##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211335_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9291608 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.321373544815923 31.0 31.0 34.0 28.0 34.0 2 31.334838921314805 31.0 31.0 34.0 28.0 34.0 3 31.381582498960352 31.0 31.0 34.0 28.0 34.0 4 34.99063154622967 37.0 35.0 37.0 32.0 37.0 5 35.03607362686846 37.0 35.0 37.0 32.0 37.0 6 35.09941293261618 37.0 35.0 37.0 32.0 37.0 7 35.029942395331354 37.0 35.0 37.0 32.0 37.0 8 35.02861732866905 37.0 35.0 37.0 32.0 37.0 9 36.57302137584797 39.0 37.0 39.0 32.0 39.0 10-11 36.50138662758911 39.0 37.0 39.0 32.0 39.0 12-13 36.415262621927226 39.0 37.0 39.0 32.0 39.0 14-15 37.53755211153979 40.0 37.0 41.0 32.0 41.0 16-17 37.529734196707395 40.0 37.0 41.0 32.0 41.0 18-19 37.528197379829194 40.0 37.0 41.0 32.0 41.0 20-21 37.49353050623746 40.0 37.0 41.0 31.0 41.0 22-23 37.37159176323409 40.0 37.0 41.0 31.0 41.0 24-25 37.26187684628968 40.0 37.0 41.0 31.0 41.0 26-27 37.115837484749676 40.0 36.0 41.0 30.5 41.0 28-29 36.9713641061913 39.5 36.0 41.0 30.0 41.0 30-31 36.78659167498242 39.0 36.0 41.0 30.0 41.0 32-33 36.65849732360642 39.0 35.5 41.0 30.0 41.0 34-35 36.51586689838831 39.0 35.0 41.0 30.0 41.0 36-37 36.42102637132346 39.0 35.0 41.0 29.0 41.0 38-39 36.269158094056486 39.0 35.0 40.5 29.0 41.0 40-41 36.10398302425156 39.0 35.0 40.0 28.0 41.0 42-43 35.95292488662888 39.0 35.0 40.0 27.0 41.0 44-45 35.818210045021274 38.5 35.0 40.0 27.0 41.0 46-47 35.65313398929443 38.0 34.5 40.0 26.5 41.0 48-49 35.41243361751809 38.0 34.0 40.0 26.0 41.0 50-51 34.73674960243695 37.5 33.5 39.5 24.5 40.5 52-53 34.98150938997857 38.0 33.5 40.0 25.5 40.5 54-55 35.341117812977046 38.0 34.0 40.0 26.0 41.0 56-57 35.242240417374475 38.0 34.0 40.0 26.0 41.0 58-59 35.02011465614994 38.0 34.0 40.0 26.0 41.0 60-61 34.74631872115138 37.5 33.5 40.0 25.0 41.0 62-63 34.46901375951289 37.0 33.0 40.0 25.0 41.0 64-65 34.18964769069035 36.5 33.0 40.0 24.5 41.0 66-67 33.96108671394661 36.0 33.0 39.0 24.0 41.0 68-69 33.61761758567516 35.5 32.0 39.0 23.0 41.0 70-71 33.270568883233125 35.0 32.0 38.5 23.0 40.0 72-73 32.90567918922107 35.0 32.0 37.5 22.5 40.0 74-75 32.53749264928094 35.0 31.0 37.0 22.0 39.0 76-77 32.18619791106125 35.0 31.0 36.5 21.5 39.0 78-79 31.825355148430713 35.0 31.0 36.0 20.0 39.0 80-81 31.50207671266373 34.5 30.5 36.0 20.0 37.5 82-83 31.19740334503995 34.0 30.0 35.0 20.0 37.0 84-85 30.900645991522673 34.0 30.0 35.0 19.0 36.5 86-87 30.65113611120917 34.0 30.0 35.0 18.5 36.0 88-89 30.397387352113867 34.0 29.0 35.0 18.0 36.0 90-91 30.143166930847705 34.0 29.0 35.0 18.0 35.5 92-93 29.913287775377523 34.0 29.0 35.0 17.0 35.0 94-95 29.692341088862122 34.0 29.0 35.0 15.5 35.0 96-97 29.489718195171385 34.0 28.5 35.0 15.0 35.0 98-99 29.253442084513253 34.0 27.0 35.0 12.5 35.0 100 27.54444343756215 32.0 24.0 34.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.3715474200531048 1101 2 -0.3589667165078261 1101 3 -0.38726688541715504 1101 4 -0.24422349341040928 1101 5 -0.33430890600787677 1101 6 -0.3171615748335981 1101 7 -0.36224247824005573 1101 8 -0.4694495545587074 1101 9 -0.5117334638251307 1101 10-11 -0.5130141833129898 1101 12-13 -0.4546934820676256 1101 14-15 -0.5074128645660636 1101 16-17 -0.5353135602915771 1101 18-19 -0.6442720727068547 1101 20-21 -0.6434745849671728 1101 22-23 -0.6555060586063348 1101 24-25 -0.5939305976176783 1101 26-27 -0.7084942892087795 1101 28-29 -0.7241439066161277 1101 30-31 -0.7322742181912147 1101 32-33 -0.7819241543727884 1101 34-35 -0.7127853608815116 1101 36-37 -0.8195585588091774 1101 38-39 -0.8747410178288604 1101 40-41 -0.8516525624919424 1101 42-43 -0.8479183159504302 1101 44-45 -0.937407268056738 1101 46-47 -0.8958220603123408 1101 48-49 -0.8883737472178126 1101 50-51 -0.8073271247593752 1101 52-53 -0.6884065366229919 1101 54-55 -0.624850022714341 1101 56-57 -0.5838745367669489 1101 58-59 -0.7081055338807616 1101 60-61 -0.6908788027783075 1101 62-63 -0.6626029552508967 1101 64-65 -0.5839472841355473 1101 66-67 -0.6706410120726858 1101 68-69 -0.7197823668407324 1101 70-71 -0.7167902789283502 1101 72-73 -0.8137487007131199 1101 74-75 -0.8910360820973651 1101 76-77 -0.7828446174404391 1101 78-79 -0.8480257589513371 1101 80-81 -0.9353446763956583 1101 82-83 -0.9628721628401493 1101 84-85 -0.9181645815084778 1101 86-87 -0.8757098216595374 1101 88-89 -0.9171028654714277 1101 90-91 -0.9871834254856182 1101 92-93 -1.09124910343046 1101 94-95 -1.1788659486018922 1101 96-97 -1.22845462355874 1101 98-99 -1.180873608146726 1101 100 -1.2452672926102437 1102 1 -0.4328258875706794 1102 2 -0.4174175849970361 1102 3 -0.3724243355449772 1102 4 -0.3071246791767024 1102 5 -0.301505182371109 1102 6 -0.30348658660301453 1102 7 -0.2958193239814193 1102 8 -0.3442174774270157 1102 9 -0.45027307054180454 1102 10-11 -0.3806623835683709 1102 12-13 -0.4660112453895735 1102 14-15 -0.5953677725802251 1102 16-17 -0.5966502207045679 1102 18-19 -0.6080385914576922 1102 20-21 -0.6466060712809494 1102 22-23 -0.6678130608924278 1102 24-25 -0.7337348319670625 1102 26-27 -0.6659101132157517 1102 28-29 -0.6986668315573112 1102 30-31 -0.6696278452450883 1102 32-33 -0.6315042355666804 1102 34-35 -0.6624283169727079 1102 36-37 -0.7736192433508648 1102 38-39 -0.8553225458136637 1102 40-41 -0.9622125621247974 1102 42-43 -0.87528834088058 1102 44-45 -0.865337450430971 1102 46-47 -0.9380347760175667 1102 48-49 -0.8557973200788211 1102 50-51 -0.8134180146948111 1102 52-53 -0.6838737508946622 1102 54-55 -0.5289924148316345 1102 56-57 -0.6682821137677664 1102 58-59 -0.6504793499039394 1102 60-61 -0.7000094713721978 1102 62-63 -0.6468845529866627 1102 64-65 -0.6913498713220321 1102 66-67 -0.670946992772528 1102 68-69 -0.7469855426148442 1102 70-71 -0.6981286366173549 1102 72-73 -0.7072171148333268 1102 74-75 -0.7931406761678019 1102 76-77 -0.748773391751925 1102 78-79 -0.813115355881294 1102 80-81 -0.8268183483677625 1102 82-83 -0.8140752440885421 1102 84-85 -0.9213585079334976 1102 86-87 -0.9195496437321928 1102 88-89 -0.9668889104648599 1102 90-91 -0.9722143246852184 1102 92-93 -0.9742813031235187 1102 94-95 -1.0555337658413393 1102 96-97 -1.068078788034768 1102 98-99 -1.0236823994567388 1102 100 -1.0528734079891002 1103 1 -0.41677602517365386 1103 2 -0.4100666815059384 1103 3 -0.46017078669213163 1103 4 -0.29209464663849616 1103 5 -0.31518118932871886 1103 6 -0.374652351487498 1103 7 -0.4069937740546905 1103 8 -0.39732670970336414 1103 9 -0.522055979608048 1103 10-11 -0.5936798211403698 1103 12-13 -0.6279263946958693 1103 14-15 -0.6628529903763436 1103 16-17 -0.709201228057843 1103 18-19 -0.6754889231845382 1103 20-21 -0.779363405158783 1103 22-23 -0.7722312600251797 1103 24-25 -0.7143566622614728 1103 26-27 -0.686076571854862 1103 28-29 -0.6855030634862587 1103 30-31 -0.7633879829237884 1103 32-33 -0.8068576803984016 1103 34-35 -0.9087259493959579 1103 36-37 -0.9855577771419917 1103 38-39 -1.011811148346517 1103 40-41 -0.9576492251905293 1103 42-43 -0.9142533556157417 1103 44-45 -1.0369713417557662 1103 46-47 -1.104613653199813 1103 48-49 -1.0710729027299308 1103 50-51 -1.0155617970416344 1103 52-53 -0.8809755746627701 1103 54-55 -0.8917161796828097 1103 56-57 -0.8295950576627789 1103 58-59 -0.8479999801322649 1103 60-61 -0.8167934464536089 1103 62-63 -0.8968000791479867 1103 64-65 -0.8086373504166033 1103 66-67 -0.7940505619392937 1103 68-69 -0.7811064156058762 1103 70-71 -0.780831786900194 1103 72-73 -0.9242834066323518 1103 74-75 -0.8762857188874094 1103 76-77 -0.8655863628733016 1103 78-79 -0.8310425749661974 1103 80-81 -0.7925088542001397 1103 82-83 -0.93481056068957 1103 84-85 -0.8981846083442306 1103 86-87 -1.0506814697602813 1103 88-89 -1.0784176705096513 1103 90-91 -1.0771236734612764 1103 92-93 -1.019188569420276 1103 94-95 -1.191454332612281 1103 96-97 -1.2810379664053322 1103 98-99 -1.2563831529918268 1103 100 -1.2863747914715056 1104 1 -0.35325443707312587 1104 2 -0.4102309435344935 1104 3 -0.4409445113259203 1104 4 -0.24926148079189403 1104 5 -0.22711966077689993 1104 6 -0.3001431717798795 1104 7 -0.27866536577048606 1104 8 -0.30787220277268545 1104 9 -0.39779650638897834 1104 10-11 -0.36676630030748925 1104 12-13 -0.4433960028728734 1104 14-15 -0.5521514674908659 1104 16-17 -0.5551862808063461 1104 18-19 -0.5812314422504912 1104 20-21 -0.6047659038026296 1104 22-23 -0.5888079465631222 1104 24-25 -0.6251916798418549 1104 26-27 -0.6649969086327516 1104 28-29 -0.6452370536322221 1104 30-31 -0.7270859164539516 1104 32-33 -0.7420722842641041 1104 34-35 -0.7355541918365347 1104 36-37 -0.7477153946706636 1104 38-39 -0.755946593255949 1104 40-41 -0.7886290279595798 1104 42-43 -0.7629512426205949 1104 44-45 -0.8348460523333969 1104 46-47 -0.7797525088495334 1104 48-49 -0.7008991163242442 1104 50-51 -0.7409215635705095 1104 52-53 -0.704982551192586 1104 54-55 -0.692162277024984 1104 56-57 -0.7209737697730816 1104 58-59 -0.6500683149286175 1104 60-61 -0.5747377159309579 1104 62-63 -0.7346676248276083 