FastQCFastQC Report
Mon 6 Jun 2016
ERR1211337_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211337_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7243998
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT223400.30839323809863006No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190120.26245175661285386No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109410.15103538129082864No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA125350.051.440661
GTATCAA219250.039.5982741
ATCAACG273950.031.187523
CAACGCA275550.031.0696015
TACCTGG60000.030.4956262
TATCAAC283550.030.3951072
TCAACGC282500.030.3932974
AACGCAG293050.029.3901086
GTACATG304250.025.8824561
GTGGTAT56300.025.6735821
ACGCAGA333100.025.6733347
CGCAGAG341600.025.0207758
TACATGG312050.024.7323822
GTACCTG76000.024.5143891
ACCTGGG75150.023.9117853
ACATGGG317400.023.7991333
TGGTATC61850.022.6048032
GCAGAGT377550.022.3017649
CATGGGG226250.022.2513484
TGGGGGG125850.021.9922166
Produced by FastQC (version 0.11.5)