471915 1104 66-67 -0.6503229826337673 1104 68-69 -0.6011438233024009 1104 70-71 -0.6798921312559401 1104 72-73 -0.6761867686453442 1104 74-75 -0.7455268867041625 1104 76-77 -0.670358982788116 1104 78-79 -0.6966806043515099 1104 80-81 -0.713143768203576 1104 82-83 -0.7494082660558554 1104 84-85 -0.8002630904251795 1104 86-87 -0.8314965098717835 1104 88-89 -0.7862042269483815 1104 90-91 -0.7514915352023053 1104 92-93 -0.7792157981513199 1104 94-95 -0.9340817564275632 1104 96-97 -0.9506668918910428 1104 98-99 -0.952429843601891 1104 100 -1.0634890300790971 1105 1 -0.33836964595876395 1105 2 -0.36381111572450564 1105 3 -0.36860237148140484 1105 4 -0.19399555067479923 1105 5 -0.198710662905917 1105 6 -0.22314433591787974 1105 7 -0.29316137216886773 1105 8 -0.26147290500033193 1105 9 -0.39366804106099806 1105 10-11 -0.40485782965873085 1105 12-13 -0.40110967434712563 1105 14-15 -0.47001404089389354 1105 16-17 -0.5415440308935189 1105 18-19 -0.5393412058293592 1105 20-21 -0.5292428024367908 1105 22-23 -0.5787211630707532 1105 24-25 -0.5850215934535115 1105 26-27 -0.544631895230637 1105 28-29 -0.625817613916773 1105 30-31 -0.6149520316507804 1105 32-33 -0.6267483792502873 1105 34-35 -0.7034062644975805 1105 36-37 -0.7706747272156136 1105 38-39 -0.7084524684999991 1105 40-41 -0.857447864916125 1105 42-43 -0.8253225898875982 1105 44-45 -0.8130336283916435 1105 46-47 -0.736786361812328 1105 48-49 -0.7627411523436578 1105 50-51 -0.7451793975710714 1105 52-53 -0.5987738111138938 1105 54-55 -0.582035013598059 1105 56-57 -0.6090502546171521 1105 58-59 -0.5651513210797816 1105 60-61 -0.5663788102553937 1105 62-63 -0.6386544005169199 1105 64-65 -0.6138984854817764 1105 66-67 -0.6195271056283289 1105 68-69 -0.6042204732759089 1105 70-71 -0.5401256216770136 1105 72-73 -0.5668445171988878 1105 74-75 -0.57682360147043 1105 76-77 -0.641339099976193 1105 78-79 -0.6216829610066164 1105 80-81 -0.6565294937766346 1105 82-83 -0.7417419082461265 1105 84-85 -0.6713179793937911 1105 86-87 -0.6965198619759505 1105 88-89 -0.7835796314098928 1105 90-91 -0.8369081937771234 1105 92-93 -0.7560873989704646 1105 94-95 -0.8039672882380486 1105 96-97 -0.9232337515886719 1105 98-99 -0.9426642080455601 1105 100 -0.9656430118171819 1106 1 -0.4753455811948726 1106 2 -0.5125193473376086 1106 3 -0.47509518967545716 1106 4 -0.37552631283116256 1106 5 -0.38043389032349495 1106 6 -0.3168232297614253 1106 7 -0.3692131394174609 1106 8 -0.33923612353532206 1106 9 -0.49649668049375606 1106 10-11 -0.5292913661764587 1106 12-13 -0.5157341547773768 1106 14-15 -0.606629843749694 1106 16-17 -0.674325146724513 1106 18-19 -0.7366512023653371 1106 20-21 -0.6523421824086597 1106 22-23 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2311 28-29 0.9186544472470004 2311 30-31 0.9925155237390229 2311 32-33 0.9950152978334046 2311 34-35 1.0274982845885674 2311 36-37 1.0028775904244398 2311 38-39 1.0653304142600746 2311 40-41 1.0643225411564217 2311 42-43 1.0785496493750557 2311 44-45 1.074923236223995 2311 46-47 1.1009309036226185 2311 48-49 1.1451600462357518 2311 50-51 