##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211340_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7831004 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.13535480252596 33.0 31.0 34.0 30.0 34.0 2 32.27074753122333 34.0 31.0 34.0 30.0 34.0 3 32.33235597887576 34.0 31.0 34.0 30.0 34.0 4 35.966545413589365 37.0 35.0 37.0 35.0 37.0 5 35.872741094245384 37.0 35.0 37.0 35.0 37.0 6 35.88779727350414 37.0 35.0 37.0 35.0 37.0 7 35.80442775919921 37.0 35.0 37.0 35.0 37.0 8 35.786588156512245 37.0 35.0 37.0 33.0 37.0 9 37.406157115996876 39.0 37.0 39.0 34.0 39.0 10-11 37.356418921507384 39.0 37.0 39.0 34.0 39.0 12-13 37.280583945557936 39.0 37.0 39.0 34.0 39.0 14-15 38.514938633666894 40.0 38.0 41.0 34.0 41.0 16-17 38.495346318300946 40.0 38.0 41.0 34.0 41.0 18-19 38.502313688002204 40.0 38.0 41.0 34.0 41.0 20-21 38.46913984464827 40.0 38.0 41.0 34.0 41.0 22-23 38.36140998012515 40.0 38.0 41.0 34.0 41.0 24-25 38.268676277013775 40.0 38.0 41.0 34.0 41.0 26-27 38.16881258137526 40.0 38.0 41.0 33.0 41.0 28-29 38.067049640122775 40.0 37.5 41.0 33.0 41.0 30-31 38.07794396478408 40.0 37.0 41.0 33.0 41.0 32-33 38.01647700601353 40.0 37.0 41.0 33.0 41.0 34-35 37.891958234218755 40.0 37.0 41.0 33.0 41.0 36-37 37.75279242610526 40.0 37.0 41.0 33.0 41.0 38-39 37.64217864018458 40.0 37.0 41.0 33.0 41.0 40-41 37.52995126806218 40.0 36.5 41.0 32.0 41.0 42-43 37.421162024690574 40.0 36.0 41.0 32.0 41.0 44-45 37.257738854430414 40.0 36.0 41.0 31.5 41.0 46-47 37.01042082215767 39.0 36.0 41.0 31.0 41.0 48-49 35.4597015274159 39.0 35.0 41.0 24.5 41.0 50-51 36.17500444387463 39.0 35.0 40.0 29.0 41.0 52-53 36.26309148865203 39.0 35.0 40.0 29.5 41.0 54-55 36.08481639135927 38.0 35.0 40.0 29.0 41.0 56-57 35.859324040697715 38.0 35.0 40.0 28.5 41.0 58-59 35.60649247529436 38.0 34.0 40.0 28.0 41.0 60-61 35.34304528512563 37.0 34.0 40.0 28.0 41.0 62-63 35.01148460657152 37.0 34.0 40.0 27.5 41.0 64-65 34.671214955323734 36.0 33.5 39.0 27.0 41.0 66-67 34.32874788469013 36.0 33.0 39.0 26.0 40.0 68-69 33.971448871690015 35.0 33.0 38.5 26.0 40.0 70-71 33.61833565657737 35.0 33.0 38.0 26.0 40.0 72-73 33.247728643734575 35.0 32.0 37.0 26.0 39.5 74-75 32.88990287835378 35.0 32.0 36.5 25.0 39.0 76-77 31.96127201569556 34.0 30.5 35.5 24.5 38.0 78-79 32.406371520178 34.5 31.5 36.0 25.0 37.5 80-81 32.29310469002442 35.0 32.0 35.0 25.0 37.0 82-83 32.036869091115264 34.0 31.5 35.0 25.0 37.0 84-85 31.769502735536847 34.0 31.0 35.0 24.0 36.0 86-87 31.523539701933494 34.0 31.0 35.0 24.0 36.0 88-89 31.311026210687672 34.0 31.0 35.0 23.5 35.5 90-91 31.102475621261334 34.0 31.0 35.0 23.0 35.0 92-93 30.917298663108845 34.0 30.0 35.0 22.0 35.0 94-95 30.730604530402488 34.0 30.0 35.0 20.0 35.0 96-97 30.549052458663027 34.0 30.0 35.0 20.0 35.0 98-99 30.338211294490463 34.0 29.0 35.0 19.5 35.0 100 27.849309743680376 31.0 25.0 34.0 15.