##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211334_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2172424 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.116767260903025 33.0 31.0 34.0 30.0 34.0 2 32.24282414482624 34.0 31.0 34.0 30.0 34.0 3 32.30716839806594 34.0 31.0 34.0 30.0 34.0 4 35.951521894436816 37.0 35.0 37.0 35.0 37.0 5 35.86680040360445 37.0 35.0 37.0 35.0 37.0 6 35.88484614421494 37.0 35.0 37.0 35.0 37.0 7 35.79704606467246 37.0 35.0 37.0 33.0 37.0 8 35.78273117954874 37.0 35.0 37.0 33.0 37.0 9 37.40813487606471 39.0 37.0 39.0 34.0 39.0 10-11 37.35648680920483 39.0 37.0 39.0 34.0 39.0 12-13 37.28184162023619 39.0 37.0 39.0 34.0 39.0 14-15 38.5165200255567 40.0 38.0 41.0 34.0 41.0 16-17 38.49890283848825 40.0 38.0 41.0 34.0 41.0 18-19 38.51748323531686 40.0 38.0 41.0 34.0 41.0 20-21 38.48836299911987 40.0 38.0 41.0 34.0 41.0 22-23 38.375575624279605 40.0 38.0 41.0 34.0 41.0 24-25 38.291215020640536 40.0 38.0 41.0 34.0 41.0 26-27 38.18827217891167 40.0 38.0 41.0 33.5 41.0 28-29 38.07072491373691 40.0 37.0 41.0 33.0 41.0 30-31 38.051589836974735 40.0 37.0 41.0 33.0 41.0 32-33 37.94738204880815 40.0 37.0 41.0 33.0 41.0 34-35 37.79265419641838 40.0 37.0 41.0 33.0 41.0 36-37 37.63781816993368 40.0 37.0 41.0 33.0 41.0 38-39 37.51672371507588 40.0 36.5 41.0 32.0 41.0 40-41 37.405563784970155 40.0 36.0 41.0 32.0 41.0 42-43 37.28813367003863 40.0 36.0 41.0 31.5 41.0 44-45 37.12207469628397 40.0 36.0 41.0 31.0 41.0 46-47 36.8649667376166 39.0 36.0 41.0 30.5 41.0 48-49 35.294043658144076 39.0 35.0 41.0 23.0 41.0 50-51 36.047252285925765 39.0 35.0 40.0 28.0 41.0 52-53 36.15451449624935 39.0 35.0 40.0 29.0 41.0 54-55 36.00108703457521 38.5 35.0 40.0 28.0 41.0 56-57 35.79327447128185 38.0 35.0 40.0 28.0 41.0 58-59 35.57571841408491 38.0 34.0 40.0 28.0 41.0 60-61 35.327823666098325 38.0 34.0 40.0 27.5 41.0 62-63 35.01137416084521 37.0 34.0 40.0 26.5 41.0 64-65 34.69099448358148 37.0 34.0 39.5 26.0 41.0 66-67 34.35809100801686 36.0 33.0 39.0 26.0 41.0 68-69 34.00295292263389 36.0 33.0 39.0 26.0 40.0 70-71 33.6390803544796 35.0 33.0 38.5 25.5 40.0 72-73 33.25635741457469 35.0 32.0 37.5 25.0 40.0 74-75 32.88657946146793 35.0 32.0 37.0 24.5 39.0 76-77 31.914342918325335 34.0 30.5 36.0 23.5 39.0 78-79 32.33871104351637 35.0 31.5 36.0 24.5 38.5 80-81 32.207313351353136 35.0 32.0 36.0 24.0 37.5 82-83 31.92926058633121 34.5 31.0 35.0 24.0 37.0 84-85 31.63661674700703 34.0 31.0 35.0 23.5 36.5 86-87 31.3712926205934 34.0 31.0 35.0 23.0 36.0 88-89 31.14153636674977 34.0 31.0 35.0 21.5 36.0 90-91 30.909953351647744 34.0 30.5 35.0 20.0 35.5 92-93 30.713834638173765 34.0 30.0 35.0 20.0 35.0 94-95 30.516017361251762 34.0 30.0 35.0 20.0 35.0 96-97 30.32650118024842 34.0 29.5 35.0 19.0 35.0 98-99 30.1081667298833 34.0 29.0 35.0 18.5 35.0 100 27.75732131480779 31.0 25.0 34.0 11.