##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211338_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6919281 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.11519679573644 33.0 31.0 34.0 30.0 34.0 2 32.24116161780393 33.0 31.0 34.0 30.0 34.0 3 32.305449222253 34.0 31.0 34.0 30.0 34.0 4 35.94840995762421 37.0 35.0 37.0 35.0 37.0 5 35.85299686484766 37.0 35.0 37.0 35.0 37.0 6 35.874900874816326 37.0 35.0 37.0 35.0 37.0 7 35.79106730887212 37.0 35.0 37.0 33.0 37.0 8 35.775840004185405 37.0 35.0 37.0 33.0 37.0 9 37.392330792751444 39.0 37.0 39.0 34.0 39.0 10-11 37.341048224519284 39.0 37.0 39.0 34.0 39.0 12-13 37.27218767383489 39.0 37.0 39.0 34.0 39.0 14-15 38.5003482298233 40.0 38.0 41.0 34.0 41.0 16-17 38.48670078581864 40.0 38.0 41.0 34.0 41.0 18-19 38.50642393335377 40.0 38.0 41.0 34.0 41.0 20-21 38.479164598171394 40.0 38.0 41.0 34.0 41.0 22-23 38.367167773645846 40.0 38.0 41.0 34.0 41.0 24-25 38.274652669836655 40.0 38.0 41.0 34.0 41.0 26-27 38.17210502073843 40.0 38.0 41.0 33.5 41.0 28-29 38.06943271996035 40.0 37.5 41.0 33.0 41.0 30-31 38.064311017286336 40.0 37.0 41.0 33.0 41.0 32-33 37.98685622104378 40.0 37.0 41.0 33.0 41.0 34-35 37.84875075893001 40.0 37.0 41.0 33.0 41.0 36-37 37.69580488203904 40.0 37.0 41.0 33.0 41.0 38-39 37.58000238753131 40.0 37.0 41.0 32.5 41.0 40-41 37.4694308556048 40.0 36.0 41.0 32.0 41.0 42-43 37.35598677087981 40.0 36.0 41.0 32.0 41.0 44-45 37.18697441251483 40.0 36.0 41.0 31.0 41.0 46-47 36.93271865964108 39.0 36.0 41.0 31.0 41.0 48-49 35.37532606350284 39.0 35.0 41.0 24.0 41.0 50-51 36.09740932620022 39.0 35.0 40.0 28.5 41.0 52-53 36.19035792880792 39.0 35.0 40.0 29.0 41.0 54-55 36.01858654100043 38.5 35.0 40.0 29.0 41.0 56-57 35.80637063301808 38.0 35.0 40.0 28.0 41.0 58-59 35.569485976938935 38.0 34.0 40.0 28.0 41.0 60-61 35.31990599890365 37.5 34.0 40.0 28.0 41.0 62-63 35.00282522129106 37.0 34.0 40.0 27.0 41.0 64-65 34.67396612451496 36.5 34.0 39.5 26.5 41.0 66-67 34.33739285917135 36.0 33.0 39.0 26.0 40.5 68-69 33.97875877565892 35.5 33.0 39.0 26.0 40.0 70-71 33.628776458131995 35.0 33.0 38.0 26.0 40.0 72-73 33.25762156501521 35.0 32.0 37.5 25.0 39.5 74-75 32.894748674031305 35.0 32.0 37.0 25.0 39.0 76-77 31.960788555920768 34.0 30.5 36.0 24.0 38.5 78-79 32.39702195936255 35.0 31.5 36.0 25.0 38.0 80-81 32.27091615443859 35.0 32.0 36.0 25.0 37.0 82-83 32.00228535305908 34.0 31.0 35.0 24.5 37.0 84-85 31.71931231005071 34.0 31.0 35.0 24.0 36.5 86-87 31.462089052316273 34.0 31.0 35.0 23.5 36.0 88-89 31.234290889472476 34.0 31.0 35.0 23.0 36.0 90-91 31.02024321602201 34.0 31.0 35.0 21.5 35.0 92-93 30.833607350243472 34.0 30.0 35.0 20.5 35.0 94-95 30.641125790381977 34.0 30.0 35.0 20.0 35.0 96-97 30.457309075899648 34.0 30.0 35.0 20.0 35.0 98-99 30.242925095830046 34.0 29.0 35.0 19.0 35.0 100 27.818388933763494 31.0 25.0 34.0 13.