##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211341_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9597322 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.333411237009656 31.0 31.0 34.0 28.0 34.0 2 31.353702314041353 31.0 31.0 34.0 28.0 34.0 3 31.401622244205207 31.0 31.0 34.0 28.0 34.0 4 35.010472296334335 37.0 35.0 37.0 32.0 37.0 5 35.057228360161304 37.0 35.0 37.0 32.0 37.0 6 35.12276435030522 37.0 35.0 37.0 32.0 37.0 7 35.05255580671358 37.0 35.0 37.0 32.0 37.0 8 35.05323641324111 37.0 35.0 37.0 32.0 37.0 9 36.59932729150903 39.0 37.0 39.0 32.0 39.0 10-11 36.53482841359288 39.0 37.0 39.0 32.0 39.0 12-13 36.44865041518874 39.0 37.0 39.0 32.0 39.0 14-15 37.563957633181424 40.0 37.0 41.0 32.0 41.0 16-17 37.56440171539519 40.0 37.0 41.0 32.0 41.0 18-19 37.56961019959526 40.0 37.0 41.0 32.0 41.0 20-21 37.540549071918186 40.0 37.0 41.0 31.5 41.0 22-23 37.41707639902047 40.0 37.0 41.0 31.0 41.0 24-25 37.30622271504488 40.0 37.0 41.0 31.0 41.0 26-27 37.15886160743591 40.0 36.0 41.0 30.5 41.0 28-29 37.02960357066273 39.5 36.0 41.0 30.0 41.0 30-31 36.851257621657375 39.0 36.0 41.0 30.0 41.0 32-33 36.740546164857236 39.0 35.5 41.0 30.0 41.0 34-35 36.604078929518046 39.0 35.0 41.0 30.0 41.0 36-37 36.51116290565223 39.0 35.0 41.0 30.0 41.0 38-39 36.36562152442109 39.0 35.0 40.5 29.0 41.0 40-41 36.20226006796479 39.0 35.0 40.0 28.5 41.0 42-43 36.05192844420558 39.0 35.0 40.0 28.0 41.0 44-45 35.90999937274169 38.5 35.0 40.0 27.0 41.0 46-47 35.738512368346086 38.0 34.5 40.0 27.0 41.0 48-49 35.497718999112465 38.0 34.0 40.0 26.0 41.0 50-51 34.819069840524264 37.5 33.5 39.5 25.0 40.5 52-53 35.060191686805965 38.0 33.5 40.0 26.0 40.5 54-55 35.418366811075 38.0 34.0 40.0 26.0 41.0 56-57 35.30971379307686 38.0 34.0 40.0 26.0 41.0 58-59 35.08083015241127 38.0 34.0 40.0 26.0 41.0 60-61 34.81378899238767 37.5 33.5 40.0 26.0 41.0 62-63 34.53894487441393 37.0 33.0 40.0 25.5 41.0 64-65 34.26218735809844 36.0 33.0 40.0 25.0 41.0 66-67 34.04017214385429 36.0 33.0 39.0 25.0 41.0 68-69 33.70618543381164 35.5 32.5 39.0 24.0 41.0 70-71 33.37714744800685 35.0 32.0 38.5 24.0 40.5 72-73 33.023449874871346 35.0 32.0 37.5 23.0 40.0 74-75 32.660475182556134 35.0 31.5 37.0 23.0 39.5 76-77 32.31863419816486 35.0 31.0 36.5 22.0 39.0 78-79 31.965271458017142 35.0 31.0 36.0 21.0 39.0 80-81 31.64123794116734 35.0 31.0 36.0 20.0 37.5 82-83 31.339765144901882 34.0 30.5 35.0 20.0 37.0 84-85 31.048333483027868 34.0 30.0 35.0 20.0 37.0 86-87 30.808324655565375 34.0 30.0 35.0 19.5 36.0 88-89 30.559691547287876 34.0 29.5 35.0 18.5 36.0 90-91 30.309035062072525 34.0 29.0 35.0 18.0 35.5 92-93 30.0812394853481 34.0 29.0 35.0 18.0 35.0 94-95 29.871113316819006 34.0 29.0 35.0 17.0 35.0 96-97 29.676711274249215 34.0 29.0 35.0 16.5 35.0 98-99 29.44470926368835 34.0 27.5 35.0 15.0 35.0 100 27.73185134353104 32.0 25.0 34.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.41397851930224405 1101 2 -0.46230248454994083 1101 3 -0.4709835042885935 1101 4 -0.38009417933055545 1101 5 -0.35472344548794865 1101 6 -0.40078178098425354 1101 7 -0.48671569580716323 1101 8 -0.605466862741082 1101 9 -0.6299232062158353 1101 10-11 -0.5967576693921899 1101 12-13 -0.5674582648195283 1101 14-15 -0.6078360538516847 1101 16-17 -0.6610417335877656 1101 18-19 -0.6998494686193268 1101 20-21 -0.7594559808627253 1101 22-23 -0.7242879479580182 1101 24-25 -0.7419748642997348 1101 26-27 -0.7985588059790771 1101 28-29 -0.7974718254605264 1101 30-31 -0.9271800813612074 1101 32-33 -0.9133966525403565 1101 34-35 -0.9748676003700609 1101 36-37 -1.0808665263196815 1101 38-39 -1.016559213031364 1101 40-41 -1.00055222277728 1101 42-43 -1.0422651098449691 1101 44-45 -1.1217170277259285 1101 46-47 -0.9626909052133001 1101 48-49 -0.9454200846774086 1101 50-51 -0.933232795443125 1101 52-53 -0.7974576604280088 1101 54-55 -0.7575065005000141 1101 56-57 -0.8388094484322508 1101 58-59 -0.9154336993330787 1101 60-61 -0.9616852204127042 1101 62-63 -0.8440703426706833 1101 64-65 -0.925046939923881 1101 66-67 -1.0082376441311922 1101 68-69 -1.025249686455119 1101 70-71 -1.0045064449657488 1101 72-73 -1.0637342470458364 1101 74-75 -1.0526365502577164 1101 76-77 -0.9967305488811817 1101 78-79 -1.0254380734542572 1101 80-81 -1.013676476893906 1101 82-83 -1.204934114963649 1101 84-85 -1.1256274182454682 1101 86-87 -1.1590119629008164 1101 88-89 -1.1737746137541265 1101 90-91 -1.1522805538823917 1101 92-93 -1.2089949357244159 1101 94-95 -1.2414241440707947 1101 96-97 -1.2469153228832006 1101 98-99 -1.310194825963567 1101 100 -1.3678711845139375 1102 1 -0.44103630161734486 1102 2 -0.37337117675937037 1102 3 -0.34692389857867667 1102 4 -0.2562881727937594 1102 5 -0.2861846881899197 1102 6 -0.2606600604918867 1102 7 -0.29982845677736947 1102 8 -0.3177901166119881 1102 9 -0.4000831976829531 1102 10-11 -0.395801415630487 1102 12-13 -0.3936611911396497 1102 14-15 -0.5736116442328978 1102 16-17 -0.5899419255775342 1102 18-19 -0.603475560942023 1102 20-21 -0.6267132509511768 1102 22-23 -0.647173787697362 1102 24-25 -0.6666988472746169 1102 26-27 -0.6720015366524947 1102 28-29 -0.7728416216110858 1102 30-31 -0.7090597461917767 1102 32-33 -0.7464917065184338 1102 34-35 -0.777819741335243 1102 36-37 -0.821636834442927 1102 38-39 -0.9609263388481466 1102 40-41 -0.9718534168723281 1102 42-43 -0.8861473027946758 1102 44-45 -0.9342215925618476 1102 46-47 -1.0088634522012754 1102 48-49 -0.952953486070605 1102 50-51 -0.9206567233098255 1102 52-53 -0.8465566037224619 1102 54-55 -0.763183334243486 1102 56-57 -0.8083768429331144 1102 58-59 -0.8245841319449312 1102 60-61 -0.8553858992336458 1102 62-63 -0.8517301246251705 1102 64-65 -0.821755858305373 1102 66-67 -0.7586117194186031 1102 68-69 -0.8564703578605801 1102 70-71 -0.9449882732364969 1102 72-73 -0.9787804858496045 1102 74-75 -0.9822767599227262 1102 76-77 -0.9642728069448765 1102 78-79 -0.9579748905382992 1102 80-81 -0.9682106858630775 1102 82-83 -1.0317628110839827 1102 84-85 -1.0945779059654868 1102 86-87 -0.9886642862054771 1102 88-89 -1.1914075526957966 1102 90-91 -1.2556285404024727 1102 92-93 -1.1778750398966373 1102 94-95 -1.1798870387481593 1102 96-97 -1.292547517737571 1102 98-99 -1.3159097719600972 1102 100 -1.368189694510864 1103 1 -0.34004203114360365 1103 2 -0.4614307781632512 1103 3 -0.40147916001586736 1103 4 -0.36367163217708054 1103 5 -0.32264903731675076 1103 6 -0.41029996338533437 1103 7 -0.46483633251418155 1103 8 -0.47728824178808793 1103 9 -0.6006356401473809 1103 10-11 -0.567642974658817 1103 12-13 -0.649043850198197 1103 14-15 -0.7219759988628311 1103 16-17 -0.7780941931093253 1103 18-19 -0.6996475429566757 1103 20-21 -0.8402753396961131 1103 22-23 -0.8324551424066939 1103 24-25 -0.796291144963007 1103 26-27 -0.7728408645490035 1103 28-29 -0.8184582753872718 1103 30-31 -0.8118383245696634 1103 32-33 -0.8506286249330017 1103 34-35 -0.9476355992676062 1103 36-37 -1.0634800185905249 1103 38-39 -1.0794794409106956 1103 40-41 -1.0000867602000412 1103 42-43 -1.0346518807473828 1103 44-45 -0.9861050350641634 1103 46-47 -1.031551805227565 1103 48-49 -1.0435655649398896 1103 50-51 -1.0608526660767197 1103 52-53 -0.9430136785305052 1103 54-55 -0.8425052803330999 1103 56-57 -0.8868994709120273 1103 58-59 -0.93434035321431 1103 60-61 -0.9196583078369471 1103 62-63 -0.9669926376563254 1103 64-65 -0.8212050123089583 1103 66-67 -0.8444172680135367 1103 68-69 -0.8457899093772241 1103 70-71 -0.8933042408179475 1103 72-73 -0.985618912083698 1103 74-75 -0.985511885564371 1103 76-77 -0.9382919031803283 1103 78-79 -0.9418372316826336 1103 80-81 -0.9169533763109996 1103 82-83 -1.0451734973582205 1103 84-85 -0.9749840409765547 1103 86-87 -0.9779258899338785 1103 88-89 -1.0142835429834882 1103 90-91 -0.9643193044697469 1103 92-93 -1.0396044736974446 1103 94-95 -1.1898856714900994 1103 96-97 -1.3458917607422514 1103 98-99 -1.3457652468206724 1103 100 -1.3995087059386329 1104 1 -0.349590301583536 1104 2 -0.40363091678249674 1104 3 -0.40820994784134257 1104 4 -0.30240711611629933 1104 5 -0.21823541944131364 1104 6 -0.23647629279590632 1104 7 -0.2773976624645371 1104 8 -0.2874421389518247 1104 9 -0.39946841896432517 1104 10-11 -0.3866527516854674 1104 12-13 -0.4173297985619726 1104 14-15 -0.6089335726850535 1104 16-17 -0.5306821725477704 1104 18-19 -0.6670203494758553 1104 20-21 -0.6583141106949881 1104 22-23 -0.6215485353458163 1104 24-25 -0.6766330147096227 1104 26-27 -0.6480329415080206 1104 28-29 -0.6792394803845028 1104 30-31 -0.7066403660891467 1104 32-33 -0.7374428001944651 1104 34-35 -0.7140814790665644 1104 36-37 -0.7625908368991077 1104 38-39 -0.7784772034049183 1104 40-41 -0.768370992129384 1104 42-43 -0.7972017023227593 1104 44-45 -0.8584544995372525 1104 46-47 -0.8602032775843824 1104 48-49 -0.8762422731039763 1104 50-51 -0.7553240193391773 1104 52-53 -0.5924689515359489 1104 54-55 -0.6562604412858875 1104 56-57 -0.6457381451090853 1104 58-59 -0.7370500806182037 1104 60-61 -0.6617903726667507 1104 62-63 -0.6686487438648143 1104 64-65 -0.6943728790747983 1104 66-67 -0.7171371199648391 1104 68-69 -0.668739850635113 1104 70-71 -0.7025505955276117 1104 72-73 -0.7403975831405702 1104 