FastQCFastQC Report
Mon 6 Jun 2016
ERR1211338_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211338_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6919281
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT297130.42942323053508014No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT236390.34163954318374984No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139370.20142266226794375No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA131350.052.6762471
GTATCAA240450.041.0405271
ATCAACG298450.032.433073
CAACGCA301200.032.1208885
TATCAAC305000.031.8439772
TCAACGC314350.030.9268974
AACGCAG318450.030.483576
TACCTGG47850.029.3897782
GTACATG306650.026.9808181
ACGCAGA357600.026.897367
CGCAGAG363900.026.3145358
TACATGG315800.025.7507552
GTGGTAT66000.025.5848431
ACATGGG316350.024.6417183
GTACTAG27000.024.3891431
GCAGAGT392000.023.9266879
TGGTATC71350.023.0717832
ACCTGGG61050.022.5514453
CATGGGG197650.022.370834
GTACCTG65700.022.3368261
Produced by FastQC (version 0.11.5)