##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211336_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 14609500 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.12592443273213 33.0 31.0 34.0 30.0 34.0 2 32.26272712960745 34.0 31.0 34.0 30.0 34.0 3 32.328218487970155 34.0 31.0 34.0 30.0 34.0 4 35.96229973647284 37.0 35.0 37.0 35.0 37.0 5 35.867139395598755 37.0 35.0 37.0 35.0 37.0 6 35.88060871350833 37.0 35.0 37.0 35.0 37.0 7 35.79754201033574 37.0 35.0 37.0 33.0 37.0 8 35.781746192545945 37.0 35.0 37.0 33.0 37.0 9 37.39975639138916 39.0 37.0 39.0 34.0 39.0 10-11 37.35148133064102 39.0 37.0 39.0 34.0 39.0 12-13 37.280636880112255 39.0 37.0 39.0 34.0 39.0 14-15 38.52353602108217 40.0 38.0 41.0 34.0 41.0 16-17 38.5114049419898 40.0 38.0 41.0 34.0 41.0 18-19 38.518379787124815 40.0 38.0 41.0 34.0 41.0 20-21 38.48678733016188 40.0 38.0 41.0 34.0 41.0 22-23 38.37776898593381 40.0 38.0 41.0 34.0 41.0 24-25 38.28778476333892 40.0 38.0 41.0 34.0 41.0 26-27 38.19146291796434 40.0 38.0 41.0 33.5 41.0 28-29 38.098390122865254 40.0 37.5 41.0 33.0 41.0 30-31 38.11005527225436 40.0 37.5 41.0 33.0 41.0 32-33 38.05652962113693 40.0 37.0 41.0 33.0 41.0 34-35 37.93532396728156 40.0 37.0 41.0 33.0 41.0 36-37 37.795400971970295 40.0 37.0 41.0 33.0 41.0 38-39 37.68539183408056 40.0 37.0 41.0 33.0 41.0 40-41 37.577369246038536 40.0 37.0 41.0 32.5 41.0 42-43 37.47372066121359 40.0 36.5 41.0 32.0 41.0 44-45 37.31311632841644 40.0 36.0 41.0 31.5 41.0 46-47 37.072884527191206 39.5 36.0 41.0 31.0 41.0 48-49 35.527191313871114 39.0 35.0 41.0 24.5 41.0 50-51 36.25644091173552 39.0 35.0 40.0 29.0 41.0 52-53 36.34975912933365 39.0 35.0 40.0 30.0 41.0 54-55 36.17741230021561 38.5 35.0 40.0 29.0 41.0 56-57 35.96094527533454 38.0 35.0 40.0 29.0 41.0 58-59 35.71763451863514 38.0 34.0 40.0 28.0 41.0 60-61 35.462235155207225 37.5 34.0 40.0 28.0 41.0 62-63 35.141447209007836 37.0 34.0 40.0 28.0 41.0 64-65 34.80754207878435 36.5 34.0 39.5 27.0 41.0 66-67 34.468756254491936 36.0 33.0 39.0 26.5 40.5 68-69 34.11321352544577 35.5 33.0 39.0 26.0 40.0 70-71 33.763150278928094 35.0 33.0 38.0 26.0 40.0 72-73 33.39039375064171 35.0 32.0 37.5 26.0 39.5 74-75 33.03319915123721 35.0 32.0 37.0 25.5 39.0 76-77 32.094491187241175 34.0 30.5 36.0 24.5 38.5 78-79 32.52911458297683 35.0 32.0 36.0 25.0 38.0 80-81 32.40694079195045 35.0 32.0 36.0 25.0 37.0 82-83 32.141933536397545 34.5 31.5 35.0 25.0 37.0 84-85 31.864450118073854 34.0 31.0 35.0 24.5 36.5 86-87 31.611371470618433 34.0 31.0 35.0 24.0 36.0 88-89 31.385690954515898 34.0 31.0 35.0 24.0 36.0 90-91 31.17095434477566 34.0 31.0 35.0 23.0 35.0 92-93 30.984210958622814 34.0 30.5 35.0 23.0 35.0 94-95 30.794359800130053 34.0 30.0 35.0 20.5 35.0 96-97 30.609277593346796 34.0 30.0 35.0 20.0 35.0 98-99 30.397160272425474 34.0 29.0 35.0 20.0 35.0 100 27.926049146103562 31.0 25.0 34.0 15.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.2523466457020582 1101 2 -0.23941745676225068 1101 3 -0.24909100159127462 1101 4 -0.1532868570433834 1101 5 -0.13277182357668948 1101 6 -0.20455548771885645 1101 7 -0.19482377726818356 1101 8 -0.17660251350800138 1101 9 -0.2935117653797761 1101 10-11 -0.28693308556411523 1101 12-13 -0.4093522490044137 1101 14-15 -0.5008660955605464 1101 16-17 -0.47927502042677617 1101 18-19 -0.46049713319219165 1101 20-21 -0.5244632294286333 1101 22-23 -0.5940005837107023 1101 24-25 -0.5615658700364037 1101 26-27 -0.5983708969615193 1101 28-29 -0.6220762013313959 1101 30-31 -0.5887375317610619 1101 32-33 -0.5188506178807657 1101 34-35 -0.5644179527871103 1101 36-37 -0.6123044729331539 1101 38-39 -0.5501045160337839 1101 40-41 -0.5067978064887626 1101 42-43 -0.546865908391915 1101 44-45 -0.5543896640166892 1101 46-47 -0.5377959063855755 1101 48-49 0.6594068120215297 1101 50-51 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1105 68-69 -0.7985449596212462 1105 70-71 -0.8241236865872423 1105 72-73 -0.7797332654230402 1105 74-75 -0.8314702752830883 1105 76-77 -0.8358114356213804 1105 78-79 -0.7261815076488389 1105 80-81 -0.6740230012171402 1105 82-83 -0.6670620946096761 1105 84-85 -0.6607020570720685 1105 86-87 -0.6742147762181432 1105 88-89 -0.7902046498243926 1105 90-91 -0.8195962034631599 1105 92-93 -0.738068057833356 1105 94-95 -0.7662989483573242 1105 96-97 -0.7865986205166138 1105 98-99 -0.8070148039243428 1105 100 -0.8294871262019043 1106 1 -0.25703017229215064 1106 2 -0.2487792683146317 1106 3 -0.29030323417630655 1106 4 -0.16587152089492463 1106 5 -0.23844786340443136 1106 6 -0.3057616963961678 1106 7 -0.30731064071378 1106 8 -0.33319439345129354 