##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211334_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2172424 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.33998519626003 31.0 31.0 34.0 28.0 34.0 2 31.330854382017506 31.0 31.0 34.0 28.0 34.0 3 31.37608956630934 31.0 31.0 34.0 28.0 34.0 4 35.00112731216374 37.0 35.0 37.0 32.0 37.0 5 35.05732490526711 37.0 35.0 37.0 32.0 37.0 6 35.124681461814085 37.0 35.0 37.0 32.0 37.0 7 35.05339979672476 37.0 35.0 37.0 32.0 37.0 8 35.06023778047011 37.0 35.0 37.0 32.0 37.0 9 36.6159584869252 39.0 37.0 39.0 32.0 39.0 10-11 36.53894451543529 39.0 37.0 39.0 32.0 39.0 12-13 36.44676982946238 39.0 37.0 39.0 32.0 39.0 14-15 37.56415851601713 40.0 37.0 41.0 32.0 41.0 16-17 37.55217236598381 40.0 37.0 41.0 32.0 41.0 18-19 37.570689929774296 40.0 37.0 41.0 32.0 41.0 20-21 37.5431421306338 40.0 37.0 41.0 32.0 41.0 22-23 37.4152858742124 40.0 37.0 41.0 31.0 41.0 24-25 37.30262508607896 40.0 37.0 41.0 31.0 41.0 26-27 37.15915539507941 40.0 36.0 41.0 31.0 41.0 28-29 37.014474844689616 39.5 36.0 41.0 30.0 41.0 30-31 36.8144296417274 39.0 36.0 41.0 30.0 41.0 32-33 36.64357901588272 39.0 35.0 41.0 30.0 41.0 34-35 36.469820808460966 39.0 35.0 41.0 29.5 41.0 36-37 36.36039649718471 39.0 35.0 41.0 29.0 41.0 38-39 36.20515838528759 39.0 35.0 40.5 28.5 41.0 40-41 36.0306022212975 39.0 35.0 40.0 28.0 41.0 42-43 35.881240494489106 39.0 35.0 40.0 27.0 41.0 44-45 35.73293772302276 38.5 35.0 40.0 26.5 41.0 46-47 35.540301064617225 38.0 34.0 40.0 26.0 41.0 48-49 35.290441690940625 38.0 34.0 40.0 24.5 41.0 50-51 34.618967337867744 37.5 33.5 39.5 24.0 40.5 52-53 34.86136914340847 38.0 33.5 40.0 24.5 40.5 54-55 35.23398332922119 38.0 34.0 40.0 25.0 41.0 56-57 35.14372148346732 38.0 34.0 40.0 25.0 41.0 58-59 34.93699503411857 38.0 34.0 40.0 25.0 41.0 60-61 34.68374635890599 38.0 33.5 40.0 24.0 41.0 62-63 34.4047883838514 37.0 33.0 40.0 23.5 41.0 64-65 34.14288370962574 37.0 33.0 40.0 23.0 41.0 66-67 33.92693576392085 36.0 33.0 39.5 23.0 41.0 68-69 33.592956301348174 36.0 32.5 39.0 22.5 41.0 70-71 33.24853596719609 35.0 32.0 39.0 22.0 40.5 72-73 32.882901542240376 35.0 32.0 38.0 21.5 40.0 74-75 32.51532343594069 35.0 31.0 37.0 20.5 39.5 76-77 32.15269279845923 35.0 31.0 37.0 20.0 39.0 78-79 31.77842746167415 35.0 31.0 36.0 20.0 39.0 80-81 31.443287314078653 35.0 30.5 36.0 19.0 38.0 82-83 31.120363934480565 34.0 30.0 35.5 18.5 37.0 84-85 30.80559135785648 34.0 30.0 35.0 18.0 37.0 86-87 30.53721396007409 34.0 29.5 35.0 18.0 36.0 88-89 30.26547372888534 34.0 29.0 35.0 17.0 36.0 90-91 29.989759365575047 34.0 29.0 35.0 15.5 36.0 92-93 29.738745290974506 34.0 29.0 35.0 14.0 35.0 94-95 29.49975235036991 34.0 28.5 35.0 11.5 35.0 96-97 29.279769971239503 34.0 27.0 35.0 10.5 35.0 98-99 29.034029959160826 34.0 27.0 35.0 10.0 35.0 100 27.364745556116116 32.0 24.0 34.0 8.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.2994356146509176 1101 2 -0.3596507717521291 1101 3 -0.39037862560625314 1101 4 -0.24697628314373077 1101 5 -0.23284638903346178 1101 6 -0.35714074202238777 1101 7 -0.2602170276321729 1101 8 -0.5356627368333591 1101 9 -0.5054182307789787 1101 10-11 -0.39107496912338746 1101 12-13 -0.4087250557398292 1101 14-15 -0.4893724195437912 1101 16-17 -0.4534547864856435 1101 18-19 -0.5734626337228548 1101 20-21 -0.6099134132659998 1101 22-23 -0.5586704565580618 1101 24-25 -0.5688705038371822 1101 26-27 -0.7280938055525468 1101 28-29 -0.7483666258235999 1101 30-31 -0.6546953402174722 1101 32-33 -0.762597716655165 1101 34-35 -0.7512094713526594 1101 36-37 -0.8716158875557198 1101 38-39 -1.0535245983087123 1101 40-41 -0.8055412787333367 1101 42-43 -0.8093050649227536 1101 44-45 -0.9883923066741858 1101 46-47 -0.9561823681950088 1101 48-49 -1.0809963952500894 1101 50-51 -1.0368531374213106 1101 52-53 -0.7177996284634958 1101 54-55 -0.5833932379946702 1101 56-57 -0.5643317014321951 1101 58-59 -0.8009709330834411 1101 60-61 -0.8401408098326257 1101 62-63 -0.5879692022735696 1101 64-65 -0.6665482064407655 1101 66-67 -0.74046130002111 1101 68-69 -0.6397543359611007 1101 70-71 -0.6462300006872681 1101 72-73 -0.779375157506017 1101 74-75 -0.8390916594526914 1101 76-77 -1.044639737500539 1101 78-79 -0.9833382876251484 1101 80-81 -1.0595672949796722 1101 82-83 -0.9516060839385219 1101 84-85 -1.0025154832058583 1101 86-87 -0.9958639414859505 1101 