1.1625615136796839 2311 52-53 1.0715079034258466 2311 54-55 0.9868332656587882 2311 56-57 0.9401167288417014 2311 58-59 1.0089604792905718 2311 60-61 1.0134870867076486 2311 62-63 0.9540792764500665 2311 64-65 0.9958941052310735 2311 66-67 0.9699150694722078 2311 68-69 1.0323677870139605 2311 70-71 0.9597911226047771 2311 72-73 0.9189478560012603 2311 74-75 0.9627705304208973 2311 76-77 1.0005898089600578 2311 78-79 1.0050468340042151 2311 80-81 1.0087687547908892 2311 82-83 1.0086129776329535 2311 84-85 1.0691337219851071 2311 86-87 1.0742917790588962 2311 88-89 1.11220177262798 2311 90-91 1.1377686512921734 2311 92-93 1.099570046001844 2311 94-95 1.1799846692645382 2311 96-97 1.1747338951349384 2311 98-99 1.2283595823648952 2311 100 1.2680603000289068 2312 1 0.512229238657639 2312 2 0.50566278888396 2312 3 0.5169428694444314 2312 4 0.39316939750317204 2312 5 0.40030834059028564 2312 6 0.44087403715717954 2312 7 0.4783094118438953 2312 8 0.4770422547830364 2312 9 0.6005911657061347 2312 10-11 0.631857404294685 2312 12-13 0.622889879010657 2312 14-15 0.7846700539232145 2312 16-17 0.7486310036691606 2312 18-19 0.7157446719813692 2312 20-21 0.7507074414696859 2312 22-23 0.8034457287400016 2312 24-25 0.8130166477647478 2312 26-27 0.8531750783231828 2312 28-29 0.8347789738498008 2312 30-31 0.8746986015700244 2312 32-33 0.851478950524843 2312 34-35 0.8529421327411839 2312 36-37 0.8592868810649463 2312 38-39 0.964677210852301 2312 40-41 0.9572636388577251 2312 42-43 0.9451217655362854 2312 44-45 0.9416931656912411 2312 46-47 1.0307165945159085 2312 48-49 1.0275589444711741 2312 50-51 0.9693094026979381 2312 52-53 0.7947888598406152 2312 54-55 0.7699720297727453 2312 56-57 0.7627214278816368 2312 58-59 0.758522796974745 2312 60-61 0.7988261151743785 2312 62-63 0.7582118053788491 2312 64-65 0.7577584346265596 2312 66-67 0.8015947497843925 2312 68-69 0.8061247492521417 2312 70-71 0.8279354534701255 2312 72-73 0.8526088904361941 2312 74-75 0.8039523753608293 2312 76-77 0.8491362071812034 2312 78-79 0.8438180209522024 2312 80-81 0.8909641159449926 2312 82-83 0.9038656678073487 2312 84-85 0.9147257588184061 2312 86-87 0.9265983016393058 2312 88-89 0.9720363674942831 2312 90-91 1.0062692606923527 2312 92-93 1.009684039845034 2312 94-95 1.0016779065894816 2312 96-97 1.0054249509785436 2312 98-99 1.1195368915697763 2312 100 1.2415336821253078 2313 1 0.4666189194491821 2313 2 0.4528918343961692 2313 3 0.5028737754719508 2313 4 0.4408467413289756 2313 5 0.44664399960553425 2313 6 0.4427579075223278 2313 7 0.47290703924934263 2313 8 0.48845490704885464 2313 9 0.6002944780345771 2313 10-11 0.6034187449865343 2313 12-13 0.6052474861578219 2313 14-15 0.7683485027048604 2313 16-17 0.7548304679220053 2313 18-19 0.6801469167148042 2313 20-21 0.7358880634369456 2313 22-23 0.7061766563497613 2313 24-25 0.6825533915138422 2313 26-27 0.7636751263844559 2313 28-29 0.8017837160464225 2313 30-31 0.848170064963206 2313 32-33 0.9516244084172811 2313 34-35 0.9126990741943999 2313 36-37 0.8882960820780426 2313 38-39 0.9175515174777757 2313 