1106 9 -0.43028944429095617 1106 10-11 -0.37487279481939595 1106 12-13 -0.3884334519941106 1106 14-15 -0.5351049700183168 1106 16-17 -0.5157652400998742 1106 18-19 -0.5369826908998547 1106 20-21 -0.5566967572938992 1106 22-23 -0.6114618940004846 1106 24-25 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2208 48-49 2.21406247346151 2208 50-51 1.377247446165761 2208 52-53 1.10299784887021 2208 54-55 1.0678836726686427 2208 56-57 1.0700822273212012 2208 58-59 1.0719594119942997 2208 60-61 1.0774430115746583 2208 62-63 1.0944600142325172 2208 64-65 1.129251397055036 2208 66-67 1.1011222798843647 2208 68-69 1.1051699763232605 2208 70-71 1.1415150512676817 2208 72-73 1.1946643018536207 2208 74-75 1.208757661792987 2208 76-77 1.1613473882678989 2208 78-79 1.031190105027271 2208 80-81 0.96442886266221 2208 82-83 0.9752325580400836 2208 84-85 1.0416488040743879 2208 86-87 1.0884767939133617 2208 88-89 1.1111063474838865 2208 90-91 1.0444142716361888 2208 92-93 1.1001619763357233 2208 94-95 1.1144878127779982 2208 96-97 1.144414676482878 2208 98-99 1.244810186191856 2208 100 1.4763258961528294 2209 1 0.34818813376618607 2209 2 0.40676762540403644 2209 3 0.41431504270880026 2209 4 0.26916451729513824 2209 5 0.3663129492081225 2209 6 0.377908069099675 2209 7 0.4166435577365277 2209 8 0.4641615572491631 2209 9 0.6167540102500837 2209 10-11 0.6137694486919898 2209 12-13 0.6436133892507101 2209 14-15 0.7652788907473109 2209 16-17 0.791157396327911 2209 18-19 0.7570921083514222 2209 20-21 0.7769443231577711 2209 22-23 0.7697877559798059 2209 24-25 0.8120229219804429 2209 26-27 0.7990202576165828 2209 28-29 0.8348883466871371 2209 30-31 0.8558953286021236 2209 32-33 0.8174237807139946 2209 34-35 0.8587431868205329 2209 36-37 0.8848364936712443 2209 38-39 0.9302274367828787 2209 40-41 0.9381391716426108 2209 42-43 0.9687355552318522 2209 44-45 0.985699666917327 2209 46-47 0.9693381008220854 2209 48-49 2.2011443715060324 2209 50-51 1.3140667447479046 2209 52-53 1.0847391557649857 2209 54-55 1.062688633794025 2209 56-57 1.0985848533745255 2209 58-59 1.0790560961863775 2209 60-61 1.0181252166144361 2209 62-63 1.0544000304933405 2209 64-65 1.0724959359426336 2209 66-67 1.0291519960587436 2209 68-69 1.093864434859917 2209 70-71 1.089366234177831 2209 72-73 1.0941897794967375 2209 74-75 1.0959347246534392 2209 76-77 1.017020992618157 2209 78-79 1.0097599503146526 2209 80-81 0.9522567619778997 2209 82-83 0.9482283440830557 2209 84-85 1.0316396104919683 2209 86-87 1.040639689641683 2209 88-89 1.0485609715474347 2209 90-91 1.1017173680473071 2209 92-93 1.0692794594166664 2209 94-95 1.1048880656849818 2209 96-97 1.1381566037285467 2209 98-99 1.222396559352461 2209 100 1.4073798468103753 2210 1 0.5452273718002942 2210 2 0.4889339072116883 2210 3 0.4785824715465168 2210 4 0.33111583491977825 2210 5 0.3719323743851959 2210 6 0.42693144676598394 2210 7 0.44568866042848754 2210 8 0.4372718921699317 2210 9 0.5788288491167037 2210 10-11 0.6114951096108712 2210 12-13 0.6249957768127103 2210 14-15 0.7898758786988935 2210 16-17 0.7635392907721297 2210 18-19 0.7557691843461427 2210 20-21 0.7654602456232382 2210 22-23 0.8137085296105369 2210 24-25 0.8344818211994038 2210 26-27 0.8772440206181571 2210 28-29 0.8453632180130768 2210 30-31 0.8937748140597321 2210 32-33 0.8744000271644481 2210 34-35 0.8972411724014222 2210 36-37 0.9285107340555427 2210 38-39 0.9643209152086101 2210 40-41 0.9761416855907683 2210 42-43 0.9233997277336812 2210 44-45 0.9665398135923908 2210 46-47 1.011723008560331 2210 48-49 2.321025818077672 2210 50-51 1.4331768906279692 2210 52-53 1.124648919973346 2210 54-55 1.0972974331254903 2210 56-57 1.1148250720755541 2210 58-59 1.0868942015150225 2210 60-61 1.0557255831189138 2210 62-63 1.0969557800964864 2210 64-65 1.1319409421359055 2210 66-67 1.138070404848996 2210 68-69 1.1387924748339628 2210 70-71 1.130193298210969 2210 72-73 1.1947219052410318 2210 74-75 1.186926798133122 2210 76-77 1.149389991263 2210 78-79 1.0339765506522127 2210 80-81 1.0237156414135313 2210 82-83 1.083608090570685 2210 84-85 1.1087417576792014 2210 86-87 1.1374642400930135 2210 88-89 1.1536671310867952 2210 90-91 1.2412068592530652 2210 92-93 1.1512381426676193 2210 94-95 1.151247068619874 2210 96-97 1.2491742691419034 2210 98-99 1.3158989448921652 2210 100 1.5410631692079235 2211 1 0.4888757556685803 2211 2 0.47053199576414784 2211 3 0.4433882961049562 2211 4 0.32413228520945836 2211 5 0.3755477730967982 2211 6 0.37465786731263506 2211 7 0.44078218885351816 2211 8 0.42530420070801256 2211 9 0.5359315118928123 2211 10-11 