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.2771010200099653 1101 2 -0.2687904137580368 1101 3 -0.29261527489607175 1101 4 -0.19088771779644986 1101 5 -0.18882500297748805 1101 6 -0.210382170044646 1101 7 -0.22330209024016057 1101 8 -0.20565232743830109 1101 9 -0.3532460565490112 1101 10-11 -0.33320438356307136 1101 12-13 -0.4198593327261193 1101 14-15 -0.45426250469770935 1101 16-17 -0.5136958944993211 1101 18-19 -0.5291610940474882 1101 20-21 -0.5501127460995505 1101 22-23 -0.6726411020683614 1101 24-25 -0.5445882988400612 1101 26-27 -0.5373941400471622 1101 28-29 -0.5429295734941562 1101 30-31 -0.5189586982026384 1101 32-33 -0.48595769856105164 1101 34-35 -0.5085770544760635 1101 36-37 -0.5295590167734687 1101 38-39 -0.5335742946633886 1101 40-41 -0.549879537338505 1101 42-43 -0.5626039488112298 1101 44-45 -0.5007673948419082 1101 46-47 -0.5663076554565905 1101 48-49 0.6505791910542271 1101 50-51 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1.0716706579599098 2310 98-99 1.1488263656010922 2310 100 1.3081555719253224 2311 1 0.3799098012754243 2311 2 0.4411638493190253 2311 3 0.4135830677733594 2311 4 0.26784855614025105 2311 5 0.37648796737559564 2311 6 0.42597783983612914 2311 7 0.42708493219563337 2311 8 0.20746884548900368 2311 9 0.4451874423332711 2311 10-11 0.4105433437828907 2311 12-13 0.5072745839409265 2311 14-15 0.48705062522061837 2311 16-17 0.5140666171803971 2311 18-19 0.6776365388634602 2311 20-21 0.7238066212856182 2311 22-23 0.7142469306916084 2311 24-25 0.6305420767059502 2311 26-27 0.6592505403697331 2311 28-29 0.7977471365773354 2311 30-31 0.6572355479359615 2311 32-33 0.64461362173396 2311 34-35 0.7730697202346875 2311 36-37 0.8767338376186444 2311 38-39 0.8859850290868252 2311 40-41 0.7931615000810837 2311 42-43 0.8496172147569538 2311 44-45 0.8812991592085169 2311 46-47 0.9143901663243739 2311 48-49 -5.885364721761775 2311 50-51 -0.9558495516722516 2311 52-53 0.513578204950619 2311 54-55 0.9079521927823109 2311 56-57 1.0124225267723261 2311 58-59 1.0504203286447122 2311 60-61 0.9307364267017277 2311 62-63 0.8172575809398168 2311 64-65 0.8932437301980656 2311 66-67 0.9319641115253603 2311 68-69 0.9831143106230158 2311 70-71 0.9202529307997835 2311 72-73 0.52614116543851 2311 74-75 0.8497055782997975 2311 76-77 0.8984307548809092 2311 78-79 0.8368740363971696 2311 80-81 0.8697066402496532 2311 82-83 0.8769555758396628 2311 84-85 0.9131083608915382 2311 86-87 0.8984988942114711 2311 88-89 0.9211388802923146 2311 90-91 1.0275622926284562 2311 92-93 0.9731161637368793 2311 94-95 1.0456083693946248 2311 96-97 1.0358887870576794 2311 98-99 1.0793284581424878 2311 100 1.2446466470748518 2312 1 0.056247049965755025 2312 2 0.2201445885409825 2312 3 0.18400437676449855 2312 4 0.008956462677886634 2312 5 0.18430303664688807 2312 6 0.12731046678175773 2312 7 0.29710939775034717 2312 8 0.315243852813758 2312 9 0.2836462884918731 2312 10-11 0.22949815700337695 2312 12-13 0.255637601694076 2312 14-15 0.3060560667158043 2312 16-17 0.4377391321049231 2312 18-19 0.2979231763162744 2312 20-21 0.2642778615014265 2312 22-23 0.6289970087718686 2312 24-25 0.7030535774169664 2312 26-27 0.5883355872707199 2312 28-29 0.7033602158240555 2312 30-31 0.7382315808121476 2312 32-33 0.7390699198511967 2312 34-35 0.3968397156796968 2312 36-37 0.7001344784623527 2312 38-39 0.7809611004490264 2312 40-41 0.5658959666981573 2312 42-43 0.5787928863515432 2312 44-45 0.6962441164202104 2312 46-47 0.6950396564007448 2312 48-49 -4.63372031851236 2312 50-51 -0.7781621392079288 2312 52-53 0.49131363277592044 2312 54-55 0.7374216738273489 2312 56-57 0.853548821997336 2312 58-59 0.8675021987668501 2312 60-61 0.8679678013572598 2312 62-63 0.7282146666580402 2312 64-65 0.849583482043343 2312 66-67 0.8843768834148023 