##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211340_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7831004 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.44831837654533 31.0 31.0 34.0 28.0 34.0 2 31.46713192842195 31.0 31.0 34.0 28.0 34.0 3 31.518157824973656 33.0 31.0 34.0 28.0 34.0 4 35.12735263575399 37.0 35.0 37.0 32.0 37.0 5 35.171251987612315 37.0 35.0 37.0 32.0 37.0 6 35.236629556056926 37.0 35.0 37.0 32.0 37.0 7 35.16748031286921 37.0 35.0 37.0 32.0 37.0 8 35.16664823565407 37.0 35.0 37.0 32.0 37.0 9 36.72658486702343 39.0 37.0 39.0 32.0 39.0 10-11 36.65730396766494 39.0 37.0 39.0 32.0 39.0 12-13 36.5698363836872 39.0 37.0 39.0 32.0 39.0 14-15 37.708845506910734 40.0 37.0 41.0 32.0 41.0 16-17 37.710089536411935 40.0 37.0 41.0 32.0 41.0 18-19 37.70862280238907 40.0 37.0 41.0 32.0 41.0 20-21 37.67180402410725 40.0 37.0 41.0 32.0 41.0 22-23 37.55173442639028 40.0 37.0 41.0 31.5 41.0 24-25 37.44055940975129 40.0 37.0 41.0 31.0 41.0 26-27 37.296111967252216 40.0 36.5 41.0 31.0 41.0 28-29 37.1615675972072 40.0 36.0 41.0 31.0 41.0 30-31 36.97180904262085 39.0 36.0 41.0 30.0 41.0 32-33 36.85120675458728 39.0 36.0 41.0 30.0 41.0 34-35 36.713853740848556 39.0 35.0 41.0 30.0 41.0 36-37 36.62566702813586 39.0 35.0 41.0 30.0 41.0 38-39 36.476966810386 39.0 35.0 41.0 29.5 41.0 40-41 36.305244576557484 39.0 35.0 40.0 29.0 41.0 42-43 36.15099066479854 39.0 35.0 40.0 28.0 41.0 44-45 36.00729255150425 39.0 35.0 40.0 28.0 41.0 46-47 35.8308224590359 38.5 35.0 40.0 27.0 41.0 48-49 35.58520178255559 38.0 34.0 40.0 26.0 41.0 50-51 34.89817308227655 37.5 33.5 39.5 25.5 40.5 52-53 35.130583447537504 38.0 34.0 40.0 26.0 40.5 54-55 35.47748328311414 38.0 34.0 40.0 26.0 41.0 56-57 35.36491074452267 38.0 34.0 40.0 26.0 41.0 58-59 35.13230493816629 38.0 34.0 40.0 26.0 41.0 60-61 34.85816358924092 37.0 34.0 40.0 26.0 41.0 62-63 34.572472124902504 37.0 33.0 40.0 26.0 41.0 64-65 34.28657538675756 36.0 33.0 39.5 25.0 41.0 66-67 34.062179958023265 36.0 33.0 39.0 25.0 41.0 68-69 33.72855262237128 35.5 33.0 39.0 24.5 41.0 70-71 33.39479337515343 35.0 32.0 38.5 24.0 40.0 72-73 33.03755195374693 35.0 32.0 37.5 23.5 40.0 74-75 32.67176973731593 35.0 32.0 37.0 23.0 39.0 76-77 32.32975426905669 35.0 31.0 36.5 22.5 39.0 78-79 31.980806931014207 35.0 31.0 36.0 21.5 39.0 80-81 31.667101178852676 35.0 31.0 35.5 20.5 37.0 82-83 31.370602096487247 34.0 31.0 35.0 20.0 37.0 84-85 31.083483995155667 34.0 30.0 35.0 20.0 36.5 86-87 30.8423068740611 34.0 30.0 35.0 19.5 36.0 88-89 30.601465342119603 34.0 30.0 35.0 19.0 36.0 90-91 30.356000967436614 34.0 29.0 35.0 18.0 35.5 92-93 30.127082938535086 34.0 29.0 35.0 18.0 35.0 94-95 29.91365992151198 34.0 29.0 35.0 17.0 35.0 96-97 29.714942745527907 34.0 29.0 35.0 16.5 35.0 98-99 29.487322889887427 34.0 28.5 35.0 15.0 35.0 100 27.767097169149704 32.0 25.0 34.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.3724338089423149 1101 2 -0.4889337415024073 1101 3 -0.465736977148989 1101 4 -0.38360149132267196 1101 5 -0.4061793991595266 1101 6 -0.39882771385961036 1101 7 -0.49481974516942984 1101 8 -0.548608240757126 1101 9 -0.5189519211799052 1101 10-11 -0.5468364626047162 1101 12-13 -0.5164286372656264 1101 14-15 -0.631959677288016 1101 16-17 -0.6189267588029352 1101 18-19 -0.750572136574732 1101 20-21 -0.7621097811833337 1101 22-23 -0.7469765618392614 1101 24-25 -0.7560612256686952 1101 26-27 -0.8252697599219303 1101 28-29 -0.924550758500402 1101 30-31 -0.9194152615191982 1101 32-33 -0.9366759086619467 1101 34-35 -0.8840193356507555 1101 36-37 -0.9791143773842421 1101 38-39 -1.0056122186069132 1101 40-41 -0.9932334738094468 1101 42-43 -1.0739270182714264 1101 44-45 -1.1324471002546872 1101 46-47 -1.0834591332759587 1101 48-49 -1.1042126519436337 1101 50-51 -1.0583678074198488 1101 52-53 -0.8262041548448522 1101 54-55 -0.8320051449988384 1101 56-57 -0.7914865472676809 1101 58-59 -0.7964214082891985 1101 60-61 -0.8101974777493268 1101 62-63 -0.7615202836076591 1101 64-65 -0.8045969500115504 1101 66-67 -0.8689649607172356 1101 68-69 -0.897378606862631 1101 70-71 -0.90237568943882 1101 72-73 -0.9325180755535882 1101 74-75 -0.9925624876128687 1101 76-77 -1.0384120135961865 1101 78-79 -1.023297496690411 1101 80-81 -1.1778198812510077 1101 82-83 -1.2593188634642658 1101 84-85 -1.1344859465748236 1101 86-87 -1.1717708853240332 1101 88-89 -1.2721384765141757 1101 90-91 -1.2315659140231823 1101 92-93 -1.2905459054587034 1101 94-95 -1.2835044885052227 1101 96-97 -1.4040229842724266 1101 98-99 -1.3972644931766034 1101 100 -1.417534073809275 1102 1 -0.4464028954164263 1102 2 -0.4949333981260722 