##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211339_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2432426 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.09436751621632 33.0 31.0 34.0 30.0 34.0 2 32.21061031250283 33.0 31.0 34.0 30.0 34.0 3 32.26996299167991 34.0 31.0 34.0 30.0 34.0 4 35.92703868483564 37.0 35.0 37.0 35.0 37.0 5 35.83855007305464 37.0 35.0 37.0 35.0 37.0 6 35.862581225492576 37.0 35.0 37.0 35.0 37.0 7 35.77808862427881 37.0 35.0 37.0 33.0 37.0 8 35.76612320374803 37.0 35.0 37.0 33.0 37.0 9 37.38130903057277 39.0 37.0 39.0 34.0 39.0 10-11 37.32809240651103 39.0 37.0 39.0 34.0 39.0 12-13 37.25503920777035 39.0 37.0 39.0 33.5 39.0 14-15 38.4654519808619 40.0 38.0 41.0 34.0 41.0 16-17 38.45081720882773 40.0 38.0 41.0 34.0 41.0 18-19 38.4836817235139 40.0 38.0 41.0 34.0 41.0 20-21 38.460820596392246 40.0 38.0 41.0 34.0 41.0 22-23 38.34556755272308 40.0 38.0 41.0 34.0 41.0 24-25 38.24991757200425 40.0 38.0 41.0 34.0 41.0 26-27 38.13759637497708 40.0 37.5 41.0 33.0 41.0 28-29 38.01585577526305 40.0 37.0 41.0 33.0 41.0 30-31 37.97994286362668 40.0 37.0 41.0 33.0 41.0 32-33 37.86827410165818 40.0 37.0 41.0 33.0 41.0 34-35 37.70721596463777 40.0 37.0 41.0 33.0 41.0 36-37 37.538352656977025 40.0 36.0 41.0 32.5 41.0 38-39 37.40925623225537 40.0 36.0 41.0 32.0 41.0 40-41 37.28686586971197 40.0 36.0 41.0 31.5 41.0 42-43 37.157522983227445 40.0 36.0 41.0 31.0 41.0 44-45 36.97175412530535 39.0 35.5 41.0 31.0 41.0 46-47 36.69352675065963 39.0 35.0 41.0 30.0 41.0 48-49 35.113820112102076 39.0 35.0 40.5 23.0 41.0 50-51 35.83452610685792 39.0 34.5 40.0 27.5 41.0 52-53 35.9346958550846 39.0 35.0 40.0 28.0 41.0 54-55 35.76698468935951 38.0 35.0 40.0 28.0 41.0 56-57 35.55995454743536 38.0 34.5 40.0 27.5 41.0 58-59 35.33383893281851 38.0 34.0 40.0 27.0 41.0 60-61 35.08585461592665 37.5 34.0 40.0 26.5 41.0 62-63 34.77155173477014 37.0 34.0 40.0 26.0 41.0 64-65 34.44416582457185 36.5 33.5 39.5 26.0 41.0 66-67 34.11722946556236 36.0 33.0 39.0 26.0 40.5 68-69 33.76062170031072 35.5 33.0 39.0 25.0 40.0 70-71 33.40339089452259 35.0 32.0 38.0 25.0 40.0 72-73 33.02389342985151 35.0 32.0 37.5 24.0 40.0 74-75 32.660700469407914 35.0 32.0 37.0 24.0 39.0 76-77 31.703827372343497 34.0 30.5 36.0 22.5 38.5 78-79 32.13612109885358 35.0 31.0 36.0 24.0 38.5 80-81 32.00611179949565 35.0 31.0 36.0 23.5 37.5 82-83 31.73092768289765 34.0 31.0 35.0 23.0 37.0 84-85 31.435724046692478 34.0 31.0 35.0 21.5 36.5 86-87 31.160157595750086 34.0 31.0 35.0 20.0 36.0 88-89 30.92916495712511 34.0 30.5 35.0 20.0 36.0 90-91 30.702571424577766 34.0 30.0 35.0 20.0 35.0 92-93 30.500437012266765 34.0 30.0 35.0 19.0 35.0 94-95 30.29367409327149 34.0 29.5 35.0 19.0 35.0 96-97 30.09979275834085 34.0 29.0 35.0 18.0 35.0 98-99 29.872804763639266 34.0 29.0 35.0 18.0 35.0 100 27.51546069644051 31.0 25.0 34.