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.18014521551396 1101 2 -0.20141927290767114 1101 3 -0.2561535908980659 1101 4 -0.12192574757087726 1101 5 -0.14779821694184392 1101 6 -0.12659815568336086 1101 7 -0.2406931639392056 1101 8 -0.3534029530742515 1101 9 -0.3680261999021681 1101 10-11 -0.3296665625001012 1101 12-13 -0.3089986985229487 1101 14-15 -0.5209143224678812 1101 16-17 -0.498847248005049 1101 18-19 -0.43856678516694814 1101 20-21 -0.5428810236156707 1101 22-23 -0.6287294876354323 1101 24-25 -0.5728138036377928 1101 26-27 -0.627331135499098 1101 28-29 -0.621393091931111 1101 30-31 -0.5758665271777303 1101 32-33 -0.44257008804024167 1101 34-35 -0.535824299020824 1101 36-37 -0.44756931093827745 1101 38-39 -0.44966627467321985 1101 40-41 -0.3536107271989408 1101 42-43 -0.3654881860753747 1101 44-45 -0.4355313995020751 1101 46-47 -0.45415817861746177 1101 48-49 0.8183615492832033 1101 50-51 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2 0.3917659849673427 2311 3 0.411694192552865 2311 4 0.21613326890030748 2311 5 0.3117148841748545 2311 6 0.33265251725908485 2311 7 0.3980143761625996 2311 8 0.2104364053150789 2311 9 0.4385293516507147 2311 10-11 0.3877001178313719 2311 12-13 0.4318500594006309 2311 14-15 0.4860936041853847 2311 16-17 0.5229436384640991 2311 18-19 0.5890782331893405 2311 20-21 0.6067482937381925 2311 22-23 0.6706192071320984 2311 24-25 0.6357289288345882 2311 26-27 0.6494678882954545 2311 28-29 0.6255310720265399 2311 30-31 0.5534580086937595 2311 32-33 0.5782704937979091 2311 34-35 0.7116330432908526 2311 36-37 0.6955151480698021 2311 38-39 0.7541332566496664 2311 40-41 0.7734048511394036 2311 42-43 0.7778552811046922 2311 44-45 0.8186470452724066 2311 46-47 0.9313427673551615 2311 48-49 -5.942221875744195 2311 50-51 -0.9703989810118472 2311 52-53 0.4461465372710407 2311 54-55 0.8578694731192087 2311 56-57 0.9361586282613814 2311 58-59 0.8863405211462592 2311 60-61 0.7684304346350572 2311 62-63 0.7156059853052028 2311 64-65 0.7467338223034119 2311 66-67 0.8671434914686813 2311 68-69 0.8669975744266978 2311 70-71 0.9508131875865757 2311 72-73 0.43416878354108235 2311 74-75 0.7798928791181012 2311 76-77 0.9127839508520132 2311 78-79 0.8364992754458314 2311 80-81 0.722736572874588 2311 82-83 0.8465885502389234 2311 84-85 0.8801977602185254 2311 86-87 0.8550665261355306 2311 88-89 0.9107530550181906 2311 90-91 0.9440557935326872 2311 92-93 0.9314417947774736 2311 94-95 0.9788528737600686 2311 96-97 1.0314005957179297 2311 98-99 1.0339377990520902 2311 100 1.3062641457963693 2312 1 0.041485474496965935 2312 2 0.2643218132539289 2312 3 0.2273758110907096 2312 4 -0.02197512372136856 2312 5 0.22352945919959666 2312 6 0.16173363455415313 2312 7 0.2786652172577959 2312 8 0.28281486878587003 2312 9 0.278563456297249 2312 10-11 0.2730724580852453 2312 12-13 0.330183004398819 2312 14-15 0.31977794515697866 2312 16-17 0.4407147474911781 2312 18-19 0.27112856529657137 2312 20-21 0.42728259556431425 2312 22-23 0.5868194951240255 2312 24-25 0.7308796986095061 2312 26-27 0.6387595198129432 2312 28-29 0.7429186005949902 2312 30-31 0.8789171208757196 2312 32-33 0.7484113077784826 2312 34-35 0.5969932816877517 2312 36-37 0.9048829212442087 2312 38-39 0.9392481016587695 2312 40-41 0.5891892547824824 2312 42-43 0.670726093297425 2312 44-45 0.8686146445673373 