1102 3 -0.3934278195615768 1102 4 -0.29204877551154595 1102 5 -0.3811600729254536 1102 6 -0.3882186523451594 1102 7 -0.39126122877244995 1102 8 -0.39448718158897833 1102 9 -0.4873050243002268 1102 10-11 -0.45244693172075756 1102 12-13 -0.5081698404345829 1102 14-15 -0.6331849544935082 1102 16-17 -0.6881230876934907 1102 18-19 -0.6230466005998778 1102 20-21 -0.6961245339946132 1102 22-23 -0.7336908697096405 1102 24-25 -0.7758874751300979 1102 26-27 -0.6830194741772928 1102 28-29 -0.7488376868451923 1102 30-31 -0.7630283107855504 1102 32-33 -0.8111373841301557 1102 34-35 -0.8530897422831671 1102 36-37 -0.8847426489641492 1102 38-39 -0.9534922997016579 1102 40-41 -1.0558720840948013 1102 42-43 -1.0302381124048168 1102 44-45 -0.9990670807142195 1102 46-47 -1.013919956087733 1102 48-49 -0.9487436218942804 1102 50-51 -0.9232561588151427 1102 52-53 -0.8569956282491731 1102 54-55 -0.8407457021495688 1102 56-57 -0.793007262395669 1102 58-59 -0.7371655678741007 1102 60-61 -0.9110569973000509 1102 62-63 -0.8912154571484834 1102 64-65 -1.0223132755950814 1102 66-67 -0.9524951017972327 1102 68-69 -0.9169014600967031 1102 70-71 -0.8836586239969648 1102 72-73 -0.94702542404859 1102 74-75 -0.9313125449428377 1102 76-77 -0.9512718572121059 1102 78-79 -0.9110328211237189 1102 80-81 -0.9722701064672634 1102 82-83 -1.0611478704971447 1102 84-85 -1.1435397063948436 1102 86-87 -1.1311029670536392 1102 88-89 -1.1383515273495632 1102 90-91 -1.2004601064370384 1102 92-93 -1.1333257466124458 1102 94-95 -1.2610080203330156 1102 96-97 -1.3618091935787113 1102 98-99 -1.417125102901938 1102 100 -1.4529467198347383 1103 1 -0.40674472761476466 1103 2 -0.4645655529151327 1103 3 -0.41563334594823687 1103 4 -0.31403155190741927 1103 5 -0.2970548858810673 1103 6 -0.36092537402138447 1103 7 -0.35594836500734317 1103 8 -0.3947285201133539 1103 9 -0.5535080866908402 1103 10-11 -0.5379375307651486 1103 12-13 -0.6267936414741655 1103 14-15 -0.6418958303164786 1103 16-17 -0.6503588610608446 1103 18-19 -0.6504129834086996 1103 20-21 -0.7967073821515953 1103 22-23 -0.7471291688994341 1103 24-25 -0.7058646838696419 1103 26-27 -0.7085290343069843 1103 28-29 -0.7598825756545153 1103 30-31 -0.7973785931125761 1103 32-33 -0.8564016740262943 1103 34-35 -0.9539785787909238 1103 36-37 -0.9828760282533366 1103 38-39 -0.9996093776279196 1103 40-41 -0.9733519405288362 1103 42-43 -0.8679753661379905 1103 44-45 -0.9640233406450278 1103 46-47 -0.9420029698232568 1103 48-49 -0.9939395911191653 1103 50-51 -0.9128005772877046 1103 52-53 -0.8117191933237251 1103 54-55 -0.782669655990027 1103 56-57 -0.7747962986286296 1103 58-59 -0.8825888404994728 1103 60-61 -0.9376313562768175 1103 62-63 -0.9013128579403897 1103 64-65 -0.8949696168780576 1103 66-67 -0.8591012713783925 1103 68-69 -0.8373946115498256 1103 70-71 -0.9015425311067844 1103 72-73 -1.0430730019470396 1103 74-75 -0.9613527672492026 1103 76-77 -0.9223883806064777 1103 78-79 -0.8836653058202018 1103 80-81 -0.9611043921895828 1103 82-83 -1.006328488561202 1103 84-85 -0.97180844535497 1103 86-87 -1.0971128961422885 1103 88-89 -1.2047695684742727 1103 90-91 -1.1227999857459032 1103 92-93 -1.2570342926204745 1103 94-95 -1.4474240862307681 1103 96-97 -1.4396109932611765 1103 98-99 -1.4814194040636757 1103 100 -1.6463217117901223 1104 1 -0.4263105016892297 1104 2 -0.45621980792371275 1104 3 -0.49729643712768024 1104 4 -0.35618289133208236 1104 5 -0.33394080380459457 1104 6 -0.32439939290529196 1104 7 -0.36165195545649453 1104 8 -0.32411263471325213 1104 9 -0.4912104386049094 1104 10-11 -0.44077763714247453 1104 12-13 -0.46599690114158676 1104 14-15 -0.5857014002303416 1104 16-17 -0.5468016126806177 1104 18-19 -0.6715846809664612 1104 20-21 -0.6904012816230605 1104 22-23 -0.5663243478993181 1104 24-25 -0.6799711287728414 1104 26-27 -0.7200597315721993 1104 28-29 -0.7254897229612709 1104 30-31 -0.6692151527618577 1104 32-33 -0.8306037253481193 1104 34-35 -0.7714416936009201 1104 36-37 -0.7636824621687879 1104 38-39 -0.744029629193669 1104 40-41 -0.7768007557908092 1104 42-43 -0.8029775407003328 1104 44-45 -0.8609240491171306 1104 46-47 -0.821853343383637 1104 48-49 -0.8139455378345772 1104 50-51 -0.8057141092412863 1104 52-53 -0.6934636313709959 1104 54-55 -0.6683627674699792 1104 56-57 -0.6796247158521993 1104 58-59 -0.6639948498312336 1104 60-61 -0.6379504606574855 1104 62-63 -0.6336097520554489 1104 64-65 -0.6365172125921248 1104 66-67 -0.6785394987131781 1104 68-69 -0.5676704193062534 1104 70-71 -0.6046544501143742 1104 72-73 -0.6716961806361468 1104 74-75 -0.6289912952222103 1104 76-77 -0.6111663478305047 