##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211341_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9597322 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.085885416786056 33.0 31.0 34.0 30.0 34.0 2 32.22210612502113 33.0 31.0 34.0 30.0 34.0 3 32.286083867979 34.0 31.0 34.0 30.0 34.0 4 35.9319767535152 37.0 35.0 37.0 35.0 37.0 5 35.832400955183125 37.0 35.0 37.0 35.0 37.0 6 35.84959095881122 37.0 35.0 37.0 35.0 37.0 7 35.76479001121354 37.0 35.0 37.0 33.0 37.0 8 35.7470300569263 37.0 35.0 37.0 33.0 37.0 9 37.35874153227327 39.0 37.0 39.0 34.0 39.0 10-11 37.30728712655468 39.0 37.0 39.0 34.0 39.0 12-13 37.238059689984354 39.0 37.0 39.0 33.0 39.0 14-15 38.46513605566219 40.0 38.0 41.0 34.0 41.0 16-17 38.44926110637947 40.0 38.0 41.0 34.0 41.0 18-19 38.458016621720105 40.0 38.0 41.0 34.0 41.0 20-21 38.42621108263326 40.0 38.0 41.0 34.0 41.0 22-23 38.317853980516645 40.0 38.0 41.0 34.0 41.0 24-25 38.22636845986828 40.0 38.0 41.0 33.0 41.0 26-27 38.12754979982958 40.0 38.0 41.0 33.0 41.0 28-29 38.033263654173524 40.0 37.0 41.0 33.0 41.0 30-31 38.04636189136928 40.0 37.0 41.0 33.0 41.0 32-33 37.989411681717044 40.0 37.0 41.0 33.0 41.0 34-35 37.8673258540247 40.0 37.0 41.0 33.0 41.0 36-37 37.72643009164432 40.0 37.0 41.0 33.0 41.0 38-39 37.618427202921815 40.0 37.0 41.0 32.5 41.0 40-41 37.51045458305973 40.0 36.5 41.0 32.0 41.0 42-43 37.404470538760705 40.0 36.0 41.0 32.0 41.0 44-45 37.243419987367304 39.5 36.0 41.0 31.0 41.0 46-47 36.99801824925745 39.0 36.0 41.0 31.0 41.0 48-49 35.4461152288107 39.0 35.0 40.5 24.0 41.0 50-51 36.169257111515066 39.0 35.0 40.0 29.0 41.0 52-53 36.260310271969615 39.0 35.0 40.0 29.5 41.0 54-55 36.08953940484648 38.0 35.0 40.0 29.0 41.0 56-57 35.86612369575596 38.0 35.0 40.0 28.5 41.0 58-59 35.61946926444689 38.0 34.0 40.0 28.0 41.0 60-61 35.36290550634854 37.5 34.0 40.0 28.0 41.0 62-63 35.03961761416362 37.0 34.0 40.0 27.5 41.0 64-65 34.6992958556564 36.0 33.5 39.0 27.0 41.0 66-67 34.359223750125295 36.0 33.0 39.0 26.0 40.0 68-69 34.00063158243518 35.5 33.0 39.0 26.0 40.0 70-71 33.64945090932658 35.0 32.5 38.0 26.0 40.0 72-73 33.27801677384588 35.0 32.0 37.0 26.0 39.5 74-75 32.916474981249976 35.0 32.0 37.0 25.0 39.0 76-77 31.990825096834303 34.0 30.5 36.0 24.5 38.0 78-79 32.43551596997579 34.5 31.5 36.0 25.0 38.0 80-81 32.31363426172426 35.0 32.0 35.5 25.0 37.0 82-83 32.051691242619555 34.0 31.0 35.0 25.0 37.0 84-85 31.77511169261592 34.0 31.0 35.0 24.5 36.5 86-87 31.52591530220618 34.0 31.0 35.0 24.0 36.0 88-89 31.301198657292105 34.0 31.0 35.0 23.5 36.0 90-91 31.086653808218585 34.0 30.5 35.0 23.0 35.0 92-93 30.896025474606354 34.0 30.0 35.0 22.0 35.0 94-95 30.703589188734107 34.0 30.0 35.0 20.0 35.0 96-97 30.51622562002192 34.0 29.5 35.0 20.0 35.0 98-99 30.29714669362974 34.0 29.0 35.0 19.5 35.0 100 27.814685179886638 31.0 25.0 34.0 15.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.33212786132145666 1101 2 -0.25952538115312507 1101 3 -0.2799111865283379 1101 4 -0.18930166410405747 1101 5 -0.15247046289794497 1101 6 -0.2526270272364215 1101 7 -0.2563177583319032 1101 8 -0.2630218689563151 1101 9 -0.3855637074528815 1101 10-11 -0.36076704141436267 1101 12-13 -0.4583687176625588 1101 14-15 -0.5509238101182135 1101 16-17 -0.5604494492648158 1101 18-19 -0.5724601335458317 1101 20-21 -0.5981995787830598 1101 22-23 -0.7099699689675063 1101 24-25 -0.6492199808868193 1101 26-27 -0.6735813850208032 1101 28-29 -0.7352252957784842 1101 30-31 -0.6301940107736854 1101 32-33 -0.5788163162884459 1101 34-35 -0.6124591425479764 1101 36-37 -0.6589210402257564 1101 38-39 -0.5652893610937326 1101 40-41 -0.5996931631624918 1101 42-43 -0.5951229071670525 1101 44-45 -0.6176466944335957 1101 46-47 -0.6382495592103794 1101 48-49 0.585316723970223 1101 50-51 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1105 68-69 -0.7879766366867287 1105 70-71 -0.8371505499447878 1105 72-73 -0.7199231109878639 1105 74-75 -0.8185659397001217 1105 76-77 -0.7971684917586259 1105 78-79 -0.6397270701949651 1105 80-81 -0.6442173385398533 1105 82-83 -0.6772881475569257 1105 84-85 -0.699763918632847 1105 86-87 -0.6729488947075666 1105 88-89 -0.7753224395792024 1105 90-91 -0.7802583436452935 1105 92-93 -0.7259796162554117 1105 94-95 -0.7063122270363031 1105 96-97 -0.7826073297250389 1105 98-99 -0.8517026098654554 1105 100 -0.7964668972004034 1106 1 -0.2928350803361326 1106 2 -0.25166781703927654 1106 3 -0.3192347902516808 1106 4 -0.19901616565324076 1106 5 -0.24196719839512326 1106 6 -0.32063792879203845 1106 7 -0.27704158148738856 1106 8 -0.3133904259769622 1106 9 -0.45011447267279436 1106 10-11 -0.3805807039527167 1106 12-13 -0.3797238768265956 1106 14-15 -0.5676587393044841 1106 16-17 -0.5661628262005891 1106 18-19 -0.6041101250733689 1106 20-21 -0.6214596134491686 1106 22-23 -0.6166145598893777 1106 24-25 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0.4449298328389659 2311 10-11 0.33168367472114824 2311 12-13 0.43879899782962895 2311 14-15 0.471940075839548 2311 16-17 0.44451183258038185 2311 18-19 0.5867110482645614 2311 20-21 0.6435884728071315 2311 22-23 0.6865099502610761 2311 24-25 0.6114822128078359 2311 26-27 0.6565725216947769 2311 28-29 0.6638529041416987 2311 30-31 0.5752236878607491 2311 32-33 0.604397664163848 2311 34-35 0.724689072626191 2311 36-37 0.686518819517751 2311 38-39 0.7145426445777616 2311 40-41 0.6744143874699873 2311 42-43 0.7308815870183878 2311 44-45 0.7802368467512579 2311 46-47 0.834456119027088 2311 48-49 -5.92353467084925 2311 50-51 -1.0639950869376946 2311 52-53 0.35309545589276325 2311 54-55 0.7561229511168577 2311 56-57 0.85288119024991 2311 58-59 0.8873696603204024 2311 60-61 0.7741770134680337 2311 62-63 0.6381303520123893 2311 64-65 0.7612428534588176 2311 66-67 0.8827721361160457 2311 68-69 0.8760096108820292 2311 70-71 0.8790991333304774 2311 72-73 0.39792153810108744 2311 74-75 0.7032899187046908 2311 76-77 0.7712368103035523 2311 78-79 0.7337556225174424 2311 80-81 0.7935937071013512 2311 82-83 0.8518456050922723 2311 84-85 0.8797752552084184 2311 86-87 0.8645144769582913 2311 88-89 0.8760108533093138 2311 90-91 0.9339526180891973 2311 92-93 0.8746859845786297 2311 94-95 0.9151358465046115 2311 96-97 0.9431801905078601 2311 98-99 0.9986707972479891 2311 100 1.181704744307389 2312 1 0.11000031321119508 2312 2 0.23660341037996346 2312 3 0.20097987904372872 2312 4 0.05513055316625781 2312 5 0.21851300656783934 2312 6 0.16960145218061484 2312 7 0.3216538488773111 2312 8 0.3452647700607301 2312 9 0.29525553372889846 2312 10-11 0.2392569769817925 2312 12-13 0.29860196404599293 2312 14-15 0.3382451087539238 2312 16-17 0.49706653158508374 2312 18-19 0.3871397085882222 2312 20-21 0.40255131536751776 2312 22-23 0.6616722446331167 2312 24-25 0.7448440594505499 2312 26-27 0.6382173712847958 2312 28-29 0.7643745771286632 2312 30-31 0.7779338698759659 