88-89 -1.0310173960608573 1101 90-91 -0.9739141449540831 1101 92-93 -1.026297645616669 1101 94-95 -1.1861503146851398 1101 96-97 -1.1890088588780934 1101 98-99 -1.0130790607294813 1101 100 -1.051326711923359 1102 1 -0.3902810031970496 1102 2 -0.34354264924145994 1102 3 -0.4284904018149511 1102 4 -0.23841658438240643 1102 5 -0.2701468788985082 1102 6 -0.22417094092382683 1102 7 -0.18086715924799535 1102 8 -0.2798070961881933 1102 9 -0.3264066280673461 1102 10-11 -0.21228632468210407 1102 12-13 -0.36370142001934624 1102 14-15 -0.46057910461676954 1102 16-17 -0.5201914338731584 1102 18-19 -0.5197237184699688 1102 20-21 -0.6539776852750876 1102 22-23 -0.5366712436497565 1102 24-25 -0.6141446613504584 1102 26-27 -0.5797343710947729 1102 28-29 -0.7600186455407183 1102 30-31 -0.6610747643542325 1102 32-33 -0.6071905957722805 1102 34-35 -0.5229603828630331 1102 36-37 -0.7983286159309912 1102 38-39 -0.8737502516790414 1102 40-41 -0.7917201629313197 1102 42-43 -0.72821042556847 1102 44-45 -0.7244309997344587 1102 46-47 -0.7445876193522949 1102 48-49 -0.7782617875632312 1102 50-51 -0.7094603556953132 1102 52-53 -0.6242046254238502 1102 54-55 -0.46699692354388134 1102 56-57 -0.5261327938664664 1102 58-59 -0.47463276235192353 1102 60-61 -0.6210689961375309 1102 62-63 -0.5970833872278618 1102 64-65 -0.6315970474712103 1102 66-67 -0.5241349662911645 1102 68-69 -0.4654323603303254 1102 70-71 -0.5140734766312107 1102 72-73 -0.7020812216340815 1102 74-75 -0.6117092906129429 1102 76-77 -0.6819292019566099 1102 78-79 -0.6415334588022787 1102 80-81 -0.6818877760550173 1102 82-83 -0.7998048843127705 1102 84-85 -0.7571759388921393 1102 86-87 -0.7972791752322799 1102 88-89 -0.9040956443071231 1102 90-91 -0.9140582715502781 1102 92-93 -0.960694318393255 1102 94-95 -0.8948861137286848 1102 96-97 -0.8119792265601724 1102 98-99 -0.8852379123718848 1102 100 -0.8963256936043358 1103 1 -0.3540594052199637 1103 2 -0.397875498558637 1103 3 -0.37754476090065836 1103 4 -0.23223203399722792 1103 5 -0.36706953130705244 1103 6 -0.5735636589685882 1103 7 -0.360362063507786 1103 8 -0.36069750497828323 1103 9 -0.5644846768037013 1103 10-11 -0.6147326418619699 1103 12-13 -0.6734315018497412 1103 14-15 -0.750412503541078 1103 16-17 -0.6010176087432271 1103 18-19 -0.6406146700819235 1103 20-21 -0.7801154625100395 1103 22-23 -0.8049758752772718 1103 24-25 -0.7419285458741527 1103 26-27 -0.789956611411057 1103 28-29 -0.8233582665605823 1103 30-31 -0.8275265640348763 1103 32-33 -1.1515622116172466 1103 34-35 -1.2007983233968957 1103 36-37 -1.21033854957102 1103 38-39 -0.9933804995224591 1103 40-41 -1.103420688111477 1103 42-43 -1.0655574742007872 1103 44-45 -1.06212952552076 1103 46-47 -0.8795114207337846 1103 48-49 -1.0232865990457256 1103 50-51 -0.9983302458191261 1103 52-53 -1.0732834114932288 1103 54-55 -0.8845269031428273 1103 56-57 -0.8855116247186601 1103 58-59 -0.8054008100478711 1103 60-61 -0.7444501430296455 1103 62-63 -0.8849445333962223 1103 64-65 -0.9604652186591309 1103 66-67 -0.8982437206507043 1103 68-69 -0.7958489308722818 1103 70-71 -0.96730982579232 1103 72-73 -0.9683434860154705 1103 74-75 -0.9335917968214673 1103 76-77 -0.8531732139529709 1103 78-79 -0.7175158532799735 1103 80-81 -0.7469074307298449 1103 82-83 -0.9333200629690168 1103 84-85 -1.036445572080268 1103 86-87 -1.0504589804497009 1103 88-89 -1.0975826866969918 1103 90-91 -0.9583190612656161 1103 92-93 -1.167171992582528 1103 94-95 -1.3031437912653594 1103 96-97 -1.297019056114106 1103 98-99 -1.2813847818729869 1103 100 -1.322496944853956 1104 1 -0.43715966566012554 1104 2 -0.4995494130404303 1104 3 -0.49723896926481714 1104 4 -0.4914812686159209 1104 5 -0.48240302102477983 1104 6 -0.3972342412598593 1104 7 -0.37526669910207744 1104 8 -0.49712484337607066 1104 9 -0.5486718087740812 1104 10-11 -0.49494219694836517 1104 12-13 -0.5196485032391536 1104 14-15 -0.6655529186814917 1104 16-17 -0.697771429634976 1104 18-19 -0.7015354132686724 1104 20-21 -0.6851811138811144 1104 22-23 -0.764845234161406 1104 24-25 -0.7387858210565597 1104 26-27 -0.8152698313055566 1104 28-29 -0.8577538108083473 1104 30-31 -0.868222594878695 1104 32-33 -0.9774342049769302 1104 34-35 -0.8726617637332481 1104 36-37 -0.8425134354404875 1104 38-39 -0.9368144601977946 1104 40-41 -0.7840356396623847 1104 42-43 -0.6979458849143185 1104 44-45 -0.8695391383490971 