0.5731956072074667 2211 12-13 0.5491932789623846 2211 14-15 0.694642163357976 2211 16-17 0.6545868514211222 2211 18-19 0.6860590026458553 2211 20-21 0.6706131896189547 2211 22-23 0.718412508973671 2211 24-25 0.7163169377046614 2211 26-27 0.7619776020449862 2211 28-29 0.768351969228874 2211 30-31 0.8197094639800824 2211 32-33 0.7774034384498805 2211 34-35 0.8185287916906958 2211 36-37 0.8268436445022544 2211 38-39 0.8565099173371351 2211 40-41 0.8638281433514479 2211 42-43 0.7697270983656139 2211 44-45 0.814232332728011 2211 46-47 0.8710069205435289 2211 48-49 2.140424050411582 2211 50-51 1.2865448301833737 2211 52-53 1.064350784903361 2211 54-55 0.9916948901672598 2211 56-57 0.9871142983339993 2211 58-59 0.9722377968970974 2211 60-61 0.921777725039675 2211 62-63 0.9520097686221547 2211 64-65 1.0086601172250695 2211 66-67 1.0304897392254446 2211 68-69 1.043297274597144 2211 70-71 0.9957009736031992 2211 72-73 0.9998582156962001 2211 74-75 1.0024890258998553 2211 76-77 0.7538422121225921 2211 78-79 0.6491898264729627 2211 80-81 0.738604986080226 2211 82-83 0.8187897203757402 2211 84-85 0.9376438030408316 2211 86-87 0.9308110353172232 2211 88-89 0.9672343912043608 2211 90-91 0.997714097662449 2211 92-93 1.0164438065291428 2211 94-95 0.9899811575106447 2211 96-97 1.0198590635485232 2211 98-99 1.096714693190048 2211 100 1.3072101082135745 2212 1 0.5153598067371377 2212 2 0.49290939661169375 2212 3 0.47413254303400265 2212 4 0.30086818148174643 2212 5 0.3609318893105282 2212 6 0.26004291947177904 2212 7 0.21811491474984734 2212 8 0.3239239004533516 2212 9 0.4870090548860162 2212 10-11 0.49986078479340534 2212 12-13 0.5358266715048075 2212 14-15 0.663321888402514 2212 16-17 0.6901388855171859 2212 18-19 0.707476068691399 2212 20-21 0.6977785204132587 2212 22-23 0.6963881956414042 2212 24-25 0.7427143927426059 2212 26-27 0.7793491915990174 2212 28-29 0.7906504681916573 2212 30-31 0.7653245639331558 2212 32-33 0.789821389343274 2212 34-35 0.7725323445732073 2212 36-37 0.791858890294165 2212 38-39 0.8514453564970594 2212 40-41 0.8878231698555652 2212 42-43 0.9093500017424887 2212 44-45 0.952504586769571 2212 46-47 0.9406681993687513 2212 48-49 2.14847421388162 2212 50-51 1.2967847475638479 2212 52-53 1.0599472249717437 2212 54-55 1.024677775182738 2212 56-57 0.9929234838540637 2212 58-59 1.029771331146712 2212 60-61 1.0330868883837283 2212 62-63 1.0282056328227185 2212 64-65 1.0276667443931515 2212 66-67 1.0321995259934766 2212 68-69 1.0422808463098718 2212 70-71 1.0006049270534874 2212 72-73 1.0461842673742225 2212 74-75 1.0888166256821776 2212 76-77 1.040101538481359 2212 78-79 0.8992367502227623 2212 80-81 0.9228632527126948 2212 82-83 0.969398810041497 2212 84-85 0.9198703682746263 2212 86-87 0.9885323278055544 2212 88-89 1.0646111638723177 2212 90-91 1.072096525464385 2212 92-93 1.0894821829893253 2212 94-95 1.0523253036274056 2212 96-97 1.1215593770312111 2212 98-99 1.1336169339445519 2212 100 1.3106223881209544 2213 1 0.41240127112136094 2213 2 0.40038531481665984 2213 3 0.4245691773441749 2213 4 0.2811867272112778 2213 5 0.306138755996507 2213 6 0.32364594049349193 2213 7 0.40356481612039374 2213 8 0.3709023568118113 2213 9 0.4925424817600259 2213 10-11 0.4933458839607283 2213 12-13 0.5119992065006471 2213 14-15 0.6441662379918043 2213 16-17 0.6595006168258024 2213 18-19 0.6285750803091688 2213 20-21 0.5244169248053296 2213 22-23 0.6338983623183623 2213 24-25 0.6930360946725997 2213 26-27 0.7211758647259714 2213 28-29 0.7675812089462539 2213 30-31 0.7471290392717336 2213 32-33 0.75191088393656 2213 34-35 0.7258027090252028 2213 36-37 0.7779936813658708 2213 38-39 0.7477249543939308 2213 40-41 0.7588181152954903 2213 42-43 0.8174521582523369 2213 44-45 0.8245426948818277 2213 46-47 0.6202895683971548 2213 48-49 1.8810451225570972 2213 50-51 1.2004820327095231 2213 52-53 1.0005897430079003 2213 54-55 0.9642565434787684 2213 56-57 0.840473709775388 2213 58-59 0.8355082075434126 2213 60-61 0.8345351668554315 2213 62-63 0.9105809498563175 2213 64-65 0.9356964837173791 2213 66-67 0.9428111300827027 2213 68-69 0.94417437086517 2213 70-71 0.8439763769078894 2213 72-73 0.8780505909037615 2213 74-75 0.981591565247534 2213 76-77 0.9788667913963849 2213 78-79 0.8501763541449705 2213 80-81 0.8228228252207757 2213 82-83 0.8287213592308191 2213 84-85 0.8350194635889814 2213 86-87 0.7097624080052363 2213 88-89 0.8323007106001832 2213 90-91 0.9082174855060394 2213 92-93 0.9535065574976613 2213 94-95 0.974989911007377 2213 96-97 1.0698589069791637 2213 98-99 1.11675115988675 2213 100 1.2821632315586236 2214 1 