40-41 0.9223844404109087 2313 42-43 0.8233535414728195 2313 44-45 0.9493094490314817 2313 46-47 0.9750181440838546 2313 48-49 1.0548513991235424 2313 50-51 0.9537799397781228 2313 52-53 0.845229427543984 2313 54-55 0.8108691092796931 2313 56-57 0.8803561014594621 2313 58-59 0.8538043272093603 2313 60-61 0.8788633467907161 2313 62-63 0.8900895139262346 2313 64-65 0.900750418822561 2313 66-67 0.877851762876098 2313 68-69 0.8842806450553056 2313 70-71 0.8880879618604425 2313 72-73 0.9011604279888275 2313 74-75 0.9352245073497798 2313 76-77 0.9649047998655575 2313 78-79 0.9519502239086073 2313 80-81 0.8869054367594309 2313 82-83 0.9499376450027306 2313 84-85 0.9908118551458891 2313 86-87 0.9824202015285799 2313 88-89 0.9942243591588245 2313 90-91 1.0658224495577358 2313 92-93 1.0671550511902126 2313 94-95 1.0854720900874177 2313 96-97 1.2072052288996495 2313 98-99 1.1920317249830603 2313 100 1.2358274670774136 2314 1 0.33291591911821783 2314 2 0.2697822033973978 2314 3 0.3462245688204888 2314 4 0.2558638869323815 2314 5 0.2449208515002823 2314 6 0.2558717659004657 2314 7 0.30094352904810506 2314 8 0.33234723500925156 2314 9 0.41898984713666465 2314 10-11 0.42312146955138275 2314 12-13 0.4488545491400089 2314 14-15 0.5720891570819049 2314 16-17 0.5903381956911886 2314 18-19 0.5860411005546027 2314 20-21 0.6109809715702568 2314 22-23 0.6332727659415198 2314 24-25 0.6698917541059402 2314 26-27 0.6873712148282394 2314 28-29 0.7032928328433456 2314 30-31 0.7589449433789426 2314 32-33 0.7486299784939021 2314 34-35 0.766584853817811 2314 36-37 0.7627577628902813 2314 38-39 0.8218007697060159 2314 40-41 0.8522970841767261 2314 42-43 0.7865581233579562 2314 44-45 0.8673980883318322 2314 46-47 0.6851548611236566 2314 48-49 0.5777199808277942 2314 50-51 0.7916175631884457 2314 52-53 0.7429667731709273 2314 54-55 0.7498130802098686 2314 56-57 0.7083215748317357 2314 58-59 0.7454709055747131 2314 60-61 0.5751146810088343 2314 62-63 0.6912786489184057 2314 64-65 0.6928314843083712 2314 66-67 0.7052223439955654 2314 68-69 0.7511109617718077 2314 70-71 0.7146166028543988 2314 72-73 0.7695116835337998 2314 74-75 0.835156397108058 2314 76-77 0.7979780204259086 2314 78-79 0.7739733607748356 2314 80-81 0.8149813917049151 2314 82-83 0.840274370067231 2314 84-85 0.9246473739552918 2314 86-87 0.9051819925807685 2314 88-89 0.7177649722596229 2314 90-91 0.7309568024129014 2314 92-93 0.9535876683813953 2314 94-95 0.9749569571998471 2314 96-97 1.0418675273481988 2314 98-99 1.0245793916226091 2314 100 1.0428610726374252 2315 1 0.25530627163337627 2315 2 0.29641513277541165 2315 3 0.2912924289569254 2315 4 0.2413514670871848 2315 5 0.25344228755071896 2315 6 0.21554253989572913 2315 7 0.2598622293230761 2315 8 0.2544322955783471 2315 9 0.16574438151955206 2315 10-11 0.30954679694813336 2315 12-13 0.3175074159018365 2315 14-15 0.4350313095530822 2315 16-17 0.30533685660800813 2315 18-19 0.40229887570896494 2315 20-21 0.3157851032080998 2315 22-23 0.32332847404939 2315 24-25 0.49307992010314905 2315 26-27 0.5876585557980647 2315 28-29 0.46789637777430215 