##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211339_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2432426 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.389510718928346 31.0 31.0 34.0 28.0 34.0 2 31.386065187594607 31.0 31.0 34.0 28.0 34.0 3 31.427138585099815 31.0 31.0 34.0 28.0 34.0 4 35.05175820353836 37.0 35.0 37.0 32.0 37.0 5 35.10904586614351 37.0 35.0 37.0 32.0 37.0 6 35.17842474961211 37.0 35.0 37.0 32.0 37.0 7 35.11358002257828 37.0 35.0 37.0 32.0 37.0 8 35.11787038947948 37.0 35.0 37.0 32.0 37.0 9 36.68465186607938 39.0 37.0 39.0 32.0 39.0 10-11 36.61331917188848 39.0 37.0 39.0 32.0 39.0 12-13 36.525572412069266 39.0 37.0 39.0 32.0 39.0 14-15 37.64393572507447 40.0 37.0 41.0 32.0 41.0 16-17 37.64032759886632 40.0 37.0 41.0 32.0 41.0 18-19 37.668594029170876 40.0 37.0 41.0 32.0 41.0 20-21 37.63862826659475 40.0 37.0 41.0 32.0 41.0 22-23 37.507669503614906 40.0 37.0 41.0 31.5 41.0 24-25 37.397602845883085 40.0 37.0 41.0 31.0 41.0 26-27 37.24834260117265 40.0 36.5 41.0 31.0 41.0 28-29 37.10173012457522 40.0 36.0 41.0 30.5 41.0 30-31 36.88599838186239 39.0 36.0 41.0 30.0 41.0 32-33 36.70613391733191 39.0 35.0 41.0 30.0 41.0 34-35 36.52435880885996 39.0 35.0 41.0 30.0 41.0 36-37 36.40437818046674 39.0 35.0 41.0 29.5 41.0 38-39 36.23854888082926 39.0 35.0 41.0 29.0 41.0 40-41 36.060888594349834 39.0 35.0 40.0 28.0 41.0 42-43 35.90009089690704 39.0 35.0 40.0 27.0 41.0 44-45 35.73280646564376 38.5 35.0 40.0 26.5 41.0 46-47 35.52766497315848 38.0 34.5 40.0 25.5 41.0 48-49 35.25706167422976 38.0 34.0 40.0 24.0 41.0 50-51 34.56968721761731 37.5 33.5 39.5 23.5 40.5 52-53 34.800730011930476 38.0 33.5 40.0 24.0 40.5 54-55 35.161076842625434 38.0 34.0 40.0 24.5 41.0 56-57 35.066113624833804 38.0 34.0 40.0 24.5 41.0 58-59 34.85659152631982 38.0 34.0 40.0 24.0 41.0 60-61 34.60191882507423 37.5 33.5 40.0 23.5 41.0 62-63 34.33258175171619 37.0 33.0 40.0 23.0 41.0 64-65 34.06708097183635 36.5 33.0 40.0 23.0 41.0 66-67 33.84764675266585 36.0 33.0 39.5 22.5 41.0 68-69 33.51740546269444 36.0 32.5 39.0 22.0 41.0 70-71 33.175715931337685 35.0 32.0 39.0 22.0 40.5 72-73 32.815957402198464 35.0 32.0 38.0 21.0 40.0 74-75 32.44809996275323 35.0 31.0 37.0 20.0 39.5 76-77 32.09326717441764 35.0 31.0 37.0 20.0 39.0 78-79 31.72284871153326 35.0 31.0 36.0 19.5 39.0 80-81 31.394980977838586 35.0 30.5 36.0 18.5 38.5 82-83 31.07518090992285 34.5 30.0 35.5 18.0 37.0 84-85 30.76624448184652 34.0 30.0 35.0 18.0 37.0 86-87 30.500054266810174 34.0 29.5 35.0 17.0 36.0 88-89 30.235515283918197 34.0 29.0 35.0 16.0 36.0 90-91 29.968853317634327 34.0 29.0 35.0 15.0 36.0 92-93 29.72197139810214 34.0 29.0 35.0 12.5 35.0 94-95 29.48701111565162 34.0 29.0 35.0 11.0 35.0 96-97 29.27855235883846 34.0 27.5 35.0 10.0 35.0 98-99 29.031017593135413 34.0 27.0 35.0 10.0 35.0 100 27.38200751019764 32.0 24.0 34.0 8.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.33321652473092556 1101 2 -0.42360324999350496 1101 3 -0.4734878836158245 1101 4 -0.282161653470304 1101 5 -0.3158414993572336 1101 6 -0.31132798830763875 1101 7 -0.41084737657814685 1101 8 -0.5250989141993898 1101 9 -0.38116060789267436 1101 10-11 -0.5124320885040632 1101 12-13 -0.44857528547468206 1101 14-15 -0.6243586185661059 1101 16-17 -0.5725481692042749 1101 18-19 -0.7819321454400878 1101 20-21 -0.7657278199907793 1101 22-23 -0.6914848714157955 1101 24-25 -0.7664093993537975 1101 26-27 -0.8095615526453415 1101 28-29 -0.8914825098138124 1101 30-31 -0.8635587292241311 1101 32-33 -0.7909501778809584 1101 34-35 -0.882262887924945 1101 36-37 -0.7779021462880564 1101 38-39 -0.8395510691873582 1101 40-41 -0.9343290680483136 1101 42-43 -1.0893961601163582 1101 44-45 -1.0469058348557496 1101 46-47 -0.9915063973798155 1101 48-49 -0.7526433503450676 1101 50-51 -0.8466787214371578 1101 52-53 -0.746542367386013 1101 54-55 -0.7998761679078044 1101 56-57 -0.8328241766486073 1101 58-59 -0.8390279536187819 1101 60-61 -0.729315217853383 1101 62-63 -0.6911287181551558 1101 64-65 -0.8114622386580521 1101 66-67 -0.6449191532363585 1101 68-69 -0.750382658002998 1101 70-71 -0.6469739923830389 1101 72-73 -0.8854904553073908 1101 74-75 -0.7113706315805928 1101 76-77 -0.7251866640901277 1101 78-79 -0.862384127215531 1101 80-81 -1.1117059610887132 1101 82-83 -1.1842150638807354 1101 84-85 -0.8804640920465339 1101 86-87 -0.8479852964441825 1101 88-89 -0.9789416044989458 1101 90-91 -0.9343108885488967 1101 92-93 -0.9701641375538124 1101 94-95 -1.0874982029445235 1101 96-97 -1.1680965931156457 1101 