74-75 -0.7091106097933348 1104 76-77 -0.6721092036365945 1104 78-79 -0.7132390234647161 1104 80-81 -0.7012349487587031 1104 82-83 -0.6998073483923335 1104 84-85 -0.8005073878731537 1104 86-87 -0.7931061364639582 1104 88-89 -0.8686623140614458 1104 90-91 -0.8179669253384212 1104 92-93 -0.9155414641269992 1104 94-95 -0.9876452083589626 1104 96-97 -0.7944979187215786 1104 98-99 -0.9112804110293453 1104 100 -0.954443844615156 1105 1 -0.48071454644973244 1105 2 -0.5476866028425924 1105 3 -0.5220770945553106 1105 4 -0.43959099304820626 1105 5 -0.4152207524886151 1105 6 -0.4628835677719181 1105 7 -0.42853293288324323 1105 8 -0.4578348531236287 1105 9 -0.5871969745282613 1105 10-11 -0.5757926649075173 1105 12-13 -0.6152191986851321 1105 14-15 -0.7250001152694949 1105 16-17 -0.8087950143585658 1105 18-19 -0.837972316774021 1105 20-21 -0.8498982620490878 1105 22-23 -0.7988831456253607 1105 24-25 -0.8130863744665717 1105 26-27 -0.7628077214772517 1105 28-29 -0.8708362788722397 1105 30-31 -0.9088004627874469 1105 32-33 -0.9051113030458922 1105 34-35 -0.9335125826413275 1105 36-37 -0.9385813410439567 1105 38-39 -0.9630412025602268 1105 40-41 -0.9881074131237213 1105 42-43 -0.9995457226942719 1105 44-45 -0.980278954174743 1105 46-47 -0.9724411924394047 1105 48-49 -0.9551813093731525 1105 50-51 -0.9746907006011583 1105 52-53 -0.8056928981346374 1105 54-55 -0.7921647280783617 1105 56-57 -0.890537618778481 1105 58-59 -0.7929574499387115 1105 60-61 -0.8227609785297787 1105 62-63 -0.8747512612677042 1105 64-65 -0.811329811094275 1105 66-67 -0.8250467172793563 1105 68-69 -0.7461066951793995 1105 70-71 -0.7595427329201812 1105 72-73 -0.754798227795888 1105 74-75 -0.8048881584418268 1105 76-77 -0.8638036005229601 1105 78-79 -0.8282943137351104 1105 80-81 -0.7863098121702841 1105 82-83 -0.8420279634674728 1105 84-85 -0.8174635342089722 1105 86-87 -0.832098642191017 1105 88-89 -0.8463643245097643 1105 90-91 -0.839127397341187 1105 92-93 -0.9919301012876183 1105 94-95 -0.9654421253962404 1105 96-97 -1.0481626833176385 1105 98-99 -0.9909155080605281 1105 100 -0.9726628993650159 1106 1 -0.4851273194779395 1106 2 -0.5059863697295626 1106 3 -0.49091074520861966 1106 4 -0.3873336225901127 1106 5 -0.41181310122181713 1106 6 -0.30598921441386295 1106 7 -0.3779280915461243 1106 8 -0.44153999290561785 1106 9 -0.5567312826062434 1106 10-11 -0.5389807877710098 1106 12-13 -0.5822494114234971 1106 14-15 -0.679510525600513 1106 16-17 -0.6766940803662891 1106 18-19 -0.7163613116126868 1106 20-21 -0.729309714853791 1106 22-23 -0.6985028153852184 1106 24-25 -0.745146028000093 1106 26-27 -0.7619349749664437 1106 28-29 -0.7987403477393329 1106 30-31 -0.7813043738629304 1106 32-33 -0.7341444257963374 1106 34-35 -0.7113542436924831 1106 36-37 -0.8094846433873926 1106 38-39 -0.8712962683807035 1106 40-41 -0.8384446328673718 1106 42-43 -0.8360636241814277 1106 44-45 -0.8149764281403762 1106 46-47 -0.8172276316984224 1106 48-49 -0.8813712491918793 1106 50-51 -0.8766307183162283 1106 52-53 -0.7408367072140152 1106 54-55 -0.7913345312053721 1106 56-57 -0.6776918969844985 1106 58-59 -0.681590408721803 1106 60-61 -0.6977995864024251 1106 62-63 -0.692118882803193 1106 64-65 -0.6428819455043424 1106 66-67 -0.6989362013506266 1106 68-69 -0.794246858828636 1106 70-71 -0.8421968600976726 1106 72-73 -0.78843738413034 1106 74-75 -0.7281680105606618 1106 76-77 -0.7405009762816093 1106 78-79 -0.6836897729174183 1106 80-81 -0.8026319969187448 1106 82-83 -0.819768030956542 1106 84-85 -0.823937677124281 1106 86-87 -0.809020719360479 1106 88-89 -0.9488491099263712 1106 90-91 -0.8501143666910558 1106 92-93 -0.8238078362141152 1106 94-95 -0.9325525535285273 1106 96-97 -0.8903426106621168 1106 98-99 -0.954287945468888 1106 100 -0.9649876681469323 1107 1 -0.5372196966073268 1107 2 -0.5256923317168756 1107 3 -0.4796924761262531 1107 4 -0.3577632131804407 1107 5 -0.33581342533953773 1107 6 -0.3400112456930131 1107 7 -0.3656640841661485 1107 8 -0.35510981955969356 1107 9 -0.5206419008436356 1107 10-11 -0.4659836941284681 1107 12-13 -0.46733381384748185 1107 14-15 -0.662876485118538 1107 16-17 -0.668161957380697 1107 18-19 -0.6779370293635836 1107 20-21 -0.6465241878211145 1107 22-23 -0.6032887796710398 1107 24-25 -0.6557456953729854 1107 26-27 -0.6411609272645293 1107 28-29 -0.5987725059445737 1107 30-31 -0.666519829176913 1107 32-33 -0.6777214675375234 1107 34-35 -0.7016935071644212 1107 36-37 -0.6908164659115386 1107 38-39 -0.7079208023542805 1107 40-41 -0.6912474773769688 1107 42-43 -0.769754124649829 1107 44-45 -0.7394489453854192 1107 46-47 