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.22377586860016052 1101 2 -0.22425239400923047 1101 3 -0.28788000131342173 1101 4 -0.11718184449893698 1101 5 -0.2195539477882491 1101 6 -0.23743541791075984 1101 7 -0.34555173858773003 1101 8 -0.22021041898248228 1101 9 -0.4213400169389985 1101 10-11 -0.36327747205383787 1101 12-13 -0.43923212375865006 1101 14-15 -0.4802339369364077 1101 16-17 -0.45765028429478605 1101 18-19 -0.5363996260519173 1101 20-21 -0.444549118761671 1101 22-23 -0.5513769177201269 1101 24-25 -0.5741154799070713 1101 26-27 -0.7059648078272431 1101 28-29 -0.6338300575546683 1101 30-31 -0.6056979324490825 1101 32-33 -0.507102549910698 1101 34-35 -0.5609726999065643 1101 36-37 -0.5525937480039715 1101 38-39 -0.43280437596394705 1101 40-41 -0.524123902137319 1101 42-43 -0.47370851764470956 1101 44-45 -0.41024341878507187 1101 46-47 -0.44945395352417705 1101 48-49 0.6894838765719555 1101 50-51 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1105 68-69 -0.8941363222071814 1105 70-71 -0.8881652212821578 1105 72-73 -0.8726113929697803 1105 74-75 -0.7740227826992694 1105 76-77 -0.6649727563752172 1105 78-79 -0.5782301197846316 1105 80-81 -0.6239197835142036 1105 82-83 -0.7197424223733684 1105 84-85 -0.7375576992578097 1105 86-87 -0.8138500232509642 1105 88-89 -0.7406572759145291 1105 90-91 -0.7513028493270859 1105 92-93 -0.7809392928594825 1105 94-95 -0.753235024622235 1105 96-97 -0.9221116616534708 1105 98-99 -0.8582893982849846 1105 100 -0.8713845455526226 1106 1 -0.33748175899322064 1106 2 -0.37978687475322914 1106 3 -0.45838806620161066 1106 4 -0.21168131868262208 1106 5 -0.3305524986134927 1106 6 -0.347093665336331 1106 7 -0.3634618311132911 1106 8 -0.47599989584376345 1106 9 -0.5775124191498833 1106 10-11 -0.5035281391873241 1106 12-13 -0.44521358070315387 1106 14-15 -0.6850097460230486 1106 16-17 -0.6551445399833682 1106 18-19 -0.6422106184800711 1106 20-21 -0.6608730526523985 1106 22-23 -0.7556133285268913 1106 24-25 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0.46934866003460485 2311 3 0.41132079148839296 2311 4 0.3099780054928516 2311 5 0.34772451180536734 2311 6 0.4236957383941018 2311 7 0.3774994313726481 2311 8 0.2006028626120866 2311 9 0.4675720849300333 2311 10-11 0.37115128704830624 2311 12-13 0.49434070469759916 2311 14-15 0.5881588329707697 2311 16-17 0.49177142451914335 2311 18-19 0.5967581438474099 2311 20-21 0.729555126368794 2311 22-23 0.8042313644617494 2311 24-25 0.6449006457837143 2311 26-27 0.6493717254066738 2311 28-29 0.7119082574587381 2311 30-31 0.7676560621819206 2311 32-33 0.7287098249813795 2311 34-35 0.8232704513480016 2311 36-37 0.8658221388898184 2311 38-39 0.8278365501257454 2311 40-41 0.7688166640813705 2311 42-43 0.7917452620829621 2311 44-45 0.7784647579260948 2311 46-47 0.929603976474894 2311 48-49 -5.880954428126511 2311 50-51 -1.0366203867868933 2311 52-53 0.3415652720461111 2311 54-55 0.7115761498006563 2311 56-57 0.8660476097540979 2311 58-59 0.9551631093631556 2311 60-61 0.8155610271774165 2311 62-63 0.7656857427879586 2311 64-65 0.7305690779713885 2311 66-67 0.8865601803319052 2311 68-69 0.9152855107782827 2311 70-71 0.8822704136250508 2311 72-73 0.3719799554441252 2311 74-75 0.7377464581950406 2311 76-77 0.85756395786483 2311 78-79 0.6914072977141004 2311 80-81 0.7647700211152682 2311 82-83 0.8260530684974263 2311 84-85 0.8419084164602104 2311 86-87 0.88845296089751 2311 88-89 0.9805754011693928 2311 90-91 0.9310620930395501 2311 92-93 0.9658426875470525 2311 94-95 0.8923754847702199 2311 96-97 1.0536397815311247 2311 98-99 1.0538998704388085 2311 100 1.2665237299593102 2312 1 0.10705859024444209 2312 2 0.26370874061560556 2312 3 0.29915400623195154 2312 4 0.09396560910879259 2312 5 0.19845751669006972 2312 6 0.07673959259172847 2312 7 0.2888194256082883 2312 