FastQCFastQC Report
Mon 6 Jun 2016
ERR1211340_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211340_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7831004
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT268490.3428551434784097No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT212000.27071879927529086No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122270.15613579050655574No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA138350.051.706011
GTATCAA250700.042.548051
CAACGCA312700.033.136395
ATCAACG313650.033.0212553
TATCAAC323100.032.5749322
TCAACGC331850.031.3803674
AACGCAG335250.031.3002096
GTACTAG31050.028.4765381
ACGCAGA373450.027.6591727
GTACATG373400.027.2441431
CGCAGAG384850.026.827478
TACATGG380700.026.5716152
GTGGTAT59750.025.345971
ACATGGG391100.025.1723353
GCAGAGT416750.024.2668489
CATGGGG275350.023.5517984
TACCTGG52900.022.6696572
ATGGGGG188100.022.3843125
GAGTACT210500.022.11243612-13
TAGTACT45950.021.8855934
Produced by FastQC (version 0.11.5)