98-99 -1.3344946780998832 1101 100 -0.9781923829534911 1102 1 -0.26879317885455833 1102 2 -0.5017010195760001 1102 3 -0.3787903081103785 1102 4 -0.41958164946393595 1102 5 -0.3166166143567182 1102 6 -0.3646771482440627 1102 7 -0.33430396726992484 1102 8 -0.3359150956084278 1102 9 -0.507958224173116 1102 10-11 -0.42509844968332544 1102 12-13 -0.47724742720370017 1102 14-15 -0.6149339039623953 1102 16-17 -0.5493445119831648 1102 18-19 -0.500146407143049 1102 20-21 -0.5550548673510391 1102 22-23 -0.5479768496180384 1102 24-25 -0.6102836159495624 1102 26-27 -0.5029786739673625 1102 28-29 -0.623430854755135 1102 30-31 -0.5669103099608037 1102 32-33 -0.6196309472961801 1102 34-35 -0.5813563842787133 1102 36-37 -0.6397843632270934 1102 38-39 -0.6310583282609414 1102 40-41 -0.7982869777894024 1102 42-43 -0.741612179022006 1102 44-45 -0.7381279443112518 1102 46-47 -0.6718985807879037 1102 48-49 -0.6797322887105821 1102 50-51 -0.7364852708147183 1102 52-53 -0.5694165433674101 1102 54-55 -0.4912605053990191 1102 56-57 -0.557973285323726 1102 58-59 -0.671425856081413 1102 60-61 -0.6022687524856281 1102 62-63 -0.6369200020351684 1102 64-65 -0.6573357379626756 1102 66-67 -0.4846457703137119 1102 68-69 -0.410798305964029 1102 70-71 -0.39331290487696435 1102 72-73 -0.44748451092338115 1102 74-75 -0.44939862704554656 1102 76-77 -0.3650725491725595 1102 78-79 -0.4247029830925584 1102 80-81 -0.5664581067093728 1102 82-83 -0.7453798519987629 1102 84-85 -0.7747424675541872 1102 86-87 -0.6270978287779307 1102 88-89 -0.842090324469261 1102 90-91 -0.9507014271895748 1102 92-93 -0.8079387406286216 1102 94-95 -0.6565538820386578 1102 96-97 -0.6933497847285217 1102 98-99 -0.7654731885069133 1102 100 -0.9024185816110126 1103 1 -0.5738786048019442 1103 2 -0.6254088209142594 1103 3 -0.5671643013049561 1103 4 -0.49963329318011773 1103 5 -0.4948668339439166 1103 6 -0.5477657298606999 1103 7 -0.6610410902687462 1103 8 -0.6253688903837755 1103 9 -0.7297146499170424 1103 10-11 -0.743134827693062 1103 12-13 -0.9229615446271353 1103 14-15 -0.9086249603607541 1103 16-17 -0.9638503852260598 1103 18-19 -0.9061137850399419 1103 20-21 -1.0304916306605278 1103 22-23 -1.0155730931072853 1103 24-25 -0.9266717145067247 1103 26-27 -0.9752967704338289 1103 28-29 -1.035501149412994 1103 30-31 -1.012222794790354 1103 32-33 -1.120442411269103 1103 34-35 -1.1210738886634388 1103 36-37 -1.0169206052448772 1103 38-39 -1.0780447676860447 1103 40-41 -0.9487628417525684 1103 42-43 -0.8060840812458778 1103 44-45 -1.0911687119074074 1103 46-47 -1.108198335277443 1103 48-49 -1.0143393824289504 1103 50-51 -1.0605765595407632 1103 52-53 -1.020565052925356 1103 54-55 -0.8319657692132765 1103 56-57 -0.6255772313558694 1103 58-59 -0.6321969345647176 1103 60-61 -0.7466547530168128 1103 62-63 -0.8116831488411052 1103 64-65 -0.8940238775353464 1103 66-67 -0.7108195073178294 1103 68-69 -0.7320044853452501 1103 70-71 -0.7228460293714107 1103 72-73 -0.7704803988011122 1103 74-75 -0.7299294466025827 1103 76-77 -0.7369628632356537 1103 78-79 -0.7610586835029096 1103 80-81 -0.8285615093562448 1103 82-83 -0.7862607208456005 1103 84-85 -0.8344756877155177 1103 86-87 -0.9113583404295902 1103 88-89 -0.9488973011176114 1103 90-91 -0.914594840393459 1103 92-93 -0.8525592264152699 1103 94-95 -1.24842418243567 1103 96-97 -1.0522867733967232 1103 98-99 -1.0381477379459128 1103 100 -0.8935384342572696 1104 1 -0.3432460385299194 1104 2 -0.3512093454597256 1104 3 -0.4184571428892596 1104 4 -0.4551343526434266 1104 5 -0.22205132873551037 1104 6 -0.251821325753248 1104 7 -0.2620264652298516 1104 8 -0.44968023923614453 1104 9 -0.37974502613764827 1104 10-11 -0.33563486473723714 1104 12-13 -0.3592040802265828 1104 14-15 -0.5652121549315652 1104 16-17 -0.5598288699712768 1104 18-19 -0.48014285730571515 1104 20-21 -0.5082236383064398 1104 22-23 -0.506557282654903 1104 24-25 -0.5301938743301022 1104 26-27 -0.4499659659826065 1104 28-29 -0.5043751547769872 1104 30-31 -0.4761869656677078 1104 32-33 -0.6455087203360321 1104 34-35 -0.5045232937340458 1104 36-37 -0.6552850309861284 1104 38-39 -0.5432047244184517 1104 40-41 -0.6933380932824917 1104 42-43 -0.6498117621325719 1104 44-45 -0.7260154231258653 1104 46-47 -0.76331858065803 1104 48-49 -0.7361265414373506 1104 50-51 -0.6785207562008608 1104 52-53 -0.5553436028683052 1104 54-55 -0.5976576802479912 1104 56-57 -0.3865171160744083 1104 58-59 -0.5593103045470826 1104 60-61 -0.4198807076451203 1104 62-63 -0.5196469048237446 1104 64-65 -0.49869588844114787 