FastQCFastQC Report
Mon 6 Jun 2016
ERR1211336_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211336_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14609500
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT396830.27162462781067115No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT320360.21928197405797598No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT197810.13539819980149903No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA226300.051.5117841
GTATCAA413400.041.4571841
CAACGCA514450.032.4834675
ATCAACG517900.032.2817763
TATCAAC543750.031.141492
TCAACGC541800.030.9271454
AACGCAG550650.030.6546046
GTACTAG59300.026.8277681
ACGCAGA626350.026.6426247
GTACATG608100.026.1152921
CGCAGAG644300.025.856688
TACATGG622050.025.0417462
ACATGGG615150.024.4017333
GTGGTAT106050.023.8073521
GCAGAGT712950.023.0957989
CATGGGG399600.021.9586224
GAGTACT381200.020.6782612-13
TCTAACG26950.020.543532
CTGTGCG83800.020.4890589
TGGTATC116850.020.2373492
Produced by FastQC (version 0.11.5)