8 0.30544935640789106 2312 9 0.362378453350928 2312 10-11 0.29362735142904484 2312 12-13 0.3705752017118442 2312 14-15 0.39593194221446737 2312 16-17 0.543690664884096 2312 18-19 0.41668708005212096 2312 20-21 0.4636618451549168 2312 22-23 0.6970459125746657 2312 24-25 0.7733196714861705 2312 26-27 0.6070680139971429 2312 28-29 0.8389841414028822 2312 30-31 0.870726711663572 2312 32-33 0.8854132619831887 2312 34-35 0.43140161914201514 2312 36-37 0.8271783855685584 2312 38-39 1.0278742286483862 2312 40-41 0.7926832310725871 2312 42-43 0.747726502381866 2312 44-45 0.9164812989476445 2312 46-47 0.9903908046037913 2312 48-49 -4.452199860919134 2312 50-51 -0.720758216624084 2312 52-53 0.5891019072789874 2312 54-55 0.9318386385046082 2312 56-57 1.050201013881022 2312 58-59 1.0882609894583908 2312 60-61 1.1551105661784433 2312 62-63 0.969551319029172 2312 64-65 1.1240870961111185 2312 66-67 1.1810242146058485 2312 68-69 1.063666272514915 2312 70-71 1.15922162819448 2312 72-73 1.3330166477201786 2312 74-75 0.9011320160623413 2312 76-77 1.0655963133579398 2312 78-79 0.6505698212444102 2312 80-81 0.8514672133898245 2312 82-83 0.9036229764177257 2312 84-85 1.0198523700330675 2312 86-87 1.0370677122642888 2312 88-89 0.8999608134264712 2312 90-91 0.7528601316547778 2312 92-93 1.014895883316246 2312 94-95 1.1978946377690392 2312 96-97 1.2112272002070625 2312 98-99 1.2610401756498533 2312 100 0.708694328102105 2313 1 0.06712507979015925 2313 2 0.2213217787736994 2313 3 0.29772404116194195 2313 4 0.07534838804958355 2313 5 0.1071759793750715 2313 6 0.1875795531348743 2313 7 0.08799198807010811 2313 8 0.19040993908838288 2313 9 0.3945666285504359 2313 10-11 0.2272638264848439 2313 12-13 -0.05059670741878364 2313 14-15 0.062223758870558754 2313 16-17 0.09468743974083083 2313 18-19 0.4085832033567698 2313 20-21 0.4058622962598477 2313 22-23 0.47637502248256425 2313 24-25 0.41060138374764676 2313 26-27 0.39412417379345044 2313 28-29 0.37779495507204075 2313 30-31 0.5384650490592193 2313 32-33 0.3602551924039119 2313 34-35 0.29931814001995605 2313 36-37 -0.37337748956908 2313 38-39 0.018818030304551314 2313 40-41 0.24591618268654258 2313 42-43 0.382026711526926 2313 44-45 0.5024097952579609 2313 46-47 0.4724870834823136 2313 48-49 -5.245105237607568 2313 50-51 -1.2548069278516962 2313 52-53 0.1722321247858929 2313 54-55 0.3994448764832157 2313 56-57 0.38011844309689025 2313 58-59 0.47058650258530577 2313 60-61 0.7706760584303893 2313 62-63 0.81680624075792 2313 64-65 0.8985887457476878 2313 66-67 0.9119884069165067 2313 68-69 1.0194135855544602 2313 70-71 0.9624499590469284 2313 72-73 0.6665776832847214 2313 74-75 0.7671514301794318 2313 76-77 0.9384391343254173 2313 78-79 0.8468467971017972 2313 80-81 0.003548211632452336 2313 82-83 0.26794839960041017 2313 84-85 0.5238301785170805 2313 86-87 0.761600644703325 2313 88-89 0.7633897594579508 2313 90-91 0.8080225106501331 2313 92-93 0.9045032965002946 2313 94-95 0.8375629609910575 2313 96-97 0.9171142480396028 2313 98-99 1.1211487215382157 2313 100 1.1613557384672362 2314 1 0.1710686069276619 2314 2 0.21407135207424233 2314 3 0.2972510921210798 2314 4 0.18231898352780007 2314 5 0.2426675534433116 2314 6 0.2806718716693979 2314 7 0.10389807504946447 2314 8 0.21620030852831462 2314 9 0.40078072435233736 2314 10-11 0.33647470049612593 