1104 66-67 -0.4603186701195554 1104 68-69 -0.4937178523640924 1104 70-71 -0.5414133089956437 1104 72-73 -0.49792272102613566 1104 74-75 -0.6280261170724515 1104 76-77 -0.7044380806849411 1104 78-79 -0.3975294111986116 1104 80-81 -0.4882041071898975 1104 82-83 -0.5852886817862277 1104 84-85 -0.6024124755413212 1104 86-87 -0.5320156522081554 1104 88-89 -0.6069210686580249 1104 90-91 -0.5913814807834612 1104 92-93 -0.5773914163185125 1104 94-95 -0.6017974246897353 1104 96-97 -0.7310671310952443 1104 98-99 -0.7681720389223976 1104 100 -1.1166791872911865 1105 1 -0.39998668045731733 1105 2 -0.43451438224523997 1105 3 -0.37628315954379943 1105 4 -0.31122215278895027 1105 5 -0.38434011521784583 1105 6 -0.3717966673330011 1105 7 -0.34705354906848385 1105 8 -0.38665776369244753 1105 9 -0.48527295887271293 1105 10-11 -0.5446584317192062 1105 12-13 -0.5894985664421242 1105 14-15 -0.6001786033105816 1105 16-17 -0.6363033222309511 1105 18-19 -0.7903094027014603 1105 20-21 -0.8075667541605966 1105 22-23 -0.7662700726945175 1105 24-25 -0.788091642945183 1105 26-27 -0.8286293583344531 1105 28-29 -0.8274479577521774 1105 30-31 -0.7557922288256052 1105 32-33 -0.9557763248311417 1105 34-35 -0.9735001714121196 1105 36-37 -1.031436094127855 1105 38-39 -0.8869794838977967 1105 40-41 -0.9720075491803044 1105 42-43 -1.0589929813095509 1105 44-45 -1.0771907404514423 1105 46-47 -0.9093621169180395 1105 48-49 -0.8544616938344731 1105 50-51 -0.8035773492965035 1105 52-53 -0.7466707109611193 1105 54-55 -0.6412515439394753 1105 56-57 -0.6618132192355333 1105 58-59 -0.6761123904068995 1105 60-61 -0.7007754849108636 1105 62-63 -0.8456130298136344 1105 64-65 -0.6710764365857074 1105 66-67 -0.6979399410387899 1105 68-69 -0.5884860227901569 1105 70-71 -0.6188377167893151 1105 72-73 -0.5984482283428108 1105 74-75 -0.6593726285123438 1105 76-77 -0.6775715191197378 1105 78-79 -0.6262170577343049 1105 80-81 -0.7891368826770027 1105 82-83 -0.6661642578230911 1105 84-85 -0.6304937674808464 1105 86-87 -0.696908731681031 1105 88-89 -0.7372185866465486 1105 90-91 -0.953906745558303 1105 92-93 -0.738790882726466 1105 94-95 -0.9503381312949557 1105 96-97 -1.0664974407403243 1105 98-99 -1.2234089214512984 1105 100 -1.0637019862240038 1106 1 -0.4929346837400175 1106 2 -0.5114348387262488 1106 3 -0.5558677122760258 1106 4 -0.4401881025264842 1106 5 -0.4396946312519674 1106 6 -0.45835347196594256 1106 7 -0.5861888984746528 1106 8 -0.4666089103145197 1106 9 -0.6245976054405915 1106 10-11 -0.6457932469125254 1106 12-13 -0.5491738192254516 1106 14-15 -0.6870145148015752 1106 16-17 -0.7929498204270189 1106 18-19 -0.8934271927635535 1106 20-21 -0.800389333557014 1106 22-23 -0.7728372131847578 1106 24-25 -0.9091622751744737 1106 26-27 -0.8537886544122415 1106 28-29 -0.8665037558971349 1106 30-31 -0.8244217406015153 1106 32-33 -0.8529442225100254 1106 34-35 -0.7570855138502921 1106 36-37 -0.8887639072617475 1106 38-39 -0.9805890594695583 1106 40-41 -0.8836500988466156 1106 42-43 -0.9351266351705902 1106 44-45 -0.9843129977717027 1106 46-47 -0.8720444460860577 1106 48-49 -0.839597608422352 1106 50-51 -0.8418738296854755 1106 52-53 -0.6165314285096244 1106 54-55 -0.6615220182275792 1106 56-57 -0.5605274308457595 1106 58-59 -0.4880218529423175 1106 60-61 -0.6977562145372218 1106 62-63 -0.5342881045260128 1106 64-65 -0.5817804362140748 1106 66-67 -0.75581551589827 1106 68-69 -0.729848226843977 1106 70-71 -0.776880910607936 1106 72-73 -0.7421778082502115 1106 74-75 -0.846376933183997 1106 76-77 -0.7956257843060932 1106 78-79 -0.7389245491017356 1106 80-81 -0.7848548570038183 1106 82-83 -0.6322278059254742 1106 84-85 -0.6759438611616524 1106 86-87 -0.7417677072311157 1106 88-89 -0.8015873638410795 1106 90-91 -0.7152943827890645 1106 92-93 -0.6795141604706956 1106 94-95 -0.9708441624805992 1106 96-97 -0.7931548554598216 1106 98-99 -0.8058886755585455 1106 100 -1.003160573114883 1107 1 -0.37535763466447136 1107 2 -0.35449571689612824 1107 3 -0.3918448061726636 1107 4 -0.13107604545535168 1107 5 -0.23396759249395416 1107 6 -0.22545660506288812 1107 7 -0.2205087561519008 1107 8 -0.3221989803908727 1107 9 -0.4772109789340391 1107 10-11 -0.38171297296727147 1107 12-13 -0.35636865012248364 1107 14-15 -0.5402008762481785 1107 16-17 -0.4181323499858607 1107 18-19 -0.37935056842309933 1107 20-21 -0.5015493357822791 1107 22-23 -0.5476542298912648 1107 24-25 -0.4872384678751942 1107 26-27 -0.4131964281099201 1107 28-29 -0.5224801711645526 1107 30-31 -0.4824229960655586 1107 32-33 -0.5334551553450595 1107 34-35 -0.4211177905605652 1107 36-37 -0.552360401431649 1107 38-39 -0.5007839996574717 1107 40-41 -0.6786818819725013 1107 42-43 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0.4545576139800289 2311 9 0.5901444834469132 2311 10-11 0.5960424294906232 2311 12-13 0.6631661540407592 2311 14-15 0.9546680524499394 2311 16-17 0.8458775788427388 2311 18-19 0.6878228249738427 2311 20-21 0.780308144769478 2311 22-23 0.8726778128285133 2311 24-25 0.8595529575700382 2311 26-27 0.8543107424534213 2311 28-29 0.8493301678942018 2311 30-31 0.9203296474083871 2311 32-33 0.9912301248407331 2311 34-35 0.917664436248522 2311 36-37 0.9102472608297489 2311 38-39 0.892990806243418 2311 40-41 0.8530796530159677 2311 42-43 0.900826550102046 2311 44-45 0.9597423277496091 2311 46-47 1.0940932994900763 2311 48-49 1.0288648231546773 2311 50-51 1.126454522988709 2311 52-53 1.027309187874934 2311 54-55 1.0452607912930318 2311 56-57 1.0977933601819245 2311 58-59 0.9732233686047778 2311 60-61 0.9923336612127542 2311 62-63 1.038977801498305 2311 64-65 0.9369616785305936 2311 66-67 1.0343956205339495 2311 68-69 1.0459264083412663 2311 70-71 0.981793382663497 2311 72-73 0.8906230565145634 2311 74-75 0.9801710995108195 2311 76-77 1.0291270668414114 2311 78-79 1.0472657180690845 2311 80-81 0.9991575739898515 2311 82-83 1.0434999079503982 2311 84-85 1.1426590032455373 2311 86-87 1.123143777673814 2311 88-89 1.1183971407749027 2311 90-91 1.2793264580294483 2311 92-93 1.3030830948219077 2311 94-95 1.2421591891766397 2311 96-97 1.2178096896257848 2311 98-99 1.264817606820074 2311 100 1.3492523583727518 2312 1 0.6226028816833278 2312 2 0.6178518273965103 2312 3 0.6438406794654874 2312 4 0.5726419191556218 2312 5 0.5979427979474252 2312 6 0.48748020130918945 2312 7 0.541483971202787 2312 8 0.5823677412463724 2312 9 0.8101641633445951 2312 10-11 0.8137043870925282 2312 12-13 0.8095984407818193 2312 14-15 0.9781182417432319 2312 16-17 0.9606983731840515 2312 18-19 0.8603005781329855 2312 20-21 0.8482934846717782 2312 22-23 0.9593085798191225 2312 24-25 0.933230043624178 2312 26-27 1.025620236240215 2312 28-29 0.9702024181876752 2312 30-31 1.0006687766292046 2312 32-33 0.9179705423367821 2312 34-35 1.0171492462496872 2312 36-37 1.0644267246838837 2312 38-39 1.1323961610970414 2312 40-41 1.1890679336444094 2312 42-43 1.1225712233196745 2312 44-45 1.0462207293996642 2312 46-47 1.0904222705211524 2312 48-49 1.1433913947693881 2312 50-51 1.108742052148287 2312 52-53 1.0398470540396119 2312 54-55 1.0346274918853808 2312 56-57 0.9354298885338821 2312 58-59 0.9558959392407544 2312 60-61 1.020092862428072 2312 62-63 1.0334817659994258 2312 64-65 1.047471772171484 2312 66-67 0.92173608726727 2312 68-69 1.0010300467955773 2312 70-71 1.1317501499363232 2312 72-73 1.166611479750948 2312 74-75 1.0140720125019627 2312 76-77 0.9893531454829443 2312 78-79 0.9926960338181061 2312 80-81 1.0258398975087566 2312 82-83 1.0465108122049145 2312 84-85 1.0867478660146581 2312 86-87 1.050006350141171 2312 88-89 1.1228543899124972 2312 90-91 1.2250479942323516 2312 92-93 1.2114910721607544 2312 94-95 1.0378271348114865 2312 96-97 1.060100303620576 2312 98-99 1.1725718392740418 2312 100 1.2842966869294692 2313 1 0.48870112055544723 2313 2 0.5201476786939807 2313 3 0.5230006003145604 2313 4 0.3982548742882699 2313 5 0.4270336791834737 2313 6 0.4380706703832047 2313 7 0.43049229153868396 2313 8 0.437613615178158 2313 9 0.5575525682478428 2313 10-11 0.6677623299591602 2313 12-13 0.6514219909499488 2313 14-15 0.7582163254368339 2313 16-17 0.7386179037344505 2313 18-19 0.7936547419580009 2313 20-21 0.6986265254392521 2313 22-23 0.6618767101058154 2313 24-25 0.8188675555096623 2313 26-27 0.8367117688306678 2313 28-29 0.7571685101996408 2313 30-31 0.9453373815447108 2313 32-33 0.7816538773503865 2313 34-35 0.8033631672450454 2313 36-37 0.8154353850287563 2313 38-39 0.8599983169911596 2313 40-41 0.9206791561150425 2313 42-43 0.727821421493509 2313 44-45 0.869694667316395 2313 46-47 0.8357921192369204 2313 48-49 0.8462178676309406 2313 50-51 0.827459620332931 2313 52-53 0.7577861351060591 2313 54-55 0.6906575115149636 2313 56-57 0.749479943983026 2313 58-59 0.7276306917162003 2313 60-61 0.8272443927649817 2313 62-63 0.7990814074085648 2313 64-65 0.6928215316942072 2313 66-67 0.5844956159199413 2313 68-69 0.6776703924186549 2313 70-71 0.6355663553175575 2313 72-73 0.7408737887677148 2313 74-75 0.763161697776205 2313 76-77 0.846449918560559 2313 78-79 0.8863327757414083 