2312 32-33 0.7645535677816326 2312 34-35 0.34431403747383627 2312 36-37 0.7433830794446337 2312 38-39 0.8081967914007677 2312 40-41 0.5961133418069764 2312 42-43 0.603273737950758 2312 44-45 0.7199479732054996 2312 46-47 0.8473255398212629 2312 48-49 -4.680060488711057 2312 50-51 -0.7082708964881874 2312 52-53 0.600227593804604 2312 54-55 0.9070533155806615 2312 56-57 0.9709335706517521 2312 58-59 1.0212076704653015 2312 60-61 1.0318142368858858 2312 62-63 0.7884751253102209 2312 64-65 0.9963222106814058 2312 66-67 0.9869226167847174 2312 68-69 0.9283464288567771 2312 70-71 0.9592904312665453 2312 72-73 1.0499502167942936 2312 74-75 0.7135210904306675 2312 76-77 0.9355245952908184 2312 78-79 0.6031101814819664 2312 80-81 0.785912910455707 2312 82-83 0.769720441236899 2312 84-85 0.831284155902285 2312 86-87 0.9438515754031158 2312 88-89 0.9606082612334426 2312 90-91 0.7301244571998673 2312 92-93 0.9363095948207096 2312 94-95 1.0356768766156215 2312 96-97 1.0877101002835587 2312 98-99 1.154808569808072 2312 100 0.6954033902387557 2313 1 0.06674916511008888 2313 2 0.22406505226035023 2313 3 0.27322454798625984 2313 4 0.0860726475341167 2313 5 0.19243064750020977 2313 6 0.2614584597642704 2313 7 0.19590100221739704 2313 8 0.29001145845008125 2313 9 0.4413228742267066 2313 10-11 0.23272142296863052 2313 12-13 0.04413657148161576 2313 14-15 0.19706636663325838 2313 16-17 0.108430540633627 2313 18-19 0.40678763547898455 2313 20-21 0.3462467038698378 2313 22-23 0.4587003878596718 2313 24-25 0.2169865495634511 2313 26-27 0.38005780386542654 2313 28-29 0.37599013795711045 2313 30-31 0.5617030718864271 2313 32-33 0.5166974071402066 2313 34-35 0.3982284333103365 2313 36-37 -0.3687879419082307 2313 38-39 0.11253653651461804 2313 40-41 0.31178854451597715 2313 42-43 0.42434591071889116 2313 44-45 0.40702604862867275 2313 46-47 0.3138871261905507 2313 48-49 -5.477546886975535 2313 50-51 -1.4935013012425173 2313 52-53 -0.03437643922715239 2313 54-55 0.22155672116264924 2313 56-57 0.2303945200541122 2313 58-59 0.314174693705084 2313 60-61 0.6464855738628614 2313 62-63 0.7325760581510536 2313 64-65 0.7503601778819302 2313 66-67 0.803990225026098 2313 68-69 0.7743020312625433 2313 70-71 0.8100571178013141 2313 72-73 0.5318730198968211 2313 74-75 0.6883168527885815 2313 76-77 0.709432794872388 2313 78-79 0.6276645366692861 2313 80-81 -0.26724901561691894 2313 82-83 0.13153354611817747 2313 84-85 0.4832452112145411 2313 86-87 0.6610188989637678 2313 88-89 0.6444874621397467 2313 90-91 0.7004619943346562 2313 92-93 0.7963571376228309 2313 94-95 0.7215628741153921 2313 96-97 0.7508451795677438 2313 98-99 0.8760664659904407 2313 100 1.0107354533271895 2314 1 0.05980548601495883 2314 2 0.20493084334350442 2314 3 0.2143553566291132 2314 4 0.07717970852051792 2314 5 0.2399502622703764 2314 6 0.29816925962933993 2314 7 0.15572019909166812 2314 8 0.2337962476087796 2314 9 0.38624865928485264 2314 10-11 0.3076150382651974 2314 12-13 0.21585117709268076 2314 14-15 0.2419256814136972 2314 16-17 0.4625898545703606 2314 18-19 0.5671458156491624 2314 20-21 0.618321511221005 2314 22-23 0.34421884196416386 2314 24-25 0.4947709684870034 2314 26-27 0.5530754039310679 2314 28-29 0.6681315909431262 2314 30-31 0.5334334679106476 2314 32-33 0.5148514590396331 2314 34-35 0.4041862827503593 2314 36-37 0.4959133380499239 2314 38-39 0.5321343334335253 2314 40-41 0.27356801903021477 2314 42-43 0.3135940124366101 