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.29355210136886356 1101 2 -0.2800257627000313 1101 3 -0.31230127040224076 1101 4 -0.19522160728717353 1101 5 -0.14906113891041883 1101 6 -0.1850411061915267 1101 7 -0.2590236516114075 1101 8 -0.1927025027690803 1101 9 -0.3031580313260065 1101 10-11 -0.26877116088896713 1101 12-13 -0.37778896235968773 1101 14-15 -0.43055230616777607 1101 16-17 -0.4918520746682802 1101 18-19 -0.4796149503701983 1101 20-21 -0.521611989984315 1101 22-23 -0.5796877783233327 1101 24-25 -0.436500735227078 1101 26-27 -0.5270411505426082 1101 28-29 -0.559993827697653 1101 30-31 -0.5607757096678156 1101 32-33 -0.5031927228214528 1101 34-35 -0.479256495043316 1101 36-37 -0.4990790496950126 1101 38-39 -0.4657486633026977 1101 40-41 -0.4925006706479991 1101 42-43 -0.5109265062825514 1101 44-45 -0.5389260686612118 1101 46-47 -0.5530585407067576 1101 48-49 0.623533205275173 1101 50-51 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1.070246554601642 2310 98-99 1.0980415440946913 2310 100 1.3854027664982702 2311 1 0.3370358285059325 2311 2 0.3543107265558021 2311 3 0.3647060575790064 2311 4 0.2449784469941747 2311 5 0.2860647898708848 2311 6 0.3386627300833567 2311 7 0.38166729481133643 2311 8 0.18081213549297104 2311 9 0.43878433604854195 2311 10-11 0.3593111736807728 2311 12-13 0.46411121897146046 2311 14-15 0.4138180695928497 2311 16-17 0.454645402035986 2311 18-19 0.6162555374931955 2311 20-21 0.6786935392391129 2311 22-23 0.6705737948129098 2311 24-25 0.6327620612060869 2311 26-27 0.7033438143121842 2311 28-29 0.7213241469002512 2311 30-31 0.6393690405790124 2311 32-33 0.6217881017042757 2311 34-35 0.7171234704211642 2311 36-37 0.8094479395391829 2311 38-39 0.7775173034755483 2311 40-41 0.7699766629580509 2311 42-43 0.7971599387221957 2311 44-45 0.8458612900794549 2311 46-47 0.9232056638945707 2311 48-49 -5.9680665602923675 2311 50-51 -1.0504767210944763 2311 52-53 0.3857324149204757 2311 54-55 0.8434378399355822 2311 56-57 0.9609278887296995 2311 58-59 1.0107769530252213 2311 60-61 0.9175505410282838 2311 62-63 0.7607993580384615 2311 64-65 0.8391081285537112 2311 66-67 0.9573904242155251 2311 68-69 0.9811721520279448 2311 70-71 0.9895067349857172 2311 72-73 0.49300306662064486 2311 74-75 0.8200773427655008 2311 76-77 0.8869719672940555 2311 78-79 0.8841390644219231 2311 80-81 0.9377223953352782 2311 82-83 0.9469927042365143 2311 84-85 0.9748752293017731 2311 86-87 0.957803193495014 2311 88-89 0.9552729138942411 2311 90-91 0.9889634149345419 2311 92-93 1.0288112918905057 2311 94-95 1.0637028596066251 2311 96-97 1.1261933882909076 2311 98-99 1.119343122885038 2311 100 1.3273595243316514 2312 1 0.0937115363267722 2312 2 0.22827365298884672 2312 3 0.18335960248501948 2312 4 0.012267841285186876 2312 5 0.17787916095466016 2312 6 0.09127354465750415 2312 7 0.25211649996334273 2312 8 0.2857958904245095 2312 9 0.2098412808153114 2312 10-11 0.1834111678789654 2312 12-13 0.28793200105547356 2312 14-15 0.3496083486599204 2312 16-17 0.44949448244907586 2312 18-19 0.32027800500305403 2312 20-21 0.34170627421242017 2312 22-23 0.5639981682174167 2312 24-25 0.6796551918628424 2312 26-27 0.5799701342240056 2312 28-29 0.6929025293555782 2312 30-31 0.7687185851369165 2312 32-33 0.7503163275107241 2312 34-35 0.3528912674738436 2312 36-37 0.689087574605324 2312 38-39 0.757889054461856 2312 40-41 0.5508640498227564 2312 42-43 0.5703708055126313 2312 44-45 0.7352987320613593 2312 46-47 0.7755387168070769 2312 48-49 -4.980127073172383 2312 50-51 -0.8526259291473934 2312 52-53 0.5240403169859178 2312 54-55 0.8021325776937616 2312 56-57 0.9058922801198079 2312 58-59 0.8609887190962411 2312 60-61 