1104 64-65 -0.7825536922122609 1104 66-67 -0.7925615806224684 1104 68-69 -0.6836998102926941 1104 70-71 -0.6338564949512318 1104 72-73 -0.6595301841350505 1104 74-75 -0.7050414338234212 1104 76-77 -0.6941924521430103 1104 78-79 -0.7292396505986147 1104 80-81 -0.7559976808367814 1104 82-83 -0.7643556440989094 1104 84-85 -0.7707351198735601 1104 86-87 -0.8805126904270928 1104 88-89 -0.8946848121544981 1104 90-91 -0.843360051726453 1104 92-93 -0.876346240855046 1104 94-95 -0.922260578834404 1104 96-97 -0.9462219737498465 1104 98-99 -0.9800662355897565 1104 100 -0.9839712833206526 1105 1 -0.38945933187656223 1105 2 -0.43434356465894197 1105 3 -0.41172711642534665 1105 4 -0.34896078935965136 1105 5 -0.2898112824177801 1105 6 -0.3218330602632662 1105 7 -0.26840434637779964 1105 8 -0.3381186920388757 1105 9 -0.4888629283756174 1105 10-11 -0.5748034214089515 1105 12-13 -0.5282643091134744 1105 14-15 -0.6460174281275499 1105 16-17 -0.6966926237096942 1105 18-19 -0.6225649040439123 1105 20-21 -0.6458717265084815 1105 22-23 -0.6610087799907305 1105 24-25 -0.6576017644766736 1105 26-27 -0.6139059505940239 1105 28-29 -0.7224798093460478 1105 30-31 -0.7403939028473729 1105 32-33 -0.7300984498187688 1105 34-35 -0.8101373101002096 1105 36-37 -0.769041694133783 1105 38-39 -0.8106204089064164 1105 40-41 -0.8576459039534043 1105 42-43 -0.8048712305139176 1105 44-45 -0.767507911712805 1105 46-47 -0.8414758592975602 1105 48-49 -0.8294479765746345 1105 50-51 -0.7903390480067927 1105 52-53 -0.6899081376363654 1105 54-55 -0.7311585050136316 1105 56-57 -0.7784307131439192 1105 58-59 -0.6777172345665647 1105 60-61 -0.717056860834596 1105 62-63 -0.7138706502865446 1105 64-65 -0.710183138688464 1105 66-67 -0.7427186369073482 1105 68-69 -0.681758302481235 1105 70-71 -0.5803872099290714 1105 72-73 -0.5943078022084691 1105 74-75 -0.6506255239671042 1105 76-77 -0.6726043878148005 1105 78-79 -0.6894145718794924 1105 80-81 -0.7209897236432319 1105 82-83 -0.7200776650772411 1105 84-85 -0.7135446631320974 1105 86-87 -0.7504178903078689 1105 88-89 -0.7374711483038112 1105 90-91 -0.7839622327469087 1105 92-93 -0.794546436021303 1105 94-95 -0.7679198945197108 1105 96-97 -0.8737828130569447 1105 98-99 -0.8818397025935809 1105 100 -1.007357832759368 1106 1 -0.45274136535493525 1106 2 -0.5371491280927252 1106 3 -0.5023205761969862 1106 4 -0.4489170155313147 1106 5 -0.34561833225506433 1106 6 -0.36691480715944635 1106 7 -0.4082939558267995 1106 8 -0.3734934005112649 1106 9 -0.5175367455620261 1106 10-11 -0.5809596423372909 1106 12-13 -0.548278409158776 1106 14-15 -0.675247951488295 1106 16-17 -0.6757547515626428 1106 18-19 -0.7018390555850402 1106 20-21 -0.7663780901320862 1106 22-23 -0.6805782102345006 1106 24-25 -0.7164540546847107 1106 26-27 -0.658916224070552 1106 28-29 -0.7436750480352288 1106 30-31 -0.7730844586902563 1106 32-33 -0.7881345620354807 1106 34-35 -0.838002582571761 1106 36-37 -0.8294906102349984 1106 38-39 -0.9162199369011361 1106 40-41 -0.9826831302445029 1106 42-43 -0.9495411644612517 1106 44-45 -0.9343245891441754 1106 46-47 -0.9527509170337325 1106 48-49 -0.9907542956564512 1106 50-51 -0.9755562779470353 1106 52-53 -0.8255941950762065 1106 54-55 -0.8746010504736645 1106 56-57 -0.8605880121318776 1106 58-59 -0.7922553955202503 1106 60-61 -0.8324354567830952 1106 62-63 -0.8596999485097214 1106 64-65 -0.8590640063477082 1106 66-67 -0.926656243710319 1106 68-69 -0.9391954441376384 1106 70-71 -0.9748057952426237 1106 72-73 -0.8911088569114511 1106 74-75 -0.869957071391795 1106 76-77 -0.8939286507236446 1106 78-79 -0.8108057269180264 1106 80-81 -0.9367516451262787 1106 82-83 -0.8675223843990807 1106 84-85 -0.8172849355043539 1106 86-87 -0.8547630849451657 1106 88-89 -0.9138277662419725 1106 90-91 -0.8097062355602951 1106 92-93 -0.8985535751540219 1106 94-95 -0.9342975691787387 1106 96-97 -1.01464390093059 1106 98-99 -0.9976851531740394 1106 100 -0.9950612135659895 1107 1 -0.5841663119056193 1107 2 -0.5111651863824385 1107 3 -0.5100185236199657 1107 4 -0.43548069146612534 1107 5 -0.3665913115906534 1107 6 -0.4354759267697048 1107 7 -0.4124214703226272 1107 8 -0.43258987122189296 1107 9 -0.5652986249304632 1107 10-11 -0.5281329070225667 1107 12-13 -0.54129323368592 1107 14-15 -0.6417136512154329 1107 16-17 -0.7112523916692126 1107 18-19 -0.6664857145125822 1107 20-21 -0.7048416886704914 1107 22-23 -0.7143345349255412 1107 24-25 -0.7457969543666181 1107 26-27 -0.758911504335515 1107 28-29 -0.7977725046575799 1107 