2315 30-31 0.49269242427734383 2315 32-33 0.5684970064104604 2315 34-35 0.48299670566351693 2315 36-37 0.6117104641210318 2315 38-39 0.6644286586331702 2315 40-41 0.727734231295031 2315 42-43 0.6882153663023942 2315 44-45 0.6296784233637069 2315 46-47 0.7406120918961818 2315 48-49 0.6284257917744966 2315 50-51 0.5488054271355196 2315 52-53 0.2718376960992188 2315 54-55 0.4573845582049927 2315 56-57 0.4600384713815444 2315 58-59 0.5455907790214098 2315 60-61 0.5886401775329233 2315 62-63 0.5914096612203323 2315 64-65 0.5678695431443472 2315 66-67 0.4965515815843631 2315 68-69 0.5467580596699477 2315 70-71 0.48748042803670444 2315 72-73 0.5831722554269163 2315 74-75 0.29943520356221853 2315 76-77 0.5112547059590398 2315 78-79 0.5642760947175951 2315 80-81 0.6222699548799078 2315 82-83 0.5152988046611853 2315 84-85 0.5488120160042662 2315 86-87 0.5266036061613057 2315 88-89 0.45476072402039946 2315 90-91 0.5736474052129914 2315 92-93 0.7158241655126929 2315 94-95 0.6663749511927328 2315 96-97 0.6568532642537086 2315 98-99 0.8200337661454142 2315 100 0.6678063076798075 2316 1 0.04625145507092299 2316 2 0.044549908954586215 2316 3 0.020330278717892725 2316 4 -0.05822288959938504 2316 5 -0.0812465504598805 2316 6 -0.2362451818366651 2316 7 -0.19987812166476004 2316 8 -0.16943385459727267 2316 9 -0.11083632765880935 2316 10-11 -0.15549930884654373 2316 12-13 -0.11008701507921614 2316 14-15 -0.20977419169515343 2316 16-17 -0.041604890994079824 2316 18-19 -0.026096858199757378 2316 20-21 0.07086313989766779 2316 22-23 -0.042018221038574666 2316 24-25 -0.0261642477903834 2316 26-27 -0.09723662059187888 2316 28-29 -0.07928260823940292 2316 30-31 -0.7439972263614649 2316 32-33 -0.4634671815161866 2316 34-35 -0.5001129826015571 2316 36-37 -0.2919197985770623 2316 38-39 -0.21567152889508634 2316 40-41 0.001182403932688203 2316 42-43 0.0411911669203775 2316 44-45 -0.09622784834773057 2316 46-47 -0.20731826719381274 2316 48-49 -0.07532530973648477 2316 50-51 0.022979265900850976 2316 52-53 -0.28778093943324734 2316 54-55 -0.22882220888915583 2316 56-57 -0.22776950367855164 2316 58-59 -0.6519500880266875 2316 60-61 -0.10814229252940777 2316 62-63 -0.09618173791314177 2316 64-65 -0.40822438390728166 2316 66-67 -0.6716674638463118 2316 68-69 -0.7471576494532712 2316 70-71 -0.461661119268733 2316 72-73 -0.6629476495355604 2316 74-75 -0.48399071571051167 2316 76-77 -0.18910865050559522 2316 78-79 -0.1442415123129166 2316 80-81 -0.18674140864680666 2316 82-83 -0.007291274985341545 2316 84-85 0.07592311327289636 2316 86-87 0.1272301104539686 2316 88-89 0.16818411718426063 2316 90-91 -0.4108471757148493 2316 92-93 -0.4167247515688999 2316 94-95 -0.06449501028841453 2316 96-97 0.11673833879287443 2316 98-99 0.21468820918493492 2316 100 0.06665100488447706 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 366.0 1 956.0 2 567.0 3 587.0 4 535.0 5 455.0 6 560.0 7 1162.0 8 2854.0 9 5189.0 10 9128.0 11 15536.0 12 24087.0 13 31517.0 14 37209.0 15 42853.0 16 50157.0 17 59413.0 18 71051.0 19 84033.0 20 101326.0 21 122920.0 22 137500.0 23 140611.0 24 142461.0 25 149117.0 26 164146.0 27 188478.0 28 220666.0 29 262261.0 30 317501.0 31 390208.0 32 488675.0 33 634387.0 34 870799.0 35 1209518.0 36 1786343.0 37 2506674.0 38 2211017.0 39 511575.0 40 243.