2313 80-81 0.8037277993173255 2313 82-83 0.827343152198079 2313 84-85 0.8610480059718384 2313 86-87 0.8715291900463633 2313 88-89 0.8691743363808513 2313 90-91 0.9257088866768406 2313 92-93 0.850359657703649 2313 94-95 0.9877235012620886 2313 96-97 1.1831637844822254 2313 98-99 1.0125804432802177 2313 100 1.1296262089994116 2314 1 0.39629227476746465 2314 2 0.15381771220031126 2314 3 0.34892095477230356 2314 4 0.22737222326485806 2314 5 0.30780019939921743 2314 6 0.4038245895639747 2314 7 0.3576282380724649 2314 8 0.3903087440339803 2314 9 0.5044202502092432 2314 10-11 0.5326344927207245 2314 12-13 0.5569959099313806 2314 14-15 0.5963500935607797 2314 16-17 0.5501461915811277 2314 18-19 0.5583260077329939 2314 20-21 0.6213000865547045 2314 22-23 0.6004060367143822 2314 24-25 0.6770820884035089 2314 26-27 0.7462889808307125 2314 28-29 0.8072470190111289 2314 30-31 0.6650290177976714 2314 32-33 0.7186877806616607 2314 34-35 0.6769115321492336 2314 36-37 0.8598366213328603 2314 38-39 0.861146169539964 2314 40-41 0.8110788375581564 2314 42-43 0.7552299792092114 2314 44-45 0.9216852710551109 2314 46-47 0.7148190840174706 2314 48-49 0.6490347647594774 2314 50-51 0.7484213392954544 2314 52-53 0.7204822947015046 2314 54-55 0.7399967563669207 2314 56-57 0.7325701621424656 2314 58-59 0.6965243556090215 2314 60-61 0.5370976922621793 2314 62-63 0.6608211792018466 2314 64-65 0.742946715507415 2314 66-67 0.7256733356078726 2314 68-69 0.9309044595300122 2314 70-71 0.9495747996032833 2314 72-73 1.005499291162117 2314 74-75 1.0069908128157437 2314 76-77 0.8902851596940735 2314 78-79 0.9177324107526204 2314 80-81 0.9231309014060081 2314 82-83 0.8884172096243752 2314 84-85 0.9828548706490956 2314 86-87 0.9027965792193697 2314 88-89 0.8226348015604472 2314 90-91 0.8820543896255053 2314 92-93 0.99199215478464 2314 94-95 1.0874733281148465 2314 96-97 1.0086674673100298 2314 98-99 1.031675806386204 2314 100 1.1144535516043774 2315 1 0.2758348373711037 2315 2 0.2646034758697233 2315 3 0.30480337201782604 2315 4 0.15360970824586673 2315 5 0.1914943934798572 2315 6 0.1562753639440615 2315 7 0.23267822041163555 2315 8 0.25882203241276613 2315 9 0.1811728630162861 2315 10-11 0.27408399027371644 2315 12-13 0.27686281479658703 2315 14-15 0.40889397234577274 2315 16-17 0.21706904557148476 2315 18-19 0.4250767354224152 2315 20-21 0.2841166150522909 2315 22-23 0.37353735313380554 2315 24-25 0.5148099604992495 2315 26-27 0.5591929389461257 2315 28-29 0.49831881284144686 2315 30-31 0.5313588735424446 2315 32-33 0.5283624423972313 2315 34-35 0.3560645726059022 2315 36-37 0.699847465617772 2315 38-39 0.7547273926023408 2315 40-41 0.5725421625461138 2315 42-43 0.6523279426505653 2315 44-45 0.724408683001414 2315 46-47 0.6434188570259849 2315 48-49 0.5780337284306398 2315 50-51 0.6461653077692944 2315 52-53 0.3623369103488514 2315 54-55 0.5486963069995809 2315 56-57 0.42559460745389543 2315 58-59 0.37517927316670807 2315 60-61 0.3957395597529825 2315 62-63 0.45224038369192243 2315 64-65 0.5417566864192764 2315 66-67 0.5405046807413285 2315 68-69 0.5920669552119762 2315 70-71 0.41871486530060054 2315 72-73 0.45349421592017336 2315 74-75 0.24559854413012516 2315 76-77 0.4330998613766326 2315 78-79 0.5384443754678756 2315 80-81 0.5386409982029114 2315 82-83 0.4228343317396721 2315 84-85 0.5782104242396393 2315 86-87 0.5008961207179503 2315 88-89 0.31960100110986644 2315 90-91 0.5108131465556234 2315 92-93 0.7690797282155586 2315 94-95 0.6478753148733531 2315 96-97 0.6638513950303455 2315 98-99 0.8828502030878305 2315 100 1.0612757437143827 2316 1 0.0013074499303797893 2316 2 0.06553254577439915 2316 3 0.031539380345016355 2316 4 -0.10324077960289912 2316 5 -0.024463572007967116 2316 6 -0.17750925126195938 2316 7 -0.17994287113909024 2316 8 -0.18795911377547014 2316 9 -0.11731938151367416 2316 10-11 -0.10293130010416007 2316 12-13 -0.09317429382642928 2316 14-15 -0.21153879391053465 2316 16-17 -0.02359575599025021 2316 18-19 0.02864229753679126 2316 20-21 0.1317354622858673 2316 22-23 -0.008887031758845865 2316 24-25 -0.03199009793038243 2316 26-27 -0.1740150705574024 2316 28-29 -0.15915776209842392 2316 30-31 -0.8050964306882875 2316 32-33 -0.5068285954253255 2316 34-35 -0.42609204203517237 2316 36-37 -0.3399490173824944 2316 38-39 -0.26430964056518746 2316 40-41 -0.003352758912456011 2316 42-43 0.06558556897562795 2316 44-45 -0.033691173633158655 2316 46-47 -0.19949369586540655 2316 48-49 -0.037142547202464016 2316 50-51 -0.08498121905320488 2316 52-53 -0.3324930342590804 2316 54-55 -0.2500767190170876 2316 56-57 -0.3222249223616629 2316 58-59 -0.623069288825782 2316 60-61 -0.15943460699590872 2316 62-63 -0.0710444066095306 2316 64-65 -0.4334527993031969 2316 66-67 -0.6993143905370189 2316 68-69 -0.7316661180901249 2316 70-71 -0.49050492493313413 2316 72-73 -0.7508433622681636 2316 74-75 -0.7041473740180813 2316 76-77 -0.3123948170765658 2316 78-79 -0.22703198084455067 2316 80-81 -0.2539652344205585 2316 82-83 -0.10515602737041974 2316 84-85 0.039641060329419275 2316 86-87 0.07086706068053061 2316 88-89 0.016571753401102285 2316 90-91 -0.3410665174159284 2316 92-93 -0.45893563761780953 2316 94-95 -0.11933675955284428 2316 96-97 0.10958100124942405 2316 98-99 0.1195717297652159 2316 100 0.21415340327856214 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 68.0 1 171.0 2 85.0 3 108.0 4 99.0 5 86.0 6 112.0 7 411.0 8 1090.0 9 1765.0 10 2474.0 11 3354.0 12 4697.0 13 5854.0 14 6707.0 15 7938.0 16 9804.0 17 12498.0 18 15831.0 19 19149.0 20 22458.0 21 27129.0 22 29995.0 23 30161.0 24 28896.0 25 29130.0 26 30432.0 27 34094.0 28 39414.0 29 46727.0 30 56352.0 31 69490.0 32 87124.0 33 113238.0 34 155815.0 35 217946.0 36 313604.0 37 456445.0 38 438097.0 39 113442.0 40 136.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.49459840339241 18.701642952223082 12.610921789421003 23.192836854963502 2 16.311946987334956 20.449065113042074 39.94404383315383 23.294944066469146 3 18.555695540475195 26.39226515702251 30.676626063730055 24.375413238772243 4 12.475657734590358 16.681812051395077 38.01044743715419 32.832082776860375 5 12.514227394889978 37.54757542164077 35.13471615935998 14.803481024109267 6 29.79996092585166 36.95501331605845 18.606821523686133 14.638204234403759 7 26.235012976757005 33.30485713228066 22.933651959675398 17.52647793128694 8 25.797906467150977 35.00860378500737 20.994921628237524 18.19856811960413 9 26.858006477150052 15.169855549272606 21.203680666221146 36.76845730735619 10-11 24.873691106743507 25.97823847365804 28.823843664444617 20.324226755153834 12-13 25.026449227456364 24.454755216383983 28.726626748296507 21.792168807863145 14-15 22.721515395516352 25.545404374420666 26.993620053937974 24.739460176125004 16-17 21.523301983265203 28.115946380053142 27.92929362512058 22.431458011561077 18-19 21.252639276046683 27.109590719947622 30.355334771654814 21.28243523235088 20-21 22.282162745100457 26.479548586989914 30.257734651311914 20.98055401659771 22-23 22.15574194437332 26.418136817892503 29.922644184843687 21.50347705289048 24-25 21.656971960771408 26.368969262160206 30.39269729480682 21.581361482261567 26-27 21.469412385599043 26.543366544181012 30.193440401672866 21.793780668547075 28-29 21.40302724371268 26.951411049959866 30.201361743469278 21.444199962858175 30-31 22.023243842763186 26.627117322978606 30.2631246106643 21.086514223593912 32-33 21.839613484447412 26.78434820948241 29.944919806330933 21.43111849973924 34-35 21.914863776036707 26.896526053467923 30.00277214870727 21.1858380217881 36-37 21.946888533040283 26.77782468654808 29.8011851856117 21.47410159479994 38-39 21.795506411465265 27.007782023548227 29.796486130822146 21.400225434164362 40-41 21.858772131704193 26.64489873820467 30.072648198891077 21.42368093120006 42-43 21.687765188364722 26.87489587853833 29.500031879163576 21.937307053933374 44-45 21.481796550076584 27.276328511755437 29.634779203379374 21.607095734788604 46-47 21.486833447726127 26.898994077010585 29.323579676517607 22.290592798745674 48-49 21.727619467599524 27.024001296611367 29.214624558609138 22.03375467717997 50-51 21.722971228968184 27.29656039621432 28.974519236800827 22.00594913801667 52-53 21.603416699439382 27.606582625908928 28.714698027548824 22.075302647102866 54-55 21.56600991735945 27.356647964588554 28.857085095929303 22.22025702212269 56-57 21.801974565483913 27.311716894622172 28.57550379637908 22.31080474351483 58-59 21.51239747608118 27.380899495709834 28.937576610149975 22.16912641805902 60-61 21.75358802130345 27.52054036412141 28.732756551723714 21.993115062851427 62-63 21.80103296249464 27.387964785818237 28.6908294550621 22.12017279662502 64-65 21.53717753517597 27.218251337821155 28.692091250107723 22.552479876895156 66-67 21.58471159110363 27.148911893100752 28.58675054470129 22.679625971094325 68-69 21.366251805829332 27.17135551555399 28.763652727912937 22.69873995070374 70-71 21.462741719290833 27.02648893159657 28.798375481001308 22.712393868111285 72-73 21.56567772599385 