FastQCFastQC Report
Mon 6 Jun 2016
ERR1211342_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211342_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12994641
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT333310.25649804407832427No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT268980.20699302120004698No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154110.1185950423716977No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA206800.053.3308071
GTATCAA380150.041.604061
CAACGCA471650.032.653375
ATCAACG475950.032.3758163
TATCAAC495850.031.6045782
TCAACGC502750.030.7528694
AACGCAG508200.030.6093066
GTACTAG56850.026.6644671
ACGCAGA577450.026.5810747
CGCAGAG596150.025.684338
GTACATG572550.025.1069771
TACATGG587600.024.1704352
GCAGAGT656350.023.0772699
ACATGGG593200.023.0102583
GTGGTAT94800.022.574461
GAGTACT330650.021.20733512-13
TAGGCAT81800.021.0469785
CATGGGG400300.020.513264
GCATCGT70350.020.0046428
TAGTACT82600.019.9960824
Produced by FastQC (version 0.11.5)