##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211333_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7429630 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.369047314603822 31.0 31.0 34.0 28.0 34.0 2 31.378843226378702 31.0 31.0 34.0 28.0 34.0 3 31.429471454163934 31.0 31.0 34.0 28.0 34.0 4 35.03988045165102 37.0 35.0 37.0 32.0 37.0 5 35.08549241348492 37.0 35.0 37.0 32.0 37.0 6 35.14541787410678 37.0 35.0 37.0 32.0 37.0 7 35.08205321126355 37.0 35.0 37.0 32.0 37.0 8 35.078129866494024 37.0 35.0 37.0 32.0 37.0 9 36.63208410109252 39.0 37.0 39.0 32.0 39.0 10-11 36.560567686412384 39.0 37.0 39.0 32.0 39.0 12-13 36.48020695512428 39.0 37.0 39.0 32.0 39.0 14-15 37.612592820907636 40.0 37.0 41.0 32.0 41.0 16-17 37.60996489730982 40.0 37.0 41.0 32.0 41.0 18-19 37.6064894214113 40.0 37.0 41.0 32.0 41.0 20-21 37.57016601095882 40.0 37.0 41.0 32.0 41.0 22-23 37.449313895846764 40.0 37.0 41.0 31.0 41.0 24-25 37.338551583322456 40.0 37.0 41.0 31.0 41.0 26-27 37.19545394588963 40.0 36.5 41.0 31.0 41.0 28-29 37.05348435924803 40.0 36.0 41.0 30.0 41.0 30-31 36.86554646732071 39.0 36.0 41.0 30.0 41.0 32-33 36.736251132290576 39.0 35.5 41.0 30.0 41.0 34-35 36.59070034443169 39.0 35.0 41.0 30.0 41.0 36-37 36.495389003220886 39.0 35.0 41.0 29.5 41.0 38-39 36.341602475493396 39.0 35.0 41.0 29.0 41.0 40-41 36.17377843849559 39.0 35.0 40.0 28.5 41.0 42-43 36.021291437124056 39.0 35.0 40.0 28.0 41.0 44-45 35.88624521005757 39.0 35.0 40.0 27.0 41.0 46-47 35.71860644473547 38.5 35.0 40.0 26.5 41.0 48-49 35.48228101533993 38.0 34.0 40.0 26.0 41.0 50-51 34.81126038577965 37.5 33.5 39.5 25.0 40.5 52-53 35.0507054187086 38.0 33.5 40.0 26.0 40.5 54-55 35.41401469521362 38.0 34.0 40.0 26.0 41.0 56-57 35.32072640225691 38.0 34.0 40.0 26.0 41.0 58-59 35.102674022797906 38.0 34.0 40.0 26.0 41.0 60-61 34.83677296177602 37.5 34.0 40.0 26.0 41.0 62-63 34.5632276439069 37.0 33.0 40.0 25.0 41.0 64-65 34.288967620191045 36.5 33.0 40.0 25.0 41.0 66-67 34.064800400558305 36.0 33.0 39.0 24.5 41.0 68-69 33.72947448257854 36.0 32.5 39.0 23.5 41.0 70-71 33.38581281975011 35.0 32.0 39.0 23.5 40.5 72-73 33.02083818440488 35.0 32.0 38.0 23.0 40.0 74-75 32.65337298088869 35.0 31.5 37.0 22.0 39.5 76-77 32.29817386599333 35.0 31.0 37.0 21.5 39.0 78-79 31.93746101757423 35.0 31.0 36.0 20.5 39.0 80-81 31.60666204104377 35.0 31.0 36.0 20.0 37.5 82-83 31.29624092989826 34.0 30.5 35.0 20.0 37.0 84-85 30.995557450371013 34.0 30.0 35.0 19.5 37.0 86-87 30.7361824882262 34.0 30.0 35.0 19.0 36.0 88-89 30.48153521776993 34.0 29.0 35.0 18.0 36.0 90-91 30.22644653906049 34.0 29.0 35.0 18.0 36.0 92-93 29.990169900789137 34.0 29.0 35.0 17.0 35.0 94-95 29.770034039380157 34.0 29.0 35.0 16.5 35.0 96-97 29.564965819293825 34.0 29.0 35.0 15.0 35.0 98-99 29.328125694011682 34.0 27.0 35.0 13.0 35.0 100 27.63525155357669 32.0 25.0 34.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.4086909422095175 1101 2 -0.34162622964629463 1101 3 -0.41436994864470833 1101 4 -0.33940310303093213 1101 5 -0.3418713274011296 1101 6 -0.3809622576208511 1101 7 -0.4270659981888727 1101 8 -0.4944486620018793 1101 9 -0.5581243726098393 1101 10-11 -0.5091939204058704 1101 12-13 -0.5375988744087365 1101 14-15 -0.5749640030307006 1101 16-17 -0.6193924743657107 1101 18-19 -0.6623108931641184 1101 20-21 -0.7441838073823916 1101 22-23 -0.7299190409069638 1101 24-25 -0.7384740756380097 1101 26-27 -0.7511253848336921 1101 28-29 -0.730448747654691 1101 30-31 -0.7511268770246886 1101 32-33 -0.8056039014597474 1101 34-35 -0.8609750348671383 1101 36-37 -0.9023787099907778 1101 38-39 -0.8901797702979977 1101 40-41 -0.8605673911773124 1101 42-43 -0.9082306250409999 1101 44-45 -1.0647883264531472 1101 46-47 -0.9593139594203066 1101 48-49 -0.8920516447504951 1101 50-51 -0.857939935083067 1101 52-53 -0.6776256652729913 1101 54-55 -0.5234634324612557 1101 56-57 -0.5868927117730607 1101 58-59 -0.6875280282778675 1101 60-61 -0.6458543557992797 1101 62-63 -0.6634846838216006 1101 64-65 -0.746350441120363 1101 66-67 -0.7342687570805992 1101 68-69 -0.7673279554708117 1101 70-71 -0.8140023389644426 1101 72-73 -0.8211317280102222 1101 74-75 -0.7832807559216874 1101 76-77 -0.747468314591508 1101 78-79 -0.8335152173969931 1101 80-81 -0.9706125865739459 1101 82-83 -1.0084622888476247 1101 84-85 -1.0030442255324452 1101 86-87 -0.8929052586358317 1101 88-89 -0.9712692685593929 1101 90-91 -0.9629569540640652 1101 92-93 -0.9863112577163058 1101 94-95 -1.0114571634091014 1101 96-97 -1.0348662190007154 1101 98-99 -1.1115431294313645 1101 100 -1.1331120762748483 1102 1 -0.3844075418672652 1102 2 -0.3860704513933477 1102 3 -0.350083999385582 1102 4 -0.24665581724615748 1102 5 -0.29627135702956764 1102 6 -0.22303169490932362 1102 7 -0.24388821777261427 1102 8 -0.27212320017409297 1102 9 -0.411905690792004 1102 10-11 -0.39172594253569315 1102 12-13 -0.4477077589090399 1102 14-15 -0.48224191230886504 1102 16-17 -0.4912958181307232 1102 18-19 -0.5862833910046206 1102 20-21 -0.5832042608063759 1102 22-23 -0.6381984348747451 1102 24-25 -0.6716602653013837 1102 26-27 -0.6682917517666169 1102 28-29 -0.7582412222499428 1102 30-31 -0.6536416558407936 1102 32-33 -0.6853474303153391 1102 34-35 -0.6142811870156919 1102 36-37 -0.6958128744089933 1102 38-39 -0.8795277595402595 1102 40-41 -0.8096848777274133 1102 42-43 -0.8091329851855278 1102 44-45 -0.8423454183786347 1102 46-47 -0.826771262339463 1102 48-49 -0.8078684691268521 1102 50-51 -0.8105838699135788 1102 52-53 -0.6890180274544733 1102 54-55 -0.6503466517650551 1102 56-57 -0.5227228580525676 1102 58-59 -0.6380142505107429 1102 60-61 -0.6975784911010123 1102 62-63 -0.6708401172082716 1102 64-65 -0.7632193908467926 1102 66-67 -0.6673524563214741 1102 68-69 -0.6687198710472089 1102 70-71 -0.735468597949648 1102 72-73 -0.8093669080737698 1102 74-75 -0.749920477414399 1102 76-77 -0.6817064988912733 1102 78-79 -0.7207987364708437 1102 80-81 -0.690916702856839 1102 82-83 -0.8295989205955863 1102 84-85 -0.937571929753517 1102 86-87 -1.0557051893575782 1102 88-89 -1.051459740185262 1102 90-91 -1.0599066412771094 1102 92-93 -1.0284936752005684 1102 94-95 -1.0524634736122849 1102 96-97 -1.2003188314771158 1102 98-99 -1.1720217375920363 1102 100 -1.2109827939038738 1103 1 -0.4110594079682599 1103 2 -0.513694455539266 1103 3 -0.526118765343405 1103 4 -0.38677053260975924 1103 5 -0.3427406096229575 1103 6 -0.41879640544188135 1103 7 -0.4510654380770589 1103 8 -0.40226496939067147 1103 9 -0.5976535365850424 1103 10-11 -0.6100596491770602 1103 12-13 -0.5536610997963649 1103 14-15 -0.7350300621942338 1103 16-17 -0.6760861068515496 1103 18-19 -0.6925195895869294 1103 20-21 -0.8212783207289007 1103 22-23 -0.7717726397286313 1103 24-25 -0.8027950338943199 1103 26-27 -0.7625864191711145 1103 28-29 -0.7377214017891447 1103 30-31 -0.8380864809130415 1103 32-33 -0.8128065640650206 1103 34-35 -0.9171974254760826 1103 36-37 -0.9374906744388554 1103 38-39 -0.9164870338294406 1103 40-41 -0.8879005311921588 1103 42-43 -0.8719007788032798 1103 44-45 -0.9061842327523095 1103 46-47 -0.9578164370037072 1103 48-49 -1.0001324945293675 1103 50-51 -0.9445402543593744 1103 52-53 -0.8700380806838055 1103 54-55 -0.8354980830719825 1103 56-57 -0.7847696181779966 1103 58-59 -0.8094807142735121 1103 60-61 -0.845890853306436 1103 62-63 -0.8306193823623786 1103 64-65 -0.8855558819269262 1103 66-67 -0.923812024865228 1103 68-69 -0.8331156829702238 1103 70-71 -0.845115470506677 1103 72-73 -0.9055106580188692 1103 74-75 -0.8761173249378267 1103 76-77 -0.8297571062854061 1103 78-79 -0.9164096221331768 1103 80-81 -0.8902529392091374 1103 82-83 -1.0438136342024436 1103 84-85 -1.0063446246861893 1103 86-87 -1.037115239688287 1103 88-89 -1.125120706218624 1103 90-91 -1.0793349090399822 1103 92-93 -1.020167302964147 1103 94-95 -1.3291902648320786 1103 96-97 -1.3746039905063405 1103 98-99 -1.3893657387514864 1103 100 -1.3411678229340644 1104 1 -0.3641256123882535 1104 2 -0.3881036478385482 1104 3 -0.36885196327114755 1104 4 -0.20995216074526013 1104 5 -0.2118500419594298 1104 6 -0.27628547006091253 1104 7 -0.31405402835204654 1104 8 -0.26135666953732084 1104 9 -0.3081760684573567 1104 10-11 -0.35684124611409374 1104 12-13 -0.37508622241482925 1104 14-15 -0.5277601561928833 1104 16-17 -0.5808007894442895 1104 18-19 -0.6082549448780412 1104 20-21 -0.5421203556754293 1104 22-23 -0.5057196224973168 1104 24-25 -0.5341356447475647 1104 26-27 -0.6633776823270026 1104 28-29 -0.6170639843006 1104 30-31 -0.6310724500923541 1104 32-33 -0.7455144424207063 1104 34-35 -0.689311461178157 1104 36-37 -0.7554196321341564 1104 38-39 -0.7555648909829102 1104 40-41 -0.852168603487705 1104 42-43 -0.7728583807235765 1104 44-45 -0.8161213423735347 1104 46-47 -0.8556801909645344 1104 48-49 -0.8098103066891298 1104 50-51 -0.7662207215723313 1104 52-53 -0.7199960052004997 1104 54-55 -0.6846550877644901 1104 56-57 -0.579477472126726 1104 58-59 -0.6909794663348379 1104 60-61 -0.49777472603366135 1104 62-63 -0.5663054939510204 1104 64-65 -0.6627575757702147 1104 66-67 -0.6528526773164245 1104 68-69 -0.616478145484308 1104 70-71 -0.6420944985577037 1104 72-73 -0.5895366450683213 