2312 46-47 0.9696741891329594 2312 48-49 -4.538777590434684 2312 50-51 -0.49530492537945037 2312 52-53 0.840801485897849 2312 54-55 1.0601934814607787 2312 56-57 1.133442616568729 2312 58-59 1.1923906751141473 2312 60-61 1.1337683927064859 2312 62-63 1.0441250923624281 2312 64-65 1.1730480355633048 2312 66-67 1.1313226725311765 2312 68-69 1.1249276792642746 2312 70-71 1.2522504363923659 2312 72-73 1.333132154608542 2312 74-75 1.0140935014439947 2312 76-77 1.1436420402818293 2312 78-79 0.7058697336189113 2312 80-81 0.8949919846636689 2312 82-83 1.047468887722598 2312 84-85 1.1126211845083454 2312 86-87 1.1859926118657356 2312 88-89 1.2290278594683173 2312 90-91 1.213063292811416 2312 92-93 1.3029536362024885 2312 94-95 1.2930085288126065 2312 96-97 1.4692456196602421 2312 98-99 1.5139650556611564 2312 100 0.9057698091427149 2313 1 0.11016186116154358 2313 2 0.23702258705750268 2313 3 0.28066244256435624 2313 4 -0.020684726623080962 2313 5 0.1089036339943732 2313 6 0.2841518312645732 2313 7 0.12454504390493071 2313 8 0.2770332315464259 2313 9 0.46334266985051187 2313 10-11 0.1626803214347987 2313 12-13 -0.06185863605730191 2313 14-15 0.2040587288589819 2313 16-17 0.17518666893468549 2313 18-19 0.4909136812677559 2313 20-21 0.37613322309597663 2313 22-23 0.4012449610300379 2313 24-25 0.3152255920977183 2313 26-27 0.48767904511075244 2313 28-29 0.5115060939272098 2313 30-31 0.6636457555821877 2313 32-33 0.5035778640141544 2313 34-35 0.44870209532349037 2313 36-37 -0.23540724422841208 2313 38-39 0.24773544328549946 2313 40-41 0.3960199761691072 2313 42-43 0.48830489725207116 2313 44-45 0.4842475446013097 2313 46-47 0.3000745289333864 2313 48-49 -5.560485354613682 2313 50-51 -1.3671639293146072 2313 52-53 0.1584759208212816 2313 54-55 0.24586023113180744 2313 56-57 0.30828086672071464 2313 58-59 0.4397299297017909 2313 60-61 0.6191003718887345 2313 62-63 0.7297868741909284 2313 64-65 0.786281778143362 2313 66-67 0.8976674954493333 2313 68-69 0.8243893514276479 2313 70-71 0.8266264908945615 2313 72-73 0.5602533767971636 2313 74-75 0.7357260338576168 2313 76-77 0.7741947686519985 2313 78-79 0.634379685138974 2313 80-81 -0.18241554050000985 2313 82-83 0.20938236405114097 2313 84-85 0.5421746934507716 2313 86-87 0.7064893091819187 2313 88-89 0.680489398741905 2313 90-91 0.7533358845410767 2313 92-93 0.7457851015235306 2313 94-95 0.6163674175559137 2313 96-97 0.6554223494164404 2313 98-99 0.7636977236347811 2313 100 0.8318036016495931 2314 1 -0.01250067099444152 2314 2 0.10378149165713779 2314 3 0.15707884702809594 2314 4 0.03966481586684978 2314 5 0.20397025089791043 2314 6 0.2792865637355959 2314 7 0.07194193901333534 2314 8 0.15950244668077573 2314 9 0.3565674940422028 2314 10-11 0.2159504608515519 2314 12-13 0.19684857116495635 2314 14-15 0.08635894571732194 2314 16-17 0.36050815892552635 2314 18-19 0.4966805766160576 2314 20-21 0.5805825696207947 2314 22-23 0.2639315841711678 2314 24-25 0.43438334599067474 2314 26-27 0.35407125533793504 2314 28-29 0.43300419303824356 2314 30-31 0.34894720265079826 2314 32-33 0.37296656703362174 