2312 68-69 0.8108616521880876 2312 70-71 0.8196776310908049 2312 72-73 0.9560927659218734 2312 74-75 0.6562213659912999 2312 76-77 0.8350176572373051 2312 78-79 0.49034109029928885 2312 80-81 0.7099788157575091 2312 82-83 0.7254133465262562 2312 84-85 0.7584951634493784 2312 86-87 0.8576845595866658 2312 88-89 0.8310570829877371 2312 90-91 0.6927093492958356 2312 92-93 0.8829205695353188 2312 94-95 0.9726983461020176 2312 96-97 1.103257786908852 2312 98-99 1.023706905355855 2312 100 0.6150152718623296 2313 1 0.06674711824499013 2313 2 0.23280736874062313 2313 3 0.3158476328486799 2313 4 0.05069955524586334 2313 5 0.15235554582927335 2313 6 0.2669837165668625 2313 7 0.15828438152423274 2313 8 0.24515216468319068 2313 9 0.4568205373164105 2313 10-11 0.2636950644331364 2313 12-13 0.03188844718403061 2313 14-15 0.09247735880852304 2313 16-17 0.044004835460832226 2313 18-19 0.44113716803719427 2313 20-21 0.42568104129195206 2313 22-23 0.4850472353314075 2313 24-25 0.2522736384501485 2313 26-27 0.4273723071157818 2313 28-29 0.37141716651063206 2313 30-31 0.5510633046470446 2313 32-33 0.4040630811792525 2313 34-35 0.3455874563594037 2313 36-37 -0.4193378388363769 2313 38-39 0.08369498043540347 2313 40-41 0.28470722072631816 2313 42-43 0.35723417891912845 2313 44-45 0.36944092026336506 2313 46-47 0.3099135945349545 2313 48-49 -5.392939837934168 2313 50-51 -1.52705697966217 2313 52-53 -0.06780960071801445 2313 54-55 0.09878894499383506 2313 56-57 0.118912422231503 2313 58-59 0.24136819894065553 2313 60-61 0.5749192209180904 2313 62-63 0.6370248314832665 2313 64-65 0.7377457193305048 2313 66-67 0.722824069541673 2313 68-69 0.757553920322863 2313 70-71 0.7177509928768089 2313 72-73 0.4704566923350768 2313 74-75 0.6629346317089286 2313 76-77 0.6693210718731741 2313 78-79 0.6123599993084312 2313 80-81 -0.3504925791209672 2313 82-83 0.07755567165144228 2313 84-85 0.42189735954692864 2313 86-87 0.5666241745408982 2313 88-89 0.5493633906785043 2313 90-91 0.644840795321759 2313 92-93 0.7048383373477982 2313 94-95 0.6795883070757718 2313 96-97 0.7169820413571983 2313 98-99 0.7704581947456965 2313 100 0.8492853041008921 2314 1 0.10289431557012563 2314 2 0.2039962566828919 2314 3 0.28263944337830793 2314 4 0.06406359954610963 2314 5 0.18862555328129105 2314 6 0.32154382590692165 2314 7 0.11332553416365698 2314 8 0.2511843413113297 2314 9 0.41107006293847803 2314 10-11 0.3503922580545975 2314 12-13 0.25370916214390604 2314 14-15 0.28036751956796024 2314 16-17 0.5342903935303909 2314 18-19 0.5876454162008784 2314 20-21 0.6455534381023114 2314 22-23 0.4026243870993298 2314 24-25 0.46349129539100886 2314 26-27 0.52230295750865 2314 28-29 0.637388176759984 2314 30-31 0.601363752077674 2314 32-33 0.545331123388074 2314 34-35 0.42138062748527005 2314 36-37 0.5090030066590145 2314 38-39 0.5299249282300096 2314 40-41 0.323744236248416 2314 42-43 0.3833389850376818 2314 44-45 0.5805889009677685 2314 46-47 0.7028739702895805 2314 48-49 -6.475540848326293 2314 50-51 -1.556479752383673 2314 52-53 -0.6849466323575655 2314 54-55 -0.3144801596741047 2314 56-57 0.07066892727085872 2314 58-59 0.4937984476466326 2314 60-61 0.7075642723775957 2314 62-63 0.5710559081625561 2314 64-65 0.6818499834724463 2314 66-67 0.750184383488218 2314 68-69 0.8526482290341519 2314 70-71 