0.9313642884174769 2312 62-63 0.74470477402825 2312 64-65 0.9323033304567048 2312 66-67 0.8877995243547687 2312 68-69 0.7927619930583418 2312 70-71 0.8080605612795253 2312 72-73 0.9326533625129656 2312 74-75 0.6884397093238661 2312 76-77 0.8421384792457864 2312 78-79 0.4394121846354224 2312 80-81 0.6916404302621757 2312 82-83 0.7079363686598157 2312 84-85 0.7540819042843694 2312 86-87 0.8025268097632896 2312 88-89 0.7949145070713115 2312 90-91 0.6790198375384016 2312 92-93 0.7989471992936181 2312 94-95 0.9551727482118721 2312 96-97 1.0638873863182923 2312 98-99 1.0575284617107492 2312 100 0.6603580448700583 2313 1 0.013254780381863895 2313 2 0.18578790790887467 2313 3 0.2894971727416191 2313 4 0.06164339061057689 2313 5 0.10598789757905536 2313 6 0.2428401400085889 2313 7 0.15791313439374477 2313 8 0.24360268447303213 2313 9 0.42782629480564793 2313 10-11 0.21702227117614115 2313 12-13 0.04377474291798222 2313 14-15 0.11335524720911394 2313 16-17 0.07299857278393773 2313 18-19 0.4163674020788619 2313 20-21 0.39613184263878765 2313 22-23 0.47786836130061516 2313 24-25 0.26532983818975 2313 26-27 0.36396186173775646 2313 28-29 0.34003043296072377 2313 30-31 0.5885404052756869 2313 32-33 0.5153396514633997 2313 34-35 0.4289370130987109 2313 36-37 -0.28136613939492605 2313 38-39 0.22122594947608576 2313 40-41 0.37959799201385636 2313 42-43 0.44932731300706763 2313 44-45 0.5056126385704118 2313 46-47 0.458665936730803 2313 48-49 -5.479908154465068 2313 50-51 -1.430117772624378 2313 52-53 -0.006654013582654272 2313 54-55 0.17174258666788234 2313 56-57 0.19010907367064078 2313 58-59 0.3534106352218842 2313 60-61 0.6186247700081324 2313 62-63 0.7395104586558716 2313 64-65 0.7909724202860176 2313 66-67 0.8670524769910131 2313 68-69 0.8547093604078171 2313 70-71 0.8484223854863515 2313 72-73 0.6289100985110139 2313 74-75 0.8081110379123118 2313 76-77 0.768673019960584 2313 78-79 0.6668362021642693 2313 80-81 -0.2243844449322765 2313 82-83 0.2287075319982037 2313 84-85 0.6096740158242753 2313 86-87 0.7325997227497574 2313 88-89 0.6953156547630286 2313 90-91 0.7518197008414553 2313 92-93 0.8595693990619679 2313 94-95 0.7482887667172022 2313 96-97 0.8262609591050349 2313 98-99 1.0052855887773369 2313 100 1.066396539842426 2314 1 -0.02952913347373709 2314 2 0.13836705359565116 2314 3 0.1456308353476672 2314 4 0.05986782776126631 2314 5 0.18566946697772124 2314 6 0.2494499239971404 2314 7 0.09909602862641265 2314 8 0.211279370709768 2314 9 0.3902265672488028 2314 10-11 0.28900799706214997 2314 12-13 0.181934497950202 2314 14-15 0.15239925415305322 2314 16-17 0.36958519110067556 2314 18-19 0.48228853379050207 2314 20-21 0.497134637966532 2314 22-23 0.2752715588704504 2314 24-25 0.38203915578939274 2314 26-27 0.4262503670870572 2314 28-29 0.5474100864518974 2314 30-31 0.4410440115530818 2314 32-33 0.4418017720855758 2314 34-35 0.25249380976026004 2314 36-37 0.34177755127454645 2314 38-39 0.3992702470908327 2314 40-41 0.21851248394554545 2314 42-43 0.2207893553656035 2314 44-45 0.4864162859034806 2314 46-47 0.6494537464148138 2314 48-49 -6.4041038786363735 2314 50-51 -1.6241880538182514 2314 52-53 -0.9013798956858423 2314 54-55 -0.46281104394467576 2314 56-57 -0.13087776884605518 2314 58-59 0.3143499475354403 2314 