##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211342_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 12994641 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.12265371548163 33.0 31.0 34.0 30.0 34.0 2 32.26006820811749 34.0 31.0 34.0 30.0 34.0 3 32.32398948151011 34.0 31.0 34.0 30.0 34.0 4 35.95825209792252 37.0 35.0 37.0 35.0 37.0 5 35.86386003276274 37.0 35.0 37.0 35.0 37.0 6 35.878069890503326 37.0 35.0 37.0 35.0 37.0 7 35.79452283445153 37.0 35.0 37.0 33.0 37.0 8 35.77701815694639 37.0 35.0 37.0 33.0 37.0 9 37.39125651874492 39.0 37.0 39.0 34.0 39.0 10-11 37.34554448252938 39.0 37.0 39.0 34.0 39.0 12-13 37.275261856022034 39.0 37.0 39.0 34.0 39.0 14-15 38.51689292532206 40.0 38.0 41.0 34.0 41.0 16-17 38.50421539156026 40.0 38.0 41.0 34.0 41.0 18-19 38.50767708780874 40.0 38.0 41.0 34.0 41.0 20-21 38.47561998827055 40.0 38.0 41.0 34.0 41.0 22-23 38.36701156268957 40.0 38.0 41.0 34.0 41.0 24-25 38.276046910414834 40.0 38.0 41.0 34.0 41.0 26-27 38.17701085393587 40.0 38.0 41.0 33.0 41.0 28-29 38.08317501807091 40.0 37.5 41.0 33.0 41.0 30-31 38.09594839903619 40.0 37.0 41.0 33.0 41.0 32-33 38.045576903586635 40.0 37.0 41.0 33.0 41.0 34-35 37.92127866402773 40.0 37.0 41.0 33.0 41.0 36-37 37.78191621453797 40.0 37.0 41.0 33.0 41.0 38-39 37.669761057654455 40.0 37.0 41.0 33.0 41.0 40-41 37.561801745811984 40.0 37.0 41.0 32.0 41.0 42-43 37.45754603763197 40.0 36.0 41.0 32.0 41.0 44-45 37.29400519798892 40.0 36.0 41.0 31.5 41.0 46-47 37.05348620250456 39.0 36.0 41.0 31.0 41.0 48-49 35.49658759330096 39.0 35.0 41.0 24.5 41.0 50-51 36.22732082402276 39.0 35.0 40.0 29.0 41.0 52-53 36.31818031756322 39.0 35.0 40.0 30.0 41.0 54-55 36.14153765386824 38.5 35.0 40.0 29.0 41.0 56-57 35.917180936356765 38.0 35.0 40.0 29.0 41.0 58-59 35.667464110782284 38.0 34.0 40.0 28.0 41.0 60-61 35.409494883313826 37.5 34.0 40.0 28.0 41.0 62-63 35.08512058932602 37.0 34.0 40.0 28.0 41.0 64-65 34.745606746658105 36.0 34.0 39.0 27.0 41.0 66-67 34.403128104885695 36.0 33.0 39.0 26.5 40.5 68-69 34.04711238271223 35.0 33.0 39.0 26.0 40.0 70-71 33.69752284807252 35.0 33.0 38.0 26.0 40.0 72-73 33.32981438271361 35.0 32.0 37.0 26.0 39.5 74-75 32.97656029897247 35.0 32.0 37.0 25.5 39.0 76-77 32.047250708965336 34.0 30.5 36.0 24.5 38.0 78-79 32.485345574379465 34.5 32.0 36.0 25.0 38.0 80-81 32.36867659522106 35.0 32.0 35.5 25.0 37.0 82-83 32.108595728038964 34.0 31.5 35.0 25.0 37.0 84-85 31.838884621745226 34.0 31.0 35.0 24.5 36.5 86-87 31.590448400998536 34.0 31.0 35.0 24.0 36.0 88-89 31.369089380768578 34.0 31.0 35.0 24.0 36.0 90-91 31.159038021904568 34.0 31.0 35.0 23.0 35.0 92-93 30.976029503239065 34.0 30.0 35.0 23.0 35.0 94-95 30.786156385543855 34.0 30.0 35.0 20.5 35.0 96-97 30.600449446814267 34.0 30.0 35.0 20.0 35.0 98-99 30.388793080162813 34.0 29.0 35.0 20.0 35.0 100 27.91941177905569 31.0 25.0 34.0 15.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.29399580296648864 1101 2 -0.29344407682447127 1101 3 -0.29861405557963394 1101 4 -0.16045498526241886 1101 5 -0.13716756421942478 1101 6 -0.2206778094398203 1101 7 -0.23222026485208147 1101 8 -0.1904352812832144 1101 9 -0.31342597257013693 1101 10-11 -0.31167285583723725 1101 12-13 -0.4503111623724081 1101 14-15 -0.489845201815335 1101 16-17 -0.5192895790635035 1101 18-19 -0.547062879549955 1101 20-21 -0.5522237695257601 1101 22-23 -0.6148028095663136 1101 24-25 -0.5758147366145323 1101 26-27 -0.634085447393538 1101 28-29 -0.6289365332133485 1101 30-31 -0.5570745997611297 1101 32-33 -0.5351463988206859 1101 34-35 -0.5556440564296139 1101 36-37 -0.5650454187148668 1101 38-39 -0.5573234352905843 1101 40-41 -0.5398089024332506 1101 42-43 -0.5373604622701578 1101 44-45 -0.5020450169491681 1101 46-47 -0.5940274476055052 1101 48-49 0.6207977899990738 1101 50-51 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0.999223309095818 2201 68-69 0.9643416273431029 2201 70-71 0.9303059508033868 2201 72-73 0.9653982589488095 2201 74-75 0.9127615185891997 2201 76-77 0.9585687792169679 2201 78-79 0.7741519410504267 2201 80-81 0.7453733829864646 2201 82-83 0.7149234243233522 2201 84-85 0.19027869059197045 2201 86-87 -0.017528777144267593 2201 88-89 0.2848774809123107 2201 90-91 0.4976317178309557 2201 92-93 0.6352198893668373 2201 94-95 0.7156159915451816 