FastQCFastQC Report
Mon 6 Jun 2016
ERR1211336_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211336_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14609500
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT452220.3095383141106814No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT349280.23907731270748486No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT207300.1418939730996954No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA227000.050.723661
GTATCAA420000.042.3430751
CAACGCA501100.034.4424865
ATCAACG505850.034.1285863
TATCAAC534500.032.9373172
TCAACGC532000.032.662564
AACGCAG542050.032.2222336
GTACTAG57650.030.105931
ACGCAGA611150.028.1412327
CGCAGAG629900.027.1750688
GTACATG617100.027.0657541
TACATGG634100.025.9687142
ACATGGG631850.025.1884883
GCAGAGT689950.024.273289
GTGGTAT108050.023.593891
CATGGGG421950.022.1497464
GCATCGT68150.021.649458
GAGTACT369200.021.38263112-13
TAGTACT84950.021.1850934
CTGTGCG81600.020.8461939
Produced by FastQC (version 0.11.5)