2314 34-35 0.25534179919841904 2314 36-37 0.33234208440961055 2314 38-39 0.3375361619760966 2314 40-41 0.18691839408408129 2314 42-43 0.20742543508263367 2314 44-45 0.48672330101663164 2314 46-47 0.5070268112907854 2314 48-49 -6.624294806552122 2314 50-51 -1.625351538962299 2314 52-53 -0.7897660415873062 2314 54-55 -0.39434836102599746 2314 56-57 -0.032452303374157054 2314 58-59 0.3020392296480736 2314 60-61 0.49612504867383933 2314 62-63 0.4416762445485958 2314 64-65 0.5295911622091793 2314 66-67 0.695250455685688 2314 68-69 0.5581305936576015 2314 70-71 0.5157783047942033 2314 72-73 0.7289846415997019 2314 74-75 0.6138838190296809 2314 76-77 0.5502515545021858 2314 78-79 0.3577011890991173 2314 80-81 0.4600212324315933 2314 82-83 0.286432975162878 2314 84-85 0.4144271808229725 2314 86-87 0.47800386778362736 2314 88-89 0.2635266181447946 2314 90-91 0.6240197854889509 2314 92-93 0.5471248949538854 2314 94-95 0.6812438514220105 2314 96-97 0.7187168349100723 2314 98-99 0.7768360109913708 2314 100 -0.52730391739194 2315 1 0.07306076025633246 2315 2 0.1885175063419382 2315 3 0.3160683015232877 2315 4 0.12082258867600615 2315 5 0.25596086255672645 2315 6 0.27916041652151335 2315 7 0.317570679429096 2315 8 0.18537699313268519 2315 9 0.37866839974471844 2315 10-11 0.24710997213598063 2315 12-13 0.3861463306007735 2315 14-15 0.24171685467351978 2315 16-17 0.3525588729790954 2315 18-19 0.35331556088789284 2315 20-21 0.301596511617781 2315 22-23 0.41100910666286694 2315 24-25 0.3843702140369558 2315 26-27 0.48892557254674074 2315 28-29 0.622227025626529 2315 30-31 0.541150083577314 2315 32-33 0.6035028277485921 2315 34-35 0.5955323878116658 2315 36-37 0.5133294291716766 2315 38-39 0.6507799528176932 2315 40-41 0.663067590894137 2315 42-43 0.725558163215851 2315 44-45 0.6601627895773277 2315 46-47 0.5858109188107363 2315 48-49 -5.010537461494092 2315 50-51 -1.022025548412131 2315 52-53 0.09928568767836055 2315 54-55 0.09897158365313174 2315 56-57 0.36433054037484425 2315 58-59 0.644825175066849 2315 60-61 0.5567963989748819 2315 62-63 0.3062840775722151 2315 64-65 0.6107316939412399 2315 66-67 -0.02760275004835222 2315 68-69 0.6081577339864666 2315 70-71 0.48022964630805376 2315 72-73 0.6212471622853997 2315 74-75 0.6991247817599273 2315 76-77 0.8036680176044122 2315 78-79 0.6852006926997802 2315 80-81 0.5522217714451401 2315 82-83 0.5040003540105538 2315 84-85 0.5559348296831423 2315 86-87 0.6333575383083705 2315 88-89 0.5393115828336299 2315 90-91 0.6731593695364744 2315 92-93 0.735442858345909 2315 94-95 0.39321233415837753 2315 96-97 0.4832033625887533 2315 98-99 0.4351809365544561 2315 100 0.5742971752325836 2316 1 0.2802471872944281 2316 2 0.2589207612938651 2316 3 0.1827834567786084 2316 4 -0.023784454545811684 2316 5 0.17252898379628334 2316 6 0.08355643265837642 2316 7 0.170151806687052 2316 8 0.29287528995688206 2316 9 0.3614104463870049 2316 10-11 0.373649506018225 2316 12-13 0.29304647434288 2316 14-15 0.4199946451197363 2316 16-17 0.3473390029432082 2316 18-19 0.07648782225042794 2316 20-21 0.3665733396672479 2316 