2201 96-97 0.7088173779367466 2201 98-99 0.7252367054832938 2201 100 1.0900046178212435 2202 1 0.5623150383726454 2202 2 0.5304524765406669 2202 3 0.501121771286563 2202 4 0.34074507994032643 2202 5 0.4061141896267557 2202 6 0.40707431414467266 2202 7 0.46459112735653463 2202 8 0.37693457899029426 2202 9 0.5293402994426444 2202 10-11 0.5798250148641202 2202 12-13 0.5942166565573359 2202 14-15 0.7238844835769029 2202 16-17 0.742559183746998 2202 18-19 0.7048601454681602 2202 20-21 0.7103438041498364 2202 22-23 0.6746705215487481 2202 24-25 0.6922277727475503 2202 26-27 0.5949829550147072 2202 28-29 0.6331090881780739 2202 30-31 0.7414764105641254 2202 32-33 0.7798870558173192 2202 34-35 0.8174753564293411 2202 36-37 0.8204870291291257 2202 38-39 0.9058806954891594 2202 40-41 0.8747803349399845 2202 42-43 0.8828646118012955 2202 44-45 0.8920801249978041 2202 46-47 0.9034148753841791 2202 48-49 2.140798947403866 2202 50-51 1.328929375859964 2202 52-53 0.8235150841979859 2202 54-55 0.819586798430187 2202 56-57 0.7585625153645665 2202 58-59 0.7949111377055331 2202 60-61 0.8869112968454758 2202 62-63 0.9490845465314948 2202 64-65 0.9625453868201106 2202 66-67 0.972263605002091 2202 68-69 0.9592731098056078 2202 70-71 0.9415412858549104 2202 72-73 0.9582149695049154 2202 74-75 0.9009841233527709 2202 76-77 0.9849007910296024 2202 78-79 0.8303910142917701 2202 80-81 0.775106986505989 2202 82-83 0.7294198519592285 2202 84-85 0.8080712003538189 2202 86-87 0.8649956304379351 2202 88-89 0.7906815733830506 2202 90-91 0.8361997592210209 2202 92-93 0.8244539055654734 2202 94-95 0.8081931588356319 2202 96-97 0.7733247625852826 2202 98-99 0.16597494122624923 2202 100 0.7313600516827847 2203 1 0.5701071490427765 2203 2 0.518909187646095 2203 3 0.5470843193662631 2203 4 0.34501723664064343 2203 5 0.3798149788006526 2203 6 0.3830701941909851 2203 7 0.4552486964742073 2203 8 0.4441080337045875 2203 9 0.572627784816909 2203 10-11 0.5596157486129201 2203 12-13 0.618633232282491 2203 14-15 0.725378078753856 2203 16-17 0.7246363360108532 2203 18-19 0.7167561922708217 2203 20-21 0.6794725091526956 2203 22-23 0.6930657256125485 2203 24-25 0.7154530551681475 2203 26-27 0.7551767034891981 2203 28-29 0.6218053629001332 2203 30-31 0.7183073754028229 2203 32-33 0.783837891301701 2203 34-35 0.8516379196279971 2203 36-37 0.866647955573562 2203 38-39 0.8765867705444919 2203 40-41 0.9337659357188244 2203 42-43 0.9479939914044948 2203 44-45 0.9189224609040636 2203 46-47 0.9653118738779156 2203 48-49 2.1674806733753726 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1.046417987456664 2204 76-77 1.105851433309951 2204 78-79 0.9504333468062001 2204 80-81 0.9213087764033077 2204 82-83 0.8709309514171863 2204 84-85 0.9060531113780179 2204 86-87 0.9698188315435807 2204 88-89 0.747633374374967 2204 90-91 0.05178076476645188 2204 92-93 0.6598361944207909 2204 94-95 0.87994789309532 2204 96-97 0.9775012397887863 2204 98-99 1.0017664068821368 2204 100 1.2659287113014699 2205 1 0.47888940432115845 2205 2 0.5003049948759681 2205 3 0.47357781741376215 2205 4 0.3175692996927424 2205 5 0.05398530959761416 2205 6 0.2728814645593616 2205 7 0.379721193009523 2205 8 0.38739024427668767 2205 9 0.5651887374904021 2205 10-11 0.5773731181341972 2205 12-13 0.6022449879780112 2205 14-15 0.7296335931658078 2205 16-17 0.7147114996782804 2205 18-19 0.6977187885808149 2205 20-21 0.6881764909989911 2205 22-23 0.6757566570623652 2205 24-25 0.711213317046024 2205 26-27 0.7245257885997205 2205 28-29 0.7732276802475013 2205 30-31 0.816723796779975 2205 32-33 0.8222512422649402 2205 34-35 0.8301121691334501 2205 36-37 0.8446259777789678 2205 38-39 0.8510819422271894 2205 40-41 0.891747065481411 2205 42-43 0.9034665759127165 2205 44-45 0.8721289943294863 2205 46-47 0.9404007331404998 2205 48-49 2.1770790520824193 2205 50-51 1.3280185393577497 2205 52-53 1.0033160383682471 2205 54-55 0.9568940411835953 2205 56-57 0.9738096712793194 2205 58-59 0.9526885228956985 2205 60-61 0.9379614824925113 2205 62-63 0.9672650119126018 2205 64-65 0.9995405526602354 2205 66-67 0.9660442164837235 2205 68-69 1.0226113484683879 2205 70-71 1.0216548766846003 2205 72-73 1.0211143555409947 2205 74-75 0.9576701015204776 2205 76-77 0.9879856665614071 2205 78-79 