FastQCFastQC Report
Mon 6 Jun 2016
ERR1211337_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211337_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7243998
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT253150.34946171989556046No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT203780.2813087469102007No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112250.15495586829261962No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA121400.051.270291
GTATCAA222750.042.6847951
ATCAACG272000.034.173763
CAACGCA273100.034.0702935
TATCAAC279000.033.6562652
TCAACGC282250.032.93254
AACGCAG292050.032.0037546
TACCTGG61100.030.721422
ACGCAGA329850.027.9815887
GTGGTAT52650.027.6111621
CGCAGAG339600.027.134848
GTACATG304000.026.757531
TACATGG312450.025.7017442
GTACCTG78750.025.2705351
ACATGGG320250.024.6375163
GCAGAGT367950.024.4460959
ACCTGGG76550.024.0030783
TGGTATC59050.023.5820432
GTACTAG23150.023.573911
CATGGGG229000.023.4143734
Produced by FastQC (version 0.11.5)