60-61 0.521063494561119 2314 62-63 0.42511036773052524 2314 64-65 0.6383915942867304 2314 66-67 0.6892797462698823 2314 68-69 0.6369788778586596 2314 70-71 0.4064139719548123 2314 72-73 0.5688009021851528 2314 74-75 0.6762651274888327 2314 76-77 0.6481519262927797 2314 78-79 0.40289038243972186 2314 80-81 0.49432814539721903 2314 82-83 0.5140161020533611 2314 84-85 0.5340632880092144 2314 86-87 0.6069716539383059 2314 88-89 0.5657068485254442 2314 90-91 0.7623222414360278 2314 92-93 0.7978364187337768 2314 94-95 0.8729135555659902 2314 96-97 0.8455896868645638 2314 98-99 1.002088234335755 2314 100 -0.41696377088982217 2315 1 0.08307804818430498 2315 2 0.16326177723283308 2315 3 0.24881449176091053 2315 4 0.12533338754057155 2315 5 0.2338403049195179 2315 6 0.251578514150367 2315 7 0.30381294519356317 2315 8 0.2614531874048396 2315 9 0.3747339702923682 2315 10-11 0.19213658783721144 2315 12-13 0.3555022746829266 2315 14-15 0.18116468539831487 2315 16-17 0.34209458388149017 2315 18-19 0.4320700910302193 2315 20-21 0.3083123004408037 2315 22-23 0.37770324779545916 2315 24-25 0.4481956880046667 2315 26-27 0.561081475536696 2315 28-29 0.6290386400812551 2315 30-31 0.609497165688623 2315 32-33 0.5777091002817158 2315 34-35 0.6492782201052663 2315 36-37 0.5815397557992412 2315 38-39 0.656525265630215 2315 40-41 0.64306169575962 2315 42-43 0.6908021941382501 2315 44-45 0.7638304526966166 2315 46-47 0.6397849424156732 2315 48-49 -4.9147440494181325 2315 50-51 -0.9730174407991328 2315 52-53 0.2719067917041471 2315 54-55 0.24067293125595768 2315 56-57 0.5648261639868934 2315 58-59 0.6459792784630665 2315 60-61 0.6218799393978358 2315 62-63 0.34007316916176933 2315 64-65 0.5151731207637269 2315 66-67 -0.25796951953075364 2315 68-69 0.4896827949261606 2315 70-71 0.47109440561628446 2315 72-73 0.599758314543493 2315 74-75 0.716497683425132 2315 76-77 0.7335933931178893 2315 78-79 0.6554929690761995 2315 80-81 0.5397887728989517 2315 82-83 0.5613440560437368 2315 84-85 0.610707585677801 2315 86-87 0.6061634832130274 2315 88-89 0.6255621620431207 2315 90-91 0.6525633915477549 2315 92-93 0.7445747892451919 2315 94-95 0.4389663687583898 2315 96-97 0.4079398351895307 2315 98-99 0.43240686480717017 2315 100 0.44802965243526316 2316 1 0.2600914875062159 2316 2 0.2660703906174291 2316 3 0.21476274275703844 2316 4 0.02508894983019161 2316 5 0.19134872452661256 2316 6 0.1124795541213146 2316 7 0.2216118962266691 2316 8 0.29866567359871965 2316 9 0.4856477747788901 2316 10-11 0.39877044472566325 2316 12-13 0.2941313679204782 2316 14-15 0.4839250699119333 2316 16-17 0.45016018840784255 2316 18-19 0.20963475849745805 2316 20-21 0.48170426434385405 2316 22-23 0.6021023264516216 2316 24-25 0.6547345017468515 2316 26-27 0.6742079065715103 2316 28-29 0.7305131129288611 2316 30-31 0.6428227187457978 2316 32-33 0.6091691553050751 2316 34-35 0.5740615162059939 2316 36-37 0.49585482294501304 2316 38-39 0.5360676925591648 2316 40-41 0.18035231626095793 2316 42-43 0.18239319651410568 2316 44-45 0.4304738006378699 2316 46-47 0.42571208057149335 2316 48-49 -5.161793678075718 2316 50-51 -1.0809841341441953 2316 52-53 0.24183712949854197 2316 54-55 0.5341397537933901 2316 56-57 0.4749433079801264 2316 58-59 0.3166989827418405 2316 60-61 