0.8554400767213295 2205 80-81 0.7349405482665219 2205 82-83 0.7853142695157587 2205 84-85 0.8179543990840479 2205 86-87 0.8839413764937554 2205 88-89 0.8969226959618304 2205 90-91 0.9523736136993044 2205 92-93 0.8681249852986355 2205 94-95 0.8729738643100013 2205 96-97 0.9383135796428874 2205 98-99 0.9675175579751105 2205 100 1.2241630985178915 2206 1 0.5573311717385891 2206 2 0.5466136625917883 2206 3 0.5073747114423028 2206 4 0.33561104979829537 2206 5 0.40133997387340514 2206 6 0.40673808990622007 2206 7 0.46525194823404803 2206 8 0.4750531338789372 2206 9 0.5903510121078739 2206 10-11 0.6144470055313391 2206 12-13 0.6088217779224863 2206 14-15 0.7844183498262467 2206 16-17 0.7686388747770891 2206 18-19 0.738208708245196 2206 20-21 0.7255263626282371 2206 22-23 0.7327819234062005 2206 24-25 0.7479534687392828 2206 26-27 0.756856627913244 2206 28-29 0.7813424912106512 2206 30-31 0.8296672540946801 2206 32-33 0.8337717104523534 2206 34-35 0.8943369992125696 2206 36-37 0.8856785145414534 2206 38-39 0.8847920199134975 2206 40-41 0.9353207662372185 2206 42-43 0.9460373019217343 2206 44-45 0.9728372241209513 2206 46-47 0.9702740552217435 2206 48-49 2.223788392491187 2206 50-51 1.3848321367439596 2206 52-53 1.1286546582645727 2206 54-55 1.0746056627029361 2206 56-57 1.0348762321689193 2206 58-59 1.0293471461165993 2206 60-61 1.0314544587049852 2206 62-63 1.0565826982695512 2206 64-65 1.0075905059251937 2206 66-67 1.0293693678470603 2206 68-69 1.0702556798522735 2206 70-71 0.9930985831621157 2206 72-73 1.0470316554143793 2206 74-75 1.0407112675213313 2206 76-77 0.9950075817734998 2206 78-79 0.8951381148661213 2206 80-81 0.8610872319897851 2206 82-83 0.8943204282326249 2206 84-85 0.9580608589678867 2206 86-87 0.9320826250933756 2206 88-89 0.9276533353703265 2206 90-91 0.9756806231782598 2206 92-93 0.9977128787896845 2206 94-95 0.967592157985468 2206 96-97 1.0461399644111253 2206 98-99 1.1534415518328949 2206 100 1.2876549928329695 2207 1 0.5524793540348867 2207 2 0.5151226121768886 2207 3 0.5063115610564495 2207 4 0.35965087222433567 2207 5 0.38162336078979564 2207 6 0.3996096736950392 2207 7 0.4475853645951986 2207 8 0.4666308809834163 2207 9 0.5930680151179288 2207 10-11 0.605833184734685 2207 12-13 0.6005944277027737 2207 14-15 0.7457355970014206 2207 16-17 0.7443722690430334 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0.9984882392192027 2207 86-87 1.064797579732062 2207 88-89 1.06642264329653 2207 90-91 1.1178454303509717 2207 92-93 1.070313690052803 2207 94-95 1.100284481799914 2207 96-97 1.1265384988614926 2207 98-99 1.1757134732723102 2207 100 1.3819894714782102 2208 1 0.5618267252541074 2208 2 0.5274311183728884 2208 3 0.5007603011012591 2208 4 0.33145075958437786 2208 5 0.38077417670645275 2208 6 0.43714138390703283 2208 7 0.4689171016200788 2208 8 0.4878927580216441 2208 9 0.6292126483635911 2208 10-11 0.6152938107407664 2208 12-13 0.6236780711859282 2208 14-15 0.8241509289680735 2208 16-17 0.8062140687107089 2208 18-19 0.7515104525129459 2208 20-21 0.7492175932452909 2208 22-23 0.764196720893203 2208 24-25 0.8075555957250842 2208 26-27 0.8242104208930456 2208 28-29 0.8588235375763631 2208 30-31 0.8925755210463464 2208 32-33 0.9313462738033849 2208 34-35 0.9320834683074608 2208 36-37 0.9781689651557954 2208 38-39 0.9804717048631844 2208 40-41 0.9970700951675724 2208 42-43 0.9523394146806652 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1.347513890647825 2311 1 0.3347334636098722 2311 2 0.36922271270757534 2311 3 0.38850230010797304 2311 4 0.2628401684380819 2311 5 0.32493186919889894 2311 6 0.36997808718897573 2311 7 0.402035489872425 2311 8 0.16533184288295644 2311 9 0.43686163422027846 2311 10-11 0.3741171367777838 2311 12-13 0.4677123183149732 2311 14-15 0.4488514488762263 2311 16-17 0.5078196831499255 2311 18-19 0.6105053100817912 2311 20-21 0.6376090334938027 2311 22-23 0.6768997486700883 2311 24-25 0.6192603370024514 2311 26-27 0.6863444630861508 2311 28-29 0.7193298772111376 2311 30-31 0.5904300192200154 2311 32-33 0.6044401121967695 2311 34-35 0.7298661128992379 2311 36-37 0.7839199234160432 2311 38-39 0.8121773057738508 2311 40-41 0.7849995663674321 2311 42-43 0.7763560735561867 2311 44-45 0.8155679002046767 2311 46-47 0.8644607617995632 2311 