1104 78-79 -0.6618687778867312 1104 80-81 -0.732780595106437 1104 82-83 -0.8148236600060557 1104 84-85 -0.8653914712638304 1104 86-87 -0.9170629271822897 1104 88-89 -0.8874238401866812 1104 90-91 -0.9064694784752199 1104 92-93 -0.8408426569772054 1104 94-95 -0.902592510604812 1104 96-97 -0.9518619281007759 1104 98-99 -1.08562563057583 1104 100 -1.2427110264903618 1105 1 -0.40407217749723756 1105 2 -0.4292523106896553 1105 3 -0.4049638354940086 1105 4 -0.2779534328683013 1105 5 -0.23614855550535196 1105 6 -0.32811031661236 1105 7 -0.3138695645052465 1105 8 -0.2586527085109509 1105 9 -0.44970754613533614 1105 10-11 -0.514271632987672 1105 12-13 -0.4849145496197238 1105 14-15 -0.5302438133091343 1105 16-17 -0.6099482988461986 1105 18-19 -0.636236746007306 1105 20-21 -0.5841236221988524 1105 22-23 -0.5973181671949988 1105 24-25 -0.579093456990158 1105 26-27 -0.6697616939200728 1105 28-29 -0.648457821934926 1105 30-31 -0.6583852008421829 1105 32-33 -0.6154517677082296 1105 34-35 -0.6740445484065987 1105 36-37 -0.6785994250463077 1105 38-39 -0.6673362986458997 1105 40-41 -0.8403118695828553 1105 42-43 -0.8916238619969121 1105 44-45 -0.8858767853403791 1105 46-47 -0.9533098327553589 1105 48-49 -0.9209025083037261 1105 50-51 -0.923131086831269 1105 52-53 -0.7824386290170011 1105 54-55 -0.732545688952527 1105 56-57 -0.7556110391496702 1105 58-59 -0.7619932564718468 1105 60-61 -0.792487856787524 1105 62-63 -0.754428397449864 1105 64-65 -0.6779778067401026 1105 66-67 -0.6976598848858231 1105 68-69 -0.7094428808184574 1105 70-71 -0.6656930982747298 1105 72-73 -0.7332202664038974 1105 74-75 -0.8446631544063159 1105 76-77 -0.84331538946088 1105 78-79 -0.7039731524894925 1105 80-81 -0.6869876597353297 1105 82-83 -0.8040861023332155 1105 84-85 -0.760785993226591 1105 86-87 -0.7257820089482543 1105 88-89 -0.8741690542235432 1105 90-91 -0.8435983934607414 1105 92-93 -0.8443730220227543 1105 94-95 -0.9740543479556258 1105 96-97 -1.0385548025622988 1105 98-99 -0.8954546570817996 1105 100 -0.9105857087012339 1106 1 -0.5274691431378393 1106 2 -0.6191766270673646 1106 3 -0.552046165362956 1106 4 -0.454877052179242 1106 5 -0.44574864084853516 1106 6 -0.44299108458411496 1106 7 -0.4721267368400248 1106 8 -0.482446541881302 1106 9 -0.5578617294127497 1106 10-11 -0.6101214004859514 1106 12-13 -0.5822450542551465 1106 14-15 -0.6696448181374492 1106 16-17 -0.7774800760303364 1106 18-19 -0.7446411864833564 1106 20-21 -0.7182033917722563 1106 22-23 -0.7289509876411273 1106 24-25 -0.8038138630717384 1106 26-27 -0.7672146784472389 1106 28-29 -0.7921154459748152 1106 30-31 -0.8590339515290211 1106 32-33 -0.8333258492460445 1106 34-35 -0.7777421076499635 1106 36-37 -0.7889655531546111 1106 38-39 -0.9415104498903872 1106 40-41 -0.9848967787449681 1106 42-43 -0.9168385717131713 1106 44-45 -0.8992666962853519 1106 46-47 -0.9238681510854363 1106 48-49 -0.912540757687033 1106 50-51 -0.9421685720715729 1106 52-53 -0.8387993632606623 1106 54-55 -0.890223890344906 1106 56-57 -0.7853896729572654 1106 58-59 -0.7759133108050662 1106 60-61 -0.8031457811360525 1106 62-63 -0.7682212955524363 1106 64-65 -0.84458246320456 1106 66-67 -0.9203465219551603 1106 68-69 -0.8083876539867987 1106 70-71 -0.7822644906254084 1106 72-73 -0.8841867523982643 1106 74-75 -0.8124255700860807 1106 76-77 -0.7724767648013753 1106 78-79 -0.7294541029846009 1106 80-81 -0.815573589654548 1106 82-83 -0.8392994274147583 1106 84-85 -0.7706550593909434 1106 86-87 -0.8038526647755582 1106 88-89 -0.8555256784037226 1106 90-91 -0.787776662075494 1106 92-93 -0.7232362807587407 1106 94-95 -0.8578754392669872 1106 96-97 -0.859594528061205 1106 98-99 -0.880712242980298 1106 100 -0.9942865121547086 1107 1 -0.4430653960299509 1107 2 -0.4272288623615843 1107 3 -0.34404120362082224 1107 4 -0.2632088734744116 1107 5 -0.26795870353875983 1107 6 -0.21025160348968086 1107 7 -0.32393293875838225 1107 8 -0.291628393937053 1107 9 -0.4917748090314831 1107 10-11 -0.41474804999113246 1107 12-13 -0.4263397714951296 1107 14-15 -0.5713080144994578 1107 16-17 -0.5226959068430261 1107 18-19 -0.5460897826720696 1107 20-21 -0.5177513577885975 1107 22-23 -0.5573506586926911 1107 24-25 -0.628417676058632 1107 26-27 -0.6761851068703706 1107 28-29 -0.6354839527133009 1107 30-31 -0.633530806573674 1107 32-33 -0.6444619581076054 1107 34-35 -0.6601646333714797 1107 36-37 -0.6765229100624381 1107 38-39 -0.7244509066077924 1107 40-41 -0.7737035713846439 1107 42-43 -0.808865757138939 1107 44-45 -0.7770157275304896 1107 46-47 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0.8410673017168335 2311 18-19 