1104 74-75 -0.5761598514193835 1104 76-77 -0.6533248685063953 1104 78-79 -0.6759207771768239 1104 80-81 -0.6858946248177311 1104 82-83 -0.709861239454824 1104 84-85 -0.7930749829167496 1104 86-87 -0.7924165914096122 1104 88-89 -0.7982249748904842 1104 90-91 -0.7788862453743128 1104 92-93 -0.7595405904972807 1104 94-95 -0.8974652973570123 1104 96-97 -0.9192754993023975 1104 98-99 -0.8412862749830943 1104 100 -1.1101041780617535 1105 1 -0.3501452921516268 1105 2 -0.3897666157253106 1105 3 -0.38474796674983835 1105 4 -0.32431015992779066 1105 5 -0.2207438724420001 1105 6 -0.28168859554476455 1105 7 -0.2354128361050698 1105 8 -0.25751729446798777 1105 9 -0.34670566848487994 1105 10-11 -0.4232422798741382 1105 12-13 -0.41755493405889865 1105 14-15 -0.5918324713443539 1105 16-17 -0.6440626802653213 1105 18-19 -0.5661346066903192 1105 20-21 -0.6090722762824683 1105 22-23 -0.6647998602158509 1105 24-25 -0.5512800235216133 1105 26-27 -0.5666686769398908 1105 28-29 -0.6958930816855897 1105 30-31 -0.7061728095295123 1105 32-33 -0.612598771636442 1105 34-35 -0.7768122249599045 1105 36-37 -0.7208578411534887 1105 38-39 -0.7035779758246719 1105 40-41 -0.8008799924670669 1105 42-43 -0.7850503393921571 1105 44-45 -0.7624878472667831 1105 46-47 -0.632242295169867 1105 48-49 -0.7252116168936666 1105 50-51 -0.7883250841156411 1105 52-53 -0.5842917895405719 1105 54-55 -0.4814984149223065 1105 56-57 -0.5736179314398484 1105 58-59 -0.4453198970543468 1105 60-61 -0.5205001246980672 1105 62-63 -0.5498805530810742 1105 64-65 -0.5648413503172733 1105 66-67 -0.5457819715939678 1105 68-69 -0.5275875214019621 1105 70-71 -0.5578376567047201 1105 72-73 -0.5668663425039782 1105 74-75 -0.5920532595232402 1105 76-77 -0.5978789119224217 1105 78-79 -0.5309406918586994 1105 80-81 -0.6421401644806899 1105 82-83 -0.7749517572306175 1105 84-85 -0.7098663017290328 1105 86-87 -0.7363736244601533 1105 88-89 -0.7502169176605378 1105 90-91 -0.6586069355838475 1105 92-93 -0.6881393075812561 1105 94-95 -0.6948234727284159 1105 96-97 -0.8526260510939174 1105 98-99 -0.8313538496453532 1105 100 -0.9036575339259088 1106 1 -0.4916010470065473 1106 2 -0.5329194224729186 1106 3 -0.5237404442367257 1106 4 -0.3745786109726126 1106 5 -0.3677697290582529 1106 6 -0.45639021036973304 1106 7 -0.4879049443798351 1106 8 -0.47003343830479594 1106 9 -0.5378080976358675 1106 10-11 -0.6130326821241212 1106 12-13 -0.5064261305121036 1106 14-15 -0.6674046030368785 1106 16-17 -0.6880225317567294 1106 18-19 -0.708523394409525 1106 20-21 -0.6905288548775772 1106 22-23 -0.6785738259070726 1106 24-25 -0.6991421235295547 1106 26-27 -0.6659098062876936 1106 28-29 -0.8212583957512507 1106 30-31 -0.7893451948163417 1106 32-33 -0.7749698048125779 1106 34-35 -0.7832346036273847 1106 36-37 -0.7677179200560289 1106 38-39 -0.8174870496170854 1106 40-41 -0.7298629969417405 1106 42-43 -0.8167674258231017 1106 44-45 -0.8279370210259458 1106 46-47 -0.7786822665743145 1106 48-49 -0.7650014307578061 1106 50-51 -0.8670278698284548 1106 52-53 -0.6558365671748447 1106 54-55 -0.7404140050880343 1106 56-57 -0.7035899389829581 1106 58-59 -0.6476994029867456 1106 60-61 -0.6935908126093864 1106 62-63 -0.6044709188511419 1106 64-65 -0.7768441274984852 1106 66-67 -0.7802234316146226 1106 68-69 -0.6783235185541727 1106 70-71 -0.6204862609063824 1106 72-73 -0.5288790486964459 1106 74-75 -0.6142526899657383 1106 76-77 -0.7172689470196367 1106 78-79 -0.7217446622864223 1106 80-81 -0.8112383601954782 1106 82-83 -0.6930035087082018 1106 84-85 -0.6871079047274478 1106 86-87 -0.7561525090570953 1106 88-89 -0.8392119693355262 1106 90-91 -0.7178342231544903 1106 92-93 -0.8250539739811877 1106 94-95 -0.7713887997385633 1106 96-97 -0.8372379223668638 1106 98-99 -0.8439200798277824 1106 100 -0.9199249317600611 1107 1 -0.5854548539702975 1107 2 -0.5883255714716888 1107 3 -0.5579512747091862 1107 4 -0.4094774328151374 1107 5 -0.4101413541148915 1107 6 -0.47281644926928124 1107 7 -0.46998605441238794 1107 8 -0.44259711802217794 1107 9 -0.6129878112498801 1107 10-11 -0.6200424343567761 1107 12-13 -0.6437505522166092 1107 14-15 -0.7940086250371934 1107 16-17 -0.7680276281441962 1107 18-19 -0.6955399180953776 1107 20-21 -0.7338884336990574 1107 22-23 -0.7971645428603296 1107 24-25 -0.8571653114643922 1107 26-27 -0.856124323579813 1107 28-29 -0.8332534995341305 1107 30-31 -0.7405382813175763 1107 32-33 -0.7836476712478699 1107 34-35 -0.8588989556323909 1107 36-37 -0.9504679257093755 1107 38-39 -0.9115139955618758 1107 40-41 -0.8812484019265625 1107 42-43 -0.9001800037241168 1107 