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.80782926367419 19.342365559614695 13.468562710622372 23.38124246608874 2 15.63538058968163 19.727227658126257 40.58706222739426 24.050329524797853 3 19.1261716184496 25.50216507492732 28.98902134333857 26.38264196328451 4 12.862737690961515 16.28559221381394 38.02959173722553 32.82207835799901 5 13.770177739600555 37.59339564841241 34.26865988713089 14.367766724856143 6 31.414631907973778 36.685632332270266 17.703595910831986 14.196139848923965 7 28.226819939418824 31.98627742353769 21.808707579760995 17.978195057282495 8 26.449137124553257 34.93407645136198 19.781560498589947 18.83522592549481 9 26.654218917192058 15.951101976089852 20.50255190411307 36.89212720260502 10-11 25.4600974553163 26.00651298239196 27.388960894391484 21.144428667900257 12-13 25.83560670685541 23.566381287946385 27.890191844061906 22.707820161136294 14-15 23.358658167773907 24.994533495364855 25.44310056678829 26.203707770072942 16-17 22.549433210199382 27.988506693945826 26.320459184113382 23.14160091174141 18-19 22.31385000161281 27.284899104701992 28.431398965014964 21.969851928670238 20-21 23.325645559183457 26.313217942680495 28.256128953502756 22.10500754463329 22-23 23.44855844747229 26.066446986781212 27.9460446553088 22.538949910437697 24-25 22.948925134429427 26.31629102611767 28.256211537413073 22.47857230203983 26-27 22.809585491978627 26.549880852212276 27.95203827513196 22.688495380677136 28-29 22.535058362302973 26.56372247399758 28.210500275314406 22.690718888385042 30-31 23.022199565689043 26.42650022892004 28.020490509067127 22.530809696323793 32-33 22.95476909987548 26.693522350414206 27.704123130308684 22.647585419401633 34-35 23.090150335826657 26.55907530749705 28.001256946417058 22.349517410259235 36-37 23.05540081199566 26.377567973225823 27.837850613492776 22.72918060128574 38-39 22.99185804330038 26.47950221287654 28.044518376375944 22.48412136744714 40-41 22.96605425318283 26.125766421578845 28.24847233494378 22.659706990294545 42-43 22.807760580270628 26.175844533067256 27.989992955013843 23.026401931648273 44-45 22.805827275013844 26.60749855229043 27.851579748459475 22.735094424236248 46-47 22.868410380723528 26.475693826153567 27.52621282729386 23.129682965829048 48-49 23.13370820475603 26.431099569366296 27.505932041881394 22.929260183996288 50-51 23.014030677407604 26.520693292487923 27.302694886250485 23.16258114385399 52-53 22.83345048373052 26.791850490630175 27.20642659767833 23.168272427960975 54-55 22.793357323871806 26.691659420177157 27.23079593018054 23.284187325770496 56-57 23.0462030529204 26.622239570835116 27.048918943277496 23.28263843296699 58-59 22.786505699005605 26.582782172355056 27.442736896302456 23.18797523233688 60-61 22.877697686632743 26.846907865558002 27.151288264718364 23.12410618309089 62-63 22.888654334708576 