0.5002758807460381 2316 62-63 0.39722161837811143 2316 64-65 0.4160602490704193 2316 66-67 0.4649706468301176 2316 68-69 0.14849690843553276 2316 70-71 -0.24246146571828575 2316 72-73 -0.023928736225684588 2316 74-75 0.11656622500574088 2316 76-77 0.15764065741143796 2316 78-79 0.21168170354575722 2316 80-81 0.21727289682549156 2316 82-83 0.15603565319077006 2316 84-85 0.2982762294850403 2316 86-87 0.2829755636831841 2316 88-89 0.13441465751144221 2316 90-91 0.20817136818747883 2316 92-93 0.3634207178951989 2316 94-95 0.5519048132910989 2316 96-97 0.6046287530039542 2316 98-99 0.5346847837866449 2316 100 -0.2247779066334985 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 2.0 10 5.0 11 14.0 12 42.0 13 164.0 14 587.0 15 1761.0 16 4094.0 17 8615.0 18 14153.0 19 22041.0 20 31912.0 21 45879.0 22 57812.0 23 62313.0 24 65185.0 25 71527.0 26 84567.0 27 103118.0 28 126789.0 29 155379.0 30 190831.0 31 237975.0 32 299222.0 33 391798.0 34 550802.0 35 776155.0 36 1211853.0 37 1769377.0 38 1372643.0 39 174373.0 40 16.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.45895895441982 19.69124579894432 14.133391716899654 23.716403529736212 2 15.61064850328451 20.044051428919502 40.154443063438734 24.190857004357255 3 18.8392542459456 25.56627506065637 29.633929255522922 25.96054143787511 4 12.913950930917814 16.56985172497915 37.53152552504225 32.98467181906079 5 13.620375231181775 37.79033959953053 34.0154507257668 14.573834443520903 6 31.552156416254267 36.47055638601548 17.593176454303038 14.384110743427215 7 28.206113113764125 32.19187432104785 21.57904206626969 18.022970498918337 8 26.821741341347575 35.09343072069216 19.47161449480648 18.61321344315377 9 26.969270223849307 15.811261058593097 20.222284857021315 36.99718386053628 10-11 25.75498269166232 26.02700734065577 27.153626373850898 21.06438359383101 12-13 25.988276204695023 23.504096828545386 27.703255901084873 22.80437106567472 14-15 23.432747947689638 24.911941227365652 25.40374357448862 26.251567250456088 16-17 22.684746992075066 27.784159836955276 26.24038115280591 23.290712018163752 18-19 22.425667877495115 27.041571436196797 28.373789203476136 22.158971482831955 20-21 23.450278645891363 26.188289063850302 28.16133297978105 22.20009931047728 22-23 23.493650350964835 25.866589690388846 27.999348485452145 22.640411473194174 24-25 22.989446305849494 26.04939955321171 28.243384435350677 22.717769705588122 26-27 22.86897732530822 26.294204207266265 27.983324478567518 22.853493988858002 28-29 22.566860496298524 26.492159553933043 28.223881908309256 22.717098041459177 30-31 23.258966531076034 26.10991991049703 28.185888762472146 22.445224795954793 32-33 23.214507153720675 26.326932141853256 27.78583625756536 22.672724446860713 34-35 23.2030666824104 26.375680856486532 28.074548424986638 22.34670403611642 36-37 23.095161171559276 26.252169163353223 27.90092809186364 22.75174157322386 38-39 23.078199825286394 26.33772808066841 28.012534063182105 22.57153803086309 40-41 23.00534681233875 25.873455922607675 28.32808706611344 22.793110198940138 42-43 22.86972769540922 26.073207826527756 27.94493593710182 23.112128540961198 44-45 22.748053631491818 26.389605745638328 27.98308491227174 22.87925571059811 46-47 22.818444551538356 26.21088082514447 