0.7725323432093987 2311 20-21 0.7407793236071925 2311 22-23 0.856386619676762 2311 24-25 0.8977383699746326 2311 26-27 0.9459919076166514 2311 28-29 0.9643770276468331 2311 30-31 0.9650205468597974 2311 32-33 1.0000553514912198 2311 34-35 0.9916916319338398 2311 36-37 1.0487254611877646 2311 38-39 1.0595194923173992 2311 40-41 1.0565563826596716 2311 42-43 1.0333882776602294 2311 44-45 1.0781409082887237 2311 46-47 1.1158654966865598 2311 48-49 1.1339378883130635 2311 50-51 1.1949672500672364 2311 52-53 1.0583840739706787 2311 54-55 1.0324965286758996 2311 56-57 0.9874880860382547 2311 58-59 1.0207512614481757 2311 60-61 1.0171573688272204 2311 62-63 1.0220156541812102 2311 64-65 0.9550704390952092 2311 66-67 1.0458870832899834 2311 68-69 1.000246977743501 2311 70-71 0.9656331293126286 2311 72-73 0.96686034407265 2311 74-75 0.9784176446290687 2311 76-77 1.0158885044366244 2311 78-79 0.9891626281566772 2311 80-81 1.0704995605165237 2311 82-83 1.0577397367851091 2311 84-85 1.1033223852179184 2311 86-87 1.0901110252218835 2311 88-89 1.1541008491384765 2311 90-91 1.1966976533623885 2311 92-93 1.155873885990804 2311 94-95 1.2026055846172525 2311 96-97 1.237858316591744 2311 98-99 1.1669487616979026 2311 100 1.280893733971837 2312 1 0.4742688453366668 2312 2 0.40719787698628807 2312 3 0.49879945821884775 2312 4 0.3976897482389887 2312 5 0.3529633071784133 2312 6 0.42407141626220834 2312 7 0.4793398896705412 2312 8 0.42036375223349864 2312 9 0.4967471379434869 2312 10-11 0.567785764572541 2312 12-13 0.5378680395263444 2312 14-15 0.657862712339913 2312 16-17 0.6841990463211474 2312 18-19 0.5895740727290075 2312 20-21 0.6748471902815965 2312 22-23 0.6963726953323643 2312 24-25 0.7752929795128125 2312 26-27 0.8345318820886902 2312 28-29 0.8231845380900964 2312 30-31 0.8230031124202526 2312 32-33 0.7476185912220146 2312 34-35 0.8393977412399423 2312 36-37 0.8276891853697066 2312 38-39 0.869078699790137 2312 40-41 0.9035862184684404 2312 42-43 0.9263666369083126 2312 44-45 0.8896999272026207 2312 46-47 0.8498923880766753 2312 48-49 0.8097399885683458 2312 50-51 0.8013923922115893 2312 52-53 0.665095680491909 2312 54-55 0.5837029425770268 2312 56-57 0.6078121896585884 2312 58-59 0.6587527554142909 2312 60-61 0.7294619203472905 2312 62-63 0.7001584222248312 2312 64-65 0.7139780307638048 2312 66-67 0.6552095043853683 2312 68-69 0.6840700805515709 2312 70-71 0.6563241249032501 2312 72-73 0.6557962920884322 2312 74-75 0.6178695911363761 2312 76-77 0.6268553761005862 2312 78-79 0.6810357425683691 2312 80-81 0.7052883021038951 2312 82-83 0.6460541658869943 2312 84-85 0.712646619378976 2312 86-87 0.7279221472919701 2312 88-89 0.7240416706747972 2312 90-91 0.7679964610904015 2312 92-93 0.8456982361880669 2312 94-95 0.9137388957103099 2312 96-97 0.8739016996272504 2312 98-99 0.9404823541787586 2312 100 1.1002398699662663 2313 1 0.4476290229935138 2313 2 0.43906007726216245 2313 3 0.4753690337953955 2313 4 0.3603478305120973 2313 5 0.3592674553178483 2313 6 0.38714128356644295 2313 7 0.40912667519345547 2313 8 0.41523606776836175 2313 9 0.5054749200183863 2313 10-11 0.5758285312505933 2313 12-13 0.5454657395299662 2313 14-15 0.6840812643221241 2313 16-17 0.6742305425339978 2313 18-19 0.6794773872806985 2313 20-21 0.670049058017419 2313 22-23 0.6198503410840743 2313 24-25 0.5842700124495153 2313 26-27 0.7050227173544101 2313 28-29 0.7356137786361927 2313 30-31 0.8562343599170603 2313 32-33 0.8032172054073428 2313 34-35 0.857495959537232 2313 36-37 0.8932132351498936 2313 38-39 0.8519926516320524 2313 40-41 0.872169004951715 2313 42-43 0.7516126333737461 2313 44-45 0.8799530592425455 2313 46-47 0.9024446438221148 2313 48-49 0.9688578408467308 2313 50-51 0.8628481637476995 2313 52-53 0.8115509371276843 2313 54-55 0.7534488456522297 2313 56-57 0.7501074007116557 2313 58-59 0.779860788734986 2313 60-61 0.7622879666031466 2313 62-63 0.7477916277297751 2313 64-65 0.7443604396731729 2313 66-67 0.7557513878150814 2313 68-69 0.7725392292181965 2313 70-71 0.7100750484780747 2313 72-73 0.7411091311751434 2313 74-75 0.7417422507741733 2313 76-77 0.7373271949705469 2313 78-79 0.7601198858410925 2313 80-81 0.7349125362449627 2313 82-83 0.7325588615617136 2313 84-85 0.8198261866187657 2313 86-87 0.9129363515579989 2313 88-89 0.9046383177145962 2313 90-91 0.9242080464267453 2313 92-93 0.871056450928755 2313 94-95 0.9349286245845434 2313 96-97 1.0199198442333 2313 98-99 1.0451811910842927 2313 100 1.1543284540700007 2314 1 0.35605842877565763 2314 2 0.23033317539409026 2314 3 0.38676999820176405 2314 4 0.2774793581422017 2314 5 0.28588520983755217 