48-49 -6.228323361440939 2311 50-51 -1.098267257070738 2311 52-53 0.38162671190580255 2311 54-55 0.7814471855456802 2311 56-57 0.8797718826353744 2311 58-59 0.9107526366977297 2311 60-61 0.8025337368249197 2311 62-63 0.7668300743953935 2311 64-65 0.8220747345227863 2311 66-67 0.9608991161460168 2311 68-69 0.9205503089916007 2311 70-71 0.9813469858748647 2311 72-73 0.39545495193946323 2311 74-75 0.7481782235875585 2311 76-77 0.8844184893731537 2311 78-79 0.7883600091108534 2311 80-81 0.806633267121974 2311 82-83 0.8308166240437416 2311 84-85 0.849974161580743 2311 86-87 0.9124618261472683 2311 88-89 0.8876021026715044 2311 90-91 1.0192793469282542 2311 92-93 0.9861375794722775 2311 94-95 0.980618999325241 2311 96-97 1.0277678155548173 2311 98-99 1.0601817936583693 2311 100 1.2338237011967017 2312 1 0.06825506448397789 2312 2 0.23935382906570624 2312 3 0.21486328770280494 2312 4 -0.017072820046770687 2312 5 0.16807969511287268 2312 6 0.09742429429220323 2312 7 0.2369711947919626 2312 8 0.31793883143301116 2312 9 0.23164034468639727 2312 10-11 0.22295459334754497 2312 12-13 0.24327553918602263 2312 14-15 0.27768926784430903 2312 16-17 0.44514063673548776 2312 18-19 0.31621506604726335 2312 20-21 0.28587162363520946 2312 22-23 0.5645561670621291 2312 24-25 0.6835543376386468 2312 26-27 0.5847197098347436 2312 28-29 0.7319032041603677 2312 30-31 0.7517321062659761 2312 32-33 0.7498525431315812 2312 34-35 0.25286093350423755 2312 36-37 0.6227780007727546 2312 38-39 0.7580105331966251 2312 40-41 0.554582409058554 2312 42-43 0.6015180453456708 2312 44-45 0.7226059266101146 2312 46-47 0.7207237554141699 2312 48-49 -4.797743525504693 2312 50-51 -0.9060770998092025 2312 52-53 0.42748946995688897 2312 54-55 0.6799163841747742 2312 56-57 0.8218877321237485 2312 58-59 0.8442883996562571 2312 60-61 0.8617022094969471 2312 62-63 0.5921164749815659 2312 64-65 0.7612443385959367 2312 66-67 0.7781224211118953 2312 68-69 0.7318407665970739 2312 70-71 0.7247415005180642 2312 72-73 0.8176895626278835 2312 74-75 0.49641985494917407 2312 76-77 0.6735058838240633 2312 78-79 0.38522684766576276 2312 80-81 0.5820932735429167 2312 82-83 0.5399677665240219 2312 84-85 0.6120947392851157 2312 86-87 0.7618616125578832 2312 88-89 0.7891756896081468 2312 90-91 0.5878832122218007 2312 92-93 0.7410037623947439 2312 94-95 0.8431796170067045 2312 96-97 0.978910604362337 2312 98-99 1.001877896554813 2312 100 0.5241023040024295 2313 1 0.05387551736625085 2313 2 0.19738750188088972 2313 3 0.29110364030931635 2313 4 0.039697494228605024 2313 5 0.09788808479729028 2313 6 0.23323103901158504 2313 7 0.17120526588093554 2313 8 0.2342466330469577 2313 9 0.4332784269872505 2313 10-11 0.22126256000209565 2313 12-13 -0.008433716870833052 2313 14-15 0.15263272557169927 2313 16-17 0.06792774162422432 2313 18-19 0.40558748332067296 2313 20-21 0.38695548012606196 2313 22-23 0.4821954064607681 2313 24-25 0.25277474527078425 2313 26-27 0.38121534260672973 2313 28-29 0.36700584444713513 2313 30-31 0.5153258583913285 2313 32-33 0.4574544103573288 2313 34-35 0.3844194857355063 2313 36-37 -0.33402530121798435 2313 38-39 0.14854848165136048 2313 40-41 0.3734127858679983 2313 42-43 0.46684740334682573 2313 44-45 0.47105491909169217 2313 46-47 0.4287855576755888 2313 48-49 -5.467069740480042 2313 50-51 -1.39288034285687 2313 52-53 0.07101749602605167 2313 54-55 0.24456806464167613 2313 56-57 0.2548385105803277 2313 58-59 0.32059891038435495 2313 60-61 0.6268796031976578 2313 62-63 0.720774237012975 2313 64-65 0.8131120177533973 2313 66-67 0.8474083484994779 2313 68-69 0.8760457515484532 2313 70-71 0.8483790405426674 2313 72-73 0.6529413130103379 2313 74-75 0.7357098675448555 2313 76-77 0.7262148029986051 2313 78-79 0.6585805586760571 2313 80-81 -0.20800581429041642 2313 82-83 0.20966196988123897 2313 84-85 0.545952832939296 2313 86-87 0.7171075593150711 2313 88-89 0.7504286514973693 2313 90-91 0.8218052137652023 2313 92-93 0.8973409471286686 2313 94-95 0.7500313974047828 2313 96-97 0.7659584135391384 2313 98-99 0.9450740776787008 2313 100 0.9780273728524698 2314 1 0.021811011148322734 2314 2 0.16354628154043382 2314 3 0.18453615929760758 2314 4 0.0538258305497763 2314 5 0.16906109944193304 2314 6 0.2334506632292559 2314 7 0.0707585691001924 