2314 12-13 0.22053849912175139 2314 14-15 0.30241994216815016 2314 16-17 0.48742178289106874 2314 18-19 0.5890623684382277 2314 20-21 0.593179652966981 2314 22-23 0.36628966071119606 2314 24-25 0.4593396271777195 2314 26-27 0.57138695721342 2314 28-29 0.6614558359022524 2314 30-31 0.44853945922212546 2314 32-33 0.43175254347600855 2314 34-35 0.25311043528990496 2314 36-37 0.49522776170894645 2314 38-39 0.4742035354313785 2314 40-41 0.25462350149697954 2314 42-43 0.3099893768737232 2314 44-45 0.46453497615397765 2314 46-47 0.6625127450093018 2314 48-49 -6.074973944717311 2314 50-51 -1.3165960608902765 2314 52-53 -0.7300707152664145 2314 54-55 -0.27648371902952107 2314 56-57 0.015717223809701864 2314 58-59 0.5231403666312389 2314 60-61 0.8163216575733188 2314 62-63 0.729890763108564 2314 64-65 0.8151657494119178 2314 66-67 0.8836677418755201 2314 68-69 0.8224090996492137 2314 70-71 0.7093045015091448 2314 72-73 0.824089161810214 2314 74-75 0.847221423234096 2314 76-77 0.8296896013793109 2314 78-79 0.5513119903132164 2314 80-81 0.7061412996967533 2314 82-83 0.6705190131651868 2314 84-85 0.710629369775436 2314 86-87 0.7070746402852421 2314 88-89 0.5924652954121683 2314 90-91 0.8514725614699685 2314 92-93 0.8617638847365896 2314 94-95 0.9538909219182479 2314 96-97 0.9891647100370342 2314 98-99 0.9717745555196124 2314 100 -0.18626691164698173 2315 1 0.03318002612486026 2315 2 0.15298151601631105 2315 3 0.24021936044133696 2315 4 0.11237784000961426 2315 5 0.23734331078203041 2315 6 0.2559678424248091 2315 7 0.3261174901091195 2315 8 0.2743807230307027 2315 9 0.45646785215699026 2315 10-11 0.19357470695800316 2315 12-13 0.30982985279653263 2315 14-15 0.1814239951710519 2315 16-17 0.38965644894102525 2315 18-19 0.44202161443003973 2315 20-21 0.28328489888980357 2315 22-23 0.43211409358472963 2315 24-25 0.5216067507748718 2315 26-27 0.590372654612203 2315 28-29 0.6511843741052772 2315 30-31 0.5675854269409442 2315 32-33 0.7480084763735988 2315 34-35 0.6276368480713899 2315 36-37 0.6956630149633298 2315 38-39 0.7704965949432818 2315 40-41 0.7628595949890951 2315 42-43 0.7750238458608649 2315 44-45 0.8242327281512019 2315 46-47 0.6407981819411859 2315 48-49 -4.892430607660707 2315 50-51 -0.8625533338246996 2315 52-53 0.30883874418582025 2315 54-55 0.13756888546939905 2315 56-57 0.5933591092771451 2315 58-59 0.7147869877279405 2315 60-61 0.4839868980934412 2315 62-63 0.371452347423606 2315 64-65 0.5925246121612631 2315 66-67 -0.03754466569662185 2315 68-69 0.5339149855403278 2315 70-71 0.6089424544657476 2315 72-73 0.6465863280605788 2315 74-75 0.7875434212450045 2315 76-77 0.8317318931187536 2315 78-79 0.7494122848871356 2315 80-81 0.6215331029332276 2315 82-83 0.6639763067484488 2315 84-85 0.5796270556477907 2315 86-87 0.606219632552861 2315 88-89 0.5885932127896005 2315 90-91 0.738621185018296 2315 92-93 0.7969183890745448 2315 94-95 0.3627459097305703 2315 96-97 0.49473594926380926 2315 98-99 0.5318273339546415 2315 100 0.7191259337485931 2316 1 0.17960962712010087 2316 2 0.21434907905550205 2316 3 0.17069583283913659 2316 4 0.025155163119698898 2316 5 0.18376604339292868 2316 6 0.11176224362745302 2316 7 0.22800600559007478 2316 8 0.2599527627086786 2316 9 0.3975250222665565 2316 10-11 0.40790989572248293 2316 12-13 0.3525098479983413 2316 14-15 0.45149902501466244 