2314 44-45 0.5562299686214871 2314 46-47 0.6051218091175699 2314 48-49 -6.406360643973898 2314 50-51 -1.5544902790543773 2314 52-53 -0.760220639050246 2314 54-55 -0.3401980131076101 2314 56-57 -0.027883601976917305 2314 58-59 0.3887483649571237 2314 60-61 0.625536335946741 2314 62-63 0.47826970064451757 2314 64-65 0.6446765721026253 2314 66-67 0.7267592563615892 2314 68-69 0.7145377575644787 2314 70-71 0.5040428976616127 2314 72-73 0.6754609789499355 2314 74-75 0.7506768255718299 2314 76-77 0.7329174156386138 2314 78-79 0.47706345048077026 2314 80-81 0.5613243118689724 2314 82-83 0.5565303987885741 2314 84-85 0.5695887950543472 2314 86-87 0.6610775857009941 2314 88-89 0.5280487652549652 2314 90-91 0.6997838833544385 2314 92-93 0.7850436333207078 2314 94-95 0.8778279459365415 2314 96-97 0.8813913574688748 2314 98-99 0.9223531050841274 2314 100 -0.509782736914449 2315 1 0.16674665477712125 2315 2 0.23788038265052336 2315 3 0.32979936435868495 2315 4 0.14690077192653206 2315 5 0.2662968342830965 2315 6 0.29924510738521803 2315 7 0.3232735088865937 2315 8 0.2820715541807246 2315 9 0.4531985540060077 2315 10-11 0.19212682025015226 2315 12-13 0.4215030812442677 2315 14-15 0.2434080649539183 2315 16-17 0.3712016381364336 2315 18-19 0.49013557140281705 2315 20-21 0.42852888039087844 2315 22-23 0.488530330028496 2315 24-25 0.5353443766914197 2315 26-27 0.599776707279787 2315 28-29 0.6750606912755188 2315 30-31 0.6208303390411416 2315 32-33 0.7176414728197074 2315 34-35 0.6938377132730125 2315 36-37 0.6187267529739202 2315 38-39 0.7010970886172387 2315 40-41 0.7512885928357846 2315 42-43 0.7520544656547372 2315 44-45 0.7797607479441027 2315 46-47 0.6865231513719792 2315 48-49 -4.957158438379359 2315 50-51 -0.9377418754754387 2315 52-53 0.2431477292906763 2315 54-55 0.2503636698299516 2315 56-57 0.5723926778306705 2315 58-59 0.6799159581155081 2315 60-61 0.5629395267266872 2315 62-63 0.3999256912568683 2315 64-65 0.5763472186571192 2315 66-67 -0.18770284525714942 2315 68-69 0.5882335260805149 2315 70-71 0.533990419074577 2315 72-73 0.7179658490568457 2315 74-75 0.8264262668817892 2315 76-77 0.7799446435619863 2315 78-79 0.7480190055295921 2315 80-81 0.5917859762917743 2315 82-83 0.5195322348383655 2315 84-85 0.566261405815542 2315 86-87 0.6226557607587822 2315 88-89 0.6139329935747746 2315 90-91 0.7090346009577679 2315 92-93 0.789077721647697 2315 94-95 0.4281453363385701 2315 96-97 0.5195574394597244 2315 98-99 0.4289557115763145 2315 100 0.37371499870753055 2316 1 0.27060939267715867 2316 2 0.2877698626632892 2316 3 0.22283460407492583 2316 4 0.002233878358019581 2316 5 0.17070117931443463 2316 6 0.11856938467959566 2316 7 0.25537361104035483 2316 8 0.30912928766327497 2316 9 0.4735847549420171 2316 10-11 0.41581557658312107 2316 12-13 0.33171813137428074 2316 14-15 0.4414771513413527 2316 16-17 0.4530178349883087 2316 18-19 0.16533382413398812 2316 20-21 0.44910837207802246 2316 22-23 0.5813377500351677 2316 24-25 0.6295544517564409 2316 26-27 0.6476507918058516 2316 28-29 0.7416399786838994 2316 30-31 0.6402720622451525 2316 32-33 0.5792845978208163 2316 34-35 0.5456295764117982 2316 36-37 0.4647364055528769 2316 38-39 0.5516272835539127 2316 40-41 0.15576716985542305 2316 42-43 0.11833007413297025 2316 44-45 0.3866116259329786 2316 46-47 0.369649344505909 2316 48-49 -5.111185692099667 2316 50-51 -1.0505259203742057 2316 52-53 0.22147129371831653 2316 