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0.5237289482048126 2311 10-11 0.534566912490007 2311 12-13 0.6270359752968133 2311 14-15 0.7337991662741032 2311 16-17 0.7202102961766457 2311 18-19 0.636823521982258 2311 20-21 0.6450119611611882 2311 22-23 0.7598343828220493 2311 24-25 0.7495804045221703 2311 26-27 0.8836873026139997 2311 28-29 0.8495389580330865 2311 30-31 0.8577161022260853 2311 32-33 0.8721236505711261 2311 34-35 0.911639054341272 2311 36-37 0.9002820241870566 2311 38-39 0.9226284073648756 2311 40-41 0.946843901580074 2311 42-43 0.9578290384194119 2311 44-45 0.9850072286649763 2311 46-47 1.0269846058499397 2311 48-49 0.9444728458194476 2311 50-51 0.9396672663382546 2311 52-53 0.82200281862373 2311 54-55 0.7597435537181525 2311 56-57 0.7391483762070408 2311 58-59 0.7471346201222246 2311 60-61 0.7610209134396655 2311 62-63 0.7196896831353357 2311 64-65 0.6967250375723992 2311 66-67 0.7186290557042483 2311 68-69 0.6824434988215984 2311 70-71 0.6388311346985205 2311 72-73 0.6456427815795394 2311 74-75 0.6856146539324826 2311 76-77 0.7254544708651736 2311 78-79 0.6796108322498569 2311 80-81 0.7350545262425499 2311 82-83 0.742488107015582 2311 84-85 0.7901309163180272 2311 86-87 0.7399607166068982 2311 88-89 0.784597018568288 2311 90-91 0.8427181430589776 2311 92-93 0.8659559034271176 2311 94-95 0.8859564164813385 2311 96-97 0.8947571483173817 2311 98-99 0.9301392673354307 2311 100 1.0327351842534789 2312 1 0.5215333641108302 2312 2 0.515752486958025 2312 3 0.5621317247747442 2312 4 0.4161968017693809 2312 5 0.45302583384476947 2312 6 0.4373268741778915 2312 7 0.5066856306668512 2312 8 0.5091588326345686 2312 9 0.6309331626981134 2312 10-11 0.645931713968956 2312 12-13 0.6644575984771777 2312 14-15 0.8179533028138053 2312 16-17 0.8300891235889409 2312 18-19 0.7991684851702701 2312 20-21 0.834101211308294 2312 22-23 0.810805329122104 2312 24-25 0.8369822664789694 2312 26-27 0.8549126985249487 2312 28-29 0.8381628887382533 2312 30-31 0.8685368431454847 2312 32-33 0.9038189521854676 2312 34-35 0.9337165067141342 2312 36-37 0.9332954186011051 2312 38-39 0.9548403547605986 2312 40-41 0.9475597635717818 2312 42-43 0.9434468525010757 2312 44-45 0.9288266789080524 2312 46-47 1.008563552076346 2312 48-49 1.0217651700891466 2312 50-51 1.0302323621112492 2312 52-53 0.9572702169904659 2312 54-55 0.9323685992240272 2312 56-57 0.858004752117516 2312 58-59 0.8735968483432828 2312 60-61 0.8975767332014826 2312 62-63 0.93178970747406 2312 64-65 0.8726071813236373 2312 66-67 0.8922848059914017 2312 68-69 0.9193988810191129 2312 70-71 0.8894811550179469 2312 72-73 0.9192567587495049 2312 74-75 0.8288943186127611 2312 76-77 0.8904441968754071 2312 78-79 0.8664816364786034 2312 80-81 0.871157867720072 2312 82-83 0.9042078774554057 2312 84-85 0.98832657752477 2312 86-87 0.9981167386209897 2312 88-89 1.0138396948091781 2312 90-91 1.0871324032807017 2312 92-93 1.1532697943917434 2312 94-95 1.1546885839097136 2312 96-97 1.1146490732680086 2312 98-99 1.1073549437383257 2312 100 1.2082847181107752 2313 1 0.49746824523613853 2313 2 0.48873764079182536 2313 3 0.5077942073480379 2313 4 0.4005511864514517 2313 5 0.37679598841256734 2313 6 0.43763784077429335 2313 7 0.49215349676406817 2313 8 0.5396423603409772 2313 9 0.6089652617748555 2313 10-11 0.6563010922909527 2313 12-13 0.6212356966657069 2313 14-15 0.7386816663918907 2313 16-17 0.7581351699243299 2313 18-19 0.6875702278701326 2313 20-21 0.7199036010565152 2313 22-23 0.7172377736461684 2313 24-25 0.7465563899682977 2313 26-27 0.7638607745486254 2313 28-29 0.7704799292984106 2313 30-31 0.8876194723149027 2313 32-33 0.8962623426124381 2313 34-35 0.9114255406213871 2313 36-37 0.9549271029995765 2313 38-39 0.9279672636126506 2313 40-41 0.9708310720309825 2313 42-43 0.849047190604189 2313 44-45 0.9916787703357031 2313 46-47 0.9193061880122144 2313 48-49 0.9958888657656928 2313 50-51 0.9429350553218399 2313 52-53 0.8708513853614193 2313 54-55 0.8552998253421862 2313 56-57 0.8478141320026893 2313 58-59 0.8536472909287127 2313 60-61 0.8635393201855024 2313 62-63 0.9017674036513981 2313 64-65 0.8392527670794365 2313 66-67 0.7657675973398241 2313 68-69 0.8323485653571012 2313 70-71 0.8300743017763708 2313 72-73 0.8376026888324688 2313 74-75 0.8556899671017533 2313 76-77 0.8137539908408726 2313 78-79 0.868309346396849 2313 80-81 0.9452281999722416 2313 82-83 0.8721222089458536 2313 84-85 0.8770037280492993 2313 86-87 0.927795069019588 2313 88-89 0.8743904590827327 2313 90-91 0.9643556792677224 2313 92-93 1.0680808439894953 2313 94-95 