22-23 0.48141525793200657 2316 24-25 0.5178177470387268 2316 26-27 0.6320261776085943 2316 28-29 0.6774545673709298 2316 30-31 0.535971680326881 2316 32-33 0.5536374994347852 2316 34-35 0.538927470627975 2316 36-37 0.5198811812740445 2316 38-39 0.5126543023825576 2316 40-41 0.20144739669677847 2316 42-43 0.22011534739647942 2316 44-45 0.32950706618607484 2316 46-47 0.42154325049868646 2316 48-49 -4.672515061235487 2316 50-51 -0.9202546835548588 2316 52-53 0.34760299801974526 2316 54-55 0.5260498042801629 2316 56-57 0.4374980122177021 2316 58-59 0.43598106559679906 2316 60-61 0.5109697377674252 2316 62-63 0.5247975067355029 2316 64-65 0.46997081869395885 2316 66-67 0.5451896840364157 2316 68-69 0.41909985087504964 2316 70-71 -0.1524677405356627 2316 72-73 -0.005618454529859207 2316 74-75 0.3039014587487827 2316 76-77 0.25630369452460755 2316 78-79 0.32295893470022463 2316 80-81 0.21095478948961954 2316 82-83 0.17667037984004352 2316 84-85 0.43808714375583335 2316 86-87 0.2914692609898104 2316 88-89 0.18566531726027335 2316 90-91 0.23899852305456548 2316 92-93 0.3168049299276099 2316 94-95 0.5766334894809475 2316 96-97 0.7070929922755269 2316 98-99 0.5048570039603781 2316 100 -0.09184335068775695 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 0.0 10 4.0 11 5.0 12 12.0 13 51.0 14 205.0 15 687.0 16 1724.0 17 3642.0 18 6493.0 19 8810.0 20 11438.0 21 15338.0 22 18491.0 23 19425.0 24 19462.0 25 20435.0 26 23582.0 27 28429.0 28 35055.0 29 42835.0 30 52308.0 31 64517.0 32 81089.0 33 104939.0 34 146089.0 35 201686.0 36 311554.0 37 476343.0 38 415968.0 39 61799.0 40 8.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.79640893924924 19.23948969621398 12.891221084059406 24.07288028047737 2 16.081350183622984 20.709349075164358 39.75725308485693 23.45204765635573 3 18.305613875026985 26.3613268330586 30.971114714873405 24.361944577041005 4 12.277138738343854 16.5778436896704 38.14905040372057 32.995967168265175 5 12.37831497892665 37.86316776007498 34.93688121774323 14.821636043255145 6 30.148700390623922 37.104485224325664 18.547179139228014 14.199635245822403 7 26.707263407143355 33.08447153962578 22.701691750781617 17.506573302449247 8 26.205568567661725 35.41491739767697 20.66420664206642 17.715307392594884 9 27.216571825006437 15.01189798256804 20.722100968733788 37.04942922369174 10-11 25.084301904736257 25.874648804398635 28.906640373172444 20.134408917692664 12-13 24.979573499267868 24.060553584637983 28.807166007725016 22.152706908369137 14-15 22.630085342613306 25.501767613997295 26.672604744938823 25.195542298450572 16-17 21.774602821693886 27.974329071493358 27.62444252799357 22.62662557881918 18-19 21.510359630698073 27.204393435348457 29.695459862098676 21.58978707185479 20-21 22.430295013840535 26.676106201208004 29.46587071995861 21.427728064992852 22-23 22.23279416428798 26.485361696212102 29.265191719637617 22.0166524198623 24-25 21.823903539964363 26.392097671586058 29.674492490642933 22.109506297806643 26-27 21.77633494405439 26.602260840145046 29.471339023601534 22.15006519219903 28-29 21.59364354398845 