2316 16-17 0.3737740356809809 2316 18-19 0.10820072254615098 2316 20-21 0.45719650181592897 2316 22-23 0.5478826464451672 2316 24-25 0.5953613482391802 2316 26-27 0.566959686715613 2316 28-29 0.5633670015428223 2316 30-31 0.5226903777884573 2316 32-33 0.4617301055930625 2316 34-35 0.4447523297966498 2316 36-37 0.3401868811478579 2316 38-39 0.4015947044930428 2316 40-41 0.09249101426816253 2316 42-43 -0.03990923092380427 2316 44-45 0.21341231415208028 2316 46-47 0.21152730447122536 2316 48-49 -5.145621262401537 2316 50-51 -1.1458635468220066 2316 52-53 0.07829223361528648 2316 54-55 0.3660454488007403 2316 56-57 0.3532911799886165 2316 58-59 0.15359597118770552 2316 60-61 0.3107759187392176 2316 62-63 0.15152184386462153 2316 64-65 0.2542426562775688 2316 66-67 0.4019035128271966 2316 68-69 -0.024254183733425805 2316 70-71 -0.322508409499612 2316 72-73 -0.03936018367081573 2316 74-75 0.1732210986089413 2316 76-77 0.17472473839050195 2316 78-79 0.2529526468026191 2316 80-81 0.1975849814847166 2316 82-83 0.08860715527445251 2316 84-85 0.21787602393907335 2316 86-87 0.2936368008050607 2316 88-89 0.17709234505146654 2316 90-91 0.3376149183431174 2316 92-93 0.2602842650063728 2316 94-95 0.46073598830102114 2316 96-97 0.6846179968549393 2316 98-99 0.5493264778250442 2316 100 -0.30493807959348374 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 1.0 11 6.0 12 15.0 13 76.0 14 296.0 15 858.0 16 2205.0 17 4727.0 18 7844.0 19 11044.0 20 14673.0 21 19514.0 22 23735.0 23 24376.0 24 23194.0 25 24238.0 26 27663.0 27 32687.0 28 39949.0 29 48904.0 30 59404.0 31 73561.0 32 91882.0 33 119463.0 34 165433.0 35 232683.0 36 351830.0 37 525847.0 38 442683.0 39 63626.0 40 9.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.43941086892064 19.460662291309312 13.458439108604903 23.64148773116515 2 16.00747906995101 20.781594054895848 39.63631624559898 23.574610629554165 3 18.097577520375758 26.27246472141998 31.34308354831084 24.28687420989342 4 12.449832885941365 17.09522540660035 37.88912409863677 32.56581760882152 5 12.373119663214673 37.809250907536146 35.03689756249974 14.780731866749438 6 29.89361238533053 37.31204978075387 18.31550065654618 14.478837177369424 7 26.183242573463694 33.37680159643089 22.72813232550548 17.711823504599934 8 25.92693089618908 35.39915020628157 20.645547216586394 18.028371680942954 9 26.61976811117507 15.147520763095141 21.028574952717705 37.20413617301209 10-11 24.889723180979782 26.206014919823062 28.55788816114445 20.346373738052705 12-13 24.94924811566414 24.486211685307897 28.661289036195196 21.903251162832767 14-15 22.55990320766445 25.628632179258222 26.919484431990476 24.89198018108685 16-17 21.434567810891135 28.209037998798724 27.91677749598239 22.439616694327746 18-19 21.170798207221925 27.192584687057288 30.308321815339912 21.32829529038088 20-21 22.163615834167036 26.517668489882624 30.222923763070042 21.0957919128803 22-23 22.09338608742412 26.407550018130106 29.964031675476832 21.535032218968944 24-25 21.602113776216648 26.396508174561674 30.375501968406827 21.62587608081486 26-27 21.478489341076507 26.599989639955567 30.131305340931796 21.79021567803613 28-29 21.404490259305785 26.96531838841374 30.179902676309002 21.450288675971475 30-31 21.95331474364838 26.61413179216417 30.286070789492886 21.14648267469456 32-33 