26.65010996138839 27.121778945962998 23.339456757940034 64-65 22.616241542764005 26.532377515168953 27.255724271873667 23.595656670193378 66-67 22.815331120982478 26.425380010161042 27.145095334725895 23.61419353413059 68-69 22.562787095347467 26.531938444840215 27.252504382527455 23.652770077284867 70-71 22.680919855840845 26.248400808960632 27.317678746534526 23.753000588664 72-73 22.879392874224894 26.452017312019887 27.152202913450026 23.516386900305193 74-75 22.86346486224789 26.625296582190533 27.086330979565087 23.424907575996492 76-77 22.763772807457936 26.54197916595687 27.049669846682235 23.64457817990296 78-79 22.722197690280822 26.460291886082523 27.080799205604016 23.736711218032642 80-81 22.640333167031137 26.712378081098542 27.111984623297037 23.535304128573287 82-83 23.02740834290486 26.32078284582559 27.43395166635002 23.217857144919527 84-85 23.063792735306805 26.413601839816263 26.98586095933695 23.536744465539982 86-87 22.600890966963107 26.673358107397434 27.28823480404379 23.437516121595678 88-89 22.921135936003754 26.194195633166185 27.261310552218514 23.623357878611547 90-91 22.943942119889837 26.197703778359838 27.29851555152541 23.559838550224914 92-93 23.05468482200981 26.312326139413774 27.174777843897306 23.458211194679112 94-95 22.659002176330898 26.11025241425939 27.407240427284336 23.823504982125378 96-97 22.862265932324018 26.05845930901602 27.26808308374624 23.81119167491372 98-99 22.728804012833585 26.19258945590651 27.201158402473002 23.8774481287869 100 23.11765224858771 25.966951083118516 26.759513285331714 24.15588338296206 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1543.0 1 1176.5 2 1104.5 3 1435.0 4 1239.0 5 1142.0 6 1691.0 7 3015.0 8 4953.5 9 6236.5 10 6065.5 11 5626.5 12 6054.5 13 7071.5 14 8165.5 15 9359.0 16 10541.5 17 11564.0 18 12529.5 19 13545.5 20 14815.5 21 16350.0 22 18245.5 23 21068.5 24 24597.0 25 29179.5 26 36441.0 27 45888.5 28 57301.5 29 74896.0 30 100124.5 31 125823.5 32 159229.0 33 198901.0 34 235455.0 35 271689.5 36 315609.5 37 366558.0 38 391231.5 39 393730.0 40 390564.5 41 384587.0 42 391522.0 43 412487.5 44 437020.0 45 470617.5 46 502826.5 47 524317.0 48 541016.5 49 544099.5 50 533770.5 51 525255.0 52 517104.5 53 504475.5 54 488724.0 55 452238.5 56 407313.0 57 356197.5 58 308145.5 59 273700.0 60 230483.0 61 186261.5 62 151262.0 63 120519.0 64 90018.5 65 62906.5 66 44900.0 67 33658.0 68 25356.5 69 18868.5 70 14544.5 71 10946.5 72 7977.0 73 5941.0 74 4549.0 75 3263.0 76 2186.0 77 1395.5 78 900.0 79 581.5 80 392.0 81 289.5 82 224.5 83 177.0 84 133.5 85 101.5 86 77.5 87 67.0 88 55.0 89 42.5 90 34.5 91 29.5 92 22.5 93 19.0 94 16.0 95 13.0 96 8.5 97 6.0 98 5.0 99 2.5 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02601841790011744 2 0.1122462713667888 3 0.017314830013387825 4 0.017684213053673434 5 0.020015943495476328 6 0.04680390939618878 7 0.04708094667640299 8 0.03885447855004229 9 0.03340607870582958 10-11 0.03765398366911406 12-13 0.03669204866837029 14-15 0.03204782648477938 16-17 0.03842353166970907 18-19 0.03761550626908431 