0.5563890613731175 2314 72-73 0.7737447593114837 2314 74-75 0.8191587979614141 2314 76-77 0.7649508433597951 2314 78-79 0.578731893553261 2314 80-81 0.6084559568751615 2314 82-83 0.5249983343024631 2314 84-85 0.5571180105430749 2314 86-87 0.6599380488267599 2314 88-89 0.6250154363793676 2314 90-91 0.8000722802267823 2314 92-93 0.765300924450127 2314 94-95 0.8631210904909139 2314 96-97 0.9155274333515955 2314 98-99 1.029350307127057 2314 100 -0.354662640026028 2315 1 0.14326839275719294 2315 2 0.19991455815397075 2315 3 0.2911194387261986 2315 4 0.14397412765506346 2315 5 0.22454376200312964 2315 6 0.2795005828579562 2315 7 0.3280439467263463 2315 8 0.24661776870276242 2315 9 0.4281794897356974 2315 10-11 0.1735383618041908 2315 12-13 0.39035911808344537 2315 14-15 0.24810212806901433 2315 16-17 0.36018361365596263 2315 18-19 0.3984649865699552 2315 20-21 0.3699731307051053 2315 22-23 0.4503643475973078 2315 24-25 0.5027326115380362 2315 26-27 0.5136255496874327 2315 28-29 0.5453690835979259 2315 30-31 0.5573291311073376 2315 32-33 0.6328317576067235 2315 34-35 0.6425321604821335 2315 36-37 0.5572173764381887 2315 38-39 0.6342036319288908 2315 40-41 0.6247679620211528 2315 42-43 0.6938723446947108 2315 44-45 0.7078810236197199 2315 46-47 0.6280094047652796 2315 48-49 -4.807752602394011 2315 50-51 -0.9002845200054992 2315 52-53 0.28996020729130123 2315 54-55 0.146828704092556 2315 56-57 0.48514174657390186 2315 58-59 0.5842311933006883 2315 60-61 0.5928180701307468 2315 62-63 0.33890115826223877 2315 64-65 0.5897342587276313 2315 66-67 -0.11000555077301044 2315 68-69 0.6396237561419369 2315 70-71 0.5981510057582966 2315 72-73 0.6284658600764743 2315 74-75 0.8358458191187026 2315 76-77 0.7907570058202715 2315 78-79 0.7191196665071686 2315 80-81 0.6085081130645023 2315 82-83 0.5514753803467549 2315 84-85 0.6283213686999538 2315 86-87 0.6662534021413862 2315 88-89 0.6324617735488864 2315 90-91 0.672943773020517 2315 92-93 0.7341587215990693 2315 94-95 0.3829298098403022 2315 96-97 0.5044838949390282 2315 98-99 0.5044346364696999 2315 100 0.4522350716947692 2316 1 0.2211418876402007 2316 2 0.2258781004424648 2316 3 0.21596086814206217 2316 4 -0.04412878107231677 2316 5 0.13683180359306846 2316 6 0.11459966267100441 2316 7 0.232116144342136 2316 8 0.27637961617676865 2316 9 0.45915189070583295 2316 10-11 0.4115145349779894 2316 12-13 0.3196278324824249 2316 14-15 0.4516574530120039 2316 16-17 0.3957523275554493 2316 18-19 0.09373529240065892 2316 20-21 0.43914564955547064 2316 22-23 0.5625759318144148 2316 24-25 0.5892920672228215 2316 26-27 0.606001785504894 2316 28-29 0.6885111829915687 2316 30-31 0.5959872896710934 2316 32-33 0.5689040543291313 2316 34-35 0.4983924390990353 2316 36-37 0.42721691623506786 2316 38-39 0.4762788702355252 2316 40-41 0.1304599756360929 2316 42-43 0.09096729498424594 2316 44-45 0.30898406288519453 2316 46-47 0.3617703564421859 2316 48-49 -4.77810607219762 2316 50-51 -0.9462461188419056 2316 52-53 0.20304859418890686 2316 54-55 0.4772580623348901 2316 56-57 0.42850654194280935 2316 58-59 0.25337107610513243 2316 60-61 0.4078019791636649 2316 62-63 0.3234640770744832 2316 64-65 0.38263619970663854 2316 66-67 0.4576026581785726 2316 68-69 0.16433990736486237 2316 70-71 -0.1857772027985689 2316 72-73 -0.03096723181113248 2316 74-75 