21.84700177001338 26.824131388031745 29.902042813355067 21.426824028599796 34-35 21.857756438024794 26.906880062369027 30.004484007143983 21.2308794924622 36-37 21.856068035720234 26.78193049958078 29.80458892412884 21.557412540570148 38-39 21.755114511099123 27.005095595878785 29.844866409200364 21.394923483821728 40-41 21.796241969829932 26.638487710147324 30.106053439290157 21.459216880732587 42-43 21.593247404009936 26.90758041021926 29.57814981566898 21.921022370101824 44-45 21.398990753473022 27.247230451476135 29.71856259974209 21.635216195308754 46-47 21.444608108677617 26.888459684291348 29.333603327923164 22.333328879107867 48-49 21.781983535594225 27.133019448173577 29.092152232915332 21.99284478331687 50-51 21.676407652656753 27.27009381609808 28.96421945466926 22.08927907657591 52-53 21.486441783644615 27.59215707559554 28.717188968392154 22.20421217236769 54-55 21.53335217782346 27.336687652576124 28.848844918411924 22.28111525118849 56-57 21.72033939071005 27.290428867863014 28.536584162506923 22.452647578920015 58-59 21.36128403333428 27.41180761044169 28.939902829154214 22.287005527069816 60-61 21.680201259226916 27.51567866607792 28.703162374819406 22.100957699875753 62-63 21.70937159392213 27.44915885936915 28.592203131854554 22.24926641485417 64-65 21.326075965919102 27.23564657465019 28.623870979282 22.814406480148705 66-67 21.442030699003773 27.15710364255092 28.490916754911737 22.90994890353357 68-69 21.13946934345862 27.264567886968077 28.615307934844697 22.980654834728604 70-71 21.28343173999258 27.024615235308293 28.590403767498746 23.101549257200382 72-73 21.35028726125899 27.477938051574412 28.170477935378706 23.00129675178789 74-75 21.48597159823746 27.521109723691527 28.042927045074507 22.949991632996504 76-77 21.41676923769595 27.383761342519257 28.083766214942962 23.115703204841832 78-79 21.24901812407787 27.369715523743565 28.178281737179802 23.20298461499877 80-81 21.22411978490026 27.557865611998224 27.92247035800622 23.295544245095297 82-83 21.53612637260973 27.37818281397638 28.127901363707757 22.95778944970613 84-85 21.609374588783325 27.563915407795353 27.654670927967995 23.172039075453323 86-87 21.168838912499513 27.702600732374787 27.942462728455908 23.186097626669792 88-89 21.274404733019193 27.253374118583746 27.962763581809163 23.509457566587898 90-91 21.448877450171093 27.207736842419074 27.887412149324614 23.455973558085223 92-93 21.445024102785922 27.348808676016123 27.724803090682727 23.481364130515228 94-95 21.105899006502373 27.205685399065192 27.8745268557236 23.81388873870883 96-97 21.102415780688556 27.124497397567776 27.948498154040962 23.824588667702706 98-99 21.062613407366868 27.19700368125504 27.656286813064362 24.08409609831373 100 20.99146478782879 27.17054878856247 27.72574472337671 24.112241700232033 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1749.0 1 1284.5 2 1727.5 3 2341.0 4 1526.5 5 1146.0 6 1678.0 7 3103.0 8 4683.0 9 5268.0 10 4316.0 11 3142.5 12 3036.0 13 3286.0 14 3620.5 15 3988.0 16 4359.5 17 4672.0 18 5019.5 19 5350.0 20 5868.5 21 6607.0 22 7570.5 23 8923.5 24 10561.5 25 12507.0 26 15058.0 27 17795.5 28 20386.0 29 23896.0 30 28538.5 31 33127.0 32 38715.5 33 44913.5 34 50626.0 35 55981.5 36 61873.0 37 68067.5 38 71081.5 39 71963.5 40 71826.0 41 71022.0 42 71667.0 43 74175.5 44 77519.5 45 82229.5 46 86755.0 47 89387.0 48 92285.0 49 94883.5 50 94956.5 51 93366.0 52 91191.5 53 87427.5 54 82431.0 55 75908.0 56 68093.0 57 59010.5 58 50862.0 59 44075.5 60 35672.0 61 28126.0 62 22661.5 63 17725.0 64 13167.5 65 9586.0 66 6991.0 67 5239.0 68 4015.5 69 2989.5 70 2279.5 71 1767.5 72 1358.0 73 1012.5 74 780.5 75 575.0 76 366.0 77 207.5 78 135.0 79 86.5 80 46.5 81 26.5 82 18.0 83 12.5 84 6.5 85 4.5 86 5.0 87 4.0 88 1.0 89 0.5 90 1.0 91 0.5 92 1.0 93 2.0 94 1.