27.200930028947173 29.430486159236846 21.77494026782753 30-31 22.47073058512591 26.586343939533087 29.573807121880957 21.36911835346005 32-33 22.284268621419802 26.745992962465177 29.281613951140535 21.688124464974486 34-35 22.369950992139266 26.890557244859973 29.389799302347587 21.349692460653173 36-37 22.15250675265496 26.848183188772534 29.184798635863558 21.81451142270895 38-39 22.029729581027507 27.13628271808971 29.182076245502593 21.65191145538019 40-41 22.093988352614446 26.59456446134179 29.545493255407894 21.765953930635863 42-43 21.78863865747374 26.91975984228861 28.972033388228496 22.319568112009154 44-45 21.47687232477353 27.20888886433673 29.27601201705516 22.038226793834585 46-47 21.587590010153367 26.81587276961383 28.89433245917533 22.70220476105747 48-49 21.934982965493152 27.052441954402372 28.7625568467393 22.25001823336517 50-51 21.852706821991585 27.14324124363677 28.671682821151823 22.332369113219823 52-53 21.717474925819097 27.51967606713458 28.305567768771972 22.457281238274355 54-55 21.57404969239183 27.210939470888167 28.6903543064223 22.5246565302977 56-57 21.915618717657516 27.1350699893521 28.25712771819567 22.69218357479472 58-59 21.594823945031692 27.24472300054299 28.620749104550686 22.539703949874635 60-61 22.08915503860089 27.376306481464646 28.361831234208807 22.172707245725658 62-63 21.91032119884774 27.318854507015182 28.28497147546153 22.485852818675546 64-65 21.479122002807074 27.081141144970918 28.340526742146622 23.099210110075383 66-67 21.56236078511726 27.02769966076175 28.16782566354058 23.242113890580413 68-69 21.35105610253329 27.202263578420034 28.252691049863422 23.193989269183263 70-71 21.480002283769398 26.9061647039756 28.464008530983754 23.14982448127125 72-73 21.498555428939767 27.340400796528424 27.939132336521748 23.22191143801006 74-75 21.70781414649574 27.417047113400212 27.730550260170784 23.14458847993327 76-77 21.442058844991127 27.267261336277908 27.940816631413263 23.349863187317705 78-79 21.243108521678923 27.267259539426437 27.98559372594658 23.50403821294806 80-81 21.369342214257554 27.579698039676913 27.528993084285997 23.521966661779533 82-83 21.68208134132645 27.390586655378147 27.753389145205475 23.173942858089923 84-85 21.59155211669019 27.59455408961258 27.37122480034808 23.442668993349155 86-87 21.193117738750047 27.810637674346545 27.618481522092203 23.377763064811205 88-89 21.19318265539928 27.40551624168392 27.654929603406735 23.74637149951006 90-91 21.629191562144985 27.167618747259148 27.61048297707525 23.59270671352062 92-93 21.455342238214627 27.343009803226494 27.514384418698224 23.687263539860652 94-95 21.158474845651696 27.11928972274964 27.576229291041642 24.146006140557024 96-97 21.087723578545667 27.221423030978187 27.624721785544125 24.06613160493202 98-99 20.948814677993507 27.29035186743693 27.438735803851444 24.322097650718117 100 20.86293799161384 27.26564852735724 27.551649096503432 24.319764384525485 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 344.0 1 307.0 2 509.5 3 738.5 4 630.5 5 577.0 6 792.0 7 1465.5 8 2358.5 9 2798.5 