2314 8 0.2029885653915997 2314 9 0.38648644486504935 2314 10-11 0.32364167014205947 2314 12-13 0.2030281029328549 2314 14-15 0.21745253657437047 2314 16-17 0.46269805881512127 2314 18-19 0.5417394574979681 2314 20-21 0.584314327884762 2314 22-23 0.2992470565242016 2314 24-25 0.3986768385440129 2314 26-27 0.451861537555196 2314 28-29 0.585188066887099 2314 30-31 0.5096157661714997 2314 32-33 0.4895500200675684 2314 34-35 0.387043245203607 2314 36-37 0.47474701860244295 2314 38-39 0.49845062236346394 2314 40-41 0.20767444757169073 2314 42-43 0.31322560106448805 2314 44-45 0.5479432242805586 2314 46-47 0.6601616128855667 2314 48-49 -6.498156303332436 2314 50-51 -1.5792392147783616 2314 52-53 -0.813267506043303 2314 54-55 -0.3675875153328292 2314 56-57 -0.07957992327647645 2314 58-59 0.3859458957663051 2314 60-61 0.6219220487909283 2314 62-63 0.5721120063975746 2314 64-65 0.688946537243126 2314 66-67 0.7587716240334998 2314 68-69 0.7556537735051165 2314 70-71 0.5526875292844693 2314 72-73 0.7338022663675119 2314 74-75 0.7795795268207399 2314 76-77 0.7458762712669937 2314 78-79 0.537420476664451 2314 80-81 0.5897744524268518 2314 82-83 0.6450556736796074 2314 84-85 0.6809716765881113 2314 86-87 0.7373451038970735 2314 88-89 0.6142686459643123 2314 90-91 0.7672286865606743 2314 92-93 0.783949298718948 2314 94-95 0.8770536952237649 2314 96-97 0.8729171787453467 2314 98-99 0.9409426312299765 2314 100 -0.5039143309875733 2315 1 0.10365857593608752 2315 2 0.18163840181829016 2315 3 0.2765061123112602 2315 4 0.10779449617629666 2315 5 0.20134912663785798 2315 6 0.25334566147262905 2315 7 0.30599982365082923 2315 8 0.25776684468441147 2315 9 0.42798057814194834 2315 10-11 0.1914041805850104 2315 12-13 0.37956511799282566 2315 14-15 0.20808325944760497 2315 16-17 0.34216501207497885 2315 18-19 0.41550733672217177 2315 20-21 0.332387189224562 2315 22-23 0.38682700817543036 2315 24-25 0.3977278789658314 2315 26-27 0.4895558602228931 2315 28-29 0.5658652824862287 2315 30-31 0.5634093395404633 2315 32-33 0.641736818972376 2315 34-35 0.6482038637787682 2315 36-37 0.5681126913564469 2315 38-39 0.6796825114579832 2315 40-41 0.6774574175457957 2315 42-43 0.705522375957429 2315 44-45 0.7643003889720532 2315 46-47 0.6177123605936501 2315 48-49 -4.803699018443314 2315 50-51 -0.8692983203019153 2315 52-53 0.2953808446626667 2315 54-55 0.2151292864697254 2315 56-57 0.5886870472038694 2315 58-59 0.7475592740112305 2315 60-61 0.5435476067278486 2315 62-63 0.3394666540622282 2315 64-65 0.5070842849000314 2315 66-67 -0.14269644855811947 2315 68-69 0.5827006472948355 2315 70-71 0.5711242408765571 2315 72-73 0.6695018348870789 2315 74-75 0.7399647998104371 2315 76-77 0.730249239733439 2315 78-79 0.6313796900408306 2315 80-81 0.5064088133876581 2315 82-83 0.49336646217742697 2315 84-85 0.5876836473174301 2315 86-87 0.5673200010582526 2315 88-89 0.5589481892686585 2315 90-91 0.5965805772181803 2315 92-93 0.6529496873902154 2315 94-95 0.3002725762792906 2315 96-97 0.4446831761643466 2315 98-99 0.40950346519957037 2315 100 0.46342960574061465 2316 1 0.23758819847407864 2316 2 0.27395561475169217 2316 3 0.19396303664103698 2316 4 -0.010335142490191629 2316 5 0.1600824631072868 2316 6 0.13065702093275178 2316 7 0.24266989953058982 2316 8 0.30040749777143816 2316 9 0.4392269061515037 2316 10-11 0.36949888364832617 2316 12-13 0.262481126423765 2316 14-15 0.397756324785945 2316 16-17 0.3753039073968907 2316 18-19 0.07243668511014789 2316 20-21 0.3860336256534609 2316 22-23 0.540267972097908 2316 24-25 0.58725512441125 2316 26-27 0.5707424167229576 2316 28-29 0.604893046335377 2316 30-31 0.533115409714604 2316 32-33 0.4779346257894943 2316 34-35 0.4515890542846037 2316 36-37 0.31595409385003137 2316 38-39 0.4701507970743961 2316 40-41 0.07005751245580427 2316 42-43 -0.021707033584448254 2316 44-45 0.2855254845713162 2316 46-47 0.27703418264185586 2316 48-49 -5.36198069233334 2316 50-51 -1.2253849395583742 2316 52-53 0.0792448676340669 2316 54-55 0.38273589481188 2316 56-57 0.36086739018259806 2316 58-59 0.21757568424165896 2316 60-61 0.3829828176355363 2316 62-63 0.33691557272911155 2316 64-65 0.32330237339034795 2316 66-67 0.3978978064913363 2316 68-69 0.04691640464847069 2316 70-71 -0.3182369465117816 2316 72-73 -0.191346264810484 2316 74-75 5.54203817614507E-4 2316 76-77 0.06110344103502996 2316 78-79 0.19449276684142802 2316 80-81 0.11476187225392209 2316 82-83 0.021839881257172067 2316 84-85 0.1879910322054421 2316 86-87 0.26513910840099086 2316 88-89 0.07098224624774829 2316 90-91 0.11303437699596586 2316 92-93 0.1964288974797661 2316 94-95 0.3725809564473046 2316 96-97 0.4621519039359683 2316 98-99 0.40965702194707276 2316 100 -0.38686577323774785 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 2.0 10 12.0 11 26.0 12 56.0 13 260.0 14 934.0 15 2695.0 16 6386.0 17 12958.0 18 22976.0 19 35201.0 20 51326.0 21 73435.0 22 93324.0 23 99395.0 24 102556.0 25 115153.0 26 137093.0 27 169475.0 28 209129.0 29 257379.0 30 316712.0 31 393355.0 32 497660.0 33 649343.0 34 903605.0 35 1282421.0 36 2011318.0 37 2941904.0 38 2307967.0 39 300568.0 40 16.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.96735265900774 20.09774326642915 14.26752554333397 23.66737853122914 2 15.328514955962621 20.145337812795898 40.228926024840206 24.29722120640127 3 18.73163039977652 25.539109024609 29.614383166591445 26.11487740902303 4 12.843152362612436 16.710943728746972 37.986163142282315 32.45974076635828 5 13.627292369732388 37.7702867896354 34.39057693066885 14.211843909963365 6 31.489157297032822 36.98951059660146 17.400561677824346 14.120770428541372 7 28.139543742475258 32.130244520142355 21.688119750958762 18.042091986423628 8 26.46565705243395 35.35227962024107 19.52128721428078 18.660776113044207 9 26.44327390855387 15.944061584407152 20.4349106681149 37.177753838924076 10-11 25.465073685020585 26.211866558928776 27.293343337565894 21.029716418484742 12-13 25.78870383164119 23.614147462095392 27.92529451113547 22.671854195127946 14-15 23.201502129111567 25.089879490146 25.434144987056957 26.274473393685483 16-17 22.48684528877569 28.107902492299868 26.321396668027237 23.083855550897205 18-19 22.24371527366016 27.323094546912767 28.474704068346956 21.958486111080116 20-21 23.258092264559703 26.339476177547777 28.30332555781495 22.09910600007757 22-23 23.29596102751723 26.134100503020314 28.03819077371689 22.53174769574557 24-25 22.847590015496777 26.36354419274874 28.322071315956293 22.466794475798192 26-27 22.73566937787645 26.584393960597335 28.040091485486368 22.63984517603984 28-29 22.47606196013155 26.604963567236407 28.29438026978368 22.62459420284836 30-31 22.974894605703625 26.434619520452777 28.100553942925277 22.48993193091832 32-33 22.918166945615614 26.720088333961726 27.773821928442686 22.58792279197997 34-35 23.003729182840562 26.561036568205072 28.105849049393385 22.329385199560985 36-37 22.99406143584818 26.40832210258407 27.932541765956394 22.665074695611352 38-39 22.950133115648956 26.460443791182637 28.151894043815073 22.437529049353333 40-41 22.835899726097693 26.121588428672233 28.410755569722724 22.63175627550735 42-43 22.74668278697072 26.171892105296575 28.11587533135575 22.965549776376957 44-45 22.73329850034198 26.589867078197756 27.929167119780313 22.74766730167995 46-47 22.809784811586468 26.462293646017287 27.622521122918315 23.105400419477927 48-49 23.2007962979328 26.473007655413657 27.528709213031078 22.79748683362247 50-51 22.981504645649736 26.516904343091475 27.347521400999685 23.154069610259103 52-53 22.723900211471555 26.753201350871798 27.331031439253895 23.191866998402755 54-55 22.77281577637584 26.67899404470042 27.2624908789537 23.285699299970037 56-57 22.996101027198073 26.592777701521996 27.088365878133153 23.322755393146778 58-59 22.684032885417178 26.61616362376367 27.473454983266492 23.226348507552657 60-61 22.80042074962419 26.868032671509994 27.17398657267524 23.15756000619057 62-63 22.81043286112444 26.6861049863977 27.105635295157526 23.397826857320332 64-65 22.489277865254355 26.572929335472793 27.251444325679497 23.68634847359335 66-67 22.690786060573707 26.490536274870657 27.12305627344964 23.69562139110599 68-69 22.452360499130283 26.616307114392036 27.186618228486477 23.744714157991208 70-71 22.514632307185924 26.335947581408707 27.251900452586213 23.89751965881915 72-73 22.725446010372615 26.56680737903219 27.024475361378204 23.68327124921699 74-75 22.68329882079643 26.770236858158015 26.943417807853344 23.603046513192215 76-77 22.544668524016476 