20-21 0.04824681189730444 22-23 0.04768504185687008 24-25 0.066527424651439 26-27 0.05862801442533118 28-29 0.05204068354023785 30-31 0.049878253658565866 32-33 0.044883887134704224 34-35 0.04633063737582285 36-37 0.041624851352184335 38-39 0.047073251196397034 40-41 0.055834555183171285 42-43 0.05687344498397455 44-45 0.05962073134609875 46-47 0.06166003354767553 48-49 0.061302193727398854 50-51 0.05491109758245726 52-53 0.06529614785048699 54-55 0.05189062168012183 56-57 0.05672723086386149 58-59 0.05371829818153499 60-61 0.04965508473839331 62-63 0.05660025544376332 64-65 0.05459173516221033 66-67 0.05231002534044611 68-69 0.058158590144968214 70-71 0.0585318209252568 72-73 0.06111365446725308 74-75 0.051351938079705325 76-77 0.052417762060529415 78-79 0.06287591938861566 80-81 0.06342614620904109 82-83 0.05308726882104708 84-85 0.059193632205768516 86-87 0.05696963848404893 88-89 0.06544620971060301 90-91 0.0706329632346134 92-93 0.07495397525795441 94-95 0.07828227036052784 96-97 0.07460383091768368 98-99 0.08386534110484467 100 0.12779114097880812 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1.2994641E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.846326633113755 #Duplication Level Percentage of deduplicated Percentage of total 1 69.91867718679508 34.85189220808227 2 15.422553847906556 15.375153132390713 3 6.046312143915045 9.041593501540547 4 2.995461276668044 5.97250964854557 5 1.6575321629676316 4.131094480008805 6 1.0061427754596433 3.0091512855065408 7 0.6618936539855641 2.309507709106472 8 0.4446178928422274 1.773005497083235 9 0.31790660941004145 1.4261829022330776 >10 1.4108763763351764 11.967867376583099 >50 0.06987049158715056 2.391812183230972 >100 0.04275883449904037 4.1290149924887505 >500 0.0036833762991790833 1.206629829151619 >1k 0.0016110805108330447 1.2681534665832968 >5k 2.5572704691747757E-5 0.09056781802749621 >10k+ 7.671811407524326E-5 1.055863969437518 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 37736 0.2903966335045347 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 28933 0.22265332301215557 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16163 0.1243820433361722 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 3.078192002380058E-5 7 0.0 0.0 0.0 7.695480005950145E-6 3.078192002380058E-5 8 0.0 0.0 0.0 7.695480005950145E-6 3.847740002975072E-5 9 0.0 0.0 0.0 1.539096001190029E-5 9.234576007140174E-5 10-11 0.0 0.0 0.0 1.9238700014875365E-5 9.234576007140174E-5 12-13 0.0 0.0 0.0 3.078192002380058E-5 9.234576007140174E-5 14-15 0.0 0.0 0.0 3.078192002380058E-5 1.0773672008330203E-4 16-17 0.0 0.0 0.0 3.462966002677565E-5 1.3851864010710262E-4 18-19 0.0 0.0 0.0 5.3868360041651014E-5 1.4621412011305276E-4 20-21 0.0 0.0 0.0 1.3467090010412755E-4 1.5006186011602782E-4 22-23 0.0 0.0 0.0 3.4629660026775657E-4 1.8853926014577855E-4 24-25 0.0 0.0 0.0 9.61935000743768E-4 2.1932118016957915E-4 26-27 0.0 0.0 0.0 0.0022624711217493427 2.2316892017255422E-4 28-29 0.0 0.0 0.0 0.00609866790471549 2.2316892017255422E-4 30-31 0.0 0.0 0.0 0.01868847319444993 