1104 46-47 -0.9309017381339473 1104 48-49 -0.7347152907699837 1104 50-51 -0.8743595257892878 1104 52-53 -0.6897386584903487 1104 54-55 -0.6012816864000712 1104 56-57 -0.6910626691859534 1104 58-59 -0.6999105149024061 1104 60-61 -0.7347994215562679 1104 62-63 -0.676559322547952 1104 64-65 -0.7855673196233042 1104 66-67 -0.6643618389855774 1104 68-69 -0.7453217350248877 1104 70-71 -0.5601239629414323 1104 72-73 -0.659532428435945 1104 74-75 -0.7138205992177618 1104 76-77 -0.6044318075760984 1104 78-79 -0.6986023639231327 1104 80-81 -0.5936236592194746 1104 82-83 -0.5854971141608445 1104 84-85 -0.717565765515733 1104 86-87 -0.9660264452782492 1104 88-89 -0.8038897895558144 1104 90-91 -0.7460392775572586 1104 92-93 -0.7416350227105788 1104 94-95 -0.8899641958197542 1104 96-97 -0.8459102306210475 1104 98-99 -1.0639834092991975 1104 100 -0.9072723227327835 1105 1 -0.3584276913550255 1105 2 -0.5017337879245325 1105 3 -0.4592212630777226 1105 4 -0.3892485175028213 1105 5 -0.2982192595739974 1105 6 -0.3931732910012897 1105 7 -0.4254174450624504 1105 8 -0.36278863047951404 1105 9 -0.5929307794243002 1105 10-11 -0.4837034541672409 1105 12-13 -0.47167130368409715 1105 14-15 -0.5532684967747272 1105 16-17 -0.5932640211958571 1105 18-19 -0.7298632552097075 1105 20-21 -0.6526213670726051 1105 22-23 -0.6728896569128011 1105 24-25 -0.5985564792900036 1105 26-27 -0.7746374535584053 1105 28-29 -0.7581276098962135 1105 30-31 -0.7412039972370792 1105 32-33 -0.7136270147045849 1105 34-35 -0.8087152650812541 1105 36-37 -0.910829631352577 1105 38-39 -0.8138091675080759 1105 40-41 -0.7588381755838327 1105 42-43 -0.9507617631459127 1105 44-45 -1.055999993533618 1105 46-47 -1.1158448466599395 1105 48-49 -1.0104103911269746 1105 50-51 -0.9844346673107438 1105 52-53 -0.6466265591000777 1105 54-55 -0.8006155725272492 1105 56-57 -0.7577694824377375 1105 58-59 -0.8291749390452523 1105 60-61 -0.8462047063412044 1105 62-63 -0.8486281819274382 1105 64-65 -0.711799390707931 1105 66-67 -0.753209372109211 1105 68-69 -0.7901084586601854 1105 70-71 -0.6875262948002003 1105 72-73 -0.7271034896512205 1105 74-75 -0.8042149805366847 1105 76-77 -0.7242559639090089 1105 78-79 -0.7036450511400894 1105 80-81 -0.7072510832082877 1105 82-83 -0.782129565541652 1105 84-85 -0.8864061139436963 1105 86-87 -0.8147400084622873 1105 88-89 -0.9555311297641502 1105 90-91 -0.8885369149185429 1105 92-93 -1.0069822933789645 1105 94-95 -1.0026477825259583 1105 96-97 -0.9699718414168217 1105 98-99 -1.043528954287062 1105 100 -0.8991087325722944 1106 1 -0.5172221917942643 1106 2 -0.5381518981538633 1106 3 -0.5929118399980986 1106 4 -0.38532041133841943 1106 5 -0.4089887245014552 1106 6 -0.2934647833130555 1106 7 -0.4687305562768529 1106 8 -0.2939452156522506 1106 9 -0.443599901842326 1106 10-11 -0.5864808113690216 1106 12-13 -0.5711699358472089 1106 14-15 -0.5804955704257893 1106 16-17 -0.7020230297348604 1106 18-19 -0.7941990528679526 1106 20-21 -0.746652629112603 1106 22-23 -0.7577624972697805 1106 24-25 -0.7523095919574914 1106 26-27 -0.8049325812377646 1106 28-29 -0.9597505058708649 1106 30-31 -0.9489020140658369 1106 32-33 -1.0062638971660647 1106 34-35 -0.9701562123663052 1106 36-37 -0.9767385010926617 1106 38-39 -0.9087223575285535 1106 40-41 -0.8862380551473663 1106 42-43 -0.82864165176737 1106 44-45 -1.0152488751476483 1106 46-47 -1.1335670648016176 1106 48-49 -1.1144912834917164 1106 50-51 -0.9901744911319739 1106 52-53 -0.8155383061103052 1106 54-55 -0.8241859330650243 1106 56-57 -0.9021611921025965 1106 58-59 -0.8534384323975175 1106 60-61 -0.8760989094545764 1106 62-63 -0.822413760999595 1106 64-65 -0.9209585813600256 1106 66-67 -1.0691354110726081 1106 68-69 -0.974456199203658 1106 70-71 -0.8792085419293585 1106 72-73 -0.8076987406573153 1106 74-75 -0.9124435329948284 1106 76-77 -0.7650208707059818 1106 78-79 -0.6626977130591207 1106 80-81 -0.8594703677329463 1106 82-83 -0.725602914001577 1106 84-85 -0.6592212275270555 1106 86-87 -0.5839737148693658 1106 88-89 -0.7458055536140726 1106 90-91 -0.5826712453307685 1106 92-93 -0.7140652675286141 1106 94-95 -0.9137910194562018 1106 96-97 -0.8039840330237631 1106 98-99 -0.7668971024742959 1106 100 -0.728361535928606 1107 1 -0.48221917217199106 1107 2 -0.5713180436449363 1107 3 -0.5810926418270412 1107 4 -0.27102708076282767 1107 5 -0.4325952428694393 1107 6 -0.40713260277428276 1107 7 -0.42669405072531674 1107 8 -0.4919601465925112 1107 9 -0.5698718204596105 1107 10-11 -0.5346891437192411 1107 12-13 -0.6220897283542328 1107 14-15 -0.8209821287140286 1107 16-17 -0.7382357808241053 1107 18-19 -0.7812313691014765 1107 20-21 -0.8091153858495232 1107 22-23 -0.7371369459941874 1107 24-25 -0.7503543677097539 1107 26-27 -0.9117315758014399 1107 28-29 -0.9387763049412357 1107 30-31 -0.7345072010891442 1107 32-33 -0.68275768146313 1107 34-35 -0.841237041354276 1107 36-37 -0.7070564876496874 1107 38-39 -0.8374962955025467 1107 40-41 -0.8235005705153142 1107 42-43 -0.8306877407917597 1107 44-45 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2311 22-23 0.8133264964479139 2311 24-25 0.8385385206824125 2311 26-27 0.9129041100700732 2311 28-29 0.9590821619223888 2311 30-31 0.9535706456711424 2311 32-33 1.0380033829439625 2311 34-35 1.0554037642385339 2311 36-37 1.0184830234699547 2311 38-39 0.9549631633271645 2311 40-41 1.1110148838599585 2311 42-43 1.1609406873204264 2311 44-45 1.1745451515162841 2311 46-47 1.156728208777615 2311 48-49 1.0890855616843353 2311 50-51 1.157446425593598 2311 52-53 0.9713174911102769 2311 54-55 1.0072343141820213 2311 56-57 1.0102176220685521 2311 58-59 0.9852169089452829 2311 60-61 1.0230206813066118 2311 62-63 1.1124257167886569 2311 64-65 1.168096735763477 2311 66-67 1.1216833104429114 2311 68-69 0.9745430519323577 2311 70-71 0.9748132276854307 2311 72-73 1.0059341999393965 2311 74-75 0.9702079879222723 2311 76-77 0.9903591977387549 2311 78-79 1.0760370558000822 2311 80-81 1.065677292182734 2311 82-83 0.9634306856458963 2311 84-85 1.0165094194266722 2311 86-87 1.0415548733601412 2311 88-89 1.0849317389293702 2311 90-91 1.1604030242325187 2311 92-93 1.2993472186115191 2311 94-95 1.330470668351193 2311 96-97 1.2672517060789197 2311 98-99 1.2663314767991167 2311 100 1.3708650444119677 2312 1 0.5173893632960045 2312 2 0.6310683488013389 2312 3 0.6381066899090975 2312 4 0.4790794546142436 2312 5 0.48754136900503653 2312 6 0.4975199686005709 2312 7 0.5189644303768901 2312 8 0.579621609002622 2312 9 0.5930082488326889 2312 10-11 0.6576694377920731 2312 12-13 0.5887645890449065 2312 14-15 0.8492777790760329 2312 16-17 0.862392180685994 2312 18-19 0.8616732206660487 2312 20-21 0.7919194521820287 2312 22-23 0.875127452849604 2312 24-25 0.9385611753586645 2312 26-27 0.987100272458278 2312 28-29 0.9181540043542711 2312 30-31 1.02102897817079 2312 32-33 0.8480557016920329 2312 34-35 0.892394655251465 2312 36-37 0.9023486018515854 2312 38-39 0.8557322928131583 2312 40-41 0.9601426465525407 2312 42-43 0.8557905887951804 2312 44-45 0.9937379221573721 2312 46-47 0.9214986058927863 2312 48-49 0.9522142047466744 2312 50-51 0.8211793869857686 2312 52-53 0.739224205478699 2312 54-55 0.760582171895166 2312 56-57 0.7301816198508533 2312 58-59 0.8036516192655512 2312 60-61 0.8846378831120134 2312 62-63 0.8047484278111554 2312 64-65 0.8571341960129146 2312 66-67 0.8062611557185733 2312 68-69 0.925733681028845 2312 70-71 0.871264091887106 2312 72-73 0.8400030572465624 2312 74-75 0.7812332959710133 2312 76-77 0.8892331764227066 2312 78-79 0.812373575202745 2312 80-81 0.9045438096684677 2312 82-83 0.8116646858614693 2312 84-85 0.7682461165814125 2312 86-87 0.8997028761664794 2312 88-89 0.8749792040260012 2312 90-91 0.900147587917008 2312 92-93 0.9746376147714528 2312 94-95 1.0062027016116133 2312 96-97 1.000652960412193 2312 98-99 1.2449495931821914 2312 100 1.4582830502970197 2313 1 0.6126840712396486 2313 2 0.5187193479276182 2313 3 0.618696397657132 2313 4 0.5708476153388489 2313 5 0.3991882895325958 2313 6 0.5104115199770618 2313 7 0.5048458218944205 2313 8 0.5597847032730243 2313 9 0.6199752505596408 2313 10-11 0.6348356810536657 2313 12-13 0.7217049867331227 2313 14-15 0.7687052724827765 2313 16-17 0.8718003429754688 2313 18-19 0.7872703087651445 2313 20-21 0.8815812476720879 2313 22-23 0.8057731109241786 2313 24-25 0.8333503432264138 2313 26-27 0.9445135975781085 2313 28-29 0.9155519415818603 2313 30-31 0.9767097682067885 2313 32-33 0.8412890344250812 2313 34-35 1.0592307977962605 2313 36-37 1.013608868984896 2313 38-39 0.9519749272103724 2313 40-41 1.0451200770229292 2313 42-43 0.8809395153996533 2313 44-45 1.0014577942105873 2313 46-47 0.900385430367109 2313 48-49 1.104259993351313 2313 50-51 1.0823298647990711 