27.343167822909255 28.475037069077757 22.616117382019137 74-75 21.638371253642756 27.38017723794266 28.352230076155966 22.62922143225862 76-77 21.6096564662485 27.20713190851193 28.411249204854393 22.77196242038518 78-79 21.458762869634327 27.241459597945795 28.44131594839147 22.858461584028408 80-81 21.521461813098774 27.353275475451678 28.296445060037463 22.828817651412088 82-83 21.840971364299246 27.17492771509784 28.533281287244026 22.450819633358883 84-85 21.902515535704666 27.380232026731242 28.080783607492023 22.636468830072072 86-87 21.520847734443564 27.45842762397055 28.382950621151032 22.637774020434854 88-89 21.671729640684138 27.02151296020562 28.4339212471515 22.872836151958744 90-91 22.008633200074463 26.92396222126458 28.386772300119407 22.68063227854155 92-93 22.008645377824898 27.086190064192362 28.259655904582416 22.645508653400327 94-95 21.71656674308789 26.890561310289712 28.383480261621834 23.009391685000562 96-97 21.844284037218262 26.811477488785584 28.290317743306066 23.05392073069009 98-99 21.823460723790213 26.868539203794555 28.11443087390369 23.193569198511536 100 22.052321039143223 26.775325123302764 27.913472786951104 23.25888105060291 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1799.0 1 1306.0 2 1949.5 3 2695.0 4 1707.0 5 1275.0 6 1881.0 7 3379.0 8 5014.0 9 5634.0 10 4604.0 11 3370.0 12 3370.5 13 3656.5 14 3979.0 15 4232.5 16 4421.0 17 4782.0 18 5196.0 19 5493.0 20 5882.0 21 6591.0 22 7516.0 23 8765.5 24 10369.0 25 12489.5 26 14969.0 27 17636.5 28 20367.5 29 23835.5 30 28562.5 31 33140.0 32 38447.0 33 44527.0 34 50466.0 35 55727.0 36 61686.5 37 68354.0 38 71330.5 39 71851.0 40 71788.0 41 71489.5 42 72589.0 43 75134.5 44 78103.0 45 82636.5 46 86977.5 47 89590.5 48 92550.0 49 94464.5 50 94251.5 51 92690.5 52 89393.0 53 84778.5 54 80227.5 55 73813.0 56 66263.5 57 58023.0 58 49961.0 59 43534.0 60 36033.0 61 28650.0 62 23094.0 63 18252.5 64 13778.5 65 10273.0 66 7722.5 67 5893.0 68 4600.0 69 3513.5 70 2686.5 71 2031.5 72 1562.5 73 1269.5 74 987.0 75 689.0 76 501.0 77 371.5 78 247.0 79 170.0 80 124.5 81 81.0 82 55.5 83 41.5 84 31.5 85 26.0 86 24.5 87 22.5 88 15.5 89 12.0 90 11.0 91 7.5 92 7.5 93 8.5 94 5.0 95 4.0 96 3.0 97 1.0 98 1.0 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.026516736788703952 2 0.10964362327980379 3 0.017184489887873258 4 0.018171159163732013 5 0.0204733874740691 6 0.04731901402139264 7 0.04752457012052987 8 0.03856232419814622 9 0.03293008708178584 10-11 0.03646565198694637 12-13 0.03619842905806795 14-15 0.0305867475516213 16-17 0.03722620955375415 18-19 0.03650676320677381 20-21 0.04633234474553388 22-23 0.046394011575275056 24-25 0.0635579458532346 26-27 0.05726792921963504 28-29 0.0495184642821611 30-31 0.04939513062267876 32-33 0.04366011545674976 34-35 0.045181230590365336 36-37 0.04080288567874213 38-39 0.045941788157173126 40-41 0.05453403310110976 42-43 0.05636348238343119 44-45 0.059385157040748614 46-47 0.060618493635572056 48-49 0.060597938025658334 50-51 0.05416403212266273 52-53 0.0633935009739248 54-55 0.05075180087698454 56-57 0.055890703355415544 58-59 0.05286902869809811 60-61 0.04836735012699256 62-63 0.05591125896532926 64-65 0.0545751443209372 66-67 0.05249902771965108 68-69 0.057966819956701665 70-71 0.0587684887433369 72-73 0.06131738437263868 74-75 0.05060791160758847 76-77 0.05210847113129032 78-79 0.06207794193944646 80-81 0.06277683267651309 82-83 0.05128624673474137 84-85 0.058090153616184005 86-87 0.05486292285972934 88-89 0.06341405658383852 90-91 0.06962185077778317 92-93 0.0724790805557908 94-95 0.07476075325621417 96-97 0.07143074445019088 98-99 0.08214021721524108 100 0.1249781082754419 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2432426.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.60408206881957 #Duplication Level Percentage of deduplicated Percentage of total 1 76.77674317594351 50.36867760291281 2 15.583715230248124 20.44710665804623 3 4.10160962461362 8.072470032822366 4 1.4247700881904954 3.7388293517939424 5 0.6655892507786973 2.18326859161049 6 0.37334413383087794 1.4695739517451967 7 0.2354344383405049 1.0811822150301846 8 0.15793621831968274 0.8289008502626778 9 0.1121917980483135 0.6624215933948993 >10 0.5245760406881356 5.891823545551398 >50 0.030188459320552894 1.3698232723605595 >100 0.012670654321860356 1.4991401339098356 >500 6.838263087131418E-4 0.29940763503085116 >1k 3.419131543561546E-4 0.5863885522152519 >5k 6.838263087123092E-5 0.35508112545204795 >10k+ 1.3676526174246183E-4 