2314 6 0.30928602906340785 2314 7 0.35925216076001476 2314 8 0.3654362189328495 2314 9 0.48952959878246816 2314 10-11 0.4969235281280646 2314 12-13 0.5035450510389907 2314 14-15 0.5717420381340617 2314 16-17 0.6117792316693453 2314 18-19 0.6015791275471898 2314 20-21 0.6284284026027791 2314 22-23 0.6393729778857775 2314 24-25 0.7137071587937598 2314 26-27 0.7544990050670393 2314 28-29 0.7084887129274691 2314 30-31 0.7319506145394001 2314 32-33 0.7576029501598427 2314 34-35 0.7628148107062245 2314 36-37 0.7170254442075503 2314 38-39 0.8429620427632898 2314 40-41 0.8601133746387788 2314 42-43 0.7632011723011445 2314 44-45 0.8256165073825912 2314 46-47 0.6893445297872702 2314 48-49 0.603567992630353 2314 50-51 0.7536046594068964 2314 52-53 0.7582156520373289 2314 54-55 0.765726979691749 2314 56-57 0.6817450184710623 2314 58-59 0.6573080589559481 2314 60-61 0.5397107064644189 2314 62-63 0.681979857070921 2314 64-65 0.7048537654731177 2314 66-67 0.7038540794101493 2314 68-69 0.6756450733267343 2314 70-71 0.6835041432249511 2314 72-73 0.7451840970509096 2314 74-75 0.7505633039908659 2314 76-77 0.7290510868029187 2314 78-79 0.7641763046403725 2314 80-81 0.7492752613280516 2314 82-83 0.8349388461588916 2314 84-85 0.8990473100047218 2314 86-87 0.8509425250481755 2314 88-89 0.7146975850168396 2314 90-91 0.7607382507905633 2314 92-93 0.9151893326361851 2314 94-95 0.9017848826367825 2314 96-97 0.9344563966048192 2314 98-99 0.9181056181053826 2314 100 0.976117667224937 2315 1 0.34483746072876187 2315 2 0.32695273998049856 2315 3 0.3474899892853287 2315 4 0.2544764870401721 2315 5 0.2825326583989707 2315 6 0.2595211261249162 2315 7 0.23744418181708227 2315 8 0.2689131490677781 2315 9 0.12675432866034697 2315 10-11 0.30697833295980814 2315 12-13 0.32131467834148 2315 14-15 0.41990942304261836 2315 16-17 0.2453376742386908 2315 18-19 0.39871850897798566 2315 20-21 0.35098248546160704 2315 22-23 0.28026556795809654 2315 24-25 0.47718480660360996 2315 26-27 0.5589188729863181 2315 28-29 0.4786147848537823 2315 30-31 0.559501397447022 2315 32-33 0.5905270039491768 2315 34-35 0.5051422092484117 2315 36-37 0.6731072873869479 2315 38-39 0.6946110289121279 2315 40-41 0.7611935924417779 2315 42-43 0.7512152330232524 2315 44-45 0.7004237145506451 2315 46-47 0.768655746954785 2315 48-49 0.669396779317502 2315 50-51 0.6088745386454661 2315 52-53 0.38814509932559105 2315 54-55 0.5704391849169284 2315 56-57 0.5166266202161296 2315 58-59 0.5211338888370705 2315 60-61 0.6345556350943511 2315 62-63 0.6523844907489007 2315 64-65 0.6564329484086358 2315 66-67 0.5587341572848246 2315 68-69 0.5763665816965187 2315 70-71 0.5669559496167409 2315 72-73 0.622761229455314 2315 74-75 0.40501062242707775 2315 76-77 0.5409480885275713 2315 78-79 0.5902949566420261 2315 80-81 0.637964155945177 2315 82-83 0.5398874524777248 2315 84-85 0.6139707014993512 2315 86-87 0.5867665158964677 2315 88-89 0.5293681515626609 2315 90-91 0.7050979057110993 2315 92-93 0.8155905758305728 2315 94-95 0.9054305395348408 2315 96-97 0.8520930126278508 2315 98-99 0.9042780306157034 2315 100 0.8687658631632118 2316 1 0.11847828295364948 2316 2 0.14031032478827044 2316 3 0.208963498439644 2316 4 0.10171076463821294 2316 5 0.05775296509657579 2316 6 -0.0677413814978749 2316 7 -0.03734301547326169 2316 8 0.05173712849779122 2316 9 0.12659717592313768 2316 10-11 0.12462822097467097 2316 12-13 0.14003214402261221 2316 14-15 0.05855825431030581 2316 16-17 0.1969769585328507 2316 18-19 0.19667346780993 2316 20-21 0.2513056542619907 2316 22-23 0.21526113961057547 2316 24-25 0.20999144618518528 2316 26-27 0.09572764683911572 2316 28-29 0.14327626216466172 2316 30-31 -0.4591140184186884 2316 32-33 -0.21702653904933555 2316 34-35 -0.3753561749075871 2316 36-37 -0.04944010764618412 2316 38-39 0.0747200677142672 2316 40-41 0.19027140205532334 2316 42-43 0.2685622649473558 2316 44-45 0.08543620609346902 2316 46-47 -0.033349597122402486 2316 48-49 0.14007364787128296 2316 50-51 0.28686371743069117 2316 52-53 -0.05326975273532497 2316 54-55 -0.02314935915689631 2316 56-57 0.018156372809983168 2316 58-59 -0.3739000183579364 2316 60-61 0.059502493818904156 2316 62-63 0.10082546265568482 2316 64-65 -0.22896258911671907 2316 66-67 -0.5376160532799474 2316 68-69 -0.49378343838410643 2316 70-71 -0.29211816215531883 2316 72-73 -0.4687158095572457 2316 74-75 -0.28988850032468605 2316 76-77 0.03789013055388324 2316 78-79 0.16655525639043134 2316 80-81 -0.04730709608913486 2316 82-83 0.1376147940258825 2316 84-85 0.275931737787932 2316 86-87 0.40839484370514256 2316 88-89 0.408282630634492 2316 90-91 -0.2416317369863492 2316 92-93 -0.2670451297575873 2316 94-95 0.11167339334013349 2316 96-97 0.40833634164887656 2316 98-99 0.46345089884766466 2316 100 0.37120946666000165 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 225.0 1 591.0 2 326.0 3 356.0 4 285.0 5 289.0 6 333.0 7 679.0 8 1653.0 9 3090.0 10 5067.0 11 8322.0 12 12725.0 13 16763.0 14 19974.0 15 23276.0 16 27780.0 17 33369.0 18 40316.0 19 48130.0 20 58665.0 21 72483.0 22 81594.0 23 84275.0 24 84095.0 25 87132.0 26 95693.0 27 109728.0 28 128321.0 29 153031.0 30 185227.0 31 227987.0 32 288382.0 33 377126.0 34 527835.0 35 731497.0 36 1083844.0 37 1543369.0 38 1356956.0 39 310008.0 40 207.