0.19854315975990033 2316 76-77 0.10416004403802148 2316 78-79 0.23424757251779482 2316 80-81 0.10671147064068975 2316 82-83 0.07309179248749231 2316 84-85 0.22070209690392417 2316 86-87 0.29563994346305833 2316 88-89 0.16532508853176253 2316 90-91 0.09457722729600349 2316 92-93 0.2815400135594821 2316 94-95 0.46654321174704805 2316 96-97 0.4798391498190675 2316 98-99 0.4909436266640519 2316 100 -0.2814699916481871 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 2.0 10 4.0 11 9.0 12 36.0 13 172.0 14 609.0 15 1932.0 16 4493.0 17 9389.0 18 15723.0 19 23255.0 20 32702.0 21 45511.0 22 56251.0 23 58821.0 24 59455.0 25 63480.0 26 74062.0 27 90096.0 28 111134.0 29 136349.0 30 167582.0 31 207490.0 32 261887.0 33 340179.0 34 474186.0 35 668714.0 36 1039475.0 37 1542080.0 38 1267022.0 39 167168.0 40 13.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.291785060073614 20.07811471097745 14.230238173682277 23.399862055266656 2 15.401666157452198 20.594670966999722 40.43276099939386 23.570901876154224 3 18.51595455940766 26.32658387958509 30.063159787237854 25.094301773769395 4 12.655114432400147 17.04742377485546 38.31181202799273 31.98564976475166 5 13.10609566418075 37.8849516262321 34.644685273511016 14.364267436076128 6 30.43104635613046 37.44484071244995 17.99638372726407 14.12772920415552 7 27.382433921222137 32.56051678216762 22.28399808708393 17.77305120952631 8 26.06600726463782 35.48331289684226 20.01585201089869 18.434827827621234 9 26.513420118151615 16.026567925616977 20.794707806586565 36.66530414964484 10-11 25.051149267846277 26.454134681136626 27.850211790812228 20.64450426020487 12-13 25.438559282687905 24.20595913546151 28.25458387948276 22.100897702367828 14-15 22.93380328650229 25.49624086121211 26.109448524273727 25.460507328011868 16-17 21.90303993446699 28.406516245015183 27.009445083803023 22.680998736714802 18-19 21.672085582302554 27.42325394791742 29.353837486871832 21.550822982908198 20-21 22.64975765870116 26.602038466338325 29.240654777118486 21.507549097842034 22-23 22.654007901274465 26.395367079169624 28.942820287159176 22.007804732396735 24-25 22.19524606537762 26.63019514027401 29.27393880200524 21.90061999234313 26-27 22.106879390271466 26.775185727524093 29.091352910333395 22.026581971871046 28-29 21.862519121584626 26.79913073885102 29.332375324088684 22.00597481547567 30-31 22.309063928450257 26.626271254111355 29.237173462887707 21.82749135455068 32-33 22.309837197354405 26.890328761280387 28.89053357175373 21.90930046961148 34-35 22.37930549238456 26.861492162817274 29.076381931847887 21.682820412950278 36-37 22.404789888373216 26.62551112252781 28.912378096920243 22.057320892178726 38-39 22.281258404506698 26.843726064886457 29.01215655314272 21.86285897746412 40-41 22.23508866305812 26.465031456376238 29.30638867446159 21.99349120610405 42-43 22.088558426983344 26.566653682972884 28.96849731931287 22.3762905707309 44-45 22.040185790862495 26.95473582631383 28.847073185414562 22.158005197409114 46-47 22.136104100488126 26.782457217051924 28.520515160218253 22.560923522241698 48-49 22.407123630079578 26.89442576487498 28.34710969866066 22.351340906384785 50-51 22.26099122569525 26.919325861179487 28.211620890942974 22.608062022182292 52-53 22.09699873677126 27.21220782866461 28.07024644596759 22.620546988596537 