44-45 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0.8008597491723108 2311 16-17 0.7811316192308198 2311 18-19 0.6835367687427691 2311 20-21 0.7188857806979101 2311 22-23 0.7684883654008416 2311 24-25 0.7823108904003675 2311 26-27 0.8359345945902561 2311 28-29 0.8304369016830933 2311 30-31 0.8059557534815625 2311 32-33 0.836455189877249 2311 34-35 0.8295297846805383 2311 36-37 0.8486501328134395 2311 38-39 0.8908251360231532 2311 40-41 0.9131989146446315 2311 42-43 0.9739661352170614 2311 44-45 0.9771961920015428 2311 46-47 0.9562504031934722 2311 48-49 0.9889784714931196 2311 50-51 1.0629463169648474 2311 52-53 0.9239901031237849 2311 54-55 0.9063009844642664 2311 56-57 0.8653917926189862 2311 58-59 0.8360175679846407 2311 60-61 0.838579074982313 2311 62-63 0.7737128843644072 2311 64-65 0.7684383267407071 2311 66-67 0.7945809115332665 2311 68-69 0.8181749483834011 2311 70-71 0.7602456940019451 2311 72-73 0.7561061560565889 2311 74-75 0.7858094190301017 2311 76-77 0.7670706659810733 2311 78-79 0.6768926368729815 2311 80-81 0.7532092316008026 2311 82-83 0.7595407976852471 2311 84-85 0.8469892189886501 2311 86-87 0.8666834070501572 2311 88-89 0.9018030833110693 2311 90-91 0.9057858288346559 2311 92-93 0.9168274481482754 2311 94-95 0.9815291633723753 2311 96-97 0.9804165444409669 2311 98-99 0.99126998084947 2311 100 1.1768065812440263 2312 1 0.45422371374317194 2312 2 0.4754410595237104 2312 3 0.5133528801312757 2312 4 0.4085187464199862 2312 5 0.4393968992788686 2312 6 0.445555882130634 2312 7 0.5131665125520186 2312 8 0.4762512254419491 2312 9 0.6087614079267283 2312 10-11 0.6140650613140508 2312 12-13 0.6147625202981573 2312 14-15 0.7639803345856535 2312 16-17 0.7781380731694583 2312 18-19 0.7951829868240452 2312 20-21 0.7284760516481654 2312 22-23 0.7514723276799415 2312 24-25 0.8232381207001254 2312 26-27 0.8798721216231868 2312 28-29 0.817720003228736 2312 30-31 0.8192830182051836 2312 32-33 0.8153096072706134 2312 34-35 0.805512064881853 2312 36-37 0.87841058946087 2312 38-39 0.8716561750081553 2312 40-41 0.8514327342467922 2312 42-43 0.8572605935912492 2312 44-45 0.8610758659520741 2312 46-47 0.9414568163972845 2312 48-49 0.8667538338827896 2312 50-51 0.8672875593094957 2312 52-53 0.6848833682802677 2312 54-55 0.7010035023363841 2312 56-57 0.5629772513375073 2312 58-59 0.5730295167078623 2312 60-61 0.6665151161636587 2312 62-63 0.6771600583666739 2312 64-65 0.6694118320585076 2312 66-67 0.609454919891725 2312 68-69 0.6531649690031642 2312 70-71 0.6649414383960845 2312 72-73 0.6496331237707054 2312 74-75 0.6834216604859549 2312 76-77 0.7101972757118133 2312 78-79 0.6694424811241753 2312 80-81 0.7926805781413968 2312 82-83 0.7818103609500255 2312 84-85 0.8073145016599383 2312 86-87 0.8670290633343569 2312 88-89 0.8934010521224884 2312 90-91 0.8652729572587354 2312 92-93 0.9899964491391948 2312 94-95 0.9673391508888578 2312 96-97 0.9591593494631887 2312 98-99 1.0158852799835323 2312 100 1.1257399964983712 2313 1 0.49313106929590944 2313 2 0.49915324599171385 2313 3 0.5364687628723104 2313 4 0.4367763424293898 2313 5 0.41073786710006743 2313 6 0.3882846219900955 2313 7 0.4400497747339429 2313 8 0.3921728499803194 2313 9 0.5394457125825767 2313 10-11 0.5738539382918688 2313 12-13 0.6045103715795577 2313 14-15 0.7615003861422949 2313 16-17 0.7425200639868308 2313 18-19 0.7225727696434774 2313 20-21 0.7225197649867425 2313 22-23 0.6796314548172901 2313 24-25 0.6865182772422287 2313 26-27 0.7622646001855102 2313 28-29 0.7515683179618975 2313 30-31 0.8459929247677636 2313 32-33 0.9447954989494534 2313 34-35 0.939492736540366 2313 36-37 0.9399790617802282 2313 38-39 0.9718403766047174 2313 40-41 0.9916367916323665 2313 42-43 0.8416351857419997 2313 44-45 0.9410633728264486 2313 46-47 1.0382571631085327 2313 48-49 1.030391052596194 2313 50-51 0.9691560376593884 2313 52-53 0.9105213232599993 2313 54-55 0.832775825022658 2313 56-57 0.7917841668405927 2313 58-59 0.8085910908056917 2313 60-61 0.8520679594748444 2313 62-63 0.8637387691085081 2313 64-65 0.8485041182735031 2313 66-67 0.8138802136106946 2313 68-69 0.8570571896922488 2313 70-71 0.8374527816681194 2313 72-73 0.8446869981247858 2313 74-75 0.8994447241639918 2313 76-77 0.8133400438752219 2313 78-79 0.8484895382640296 2313 80-81 0.9252850258021219 2313 82-83 0.8047518529784554 2313 84-85 0.8644064206418385 2313 86-87 0.9210559811596504 2313 88-89 0.8676866352535306 2313 90-91 0.8654779507286428 2313 92-93 0.9616641463987214 2313 94-95 0.9965231553676581 2313 96-97 1.001267168377673 2313 98-99 0.9988336936355999 2313 