FastQCFastQC Report
Mon 6 Jun 2016
ERR1211335_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211335_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9291608
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT277670.2988395550049034No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT231690.24935404076452647No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141820.1526323538401534No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA147500.052.8785741
GTATCAA264050.040.379681
CAACGCA332250.031.1889515
ATCAACG334150.031.003413
TCAACGC343100.030.3317434
TATCAAC347400.030.2241482
AACGCAG355500.029.5846256
TACCTGG74700.029.0793172
GTACATG368950.026.1769241
ACGCAGA403800.025.7091377
TACATGG379000.025.3026222
CGCAGAG417650.024.867818
ACATGGG385850.023.9187073
GTACCTG93750.023.6271571
ACCTGGG89450.023.3941023
GTGGTAT72800.022.9487651
GCAGAGT465400.022.0641449
CATGGGG265450.021.9530774
TGGTATC72900.021.4952642
GTACTAG29900.020.717791
Produced by FastQC (version 0.11.5)