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.347316168971176 18.988763772263077 13.35677849077749 23.307141567988253 2 15.90648150740472 19.712118231110043 40.54565451145126 23.835745750033972 3 19.215481723306127 25.654079207159537 29.07297126533158 26.057467804202755 4 12.953995822272232 16.184026928527135 37.7110879653725 33.150889283828135 5 13.755546694411528 37.66723741857334 33.90141157002584 14.675804316989291 6 31.375323847560054 36.293118085242156 17.884267910643306 14.447290156554478 7 28.279584138154213 32.13863611654118 21.69591830451096 17.88586144079365 8 26.741767899352105 34.816016968996756 19.708842307212272 18.73337282443887 9 27.202712599153923 15.8886300436656 20.29972054168696 36.608936815493514 10-11 25.7333462782262 25.88559993510521 27.247400535637524 21.133653251031067 12-13 26.127701985607803 23.5003574288221 27.661912190545586 22.71002839502451 14-15 23.600258237780075 24.87995060065576 25.417241280642745 26.102549880921423 16-17 22.740511336294244 27.707004050460405 26.26781122480981 23.284673388435536 18-19 22.470288461581454 27.001084495823136 28.41182967200756 22.11679737058785 20-21 23.55207471758223 26.166139916092963 28.17717599857114 22.104609367753667 22-23 23.62577136898828 25.847730549600744 27.94946211220269 22.57703596920829 24-25 23.067362899726184 26.030804530489927 28.239569154680993 22.662263415102892 26-27 22.932294704616268 26.26920995688281 27.95501603877672 22.8434792997242 28-29 22.588113587596972 26.466371834110912 28.206581930574682 22.738932647717427 30-31 23.325447077015184 26.1274422939015 28.140807620275098 22.40630300880822 32-33 23.276360752362056 26.33753935479849 27.757550678964492 22.628549213874965 34-35 23.290440478978297 26.37198941003609 28.005511656408487 22.332058454577126 36-37 23.147181858423192 26.259839175039108 27.857849590528076 22.735129376009628 38-39 23.152553219228928 26.361194289750312 27.939778189680343 22.546474301340417 40-41 23.107566514163675 25.878033596228768 28.234624833381194 22.779775056226363 42-43 22.91947668106233 26.073294268423496 27.878937408728106 23.128291641786067 44-45 22.825292813299942 26.41677734025119 27.897595012111697 22.860334834337166 46-47 22.898010656673875 26.217320324595327 27.519923364867044 23.364745653863757 48-49 23.208461985038337 26.223213138583258 27.506044918470344 23.06227995790806 50-51 23.059272166851038 26.3696669832803 27.379974941781676 23.191085908086986 52-53 22.854539190863836 26.64614943076586 27.224762828692562 23.274548549677746 54-55 22.781000204233347 26.49370310322894 27.342437357952576 23.382859334585138 56-57 23.108943919214088 26.39382314959421 27.04740923576055 23.449823695431157 58-59 22.809697994633463 26.448215577317956 27.43438475393492 23.30770167411366 60-61 23.045376981855647 26.656641976090988 27.19819629672028 23.099784745333082 62-63 23.04986935328278 26.448134211551853 27.15883318746047 23.343163247704897 64-65 22.70977712019462 26.30692097758425 27.2939792810197 23.689322621201438 66-67 22.894953692012482 26.226489138606972 27.138696793822596 23.739860375557946 68-69 22.562683366259375 26.343863895670268 27.34931616200264 23.744136576067717 70-71 22.77135049091825 26.005825956760965 27.437923201531646 23.784900350789144 72-73 22.874785550562535 26.396193375142335 27.13415563255245 23.594865441742684 74-75 22.97440609662837 26.53529977800232 27.04554117868242 23.44475294668689 76-77 22.84417848769423 26.369938863549784 26.960624512853947 23.825258135902033 78-79 22.69822716569805 26.288017771468347 27.13259696570195 23.881158097131657 80-81 22.688282592781565 26.592926465907375 27.03344586863079 23.68534507268027 82-83 23.111535638134324 26.255350321356268 27.399049886546134 23.234064153963278 84-85 23.13767793267483 26.398389030895007 26.872326737448965 23.591606298981198 86-87 22.645312652925213 26.636247086515137 27.18652231676381 23.53191794379584 88-89 22.874260854909213 