54-55 0.5023377973638432 2316 56-57 0.41333810314389297 2316 58-59 0.317243986144355 2316 60-61 0.4544028696407949 2316 62-63 0.40163109779484785 2316 64-65 0.47357312072495716 2316 66-67 0.5133695271808989 2316 68-69 0.23084512222188636 2316 70-71 -0.1457949870914632 2316 72-73 -0.0024461072037240683 2316 74-75 0.19636758155223077 2316 76-77 0.1574627855244195 2316 78-79 0.3108459767681353 2316 80-81 0.2285888204925044 2316 82-83 0.1312947660966799 2316 84-85 0.26918125007863125 2316 86-87 0.2942398802562032 2316 88-89 0.1465568560279671 2316 90-91 0.15062569718365637 2316 92-93 0.320536243736381 2316 94-95 0.564421786472149 2316 96-97 0.6700757069515006 2316 98-99 0.6371701119637407 2316 100 -0.23119422449778781 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 3.0 11 17.0 12 46.0 13 196.0 14 639.0 15 2037.0 16 4660.0 17 9774.0 18 17120.0 19 25859.0 20 38679.0 21 55663.0 22 70434.0 23 75403.0 24 78193.0 25 86803.0 26 103999.0 27 127948.0 28 157784.0 29 194660.0 30 239507.0 31 297110.0 32 374980.0 33 489061.0 34 676900.0 35 953412.0 36 1484942.0 37 2150179.0 38 1669970.0 39 211322.0 40 21.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.72943478416304 20.188013037966464 14.400369964232413 23.682182213638082 2 15.306516805369434 20.086252770486542 40.360030138142186 24.247200286001842 3 18.68251761950211 25.40369603180888 29.683849241246513 26.229937107442495 4 12.749541390151139 16.720609731720796 38.02108573549848 32.50876314262959 5 13.503708083805039 37.79241503447594 34.53755362850393 14.166323253215104 6 31.457739699543115 37.15090373695065 17.312974032221714 14.078382531284525 7 28.129576146345826 32.15090626322635 21.640974430158746 18.078543160269085 8 26.41607951856391 35.557282029277204 19.434114199151107 18.592524253007777 9 26.311861450857844 16.020369798394476 20.513967581888306 37.153801168859374 10-11 25.442645317279787 26.283498583521443 27.31640234607632 20.957453753122454 12-13 25.65649871865204 23.69784153172007 27.950661200554528 22.69499854907336 14-15 23.103323097133867 25.211991649328482 25.43243358140279 26.252251672134864 16-17 22.322138732801722 28.2755221382535 26.394194278062127 23.008144850882655 18-19 22.076238775383256 27.460923730695264 28.587967412989524 21.87487008093196 20-21 23.045602224038237 26.544429031572054 28.459417355913114 21.95055138847659 22-23 23.121036058381854 26.277304953676015 28.200242214031725 22.401416773910405 24-25 22.720952307519184 26.476969612350114 28.515892978456485 22.286185101674217 26-27 22.532025884322852 26.7240577182264 28.232979744855726 22.510936652595024 28-29 22.295915387638292 26.67330504286837 28.55915195916237 22.471627610330962 30-31 22.765760755796478 26.504006791231628 28.401177399669752 22.329055053302145 32-33 22.734855111382323 26.75921068783459 28.05788958908158 22.448044611701505 34-35 22.85137068955949 26.657190749861382 28.366253181747147 22.125185378831976 36-37 22.813265022182712 26.4779199421384 28.15921528329986 22.549599752379027 38-39 22.728536840940766 26.63196494240694 28.343235667600265 22.296262549052027 40-41 22.621154023796 26.26070212828367 28.652790710966062 22.465353136954263 42-43 22.509630015868805 26.288653831786103 28.349477748716055 22.852238403629034 44-45 22.531444321603836 26.686998150825936 28.155989823941603 22.62556770362863 46-47 22.62333838846933 26.606058180853402 27.834484364144448 22.93611906653282 48-49 22.956216111210598 