27.569561571758605 23.401113051558568 48-49 23.234307732528784 26.28855987187385 27.493781014962654 22.98335138063471 50-51 22.99726371298209 26.322853769572554 27.420823420082897 23.259059097362467 52-53 22.776723816036455 26.572301629992438 27.319556850296127 23.331417703674976 54-55 22.742197670403907 26.444267395839578 27.36259794796878 23.45093698578773 56-57 23.054445526696863 26.325750343821248 27.094129695160273 23.525674434321616 58-59 22.685107407629477 26.443948214770437 27.463579511056903 23.407364866543187 60-61 22.939693300876073 26.67805658974542 27.216974011973022 23.16527609740549 62-63 22.995317340322863 26.469376514214705 27.1231954417532 23.41211070370923 64-65 22.555942122908068 26.335707849851847 27.26059360337219 23.8477564238679 66-67 22.752758755912534 26.2660514950739 27.121281794829542 23.859907954184024 68-69 22.441701939698476 26.368482291611713 27.26939280142747 23.920422967262343 70-71 22.58723076609643 26.01326609786415 27.382525535025437 24.016977601013988 72-73 22.67516663088151 26.376880141614695 27.051495242575452 23.89645798492834 74-75 22.717017315000888 26.51028861399855 26.95584365396 23.816850417040563 76-77 22.531818956824814 26.37016441577068 26.9132850011718 24.184731626232704 78-79 22.393737682108444 26.265880960917663 27.054257947703974 24.28612340926992 80-81 22.329153524351756 26.614850205544645 26.89510962540707 24.16088664469653 82-83 22.714166436511498 26.228634283169082 27.28360810933781 23.77359117098161 84-85 22.75277020384291 26.318395803766887 26.753968550987295 24.174865441402908 86-87 22.143092207045996 26.599849013340304 27.082582269113253 24.174476510500444 88-89 22.326544817381595 26.09073594022514 27.105554790183973 24.477164452209294 90-91 22.516687356960606 25.99538365578112 27.100251099851068 24.387677887407207 92-93 22.466872414975565 26.14332393992047 27.014261256929316 24.375542388174644 94-95 22.029405216013856 25.945467082707495 27.210919277441626 24.814208423837027 96-97 22.06065239723961 25.902030909224067 27.20546408053068 24.831852613005637 98-99 21.81766096422143 26.003509157770203 27.130472953392548 25.048356924615817 100 21.851910044900904 25.886374523366996 26.941015667413946 25.32069976431815 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1542.0 1 1121.0 2 1219.0 3 1628.5 4 1227.5 5 1068.0 6 1595.0 7 2927.5 8 4727.0 9 5835.5 10 5400.0 11 4645.0 12 4790.0 13 5394.5 14 6060.5 15 6772.5 16 7584.5 17 8322.0 18 9031.5 19 9711.0 20 10436.0 21 11312.5 22 12566.0 23 14299.5 24 16209.5 25 18802.0 26 22763.5 27 27882.5 28 33780.0 29 42666.5 30 56020.5 31 69990.5 32 88019.5 33 109960.5 34 130817.5 35 152678.0 36 179714.5 37 208853.5 38 223305.5 39 226564.5 40 225218.0 41 221541.5 42 224405.0 43 235302.0 44 251681.0 45 276795.5 46 298353.0 47 311580.5 48 325615.5 49 332533.5 50 331646.5 51 330310.0 52 326199.0 53 318474.5 54 308632.0 55 287105.5 56 260927.5 57 229404.0 58 198332.0 59 174016.5 60 143464.0 61 114046.5 62 91522.5 63 72087.5 64 53769.0 65 37858.5 66 26991.5 67 20320.5 68 15218.5 69 10860.0 70 8129.5 71 6219.5 72 4805.0 73 3659.0 74 2701.0 75 1850.0 76 1210.5 77 728.5 78 416.5 79 230.0 80 130.5 81 79.5 82 55.0 83 38.5 84 21.5 85 12.0 86 8.5 87 4.5 88 6.5 89 4.5 90 1.0 91 2.0 92 1.0 93 1.5 94 1.5 95 0.0 