26.125646311706646 27.227002924986344 23.773089908397793 90-91 23.12926560959853 26.015080667251127 27.258549173437256 23.597104549713084 92-93 23.10860340794228 26.198215230597093 27.161966938334984 23.531214423125647 94-95 22.7846467376979 25.997461779564613 27.318918560390276 23.89897292234721 96-97 22.887578563556936 25.916902035989636 27.24211604087276 23.953403359580662 98-99 22.707770606729007 26.052738211760957 27.153014966373632 24.086476215136404 100 23.074257606144627 25.888209860481293 26.739630076916537 24.297902456457543 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1768.0 1 1297.0 2 1500.0 3 2000.0 4 1447.0 5 1213.5 6 1822.0 7 3242.5 8 5336.5 9 6635.0 10 6106.0 11 5272.5 12 5420.5 13 5976.5 14 6613.0 15 7358.5 16 7839.5 17 8389.5 18 9167.0 19 9752.5 20 10421.5 21 11334.0 22 12589.0 23 14270.5 24 16216.0 25 19028.5 26 23052.5 27 27915.0 28 33550.5 29 42350.5 30 55658.0 31 69455.0 32 87722.5 33 109617.0 34 130856.0 35 152654.0 36 178694.5 37 208744.0 38 224266.5 39 227795.0 40 227230.0 41 223865.5 42 226541.5 43 237617.0 44 252258.5 45 275628.5 46 297247.0 47 310397.0 48 324096.5 49 330623.5 50 328949.0 51 327318.5 52 321646.5 53 312919.0 54 304234.0 55 283011.5 56 257416.5 57 226956.0 58 196016.5 59 171657.0 60 143518.0 61 116269.0 62 93772.5 63 74488.0 64 56412.5 65 40441.5 66 29495.5 67 22300.0 68 16735.0 69 12179.5 70 9226.0 71 6990.0 72 5374.0 73 4246.5 74 3209.0 75 2308.5 76 1593.0 77 1025.0 78 676.5 79 434.0 80 292.0 81 214.0 82 152.5 83 117.5 84 88.5 85 55.5 86 48.5 87 50.5 88 37.5 89 33.5 90 30.5 91 23.5 92 19.0 93 13.5 94 14.5 95 12.0 96 6.0 97 4.5 98 1.5 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02493933089550203 2 0.11192689979471343 3 0.017022082992168053 4 0.01773718925440467 5 0.019997435833259695 6 0.04639251876260056 7 0.04715870404356836 8 0.03934361417769675 9 0.03377600113599738 10-11 0.03765800655956759 12-13 0.03667473544899224 14-15 0.03269057198795965 16-17 0.0383731128218042 18-19 0.037575003154129406 20-21 0.04814197515414371 22-23 0.04811005076743671 24-25 0.0673221466877044 26-27 0.05949428706715001 28-29 0.05204313520973811 30-31 0.04961049694266534 32-33 0.044643062371057404 34-35 0.04588172857528869 36-37 0.04136123541757864 38-39 0.04648829192272153 40-41 0.05595068014267392 42-43 0.05692118149856647 44-45 0.059839070443585524 46-47 0.061658760485884055 48-49 0.06132674686413134 50-51 0.05657001324478956 52-53 0.06673473797229576 54-55 0.0532818014139694 56-57 0.057348968280440164 58-59 0.0556122816435798 60-61 0.05102793971245577 62-63 0.058153462825456345 64-65 0.05572720943572497 66-67 0.05392028914810923 68-69 0.05906650028527632 70-71 0.05937935927500484 72-73 0.06204185312636796 74-75 0.05261777417046396 76-77 0.05321795264055541 78-79 0.06354868417893797 80-81 0.06404031973422565 82-83 0.05432892129795873 84-85 0.05949428706715001 86-87 0.05702972441337024 88-89 0.06583447026715859 90-91 0.07092960238559448 92-93 0.07610135303212716 94-95 0.07956195655116509 96-97 0.0752202399590142 98-99 0.0838142848605364 100 0.13011103046301598 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 7831004.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.46179686097503 #Duplication Level Percentage of deduplicated Percentage of total 1 72.62741897716016 39.5541973887102 2 15.281528156479588 16.64518964366923 3 5.314805932583849 8.683616431676597 4 2.4025919789057344 5.233979051798885 5 1.300154698207769 3.54043805308169 6 0.792258338917577 2.5888687629325555 7 0.4998662805459214 1.905653108012019 8 0.33224684192585013 1.447580801013291 9 0.2412062659787754 1.182287399339734 >10 1.1086696527123991 10.47085041012509 >50 0.06048890114825572 2.275027150034583 >100 0.03544325530637096 3.678082432497572 >500 0.0023343738972320337 0.8700617778789743 >1k 8.548333998137709E-4 0.7497984686284166 >5k 3.2878207675821336E-5 0.09506328674654059 >10k+ 9.8634623027464E-5 1.0793058338546773 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 26849 0.3428551434784097 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 21200 0.27071879927529086 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12227 0.15613579050655574 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.830926404839022E-5 2 1.276975468279674E-5 0.0 0.0 1.276975468279674E-5 3.830926404839022E-5 3 1.276975468279674E-5 0.0 0.0 3.830926404839022E-5 3.830926404839022E-5 4 1.276975468279674E-5 0.0 0.0 3.830926404839022E-5 6.38487734139837E-5 5 1.276975468279674E-5 0.0 0.0 3.830926404839022E-5 6.38487734139837E-5 6 1.276975468279674E-5 0.0 0.0 3.830926404839022E-5 8.938828277957718E-5 7 