0.4838896450011134 2214 2 0.43252495630832044 2214 3 0.42104074101880684 2214 4 0.2836161156037562 2214 5 0.2939444564162841 2214 6 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0.3770323836570384 2311 3 0.40454783943566497 2311 4 0.2530731015887895 2311 5 0.30729498523318455 2311 6 0.33497000220611994 2311 7 0.3739542488398513 2311 8 0.17965457743499513 2311 9 0.45580678882596004 2311 10-11 0.36195940753447076 2311 12-13 0.4636689131579459 2311 14-15 0.4389423640179544 2311 16-17 0.4583321697240663 2311 18-19 0.60164216546017 2311 20-21 0.6315384514116928 2311 22-23 0.6736094083247863 2311 24-25 0.6301815794025885 2311 26-27 0.6558944976826737 2311 28-29 0.7178062937917247 2311 30-31 0.5841260429978803 2311 32-33 0.6253070641411682 2311 34-35 0.7425852229968228 2311 36-37 0.7896051837330518 2311 38-39 0.7812660608589113 2311 40-41 0.7496582647381445 2311 42-43 0.8051249619009795 2311 44-45 0.8612144534648039 2311 46-47 0.8945756680317842 2311 48-49 -5.913080634141046 2311 50-51 -0.9802064636865211 2311 52-53 0.41901461417081265 2311 54-55 0.8444322216413198 2311 56-57 0.9430376445559077 2311 58-59 0.9889350168309647 2311 60-61 0.8080917145460518 2311 62-63 0.7247029670811216 2311 64-65 0.8417565684013866 2311 66-67 0.9980011743732078 2311 68-69 1.0216892845979757 2311 70-71 0.9862385228707211 2311 72-73 0.48909766269007804 2311 74-75 0.809326363691163 2311 76-77 0.8854563658074284 2311 78-79 0.8172340272061334 2311 80-81 0.8480662581567842 2311 82-83 0.8775727145825201 2311 84-85 0.8763519399218005 2311 86-87 0.8944813415684472 2311 88-89 0.952716184073747 2311 90-91 0.9890105614732612 2311 92-93 0.9649948129876513 2311 94-95 1.012180995954651 2311 96-97 1.0725994720575507 2311 98-99 1.061919393815522 2311 100 1.3318248144826157 2312 1 0.0284894076489266 2312 2 0.20336745099995568 2312 3 0.1614601969443612 2312 4 -0.037027725167504855 2312 5 0.17799603003496856 2312 6 0.12773298212391637 2312 7 0.24537162859091666 2312 8 0.31515403427902555 2312 9 0.20906219846481378 2312 10-11 0.2253133879977014 2312 12-13 0.2596160155331262 2312 14-15 0.29595246943014075 2312 16-17 0.4305076975117288 2312 18-19 0.2532219281113086 2312 20-21 0.32524541399133255 2312 22-23 0.5719959531551666 2312 24-25 0.6708270489757666 2312 26-27 0.6354220156915673 2312 28-29 0.7191023552628195 2312 30-31 0.7356573403026729 2312 32-33 0.7538635607119062 2312 34-35 0.3185843180730501 2312 36-37 0.6690565168574736 2312 38-39 0.7905013085428934 2312 40-41 0.5709835610727225 2312 42-43 0.619962121748479 2312 44-45 0.767466672614816 2312 46-47 0.809267151526555 2312 48-49 -4.590033512846908 2312 50-51 -0.7591588666303508 2312 52-53 0.5328517816416607 2312 54-55 0.7842136561663295 2312 56-57 0.9016409769457709 2312 58-59 0.9181208109516348 2312 60-61 0.9417520874338408 2312 62-63 0.7473244427965255 2312 64-65 0.9178250225755207 2312 66-67 0.9002955626533975 2312 68-69 0.8544099069673265 2312 70-71 0.8478849684398 2312 72-73 0.9632194350930448 2312 74-75 0.6842038080265027 2312 76-77 0.8410216521699567 2312 78-79 0.511858464635857 2312 80-81 0.6817667214633971 2312 82-83 0.6782728358322743 2312 84-85 0.7406864361515382 2312 86-87 0.8589025177605158 2312 88-89 0.8539390603134471 2312 90-91 0.6703826410495957 2312 92-93 0.8657073292191484 2312 94-95 1.004602776645413 2312 96-97 1.0378146423257206 2312 98-99 1.0671516434185762 2312 100 0.6263047379715125 2313 1 0.0585754293785854 2313 2 0.18875887587389428 2313 3 0.26394890593188336 2313 4 0.04207542541711007 2313 5 0.07859699486908767 2313 6 0.22648291918598318 2313 7 0.14326783381200414 2313 8 0.21009759596555 2313 9 0.4005064942372272 2313 10-11 0.19451365534489184 2313 12-13 -0.022125230534939533 2313 14-15 0.08375271270033835 2313 16-17 -0.012573657797929627 2313 18-19 0.3442956135926778 2313 20-21 0.3239177258822039 2313 22-23 0.4141935252740865 2313 24-25 0.21754315337047103 2313 26-27 0.3206270476039492 2313 28-29 0.31769240063407267 2313 30-31 0.5074492827422077 2313 32-33 0.39688270675766546 2313 34-35 0.3327118713067989 2313 36-37 -0.3326169931505376 2313 38-39 0.1522652378200675 2313 40-41 0.34177478182402155 2313 42-43 0.43666401408563615 2313 44-45 0.39305834957199437 2313 46-47 0.32521066686867073 2313 48-49 -5.466660289932548 2313 50-51 -1.4728302830586912 2313 52-53 0.0043380512276129934 2313 54-55 0.2293807519620401 2313 56-57 0.21440297225870353 2313 58-59 0.24619341987499865 2313 60-61 0.6080518780689701 2313 62-63 0.6666471706026371 2313 64-65 0.7127881373236775 2313 66-67 0.788563490845938 2313 68-69 0.7905184650682031 2313 70-71 0.7368645355249299 