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03194341780592709 2 0.0015622263534430234 3 4.1111219827447986E-5 4 0.022775615784406185 5 6.577795172391678E-4 6 0.0 7 0.0 8 0.0029188966077488073 9 0.009291135681003246 10-11 0.0010072248857724757 12-13 1.6444487930979194E-4 14-15 8.222243965489597E-5 16-17 2.877785387921359E-4 18-19 0.0 20-21 1.0277804956861996E-4 22-23 3.288897586195839E-4 24-25 8.222243965489597E-5 26-27 1.6444487930979194E-4 28-29 0.0011305585452548196 30-31 0.003021674657317427 32-33 0.009907803978414965 34-35 0.01847949331243787 36-37 0.02166561284906509 38-39 0.021460056749927847 40-41 0.004707234670242795 42-43 0.004810012719811414 44-45 0.008859467872815042 46-47 0.011449474721944264 48-49 1.2096565322028296 50-51 0.017842269405112427 52-53 0.018520604532265318 54-55 0.009805025928846346 56-57 0.007605575668077878 58-59 0.008838912262901316 60-61 0.005755570775842718 62-63 0.007934465426697462 64-65 0.020822832842602407 66-67 0.030874526090413437 68-69 0.031203415849033023 70-71 0.02795562948266463 72-73 0.023865063109833558 74-75 0.010401138616344341 76-77 0.015272818165896929 78-79 0.009023912752124833 80-81 0.002713340508611567 82-83 0.015622263534430237 84-85 0.025406733853362857 86-87 0.025118955314570722 88-89 0.034040090017126934 90-91 0.02176839089863371 92-93 0.014060037180987212 94-95 0.006598350782305401 96-97 0.003535564905160527 98-99 0.015416707435292996 100 0.03502675929298569 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2432426.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.46070092367336 #Duplication Level Percentage of deduplicated Percentage of total 1 76.44839603261092 50.0436558878528 2 15.773578526351518 20.650990128191456 3 4.170333483257278 8.189788586984568 4 1.4569893785089485 3.8150218382217203 5 0.6894033992916152 2.2564414868396097 6 0.3619582781837302 1.4216425557019758 7 0.2407390575730244 1.1031263213904312 8 0.1529111971582581 0.8007739316046067 9 0.12161231405854382 0.7164744587299957 >10 0.5402815522999056 6.035564637794791 >50 0.02958766365777374 1.3261957698123885 >100 0.013038072960551591 1.5105121914925583 >500 6.199645171493929E-4 0.2546377355141584 >1k 3.444247317494069E-4 0.5132887096433312 >5k 6.888494634988138E-5 0.34049398634075323 >10k+ 1.3776989269976276E-4 1.0213917738848366 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11381 0.46788679285618556 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11270 0.46332344745533877 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7551 0.31043082091705976 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3235 0.13299479614179424 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 4.1111219827447986E-5 0.0 12-13 0.0 0.0 0.0 4.1111219827447986E-5 4.1111219827447986E-5 14-15 0.0 0.0 0.0 8.222243965489597E-5 4.1111219827447986E-5 16-17 0.0 0.0 0.0 