1.1459048878612332 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13169 0.5413936539076626 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12374 0.5087102341448414 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7915 0.32539530493425084 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4160 0.17102267448218364 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 4.1111219827447986E-5 4.1111219827447986E-5 5 0.0 4.1111219827447986E-5 0.0 4.1111219827447986E-5 4.1111219827447986E-5 6 0.0 4.1111219827447986E-5 0.0 4.1111219827447986E-5 8.222243965489597E-5 7 0.0 4.1111219827447986E-5 0.0 4.1111219827447986E-5 8.222243965489597E-5 8 0.0 4.1111219827447986E-5 0.0 4.1111219827447986E-5 8.222243965489597E-5 9 0.0 4.1111219827447986E-5 0.0 8.222243965489597E-5 8.222243965489597E-5 10-11 0.0 4.1111219827447986E-5 0.0 8.222243965489597E-5 8.222243965489597E-5 12-13 0.0 4.1111219827447986E-5 0.0 8.222243965489597E-5 1.4388926939606797E-4 14-15 0.0 4.1111219827447986E-5 0.0 1.2333365948234397E-4 2.4666731896468794E-4 16-17 0.0 4.1111219827447986E-5 0.0 1.2333365948234397E-4 2.8777853879213594E-4 18-19 0.0 4.1111219827447986E-5 0.0 1.6444487930979194E-4 2.8777853879213594E-4 20-21 0.0 4.1111219827447986E-5 0.0 2.0555609913723994E-4 2.8777853879213594E-4 22-23 0.0 4.1111219827447986E-5 0.0 3.288897586195839E-4 2.8777853879213594E-4 24-25 0.0 4.1111219827447986E-5 0.0 0.0010894473254273716 2.8777853879213594E-4 26-27 0.0 4.1111219827447986E-5 0.0 0.0025077844094743276 2.8777853879213594E-4 28-29 0.0 4.1111219827447986E-5 0.0 0.008571689334022905 2.8777853879213594E-4 30-31 0.0 4.1111219827447986E-5 0.0 0.027832295823182286 2.8777853879213594E-4 32-33 0.0 4.1111219827447986E-5 0.0 0.06937518345881848 3.288897586195839E-4 34-35 0.0 4.1111219827447986E-5 0.0 0.13521480201247643 3.700009784470319E-4 36-37 0.0 4.1111219827447986E-5 0.0 0.24048008038065702 3.700009784470319E-4 38-39 0.0 4.1111219827447986E-5 0.0 0.39232437081333615 3.700009784470319E-4 40-41 0.0 4.1111219827447986E-5 0.0 0.5547959115714105 3.700009784470319E-4 42-43 0.0 4.1111219827447986E-5 0.0 0.710648545937266 3.700009784470319E-4 44-45 0.0 4.1111219827447986E-5 0.0 0.9030696103396363 3.700009784470319E-4 46-47 0.0 4.1111219827447986E-5 0.0 1.1333129969832587 3.700009784470319E-4 48-49 0.0 6.166682974117197E-5 0.0 1.365283054859634 3.700009784470319E-4 50-51 0.0 8.222243965489597E-5 0.0 1.6001514537338442 3.700009784470319E-4 52-53 0.0 8.222243965489597E-5 0.0 1.8376509706770112 3.9055658836075586E-4 54-55 0.0 8.222243965489597E-5 0.0 2.109622245445493 4.111121982744799E-4 56-57 0.0 1.2333365948234397E-4 0.0 2.4185525068388514 4.111121982744799E-4 58-59 0.0 1.2333365948234397E-4 0.0 2.753958393801086 4.111121982744799E-4 60-61 0.0 1.2333365948234397E-4 0.0 3.078860364097407 4.111121982744799E-4 62-63 0.0 1.2333365948234397E-4 0.0 3.42170738184841 4.111121982744799E-4 64-65 0.0 1.2333365948234397E-4 0.0 3.769056078170518 4.111121982744799E-4 66-67 0.0 1.2333365948234397E-4 0.0 4.161627116302819 4.111121982744799E-4 68-69 0.0 1.2333365948234397E-4 0.0 4.58649101760958 4.111121982744799E-4 70-71 0.0 1.2333365948234397E-4 0.0 5.029608300519728 4.111121982744799E-4 72-73 0.0 1.2333365948234397E-4 0.0 5.462509445302755 4.522234181019279E-4 74-75 0.0 1.2333365948234397E-4 0.0 5.899377822799131 4.933346379293759E-4 76-77 0.0 1.2333365948234397E-4 0.0 6.369916289334188 4.933346379293759E-4 78-79 0.0 1.2333365948234397E-4 0.0 6.883107646440221 4.933346379293759E-4 80-81 0.0 1.2333365948234397E-4 0.0 7.407008476311304 4.933346379293759E-4 82-83 0.0 1.2333365948234397E-4 0.0 7.924208177350513 4.933346379293759E-4 84-85 0.0 1.2333365948234397E-4 0.0 8.43879731593068 4.933346379293759E-4 86-87 0.0 1.2333365948234397E-4 0.0 8.973592619056037 4.933346379293759E-4 88 0.0 1.2333365948234397E-4 0.0 9.396997072058923 4.933346379293759E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5200 0.0 61.41239 1 GTATCAA 8960 0.0 37.845726 1 TACCTGG 1805 0.0 36.478035 2 ATCAACG 11075 0.0 30.16259 3 GTACATG 9455 0.0 30.094244 1 CAACGCA 11210 0.0 29.758047 5 TATCAAC 11375 0.0 29.355371 2 GTACCTG 2310 0.0 29.318388 1 TCAACGC 11545 0.0 29.015457 4 TACATGG 9700 0.0 28.80019 2 AACGCAG 11890 0.0 28.57045 6 ACCTGGG 2325 0.0 28.29097 3 ACATGGG 9745 0.0 27.866905 3 GTGGTAT 1925 0.0 27.119507 1 TAACGCA 315 0.0 26.847002 4 CATGGGG 6560 0.0 25.639704 4 ATAACGC 320 0.0 24.959833 3 ACGCAGA 13385 0.0 24.889448 7 TGGTATC 2080 0.0 24.871958 2 ATGGGGG 4710 0.0 24.53951 5 >>END_MODULE