1.0541958776269382 2313 96-97 1.0432791063144222 2313 98-99 1.0816710501016722 2313 100 1.1831079707176109 2314 1 0.3854966471354899 2314 2 0.3020754032712567 2314 3 0.3611517090846519 2314 4 0.3280297147154627 2314 5 0.3071617221737952 2314 6 0.32173978149795346 2314 7 0.3988573501708075 2314 8 0.4053583873373725 2314 9 0.4625882972166835 2314 10-11 0.48507118370991265 2314 12-13 0.5356759426487443 2314 14-15 0.6168184704288038 2314 16-17 0.6372786065964959 2314 18-19 0.6137541242294375 2314 20-21 0.6737345008685409 2314 22-23 0.725524390572069 2314 24-25 0.7218355510730277 2314 26-27 0.756584123697742 2314 28-29 0.7461695253434115 2314 30-31 0.7680022785815126 2314 32-33 0.8177850314261619 2314 34-35 0.8009968205646487 2314 36-37 0.8445877654491767 2314 38-39 0.8649038523242538 2314 40-41 0.8724165568942723 2314 42-43 0.7838519660089318 2314 44-45 0.8615882893658338 2314 46-47 0.6746474007258669 2314 48-49 0.6310909160544753 2314 50-51 0.7264437182121455 2314 52-53 0.702226952852655 2314 54-55 0.6907028828757049 2314 56-57 0.6640192399573337 2314 58-59 0.7564840481651345 2314 60-61 0.5428214957785329 2314 62-63 0.650381867272813 2314 64-65 0.6804848775504624 2314 66-67 0.6951812742363757 2314 68-69 0.7060153259335848 2314 70-71 0.6508681501159472 2314 72-73 0.705161065799679 2314 74-75 0.7878504397190014 2314 76-77 0.8242620682699737 2314 78-79 0.7622787237483521 2314 80-81 0.7372540330550805 2314 82-83 0.7654602179178625 2314 84-85 0.8033265866616546 2314 86-87 0.8357518408404268 2314 88-89 0.75204312855589 2314 90-91 0.6201283945918021 2314 92-93 0.8885781485353625 2314 94-95 0.9565538106428342 2314 96-97 0.9920016544454064 2314 98-99 0.998871334577256 2314 100 1.0488532625027744 2315 1 0.30302459655084135 2315 2 0.30347327893897713 2315 3 0.3424985926700401 2315 4 0.28690043874139093 2315 5 0.3081748224334575 2315 6 0.2614910082812614 2315 7 0.2411307985958402 2315 8 0.268505168018784 2315 9 0.20717244103182963 2315 10-11 0.31949911047330914 2315 12-13 0.3439480655028646 2315 14-15 0.45670113180909055 2315 16-17 0.3197172966160764 2315 18-19 0.5251187958400223 2315 20-21 0.32259429243011795 2315 22-23 0.3182086162107183 2315 24-25 0.5363227468795628 2315 26-27 0.5644960938834842 2315 28-29 0.48236472339122116 2315 30-31 0.4697250477316288 2315 32-33 0.522872192076143 2315 34-35 0.5166176229327419 2315 36-37 0.6562702459922463 2315 38-39 0.6944055474046493 2315 40-41 0.7173579525288005 2315 42-43 0.7483465205749908 2315 44-45 0.7031285959167803 2315 46-47 0.7508579150638681 2315 48-49 0.7233202504079301 2315 50-51 0.6647924670963548 2315 52-53 0.33033312629866884 2315 54-55 0.5947727932399545 2315 56-57 0.5073315098237146 2315 58-59 0.5326506280308223 2315 60-61 0.6515006082258665 2315 62-63 0.6987852265232846 2315 64-65 0.7017832828019195 2315 66-67 0.5313536230873126 2315 68-69 0.5879902761966136 2315 70-71 0.47571176966092565 2315 72-73 0.48835620711335537 2315 74-75 0.27052861093785197 2315 76-77 0.5864267439985156 2315 78-79 0.5703442994199861 2315 80-81 0.6493082375911428 2315 82-83 0.5552091932959868 2315 84-85 0.5944146510166952 2315 86-87 0.5899037831728968 2315 88-89 0.6126424699332809 2315 90-91 0.704986517740263 2315 92-93 0.8251845038416725 2315 94-95 0.785947208964803 2315 96-97 0.829020324784107 2315 98-99 0.9692686598052731 2315 100 0.9558430802181732 2316 1 0.10125356869554736 2316 2 0.11059043827358295 2316 3 0.11086619877457338 2316 4 0.05000606931499618 2316 5 -0.05185058443812807 2316 6 -0.1485831987833066 2316 7 -0.16149981531360424 2316 8 -0.09277651490079108 2316 9 -0.03790398304685283 2316 10-11 0.036357912771869394 2316 12-13 -0.002805213058643119 2316 14-15 -0.04184406501099858 2316 16-17 0.14217888494719944 2316 18-19 0.15221998049867835 2316 20-21 0.19583231021290715 2316 22-23 0.11592980321125168 2316 24-25 0.058186457955585524 2316 26-27 -0.01146568547368787 2316 28-29 0.049687660747288476 2316 30-31 -0.48492238279669664 2316 32-33 -0.2783222131973062 2316 34-35 -0.4437895867942956 2316 36-37 -0.16458899783986425 2316 38-39 -0.06331172752815917 2316 40-41 0.13530568716982572 2316 42-43 0.18018215554331363 2316 44-45 0.002121312770164252 2316 46-47 -0.08245037470924643 2316 48-49 0.04663016837194078 2316 50-51 0.1355342674153448 2316 52-53 -0.24610304279487139 2316 54-55 -0.14799625619804857 2316 56-57 -0.15623317040692797 2316 58-59 -0.48343099231100695 2316 60-61 -0.00399871555007536 2316 62-63 0.03426467314160675 2316 64-65 -0.312901218426461 2316 66-67 -0.612370745907235 2316 68-69 -0.606335792491187 2316 70-71 -0.3764122381887063 2316 72-73 -0.5193770405781919 2316 74-75 -0.34566480912912567 2316 76-77 -0.06536262197379017 2316 78-79 -0.06154479456247941 2316 80-81 -0.09368755791862782 2316 82-83 0.11165294447716434 2316 84-85 0.12280224547705032 2316 86-87 0.19787735557852315 2316 88-89 0.17299530517824024 2316 90-91 -0.4133697312894462 2316 92-93 -0.4432520139924314 2316 94-95 -0.012142250179135061 2316 96-97 0.23520311008525852 2316 98-99 0.3291466811310748 2316 100 0.27598495186697747 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 264.0 1 684.0 2 390.0 3 391.0 4 391.0 5 356.0 6 399.0 7 847.0 8 2164.0 9 4008.0 10 6619.0 11 11087.0 12 16897.0 13 21974.0 14 26329.0 15 30655.0 16 36045.0 17 42489.0 18 50917.0 19 60888.0 20 74355.0 21 90518.0 22 100744.0 23 104285.0 24 104868.0 25 109495.0 26 120956.0 27 137728.0 28 161213.0 29 191805.0 30 232715.0 31 286119.0 32 360456.0 33 467895.0 34 642566.0 35 891381.0 36 1317654.0 37 1852686.0 38 1653756.0 39 382084.0 40 249.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.73055601587098 19.37981442166491 13.570022314007382 23.319607248456727 2 15.624061185659924 19.676348971893983 40.73741780158889 23.962172040857197 3 19.104602574994225 25.3992112277285 29.074920443299856 26.42126575397742 4 12.767430859796214 16.34757807501784 38.118646118522314 32.76634494666364 5 13.64909938569491 37.6767188844232 34.399636492296565 14.27454523758532 6 31.35666614128445 36.841437562493375 17.60165937386115 14.200236922361018 7 28.203568832168486 32.09466918490699 21.708397694888596 17.99336428803593 8 26.367933879955828 35.203084273411875 19.688447692037965 18.74053415459434 9 26.53635826759753 16.09957835003472 20.598143839609996 36.76591954275775 10-11 25.48623254680959 26.119367942379277 27.377868122781486 21.016531388029644 12-13 25.779018608641092 23.707276371622473 27.876480232813872 22.637224786922562 14-15 23.294297423986446 25.125525797084773 25.449991208269374 26.130185570659403 16-17 22.409445856805633 28.155695218298447 26.429707557584997 23.005151367310923 18-19 22.178426455450506 27.40957455374572 28.557933955809983 21.85406503499379 20-21 23.14325165232953 26.515816198377845 28.43573598775616 21.905196161536463 22-23 23.28242360987464 26.23008752876657 28.13102148584991 22.356467375508878 24-25 22.81305661434177 26.463716838518643 28.47539790024074 22.24782864689885 26-27 22.640339194887556 26.6704105465022 28.208200587719283 22.481049670890965 28-29 22.343974428179543 26.61991732233967 28.54362094125433 22.492487308226465 30-31 22.837104287259294 26.489022551500014 28.34140036581157 22.332472795429123 32-33 22.745083711198006 26.76565107163322 28.023109379692468 22.4661558374763 34-35 22.94990362292531 26.668174008076058 28.266617839646184 22.11530452935245 36-37 22.885015975360044 26.48611709315576 28.067225349100077 22.56164158238412 38-39 22.821946997561714 26.66544807123394 28.222503433879965 22.29010149732438 40-41 22.737376632206896 26.25355355644422 28.534767644921356 22.474302166427528 42-43 22.602776143315918 26.313337597992998 28.252670034190952 22.831216224500135 44-45 22.614123424952002 26.715536188643313 28.08305778784571 22.58728259855898 46-47 22.710156414043382 26.62837376144153 27.74165587075516 22.919813953759927 48-49 22.951784147617357 26.62884693879147 27.67917193823108 22.740196975360092 50-51 22.823836847780743 26.69221282607151 27.55282148542097 22.931128840726775 52-53 22.645197789122527 26.982510079110643 27.442977486267512 22.929314645499318 54-55 22.60701297939061 26.91689915541987 27.438405792002303 23.037682073187217 56-57 22.883136642624663 26.762316535207653 27.25818479491633 23.096362027251356 58-59 22.589267577367043 26.7757513137347 27.628171784523794 23.00680932437447 60-61 22.694854026763867 27.08204425339435 27.36374063437979 22.859361085461995 62-63 22.725448283965115 26.825102713551875 27.38106792902853 23.068381073454486 64-65 22.453685061493577 26.703016066055053 27.48568482421698 23.357614048234392 66-67 22.662932222528543 26.638772489400246 27.287968625331636 23.41032666273957 68-69 22.386013254036392 26.76191995346022 27.47664019091317 23.375426601590213 70-71 22.522274280686172 26.4439551365857 27.55008455156394 23.483686031164183 72-73 22.719261352475886 26.679819664877442 27.316859261285746 23.284059721360926 74-75 22.685232869480583 26.86317059775998 27.27243679691823 23.179159735841203 76-77 22.617155349325007 26.744592138369534 27.23062857285829 23.40762393944717 78-79 22.56162043693795 26.608193079193637 27.289156394117654 23.541030089750762 80-81 22.482807321106396 26.952796966479585 27.232444351374614 