30-31 -0.7768783860957527 1107 32-33 -0.8560051419272057 1107 34-35 -0.8318667327440963 1107 36-37 -0.8247812757129793 1107 38-39 -0.7603582254485346 1107 40-41 -0.7970921911825002 1107 42-43 -0.8576499956450334 1107 44-45 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2311 26-27 0.9287063680177141 2311 28-29 0.9011798369246762 2311 30-31 0.9259773050473896 2311 32-33 0.9629118165613733 2311 34-35 0.9546211631141617 2311 36-37 0.986957607526108 2311 38-39 0.9876214440168027 2311 40-41 1.0461883812146269 2311 42-43 1.0280847990707613 2311 44-45 1.0408116584826033 2311 46-47 1.053621071947326 2311 48-49 1.0145129399066022 2311 50-51 1.0093696654516222 2311 52-53 0.8727579373321817 2311 54-55 0.8363834230152278 2311 56-57 0.8718410177858118 2311 58-59 0.834470037118578 2311 60-61 0.8317089807913973 2311 62-63 0.8112132382035355 2311 64-65 0.8318521368318272 2311 66-67 0.8036416088507679 2311 68-69 0.8576679218498668 2311 70-71 0.8138594823415701 2311 72-73 0.7482827702330823 2311 74-75 0.7856754634772258 2311 76-77 0.8146886642013698 2311 78-79 0.8275306859849536 2311 80-81 0.8356302838557887 2311 82-83 0.8558272760143311 2311 84-85 0.973888722043533 2311 86-87 0.9743018377836457 2311 88-89 0.9692756119923231 2311 90-91 0.9550181181371151 2311 92-93 0.9591363048360826 2311 94-95 1.0224057862615723 2311 96-97 1.0673556967993854 2311 98-99 1.1002903325779378 2311 100 1.1864391106723886 2312 1 0.48862845414145895 2312 2 0.5191707504146876 2312 3 0.5225201645568127 2312 4 0.4275401923719713 2312 5 0.41713425665023607 2312 6 0.4410422207533742 2312 7 0.49003434599939055 2312 8 0.46365968696174775 2312 9 0.5976267950678391 2312 10-11 0.5929575651821182 2312 12-13 0.5961793631977415 2312 14-15 0.7432242996225042 2312 16-17 0.7243828559468994 2312 18-19 0.7469534610420467 2312 20-21 0.7652942093156412 2312 22-23 0.7586076335447416 2312 24-25 0.7823600940935691 2312 26-27 0.8279660159431117 2312 28-29 0.8669908363877781 2312 30-31 0.8996065515400247 2312 32-33 0.8414345811354735 2312 34-35 0.860485086220713 2312 36-37 0.8378495028044952 2312 38-39 0.8997191491546701 2312 40-41 0.9312201245863534 2312 42-43 0.9382062832582747 2312 44-45 0.9236681724579583 2312 46-47 0.9496337314051289 2312 48-49 0.955979494331693 2312 50-51 0.9915226530623684 2312 52-53 0.8800525014622167 2312 54-55 0.8294319502592273 2312 56-57 0.7506523141150439 2312 58-59 0.7455146440384723 2312 60-61 0.8153852875979339 2312 62-63 0.8297313501675134 2312 64-65 0.828861806243637 2312 66-67 0.8041795211590639 2312 68-69 0.8370717063684765 2312 70-71 0.8273821367393879 2312 72-73 0.8634676797742884 2312 74-75 0.8716384118855345 2312 76-77 0.8662637255931358 2312 78-79 0.8546323432394729 2312 80-81 0.8659639683709308 2312 82-83 0.8687740052546218 2312 84-85 0.9017088668696438 2312 86-87 0.946639175923103 2312 88-89 0.9994067916924365 2312 90-91 1.0087007175947846 2312 92-93 1.0030667992966578 2312 94-95 1.0233987957189257 2312 96-97 1.0263926032511073 2312 98-99 1.1493091823164363 2312 100 1.2013676306601937 2313 1 0.4904939781789466 2313 2 0.47648305598140084 2313 3 0.5187140598325151 2313 4 0.41020333912481277 2313 5 0.448066489556588 2313 6 0.47665633669721075 2313 7 0.5045691577162543 2313 8 0.4772887646262163 2313 9 0.6098008620744579 2313 10-11 0.6275237145469816 2313 12-13 0.6545285379836088 2313 14-15 0.8411627618521109 2313 16-17 0.7637074639183297 2313 18-19 0.7475228005962791 2313 20-21 0.7740538239566277 2313 22-23 0.7294102931741193 2313 24-25 0.7294747730547329 2313 26-27 0.8172488615460267 2313 28-29 0.8096068140341401 2313 30-31 0.8705163544482488 2313 32-33 0.8677741114060638 2313 34-35 0.8405654794295003 2313 36-37 0.9363556051592781 2313 38-39 0.9916060105366356 2313 40-41 0.9666808868693906 2313 42-43 0.754225467892816 2313 44-45 0.9463209278624944 2313 46-47 0.9908611275787536 2313 48-49 1.0116394370972088 2313 50-51 0.9143027890401143 2313 52-53 0.889434578224936 2313 54-55 0.8122373735652957 2313 56-57 0.8900629607457731 2313 58-59 0.8465628545251462 2313 60-61 0.846321143898372 2313 62-63 0.8704351196675972 2313 64-65 0.8449676036451166 2313 66-67 0.8885877455750659 2313 68-69 0.8528022161206792 2313 70-71 0.8830246009210398 2313 72-73 0.9025968754169895 2313 74-75 0.9282906187002808 2313 76-77 0.9378393718518936 2313 78-79 0.9043165739105348 2313 80-81 0.9155359750866161 2313 82-83 0.890737576134768 2313 84-85 0.9844731358153673 2313 86-87 1.035246403376533 2313 88-89 1.0192041320472107 2313 90-91 1.0643747929180414 