FastQCFastQC Report
Mon 6 Jun 2016
ERR1211339_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211339_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2432426
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131690.5413936539076626No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123740.5087102341448414No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79150.32539530493425084No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41600.17102267448218364No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52000.061.412391
GTATCAA89600.037.8457261
TACCTGG18050.036.4780352
ATCAACG110750.030.162593
GTACATG94550.030.0942441
CAACGCA112100.029.7580475
TATCAAC113750.029.3553712
GTACCTG23100.029.3183881
TCAACGC115450.029.0154574
TACATGG97000.028.800192
AACGCAG118900.028.570456
ACCTGGG23250.028.290973
ACATGGG97450.027.8669053
GTGGTAT19250.027.1195071
TAACGCA3150.026.8470024
CATGGGG65600.025.6397044
ATAACGC3200.024.9598333
ACGCAGA133850.024.8894487
TGGTATC20800.024.8719582
ATGGGGG47100.024.539515
Produced by FastQC (version 0.11.5)