100 1.069561434809966 2314 1 0.3277494556640015 2314 2 0.17036157102468508 2314 3 0.26047403282421655 2314 4 0.19316874907060821 2314 5 0.19384101320068226 2314 6 0.25368243695617565 2314 7 0.29897008167822037 2314 8 0.3105988903653767 2314 9 0.4163691066555799 2314 10-11 0.4195335273782632 2314 12-13 0.43859779714156844 2314 14-15 0.506784732869896 2314 16-17 0.5775363835229683 2314 18-19 0.5893263369424773 2314 20-21 0.5436879780013655 2314 22-23 0.5666144412379452 2314 24-25 0.5697566213787439 2314 26-27 0.6215651906870292 2314 28-29 0.5964446783031292 2314 30-31 0.6079859260818665 2314 32-33 0.6800006067911966 2314 34-35 0.7091139198170779 2314 36-37 0.6648156384867221 2314 38-39 0.7280158039734062 2314 40-41 0.6862313754327545 2314 42-43 0.6613607699965627 2314 44-45 0.7533777745619261 2314 46-47 0.6649190605477884 2314 48-49 0.6798406752011275 2314 50-51 0.7692972506826479 2314 52-53 0.7602385179989923 2314 54-55 0.7373938588019584 2314 56-57 0.7914085831225819 2314 58-59 0.733521668330809 2314 60-61 0.599903612915071 2314 62-63 0.7216028426580507 2314 64-65 0.76957413087068 2314 66-67 0.7230747813872611 2314 68-69 0.7558625107332162 2314 70-71 0.7651387155778053 2314 72-73 0.7825456432591693 2314 74-75 0.7568051282484944 2314 76-77 0.7844294367536193 2314 78-79 0.7758606232878655 2314 80-81 0.8413481161210932 2314 82-83 0.7975835492750889 2314 84-85 0.8048510867519489 2314 86-87 0.8453395542089872 2314 88-89 0.722531758002436 2314 90-91 0.7232987575179699 2314 92-93 0.9140677652023008 2314 94-95 0.9424805110033248 2314 96-97 0.957436649340341 2314 98-99 0.890641660060755 2314 100 0.9338409704351882 2315 1 0.29151581496504875 2315 2 0.31879957767394984 2315 3 0.38031377506081654 2315 4 0.27013353550309205 2315 5 0.28454385846278996 2315 6 0.25705678815027966 2315 7 0.21126418173555805 2315 8 0.2696367109102553 2315 9 0.07062744044011993 2315 10-11 0.33120561204078314 2315 12-13 0.3029117057002324 2315 14-15 0.49681329226869053 2315 16-17 0.2894875015313829 2315 18-19 0.4971928052553949 2315 20-21 0.38510346324228095 2315 22-23 0.3933464580487751 2315 24-25 0.5240525990173808 2315 26-27 0.5847221629349164 2315 28-29 0.5544897230279915 2315 30-31 0.5581299686835521 2315 32-33 0.5866099786850469 2315 34-35 0.5088630729827415 2315 36-37 0.6323748201564072 2315 38-39 0.713075336202138 2315 40-41 0.7341180469721351 2315 42-43 0.7805041976655929 2315 44-45 0.7223029704410209 2315 46-47 0.7831255129704644 2315 48-49 0.6135084091415521 2315 50-51 0.6306398018838166 2315 52-53 0.39894811005532205 2315 54-55 0.5564282255682755 2315 56-57 0.5407545860881342 2315 58-59 0.5752198402584341 2315 60-61 0.6173854118704369 2315 62-63 0.676768227952202 2315 64-65 0.677377894763147 2315 66-67 0.5353558939969005 2315 68-69 0.6197336720188176 2315 70-71 0.5662463894301197 2315 72-73 0.5943404123270852 2315 74-75 0.35606637158704757 2315 76-77 0.6270244064544812 2315 78-79 0.6946909568666406 2315 80-81 0.6931813206665502 2315 82-83 0.6401944137249629 2315 84-85 0.6834390033451037 2315 86-87 0.6148440278884628 2315 88-89 0.556745391432873 2315 90-91 0.6814963759319959 2315 92-93 0.7711345229096409 2315 94-95 0.7661532756086231 2315 96-97 0.7979169813674005 2315 98-99 0.8796042509374438 2315 100 0.8797163354081761 2316 1 0.16167048341708323 2316 2 0.20591893700607145 2316 3 0.18028244825800854 2316 4 0.09604336108000666 2316 5 0.06397851816775812 2316 6 -0.033036147329148946 2316 7 -0.02064732836802108 2316 8 0.024993183381639028 2316 9 0.10723637513078899 2316 10-11 0.09796206763741111 2316 12-13 0.08881834171565117 2316 14-15 -0.008156901447655684 2316 16-17 0.17622114828724023 2316 18-19 0.24883721223080357 2316 20-21 0.3006385316703799 2316 22-23 0.177938584972253 2316 24-25 0.17252713435051703 2316 26-27 0.14212072108036722 2316 28-29 0.09284651740492933 2316 30-31 -0.4580069161724296 2316 32-33 -0.24751167738416058 2316 34-35 -0.40385964067679225 2316 36-37 -0.18272502007057767 2316 38-39 -0.014459809542280766 2316 40-41 0.17515861296971735 2316 42-43 0.2464549703442458 2316 44-45 0.10892468497146979 2316 46-47 -0.05621600848407127 2316 48-49 0.0370552494532248 2316 50-51 0.17069216999629333 2316 52-53 -0.17622435326366315 2316 54-55 -0.12675435849900651 2316 56-57 -0.13432411012698964 2316 58-59 -0.48379640912482813 2316 60-61 -0.0231216188584753 2316 62-63 0.05013513138516856 2316 64-65 -0.3012859612692296 2316 66-67 -0.5872412301520455 2316 68-69 -0.5700036689613128 2316 70-71 -0.3469800258271931 2316 72-73 -0.591298887241841 2316 74-75 -0.4206583985391035 2316 76-77 -0.05217622918257092 2316 78-79 -0.05847464078429354 2316 80-81 -0.1144063543537932 2316 82-83 0.14690770069237757 2316 84-85 0.2211720662258152 2316 86-87 0.31328839511022366 2316 88-89 0.34196207937030465 2316 90-91 -0.3371141307869401 2316 92-93 -0.4338771229925733 2316 94-95 0.07157888509084742 2316 96-97 0.411990271022983 2316 98-99 0.41456484173524544 2316 100 0.43356923194714625 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 214.0 1 567.0 2 311.0 3 332.0 4 304.0 5 280.0 6 341.0 7 888.0 8 2269.0 9 3687.0 10 5583.0 11 9280.0 12 13654.0 13 17811.0 14 20797.0 15 24296.0 16 28281.0 17 34281.0 18 41488.0 19 49127.0 20 58564.0 21 69868.0 22 78659.0 23 81706.0 24 82839.0 25 85853.0 26 93527.0 27 106648.0 28 123743.0 29 147165.0 30 177975.0 31 217842.0 32 275376.0 33 356068.0 34 488215.0 35 677094.0 36 993684.0 37 1429662.0 38 1322019.0 39 309069.0 40 263.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.71424872953138 18.661399227305203 13.19539872728979 23.42895331587363 2 15.899214898667152 19.97000539660317 40.55504838415191 23.575731320577766 3 19.13107018565077 25.665961620579303 29.493389923692405 25.709578270077525 4 12.951111136240245 16.3811974669295 37.710164254337165 32.95752714249309 5 13.570354758052932 37.18824097522217 34.28875061876248 14.952653647962416 6 30.918050296799 36.296574389347604 18.324042402088377 14.461332911765016 7 27.706658447176107 32.331414599371875 22.067972116751776 17.89395483670024 8 26.151281640435126 34.977520631334315 20.228119475252498 18.643078252978064 9 26.843000341045155 16.241248852692056 20.54621350342237 36.36953730284042 10-11 25.178346726435024 26.009642925499627 27.92915290110114 20.882857446964213 12-13 25.598138500807266 23.721410916535085 28.152926712389544 22.527523870268105 14-15 23.13928155923589 25.166073605152032 26.001022716394523 25.69362211921755 16-17 22.470435948948932 27.99260677396862 26.73800830869452 22.79894896838793 18-19 22.218378810994132 27.13879495234286 28.834446198365487 21.80838003829752 20-21 23.128991036808248 26.39218049166766 28.659772463139603 21.81905600838449 22-23 23.061032145662402 26.1347506222308 28.487478560423952 22.316738671682845 24-25 22.597721994734464 26.21453601308336 28.748922094132457 22.43881989804972 26-27 22.477546022052444 26.421528614537948 28.51638701687299 22.58453834653662 28-29 22.36997572262493 26.705644503170276 28.6108286142289 22.31355115997589 30-31 22.92533990288018 26.43471438758634 28.512769658798636 22.127176050734843 32-33 22.72544386411023 26.59134904634457 28.346930371923197 22.33627671762201 34-35 22.811220628012705 26.6398078922999 28.40788036924688 22.141091110440517 36-37 22.812084539830618 26.537913329323885 28.23018840511702 22.419813725728474 38-39 22.74541115864987 26.662921827939133 28.316293037872153 22.275373975538837 40-41 22.770238513676393 26.305798746762875 28.557572011306874 22.366390728253858 42-43 22.63764601012771 26.48103934707428 28.203455687212774 22.677858955585233 44-45 22.473274423882064 26.748787496696234 28.262893947836687 22.515044131585014 46-47 22.500192925577327 26.53561688980266 27.932903110418213 23.031287074201803 48-49 22.709942837626212 26.577520712000435 27.89384180605304 22.818694644320313 50-51 22.665065536556465 26.800407806061433 27.761361944578532 22.77316471280357 52-53 22.529267876152346 26.993517995750494 27.588256161998387 22.888957966098772 54-55 22.423711967923957 26.80400306846883 27.77294892768892 22.999336035918294 56-57 22.62350305827293 26.8029844935324 27.531755543673103 23.041756904521566 58-59 22.420067111676268 26.813548788276364 27.875148176615415 22.891235923431953 60-61 22.680394948332776 26.921368265808276 27.65347999672501 22.744756789133938 62-63 22.663505934621195 26.850661134375553 27.615485566955467 22.87034736404778 64-65 22.42687102862942 26.63767525743208 27.740959442192793 23.19449427174571 66-67 22.545487844826397 26.567688976766313 27.60680322082053 23.280019957586763 68-69 22.24846107130056 26.669704896445317 27.779089720231692 23.302744312022426 70-71 22.376048907068057 26.495781746525548 27.744888185711968 23.383281160694423 72-73 22.41496235606806 26.768513976284037 27.528921064743656 23.287602602904244 74-75 22.485012992118005 26.827338316961352 27.461400538941778 23.22624815197886 76-77 22.415154045040335 26.686706348765476 27.427990093682585 23.470149512511604 78-79 22.35368873039133 26.65041571377672 27.53279275873449 23.463102797097456 80-81 22.35947935606572 26.852680064018212 27.426782047776086 23.36105853213998 82-83 22.68394575866549 26.61028961821633 27.595425717123707 