8.222243965489597E-5 4.1111219827447986E-5 18-19 0.0 0.0 0.0 8.222243965489597E-5 4.1111219827447986E-5 20-21 0.0 0.0 0.0 1.2333365948234397E-4 4.1111219827447986E-5 22-23 0.0 0.0 0.0 2.4666731896468794E-4 1.2333365948234394E-4 24-25 0.0 0.0 0.0 0.0010277804956861996 1.6444487930979194E-4 26-27 0.0 0.0 0.0 0.0024666731896468793 2.2611170905096394E-4 28-29 0.0 2.0555609913723993E-5 0.0 0.008674467383591525 2.4666731896468794E-4 30-31 0.0 4.1111219827447986E-5 0.0 0.02803785192231953 2.4666731896468794E-4 32-33 0.0 4.1111219827447986E-5 0.0 0.06978629565709296 2.4666731896468794E-4 34-35 0.0 4.1111219827447986E-5 0.0 0.13552313616118228 3.288897586195839E-4 36-37 0.0 4.1111219827447986E-5 0.0 0.23982230086341783 3.288897586195839E-4 38-39 0.0 4.1111219827447986E-5 0.0 0.38572602003103074 3.288897586195839E-4 40-41 0.0 4.1111219827447986E-5 0.0 0.5417019880563684 3.288897586195839E-4 42-43 0.0 4.1111219827447986E-5 0.0 0.6901340472433694 3.288897586195839E-4 44-45 0.0 4.1111219827447986E-5 0.0 0.8737161993828384 3.288897586195839E-4 46-47 0.0 4.1111219827447986E-5 0.0 1.093517336190289 3.288897586195839E-4 48-49 0.0 8.222243965489597E-5 0.0 1.3179640408382414 3.288897586195839E-4 50-51 0.0 1.2333365948234397E-4 0.0 1.5530791070314165 3.494453685333079E-4 52-53 0.0 1.2333365948234397E-4 0.0 1.7902086229961363 3.700009784470319E-4 54-55 0.0 1.2333365948234397E-4 0.0 2.0626526767926343 3.700009784470319E-4 56-57 0.0 1.2333365948234397E-4 0.0 2.3717062718454747 3.700009784470319E-4 58-59 0.0 1.2333365948234397E-4 0.0 2.7071532700275363 3.9055658836075586E-4 60-61 0.0 2.2611170905096392E-4 0.0 3.0316235725156693 4.111121982744799E-4 62-63 0.0 3.288897586195839E-4 0.0 3.373895033189088 4.111121982744799E-4 64-65 0.0 3.288897586195839E-4 0.0 3.7213465075607646 4.111121982744799E-4 66-67 0.0 3.288897586195839E-4 0.0 4.114616436430132 4.111121982744799E-4 68-69 0.0 3.288897586195839E-4 0.0 4.540508118232579 4.111121982744799E-4 70-71 0.0 3.288897586195839E-4 0.0 4.983666512362555 4.111121982744799E-4 72-73 0.0 3.288897586195839E-4 0.0 5.4171637698330795 4.522234181019279E-4 74-75 0.0 3.288897586195839E-4 0.0 5.85382659123032 4.522234181019279E-4 76-77 0.0 3.288897586195839E-4 0.0 6.325536727530457 4.522234181019279E-4 78-79 0.0 3.288897586195839E-4 0.0 6.839961421231314 4.522234181019279E-4 80-81 0.0 3.288897586195839E-4 0.0 7.364910587207998 4.522234181019279E-4 82-83 0.0 3.288897586195839E-4 0.0 7.884576961436854 4.522234181019279E-4 84-85 0.0 3.288897586195839E-4 0.0 8.40165332881658 4.522234181019279E-4 86-87 0.0 3.288897586195839E-4 0.0 8.938565859763052 4.522234181019279E-4 88 0.0 3.288897586195839E-4 0.0 9.363738095218519 4.522234181019279E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5235 0.0 59.3471 1 TACCTGG 1825 0.0 39.588875 2 GTATCAA 8915 0.0 36.007496 1 GTGGTAT 2005 0.0 31.599308 1 ACCTGGG 2370 0.0 29.89431 3 GTACCTG 2525 0.0 29.366615 1 ATCAACG 11320 0.0 27.687899 3 TGGTATC 2240 0.0 27.646568 2 GTACATG 9810 0.0 27.412193 1 TAACGCA 275 0.0 27.29903 4 CAACGCA 11505 0.0 27.280094 5 TATCAAC 11720 0.0 26.98035 2 TACATGG 9960 0.0 26.566544 2 TCAACGC 11915 0.0 26.344627 4 AACGCAG 12085 0.0 26.164932 6 ACATGGG 9975 0.0 25.68264 3 CATGGGG 6530 0.0 24.214565 4 ATGGGGG 4330 0.0 23.836426 5 ATAACGC 320 0.0 23.460106 3 ACGCAGA 13680 0.0 22.977093 7 >>END_MODULE