FastQCFastQC Report
Mon 6 Jun 2016
ERR1211338_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211338_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6919281
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT257970.3728277547912854No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT217420.31422339980122216No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133600.19308364554062768No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA132750.051.8533821
GTATCAA237000.039.7752651
TACCTGG51700.032.578572
ATCAACG295950.031.1852113
CAACGCA296800.031.1058645
TATCAAC308200.030.2173272
TCAACGC308700.029.988394
AACGCAG316950.029.4835386
GTACATG299850.026.1958751
ACCTGGG62350.025.9624273
ACGCAGA357250.025.8949897
GTACCTG67700.025.5059011
CGCAGAG367150.025.1967458
TACATGG308750.024.9057792
GTGGTAT66350.024.3275851
ACATGGG308800.024.2655583
GCAGAGT400300.022.9453989
TGGTATC69300.022.273622
GTACTAG24150.021.7611791
CATGGGG198000.021.706664
Produced by FastQC (version 0.11.5)