2313 52-53 0.8959798192960093 2313 54-55 0.8629389784914707 2313 56-57 0.94937680452108 2313 58-59 0.8553229256901531 2313 60-61 0.8276096899846479 2313 62-63 0.8797929676243754 2313 64-65 0.9236185221592237 2313 66-67 0.9257590372442266 2313 68-69 0.9328673476630058 2313 70-71 0.9858883646415393 2313 72-73 0.9739062849159552 2313 74-75 0.9171303361925283 2313 76-77 0.8019447677033966 2313 78-79 0.7757428777859623 2313 80-81 0.8582607494526258 2313 82-83 0.9520934851898204 2313 84-85 0.905191837312902 2313 86-87 0.852918684834993 2313 88-89 0.8538925754088673 2313 90-91 1.026399872348346 2313 92-93 1.063419170662474 2313 94-95 1.0863000030174526 2313 96-97 1.076664366578651 2313 98-99 1.050414623607118 2313 100 1.0679568679660214 2314 1 0.30609766331698296 2314 2 0.23704492576354852 2314 3 0.335422185883008 2314 4 0.21696856587139735 2314 5 0.2277078798188228 2314 6 0.17691445216548374 2314 7 0.24266891152302605 2314 8 0.3288618451401675 2314 9 0.44506385697829387 2314 10-11 0.4423492259693944 2314 12-13 0.45772163207634264 2314 14-15 0.5464080074002524 2314 16-17 0.5887342283597192 2314 18-19 0.6119188819368446 2314 20-21 0.5652182970745514 2314 22-23 0.5438414985895221 2314 24-25 0.6605909065782996 2314 26-27 0.6815324649525678 2314 28-29 0.7416600039080592 2314 30-31 0.7104709774388027 2314 32-33 0.7184319948193547 2314 34-35 0.8413664349318992 2314 36-37 0.727583766135858 2314 38-39 0.8908855427619216 2314 40-41 0.7903672472508063 2314 42-43 0.7000595066050579 2314 44-45 0.8650084646918828 2314 46-47 0.5381321594988648 2314 48-49 0.5812451005490615 2314 50-51 0.6524100182671262 2314 52-53 0.6812948408892154 2314 54-55 0.6382172031015614 2314 56-57 0.597673247236429 2314 58-59 0.6390786990387127 2314 60-61 0.4144829273083346 2314 62-63 0.47258782558231616 2314 64-65 0.6763981869747582 2314 66-67 0.5167971786215304 2314 68-69 0.5330887915854916 2314 70-71 0.6101011105431411 2314 72-73 0.7244434959676269 2314 74-75 0.7518856288146409 2314 76-77 0.8033714593488099 2314 78-79 0.6841446733888468 2314 80-81 0.6045020941747907 2314 82-83 0.6870852842941417 2314 84-85 0.6315990402532634 2314 86-87 0.6715341106575039 2314 88-89 0.6439148746782415 2314 90-91 0.5826154862099102 2314 92-93 0.7128335377328021 2314 94-95 0.7167839818897797 2314 96-97 0.63724705987973 2314 98-99 0.707615134519429 2314 100 0.6359924156069852 2315 1 0.2664164623265428 2315 2 0.20566515971921717 2315 3 0.2837962230047708 2315 4 0.15899400737946223 2315 5 0.14626950847993214 2315 6 0.07095452789978651 2315 7 0.16317705778828184 2315 8 0.14890249959995572 2315 9 0.1185181441301566 2315 10-11 0.23557555159990073 2315 12-13 0.19731333750909386 2315 14-15 0.42709795794996097 2315 16-17 0.20190494576954876 2315 18-19 0.45750168924969614 2315 20-21 0.25646357984402357 2315 22-23 0.2756675355469227 2315 24-25 0.2926583473501623 2315 26-27 0.424011691609806 2315 28-29 0.33995820902573115 2315 30-31 0.37378585898829186 2315 32-33 0.3717653702975454 2315 34-35 0.3845651748390537 2315 36-37 0.43619110089235136 2315 38-39 0.5463819550374609 2315 40-41 0.5293518512563153 2315 42-43 0.5216361973584682 2315 44-45 0.5650386509443734 2315 46-47 0.4762086041358984 2315 48-49 0.47057548872329136 2315 50-51 0.5693719623250004 2315 52-53 0.15475134064597285 2315 54-55 0.312738066385279 2315 56-57 0.2667797657715951 2315 58-59 0.2430106416109865 2315 60-61 0.4373490494826271 2315 62-63 0.45307976236158964 2315 64-65 0.4846503268079374 2315 66-67 0.23690725715176342 2315 68-69 0.3537381279045988 2315 70-71 0.21824526376343556 2315 72-73 0.3118913165661681 2315 74-75 0.11257284090660846 2315 76-77 0.20801961063121865 2315 78-79 0.2929475794298604 2315 80-81 0.4841859560381323 2315 82-83 0.464991116979256 2315 84-85 0.49981902260414657 2315 86-87 0.4900200509901609 2315 88-89 0.4376639641338578 2315 90-91 0.6706121477626823 2315 92-93 0.7423628333399783 2315 94-95 0.769111010065437 2315 96-97 0.5669638809354502 2315 98-99 0.754164462246731 2315 100 0.7187278906460719 2316 1 0.048312756086009045 2316 2 0.03884025523410628 2316 3 0.0922664391152459 2316 4 -0.2385516675429784 2316 5 -0.09813035622673283 2316 6 -0.15322664083908677 2316 7 -0.1417699650378097 2316 8 -0.1172220047412722 2316 9 -0.027819060095808368 2316 10-11 -0.11489324206561946 2316 12-13 -0.050043949639139385 2316 14-15 -0.1031321763515578 2316 