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.029587521600040047 2 0.002004851485199088 3 5.107901873118696E-5 4 0.02086577915168987 5 5.746389607258533E-4 6 2.553950936559348E-5 7 3.8309264048390224E-5 8 0.0036010708205486803 9 0.009973178407264253 10-11 0.0012258964495484872 12-13 9.577316012097555E-5 14-15 1.5962193353495925E-4 16-17 3.064741123871218E-4 18-19 2.553950936559348E-5 20-21 9.577316012097555E-5 22-23 2.553950936559348E-4 24-25 2.553950936559348E-5 26-27 5.746389607258533E-5 28-29 9.194223371613653E-4 30-31 0.00258587532326634 32-33 0.009487927729317978 34-35 0.018330982847154717 36-37 0.021204177650783987 38-39 0.02068061770878932 40-41 0.004558802421758436 42-43 0.004846121902121363 44-45 0.009149529230223864 46-47 0.011601322129320838 48-49 1.1876510342735107 50-51 0.018081972630840184 52-53 0.019799504635676344 54-55 0.009736937945632514 56-57 0.008006636186113556 58-59 0.007974711799406564 60-61 0.005082362363753102 62-63 0.007981096676747963 64-65 0.019799504635676344 66-67 0.029293817242335717 68-69 0.029536442581308858 70-71 0.026822869711214555 72-73 0.023917750520878293 74-75 0.009839095983094888 76-77 0.014800145677361423 78-79 0.008638739042911995 80-81 0.0028540401716050713 82-83 0.014576674970412479 84-85 0.02409652708643745 86-87 0.023438884720273417 88-89 0.03272249637466664 90-91 0.021050940594590426 92-93 0.013395472662253782 94-95 0.006672196821761296 96-97 0.0038245415274976236 98-99 0.015387554392770071 100 0.03389092892814254 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 7831004.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.38899735547725 #Duplication Level Percentage of deduplicated Percentage of total 1 72.37549792405207 39.364307651926175 2 15.300742537718987 16.643840908416724 3 5.494646165676649 8.965448873228114 4 2.403293631555948 5.228509238845271 5 1.3410275500343958 3.6468571936221448 6 0.8049121933830674 2.6267020294381824 7 0.5000818100846466 1.9039263772351285 8 0.3390044931816279 1.4750491586520373 9 0.2412613510146296 1.1809766684082201 >10 1.1002872636548413 10.41584897889807 >50 0.059838603272330836 2.2295802787761416 >100 0.03596042157766079 3.6678925898494077 >500 0.0026176819259151047 0.9654319085874741 >1k 7.289681233402111E-4 0.7072634954446295 >5k 0.0 0.0 >10k+ 9.940474407633192E-5 0.9783646486722937 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 23401 0.2988250293321265 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19472 0.2486526631834181 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11415 0.1457667497041248 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 1.276975468279674E-5 6 0.0 0.0 0.0 0.0 5.107901873118696E-5 7 0.0 0.0 0.0 0.0 5.107901873118696E-5 8 0.0 0.0 0.0 0.0 6.38487734139837E-5 9 0.0 0.0 0.0 0.0 1.0215803746237392E-4 10-11 0.0 0.0 0.0 1.276975468279674E-5 1.1492779214517066E-4 12-13 0.0 0.0 0.0 1.9154632024195112E-5 1.5962193353495922E-4 14-15 0.0 0.0 0.0 2.553950936559348E-5 2.6177997099733317E-4 16-17 0.0 0.0 0.0 5.746389607258533E-5 3.0008923504572336E-4 18-19 0.0 0.0 0.0 6.38487734139837E-5 3.383984990941136E-4 20-21 0.0 0.0 0.0 8.30034054381788E-5 3.7670776314250383E-4 22-23 0.0 0.0 0.0 2.7454972568012994E-4 4.5332629123928425E-4 24-25 0.0 0.0 0.0 9.194223371613653E-4 5.171750646532679E-4 