26.711669270515298 27.684637546168485 22.64747707210562 50-51 22.74971017857835 26.676555958565856 27.565039428635984 23.008694434219812 52-53 22.558052902646438 26.93740035361391 27.51533310923508 22.989213634504573 54-55 22.553045423918434 26.860557471668017 27.48698001062482 23.09941709378873 56-57 22.82858858798777 26.731386709419468 27.290284694099093 23.149740008493666 58-59 22.457615263189236 26.783748265583384 27.682490117648296 23.07614635357908 60-61 22.61749929989775 27.065502217533165 27.4046148135123 22.912383669056783 62-63 22.642147828698388 26.82985234136195 27.370997460140543 23.157002369799116 64-65 22.314366154322343 26.711498749144774 27.47945767961536 23.494677416917522 66-67 22.526964768030634 26.66104748126546 27.303633676777988 23.50835407392592 68-69 22.283714119636805 26.74865227319558 27.40648824549176 23.56114536167585 70-71 22.38080545096027 26.450468699850692 27.491440273319267 23.67728557586977 72-73 22.57723379937646 26.68060646349602 27.257986275348365 23.48417346177915 74-75 22.53727233389717 26.869525143601614 27.160029083739758 23.433173438761457 76-77 22.43314740371564 26.735609125748688 27.139572266352648 23.691671204183027 78-79 22.35132245006672 26.598857959344784 27.171623713880866 23.87819587670763 80-81 22.279562672523458 26.964760943307198 27.073565046895332 23.682111337274016 82-83 22.642745410527862 26.558853200303687 27.449045855929004 23.349355533239443 84-85 22.64434336048864 26.649296151238023 26.98219368442577 23.724166803847567 86-87 22.183803855981587 26.883638693425834 27.300184964345053 23.632372486247526 88-89 22.40336591049931 26.4359295613613 27.254320307973746 23.906384220165645 90-91 22.465144165758495 26.424334015715388 27.285400605650118 23.825121212876 92-93 22.51518227905298 26.514856728639348 27.15145680683814 23.81850418546953 94-95 22.0974224753646 26.2619757870082 27.403711116547 24.2368906210802 96-97 22.25080188174221 26.240128707457544 27.292634149937623 24.216435260862625 98-99 21.992071369141012 26.367084916655692 27.26221610661936 24.37862760758394 100 22.11062410127507 26.19231014572633 27.107659794282927 24.58940595871567 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 998.0 1 742.0 2 628.0 3 831.5 4 755.0 5 721.5 6 1193.0 7 2421.0 8 4068.5 9 5108.0 10 4908.0 11 4485.5 12 4819.0 13 5604.0 14 6508.5 15 7421.0 16 8267.0 17 9218.0 18 10327.5 19 11172.5 20 12116.0 21 13442.5 22 15086.0 23 17540.0 24 20348.5 25 24037.5 26 29835.5 27 36812.5 28 44277.0 29 56825.0 30 76971.0 31 97852.5 32 123512.5 33 154607.5 34 184606.0 35 212834.0 36 246866.0 37 285240.5 38 301998.5 39 303046.5 40 297770.0 41 290468.0 42 291868.0 43 303306.5 44 319990.0 45 346250.0 46 368909.0 47 379614.5 48 391501.0 49 395599.5 50 388741.5 51 381735.5 52 375756.5 53 367391.0 54 355227.5 55 328019.5 56 296929.0 57 260971.0 58 223679.0 59 197373.5 60 166520.5 61 132538.5 62 104103.5 63 79824.5 64 57994.5 65 39986.5 66 28370.0 67 21046.5 68 15604.0 69 11533.0 70 8805.0 71 6563.5 72 4897.0 73 3634.0 74 2635.0 75 1818.0 76 1144.0 77 645.5 78 378.0 79 219.5 80 140.5 81 82.0 82 54.5 83 39.5 84 26.5 85 18.0 86 11.5 87 9.5 88 8.0 89 5.5 90 4.0 91 3.5 92 1.5 93 0.0 94 0.