FastQCFastQC Report
Mon 6 Jun 2016
ERR1211334_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211334_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2172424
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78880.3630967067202351No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74730.34399362187123694No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46700.21496724396342518No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40950.057.525321
TACCTGG20850.046.8011472
ACCTGGG23900.040.045293
GTATCAA69400.037.324061
TAACGCA2800.036.8624234
GTACCTG26900.036.2844281
TATAACG3650.030.8473342
GTACATG83750.030.5926481
ATAACGC3500.029.4899373
ATCAACG86500.029.4511993
CAACGCA86650.029.4475775
TACATGG88400.028.8168682
TCAACGC88850.028.7250424
ACATGGG86250.028.5030543
TATCAAC90450.028.371222
AACGCAG90850.028.1371966
GTATAAC5850.027.2729631
CCTGGGG23800.027.0061594
ATGGGGG41000.026.8845795
GTGGTAT16450.026.8145921
Produced by FastQC (version 0.11.5)