26.701300714416227 26.904256896138495 23.8497738654288 78-79 22.48898381840858 26.626191042682535 26.910248466935332 23.97457667197355 80-81 22.36737423495125 26.95407283364129 26.85659910098836 23.8219538304191 82-83 22.700698941111316 26.5573724312116 27.220247206792752 23.521681420884324 84-85 22.774698094903737 26.66590950009533 26.710588998875178 23.84880340612575 86-87 22.264899166626385 26.943827717931956 27.008119596672664 23.783153518768994 88-89 22.478798911019187 26.520368660905476 26.9527370456051 24.048095382470237 90-91 22.448384400673604 26.530560642813743 27.00617689593305 24.0148780605796 92-93 22.553478095594627 26.66646553029233 26.843370615653555 23.936685758459483 94-95 22.103865196896983 26.48788899174614 27.08097089701642 24.32727491434046 96-97 22.247681137152725 26.458206521348064 26.9880915922206 24.306020749278606 98-99 21.99824600825259 26.64809437059008 26.895326469016194 24.45833315214114 100 22.150499855312436 26.46465270096156 26.691172072428614 24.69367537129739 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1338.0 1 955.5 2 762.5 3 979.5 4 818.0 5 766.0 6 1295.0 7 2488.0 8 4249.0 9 5463.0 10 5200.0 11 4831.5 12 5381.0 13 6392.5 14 7522.0 15 8753.5 16 10062.5 17 11288.0 18 12660.0 19 14017.0 20 15357.0 21 17039.5 22 19138.5 23 22048.0 24 25671.0 25 30268.5 26 37535.5 27 46903.0 28 58513.0 29 76302.5 30 101207.5 31 126682.0 32 160538.5 33 199703.0 34 236106.0 35 272739.5 36 316802.0 37 366767.0 38 391132.0 39 394290.0 40 390051.0 41 384624.5 42 392618.0 43 412860.0 44 438884.5 45 476089.0 46 509376.0 47 529738.0 48 547649.5 49 551919.5 50 540219.0 51 528514.0 52 519334.5 53 506381.0 54 490939.5 55 455543.0 56 408168.0 57 355833.5 58 307294.5 59 271897.5 60 226868.0 61 180932.5 62 145112.0 63 113777.5 64 83276.5 65 56813.0 66 39835.0 67 29719.0 68 22270.0 69 16272.0 70 12260.5 71 9098.5 72 6670.0 73 4916.0 74 3633.0 75 2430.5 76 1498.0 77 833.0 78 463.0 79 273.0 80 166.0 81 98.5 82 56.5 83 37.5 84 25.5 85 20.0 86 14.0 87 6.0 88 6.5 89 8.0 90 4.5 91 3.5 92 3.5 93 2.5 94 1.5 95 0.5 96 0.5 97 0.0 98 1.0 99 1.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02981228954305086 2 0.0017314830013387828 3 4.617288003570087E-5 4 0.02280170725763028 5 4.925107203808093E-4 6 2.3086440017850436E-5 7 4.617288003570087E-5 8 0.003870826442992923 9 0.010404288968044597 10-11 0.0010850626808389706 12-13 1.0388898008032697E-4 14-15 1.4621412011305276E-4 16-17 4.386423603391583E-4 18-19 1.539096001190029E-5 20-21 6.92593200535513E-5 22-23 2.8088502021718034E-4 24-25 2.693418002082551E-5 26-27 8.849802006842668E-5 28-29 0.0012004948809282226 30-31 0.0026318541620349496 32-33 0.009419267527282978 34-35 0.01834987207418812 36-37 0.021393434416541405 38-39 0.02110485391631827 40-41 0.004555724163522486 42-43 0.0052021444840222985 44-45 0.009326921767211576 46-47 0.012201183549433954 48-49 1.206131820032581 50-51 0.018719255114473726 52-53 0.019904359035390052 54-55 0.010311943207973195 56-57 0.008391920946488633 58-59 0.008468875746548135 60-61 0.0053098812041056 62-63 0.00817644750632203 64-65 0.019946684175422776 66-67 0.03005469716323829 68-69 0.030200911283351343 70-71 0.02712656702097426 72-73 0.024063765978606107 74-75 0.009965646607705438 76-77 0.014890753811513531 78-79 0.00878439042679209 80-81 0.0028550230822075038 82-83 0.014740691951397501 84-85 0.02481022753918327 86-87 0.023925247338499 88-89 0.033317580685761156 90-91 0.021235677076419424 92-93 0.01383647305069836 94-95 0.006960561665381906 96-97 0.003866978702989948 98-99 0.015325548431849714 100 0.03526068938726356 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1.2994641E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.266607126735856 #Duplication Level Percentage of deduplicated Percentage of total 1 68.99401272458846 33.99100918999314 2 15.849546425099504 15.61706753724676 3 6.304027100153912 9.317340793787361 4 3.0860974295219905 6.081661984603573 5 1.716022015476212 4.227129122864799 6 1.0388836484109119 3.0709363533990226 7 0.6611887490303323 2.280216843456673 8 0.45188340165097696 1.781020961298533 9 0.33160075507910036 1.4703159711069897 >10 1.4445816700390999 12.105768777891877 >50 0.07200644797040348 2.4176907812533446 >100 0.044900396521121766 4.2414972825866215 >500 0.0036041795518223286 1.1835014928204888 >1k 0.001540608854274313 1.1551166217344868 >5k 2.611201296449997E-5 0.08665529113093066 >10k+ 7.833603889349992E-5 0.9730709948253556 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 33331 0.25649804407832427 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 26898 0.20699302120004698 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15411 0.1185950423716977 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 7.695480005950145E-6 2 0.0 0.0 0.0 0.0 1.539096001190029E-5 3 0.0 0.0 0.0 0.0 1.539096001190029E-5 4 0.0 0.0 0.0 0.0 1.539096001190029E-5 5 0.0 0.0 0.0 7.695480005950145E-6 1.539096001190029E-5 6 0.0 0.0 0.0 7.695480005950145E-6 3.078192002380058E-5 7 0.0 0.0 0.0 7.695480005950145E-6 3.847740002975072E-5 8 0.0 0.0 0.0 7.695480005950145E-6 6.156384004760116E-5 9 0.0 0.0 0.0 7.695480005950145E-6 6.156384004760116E-5 10-11 0.0 0.0 0.0 2.3086440017850436E-5 6.156384004760116E-5 12-13 0.0 0.0 0.0 3.847740002975072E-5 6.156384004760116E-5 14-15 0.0 0.0 0.0 3.847740002975072E-5 1.1158446008627711E-4 16-17 0.0 0.0 0.0 4.23251400327258E-5 1.3851864010710262E-4 18-19 0.0 0.0 0.0 6.156384004760116E-5 1.6160508012495305E-4 20-21 0.0 0.0 0.0 1.3851864010710262E-4 1.6545282012792812E-4 22-23 0.0 0.0 0.0 3.5399208027370665E-4 2.2316892017255422E-4 24-25 0.0 0.0 0.0 9.773259607556684E-4 3.078192002380058E-4 26-27 0.0 0.0 0.0 0.0022586233817463676 3.116669402409809E-4 28-29 0.0 0.0 0.0 0.0061063633847214405 3.15514680243956E-4 30-31 0.0 0.0 0.0 0.01888855567460463 3.270579002528812E-4 32-33 0.0 0.0 0.0 0.047708128296887926 3.69383040285607E-4 34-35 0.0 0.0 0.0 0.091841706131012 3.924694803034574E-4 36-37 0.0 0.0 0.0 0.1615550595049144 4.0401270031238266E-4 38-39 0.0 0.0 0.0 0.2606305168415195 4.1170818031833274E-4 40-41 0.0 0.0 0.0 0.3625994746603619 4.23251400327258E-4 42-43 0.0 0.0 0.0 0.46740806460139994 4.347946203361832E-4 44-45 0.0 0.0 0.0 0.5951645759201812 4.4633784034510843E-4 46-47 7.695480005950145E-6 0.0 0.0 0.7498398762997762 4.886629803778342E-4 48-49 7.695480005950145E-6 0.0 0.0 0.9150233546274961 5.232926404046098E-4 50-51 7.695480005950145E-6 0.0 0.0 1.0933930379454115 5.271403804075849E-4 52-53 7.695480005950145E-6 0.0 0.0 1.2657063784986442 5.579223004313855E-4 54-55 7.695480005950145E-6 0.0 0.0 1.456500414286166 5.887042204551861E-4 56-57 7.695480005950145E-6 0.0 0.0 1.6777993328172744 5.925519604581612E-4 58-59 7.695480005950145E-6 0.0 0.0 1.9275215067503595 6.117906604730366E-4 60-61 7.695480005950145E-6 0.0 0.0 2.1828575333477858 6.233338804819618E-4 62-63 7.695480005950145E-6 0.0 0.0 2.439436379966172 6.92593200535513E-4 64-65 7.695480005950145E-6 0.0 0.0 2.703422126090286 6.964409405384881E-4 66-67 7.695480005950145E-6 0.0 0.0 2.995919625636445 7.079841605474133E-4 68-69 7.695480005950145E-6 0.0 0.0 3.321303758988032 7.233751205593137E-4 70-71 7.695480005950145E-6 0.0 0.0 3.6706823989981716 7.618525205890644E-4 72-73 7.695480005950145E-6 0.0 0.0 4.018521943007121 8.926756806902169E-4 74-75 7.695480005950145E-6 0.0 0.0 4.359612551050853 9.00371160696167E-4 76-77 7.695480005950145E-6 0.0 0.0 4.7321161084788725 9.350008207229426E-4 78-79 7.695480005950145E-6 0.0 0.0 5.137702534452472 9.465440407318679E-4 80-81 7.695480005950145E-6 0.0 0.0 5.571935384748221 0.0010273465807943444 82-83 7.695480005950145E-6 0.0 0.0 6.014744847510601 0.0011004536408508708 84-85 7.695480005950145E-6 0.0 0.0 6.442721272561512 0.0011043013808538459 86-87 7.695480005950145E-6 0.0 0.0 6.883437564762274 0.0011235400808687211 88 7.695480005950145E-6 0.0 0.0 7.235144087474214 0.0011543220008925217 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 20680 0.0 53.330807 1 GTATCAA 38015 0.0 41.60406 1 CAACGCA 47165 0.0 32.65337 5 ATCAACG 47595 0.0 32.375816 3 TATCAAC 49585 0.0 31.604578 2 TCAACGC 50275 0.0 30.752869 4 AACGCAG 50820 0.0 30.609306 6 GTACTAG 5685 0.0 26.664467 1 ACGCAGA 57745 0.0 26.581074 7 CGCAGAG 59615 0.0 25.68433 8 GTACATG 57255 0.0 25.106977 1 TACATGG 58760 0.0 24.170435 2 GCAGAGT 65635 0.0 23.077269 9 ACATGGG 59320 0.0 23.010258 3 GTGGTAT 9480 0.0 22.57446 1 GAGTACT 33065 0.0 21.207335 12-13 TAGGCAT 8180 0.0 21.046978 5 CATGGGG 40030 0.0 20.51326 4 GCATCGT 7035 0.0 20.004642 8 TAGTACT 8260 0.0 19.996082 4 >>END_MODULE