16-17 0.011329533722509666 2316 18-19 -0.043135006043883095 2316 20-21 -0.011263576902123873 2316 22-23 0.01505528799629019 2316 24-25 -0.16080369832009467 2316 26-27 -0.21130693295316405 2316 28-29 -0.16913050502920868 2316 30-31 -0.63682702433929 2316 32-33 -0.35297909681823114 2316 34-35 -0.4984746838545817 2316 36-37 -0.2079798699842641 2316 38-39 -0.26779033260385887 2316 40-41 -0.1314489959941767 2316 42-43 -0.06002313526787617 2316 44-45 -0.22302701040352702 2316 46-47 -0.224118142830811 2316 48-49 -0.004354413335967422 2316 50-51 0.15478294408611504 2316 52-53 -0.2332545717970831 2316 54-55 -0.24455341926156393 2316 56-57 -0.3316089776421052 2316 58-59 -0.705284444793925 2316 60-61 -0.23563483823406983 2316 62-63 -0.1919148928735268 2316 64-65 -0.4702683544945998 2316 66-67 -0.8044118867703389 2316 68-69 -1.0625962393433355 2316 70-71 -0.653624700353312 2316 72-73 -0.9715930773702723 2316 74-75 -0.9060620640390837 2316 76-77 -0.5181130401319862 2316 78-79 -0.4419756059991471 2316 80-81 -0.4535762842929003 2316 82-83 -0.22822863268286042 2316 84-85 -0.15258561297274653 2316 86-87 -0.08922234024323927 2316 88-89 0.0491555759753588 2316 90-91 -0.6063200702200859 2316 92-93 -0.6981877127641631 2316 94-95 -0.1845900877376856 2316 96-97 -0.09672233403571084 2316 98-99 0.004484823187194564 2316 100 -0.013021284327372484 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 64.0 1 163.0 2 104.0 3 94.0 4 80.0 5 88.0 6 94.0 7 259.0 8 723.0 9 1194.0 10 1632.0 11 2730.0 12 4070.0 13 5343.0 14 6132.0 15 7287.0 16 8974.0 17 11290.0 18 14002.0 19 16698.0 20 19692.0 21 23220.0 22 25592.0 23 26173.0 24 25632.0 25 26080.0 26 28608.0 27 31946.0 28 36742.0 29 43128.0 30 52510.0 31 63989.0 32 79783.0 33 102505.0 34 140839.0 35 189661.0 36 277469.0 37 407339.0 38 390990.0 39 99364.0 40 141.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.97475479068688 18.41563151240113 12.081997662787582 23.527616034124406 2 16.46327470127141 20.412989682170295 39.93871052473929 23.185025091819007 3 18.726545547964598 26.510649719686064 30.31690645014408 24.445898282205256 4 12.361667362636503 16.308345790963198 38.077530272385864 33.25245657401443 5 12.48908240647933 37.77222619458156 34.87810279504207 14.860588603897046 6 29.947028444003145 36.777513017223356 18.89899113640613 14.376467402367371 7 26.67670346108217 32.96640237567147 22.91064888843039 17.446245274815965 8 26.0685506245962 35.066217282206416 20.992851692536664 17.872380400660724 9 27.42797912382314 15.004176524707805 20.86291513136022 36.70492922010884 10-11 25.033955495793986 25.769406553025025 28.99980083740389 20.196837113777104 12-13 25.11787375500955 24.05957718791142 28.809018038748334 22.0135310183307 14-15 22.751627293590655 25.422755587317607 26.728780717814217 25.09683640127752 16-17 21.74569254817915 27.86987121910022 27.744454265868747 22.639981966851888 18-19 21.50736043268085 27.0705282219953 29.795482776921382 21.626628568402463 20-21 22.523754566237383 26.60469970138696 29.550740269285964 21.320805463089695 22-23 22.320119739796212 26.468873409705836 29.33505267399689 21.875954176501065 24-25 21.86494910251423 26.348082652938317 29.730239525302267 22.05672871924519 26-27 21.730990133394005 26.607940816945025 29.529832412321145 22.13123663733982 28-29 21.580480269844948 27.12837024097312 29.522972420936377 21.76817706824555 30-31 22.55397225777363 26.54654000489097 29.62310952602233 21.27637821131307 32-33 22.285649154478136 26.7490467155436 29.343661349150796 21.621642780827468 34-35 22.440225255387993 26.848736438485364 29.381155585193348 21.329882720933295 36-37 22.169920692685828 26.83938835532016 29.183102197823203 21.807588754170812 38-39 22.051891490107863 27.167147962152466 29.168532785237517 21.61242776250215 40-41 22.104834581221503 26.54824989757975 29.566785947780527 21.78012957341822 42-43 21.829748672664195 26.87467255595767 29.0087038224738 22.286874948904337 44-45 21.55247498849662 27.210410754727672 29.238414058120743 21.99870019865497 46-47 21.57528276045041 26.810626095235417 28.919765376362562 22.69432576795161 48-49 21.828667655120732 26.943641076023912 28.87013620491675 22.35755506393861 50-51 21.90808041915528 27.1418333621957 28.719008264462808 22.23107795418621 52-53 21.76650310156467 27.478660765241468 28.374330550745945 22.380505582447913 54-55 21.617544380006336 27.145494204254877 