FastQCFastQC Report
Mon 6 Jun 2016
ERR1211341_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211341_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9597322
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT265420.276556314355192No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT221220.23050180039806936No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130800.13628801867854387No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA161650.050.3441621
GTATCAA291650.040.857941
CAACGCA352350.032.7669875
ATCAACG356500.032.3666533
TATCAAC378400.031.1353422
TCAACGC373600.030.9228824
AACGCAG381200.030.7178546
GTACTAG41500.027.3679981
ACGCAGA426550.027.0560477
CGCAGAG439000.026.2675048
GTACATG407200.025.7715051
TACATGG418950.024.6947382
ACATGGG421550.023.7544043
GCAGAGT485250.023.5113129
GTGGTAT82800.022.276041
GAGTACT248900.021.65330512-13
TCTAACG20950.021.2763752
CATGGGG284000.021.165554
TAACGCC20200.021.1392124
TAGTACT57700.020.7378234
Produced by FastQC (version 0.11.5)