23.3319513610394 82-83 22.88206907846761 26.586762585758024 27.578010625451732 22.953157710322632 84-85 22.874563003226573 26.663757177631602 27.167713554479523 23.293966264662302 86-87 22.446073864352226 26.897911241404003 27.46597947527253 23.190035418971238 88-89 22.759225522479763 26.422696287207657 27.445974340685574 23.372103849627006 90-91 22.799596625841794 26.462391636727194 27.467234973163045 23.27077676426797 92-93 22.905723833223384 26.519549799634216 27.373780974111234 23.200945393031166 94-95 22.526814029470614 26.28862507033547 27.613710922363406 23.57084997783051 96-97 22.72188321185998 26.24481382966244 27.44943285149615 23.58387010698143 98-99 22.542624131262354 26.38597318169281 27.39240792775732 23.678994759287523 100 22.96073185223362 26.143686042611392 26.99383776413029 23.901744341024695 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1162.0 1 903.0 2 902.0 3 1202.0 4 1042.5 5 985.5 6 1488.0 7 2855.0 8 4806.0 9 5959.0 10 5630.5 11 5067.0 12 5486.5 13 6211.5 14 6992.5 15 7895.5 16 8673.0 17 9471.5 18 10341.0 19 11110.5 20 12033.0 21 13273.0 22 14940.0 23 17316.0 24 20050.0 25 23879.5 26 29750.5 27 36675.5 28 44125.0 29 56535.0 30 76581.5 31 96775.0 32 122310.0 33 154017.0 34 184251.0 35 212376.5 36 246366.0 37 284365.0 38 301456.0 39 305030.5 40 301042.0 41 293682.5 42 294874.0 43 305935.5 44 322051.0 45 347048.0 46 368206.5 47 377588.0 48 389485.5 49 392586.5 50 384007.0 51 378844.0 52 372519.0 53 361788.0 54 349403.0 55 322735.5 56 292759.5 57 257146.0 58 220640.5 59 195691.5 60 165907.5 61 133505.0 62 106964.5 63 84037.0 64 62240.0 65 43486.0 66 31203.0 67 23226.0 68 17469.0 69 13086.5 70 9958.5 71 7530.0 72 5756.5 73 4336.0 74 3246.5 75 2369.0 76 1621.5 77 1063.0 78 708.5 79 492.0 80 331.0 81 215.0 82 175.0 83 135.0 84 94.0 85 80.0 86 66.5 87 53.0 88 52.0 89 40.0 90 29.5 91 28.0 92 22.5 93 16.0 94 12.0 95 9.5 96 5.5 97 3.0 98 2.5 99 1.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025548793715580245 2 0.11213544778428816 3 0.017192295934219982 4 0.01782788990512145 5 0.02007851773651025 6 0.04486668260166742 7 0.04563773102538395 8 0.037718855322349296 9 0.032384033796094364 10-11 0.03627574442120417 12-13 0.035343192611438895 14-15 0.03208186617058383 16-17 0.03808875017426736 18-19 0.03697385583186643 20-21 0.04769559675084362 22-23 0.047825841417011954 24-25 0.06682072353100167 26-27 0.05905814142736901 28-29 0.05093087425846502 30-31 0.04874797365348375 32-33 0.04335584447411476 34-35 0.04452283668298302 36-37 0.03957353936858636 38-39 0.044913570681488026 40-41 0.05387961350051608 42-43 0.055327934188307955 44-45 0.05825062449712535 46-47 0.05968331582497701 48-49 0.06008967918342221 50-51 0.054947619763096416 52-53 0.06458051527290634 54-55 0.05129034953708962 56-57 0.05583849327968781 58-59 0.05321797059638095 60-61 0.049227274024983225 62-63 0.05579681498651395 64-65 0.053572236088358816 66-67 0.05207181753409962 68-69 0.05758898159299021 70-71 0.05774527519239221 72-73 0.06091282547360607 74-75 0.051160104870921284 76-77 0.05248339067919155 78-79 0.06234030701481101 80-81 0.0620746078958276 82-83 0.05283765617116942 84-85 0.059579120092042345 86-87 0.056562653623583745 88-89 0.06444506082009127 90-91 0.0700455814653296 92-93 0.07540124213817147 94-95 0.07883449153836872 96-97 0.07453120776816699 98-99 0.08311693616198351 100 0.1284420799885635 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 9597322.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.51230680710237 #Duplication Level Percentage of deduplicated Percentage of total 1 71.72180523851829 36.945556362057964 2 15.05282591278788 15.508115734668602 3 5.616764133381147 8.679974319055745 4 2.720024656261552 5.6045897846491295 5 1.4724455162947334 3.7924532596058276 6 0.8487061189870249 2.623128599419485 7 0.5663992791116501 2.0423573408644655 8 0.3807891553692495 1.5692262240158523 9 0.28524870832462335 1.322443708159289 >10 1.2139426863345666 10.752743913662496 >50 0.06965015732514543 2.466654351228948 >100 0.04522183610999622 4.681025135064019 >500 0.00435813615349888 1.5182764528862274 >1k 0.0016930536586553394 1.3018977683963378 >5k 3.135284550391989E-5 0.09375408188851256 >10k+ 9.405853651175966E-5 1.0978029643768932 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 30379 0.31653621708222357 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 23897 0.2489965429939727 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13697 0.1427168954006128 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 1.0419573293466657E-5 0.0 0.0 0.0 0.0 6 1.0419573293466657E-5 