2313 72-73 0.544987113364165 2313 74-75 0.6848004492235376 2313 76-77 0.714652480282524 2313 78-79 0.667122447889426 2313 80-81 -0.2427091117881517 2313 82-83 0.1758478003435684 2313 84-85 0.5660618138395428 2313 86-87 0.6671100517879189 2313 88-89 0.7107133673556341 2313 90-91 0.7480864458018814 2313 92-93 0.7901014899331607 2313 94-95 0.7339155372939032 2313 96-97 0.7012538911842547 2313 98-99 0.8351991503825005 2313 100 0.9830826365333252 2314 1 0.06582463658318005 2314 2 0.19977284360570735 2314 3 0.22886325047938527 2314 4 0.09804651070614767 2314 5 0.20140946209080823 2314 6 0.26788029309732764 2314 7 0.13337384168420385 2314 8 0.23756992134988764 2314 9 0.41963860448775847 2314 10-11 0.31413724532117726 2314 12-13 0.19646530408974172 2314 14-15 0.210466609580358 2314 16-17 0.44877706152667685 2314 18-19 0.4994687391173471 2314 20-21 0.5447534205521691 2314 22-23 0.30674761317747823 2314 24-25 0.47096029388926297 2314 26-27 0.4993955959209728 2314 28-29 0.6148648078490098 2314 30-31 0.49869361405321655 2314 32-33 0.4948447114699803 2314 34-35 0.37504443630938056 2314 36-37 0.4839983651506117 2314 38-39 0.5122456781878668 2314 40-41 0.30258047710727 2314 42-43 0.3008670775773936 2314 44-45 0.5441285690896933 2314 46-47 0.6830270049965748 2314 48-49 -6.526529630003033 2314 50-51 -1.5789174463104843 2314 52-53 -0.7999609823570992 2314 54-55 -0.37471179227355833 2314 56-57 -0.03442659603107501 2314 58-59 0.451780935212895 2314 60-61 0.6540924654203479 2314 62-63 0.5053476209404621 2314 64-65 0.6493113724418933 2314 66-67 0.7436091034015249 2314 68-69 0.7615379518821683 2314 70-71 0.5346521094092438 2314 72-73 0.6388256597521078 2314 74-75 0.7341334084769144 2314 76-77 0.6496313452539226 2314 78-79 0.4821562692407113 2314 80-81 0.4830678937011257 2314 82-83 0.48794031276710115 2314 84-85 0.5206333835289918 2314 86-87 0.5792053326478133 2314 88-89 0.5060632018846043 2314 90-91 0.7295819169385034 2314 92-93 0.7802828932196526 2314 94-95 0.8172184886731344 2314 96-97 0.8598211921093935 2314 98-99 0.9121234014880315 2314 100 -0.479237128687803 2315 1 0.10611619267428551 2315 2 0.207589476547966 2315 3 0.30174163814830735 2315 4 0.14277543289482963 2315 5 0.22881190518190664 2315 6 0.27787166628822746 2315 7 0.326204868652205 2315 8 0.29372697442944684 2315 9 0.44379557454398366 2315 10-11 0.2550635351500148 2315 12-13 0.44340798728873665 2315 14-15 0.2603647044073867 2315 16-17 0.39233551587395965 2315 18-19 0.4618755545578921 2315 20-21 0.4150848851235267 2315 22-23 0.4468809004642793 2315 24-25 0.47947197622005433 2315 26-27 0.5345280931514296 2315 28-29 0.6285834487257773 2315 30-31 0.6225605703173116 2315 32-33 0.6903368731949442 2315 34-35 0.7287448343640435 2315 36-37 0.5973092466620571 2315 38-39 0.7152934939462199 2315 40-41 0.7018662344795601 2315 42-43 0.7585122100441808 2315 44-45 0.7807636740969102 2315 46-47 0.6429387879702304 2315 48-49 -4.7721482928437275 2315 50-51 -0.8561851728398793 2315 52-53 0.3018555212817233 2315 54-55 0.25187158044790436 2315 56-57 0.6088665151906341 2315 58-59 0.7249346098319407 2315 60-61 0.6408333393416541 2315 62-63 0.40877216900995705 2315 64-65 0.547175888595504 2315 66-67 -0.1882064991031811 2315 68-69 0.5847523185513595 2315 70-71 0.5067574555187804 2315 72-73 0.6441361298587154 2315 74-75 0.8218706716197133 2315 76-77 0.7637019695759619 2315 78-79 0.7151978986341092 2315 80-81 0.6104219901543715 2315 82-83 0.5896626835494843 2315 84-85 0.625436715108858 2315 86-87 0.6319026656925963 2315 88-89 0.6637723563020259 2315 90-91 0.7512989833051407 2315 92-93 0.7790603564531544 2315 94-95 0.36283151664517277 2315 96-97 0.5137369291739162 2315 98-99 0.5319072918847638 2315 100 0.6154976365534814 2316 1 0.2007232204698468 2316 2 0.23187931872876533 2316 3 0.17843950707025868 2316 4 -0.06304345207286133 2316 5 0.13904379831757296 2316 6 0.12443267788415113 2316 7 0.21039695929051305 2316 8 0.292375263886008 2316 9 0.3921089840753922 2316 10-11 0.3517292929081819 2316 12-13 0.2585361156305055 2316 14-15 0.41647671923603014 2316 16-17 0.3796298187600158 2316 18-19 0.0803576514422275 2316 20-21 0.41090792063899784 2316 22-23 0.5404033114986078 2316 24-25 0.5620685710615163 2316 26-27 0.5884460668069025 2316 28-29 0.5993172019818047 2316 30-31 0.5696542703557412 2316 32-33 0.507664148793296 2316 34-35 0.48291317649295706 2316 36-37 0.38669451818917366 2316 38-39 0.4878120638207477 2316 40-41 0.15281858636686962 2316 42-43 0.09590397509135329 2316 44-45 0.3387559546771257 2316 46-47 0.34641268763948574 2316 48-49 -5.060881460081866 2316 50-51 -1.0187902955002173 