FastQCFastQC Report
Mon 6 Jun 2016
ERR1211339_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211339_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2432426
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113810.46788679285618556No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112700.46332344745533877No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75510.31043082091705976No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32350.13299479614179424No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52350.059.34711
TACCTGG18250.039.5888752
GTATCAA89150.036.0074961
GTGGTAT20050.031.5993081
ACCTGGG23700.029.894313
GTACCTG25250.029.3666151
ATCAACG113200.027.6878993
TGGTATC22400.027.6465682
GTACATG98100.027.4121931
TAACGCA2750.027.299034
CAACGCA115050.027.2800945
TATCAAC117200.026.980352
TACATGG99600.026.5665442
TCAACGC119150.026.3446274
AACGCAG120850.026.1649326
ACATGGG99750.025.682643
CATGGGG65300.024.2145654
ATGGGGG43300.023.8364265
ATAACGC3200.023.4601063
ACGCAGA136800.022.9770937
Produced by FastQC (version 0.11.5)