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.030018790658477436 2 0.0018859427661174649 3 2.083914658693331E-5 4 0.021047538052802645 5 4.897199447929329E-4 6 5.209786646733328E-5 7 0.0 8 0.0038344029719957293 9 0.009981951215141057 10-11 0.0011096845557541988 12-13 7.29370130542666E-5 14-15 1.0419573293466656E-4 16-17 3.282165587441997E-4 18-19 1.0419573293466656E-5 20-21 5.209786646733328E-5 22-23 2.604893323366664E-4 24-25 2.083914658693331E-5 26-27 7.29370130542666E-5 28-29 9.950692495260657E-4 30-31 0.002511117163725464 32-33 0.009544329136815457 34-35 0.018427015369495783 36-37 0.020964181466454915 38-39 0.02110484570591671 40-41 0.004766954781760996 42-43 0.005261884513200661 44-45 0.009122336418430058 46-47 0.011888733127845455 48-49 1.2003765217005327 50-51 0.018317609849914384 52-53 0.01905218976710378 54-55 0.009627685723163191 56-57 0.007903246343094458 58-59 0.008288770554952725 60-61 0.005308772593021262 62-63 0.007585449357643726 64-65 0.019661734804771583 66-67 0.02929463031458151 68-69 0.02951865114039104 70-71 0.02641882808558471 72-73 0.023459669270240176 74-75 0.009320308311005925 76-77 0.01455614389097292 78-79 0.008502371807468791 80-81 0.0026830401230676644 82-83 0.01422792733222872 84-85 0.024251556840543642 86-87 0.023230438657783913 88-89 0.03258721547531697 90-91 0.020974601039748377 92-93 0.013191179789528787 94-95 0.006402827788835261 96-97 0.0037979344654685966 98-99 0.015275094448222119 100 0.03307172563346317 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 9597322.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.50882822640784 #Duplication Level Percentage of deduplicated Percentage of total 1 71.46131669564998 36.80888686509166 2 15.303642231556402 15.76545357887279 3 5.622478098074466 8.688217756813737 4 2.6994947641704226 5.5619124842296666 5 1.4755916349935652 3.800299802960392 6 0.8824100974385561 2.7271146080526236 7 0.5616587399745067 2.025126849144527 8 0.38562328144545494 1.5890402691262129 9 0.26430607042556437 1.2252686382672522 >10 1.2223945210743659 10.793016860061662 >50 0.06881871171719976 2.4332613986633285 >100 0.04620000861120898 4.740099239692623 >500 0.004296169153836751 1.4994823282457976 >1k 0.001642620306324564 1.2460368202157135 >5k 3.158885201996462E-5 0.09214294213779979 >10k+ 9.476655605989387E-5 1.0046395584242116 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 26542 0.276556314355192 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 22122 0.23050180039806936 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13080 0.13628801867854387 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 1.0419573293466657E-5 0.0 1.0419573293466657E-5 0.0 4 0.0 1.0419573293466657E-5 0.0 1.0419573293466657E-5 0.0 5 0.0 1.0419573293466657E-5 0.0 1.0419573293466657E-5 0.0 6 0.0 1.0419573293466657E-5 0.0 1.0419573293466657E-5 3.125871988039997E-5 7 0.0 1.0419573293466657E-5 0.0 1.0419573293466657E-5 3.125871988039997E-5 8 0.0 1.0419573293466657E-5 0.0 1.0419573293466657E-5 3.125871988039997E-5 9 0.0 1.0419573293466657E-5 0.0 1.0419573293466657E-5 3.125871988039997E-5 10-11 0.0 1.0419573293466657E-5 0.0 1.0419573293466657E-5 3.125871988039997E-5 12-13 0.0 1.0419573293466657E-5 0.0 1.5629359940199986E-5 3.125871988039997E-5 14-15 0.0 3.125871988039997E-5 0.0 2.0839146586933315E-5 6.772722640753328E-5 16-17 0.0 4.167829317386663E-5 0.0 2.6048933233666643E-5 8.335658634773326E-5 18-19 0.0 4.167829317386663E-5 0.0 4.688807982059996E-5 8.335658634773326E-5 20-21 0.0 4.167829317386663E-5 0.0 8.856637299446658E-5 8.335658634773326E-5 22-23 0.0 4.167829317386663E-5 0.0 2.6569911898339973E-4 9.377615964119992E-5 24-25 0.0 6.772722640753328E-5 0.0 