2316 52-53 0.2892012766149179 2316 54-55 0.5183349825812655 2316 56-57 0.44868774401000877 2316 58-59 0.32308908083729904 2316 60-61 0.43255395012157294 2316 62-63 0.3467635814460692 2316 64-65 0.39683585037187896 2316 66-67 0.4534937670397383 2316 68-69 0.1408316110251775 2316 70-71 -0.2030973170807826 2316 72-73 -0.10901184751666193 2316 74-75 0.09828394234700966 2316 76-77 0.171664438104024 2316 78-79 0.23053247296373058 2316 80-81 0.18446926863938984 2316 82-83 0.09437560129196498 2316 84-85 0.2781688146314245 2316 86-87 0.32072526633904985 2316 88-89 0.15589324774614255 2316 90-91 0.2054902904427074 2316 92-93 0.29323970689118894 2316 94-95 0.5094723238482572 2316 96-97 0.5692085276429495 2316 98-99 0.5308895584955309 2316 100 -0.23273572728810166 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 4.0 10 8.0 11 19.0 12 60.0 13 279.0 14 1083.0 15 3098.0 16 7496.0 17 15274.0 18 26438.0 19 40597.0 20 58856.0 21 84854.0 22 105751.0 23 111561.0 24 116367.0 25 129313.0 26 153015.0 27 188211.0 28 233379.0 29 285655.0 30 352810.0 31 438108.0 32 552570.0 33 718491.0 34 999288.0 35 1415725.0 36 2224013.0 37 3297242.0 38 2684829.0 39 365077.0 40 29.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.050488165006925 20.05014650598879 14.123827772359238 23.775537556645045 2 15.264561984349045 20.163672161741324 40.47099349150029 24.10077236240934 3 18.64422627267978 25.488894614239733 29.70611290416201 26.160766208918478 4 12.60234493055415 16.70357035859681 38.24313551621717 32.45094919463187 5 13.366605888604338 37.960666248763296 34.65841738744502 14.014310475187342 6 31.40340364924293 37.30845335116283 17.4431479361095 13.844995063484737 7 28.002397618152408 32.20653220209673 21.788639021776174 18.002431157974684 8 26.246773633683024 35.655965698233686 19.586695176175496 18.51056549190779 9 26.363297633073675 15.881566950531342 20.474277925607563 37.280857490787426 10-11 25.251352832817137 26.345792802195632 27.569417216816856 20.833437148170376 12-13 25.585555196846542 23.760929497228496 28.101091417328444 22.55242388859652 14-15 22.95991319336602 25.285686881790646 25.62329295373741 26.131106971105922 16-17 22.27159932884523 28.291173512590863 26.537660871078828 22.899566287485076 18-19 21.997375337515887 27.51031306860486 28.661711328538164 21.83060026534109 20-21 22.948670271584483 26.59633086363676 28.575777506766663 21.879221358012092 22-23 22.934863419180314 26.415911850561795 28.290864342828193 22.358360387429695 24-25 22.577324629541426 26.598466497249447 28.59841933613788 22.225789537071254 26-27 22.479813972934164 26.847082310282357 28.287978215799093 22.385125500984397 28-29 22.22116772260562 26.82598881955849 28.582329612645708 22.370513845190185 30-31 22.678660609459055 26.643223371212787 28.486355676222686 22.191760343105475 32-33 22.58322834060838 26.909335915452704 28.14664822491273 22.360787519026186 34-35 22.702883255342858 26.812833921831846 28.421471073877175 22.062811748948118 36-37 22.682497046146928 26.631749651829416 28.220644643088693 22.46510865893496 38-39 22.61428499982559 26.737803584975072 28.443480059660015 22.204431355539324 40-41 22.55721260354344 26.381108594218677 28.70788749609483 22.353791306143044 42-43 22.415294328157305 26.421171075276607 28.389626718540555 22.77390787802553 44-45 22.42428913931327 26.83315142096837 28.263277306561108 22.47928213315725 46-47 22.50337053838149 26.7112886277869 27.91802505635762 22.86731577747399 48-49 22.853808041421782 26.80847679555483 27.777556667434606 22.56015849558878 50-51 22.6541344529876 26.73445887522943 27.693516552804876 22.9178901189781 52-53 22.47461525464434 26.976832166708064 27.626142583962928 22.92240999468467 54-55 22.446851480692843 26.925828944257773 27.608126411947804 23.019193163101583 56-57 22.698741021383636 26.82036236780242 27.408562045802515 23.072334565011428 58-59 22.363403457930957 26.88507674148402 27.760649043343825 22.990870757241193 60-61 22.523602894525837 27.16248417857286 27.44977093691496 22.864141989986344 62-63 22.526452754623485 26.943728893568995 27.43874193503561 23.09107641677191 64-65 22.219675447835215 26.772486511680384 27.58476719469789 23.423070845786512 66-67 22.432641409289143 26.736237017009756 27.38351734004945 23.44760423365165 68-69 22.19764768438529 26.79825450144539 27.51824680830844 23.48585100586088 70-71 22.284126291147267 26.493017588690854 27.58631608896097 23.636540031200912 72-73 22.446332533566142 26.729025435253174 27.37015028386549 23.454491747315192 74-75 22.362023044100546 26.899845182460986 27.28309663007617 23.455035143362302 76-77 