10 2577.0 11 2298.5 12 2352.5 13 2541.0 14 2841.0 15 3110.5 16 3400.0 17 3672.5 18 3903.5 19 4243.5 20 4678.5 21 5277.0 22 6133.5 23 7175.0 24 8411.0 25 10021.5 26 12103.0 27 14379.0 28 16879.0 29 20439.5 30 24912.5 31 29134.5 32 34188.5 33 39979.0 34 45178.5 35 49956.5 36 55171.0 37 60741.0 38 63558.5 39 64569.5 40 64743.5 41 64609.0 42 65800.5 43 68216.5 44 71243.5 45 75342.5 46 79421.0 47 81741.5 48 84079.0 49 86521.5 50 87735.5 51 86917.0 52 83591.5 53 79400.5 54 74799.0 55 68127.0 56 60833.5 57 53154.0 58 45721.0 59 38996.5 60 31403.5 61 24881.5 62 20054.0 63 15467.0 64 11452.5 65 8359.5 66 6187.0 67 4840.5 68 3756.5 69 2787.0 70 2142.5 71 1635.5 72 1234.5 73 947.0 74 735.5 75 533.5 76 349.5 77 203.5 78 115.0 79 66.0 80 36.0 81 21.5 82 13.5 83 12.0 84 10.0 85 3.5 86 1.0 87 1.5 88 2.0 89 1.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.032130007770122225 2 0.0017491981307516395 3 1.3809458926986628E-4 4 0.02306179640806767 5 5.523783570794651E-4 6 9.206305951324419E-5 7 0.0 8 0.004050774618582745 9 0.009528526659620774 10-11 0.0010817409492806193 12-13 4.6031529756622094E-5 14-15 1.8412611902648838E-4 16-17 2.5317341366142156E-4 18-19 4.6031529756622094E-5 20-21 2.3015764878311047E-5 22-23 3.222207082963547E-4 24-25 4.6031529756622094E-5 26-27 6.904729463493314E-5 28-29 8.515833004975088E-4 30-31 0.002876970609788881 32-33 0.009873763132795439 34-35 0.019586415911442702 36-37 0.02303878064318936 38-39 0.022072118518300293 40-41 0.004188869207852611 42-43 0.004672200270297143 44-45 0.008193612296678733 46-47 0.01097851984695437 48-49 1.2259347162432381 50-51 0.017261823658733286 52-53 0.016985634480193555 54-55 0.008285675356191976 56-57 0.007595202409842646 58-59 0.00876900641863651 60-61 0.005961083103482562 62-63 0.007894407353260689 64-65 0.018988006024606617 66-67 0.029759383987656186 68-69 0.029529226338873074 70-71 0.026514161139814325 72-73 0.022969733348554424 74-75 0.009068211362054553 76-77 0.013510253983568584 78-79 0.00810154923716549 80-81 0.0024166553122226602 82-83 0.01406263234064805 84-85 0.02389036394368687 86-87 0.02267052840513638 88-89 0.033510953662820885 90-91 0.019632447441199324 92-93 0.012980891391367431 94-95 0.006697587579588516 96-97 0.0040968061483393665 98-99 0.016387224593357466 100 0.03452364731746657 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2172424.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.86369484073957 #Duplication Level Percentage of deduplicated Percentage of total 1 78.87720008208231 53.528982362623914 2 14.30699908561042 19.418516400652113 3 3.651355157212767 7.4338335643274345 4 1.2357284730517668 3.354444000047927 5 0.5922541489717343 2.009627741698984 6 0.3501063412428896 1.4255705942349173 7 0.20804934253952934 0.9883297965743378 8 0.14793544785591672 0.8031556871537657 9 0.10538625384619014 0.6436710514283908 >10 0.48060010733765113 5.659232052691246 >50 0.02749990415028997 1.2806220739985403 >100 0.016001422876989906 1.9791344290154569 >500 2.9474440717716644E-4 0.15496893880005352 >1k 4.421166107657444E-4 0.5517674316617542 >5k 1.4737220358858148E-4 