FastQCFastQC Report
Mon 6 Jun 2016
ERR1211335_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211335_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9291608
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT319660.34403087172855334No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT252200.27142772273647364No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148950.1603059448913471No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA148950.052.513281
GTATCAA271450.041.949071
CAACGCA338200.032.9702845
ATCAACG338050.032.969953
TATCAAC347050.032.430352
TCAACGC349900.031.9613134
AACGCAG361850.031.126726
TACCTGG74500.030.5555522
GTACATG372100.028.4406971
ACGCAGA404050.027.469337
TACATGG385100.027.0154742
CGCAGAG415850.026.553138
ACATGGG393400.025.8587843
GTGGTAT71700.025.4524731
GTACCTG96400.024.2491651
CATGGGG274750.023.926084
GCAGAGT452550.023.8508959
ACCTGGG94400.023.5436443
ATGGGGG184800.022.0452845
TGGTATC78500.021.9286792
Produced by FastQC (version 0.11.5)