22.23416855999253 26.76974738647096 27.26073650627091 23.7353475472656 78-79 22.155573336907555 26.655144916459555 27.32275109384636 23.866530652786533 80-81 22.079369806253222 27.012218313736007 27.19784583500367 23.710566045007102 82-83 22.400047044497235 26.60546363782802 27.554541187108196 23.439948130566552 84-85 22.439038400525593 26.634753065144878 27.137036442152052 23.789172092177473 86-87 21.882684938135725 26.888649232337176 27.43828994035158 23.790375889175518 88-89 22.122876009965555 26.47239184410833 27.406135392249592 23.99859675367652 90-91 22.201862932098575 26.417067809683648 27.42563999202957 23.955429266188204 92-93 22.2450086646594 26.52428726553152 27.25094255639559 23.979761513413482 94-95 21.787375550596146 26.29986211886528 27.526547295180116 24.38621503535846 96-97 21.879620979763647 26.247125698179186 27.48211246060429 24.391140861452875 98-99 21.64738228021658 26.35910023927447 27.4199511497124 24.573566330796552 100 21.776856032467993 26.141058666134054 27.242474166295267 24.83961113510269 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1963.0 1 1402.5 2 1057.5 3 1316.0 4 1138.0 5 1091.0 6 1843.0 7 3669.0 8 5999.0 9 7487.5 10 7222.0 11 6526.0 12 6993.0 13 7987.0 14 9263.0 15 10758.0 16 12169.0 17 13640.5 18 15033.0 19 16519.5 20 18264.5 21 20425.5 22 23341.5 23 27170.5 24 32051.5 25 38454.5 26 48120.0 27 60082.5 28 73724.0 29 95709.5 30 126123.0 31 156506.5 32 196156.0 33 244054.5 34 289116.5 35 330401.5 36 380632.0 37 436634.0 38 461309.0 39 464414.5 40 456608.0 41 443159.5 42 445604.5 43 466628.5 44 493925.0 45 531475.5 46 562839.5 47 580522.0 48 600979.0 49 605097.5 50 590694.0 51 580066.0 52 567829.5 53 546856.0 54 526944.0 55 489861.0 56 443130.5 57 387111.5 58 332104.5 59 293348.0 60 244289.5 61 191970.0 62 151906.0 63 119270.5 64 87553.5 65 60097.0 66 42808.0 67 32273.5 68 23537.5 69 16089.0 70 11776.0 71 8773.0 72 6429.5 73 4597.0 74 3243.0 75 2168.5 76 1349.0 77 728.0 78 406.0 79 244.5 80 147.0 81 88.5 82 60.5 83 39.5 84 24.0 85 19.0 86 12.5 87 7.0 88 6.0 89 4.5 90 2.5 91 1.5 92 1.0 93 1.5 94 2.0 95 0.5 96 0.5 97 0.5 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.029398678941784457 2 0.0020260789212498717 3 8.213833464526506E-5 4 0.020616721995961533 5 4.243813956672028E-4 6 2.737944488175502E-5 7 6.844861220438755E-6 8 0.003922105479311407 9 0.010150929189910674 10-11 0.001067798350388446 12-13 7.187104281460694E-5 14-15 1.1978507135767822E-4 16-17 3.6277764468325405E-4 18-19 1.711215305109689E-5 20-21 8.898319586570382E-5 22-23 2.4983743454601456E-4 24-25 3.764673671241316E-5 26-27 8.898319586570382E-5 28-29 0.0010164618912351553 30-31 0.002382011704712687 32-33 0.009206338341490126 34-35 0.018443478558472226 36-37 0.021588692289263835 38-39 0.020989766932475444 40-41 0.004490228960607824 42-43 0.005051507580683802 44-45 0.008730620486669633 46-47 0.011434340668742942 48-49 1.1963208870940143 50-51 0.017865087785345152 52-53 0.018453745850302888 54-55 0.009278209384304733 56-57 0.007601218385297238 58-59 0.008001642766692905 60-61 0.005143913207159725 62-63 0.007508812758821314 64-65 0.019083473082583253 66-67 0.028994832129778568 68-69 0.029258359286765463 70-71 0.026260310072213286 72-73 0.02309798418837058 74-75 0.009144734590506177 76-77 0.01401827577945857 78-79 0.008124850268660803 80-81 0.002775591224887915 82-83 0.014052500085560764 84-85 0.023970703993976522 86-87 0.023361511345357473 88-89 0.0328142646907834 90-91 0.020599609842910434 92-93 0.012909408261747494 94-95 0.006177487251445977 96-97 0.003542215681577056 98-99 0.0151716348951025 100 0.03312228344570314 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1.46095E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.373978209628675 #Duplication Level Percentage of deduplicated Percentage of total 1 69.2277051208252 34.180472041382224 2 15.781954724929575 15.584357773880383 3 6.347559864958133 9.40212847370269 4 3.1163530702689766 6.154669943398795 5 1.6855225353826817 4.16104764669113 6 1.0025919583407183 2.970117210255814 7 0.6240540185466875 2.1568420659348746 8 0.4533966175943436 1.790879577393797 9 0.3291530622230923 1.4626436509651957 >10 1.3156082621454221 10.903237789673295 >50 0.06632625074646119 2.239260997062388 >100 0.04221340144052191 4.18488730535273 >500 0.005200291699240684 1.7653570359507285 >1k 0.0022654342151262317 1.8738167769854281 >5k 2.3846670946634116E-5 0.09295612567302797 >10k+ 7.154001283990234E-5 1.0773255856975374 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 39683 0.27162462781067115 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 