0.7681438750911915 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7888 0.3630967067202351 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7473 0.34399362187123694 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4670 0.21496724396342518 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 6.904729463493314E-5 16-17 0.0 0.0 0.0 0.0 9.206305951324419E-5 18-19 0.0 0.0 0.0 0.0 9.206305951324419E-5 20-21 0.0 0.0 0.0 0.0 9.206305951324419E-5 22-23 0.0 0.0 0.0 9.206305951324419E-5 1.1507882439155524E-4 24-25 0.0 0.0 0.0 3.2222070829635463E-4 1.3809458926986628E-4 26-27 0.0 0.0 0.0 0.0013118985980637298 1.8412611902648838E-4 28-29 0.0 0.0 0.0 0.007503139350329402 1.8412611902648838E-4 30-31 0.0 0.0 0.0 0.02787209126763468 1.8412611902648838E-4 32-33 0.0 0.0 0.0 0.06877110545639341 1.8412611902648838E-4 34-35 0.0 0.0 0.0 0.132893026407368 1.8412611902648838E-4 36-37 0.0 0.0 0.0 0.23996236462127096 1.8412611902648838E-4 38-39 0.0 0.0 0.0 0.40457111503095156 1.8412611902648838E-4 40-41 0.0 0.0 0.0 0.5882139029949954 1.8412611902648838E-4 42-43 0.0 0.0 0.0 0.7535591578807821 1.8412611902648838E-4 44-45 0.0 0.0 0.0 0.9551542424499084 1.8412611902648838E-4 46-47 0.0 0.0 0.0 1.196037237666312 1.8412611902648838E-4 48-49 0.0 0.0 0.0 1.4591304459902856 2.3015764878311047E-4 50-51 0.0 0.0 0.0 1.7295196517806837 2.3015764878311047E-4 52-53 0.0 0.0 0.0 1.9950525311817582 2.3015764878311047E-4 54-55 0.0 0.0 0.0 2.3070542398721425 2.3015764878311047E-4 56-57 0.0 0.0 0.0 2.6481939068984692 2.531734136614215E-4 58-59 0.0 0.0 0.0 3.0298873516403795 2.7618917853973257E-4 60-61 0.0 0.0 0.0 3.3993133937021502 2.7618917853973257E-4 62-63 0.0 0.0 0.0 3.776081464760102 3.222207082963547E-4 64-65 0.0 0.0 0.0 4.155128096541007 3.222207082963547E-4 66-67 0.0 0.0 0.0 4.585476868235666 3.222207082963547E-4 68-69 0.0 0.0 0.0 5.078773756872508 3.222207082963547E-4 70-71 0.0 0.0 0.0 5.57246191351228 3.222207082963547E-4 72-73 0.0 0.0 0.0 6.038324010414174 3.6825223805297676E-4 74-75 0.0 0.0 0.0 6.505820226622427 3.6825223805297676E-4 76-77 0.0 0.0 0.0 7.012719432302349 3.6825223805297676E-4 78-79 0.0 0.0 0.0 7.5734755277975205 3.6825223805297676E-4 80-81 0.0 0.0 0.0 8.1302959274985 4.142837678095989E-4 82-83 0.0 0.0 0.0 8.701386101424031 4.6031529756622094E-4 84-85 0.0 0.0 0.0 9.276665144557416 4.6031529756622094E-4 86-87 0.0 0.0 0.0 9.854775126770832 4.6031529756622094E-4 88 0.0 0.0 0.0 10.301902391061782 4.6031529756622094E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4095 0.0 57.52532 1 TACCTGG 2085 0.0 46.801147 2 ACCTGGG 2390 0.0 40.04529 3 GTATCAA 6940 0.0 37.32406 1 TAACGCA 280 0.0 36.862423 4 GTACCTG 2690 0.0 36.284428 1 TATAACG 365 0.0 30.847334 2 GTACATG 8375 0.0 30.592648 1 ATAACGC 350 0.0 29.489937 3 ATCAACG 8650 0.0 29.451199 3 CAACGCA 8665 0.0 29.447577 5 TACATGG 8840 0.0 28.816868 2 TCAACGC 8885 0.0 28.725042 4 ACATGGG 8625 0.0 28.503054 3 TATCAAC 9045 0.0 28.37122 2 AACGCAG 9085 0.0 28.137196 6 GTATAAC 585 0.0 27.272963 1 CCTGGGG 2380 0.0 27.006159 4 ATGGGGG 4100 0.0 26.884579 5 GTGGTAT 1645 0.0 26.814592 1 >>END_MODULE