2.2701666017552928E-4 32-33 0.0 0.0 0.0 0.04738491813663802 2.7318954021123015E-4 34-35 0.0 3.847740002975073E-6 0.0 0.0912722406105717 3.386011202618064E-4 36-37 0.0 7.695480005950145E-6 0.0 0.16169742588502445 3.6553530028263193E-4 38-39 0.0 7.695480005950145E-6 0.0 0.26491689920483374 3.809262602945322E-4 40-41 0.0 7.695480005950145E-6 0.0 0.3716724455873771 3.886217403004823E-4 42-43 0.0 1.539096001190029E-5 0.0 0.4813561221121846 4.1555592032130784E-4 44-45 0.0 1.539096001190029E-5 0.0 0.6147649634953363 4.4249010034213334E-4 46-47 0.0 1.539096001190029E-5 0.0 0.7765470396604262 4.6942428036295884E-4 48-49 0.0 1.539096001190029E-5 0.0 0.9473482183924897 5.3098812041056E-4 50-51 0.0 1.539096001190029E-5 0.0 1.1261334576307265 5.348358604135351E-4 52-53 0.0 1.539096001190029E-5 0.0 1.2991009139844647 5.733132604432859E-4 54-55 0.0 1.539096001190029E-5 0.0 1.490021925192085 6.079429204700615E-4 56-57 0.0 1.539096001190029E-5 0.0 1.7114401236632855 6.117906604730366E-4 58-59 0.0 1.539096001190029E-5 0.0 1.961123820196341 6.348771004908869E-4 60-61 0.0 1.539096001190029E-5 0.0 2.216471390013776 6.38724840493862E-4 62-63 0.0 1.539096001190029E-5 7.695480005950145E-6 2.4733465126123915 6.695067605176627E-4 64-65 0.0 1.539096001190029E-5 7.695480005950145E-6 2.7377516623968297 6.84897720529563E-4 66-67 0.0 1.539096001190029E-5 7.695480005950145E-6 3.030176054882932 7.002886805414632E-4 68-69 0.0 1.539096001190029E-5 7.695480005950145E-6 3.355667924954602 7.272228605622888E-4 70-71 0.0 1.539096001190029E-5 7.695480005950145E-6 3.70531590676495 7.772434806009647E-4 72-73 0.0 1.539096001190029E-5 7.695480005950145E-6 4.053101582413858 9.927169207675688E-4 74-75 0.0 1.539096001190029E-5 7.695480005950145E-6 4.394292231697667 0.001000412400773519 76-77 0.0 1.539096001190029E-5 7.695480005950145E-6 4.766149368805187 0.0010158033607854194 78-79 0.0 1.539096001190029E-5 7.695480005950145E-6 5.171578037438664 0.0010311943207973195 80-81 0.0 1.539096001190029E-5 7.695480005950145E-6 5.6047450637535885 0.0010465852808092197 82-83 0.0 2.3086440017850436E-5 7.695480005950145E-6 6.047123579635636 0.0010773672008330202 84-85 0.0 2.3086440017850436E-5 7.695480005950145E-6 6.473814859525554 0.0010889104208419455 86-87 0.0 2.3086440017850436E-5 7.695480005950145E-6 6.913084401485197 0.0011004536408508708 88 0.0 2.3086440017850436E-5 7.695480005950145E-6 7.263209503055913 0.0011004536408508708 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 20405 0.0 51.40333 1 GTATCAA 37650 0.0 42.61277 1 CAACGCA 46410 0.0 33.58319 5 ATCAACG 47155 0.0 33.182407 3 TATCAAC 48605 0.0 32.78426 2 AACGCAG 49975 0.0 31.639067 6 TCAACGC 50005 0.0 31.460098 4 GTACTAG 5615 0.0 29.653538 1 ACGCAGA 56060 0.0 27.753809 7 CGCAGAG 57740 0.0 26.822554 8 GTGGTAT 9465 0.0 26.437113 1 GTACATG 56855 0.0 26.009792 1 TACATGG 58615 0.0 25.027042 2 ACATGGG 59135 0.0 24.028606 3 GCAGAGT 62940 0.0 23.928444 9 GCATCGT 6105 0.0 22.089502 8 CATGGGG 40105 0.0 21.616526 4 GAGTACT 31605 0.0 21.4992 12-13 TAGGCAT 8135 0.0 21.313602 5 TGGTATC 11230 0.0 21.108322 2 >>END_MODULE