FastQCFastQC Report
Mon 6 Jun 2016
ERR1211341_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211341_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9597322
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT303790.31653621708222357No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT238970.2489965429939727No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136970.1427168954006128No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA158200.050.1917341
GTATCAA288950.041.5129621
CAACGCA347300.033.662235
ATCAACG352950.033.190283
TATCAAC370100.031.975642
TCAACGC371050.031.6848894
AACGCAG375650.031.3844556
ACGCAGA420600.027.7635387
GTACTAG39150.027.2747231
CGCAGAG430800.027.0059018
GTACATG410250.026.005231
TACATGG420550.024.7844812
GCAGAGT470000.024.0758069
ACATGGG426100.023.6767653
GAGTACT242550.021.68944512-13
GTGGTAT81150.021.2737371
CATGGGG282750.020.7566434
TACCTGG59700.020.7209242
GTACTTT255400.020.49719614-15
CAGAGTA462600.020.489410-11
Produced by FastQC (version 0.11.5)