2313 92-93 1.0833012083271996 2313 94-95 1.1363858475751911 2313 96-97 1.2151945711864371 2313 98-99 1.1987094630462707 2313 100 1.2781215077116812 2314 1 0.31632204721429247 2314 2 0.24181874947048954 2314 3 0.33183064035958054 2314 4 0.28639107803201114 2314 5 0.22590961629907724 2314 6 0.26341941139179426 2314 7 0.3184199773988823 2314 8 0.3867057106276022 2314 9 0.41429995797498975 2314 10-11 0.46267960512773953 2314 12-13 0.5167643640844801 2314 14-15 0.594962037291431 2314 16-17 0.6091441943731439 2314 18-19 0.6196525907944803 2314 20-21 0.6041426465635098 2314 22-23 0.6561107104892727 2314 24-25 0.6721943702476452 2314 26-27 0.707606296506107 2314 28-29 0.7009072861208665 2314 30-31 0.7133861610533572 2314 32-33 0.8081651534023138 2314 34-35 0.766108432687723 2314 36-37 0.7735783547886967 2314 38-39 0.8314949344560105 2314 40-41 0.8065923529495862 2314 42-43 0.728847483063717 2314 44-45 0.8238385533490131 2314 46-47 0.7223241269777745 2314 48-49 0.6451886305590548 2314 50-51 0.80205567869681 2314 52-53 0.7230206297430328 2314 54-55 0.7446580989104348 2314 56-57 0.706013759518342 2314 58-59 0.7049025063615133 2314 60-61 0.5470851072928511 2314 62-63 0.6451128979494314 2314 64-65 0.6819521162417459 2314 66-67 0.7376529610378455 2314 68-69 0.7724715413115746 2314 70-71 0.7539840195035197 2314 72-73 0.7780069053886791 2314 74-75 0.77793701213443 2314 76-77 0.7923319086768217 2314 78-79 0.7966758606797271 2314 80-81 0.9033512088665496 2314 82-83 0.8170391182940868 2314 84-85 0.8781149565866748 2314 86-87 0.8837823211816165 2314 88-89 0.7691619433709924 2314 90-91 0.7778109342406303 2314 92-93 0.9986833067277239 2314 94-95 1.0084707947555458 2314 96-97 0.989837671920764 2314 98-99 0.9666149760304741 2314 100 0.9815008750798455 2315 1 0.30682681636959686 2315 2 0.3218446649393236 2315 3 0.37868222467643164 2315 4 0.25511826341775645 2315 5 0.28100136276226806 2315 6 0.25819597693600826 2315 7 0.26020676827626943 2315 8 0.2625535043585714 2315 9 0.13319495941343718 2315 10-11 0.31538149081657707 2315 12-13 0.27159312462071483 2315 14-15 0.4600836544139142 2315 16-17 0.3295509054896897 2315 18-19 0.4579845492447845 2315 20-21 0.4190312775709444 2315 22-23 0.36407356290362713 2315 24-25 0.5228539977212279 2315 26-27 0.631075151093178 2315 28-29 0.489071376510708 2315 30-31 0.5298198560515672 2315 32-33 0.6161486485650229 2315 34-35 0.5278228131552041 2315 36-37 0.6806938056973166 2315 38-39 0.7022470724735186 2315 40-41 0.7605281618467146 2315 42-43 0.7399926818933054 2315 44-45 0.7456526248990443 2315 46-47 0.7968056810807198 2315 48-49 0.7259738680512058 2315 50-51 0.6926743491893959 2315 52-53 0.3803707916908792 2315 54-55 0.5550714661539828 2315 56-57 0.5776137541095352 2315 58-59 0.5957719496244707 2315 60-61 0.6585410265088427 2315 62-63 0.6547380658586661 2315 64-65 0.6287315614558509 2315 66-67 0.526196582419935 2315 68-69 0.5857314711756345 2315 70-71 0.5508134051730735 2315 72-73 0.6456153777654876 2315 74-75 0.39677673798764346 2315 76-77 0.5804198423641509 2315 78-79 0.6085560895728293 2315 80-81 0.6610594962777476 2315 82-83 0.5899474245320988 2315 84-85 0.593695017815417 2315 86-87 0.6423260699827296 2315 88-89 0.5887500999549289 2315 90-91 0.6911295808703244 2315 92-93 0.8391923275177398 2315 94-95 0.7695108208842463 2315 96-97 0.7537951169145352 2315 98-99 0.9434569025055382 2315 100 0.8624782985961446 2316 1 0.06646163616057876 2316 2 0.1444509856832248 2316 3 0.13654935431767967 2316 4 -0.010472286004336695 2316 5 0.029496687237362096 2316 6 -0.07952860399739592 2316 7 -0.07449623872353328 2316 8 0.009493393020981955 2316 9 0.04641107173785741 2316 10-11 0.0065881375856804425 2316 12-13 0.03800512033267722 2316 14-15 -0.12358371470680396 2316 16-17 0.01984843514084389 2316 18-19 0.10985933454693964 2316 20-21 0.2014301887778558 2316 22-23 0.11017220463256194 2316 24-25 0.067323421944117 2316 26-27 0.0040656415697171155 2316 28-29 0.03170312975781542 2316 30-31 -0.5871796211172509 2316 32-33 -0.3816040062132231 2316 34-35 -0.5103199051403422 2316 36-37 -0.23418863032522097 2316 38-39 -0.12434053478271068 2316 40-41 0.08362040965027262 2316 42-43 0.1308078958396166 2316 44-45 0.03161622563919053 2316 46-47 -0.03651139217814858 2316 48-49 0.0031976616382323186 2316 50-51 0.16567957733848715 2316 52-53 -0.21325440458488032 2316 54-55 -0.11869789473338699 2316 56-57 -0.08901725427375595 2316 58-59 -0.4376926835961612 2316 60-61 0.007476111077032499 2316 62-63 -0.06059838586411814 