FastQCFastQC Report
Mon 6 Jun 2016
ERR1211333_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211333_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7429630
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT260850.35109419984575274No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT207620.2794486401072463No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120270.16187885534003713No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA120250.052.8907971
GTATCAA217700.041.6401021
TACCTGG56000.035.61632
ATCAACG271750.032.6971633
CAACGCA272750.032.5759625
TATCAAC277250.032.4235152
TCAACGC280800.031.7767934
AACGCAG291950.030.8844556
GTGGTAT53500.027.7857281
GTACATG293400.027.3050561
ACGCAGA325050.027.2937557
GTACCTG75750.026.7659781
CGCAGAG333700.026.4873318
TACATGG301900.026.0210192
ACCTGGG76250.025.6354853
ACATGGG303950.024.8119543
GCAGAGT363250.023.6219319
CATGGGG218000.023.1488824
TGGTATC63300.022.5169662
GTACTAG23500.022.4201581
Produced by FastQC (version 0.11.5)