FastQCFastQC Report
Mon 6 Jun 2016
ERR1211340_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211340_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7831004
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT234010.2988250293321265No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT194720.2486526631834181No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114150.1457667497041248No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA134550.050.682491
GTATCAA245600.041.4101331
CAACGCA301400.033.1686365
ATCAACG301950.033.1294673
TATCAAC314100.032.2193532
TCAACGC319750.031.4029964
AACGCAG323950.031.1779756
GTACTAG32400.027.9568561
ACGCAGA369550.027.0388557
CGCAGAG382050.026.0927948
GTACATG368650.025.8948121
TACATGG377200.025.001092
GTGGTAT62300.024.031381
GCAGAGT412950.023.787119
ACATGGG382500.023.7362353
TACCTGG51600.023.3678072
CATGGGG272200.022.184944
TGGTATC66900.021.9509222
ATGGGGG183150.021.797675
GAGTACT209900.021.5863912-13
Produced by FastQC (version 0.11.5)