54-55 22.14788168493912 27.091380467642935 28.06836833746422 22.692369509953732 56-57 22.304725172240474 27.06210702306091 27.874971499517432 22.75819630518118 58-59 22.02066530884828 27.047450339814734 28.235947334877316 22.695937016459673 60-61 22.221855273044035 27.308999399688812 27.938108472698342 22.531036854568814 62-63 22.174463847463556 27.118976865729888 27.98399708617465 22.722562200631906 64-65 21.885391010241634 26.988370380253425 27.995498370251603 23.13074023925334 66-67 22.05908704294375 26.95280992015708 27.821498063650697 23.166604973248475 68-69 21.811266070796872 27.050953387334303 27.92782223887616 23.209958302992664 70-71 21.89490701374519 26.69464316020559 28.086625262342874 23.323824563706346 72-73 22.02382402254281 26.930034289667717 27.785277480612542 23.26086420717693 74-75 21.887715722834102 27.135663284479516 27.702049547596335 23.274571445090046 76-77 21.776516350307222 26.99103944349049 27.76101578654589 23.471428419656405 78-79 21.670664654050455 26.8925567479217 27.779372090624964 23.657406507402882 80-81 21.586669908907723 27.221745079640257 27.626972217775986 23.56461279367604 82-83 21.871823860380417 26.89139916116488 27.943726511969842 23.293050466484864 84-85 21.81404521896812 26.926267211824634 27.57758873417304 23.682098835034203 86-87 21.397319185998185 27.169627078462057 27.879822339152966 23.553231396386792 88-89 21.522329385745458 26.73337235767092 27.871357316868128 23.872940939715495 90-91 21.59984607996845 26.65352005340366 27.90714146736904 23.839492399258848 92-93 21.59618458378973 26.783784119748717 27.78900877289598 23.831022523565576 94-95 21.120867269148892 26.548634514464243 28.01887778278987 24.311620433596996 96-97 21.23003101959998 26.52240475067793 27.954718971818593 24.292845257903494 98-99 21.009474465371934 26.65459845329129 27.843929626517056 24.491997454819725 100 21.04718651356222 26.42707014408407 27.846528534174247 24.679214808179463 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2619.0 1 1849.0 2 2278.5 3 3137.0 4 2075.0 5 1512.0 6 2334.5 7 4389.0 8 6756.5 9 7972.0 10 7141.0 11 5701.0 12 5581.0 13 6192.0 14 6915.5 15 7662.5 16 8393.5 17 9081.5 18 9842.0 19 10695.5 20 11680.5 21 12907.5 22 14521.5 23 16785.0 24 19502.0 25 22865.5 26 28487.0 27 35508.0 28 42340.0 29 52009.0 30 66218.5 31 80357.5 32 99129.0 33 122109.5 34 143898.0 35 163845.0 36 186762.0 37 212717.5 38 224425.0 39 224996.0 40 220878.0 41 216080.5 42 217567.0 43 223306.5 44 232772.5 45 248974.0 46 260548.5 47 264603.0 48 270251.5 49 272179.5 50 266888.0 51 260254.0 52 253062.0 53 243759.0 54 233968.5 55 216806.0 56 196386.0 57 172673.0 58 149049.5 59 131398.5 60 109963.0 61 87511.5 62 69475.5 63 54301.0 64 40519.0 65 28960.5 66 21166.5 67 16181.5 68 12367.0 69 9220.0 70 7134.0 71 5388.0 72 4052.0 73 3078.0 74 2253.0 75 1611.5 76 1113.5 77 687.0 78 403.5 79 227.5 80 136.0 81 81.5 82 51.0 83 39.0 84 25.0 85 15.0 86 9.5 87 8.5 88 7.5 89 2.0 90 1.5 91 1.0 92 0.5 93 0.5 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.029164879992588825 2 0.0018354508221302185 3 7.226184339095348E-5 4 0.021461767487113183 5 5.058329037366744E-4 6 2.8904737356381394E-5 7 2.8904737356381394E-5 8 0.004118925073284349 9 0.010304538867549966 10-11 0.0010333443604906348 12-13 7.948802773004883E-5 14-15 1.4452368678190695E-4 