28.769168699291836 22.467792716446947 56-57 22.00980176538633 27.190261150884133 28.278169136529456 22.52176794720008 58-59 21.730920245116213 27.25540768647969 28.663190886208874 22.35048118219522 60-61 22.170881334926523 27.369618235497168 28.420819448765283 22.03868098081103 62-63 21.97077898301244 27.32464925706195 28.428589654367194 22.275982105558416 64-65 21.724496225142868 27.0197051595241 28.431177482765353 22.824621132567678 66-67 21.70312728123516 27.051597538188883 28.237022319432104 23.00825286114385 68-69 21.448422869781457 27.097171192657086 28.468325436512927 22.986080501048534 70-71 21.547920680533863 26.81209551388017 28.71901540173523 22.920968403850733 72-73 21.632443437246664 27.249175510354483 28.224952096690988 22.893428955707865 74-75 21.829969605437167 27.3130575493264 28.114521572908842 22.742451272327592 76-77 21.684455256484537 26.986650958299442 28.225435027509597 23.10345875770642 78-79 21.597227516000903 27.00336864728326 28.39516095786853 23.004242878847304 80-81 21.676281807692575 27.186344887623758 28.06534150447593 23.072031800207732 82-83 22.079275364054308 27.057347822122086 28.302418615704745 22.560958198118865 84-85 21.99903598653513 27.264101690754337 27.901234909078514 22.835627413632018 86-87 21.66798238018563 27.373373704398325 28.163999314662174 22.794644600753873 88-89 21.73330281073517 26.925514573696184 28.232635627886538 23.108546987682104 90-91 22.242804680455606 26.720859718762703 28.17140481611097 22.864930784670715 92-93 22.163084343876267 26.83553207852419 28.113942118225747 22.88744145937379 94-95 21.942356146613136 26.655520035489864 28.09759528976135 23.304528528135645 96-97 22.01229758912712 26.58609694347605 28.071403038097838 23.330202429298996 98-99 21.928655044579077 26.602858976420286 27.961768826385985 23.506717152614648 100 22.184238835357764 26.533303344023167 27.85489168358943 23.42756613702964 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 436.0 1 357.0 2 627.5 3 959.0 4 748.0 5 654.0 6 968.5 7 1700.5 8 2677.5 9 3219.0 10 2919.5 11 2465.0 12 2549.0 13 2808.5 14 3021.0 15 3276.0 16 3535.0 17 3705.5 18 3958.5 19 4306.5 20 4654.0 21 5276.5 22 6092.0 23 7096.5 24 8337.5 25 9861.5 26 11904.0 27 14383.5 28 16928.5 29 20139.0 30 24612.5 31 28818.0 32 34030.0 33 39827.0 34 45005.5 35 50034.5 36 55452.0 37 61277.0 38 64172.5 39 65018.0 40 65088.0 41 65025.0 42 65765.5 43 68064.0 44 70946.5 45 75000.5 46 79137.0 47 81447.0 48 83825.5 49 86249.0 50 87283.0 51 85774.5 52 82205.0 53 77948.0 54 73535.5 55 67285.5 56 60139.5 57 52686.5 58 45395.0 59 38888.5 60 31969.5 61 25324.0 62 20260.5 63 16128.5 64 12156.0 65 8917.5 66 6682.5 67 5155.5 68 3979.5 69 3042.5 70 2358.5 71 1812.0 72 1417.0 73 1092.5 74 830.5 75 634.0 76 446.0 77 278.0 78 178.5 79 129.5 80 91.0 81 61.5 82 39.5 83 26.5 84 23.0 85 19.5 86 18.5 87 13.0 88 9.0 89 9.0 90 9.5 91 9.0 92 5.0 93 3.0 94 4.0 95 3.5 96 1.0 97 1.0 98 1.0 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.027895107032512988 2 0.1101994822373533 3 0.018182454253865728 4 0.018182454253865728 5 0.021680850515369006 6 0.049253736839585636 7 0.04971405213715186 8 0.03963314712045163 9 0.03507602567454604 10-11 0.03827521699263128 12-13 0.03779188593018674 14-15 0.033142701424767905 16-17 0.03993235206386967 18-19 0.038965689938980606 20-21 0.048425169303966446 22-23 0.04828707471469658 24-25 0.06640048167392737 26-27 0.05809179055285709 28-29 0.05077277732155418 30-31 0.049575957547882 32-33 0.04529502528051614 34-35 0.04704422341126778 36-37 0.04301646455756335 38-39 0.0472973968249292 40-41 0.05627354512747051 42-43 0.05760845949041256 44-45 0.06089971386801104 46-47 0.06317827459096383 48-49 0.06246478587973619 50-51 0.05523783570794651 52-53 0.06550286684367324 54-55 0.05413307899378759 56-57 0.05912749997238108 58-59 0.055652119475756115 60-61 0.05056563543764937 62-63 0.057401317606507754 64-65 0.055974340184052465 66-67 0.054179110523544204 68-69 0.05970289409433886 70-71 0.060071146332391835 72-73 0.06370763718316498 74-75 0.05282118039572386 76-77 0.054409268172327316 78-79 0.06327033765047707 80-81 0.06301716423681565 82-83 0.054409268172327316 84-85 0.05949575221043406 86-87 0.056710844660158424 88-89 0.06237272282022294 90-91 0.06819571133443564 92-93 0.0737655264349869 94-95 0.07657344975014085 96-97 0.07328219537254238 98-99 0.0822583436750837 100 0.1271390851877902 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2172424.