23.11033890599447 84-85 22.751369531444595 26.699366762786774 27.27143438844587 23.277829317322755 86-87 22.400200015433455 26.855748497695824 27.49932091447401 23.244730572396712 88-89 22.537576083833937 26.415516556588404 27.588136571837158 23.458770787740498 90-91 22.780791166063413 26.38896652267862 27.56171863835466 23.268523672903303 92-93 22.762325786260096 26.48239442785426 27.45061671024115 23.30466307564449 94-95 22.502030623007265 26.360032489968116 27.57480303293583 23.56313385408879 96-97 22.591904067928088 26.277616781539386 27.548338775417015 23.58214037511551 98-99 22.518403209051684 26.361299987613663 27.398768626907398 23.721528176427256 100 22.784643968122236 26.254600009110398 27.17516786175485 23.785588161012516 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2161.0 1 1602.0 2 2441.5 3 3425.0 4 2325.0 5 1767.0 6 2462.0 7 4276.0 8 6752.0 9 8090.0 10 7240.0 11 6134.0 12 6121.5 13 6545.5 14 7152.5 15 7765.0 16 8261.0 17 8792.5 18 9472.5 19 10242.0 20 11092.0 21 12210.5 22 13738.0 23 15907.5 24 18974.5 25 22889.0 26 28197.0 27 34700.0 28 42430.0 29 53099.0 30 67266.0 31 81741.0 32 98546.5 33 117431.5 34 135865.0 35 154115.5 36 175311.0 37 198513.0 38 211028.0 39 215042.0 40 218208.5 41 221438.0 42 227689.5 43 238085.0 44 250274.0 45 266198.5 46 281623.0 47 291676.0 48 300474.0 49 307958.0 50 307009.5 51 299880.0 52 291079.5 53 279565.0 54 266173.0 55 246071.5 56 222632.0 57 197089.0 58 172717.0 59 150625.0 60 125550.0 61 102256.0 62 82638.0 63 65559.5 64 49680.0 65 36426.5 66 26996.0 67 20532.5 68 15819.0 69 11812.0 70 9053.0 71 6916.0 72 5301.0 73 4048.0 74 3091.5 75 2273.5 76 1586.0 77 1066.0 78 685.0 79 465.5 80 335.0 81 236.5 82 170.5 83 122.0 84 95.5 85 84.0 86 65.0 87 47.0 88 37.0 89 36.0 90 31.5 91 27.0 92 21.0 93 17.5 94 15.0 95 10.0 96 5.0 97 2.5 98 1.5 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025021434445591503 2 0.11164755176233541 3 0.01714755647320257 4 0.01794167408067427 5 0.0201490518370363 6 0.0456550326193902 7 0.04548005755333711 8 0.03795612971305435 9 0.03312412596589601 10-11 0.036805332163243665 12-13 0.036515950323232786 14-15 0.031643567714677585 16-17 0.038110915348409005 18-19 0.03739755546373103 20-21 0.04759994777667259 22-23 0.04747208138224918 24-25 0.06638957794668106 26-27 0.05829361623660936 28-29 0.05095812308284531 30-31 0.049107425268822266 32-33 0.043851443476996835 34-35 0.04548005755333711 36-37 0.04038559120709914 38-39 0.04588384616730577 40-41 0.054646059090425775 42-43 0.05599875094722079 44-45 0.05912138289524512 46-47 0.06039331702924641 48-49 0.06011066499946834 50-51 0.05439705611181177 52-53 0.06414855113915498 54-55 0.0508302566884219 56-57 0.0553930680262678 58-59 0.05313185178804328 60-61 0.04907377621765822 62-63 0.05565553062534742 64-65 0.05359620869410725 66-67 0.05225697645777785 68-69 0.05725049565052365 70-71 0.05763409483379387 72-73 0.06006355632783867 74-75 0.05089755479075001 76-77 0.05200124366893102 78-79 0.06231131294559756 80-81 0.06131530103114152 82-83 0.05187337727450761 84-85 0.05883873086546705 86-87 0.05657078481700974 88-89 0.06408125303682687 90-91 0.06959969742773194 92-93 0.07483548978885893 94-95 0.07806579870060823 96-97 0.07461340605117617 98-99 0.0824872840235651 100 0.12835094076017245 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 7429630.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.56334258095966 #Duplication Level Percentage of deduplicated Percentage of total 1 75.29107040811684 44.84587820005817 2 14.60409302325515 17.39737191656699 3 4.766191661292389 8.516709201842156 4 1.9818226451568863 4.7217592459273305 5 0.9986132037168964 2.9740370179429583 6 0.5796671450654602 2.071614764667651 7 0.3775850253934072 1.5743158354663511 8 0.26418035027785514 1.2588371765406245 9 0.17539497908493049 0.9402400103594373 >10 0.8911653404698131 9.230507789050248 >50 0.04544646421074833 1.8590295054370525 >100 0.02286850371241059 2.4316636323258667 >500 0.0013368223781110129 0.5287303183216117 >1k 4.45600949378923E-4 0.5170457636493611 >5k 2.9706729948830902E-5 0.09052414791928012 >10k+ 8.912018984649272E-5 1.0417354739248823 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 26085 0.35109419984575274 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20762 0.2794486401072463 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12027 0.16187885534003713 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.345962046562211E-5 2 0.0 0.0 0.0 0.0 1.345962046562211E-5 3 0.0 0.0 0.0 0.0 1.345962046562211E-5 4 0.0 0.0 0.0 1.345962046562211E-5 