16-17 2.890473735638139E-4 18-19 0.0 20-21 1.517498711210023E-4 22-23 2.529164518683372E-4 24-25 2.8904737356381394E-5 26-27 7.226184339095348E-5 28-29 0.0010333443604906348 30-31 0.002579747809057039 32-33 0.009964908203612484 34-35 0.01921442415765453 36-37 0.022278326317430958 38-39 0.021526803146165042 40-41 0.004827091138515693 42-43 0.005000519562653981 44-45 0.009271194507059332 46-47 0.011735323366690845 48-49 1.1986071963257454 50-51 0.01792093716095646 52-53 0.018701365069578764 54-55 0.009668634645709577 56-57 0.007782600533205689 58-59 0.008447409492402462 60-61 0.005340150226591462 62-63 0.007818731454901166 64-65 0.019395078766131914 66-67 0.029070939596180588 68-69 0.028875832619025012 70-71 0.02611543020149059 72-73 0.023116563700766018 74-75 0.009842063069847863 76-77 0.014690832761380843 78-79 0.008548576073149796 80-81 0.0028687951826208533 82-83 0.014401785387817029 84-85 0.023889765425049222 86-87 0.023210504097174258 88-89 0.03222878215236525 90-91 0.020276673255501546 92-93 0.013397345764682775 94-95 0.006648089591967721 96-97 0.0038804609900942024 98-99 0.0159987721267571 100 0.03391970928771356 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 6919281.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.998345164354625 #Duplication Level Percentage of deduplicated Percentage of total 1 74.62766767950427 43.2828122888664 2 15.104155782950969 17.520320810315464 3 4.902584763510262 8.530254099347221 4 2.034333897181905 4.7195199819321125 5 1.0381101815342555 3.010433631362729 6 0.5837069758780917 2.0312423197091505 7 0.3760850369831981 1.5268616850270418 8 0.24463002271090592 1.1350509195800824 9 0.18129345022107127 0.946324809176259 >10 0.8231343185347364 8.278055160021603 >50 0.04808922849360561 1.932859307052192 >100 0.03227850125986765 3.7382809398093677 >500 0.0027443395866741972 1.0656724882125144 >1k 0.0010502991759794714 0.9794444744781934 >5k 3.3880618579094984E-5 0.10618967057196792 >10k+ 1.0164185573728495E-4 1.1966774145378005 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 25797 0.3728277547912854 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 21742 0.31422339980122216 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13360 0.19308364554062768 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.4452368678190697E-5 0.0 5 0.0 0.0 0.0 1.4452368678190697E-5 0.0 6 0.0 0.0 0.0 1.4452368678190697E-5 0.0 7 0.0 0.0 0.0 1.4452368678190697E-5 0.0 8 0.0 0.0 0.0 1.4452368678190697E-5 0.0 9 0.0 0.0 0.0 2.8904737356381394E-5 0.0 10-11 0.0 0.0 0.0 2.8904737356381394E-5 0.0 12-13 0.0 0.0 0.0 2.8904737356381394E-5 0.0 14-15 0.0 0.0 0.0 5.780947471276279E-5 1.4452368678190697E-5 16-17 2.1678553017286046E-5 0.0 0.0 7.226184339095349E-5 3.6130921695476744E-5 18-19 4.335710603457209E-5 0.0 0.0 1.0839276508643023E-4 4.335710603457209E-5 20-21 4.335710603457209E-5 0.0 0.0 1.8788079281647905E-4 4.335710603457209E-5 22-23 5.780947471276279E-5 0.0 0.0 4.046663229893395E-4 5.780947471276278E-5 24-25 5.780947471276279E-5 0.0 0.0 0.0010333443604906348 7.226184339095348E-5 26-27 5.780947471276279E-5 0.0 0.0 0.0023846408319014648 7.226184339095348E-5 28-29 5.780947471276279E-5 0.0 0.0 0.005838756945989041 7.226184339095348E-5 30-31 5.780947471276279E-5 0.0 0.0 0.018267794009233042 7.226184339095348E-5 32-33 6.503565905185813E-5 