6.408037575481994E-4 1.6671317269546652E-4 26-27 0.0 8.335658634773326E-5 0.0 0.0015785653539601985 1.6671317269546652E-4 28-29 0.0 8.856637299446658E-5 0.0 0.004417899076429862 1.6671317269546652E-4 30-31 0.0 9.377615964119991E-5 0.0 0.014587402610853319 1.7713274598893315E-4 32-33 0.0 9.377615964119991E-5 0.0 0.039776721047808966 1.7713274598893315E-4 34-35 0.0 9.377615964119991E-5 0.0 0.07795924738171753 1.8755231928239982E-4 36-37 0.0 1.094055195813999E-4 0.0 0.13982025402502907 1.8755231928239982E-4 38-39 0.0 1.510838127552665E-4 0.0 0.22798026366105045 1.8755231928239982E-4 40-41 0.0 1.8755231928239982E-4 0.0 0.3225743598057875 1.8755231928239982E-4 42-43 0.0 2.917480522170664E-4 0.0 0.41886163661071285 1.9797189257586648E-4 44-45 0.0 2.917480522170664E-4 0.0 0.5356129553640068 1.9797189257586648E-4 46-47 0.0 2.917480522170664E-4 0.0 0.6752456570697534 2.552795456899331E-4 48-49 0.0 3.125871988039997E-4 0.0 0.8262669523852592 2.604893323366664E-4 50-51 0.0 3.4384591868439967E-4 0.0 0.9874525414485416 2.7611869227686637E-4 52-53 0.0 3.542654919778663E-4 0.0 1.1459498806021096 2.917480522170664E-4 54-55 0.0 3.64685065271333E-4 0.0 1.322176123714511 3.0216762551053307E-4 56-57 1.0419573293466657E-5 3.64685065271333E-4 0.0 1.5278064026610756 3.125871988039997E-4 58-59 1.0419573293466657E-5 3.64685065271333E-4 0.0 1.7551302332046377 3.3342634539093303E-4 60-61 1.0419573293466657E-5 3.7510463856479964E-4 0.0 1.9902947926515333 3.4384591868439967E-4 62-63 1.0419573293466657E-5 3.7510463856479964E-4 0.0 2.226266869028673 3.542654919778663E-4 64-65 1.0419573293466657E-5 3.7510463856479964E-4 0.0 2.4682302000495557 3.64685065271333E-4 66-67 1.0419573293466657E-5 3.9594378515173296E-4 0.0 2.7365758906494957 3.64685065271333E-4 68-69 1.0419573293466657E-5 3.9594378515173296E-4 0.0 3.0356801616117495 3.64685065271333E-4 70-71 1.0419573293466657E-5 4.063633584451996E-4 0.0 3.3571760955816634 3.9594378515173296E-4 72-73 1.0419573293466657E-5 4.167829317386663E-4 0.0 3.6808080420767375 4.6367101155926627E-4 74-75 1.0419573293466657E-5 4.167829317386663E-4 0.0 4.000204432028018 4.793003714994662E-4 76-77 1.0419573293466657E-5 4.3762207832559957E-4 0.0 4.345748741159253 4.8451015814619954E-4 78-79 2.0839146586933315E-5 4.3762207832559957E-4 0.0 4.719993764927342 4.897199447929329E-4 80-81 2.0839146586933315E-5 4.428318649723329E-4 0.0 5.121835028563177 4.949297314396663E-4 82-83 2.0839146586933315E-5 4.4804165161906626E-4 0.0 5.5296206587629335 5.209786646733328E-4 84-85 2.0839146586933315E-5 4.4804165161906626E-4 0.0 5.928591329956419 5.209786646733328E-4 86-87 2.0839146586933315E-5 4.4804165161906626E-4 0.0 6.340018601022243 5.418178112602662E-4 88 2.0839146586933315E-5 4.4804165161906626E-4 0.0 6.664973833325588 5.626569578471995E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 16165 0.0 50.344162 1 GTATCAA 29165 0.0 40.85794 1 CAACGCA 35235 0.0 32.766987 5 ATCAACG 35650 0.0 32.366653 3 TATCAAC 37840 0.0 31.135342 2 TCAACGC 37360 0.0 30.922882 4 AACGCAG 38120 0.0 30.717854 6 GTACTAG 4150 0.0 27.367998 1 ACGCAGA 42655 0.0 27.056047 7 CGCAGAG 43900 0.0 26.267504 8 GTACATG 40720 0.0 25.771505 1 TACATGG 41895 0.0 24.694738 2 ACATGGG 42155 0.0 23.754404 3 GCAGAGT 48525 0.0 23.511312 9 GTGGTAT 8280 0.0 22.27604 1 GAGTACT 24890 0.0 21.653305 12-13 TCTAACG 2095 0.0 21.276375 2 CATGGGG 28400 0.0 21.16555 4 TAACGCC 2020 0.0 21.139212 4 TAGTACT 5770 0.0 20.737823 4 >>END_MODULE