FastQCFastQC Report
Mon 6 Jun 2016
ERR1211334_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211334_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2172424
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91220.41989961443990675No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81980.3773664809447879No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50320.2316306577353224No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38600.056.6684881
TACCTGG22000.046.611142
GTATCAA70350.042.861751
ACCTGGG25250.040.0076143
GTACCTG28850.036.0348241
ATCAACG86500.034.5467033
CAACGCA87350.034.2089545
TATCAAC90150.033.4463122
TCAACGC89400.033.425294
AACGCAG91500.032.6558956
CCTGGGG24850.030.0625864
GTACATG85000.029.7741451
ACGCAGA102450.028.939637
TACATGG87350.028.8640862
ACATGGG86850.028.4024033
CGCAGAG103750.028.3971658
CATGGGG60550.026.3827674
GTGGTAT16400.026.1019841
TGGGGGG48800.025.9937866
ATGGGGG44850.025.8749855
Produced by FastQC (version 0.11.5)