3.830926404839022E-5 0.0 0.0 3.830926404839022E-5 8.938828277957718E-5 8 3.830926404839022E-5 0.0 0.0 3.830926404839022E-5 8.938828277957718E-5 9 5.107901873118696E-5 0.0 0.0 3.830926404839022E-5 1.1492779214517066E-4 10-11 5.107901873118696E-5 0.0 0.0 5.107901873118696E-5 1.1492779214517066E-4 12-13 5.107901873118696E-5 0.0 0.0 5.746389607258533E-5 1.660068108763576E-4 14-15 5.107901873118696E-5 1.9154632024195112E-5 0.0 6.38487734139837E-5 2.2347070694894293E-4 16-17 5.107901873118696E-5 2.553950936559348E-5 0.0 8.30034054381788E-5 2.490102163145364E-4 18-19 5.107901873118696E-5 2.553950936559348E-5 0.0 1.0215803746237392E-4 2.553950936559348E-4 20-21 5.107901873118696E-5 2.553950936559348E-5 0.0 1.2131266948656903E-4 2.8731948036292665E-4 22-23 5.107901873118696E-5 2.553950936559348E-5 0.0 3.192438670699185E-4 3.8309264048390216E-4 24-25 6.38487734139837E-5 2.553950936559348E-5 0.0 9.130374598199668E-4 4.980204326290728E-4 26-27 7.661852809678043E-5 2.553950936559348E-5 0.0 0.0023496348616346002 5.810238380672516E-4 28-29 7.661852809678043E-5 2.553950936559348E-5 0.0 0.006040093964962858 5.874087154086501E-4 30-31 7.661852809678043E-5 2.553950936559348E-5 0.0 0.018516144290055273 5.874087154086501E-4 32-33 7.661852809678043E-5 2.553950936559348E-5 0.0 0.04916994040610885 6.001784700914468E-4 34-35 7.661852809678043E-5 2.553950936559348E-5 0.0 0.09586893328109652 6.257179794570402E-4 36-37 7.661852809678043E-5 2.553950936559348E-5 0.0 0.16901408810415625 6.321028567984387E-4 38-39 8.938828277957718E-5 2.553950936559348E-5 0.0 0.2784828101224313 6.512574888226338E-4 40-41 8.938828277957718E-5 2.553950936559348E-5 0.0 0.3992910742990299 6.704121208468289E-4 42-43 8.938828277957718E-5 2.553950936559348E-5 0.0 0.5174432295016067 6.89566752871024E-4 44-45 1.0215803746237392E-4 2.553950936559348E-5 0.0 0.6618116910679652 7.08721384895219E-4 46-47 1.276975468279674E-4 2.553950936559348E-5 0.0 0.8312216415672882 8.044945450161945E-4 48-49 1.276975468279674E-4 2.553950936559348E-5 0.0 1.0088106199409426 8.938828277957717E-4 50-51 1.276975468279674E-4 2.553950936559348E-5 0.0 1.195300117328506 9.258072145027636E-4 52-53 1.276975468279674E-4 2.553950936559348E-5 0.0 1.3767838708804132 0.001002425742599544 54-55 1.276975468279674E-4 2.553950936559348E-5 0.0 1.5845286249374921 0.0010662745160135278 56-57 1.532370561935609E-4 2.553950936559348E-5 0.0 1.8204434578248203 0.0010726593933549262 58-59 1.7877656555915437E-4 3.830926404839022E-5 0.0 2.0829513048390726 0.0010726593933549262 60-61 1.7877656555915437E-4 3.830926404839022E-5 0.0 2.3546597601022805 0.0010726593933549262 62-63 1.7877656555915437E-4 3.830926404839022E-5 0.0 2.6304417671093003 0.0011492779214517066 64-65 1.7877656555915437E-4 3.830926404839022E-5 0.0 2.9098184600595274 0.0011492779214517066 66-67 1.7877656555915437E-4 3.830926404839022E-5 0.0 3.222026192299225 0.0011620476761345032 68-69 1.7877656555915437E-4 4.469414138978859E-5 0.0 3.5679843861655542 0.0011875871855000968 70-71 1.7877656555915437E-4 5.107901873118696E-5 0.0 3.9350892937865947 0.0012386662042312836 72-73 1.915463202419511E-4 5.107901873118696E-5 0.0 4.296811749808837 0.001430212524473235 74-75 2.0431607492474784E-4 5.107901873118696E-5 0.0 4.649525143902366 0.001430212524473235 76-77 2.1708582960754458E-4 5.107901873118696E-5 0.0 5.031468506464816 0.00144936715649743 78-79 2.1708582960754458E-4 5.107901873118696E-5 0.0 5.445993898100422 0.0014685217885216251 80-81 2.2347070694894293E-4 5.107901873118696E-5 0.0 5.884373191483493 0.0015387554392770073 82-83 2.2985558429034132E-4 5.107901873118696E-5 0.0 6.33090597323153 0.0016983773728119665 84-85 2.2985558429034132E-4 5.107901873118696E-5 0.0 6.768634009125778 0.0016983773728119665 86-87 2.8093460302152826E-4 5.107901873118696E-5 1.276975468279674E-5 7.215779739098588 0.0017366866368603566 88 2.8093460302152826E-4 5.107901873118696E-5 1.276975468279674E-5 7.572349599106322 0.0017749959009087468 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 13835 0.0 51.70601 1 GTATCAA 25070 0.0 42.54805 1 CAACGCA 31270 0.0 33.13639 5 ATCAACG 31365 0.0 33.021255 3 TATCAAC 32310 0.0 32.574932 2 TCAACGC 33185 0.0 31.380367 4 AACGCAG 33525 0.0 31.300209 6 GTACTAG 3105 0.0 28.476538 1 ACGCAGA 37345 0.0 27.659172 7 GTACATG 37340 0.0 27.244143 1 CGCAGAG 38485 0.0 26.82747 8 TACATGG 38070 0.0 26.571615 2 GTGGTAT 5975 0.0 25.34597 1 ACATGGG 39110 0.0 25.172335 3 GCAGAGT 41675 0.0 24.266848 9 CATGGGG 27535 0.0 23.551798 4 TACCTGG 5290 0.0 22.669657 2 ATGGGGG 18810 0.0 22.384312 5 GAGTACT 21050 0.0 22.112436 12-13 TAGTACT 4595 0.0 21.885593 4 >>END_MODULE