0.0 0.0 0.0 0.0 7 1.0419573293466657E-5 0.0 0.0 0.0 0.0 8 1.0419573293466657E-5 0.0 0.0 0.0 0.0 9 1.0419573293466657E-5 0.0 0.0 0.0 0.0 10-11 1.0419573293466657E-5 1.0419573293466657E-5 0.0 1.0419573293466657E-5 1.0419573293466657E-5 12-13 1.0419573293466657E-5 2.0839146586933315E-5 0.0 2.6048933233666643E-5 1.0419573293466657E-5 14-15 1.0419573293466657E-5 3.64685065271333E-5 0.0 3.64685065271333E-5 2.0839146586933315E-5 16-17 1.0419573293466657E-5 4.167829317386663E-5 0.0 4.688807982059996E-5 4.167829317386663E-5 18-19 1.0419573293466657E-5 4.167829317386663E-5 0.0 6.772722640753328E-5 4.167829317386663E-5 20-21 1.0419573293466657E-5 4.167829317386663E-5 0.0 1.0419573293466657E-4 4.167829317386663E-5 22-23 1.0419573293466657E-5 4.167829317386663E-5 0.0 2.8653826557033306E-4 5.2097866467333286E-5 24-25 1.0419573293466657E-5 5.2097866467333286E-5 0.0 6.668526907818661E-4 7.29370130542666E-5 26-27 1.0419573293466657E-5 6.251743976079994E-5 0.0 0.0015837751406069319 7.29370130542666E-5 28-29 1.0419573293466657E-5 6.772722640753328E-5 0.0 0.004407479503136396 7.29370130542666E-5 30-31 1.0419573293466657E-5 7.29370130542666E-5 0.0 0.014519675384445785 7.29370130542666E-5 32-33 1.0419573293466657E-5 7.29370130542666E-5 0.0 0.03958395894187983 8.335658634773326E-5 34-35 1.0419573293466657E-5 8.335658634773326E-5 0.0 0.07769354826273413 1.1461530622813322E-4 36-37 1.0419573293466657E-5 1.1982509287486656E-4 0.0 0.14014847058377325 1.1461530622813322E-4 38-39 1.0419573293466657E-5 1.7192295934219984E-4 0.0 0.23195533087250797 1.1461530622813322E-4 40-41 1.0419573293466657E-5 2.1360125251606646E-4 0.0 0.3312747035058321 1.1461530622813322E-4 42-43 1.0419573293466657E-5 2.500697590431998E-4 0.0 0.4320892849067688 1.1461530622813322E-4 44-45 1.0419573293466657E-5 2.500697590431998E-4 0.0 0.5543681872922468 1.1461530622813322E-4 46-47 1.5629359940199986E-5 2.500697590431998E-4 0.0 0.7001849057476659 1.250348795215999E-4 48-49 2.0839146586933315E-5 2.604893323366664E-4 0.0 0.8567389944820023 1.250348795215999E-4 50-51 2.0839146586933315E-5 2.7090890563013305E-4 0.0 1.0181017162912738 1.250348795215999E-4 52-53 2.0839146586933315E-5 2.7090890563013305E-4 0.0 1.1768126566973578 1.250348795215999E-4 54-55 2.0839146586933315E-5 2.7611869227686637E-4 0.0 1.3531118368228137 1.250348795215999E-4 56-57 2.0839146586933315E-5 2.8132847892359974E-4 0.0 1.558663968969677 1.250348795215999E-4 58-59 2.0839146586933315E-5 2.8132847892359974E-4 0.0 1.7859044429268915 1.250348795215999E-4 60-61 2.0839146586933315E-5 2.9695783886379975E-4 0.0 2.0211211002402547 1.250348795215999E-4 62-63 2.0839146586933315E-5 3.0216762551053307E-4 0.0 2.2574682812559583 1.250348795215999E-4 64-65 2.0839146586933315E-5 3.0216762551053307E-4 0.0 2.5001557726207375 1.4066423946179987E-4 66-67 2.6048933233666643E-5 3.2300677209746634E-4 0.0 2.7686942253266067 1.510838127552665E-4 68-69 3.125871988039997E-5 3.2300677209746634E-4 0.0 3.067741188635747 1.7713274598893315E-4 70-71 3.125871988039997E-5 3.3342634539093303E-4 0.0 3.389007891993204 1.8755231928239982E-4 72-73 3.125871988039997E-5 3.4384591868439967E-4 0.0 3.7120771815304314 1.8755231928239982E-4 74-75 3.125871988039997E-5 3.4384591868439967E-4 0.0 4.03064521540488 1.9276210592913316E-4 76-77 3.125871988039997E-5 3.4384591868439967E-4 0.0 4.375184035713296 2.031816792225998E-4 78-79 3.125871988039997E-5 3.4384591868439967E-4 0.0 4.749038325482879 2.0839146586933315E-4 80-81 4.167829317386663E-5 3.4384591868439967E-4 0.0 5.150061652615177 2.2402082580953313E-4 82-83 4.167829317386663E-5 3.49055705331133E-4 0.0 5.5575763739093045 2.4485997239646646E-4 84-85 4.167829317386663E-5 3.542654919778663E-4 0.0 5.955077885268411 2.500697590431998E-4 86-87 4.167829317386663E-5 3.542654919778663E-4 0.0 6.365265227112314 2.761186922768664E-4 88 4.167829317386663E-5 3.542654919778663E-4 0.0 6.689543187151583 3.0216762551053307E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 15820 0.0 50.191734 1 GTATCAA 28895 0.0 41.512962 1 CAACGCA 34730 0.0 33.66223 5 ATCAACG 35295 0.0 33.19028 3 TATCAAC 37010 0.0 31.97564 2 TCAACGC 37105 0.0 31.684889 4 AACGCAG 37565 0.0 31.384455 6 ACGCAGA 42060 0.0 27.763538 7 GTACTAG 3915 0.0 27.274723 1 CGCAGAG 43080 0.0 27.005901 8 GTACATG 41025 0.0 26.00523 1 TACATGG 42055 0.0 24.784481 2 GCAGAGT 47000 0.0 24.075806 9 ACATGGG 42610 0.0 23.676765 3 GAGTACT 24255 0.0 21.689445 12-13 GTGGTAT 8115 0.0 21.273737 1 CATGGGG 28275 0.0 20.756643 4 TACCTGG 5970 0.0 20.720924 2 GTACTTT 25540 0.0 20.497196 14-15 CAGAGTA 46260 0.0 20.4894 10-11 >>END_MODULE