2316 64-65 -0.3521037445139257 2316 66-67 -0.6143093968478368 2316 68-69 -0.6037558032953569 2316 70-71 -0.28406835561540333 2316 72-73 -0.592213795947103 2316 74-75 -0.4000589530522092 2316 76-77 -0.0823492484895354 2316 78-79 -0.04196499101434625 2316 80-81 -0.10978647789349694 2316 82-83 0.11647345414137789 2316 84-85 0.1402329949403942 2316 86-87 0.24500317436709196 2316 88-89 0.26808357013318584 2316 90-91 -0.4118223692909986 2316 92-93 -0.44572655701580643 2316 94-95 0.08591051536609484 2316 96-97 0.2972524435478263 2316 98-99 0.3551975093991615 2316 100 0.21899171667246264 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 259.0 1 688.0 2 420.0 3 367.0 4 360.0 5 348.0 6 360.0 7 815.0 8 2172.0 9 3946.0 10 6805.0 11 11763.0 12 18091.0 13 23988.0 14 28148.0 15 32379.0 16 37573.0 17 44065.0 18 52741.0 19 62389.0 20 74578.0 21 89088.0 22 99726.0 23 104069.0 24 105595.0 25 109415.0 26 119767.0 27 135918.0 28 158662.0 29 187582.0 30 226157.0 31 278286.0 32 348738.0 33 451863.0 34 618970.0 35 854058.0 36 1254066.0 37 1791827.0 38 1599427.0 39 355868.0 40 271.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.5738019239491 18.679450651505476 13.2704712535273 23.476276171018128 2 15.958659847608775 19.749272807532353 40.45648842509565 23.835578919763226 3 19.121750260573336 25.49653173472682 29.29801225843751 26.083705746262336 4 12.961715103430771 16.212539486082967 37.486369726012384 33.33937568447388 5 13.66476676703852 37.368416010309815 34.1565777220559 14.810239500595761 6 31.380025697062152 36.21881717066797 17.98406730463918 14.417089827630706 7 28.18191283603483 32.12150061917268 21.738651524706665 17.95793502008583 8 26.4542408998376 35.08416597797003 19.86239130687561 18.599201815316757 9 27.03490678622547 15.983084632282923 20.31087586443375 36.67113271705785 10-11 25.46958263818968 25.945194400494042 27.650203784395078 20.9350191769212 12-13 25.747533647398324 23.597374161997806 27.94454772240077 22.7105444682031 14-15 23.353188495098525 25.00798148542993 25.636068272100314 26.002761747371224 16-17 22.62332448828377 27.896073646396264 26.478769835416614 23.001832029903344 18-19 22.445366281160435 27.078719065655203 28.504648899358543 21.971265753825815 20-21 23.29639107433503 26.347150420859002 28.378904676933704 21.977553827872267 22-23 23.277335223679373 26.039166751057113 28.16828257550711 22.515215449756404 24-25 22.80811773782708 26.164500049755297 28.4020559490747 22.625326263342927 26-27 22.692291994821105 26.334005337625893 28.22819163720254 22.745511030350464 28-29 22.539510357134088 26.549548333077063 28.378209294522673 22.532732015266184 30-31 23.144176697227277 26.307870751722977 28.283338275663127 22.264614275386617 32-33 22.949989992033466 26.475276015840905 28.07476871583954 22.499965276286094 34-35 23.069854371203093 26.506499736821265 28.17931729008111 22.24432860189453 36-37 23.010563806781875 26.342833025314267 28.059426056313384 22.58717711159047 38-39 22.964595847882233 26.547865016925698 28.084680065598853 22.402859069593212 40-41 22.94440745806862 26.17600746894468 28.3214015255018 22.558183547484898 42-43 22.739906909258035 26.34598189131093 27.992894490823623 22.92121670860741 44-45 22.57119788628099 26.595518362180066 28.117292667108373 22.715991084430573 46-47 22.660033987937574 26.391405761063645 27.76727055624893 23.181289694749854 48-49 22.85842835099776 26.50285727392952 27.718634317235345 22.920080057837378 50-51 22.822171632836497 26.641164145973526 27.57435417501882 22.96231004617116 52-53 22.7025803104428 26.878160637308866 27.37732022379796 23.041938828450373 54-55 22.661340436696157 26.652082634093365 27.56662121539563 23.11995571381485 56-57 22.832767858753307 26.707651122778653 27.27976979356207 23.179811224905972 58-59 22.67075841161717 26.65452955099976 27.631204117344378 23.04350792003868 60-61 22.869043061343145 26.836176457714444 27.451294662520294 22.843485818422117 62-63 22.832938240368573 26.747826019536912 27.47115627525865 22.948079464835867 64-65 22.611612893499704 26.515122432898742 27.499479762141597 23.373784911459957 66-67 22.730144858183788 26.492020125904276 27.346948950212656 23.43088606569928 68-69 22.39902160526582 26.641592986347582 27.52120706876627 23.43817833962033 70-71 22.539619688039174 26.373860859260965 27.608600793912146 23.477918658787704 72-73 22.658090892281642 26.644256953179962 27.300874229741257 23.39677792479714 74-75 22.66721152295678 26.7876172255347 27.232132738190902 23.31303851331762 