26-27 0.0 0.0 0.0 0.002407098757707185 5.68254083384455E-4 28-29 0.0 0.0 0.0 0.006103942738376842 5.746389607258533E-4 30-31 0.0 0.0 0.0 0.01860553257283485 5.810238380672516E-4 32-33 1.276975468279674E-5 0.0 0.0 0.049444490131788976 6.129482247742436E-4 34-35 1.276975468279674E-5 0.0 0.0 0.09647549662852936 6.512574888226338E-4 36-37 1.276975468279674E-5 0.0 0.0 0.16859907107696537 6.576423661640321E-4 38-39 1.276975468279674E-5 0.0 0.0 0.27345791165475075 6.89566752871024E-4 40-41 1.276975468279674E-5 0.0 0.0 0.3886602535256016 6.89566752871024E-4 42-43 1.276975468279674E-5 0.0 0.0 0.5017875102604978 7.08721384895219E-4 44-45 1.276975468279674E-5 0.0 0.0 0.6402180358993559 7.406457716022109E-4 46-47 2.553950936559348E-5 0.0 0.0 0.8026620852192132 7.789550356506012E-4 48-49 2.553950936559348E-5 0.0 0.0 0.9748175329753376 8.555735637473816E-4 50-51 2.553950936559348E-5 0.0 0.0 1.1609877864958311 8.874979504543734E-4 52-53 2.553950936559348E-5 0.0 0.0 1.341979904492451 9.577316012097555E-4 54-55 2.553950936559348E-5 0.0 0.0 1.549782122445602 0.0010279652519651376 56-57 3.8309264048390224E-5 0.0 0.0 1.7856011821728095 0.0010407350066479343 58-59 5.107901873118696E-5 0.0 0.0 2.0481409535737694 0.001072659393354926 60-61 5.107901873118696E-5 1.276975468279674E-5 0.0 2.319696171780783 0.0011109686574033164 62-63 6.38487734139837E-5 1.276975468279674E-5 0.0 2.595273862712878 0.001123738412086113 64-65 6.38487734139837E-5 1.276975468279674E-5 0.0 2.8737885972220165 0.001123738412086113 66-67 7.023365075538207E-5 1.276975468279674E-5 0.0 3.185721779736034 0.001123738412086113 68-69 7.661852809678043E-5 1.276975468279674E-5 0.0 3.5320056023467745 0.0011301232894275115 70-71 7.661852809678043E-5 1.276975468279674E-5 0.0 3.898887039260866 0.0012003569401828934 72-73 7.661852809678043E-5 1.276975468279674E-5 0.0 4.261152209857126 0.001430212524473235 74-75 7.661852809678043E-5 1.276975468279674E-5 0.0 4.614063535148238 0.001430212524473235 76-77 8.30034054381788E-5 1.276975468279674E-5 0.0 4.996524072775343 0.0014365974018146332 78-79 8.938828277957718E-5 1.276975468279674E-5 0.0 5.411700721899772 0.0014429822791560316 80-81 8.938828277957718E-5 1.276975468279674E-5 0.0 5.849543685586164 0.0014685217885216251 82-83 8.938828277957718E-5 1.276975468279674E-5 0.0 6.296382941446589 0.0016153739673737877 84-85 8.938828277957718E-5 1.276975468279674E-5 0.0 6.735260255262288 0.0016217588447151859 86-87 8.938828277957718E-5 1.276975468279674E-5 0.0 7.183121091497336 0.0016345285993979827 88 8.938828277957718E-5 1.276975468279674E-5 0.0 7.5401570475509905 0.0016472983540807794 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 13455 0.0 50.68249 1 GTATCAA 24560 0.0 41.410133 1 CAACGCA 30140 0.0 33.168636 5 ATCAACG 30195 0.0 33.129467 3 TATCAAC 31410 0.0 32.219353 2 TCAACGC 31975 0.0 31.402996 4 AACGCAG 32395 0.0 31.177975 6 GTACTAG 3240 0.0 27.956856 1 ACGCAGA 36955 0.0 27.038855 7 CGCAGAG 38205 0.0 26.092794 8 GTACATG 36865 0.0 25.894812 1 TACATGG 37720 0.0 25.00109 2 GTGGTAT 6230 0.0 24.03138 1 GCAGAGT 41295 0.0 23.78711 9 ACATGGG 38250 0.0 23.736235 3 TACCTGG 5160 0.0 23.367807 2 CATGGGG 27220 0.0 22.18494 4 TGGTATC 6690 0.0 21.950922 2 ATGGGGG 18315 0.0 21.79767 5 GAGTACT 20990 0.0 21.58639 12-13 >>END_MODULE