32036 0.21928197405797598 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19781 0.13539819980149903 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.737944488175502E-5 2 0.0 0.0 0.0 0.0 2.737944488175502E-5 3 0.0 0.0 0.0 0.0 2.737944488175502E-5 4 0.0 0.0 0.0 0.0 4.791402854307129E-5 5 0.0 0.0 0.0 0.0 4.791402854307129E-5 6 0.0 0.0 0.0 0.0 7.529347342482632E-5 7 0.0 0.0 0.0 0.0 7.529347342482632E-5 8 0.0 0.0 0.0 0.0 7.529347342482632E-5 9 0.0 0.0 0.0 6.844861220438755E-6 1.0951777952702008E-4 10-11 0.0 0.0 0.0 6.844861220438755E-6 1.0951777952702008E-4 12-13 0.0 0.0 0.0 1.368972244087751E-5 1.1294021013723946E-4 14-15 0.0 6.844861220438755E-6 0.0 2.3957014271535642E-5 1.3005236318833636E-4 16-17 0.0 1.368972244087751E-5 0.0 4.791402854307129E-5 1.5743180807009137E-4 18-19 0.0 1.368972244087751E-5 0.0 5.475888976351004E-5 1.5743180807009137E-4 20-21 0.0 1.368972244087751E-5 0.0 1.1636264074745884E-4 1.5743180807009137E-4 22-23 0.0 1.711215305109689E-5 0.0 3.045963243095246E-4 1.985009753927239E-4 24-25 0.0 5.133645915329067E-5 0.0 8.282282076730894E-4 2.5325986515623396E-4 26-27 0.0 6.844861220438756E-5 0.0 0.001899448988671755 2.601047263766727E-4 28-29 0.0 6.844861220438756E-5 0.0 0.00555460488038605 2.601047263766727E-4 30-31 0.0 6.844861220438756E-5 0.0 0.01848454772579486 2.80639310037989E-4 32-33 0.0 6.844861220438756E-5 0.0 0.04875936890379548 3.35398199801499E-4 34-35 0.0 6.844861220438756E-5 0.0 0.09532495978644033 3.730449365139122E-4 36-37 0.0 8.556076525548446E-5 0.0 0.1686163113042883 3.7646736712413156E-4 38-39 0.0 1.0951777952702008E-4 0.0 0.2708237790478798 3.7646736712413156E-4 40-41 0.0 1.1978507135767822E-4 0.0 0.3794311920325816 3.833122283445703E-4 42-43 0.0 1.3005236318833636E-4 0.0 0.4878435264725008 3.8673465895478973E-4 44-45 3.4224306102193776E-6 1.3005236318833636E-4 0.0 0.6194325610048257 4.038468120058866E-4 46-47 1.368972244087751E-5 1.3005236318833636E-4 0.0 0.7749101611964817 4.34648687497861E-4 48-49 1.368972244087751E-5 1.403196550189945E-4 0.0 0.9437078613231117 4.791402854307129E-4 50-51 1.368972244087751E-5 1.4374208562921388E-4 0.0 1.1232211916903383 4.791402854307129E-4 52-53 1.368972244087751E-5 1.4374208562921388E-4 0.0 1.3002737944488176 5.202094527533454E-4 54-55 1.368972244087751E-5 1.5743180807009137E-4 0.0 1.49484924193162 5.647010506861973E-4 56-57 1.368972244087751E-5 1.5743180807009137E-4 0.0 1.7178069064649715 5.71545911906636E-4 58-59 1.368972244087751E-5 1.5743180807009137E-4 0.0 1.9679523597659059 5.783907731270749E-4 60-61 1.368972244087751E-5 1.7454396112118826E-4 0.0 2.2272049009206336 5.955029261781717E-4 62-63 1.368972244087751E-5 1.848112529518464E-4 0.0 2.489503405318457 6.297272322803655E-4 64-65 1.368972244087751E-5 1.848112529518464E-4 0.0 2.756381121872754 6.297272322803655E-4 66-67 1.368972244087751E-5 2.0534583661316268E-4 0.0 3.0534412539785754 6.331496628905848E-4 68-69 1.711215305109689E-5 2.0534583661316268E-4 0.0 3.3789486293165405 6.399945241110236E-4 70-71 2.0534583661316266E-5 2.0876826722338206E-4 0.0 3.7322906328074197 6.947534138745337E-4 72-73 2.0534583661316266E-5 2.1219069783360144E-4 0.0 4.083911153701359 7.734693179095793E-4 74-75 2.0534583661316266E-5 2.1219069783360144E-4 0.0 4.431020911051029 7.734693179095793E-4 76-77 2.0534583661316266E-5 2.2245798966425955E-4 0.0 4.807799719360689 7.871590403504569E-4 78-79 2.0534583661316266E-5 2.2588042027447893E-4 0.0 5.2203942640062975 8.008487627913344E-4 80-81 2.0534583661316266E-5 2.293028508846983E-4 0.0 5.654491940175912 8.213833464526506E-4 82-83 2.0534583661316266E-5 2.3614771210513707E-4 0.0 6.096067627228858 8.829870974365995E-4 84-85 2.0534583661316266E-5 2.3957014271535644E-4 0.0 6.523854341353229 8.898319586570382E-4 86-87 2.0534583661316266E-5 2.464150039357952E-4 0.0 6.9660186864711315 8.898319586570382E-4 88 2.0534583661316266E-5 2.464150039357952E-4 0.0 7.315267462952189 9.172114035387932E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 22630 0.0 51.511784 1 GTATCAA 41340 0.0 41.457184 1 CAACGCA 51445 0.0 32.483467 5 ATCAACG 51790 0.0 32.281776 3 TATCAAC 54375 0.0 31.14149 2 TCAACGC 54180 0.0 30.927145 4 AACGCAG 55065 0.0 30.654604 6 GTACTAG 5930 0.0 26.827768 1 ACGCAGA 62635 0.0 26.642624 7 GTACATG 60810 0.0 26.115292 1 CGCAGAG 64430 0.0 25.85668 8 TACATGG 62205 0.0 25.041746 2 ACATGGG 61515 0.0 24.401733 3 GTGGTAT 10605 0.0 23.807352 1 GCAGAGT 71295 0.0 23.095798 9 CATGGGG 39960 0.0 21.958622 4 GAGTACT 38120 0.0 20.67826 12-13 TCTAACG 2695 0.0 20.54353 2 CTGTGCG 8380 0.0 20.489058 9 TGGTATC 11685 0.0 20.237349 2 >>END_MODULE