76-77 22.6206381396632 26.56443293682215 27.26774365506106 23.54718526845359 78-79 22.5279242373334 26.495809749037242 27.383085428997266 23.593180584632083 80-81 22.52200754729091 26.7747530018393 27.20643676234864 23.49680268852114 82-83 22.911742080317563 26.487476331834998 27.45345619817339 23.147325389674048 84-85 22.863055637517256 26.66175434893573 27.086250751116175 23.38893926243084 86-87 22.53194873514232 26.837524399254587 27.316703603296023 23.31382326230707 88-89 22.666690287326478 26.397460427272968 27.396652029934554 23.539197255465997 90-91 22.909252575616662 26.344549736720847 27.39895336687845 23.347244320784043 92-93 22.92992697593137 26.42983814679205 27.25958685591537 23.380648021361214 94-95 22.681560785939894 26.230568936686975 27.38633248693376 23.701537790439364 96-97 22.76274121250287 26.16541682970176 27.353847051921683 23.71799490587369 98-99 22.666847049764247 26.28166292894146 27.24993131960235 23.80155870169194 100 22.939732445940674 26.163215735127082 26.968992264769465 23.92805955416278 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2305.0 1 1724.0 2 2239.0 3 3028.5 4 2151.5 5 1774.0 6 2586.5 7 4696.5 8 7642.5 9 9116.5 10 8083.5 11 6884.5 12 7064.0 13 7699.5 14 8467.0 15 9398.5 16 10111.5 17 10702.5 18 11542.0 19 12337.5 20 13076.0 21 14309.0 22 16154.5 23 18735.0 24 21868.0 25 25837.5 26 31973.0 27 39872.5 28 48796.0 29 60993.0 30 77569.5 31 94491.0 32 115654.5 33 141090.5 34 165943.0 35 188862.5 36 215671.0 37 245918.0 38 261821.0 39 268579.0 40 271561.5 41 271909.0 42 278579.0 43 292182.0 44 308770.5 45 328910.5 46 346289.0 47 359195.5 48 373239.5 49 382838.5 50 382255.5 51 377183.5 52 368563.5 53 357294.5 54 345360.5 55 321508.0 56 291462.0 57 259956.0 58 229267.0 59 200143.5 60 166670.0 61 134918.5 62 108969.0 63 85953.5 64 64735.5 65 47764.5 66 35489.0 67 26711.5 68 20562.0 69 15359.5 70 11479.5 71 8713.0 72 6405.5 73 4670.0 74 3474.5 75 2444.5 76 1644.0 77 1057.5 78 685.5 79 447.0 80 319.0 81 237.0 82 164.5 83 134.5 84 111.5 85 75.5 86 60.0 87 49.5 88 44.5 89 40.5 90 37.5 91 28.5 92 18.0 93 13.5 94 11.5 95 8.0 96 6.0 97 5.5 98 2.0 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02450598432477995 2 0.1115522738367783 3 0.016326560483395338 4 0.017499662060646553 5 0.019576805220366592 6 0.04482539513074594 7 0.04536351512031071 8 0.03801279606285586 9 0.03242711057117347 10-11 0.036161663298753026 12-13 0.035483632111901406 14-15 0.03146387578985252 16-17 0.03820651925909918 18-19 0.0364630104929093 20-21 0.04748370787919594 22-23 0.047564425877630656 24-25 0.06633943231354573 26-27 0.05873041566109978 28-29 0.05032498142409796 30-31 0.048323175062916994 32-33 0.04312493596372124 34-35 0.04513212352479786 36-37 0.04049891041464513 38-39 0.04575096151279735 40-41 0.05548555212402417 42-43 0.05696000089543166 44-45 0.06032863203010717 46-47 0.06098513841737619 48-49 0.05996271043720312 50-51 0.05461917894082489 52-53 0.0645797799476689 54-55 0.051003012610949576 56-57 0.05553936412298065 58-59 0.05319316096847822 60-61 0.04964156903735069 62-63 0.055813805317658685 64-65 0.05370975615846041 66-67 0.05241288698360929 68-69 0.05786404247790049 70-71 0.058568979664230345 72-73 0.06032325083021152 74-75 0.05118059220750596 76-77 0.051837098594774984 78-79 0.0620775219961927 80-81 0.06198604159796668 82-83 0.05243441178319189 84-85 0.05841830606715221 86-87 0.056045196913171544 88-89 0.06405242235789542 90-91 0.06997174224310798 92-93 0.07457804935378247 94-95 0.07730631770087588 96-97 0.0731520313814358 98-99 0.0824776508005934 100 0.12901964869805096 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 9291608.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.63497341184684 #Duplication Level Percentage of deduplicated Percentage of total 1 73.4324869953117 40.11981974554644 2 15.054729775214362 16.450295219827513 3 5.178950947784828 8.488555420004493 4 2.3105468845995536 5.049466704276886 5 1.1650937019317809 3.1827431713676533 6 0.708100991318634 2.3212247300157576 7 0.44658100573837284 1.7079258962326278 8 0.30371098542640107 1.327459329092578 9 0.2242787240177553 1.1028115911197698 >10 1.0685517120008259 10.384081615871619 >50 0.06261138037871665 2.3466291410240934 >100 0.04042369642816937 4.253603806147916 >500 0.0025264612367349943 0.9271231927658153 >1k 0.0012919028073105619 1.11596547848872 >5k 2.8708951196857072E-5 0.09169525742994135 >10k+ 8.612685359057123E-5 1.1305997007881632 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 