0.0 0.0 0.04849492309966888 1.0116658074733488E-4 34-35 9.394039640823952E-5 0.0 0.0 0.09462688392045358 1.0116658074733488E-4 36-37 1.1561894942552557E-4 7.226184339095348E-6 0.0 0.16704770336686717 1.0116658074733488E-4 38-39 1.1561894942552557E-4 1.4452368678190697E-5 0.0 0.27124205535228296 1.0116658074733488E-4 40-41 1.1561894942552557E-4 2.1678553017286046E-5 0.0 0.3846281138170281 1.3007131810371627E-4 42-43 1.1561894942552557E-4 2.8904737356381394E-5 0.0 0.4959763882981483 1.3007131810371627E-4 44-45 1.1561894942552557E-4 2.8904737356381394E-5 0.0 0.6296824771244296 1.3007131810371627E-4 46-47 1.1561894942552557E-4 2.8904737356381394E-5 0.0 0.7896051627329487 1.3729750244281162E-4 48-49 1.1561894942552557E-4 2.8904737356381394E-5 0.0 0.9598685181307134 1.4452368678190695E-4 50-51 1.1561894942552557E-4 2.8904737356381394E-5 0.0 1.1427776961218947 1.4452368678190695E-4 52-53 1.1561894942552557E-4 2.8904737356381394E-5 0.0 1.3202455573057374 1.517498711210023E-4 54-55 1.1561894942552557E-4 2.8904737356381394E-5 0.0 1.5201796255998274 1.5897605546009766E-4 56-57 1.1561894942552557E-4 2.8904737356381394E-5 0.0 1.750535929961509 1.5897605546009766E-4 58-59 1.1561894942552557E-4 2.8904737356381394E-5 0.0 2.0087347225817247 1.5897605546009766E-4 60-61 1.1561894942552557E-4 4.335710603457209E-5 0.0 2.2702575599979244 1.5897605546009766E-4 62-63 1.1561894942552557E-4 4.335710603457209E-5 0.0 2.534555252200337 1.5897605546009766E-4 64-65 1.1561894942552557E-4 4.335710603457209E-5 0.0 2.8011870019442773 1.66202239799193E-4 66-67 1.1561894942552557E-4 4.335710603457209E-5 0.0 3.1018974370313908 1.806546084773837E-4 68-69 1.3007131810371627E-4 4.335710603457209E-5 0.0 3.431042907492845 1.8788079281647905E-4 70-71 1.3007131810371627E-4 4.335710603457209E-5 0.0 3.7908996613954544 2.0233316149466976E-4 72-73 1.3007131810371627E-4 4.335710603457209E-5 0.0 4.140039983923185 2.4569026752924183E-4 74-75 1.3007131810371627E-4 4.335710603457209E-5 0.0 4.480263194976472 2.4569026752924183E-4 76-77 1.3007131810371627E-4 4.335710603457209E-5 0.0 4.852390009886865 2.529164518683372E-4 78-79 1.3007131810371627E-4 4.335710603457209E-5 0.0 5.26484615959375 2.6014263620743254E-4 80-81 1.3007131810371627E-4 4.335710603457209E-5 0.0 5.6949847823784 2.8904737356381396E-4 82-83 1.3007131810371627E-4 4.335710603457209E-5 0.0 6.131352376063351 3.179521109201953E-4 84-85 1.3007131810371627E-4 4.335710603457209E-5 0.0 6.55823198971107 3.179521109201953E-4 86-87 1.3007131810371627E-4 4.335710603457209E-5 0.0 7.007715107971478 3.179521109201953E-4 88 1.3007131810371627E-4 4.335710603457209E-5 0.0 7.359926558843325 3.32404479598386E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 13275 0.0 51.853382 1 GTATCAA 23700 0.0 39.775265 1 TACCTGG 5170 0.0 32.57857 2 ATCAACG 29595 0.0 31.185211 3 CAACGCA 29680 0.0 31.105864 5 TATCAAC 30820 0.0 30.217327 2 TCAACGC 30870 0.0 29.98839 4 AACGCAG 31695 0.0 29.483538 6 GTACATG 29985 0.0 26.195875 1 ACCTGGG 6235 0.0 25.962427 3 ACGCAGA 35725 0.0 25.894989 7 GTACCTG 6770 0.0 25.505901 1 CGCAGAG 36715 0.0 25.196745 8 TACATGG 30875 0.0 24.905779 2 GTGGTAT 6635 0.0 24.327585 1 ACATGGG 30880 0.0 24.265558 3 GCAGAGT 40030 0.0 22.945398 9 TGGTATC 6930 0.0 22.27362 2 GTACTAG 2415 0.0 21.761179 1 CATGGGG 19800 0.0 21.70666 4 >>END_MODULE