2.691924093124422E-5 5 0.0 2.691924093124422E-5 0.0 1.345962046562211E-5 2.691924093124422E-5 6 0.0 2.691924093124422E-5 0.0 1.345962046562211E-5 6.729810232811055E-5 7 0.0 2.691924093124422E-5 0.0 2.691924093124422E-5 6.729810232811055E-5 8 0.0 2.691924093124422E-5 0.0 2.691924093124422E-5 8.075772279373266E-5 9 0.0 2.691924093124422E-5 0.0 4.037886139686633E-5 9.421734325935477E-5 10-11 0.0 2.691924093124422E-5 0.0 8.748753302654372E-5 9.421734325935477E-5 12-13 0.0 2.691924093124422E-5 0.0 1.21136584190599E-4 9.421734325935477E-5 14-15 0.0 3.3649051164055275E-5 0.0 1.21136584190599E-4 1.5478563535465428E-4 16-17 0.0 4.037886139686633E-5 0.0 1.2786639442341006E-4 2.2881354791557589E-4 18-19 0.0 4.037886139686633E-5 0.0 1.4132601488903217E-4 2.557327888468201E-4 20-21 0.0 4.037886139686633E-5 0.0 2.3554335814838693E-4 2.557327888468201E-4 22-23 0.0 4.037886139686633E-5 0.0 5.249251981592623E-4 2.8265202977806434E-4 24-25 0.0 4.037886139686633E-5 0.0 0.0013930707181918885 3.2303089117493065E-4 26-27 0.0 4.037886139686633E-5 0.0 0.002792871246616588 3.2303089117493065E-4 28-29 0.0 5.383848186248844E-5 0.0 0.00733549315376405 3.2303089117493065E-4 30-31 0.0 5.383848186248844E-5 0.0 0.021918991928265606 3.297607014077417E-4 32-33 2.691924093124422E-5 5.383848186248844E-5 0.0 0.055547853661622446 3.4322032187336383E-4 34-35 2.691924093124422E-5 5.383848186248844E-5 0.0 0.10640502959097559 4.037886139686633E-4 36-37 2.691924093124422E-5 6.729810232811055E-5 0.0 0.18586389900977573 4.1051842420147436E-4 38-39 2.691924093124422E-5 8.748753302654372E-5 0.0 0.30191274666436957 4.3070785489990755E-4 40-41 2.691924093124422E-5 9.421734325935477E-5 0.0 0.42822455492400024 4.3070785489990755E-4 42-43 2.691924093124422E-5 9.421734325935477E-5 0.0 0.5522078488430783 4.441674753655297E-4 44-45 2.691924093124422E-5 9.421734325935477E-5 0.0 0.6994089880653545 4.5762709583115177E-4 46-47 2.691924093124422E-5 9.421734325935477E-5 0.0 0.8760395874357135 4.7108671629677386E-4 48-49 2.691924093124422E-5 9.421734325935477E-5 0.0 1.0630408243748342 4.980059572280181E-4 50-51 2.691924093124422E-5 9.421734325935477E-5 0.0 1.2557489403913786 4.980059572280181E-4 52-53 2.691924093124422E-5 9.421734325935477E-5 0.0 1.4464583027687785 5.518444390905065E-4 54-55 2.691924093124422E-5 1.2786639442341006E-4 0.0 1.660755650012181 6.056829209529949E-4 56-57 2.691924093124422E-5 1.345962046562211E-4 0.0 1.9080761760679872 6.056829209529949E-4 58-59 2.691924093124422E-5 1.345962046562211E-4 0.0 2.1874238690217416 6.056829209529949E-4 60-61 2.691924093124422E-5 1.345962046562211E-4 0.0 2.469773057339329 6.056829209529949E-4 62-63 2.691924093124422E-5 1.345962046562211E-4 0.0 2.76333410950478 6.191425414186171E-4 64-65 2.691924093124422E-5 1.4132601488903217E-4 0.0 3.0572518416125702 6.393319721170502E-4 66-67 2.691924093124422E-5 1.6151544558746532E-4 0.0 3.390074606676241 6.460617823498613E-4 68-69 2.691924093124422E-5 1.7497506605308744E-4 0.0 3.75023386090559 6.460617823498613E-4 70-71 2.691924093124422E-5 1.7497506605308744E-4 0.0 4.1276550783821 6.527915925826724E-4 72-73 2.691924093124422E-5 1.7497506605308744E-4 0.0 4.49174454178741 7.33549315376405E-4 74-75 2.691924093124422E-5 1.7497506605308744E-4 0.0 4.851890336396295 7.402791256092161E-4 76-77 2.691924093124422E-5 1.7497506605308744E-4 0.0 5.248377106262358 7.739281767732713E-4 78-79 2.691924093124422E-5 1.7497506605308744E-4 0.0 5.684906247013647 7.941176074717046E-4 80-81 2.691924093124422E-5 1.7497506605308744E-4 0.0 6.1363351876203795 8.075772279373266E-4 82-83 2.691924093124422E-5 1.8843468651870955E-4 0.0 6.587743938796414 8.748753302654372E-4 84-85 2.691924093124422E-5 2.0189430698433166E-4 0.0 7.041716747671149 8.883349507310593E-4 86-87 2.691924093124422E-5 2.557327888468201E-4 0.0 7.515569415973609 9.017945711966813E-4 88 2.691924093124422E-5 2.557327888468201E-4 0.0 7.891429317476105 9.556330530591698E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 12025 0.0 52.890797 1 GTATCAA 21770 0.0 41.640102 1 TACCTGG 5600 0.0 35.6163 2 ATCAACG 27175 0.0 32.697163 3 CAACGCA 27275 0.0 32.575962 5 TATCAAC 27725 0.0 32.423515 2 TCAACGC 28080 0.0 31.776793 4 AACGCAG 29195 0.0 30.884455 6 GTGGTAT 5350 0.0 27.785728 1 GTACATG 29340 0.0 27.305056 1 ACGCAGA 32505 0.0 27.293755 7 GTACCTG 7575 0.0 26.765978 1 CGCAGAG 33370 0.0 26.487331 8 TACATGG 30190 0.0 26.021019 2 ACCTGGG 7625 0.0 25.635485 3 ACATGGG 30395 0.0 24.811954 3 GCAGAGT 36325 0.0 23.621931 9 CATGGGG 21800 0.0 23.148882 4 TGGTATC 6330 0.0 22.516966 2 GTACTAG 2350 0.0 22.420158 1 >>END_MODULE