31966 0.34403087172855334 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 25220 0.27142772273647364 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14895 0.1603059448913471 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.0762399791295543E-5 2 0.0 0.0 0.0 0.0 1.0762399791295543E-5 3 0.0 0.0 0.0 0.0 1.0762399791295543E-5 4 0.0 0.0 0.0 0.0 1.0762399791295543E-5 5 0.0 0.0 0.0 0.0 1.0762399791295543E-5 6 0.0 0.0 0.0 0.0 2.1524799582591086E-5 7 0.0 0.0 0.0 1.0762399791295543E-5 2.1524799582591086E-5 8 0.0 0.0 0.0 1.0762399791295543E-5 7.53367985390688E-5 9 0.0 0.0 0.0 1.0762399791295543E-5 1.1838639770425098E-4 10-11 0.0 0.0 0.0 2.1524799582591086E-5 1.1838639770425098E-4 12-13 0.0 0.0 0.0 2.1524799582591086E-5 1.1838639770425098E-4 14-15 0.0 0.0 0.0 2.6905999478238854E-5 1.4529239718248982E-4 16-17 0.0 0.0 0.0 4.843079906082994E-5 1.9372319624331977E-4 18-19 0.0 0.0 0.0 6.995559864342103E-5 2.260103956172064E-4 20-21 0.0 0.0 0.0 1.2376759759989873E-4 2.260103956172064E-4 22-23 0.0 0.0 0.0 4.089711920692306E-4 2.6905999478238856E-4 24-25 0.0 1.0762399791295543E-5 0.0 0.0010493339796513155 3.174907938432185E-4 26-27 0.0 1.0762399791295543E-5 0.0 0.0020879055595113354 3.228719937388663E-4 28-29 0.0 1.0762399791295543E-5 0.0 0.005671784690012751 3.336343935301618E-4 30-31 0.0 1.6143599686943313E-5 0.0 0.01839832244321973 3.4977799321710514E-4 32-33 0.0 2.1524799582591086E-5 0.0 0.048231694664690974 3.8744639248663955E-4 34-35 0.0 4.304959916518217E-5 0.0 0.09411180497498388 4.304959916518217E-4 36-37 0.0 4.843079906082994E-5 0.0 0.16635441357405523 4.304959916518217E-4 38-39 0.0 7.53367985390688E-5 0.0 0.27172906993062984 4.304959916518217E-4 40-41 0.0 1.0762399791295543E-4 0.0 0.3881620920727607 4.520207912344128E-4 42-43 0.0 1.291487974955465E-4 0.0 0.5044013910186482 4.950703903995949E-4 44-45 0.0 1.291487974955465E-4 0.0 0.644866851894742 5.381199895647771E-4 46-47 0.0 1.291487974955465E-4 0.0 0.8069378303518616 5.811695887299593E-4 48-49 0.0 1.5605479697378537E-4 0.0 0.9799326445971461 6.34981587686437E-4 50-51 0.0 1.6143599686943314E-4 0.0 1.158314039937974 6.34981587686437E-4 52-53 0.0 1.6143599686943314E-4 0.0 1.3392138368299653 7.156995861211535E-4 54-55 0.0 1.7219839666072868E-4 0.0 1.546685998806665 7.964175845558702E-4 56-57 0.0 1.7219839666072868E-4 0.0 1.778588808309606 8.017987844515179E-4 58-59 0.0 1.7219839666072868E-4 0.0 2.0360038865178125 8.23323584034109E-4 60-61 5.381199895647771E-6 1.8296079645202423E-4 0.0 2.2957813114802086 8.394671837210524E-4 62-63 1.0762399791295543E-5 1.9372319624331977E-4 0.0 2.5624843407083038 8.71754383094939E-4 64-65 1.0762399791295543E-5 1.9372319624331977E-4 0.0 2.8344555646342373 8.825167828862345E-4 66-67 1.0762399791295543E-5 1.9372319624331977E-4 0.0 3.1410870970880387 8.932791826775301E-4 68-69 1.0762399791295543E-5 1.9372319624331977E-4 0.0 3.4744201434240445 8.932791826775301E-4 70-71 1.0762399791295543E-5 1.9910439613896755E-4 0.0 3.8216366854908213 8.986603825731779E-4 72-73 1.0762399791295543E-5 2.0448559603461532E-4 0.0 4.159985010129571 0.0010439527797556677 74-75 1.0762399791295543E-5 2.0448559603461532E-4 0.0 4.502498383487551 0.0010439527797556677 76-77 1.0762399791295543E-5 2.0448559603461532E-4 0.0 4.878994034186547 0.001060096379442611 78-79 1.0762399791295543E-5 2.1524799582591086E-4 0.0 5.283062953150843 0.0010762399791295542 80-81 1.0762399791295543E-5 2.260103956172064E-4 0.0 5.708436042502008 0.0010977647787121453 82-83 1.0762399791295543E-5 2.421539953041497E-4 0.0 6.138259384166874 0.0011623391774599186 84-85 1.0762399791295543E-5 2.4753519519979747E-4 0.0 6.56841636022527 0.0011623391774599186 86-87 1.0762399791295543E-5 2.4753519519979747E-4 0.0 7.007850524903763 0.0011623391774599186 88 2.1524799582591086E-5 2.4753519519979747E-4 0.0 7.354539709380766 0.0011838639770425097 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 14895 0.0 52.51328 1 GTATCAA 27145 0.0 41.94907 1 CAACGCA 33820 0.0 32.970284 5 ATCAACG 33805 0.0 32.96995 3 TATCAAC 34705 0.0 32.43035 2 TCAACGC 34990 0.0 31.961313 4 AACGCAG 36185 0.0 31.12672 6 TACCTGG 7450 0.0 30.555552 2 GTACATG 37210 0.0 28.440697 1 ACGCAGA 40405 0.0 27.46933 7 TACATGG 38510 0.0 27.015474 2 CGCAGAG 41585 0.0 26.55313 8 ACATGGG 39340 0.0 25.858784 3 GTGGTAT 7170 0.0 25.452473 1 GTACCTG 9640 0.0 24.249165 1 CATGGGG 27475 0.0 23.92608 4 GCAGAGT 45255 0.0 23.850895 9 ACCTGGG 9440 0.0 23.543644 3 ATGGGGG 18480 0.0 22.045284 5 TGGTATC 7850 0.0 21.928679 2 >>END_MODULE