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.86138304823152 #Duplication Level Percentage of deduplicated Percentage of total 1 79.21054202001298 53.75336933478139 2 14.05133575465945 19.070861559725124 3 3.5373303725829266 7.201443941459805 4 1.2842996556439068 3.48617403521452 5 0.5802768618602894 1.9689195198363412 6 0.3332842586581659 1.35702784444486 7 0.21498680688937635 1.0212511436845302 8 0.14978774879527296 0.8131843037542638 9 0.10585041352163928 0.64648399120251 >10 0.4876284563827028 5.732746894204427 >50 0.028961162039842503 1.3623126391680336 >100 0.014762353069190307 1.9070346875989692 >500 2.935802721266638E-4 0.13572382499617994 >1k 4.403704081899925E-4 0.43018226660256853 >5k 2.2018520409499624E-4 1.1132840133264572 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9122 0.41989961443990675 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8198 0.3773664809447879 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5032 0.2316306577353224 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 4.6031529756622094E-5 0.0 5 0.0 0.0 0.0 4.6031529756622094E-5 0.0 6 0.0 0.0 0.0 4.6031529756622094E-5 0.0 7 0.0 0.0 0.0 4.6031529756622094E-5 0.0 8 0.0 0.0 0.0 4.6031529756622094E-5 0.0 9 0.0 0.0 0.0 4.6031529756622094E-5 1.3809458926986628E-4 10-11 0.0 0.0 0.0 4.6031529756622094E-5 1.3809458926986628E-4 12-13 0.0 0.0 0.0 4.6031529756622094E-5 1.3809458926986628E-4 14-15 0.0 0.0 0.0 4.6031529756622094E-5 2.0714188390479944E-4 16-17 0.0 0.0 0.0 4.6031529756622094E-5 2.3015764878311047E-4 18-19 0.0 0.0 0.0 4.6031529756622094E-5 2.531734136614215E-4 20-21 0.0 0.0 0.0 4.6031529756622094E-5 2.7618917853973257E-4 22-23 0.0 0.0 0.0 1.8412611902648838E-4 3.4523647317466575E-4 24-25 0.0 0.0 0.0 3.6825223805297676E-4 4.142837678095989E-4 26-27 0.0 0.0 0.0 0.0013579301278203518 4.142837678095989E-4 28-29 0.0 0.0 0.0 0.007572186644964334 4.142837678095989E-4 30-31 0.0 0.0 0.0 0.027710980913486505 4.142837678095989E-4 32-33 0.0 0.0 0.0 0.06867904239688016 4.372995326879099E-4 34-35 0.0 0.0 0.0 0.1327089002883415 4.6031529756622094E-4 36-37 0.0 0.0 0.0 0.2408829952164034 4.6031529756622094E-4 38-39 0.0 0.0 0.0 0.41214330167591595 4.6031529756622094E-4 40-41 0.0 0.0 0.0 0.6040257334663951 4.6031529756622094E-4 42-43 0.0 0.0 0.0 0.7774725375893472 4.83331062444532E-4 44-45 0.0 0.0 0.0 0.9889183695263908 5.06346827322843E-4 46-47 0.0 0.0 0.0 1.240227506232669 5.06346827322843E-4 48-49 0.0 0.0 0.0 1.5116754372074697 5.523783570794651E-4 50-51 0.0 0.0 0.0 1.782455911000799 5.523783570794651E-4 52-53 0.0 0.0 0.0 2.048679263348223 5.753941219577762E-4 54-55 0.0 0.0 0.0 2.3611182715712955 5.984098868360873E-4 56-57 0.0 0.0 0.0 2.701820639064934 5.984098868360873E-4 58-59 0.0 0.0 0.0 3.0839053518097757 5.984098868360873E-4 60-61 0.0 0.0 0.0 3.4529171101037366 5.984098868360873E-4 62-63 0.0 0.0 0.0 3.828626455977286 6.444414165927094E-4 64-65 0.0 0.0 0.0 4.2074659458742865 6.444414165927094E-4 66-67 0.0 0.0 0.0 4.637032181563084 6.444414165927094E-4 68-69 0.0 0.0 0.0 5.129914786432114 6.444414165927094E-4 70-71 0.0 0.0 0.0 5.622498186357728 6.904729463493314E-4 72-73 0.0 0.0 0.0 6.087876952197178 8.745990653758198E-4 74-75 0.0 0.0 0.0 6.555096979226891 8.745990653758198E-4 76-77 0.0 0.0 0.0 7.061604916903882 8.745990653758198E-4 78-79 0.0 0.0 0.0 7.621440381803921 8.745990653758198E-4 80-81 0.0 0.0 0.0 8.178030623856117 8.976148302541308E-4 82-83 0.0 0.0 0.0 8.748085088362124 9.66662124889064E-4 84-85 0.0 0.0 0.0 9.320878428888651 9.66662124889064E-4 86-87 0.0 0.0 0.0 9.897446354855221 9.66662124889064E-4 88 0.0 0.0 0.0 10.341903790420286 9.66662124889064E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3860 0.0 56.668488 1 TACCTGG 2200 0.0 46.61114 2 GTATCAA 7035 0.0 42.86175 1 ACCTGGG 2525 0.0 40.007614 3 GTACCTG 2885 0.0 36.034824 1 ATCAACG 8650 0.0 34.546703 3 CAACGCA 8735 0.0 34.208954 5 TATCAAC 9015 0.0 33.446312 2 TCAACGC 8940 0.0 33.42529 4 AACGCAG 9150 0.0 32.655895 6 CCTGGGG 2485 0.0 30.062586 4 GTACATG 8500 0.0 29.774145 1 ACGCAGA 10245 0.0 28.93963 7 TACATGG 8735 0.0 28.864086 2 ACATGGG 8685 0.0 28.402403 3 CGCAGAG 10375 0.0 28.397165 8 CATGGGG 6055 0.0 26.382767 4 GTGGTAT 1640 0.0 26.101984 1 TGGGGGG 4880 0.0 25.993786 6 ATGGGGG 4485 0.0 25.874985 5 >>END_MODULE